MD16G1242100.v1.1

Endonuclease I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003467
Physical Location & Seq
Reverse (-)
26194420 .. 26197150
2731 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1242100.v1.1.491

Sequence Viewer

Length: 987 bp
ATGCAGCATCCCGTTGTGGTGGCCACCAAATTCCACTGCACAAAATCTGTCGTCGACAGCACCGCCGTCCGCAAGCAGAGCGCCACCTTCTCCCGCAGCCGCGGCGAACACTTCAAATCCTCATTCCAATTAGCTTGCAACTATCGCAACCCAGATAATAATTTGGTCTCTCCAACCACTTCCCTCATTTCTACAAGATGGAATTACAGAAAATGCATCAGTGTTTCTTGGAGATGGGTTTCTCAGATCATCTCTTTTTATTTGTTGTTTCTCAATGTTGTTGCTGATGCTGAGGCATTTGATGATCCATCTCCTTCTTTTATGTATGATTGTGAAGATGTTAGCAATTATTATGACAGTGCGGAGAATTTAGAGGGCAAATCGCTCAAGAAAAAATTGAATTCCATTATTTCCGGGCACCAATCTTTATCCTACAGAGAGGTGTGGAAAGCTCTCAAGATTCTTGATGCTTCGGATGTTGACAACCCAGAAGCTTCGTCAGGAATGTATATGCTGAATACTGCTTCCTGCATTGTTTTTTGTACTATCAAATATACCCTGCAACTCTTACCAATTCGCCTACCGACTCTTTTATGCAGAGTTGAAGTTTACTCCTTGAGGGTTGTACCCAAGTCTTTAGCTGGGAAGCCCGAAGGATGGAATAGGGAGCATCTATGGCCTCGTTCTTATGGGTTAAAAGATGGTGCGTCTTTGACAGATATACACAACATTCGCCCAGCAGATGTGAATGTCAATGCATCCAGGGGGAACAAATACTATGGAGAATGCACGGGTAACTCAGCTAAATGTTTGAAGCCAGCAAACAAAGAAGCCGCTTTGGACACGGAAACTGACAAGGACAGATGGGCACCACCTAAACAGTTCTTATTGATATGGGAATGGCCAAATGGTGTAGTTCCTTTTGAAGTGTGGATCCGTTGTCTCCTTGATCGAAAGGAGTGTTTGACTGAGGTTCTAGGATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endonuclease_1

Protein Analysis

329

Amino Acids

37.27

Weight (kDa)

8.54

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_1 PF04231 126 - 268 1.6e-13 Endonuclease I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 417, 868
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 102
AciI CCGC 7 cut(s) 63, 70, 94, 100, 102, 362, 834
AclWI GGATC 3 cut(s) 299, 928, 941
AcoI YGGCCR 2 cut(s) 21, 902
AcsI RAATTY 3 cut(s) 29, 367, 400
AfaI GTAC 2 cut(s) 544, 627
AfiI CCNNNNNNNGG 1 cut(s) 657
AgsI TTSAA 5 cut(s) 115, 400, 605, 814, 926
AjnI CCWGG 1 cut(s) 761
AluBI AGCT 5 cut(s) 134, 452, 494, 641, 803
AluI AGCT 5 cut(s) 134, 452, 494, 641, 803
Alw26I GTCTC 2 cut(s) 172, 947
AlwI GGATC 3 cut(s) 299, 928, 941
AoxI GGCC 3 cut(s) 21, 677, 902
ApeKI GCWGC 2 cut(s) 4, 96
ApoI RAATTY 3 cut(s) 29, 367, 400
AspLEI GCGC 1 cut(s) 83
AsuC2I CCSGG 1 cut(s) 415
BaeGI GKGCMC 2 cut(s) 420, 871
BalI TGGCCA 2 cut(s) 23, 904
BamHI GGATCC 1 cut(s) 933
BanI GGYRCC 2 cut(s) 417, 868
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 2 cut(s) 16, 108
BccI CCATC 6 cut(s) 192, 228, 316, 651, 695, 858
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 763
BcnI CCSGG 1 cut(s) 415
BcoDI GTCTC 2 cut(s) 172, 947
BfaI CTAG 2 cut(s) 977, 985
BfmI CTRYAG 1 cut(s) 433
BfoI RGCGCY 1 cut(s) 84
BisI GCNGC 5 cut(s) 5, 97, 100, 103, 834
BlsI GCNGC 5 cut(s) 6, 98, 101, 104, 835
Bme1390I CCNGG 2 cut(s) 415, 763
BmiI GGNNCC 3 cut(s) 419, 870, 935
BmrFI CCNGG 2 cut(s) 415, 763
BmsI GCATC 6 cut(s) 16, 225, 277, 457, 679, 767
Bpu10I CCTNAGC 1 cut(s) 291
BpuEI CTTGAG 3 cut(s) 371, 440, 637
BpuMI CCSGG 1 cut(s) 415
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 2 cut(s) 100, 762
Bsc4I CCNNNNNNNGG 1 cut(s) 657
BseBI CCWGG 1 cut(s) 763
BseDI CCNNGG 2 cut(s) 100, 762
BseGI GGATG 4 cut(s) 7, 481, 662, 758
BseLI CCNNNNNNNGG 1 cut(s) 657
BseMII CTCAG 4 cut(s) 257, 282, 813, 960
BseSI GKGCMC 2 cut(s) 420, 871
BseXI GCAGC 2 cut(s) 16, 108
BseYI CCCAGC 2 cut(s) 641, 736
BsgI GTGCAG 1 cut(s) 22
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 3 cut(s) 23, 679, 904
BshNI GGYRCC 2 cut(s) 417, 868
BsiSI CCGG 1 cut(s) 414
BslI CCNNNNNNNGG 1 cut(s) 657
BsmAI GTCTC 2 cut(s) 172, 947
BsmI GAATGC 1 cut(s) 791
BsnI GGCC 3 cut(s) 23, 679, 904
Bso31I GGTCTC 1 cut(s) 172
Bsp1286I GDGCHC 2 cut(s) 420, 871
Bsp143I GATC 4 cut(s) 246, 304, 933, 949
BspACI CCGC 7 cut(s) 63, 70, 94, 100, 102, 362, 834
BspANI GGCC 3 cut(s) 23, 679, 904
BspCNI CTCAG 4 cut(s) 256, 283, 812, 961
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 3 cut(s) 419, 870, 935
BspPI GGATC 3 cut(s) 299, 928, 941
BspT107I GGYRCC 2 cut(s) 417, 868
BspTNI GGTCTC 1 cut(s) 172
BssECI CCNNGG 2 cut(s) 100, 762
BssMI GATC 4 cut(s) 246, 304, 933, 949
Bst2UI CCWGG 1 cut(s) 763
Bst4CI ACNGT 2 cut(s) 359, 882
BstC8I GCNNGC 3 cut(s) 74, 136, 819
BstDEI CTNAG 4 cut(s) 243, 291, 799, 969
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 4 cut(s) 7, 481, 662, 758
BstFNI CGCG 1 cut(s) 102
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 4 cut(s) 249, 307, 936, 952
BstMAI GTCTC 2 cut(s) 172, 947
BstMBI GATC 4 cut(s) 246, 304, 933, 949
BstMWI GCNNNNNNNGC 4 cut(s) 78, 102, 144, 676
BstNI CCWGG 1 cut(s) 763
BstSCI CCNGG 2 cut(s) 413, 761
BstSFI CTRYAG 1 cut(s) 433
BstSLI GKGCMC 2 cut(s) 420, 871
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 2 cut(s) 16, 108
BstX2I RGATCY 1 cut(s) 933
BstYI RGATCY 1 cut(s) 933
BsuRI GGCC 3 cut(s) 23, 679, 904
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 4 cut(s) 7, 481, 662, 758
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 3 cut(s) 34, 226, 364
Cac8I GCNNGC 3 cut(s) 74, 136, 819
CfoI GCGC 1 cut(s) 83
Cfr42I CCGCGG 1 cut(s) 103
CseI GACGC 1 cut(s) 696
Csp6I GTAC 2 cut(s) 543, 626
CviQI GTAC 2 cut(s) 543, 626
DdeI CTNAG 4 cut(s) 243, 291, 799, 969
DpnI GATC 4 cut(s) 248, 306, 935, 951
DpnII GATC 4 cut(s) 246, 304, 933, 949
EaeI YGGCCR 2 cut(s) 21, 902
Eco31I GGTCTC 1 cut(s) 172
EcoRI GAATTC 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 761
EcoT22I ATGCAT 2 cut(s) 218, 760
FauI CCCGC 1 cut(s) 101
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 5 cut(s) 5, 97, 100, 103, 834
FokI GGATG 3 cut(s) 488, 669, 745
Fsp4HI GCNGC 5 cut(s) 5, 97, 100, 103, 834
FspBI CTAG 2 cut(s) 977, 985
GlaI GCGC 1 cut(s) 82
GluI GCNGC 5 cut(s) 5, 97, 100, 103, 834
GsaI CCCAGC 2 cut(s) 645, 740
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 3 cut(s) 23, 679, 904
HapII CCGG 1 cut(s) 414
HgaI GACGC 1 cut(s) 696
HhaI GCGC 1 cut(s) 83
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 2 cut(s) 55, 481
HindII GTYRAC 2 cut(s) 55, 481
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 3 cut(s) 460, 586, 981
HpaII CCGG 1 cut(s) 414
Hpy166II GTNNAC 3 cut(s) 55, 481, 610
Hpy188I TCNGA 2 cut(s) 246, 475
Hpy188III TCNNGA 4 cut(s) 388, 457, 464, 501
Hpy8I GTNNAC 3 cut(s) 55, 481, 610
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 3 cut(s) 97, 324, 647
HpyCH4III ACNGT 2 cut(s) 359, 882
HpyCH4V TGCA 9 cut(s) 4, 39, 138, 216, 531, 562, 597, 758, 789
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 102, 144, 676
HpyF3I CTNAG 4 cut(s) 243, 291, 799, 969
HspAI GCGC 1 cut(s) 81
KspI CCGCGG 1 cut(s) 103
Kzo9I GATC 4 cut(s) 246, 304, 933, 949
LmnI GCTCC 1 cut(s) 667
Lsp1109I GCAGC 2 cut(s) 16, 108
LweI GCATC 6 cut(s) 16, 225, 277, 457, 679, 767
MaeI CTAG 2 cut(s) 977, 985
MaeIII GTNAC 1 cut(s) 794
MalI GATC 4 cut(s) 248, 306, 935, 951
MboI GATC 4 cut(s) 246, 304, 933, 949
MboII GAAGA 1 cut(s) 347
MflI RGATCY 1 cut(s) 933
MhlI GDGCHC 2 cut(s) 420, 871
MlsI TGGCCA 2 cut(s) 23, 904
MluCI AATT 9 cut(s) 29, 128, 160, 202, 346, 367, 395, 400, 573
MluNI TGGCCA 2 cut(s) 23, 904
MlyI GAGTC 1 cut(s) 580
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 8 cut(s) 130, 194, 286, 367, 433, 612, 690, 964
Mox20I TGGCCA 2 cut(s) 23, 904
Mph1103I ATGCAT 2 cut(s) 218, 760
MscI TGGCCA 2 cut(s) 23, 904
MseI TTAA 1 cut(s) 695
Msp20I TGGCCA 2 cut(s) 23, 904
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 2 cut(s) 415, 763
Mva1269I GAATGC 1 cut(s) 791
MvaI CCWGG 1 cut(s) 763
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 4 cut(s) 78, 102, 144, 676
NciI CCSGG 1 cut(s) 415
NdeII GATC 4 cut(s) 246, 304, 933, 949
NlaIV GGNNCC 3 cut(s) 419, 870, 935
NsiI ATGCAT 2 cut(s) 218, 760
PctI GAATGC 1 cut(s) 791
PfeI GAWTC 2 cut(s) 460, 981
PkrI GCNGC 5 cut(s) 6, 98, 101, 104, 835
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
Psp6I CCWGG 1 cut(s) 761
PspFI CCCAGC 2 cut(s) 641, 736
PspGI CCWGG 1 cut(s) 761
PspN4I GGNNCC 3 cut(s) 419, 870, 935
PsuI RGATCY 1 cut(s) 933
RsaI GTAC 2 cut(s) 544, 627
RsaNI GTAC 2 cut(s) 543, 626
SacII CCGCGG 1 cut(s) 103
SalI GTCGAC 1 cut(s) 53
SaqAI TTAA 1 cut(s) 695
SatI GCNGC 5 cut(s) 5, 97, 100, 103, 834
Sau3AI GATC 4 cut(s) 246, 304, 933, 949
SchI GAGTC 1 cut(s) 580
ScrFI CCNGG 2 cut(s) 415, 763
SduI GDGCHC 2 cut(s) 420, 871
SetI ASST 9 cut(s) 89, 136, 444, 454, 496, 643, 805, 877, 975
SfaNI GCATC 6 cut(s) 16, 225, 277, 457, 679, 767
SfcI CTRYAG 1 cut(s) 433
Sfr303I CCGCGG 1 cut(s) 103
SgrBI CCGCGG 1 cut(s) 103
SmlI CTYRAG 3 cut(s) 386, 455, 616
SmoI CTYRAG 3 cut(s) 386, 455, 616
Sse9I AATT 9 cut(s) 29, 128, 160, 202, 346, 367, 395, 400, 573
SsiI CCGC 7 cut(s) 63, 70, 94, 100, 102, 362, 834
SspMI CTAG 2 cut(s) 977, 985
StyD4I CCNGG 2 cut(s) 413, 761
TaaI ACNGT 2 cut(s) 359, 882
TaqI TCGA 2 cut(s) 54, 952
TasI AATT 9 cut(s) 29, 128, 160, 202, 346, 367, 395, 400, 573
TatI WGTACW 1 cut(s) 542
TauI GCSGC 3 cut(s) 102, 105, 836
TfiI GAWTC 2 cut(s) 460, 981
Tru1I TTAA 1 cut(s) 695
Tru9I TTAA 1 cut(s) 695
TscAI CASTG 3 cut(s) 41, 226, 364
TseI GCWGC 2 cut(s) 4, 96
TspGWI ACGGA 2 cut(s) 860, 926
TspRI CASTG 3 cut(s) 41, 226, 364
XapI RAATTY 3 cut(s) 29, 367, 400
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 2 cut(s) 977, 985
Zsp2I ATGCAT 2 cut(s) 218, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.