Rh4AG087100

Endonuclease I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003467
Physical Location & Seq
Reverse (-)
18246396 .. 18253193
6798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG087100.1

Sequence Viewer

Length: 897 bp
ATGCAGTCTTCTCCAGTAATGGCCACCAAATTCCATTGCACAAAACCCGTCGTCGTCTGTAAGCAGAGACCCAGCTTTTTCCGGGGCCCCGAGAACTTCAAACTCAAGCCTTTTAATCGAAACCCAGATCATATTTTGGTCAGTCCAATCACCTCCACCATATCTACAAGGGGCATATGGGATTTCACAAAGTGCATCAATATCTCATGGAGATGGGTTTCTCAGATTCTCTCTGTTTACTTGCTTTTTCTCAATATTGTTGCTGATGCTCAGGCCTATGATTACCCATCTCCTTCTTCTTCTTCTTCTGTTTATGCTTGTGAAGATGTTAACAATTACTATGCCAATGTGGAGCATTTAGAGGGTAAATCGCTCAAGAAGAAACTGAATTCCATCATTGGCAAGCATCAATCTTTATCTTACAGAGAGGTGTGGAATGCTCTCAAGATTCTAGATGCTTCGGATGTTGACAACCCAGAAGCTTCATCAGGGATAGTTGAAATTTACTCCGTGAAGGTTGTACCAAAGTCATTAGCTGGAAAGCCTGAAGGATGGAATAGAGAGCACTTATGGCCTCGTTCTTATGGTTTGAAAAATGGTCCATCCCTGACTGATGTACACAACATTCGCCCTGCAGATGTGAATGTCAATTCCTCCAGGGGGAACAAATACTACGGAGAATGCATTGTTAATTCGGCTAAATGTTTGAAGCCAGCAAATAAAGAAGCTGCTTTGGACACAGAAACTGACAAGGATAGATGGGCACCACCTAAACAGCATAGAGGAGATATTGCTAGGGCACTGATGTACATGGCGGTATGTTATGGATATCGTCAACCAGATGGAGGCCCAGTTCTTCACCTTTCAGATTCTCCAAAAATTTGTAAGTTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endonuclease_1

Protein Analysis

298

Amino Acids

33.48

Weight (kDa)

9.18

Isoelectric Point (pI)

53.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_1 PF04231 122 - 277 5.4e-25 Endonuclease I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 763
AciI CCGC 1 cut(s) 815
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 4 cut(s) 29, 388, 501, 879
AcuI CTGAAG 1 cut(s) 567
AdeI CACNNNGTG 1 cut(s) 192
AfaI GTAC 3 cut(s) 522, 618, 809
AfiI CCNNNNNNNGG 2 cut(s) 660, 845
AgsI TTSAA 4 cut(s) 100, 500, 592, 709
AjnI CCWGG 1 cut(s) 656
AloI GAACNNNNNNTCC 2 cut(s) 837, 869
AluBI AGCT 4 cut(s) 75, 482, 536, 728
AluI AGCT 4 cut(s) 75, 482, 536, 728
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 1 cut(s) 61
AlwNI CAGNNNCTG 1 cut(s) 746
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 5 cut(s) 21, 85, 273, 572, 847
ApaI GGGCCC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 728
ApoI RAATTY 4 cut(s) 29, 388, 501, 879
AspS9I GGNCC 4 cut(s) 85, 86, 599, 848
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 2 cut(s) 142, 851
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 1 cut(s) 599
BaeGI GKGCMC 3 cut(s) 89, 766, 802
BalI TGGCCA 1 cut(s) 23
BanI GGYRCC 1 cut(s) 763
BanII GRGCYC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 567
BbvI GCAGC 1 cut(s) 715
BccI CCATC 7 cut(s) 207, 295, 401, 546, 610, 753, 836
BciT130I CCWGG 1 cut(s) 658
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 2 cut(s) 452, 795
BfmI CTRYAG 1 cut(s) 633
BisI GCNGC 1 cut(s) 729
BlsI GCNGC 1 cut(s) 730
Bme1390I CCNGG 2 cut(s) 83, 658
Bme18I GGWCC 1 cut(s) 599
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 4 cut(s) 85, 86, 599, 848
BmiI GGNNCC 4 cut(s) 86, 87, 88, 765
BmrFI CCNGG 2 cut(s) 83, 658
BmrI ACTGGG 1 cut(s) 845
BmsI GCATC 4 cut(s) 204, 256, 415, 445
BmuI ACTGGG 1 cut(s) 845
BpmI CTGGAG 1 cut(s) 640
Bpu10I CCTNAGC 1 cut(s) 270
BpuEI CTTGAG 3 cut(s) 89, 359, 428
BpuMI CCSGG 1 cut(s) 83
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 2 cut(s) 82, 657
BsaXI ACNNNNNCTCC 2 cut(s) 837, 867
Bsc4I CCNNNNNNNGG 2 cut(s) 660, 845
Bse1I ACTGG 2 cut(s) 14, 851
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 658
BseDI CCNNGG 2 cut(s) 82, 657
BseGI GGATG 3 cut(s) 469, 557, 602
BseLI CCNNNNNNNGG 2 cut(s) 660, 845
BseMI GCAATG 1 cut(s) 34
BseMII CTCAG 2 cut(s) 236, 284
BseNI ACTGG 2 cut(s) 14, 851
BseRI GAGGAG 1 cut(s) 798
BseSI GKGCMC 3 cut(s) 89, 766, 802
BseXI GCAGC 1 cut(s) 715
BseYI CCCAGC 1 cut(s) 71
BshFI GGCC 5 cut(s) 23, 87, 275, 574, 849
BshNI GGYRCC 1 cut(s) 763
BsiHKAI GWGCWC 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 1 cut(s) 82
BslI CCNNNNNNNGG 2 cut(s) 660, 845
BsmAI GTCTC 1 cut(s) 61
BsmI GAATGC 2 cut(s) 442, 686
BsnI GGCC 5 cut(s) 23, 87, 275, 574, 849
Bso31I GGTCTC 1 cut(s) 61
BsoBI CYCGRG 1 cut(s) 89
Bsp120I GGGCCC 1 cut(s) 85
Bsp1286I GDGCHC 4 cut(s) 89, 567, 766, 802
Bsp1407I TGTACA 2 cut(s) 616, 807
Bsp143I GATC 1 cut(s) 127
BspACI CCGC 1 cut(s) 815
BspANI GGCC 5 cut(s) 23, 87, 275, 574, 849
BspCNI CTCAG 2 cut(s) 235, 283
BspLI GGNNCC 4 cut(s) 86, 87, 88, 765
BspMAI CTGCAG 1 cut(s) 637
BspT107I GGYRCC 1 cut(s) 763
BspTNI GGTCTC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 34
BsrGI TGTACA 2 cut(s) 616, 807
BsrI ACTGG 2 cut(s) 14, 851
BssECI CCNNGG 2 cut(s) 82, 657
BssMI GATC 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 658
BstAUI TGTACA 2 cut(s) 616, 807
BstC8I GCNNGC 2 cut(s) 404, 714
BstDEI CTNAG 2 cut(s) 222, 270
BstF5I GGATG 3 cut(s) 469, 557, 602
BstKTI GATC 1 cut(s) 130
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 571
BstNI CCWGG 1 cut(s) 658
BstSCI CCNGG 2 cut(s) 81, 656
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 3 cut(s) 89, 766, 802
BstV1I GCAGC 1 cut(s) 715
BsuRI GGCC 5 cut(s) 23, 87, 275, 574, 849
BtsCI GGATG 3 cut(s) 469, 557, 602
BtsIMutI CAGTG 1 cut(s) 800
Cac8I GCNNGC 2 cut(s) 404, 714
CaiI CAGNNNCTG 1 cut(s) 746
Cfr13I GGNCC 4 cut(s) 85, 86, 599, 848
Csp6I GTAC 3 cut(s) 521, 617, 808
CviAII CATG 2 cut(s) 207, 811
CviQI GTAC 3 cut(s) 521, 617, 808
DdeI CTNAG 2 cut(s) 222, 270
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
DraIII CACNNNGTG 1 cut(s) 192
EaeI YGGCCR 1 cut(s) 21
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 830
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 89
EcoO109I RGGNCCY 2 cut(s) 85, 86
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 656
EcoRV GATATC 1 cut(s) 830
EcoT22I ATGCAT 1 cut(s) 686
EcoT38I GRGCYC 1 cut(s) 89
FaeI CATG 2 cut(s) 210, 814
FatI CATG 2 cut(s) 206, 810
FauNDI CATATG 1 cut(s) 176
Fnu4HI GCNGC 1 cut(s) 729
FokI GGATG 3 cut(s) 476, 564, 589
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 1 cut(s) 729
FspBI CTAG 2 cut(s) 452, 795
GluI GCNGC 1 cut(s) 729
GsaI CCCAGC 1 cut(s) 75
GsuI CTGGAG 1 cut(s) 640
HaeIII GGCC 5 cut(s) 23, 87, 275, 574, 849
HapII CCGG 1 cut(s) 82
Hin1II CATG 2 cut(s) 210, 814
HincII GTYRAC 3 cut(s) 331, 469, 836
HindII GTYRAC 3 cut(s) 331, 469, 836
HindIII AAGCTT 1 cut(s) 480
HinfI GANTC 3 cut(s) 226, 448, 869
HpaI GTTAAC 1 cut(s) 331
HpaII CCGG 1 cut(s) 82
HphI GGTGA 2 cut(s) 142, 851
Hpy166II GTNNAC 5 cut(s) 238, 331, 469, 619, 836
Hpy188I TCNGA 3 cut(s) 225, 463, 868
Hpy188III TCNNGA 3 cut(s) 376, 445, 452
Hpy8I GTNNAC 5 cut(s) 238, 331, 469, 619, 836
Hpy99I CGWCG 2 cut(s) 53, 56
HpyAV CCTTC 3 cut(s) 303, 508, 542
HpyCH4V TGCA 5 cut(s) 4, 39, 195, 635, 684
HpyF10VI GCNNNNNNNGC 1 cut(s) 571
HpyF3I CTNAG 2 cut(s) 222, 270
Hsp92II CATG 2 cut(s) 210, 814
KspAI GTTAAC 1 cut(s) 331
Kzo9I GATC 1 cut(s) 127
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 1 cut(s) 715
LweI GCATC 4 cut(s) 204, 256, 415, 445
MaeI CTAG 2 cut(s) 452, 795
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MboII GAAGA 7 cut(s) 288, 291, 294, 297, 335, 391, 848
MhlI GDGCHC 4 cut(s) 89, 567, 766, 802
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 7 cut(s) 29, 334, 388, 501, 649, 691, 879
MluNI TGGCCA 1 cut(s) 23
MnlI CCTC 7 cut(s) 163, 355, 421, 585, 664, 776, 839
Mox20I TGGCCA 1 cut(s) 23
Mph1103I ATGCAT 1 cut(s) 686
MscI TGGCCA 1 cut(s) 23
MseI TTAA 4 cut(s) 114, 330, 690, 890
MslI CAYNNNNRTG 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 23
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 2 cut(s) 83, 658
Mva1269I GAATGC 2 cut(s) 442, 686
MvaI CCWGG 1 cut(s) 658
MwoI GCNNNNNNNGC 1 cut(s) 571
NciI CCSGG 1 cut(s) 83
NdeI CATATG 1 cut(s) 176
NdeII GATC 1 cut(s) 127
NlaIII CATG 2 cut(s) 210, 814
NlaIV GGNNCC 4 cut(s) 86, 87, 88, 765
NsiI ATGCAT 1 cut(s) 686
PceI AGGCCT 1 cut(s) 275
PctI GAATGC 2 cut(s) 442, 686
PfeI GAWTC 3 cut(s) 226, 448, 869
PkrI GCNGC 1 cut(s) 730
Psp6I CCWGG 1 cut(s) 656
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 1 cut(s) 656
PspN4I GGNNCC 4 cut(s) 86, 87, 88, 765
PspOMI GGGCCC 1 cut(s) 85
PspPI GGNCC 4 cut(s) 85, 86, 599, 848
PsrI GAACNNNNNNTAC 2 cut(s) 656, 688
PstI CTGCAG 1 cut(s) 637
PstNI CAGNNNCTG 1 cut(s) 746
RsaI GTAC 3 cut(s) 522, 618, 809
RsaNI GTAC 3 cut(s) 521, 617, 808
RseI CAYNNNNRTG 1 cut(s) 211
SaqAI TTAA 4 cut(s) 114, 330, 690, 890
SatI GCNGC 1 cut(s) 729
Sau3AI GATC 1 cut(s) 127
Sau96I GGNCC 4 cut(s) 85, 86, 599, 848
ScrFI CCNGG 2 cut(s) 83, 658
SduI GDGCHC 4 cut(s) 89, 567, 766, 802
SetI ASST 9 cut(s) 77, 155, 432, 484, 519, 538, 730, 772, 864
SfaNI GCATC 4 cut(s) 204, 256, 415, 445
SfcI CTRYAG 1 cut(s) 633
SinI GGWCC 1 cut(s) 599
SmiMI CAYNNNNRTG 1 cut(s) 211
SmlI CTYRAG 3 cut(s) 104, 374, 443
SmoI CTYRAG 3 cut(s) 104, 374, 443
Sse9I AATT 7 cut(s) 29, 334, 388, 501, 649, 691, 879
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 1 cut(s) 815
SspI AATATT 1 cut(s) 256
SspMI CTAG 2 cut(s) 452, 795
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 2 cut(s) 81, 656
TaqI TCGA 1 cut(s) 118
TasI AATT 7 cut(s) 29, 334, 388, 501, 649, 691, 879
TatI WGTACW 2 cut(s) 616, 807
TfiI GAWTC 3 cut(s) 226, 448, 869
Tru1I TTAA 4 cut(s) 114, 330, 690, 890
Tru9I TTAA 4 cut(s) 114, 330, 690, 890
TscAI CASTG 1 cut(s) 807
TseI GCWGC 1 cut(s) 728
TspDTI ATGAA 1 cut(s) 474
TspGWI ACGGA 2 cut(s) 499, 690
TspRI CASTG 1 cut(s) 807
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 4 cut(s) 29, 388, 501, 879
XbaI TCTAGA 1 cut(s) 451
XspI CTAG 2 cut(s) 452, 795
Zsp2I ATGCAT 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.