Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0013028

Physical Location & Seq

Genomic Coordinates Forward (+)

11932458 .. 11937196

Gene Structure 4739 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g09820.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3165 bp

ATGAGAGCAGGCCTGAGCACGATCCAGCAAACCTTAACGCCGGAGGCGGCGAGCGTCTTGAACCACTCGATCGCCGAAGCGGGTCGTCGCAACCACGGCCAAACGACGCCGCTTCATGTTGCGGCAACTCTTCTTTCCTCCCCCACCGGCTTCCTCCGCCAAGCATGCATCAAATCTCATCCCAATTCATCTCACCCTCTCCAGTGCAGAGCGCTCGAGCTCTGCTTCAGTGTCGCCCTGGAGCGGTTGCCCACGGCGCAGAACATGAGCCCCGGGATGGAGCCGCCCATCTCCAATGCCCTCATGGCCGCCTTAAAGCGGGCCCAGGCCCACCAGCGCAGAGGCTGCCCGGAGCAGCAGCAGCAGCCGCTCTTGGCCGTCAAAGTCGAACTCGAGCAGCTCATAATCTCGATTCTCGATGACCCCAGTGTTAGTCGGGTCATGCGGGAGGCCAGTTTCTCTAGTCCGGCAGTTAAAGCGACCATCGAACAGTCTCTTAACTCTTCATCAGCGGCGGCGGCAGCGACCTCTACCGTCGCCGCGAATTCGTCCCCAATTGGATTGGGGTTCCGGCCTGCGGGACCGCCCGCCGGTCGGAACATGTATTTAAATCCCCGGCTGCAGGGAGCAGCAGGGCAATCGGGGCAAAACCGAGCAGAGGAAGTTAAAAAAGTTGCTGATATTTTATCAAGGGGGAAGAAGAGGAATCCGGTATTGGTCGGCGATTCGGAGCCGGAGGCTGTGACGAAAGAGCTGTTCCGGCGGATTCAGAGCGCGGAATTGGGAGAAGAGCAGTTAAAGAATGTGGAGATCATTCATTTGGAGAAGGAATTTTCTTCGGAGAGGGGGCAGATATTGGGGAAGATGAAAGAGTTGATGTCTTTAGTTGAGACGCGGATGACCAGTTCGAATGGGAGAGGGATGATTCTTGATTTGGGGGATTTGAAATGGTTGGTGGGGCAGCCGGTTAGCTTGGGAACGGTTGGTCCCGGCCCCGGTGGCCAACAGGTGGTTTCGGAGGCGGGACGGGCGGCGGTGGCGGAAATGGGGAAGGTGTTGGGGAGGTTCGGTGAGGGCGGTGTTAATGGCGGTGGCCGGCTTTGGTTGATAGGGACGGCTACTTGTGAGACTTATTTGAGGTGCCAAGTCTATCACCCTTTAATGGAAACTGATTGGGATCTACAGGCAGTGCCGATTGCTGCCAGGACACCTTTTTCCGGATTGTTTCCCAGGATGGGGACAACGAACGGGATCCTTAGTAGCTCAGTTGAATCTTTGTCTCCATTGAAAGGCTTTCCGACTGCTCAACAGAGGCTTGTGGCTGAAAACTTGGATCCTGTTCGAAGAACAAGCTGTTGCCCGCAATGTACAGAAACTTGTGAGCAAGAAGTATCAAAATTGGTTGCAAAGGAGTATGAGAAATCATATTCTGAATCAAAATCGGAAGCAGCTCAACCAGCACTACCACAGTGGTTGCAGAATGCTAAAGCCCAGGACAATAATGTCAAAATGTCAGATCAGTTGCAGACCAAGAACCAAGATCAGACCCTGAATGAGAAGACTCAACAATTACGAAAGGAATGGAAAGATACATGCATGCGCCTTCATCCTAATTTTCATCAGCATAGTTTCAGCTCAGAAAGAATTGCTCCACAACCTCTCTCAATCACCAGCATGTACAATATGAACTTGCTTGGTCGCCAATCTTTCCAACCGAAATCGCAACCAAACAAAAGTTTTGGGGCTCTGCAATTGAACACAAATCTACAGACCAGCCAATCATCGGAACGTGCAGCAGTTTCGCATCCACGAAGCCCTGTAAGGACGGACCTTGTGCTTGGGCAAAAAGAAGTTACTGAAACCACCACTCCTGAGCAAATGCACAAGGAGCACGTCAAAGATTTCATGGGCTGCATGCCTTCTGAACCACTGAACAAGTTACTTGAAAGGCAAACAGATGATAAGCAATTGTGCCAATTAGATGCTGATTCATTCAAAAAGCTATACAAGGGTTTGATGGAGGTCTGGTGGCAGCAGGAAGCAGCAGCCGCTGTCGCTTCAACTATAACCAATTGTAAGTTGGGTAATGGAAAGAGGCGTGGTGCCGGATCAAGGGGAGACATGTGGTTGTTGTTTATGGGTCCGGACAGTGTTGGCAAGAAGAAGATGGCATCAGCTCTTTCAGAAATGGTATCTGGATCCACCCCGGTGATGATCTCTCTTAACAATAAACGTGGTAGTTGGGATTCAGACATGAGTTTCCGTGGAAAAACGGTGGTGGACCGAATAGCTGAGGCTGTGAGGAGGAACCCCTTTTCTGTAATCATGCTTGAGGACGTTAATGAAGCAGATATGATAGTTCGCGGGAGCATAAAACGAGCAATGGAGAGAGGTCGTCTTGCCGACTCTTATGGCCGTGAAATCAGTCTTGGAAATGTCATCTTTATTCTCACTGCAAATTGGTTGCCAGAAAATCTGAAACACTTATCAAAGGTCGATTCACTTGAAGAAAAGCTTGCTTGTATAGCTAGGAGTGGTTGGCAGTTAAAGCTATCCATTTGTGGAAGAAGTACAAAACGGCGAGCAACATGGTTACAGAGCAATGAAGACAGAGCCACAAAGCCTAGGAAAGATGCGAGTTCTGGACTAGGATTCGATCTAAATGAAGCTGCTGATGTTGGGGACGACAGAACAGATGGTTCACTTAATTCAAGTGATCTAACAGTTGATCATGAAGATGAGAACCGCCTCAATAACAGGTCATTGCTCACAAGTACACCTTCATCAGCACCAAGGGAATTGCTTGATTCTGTTGACCATGCCATTGTGTTCAAGCCAGTGGACTTCAACCCTATTCAGAAAAACATCACAAACTCAATTACAAGAAGATTTTCAATGATCATTGGCGACAGAGTTCCCTTGGAATTGCAAGATGATACTGTTGAAAAGATCCTTAGTGGGATATGGCTAGGCAAGACCGGATTAGATGAATGGATAGAGAAGATTCTAGTCCCAAGCCTCCAACAGCTGAAGTCCAGCCTAGGTGTGACACTCGACGAGTCAATGGTCGTTAGGCTCGAAGCTGATGGAGATTCGGGTTGCCGGAGACAAGGAGATTGGCTGCCGAGTAGCATCAATGTGGTGGCTGACGGATTAAGGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007275 GO:0007623 GO:0008150 GO:0009628 GO:0009719 GO:0009725 GO:0009791 GO:0009845 GO:0010033 GO:0014070 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0048511 GO:0048856 GO:0050896 GO:0080167 GO:0090351 GO:1901700 GO:1902347

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_2 Clp_N

Protein Analysis

1055

Amino Acids

115.65

Weight (kDa)

8.41

Isoelectric Point (pI)

51.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	12 - 113	1.1e-06	Clp repeat (R) N-terminal domain
NBD_SMAX1	PF23569	218 - 401	4.7e-29	SMAX1 nucleotide binding domain
AAA_2	PF07724	710 - 828	3.3e-09	AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1	PF26587	948 - 1031	1.4e-24	SMAX1-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57710
fragaria_vesca	FvH4_4g09820
malus_domestica	MD13G1261900.v1.1 MD16G1264200.v1.1
prunus_persica	Prupe.1G095200_v2.0.a1 Prupe.1G095200_v2.0.a1
pyrus_communis	pycom13g24720 pycom16g23290
rosa_chinensis	RchiOBHm_Chr4g0404601
rosa_laevigata	RLG00000008920
rosa_multiflora	Rmu_sc0002699.1_g000006
rosa_roxburghii	Rroxscaffold_5G00348750
rosa_rugosa	Rorug04G0047700
rosa_samantha	Rh4AG123800 Rh4BG117200 Rh4CG131900 Rh4DG116700
rosa_wichuraiana	Rw4G010030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1502
AccB1I	GGYRCC	2 cut(s)	1140, 2102
AccB7I	CCANNNNNTGG	1 cut(s)	1009
AccBSI	CCGCTC	2 cut(s)	244, 370
AccII	CGCG	4 cut(s)	542, 776, 895, 2364
AccIII	TCCGGA	2 cut(s)	1217, 2143
AcoI	YGGCCR	6 cut(s)	97, 306, 375, 1000, 1093, 2413
AcsI	RAATTY	2 cut(s)	544, 830
AcuI	CTGAAG	2 cut(s)	211, 3050
AcyI	GRCGYC	1 cut(s)	107
AfaI	GTAC	4 cut(s)	1369, 1679, 2572, 2776
AfeI	AGCGCT	1 cut(s)	213
AflIII	ACRYGT	2 cut(s)	600, 2121
AjiI	CACGTC	1 cut(s)	1894
AjnI	CCWGG	5 cut(s)	237, 324, 1202, 1229, 1491
AjuI	GAANNNNNNNTTGG	4 cut(s)	698, 730, 2412, 2444
AleI	CACNNNNGTG	1 cut(s)	2207
AloI	GAACNNNNNNTCC	4 cut(s)	889, 921, 970, 1002
Alw21I	GWGCWC	3 cut(s)	20, 222, 1893
Alw26I	GTCTC	6 cut(s)	498, 884, 1121, 1284, 2112, 3100
AlwNI	CAGNNNCTG	3 cut(s)	345, 1549, 2051
Ama87I	CYCGRG	3 cut(s)	215, 272, 392
Aor13HI	TCCGGA	2 cut(s)	1217, 2143
Aor51HI	AGCGCT	1 cut(s)	213
ApaI	GGGCCC	1 cut(s)	325
ApoI	RAATTY	2 cut(s)	544, 830
Asp700I	GAANNNNTTC	1 cut(s)	2890
AspA2I	CCTAGG	2 cut(s)	2624, 3040
AspLEI	GCGC	5 cut(s)	214, 259, 339, 776, 1602
AspS9I	GGNCC	9 cut(s)	321, 322, 328, 581, 986, 992, 1828, 2141, 2281
AsuC2I	CCSGG	7 cut(s)	273, 274, 350, 616, 990, 996, 2207
AsuHPI	GGTGA	5 cut(s)	185, 1082, 1145, 1660, 2221
AsuII	TTCGAA	2 cut(s)	908, 1342
AvaI	CYCGRG	3 cut(s)	215, 272, 392
AvaII	GGWCC	5 cut(s)	581, 986, 1828, 2141, 2281
AvrII	CCTAGG	2 cut(s)	2624, 3040
BaeGI	GKGCMC	1 cut(s)	325
BalI	TGGCCA	1 cut(s)	1002
BamHI	GGATCC	3 cut(s)	1251, 1333, 2198
BanI	GGYRCC	2 cut(s)	1140, 2102
BanII	GRGCYC	4 cut(s)	222, 272, 325, 1747
BbsI	GAAGAC	2 cut(s)	1565, 2613
Bbv12I	GWGCWC	3 cut(s)	20, 222, 1893
BbvCI	CCTCAGC	1 cut(s)	2292
BccI	CCATC	8 cut(s)	271, 296, 491, 1228, 2011, 2161, 2690, 3080
BceAI	ACGGC	6 cut(s)	112, 270, 362, 1131, 2400, 2594
BcgI	CGANNNNNNTGC	6 cut(s)	196, 230, 621, 655, 1782, 1816
BciT130I	CCWGG	5 cut(s)	239, 326, 1204, 1231, 1493
BclI	TGATCA	2 cut(s)	2728, 2898
BcnI	CCSGG	7 cut(s)	273, 274, 350, 616, 990, 996, 2207
BcoDI	GTCTC	6 cut(s)	498, 884, 1121, 1284, 2112, 3100
BfaI	CTAG	7 cut(s)	462, 2529, 2625, 2648, 2969, 3008, 3041
BfmI	CTRYAG	3 cut(s)	620, 1181, 1766
BfoI	RGCGCY	1 cut(s)	215
BglI	GCCNNNNNGGC	2 cut(s)	305, 999
BlnI	CCTAGG	2 cut(s)	2624, 3040
Bme18I	GGWCC	5 cut(s)	581, 986, 1828, 2141, 2281
BmeT110I	CYCGRG	3 cut(s)	215, 272, 392
BmgBI	CACGTC	1 cut(s)	1894
BmgT120I	GGNCC	9 cut(s)	321, 322, 328, 581, 986, 992, 1828, 2141, 2281
BmrI	ACTGGG	1 cut(s)	420
BmsI	GCATC	6 cut(s)	177, 1813, 1972, 2180, 2623, 3141
BmuI	ACTGGG	1 cut(s)	420
BpiI	GAAGAC	2 cut(s)	1565, 2613
BpmI	CTGGAG	2 cut(s)	185, 260
Bpu10I	CCTNAGC	3 cut(s)	14, 1872, 2292
Bpu14I	TTCGAA	2 cut(s)	908, 1342
BpuEI	CTTGAG	1 cut(s)	2351
BpuMI	CCSGG	7 cut(s)	273, 274, 350, 616, 990, 996, 2207
BsaBI	GATNNNNATC	1 cut(s)	1176
BsaHI	GRCGYC	1 cut(s)	107
BsaWI	WCCGGW	4 cut(s)	709, 1217, 2143, 2978
BsaXI	ACNNNNNCTCC	6 cut(s)	1054, 1084, 1852, 1882, 2012, 2042
Bse118I	RCCGGY	4 cut(s)	146, 590, 964, 1095
Bse1I	ACTGG	5 cut(s)	202, 426, 453, 903, 2837
Bse3DI	GCAATG	4 cut(s)	1370, 2388, 2608, 2762
Bse8I	GATNNNNATC	1 cut(s)	1176
BseAI	TCCGGA	2 cut(s)	1217, 2143
BseBI	CCWGG	5 cut(s)	239, 326, 1204, 1231, 1493
BseGI	GGATG	7 cut(s)	178, 282, 903, 927, 1239, 1606, 1804
BseJI	GATNNNNATC	1 cut(s)	1176
BseMI	GCAATG	4 cut(s)	1370, 2388, 2608, 2762
BseMII	CTCAG	5 cut(s)	5, 1278, 1650, 1863, 2283
BseNI	ACTGG	5 cut(s)	202, 426, 453, 903, 2837
BseRI	GAGGAG	1 cut(s)	2317
BseSI	GKGCMC	1 cut(s)	325
BsgI	GTGCAG	2 cut(s)	226, 1812
Bsh1236I	CGCG	4 cut(s)	542, 776, 895, 2364
Bsh1285I	CGRYCG	2 cut(s)	72, 595
BshNI	GGYRCC	2 cut(s)	1140, 2102
BsiEI	CGRYCG	2 cut(s)	72, 595
BsiHKAI	GWGCWC	3 cut(s)	20, 222, 1893
BsiHKCI	CYCGRG	3 cut(s)	215, 272, 392
BslFI	GGGAC	8 cut(s)	535, 594, 972, 1038, 1126, 1252, 2696, 2996
BsmAI	GTCTC	6 cut(s)	498, 884, 1121, 1284, 2112, 3100
BsmBI	CGTCTC	1 cut(s)	884
BsmFI	GGGAC	8 cut(s)	535, 594, 972, 1038, 1126, 1252, 2696, 2996
BsmI	GAATGC	1 cut(s)	1486
BsoBI	CYCGRG	3 cut(s)	215, 272, 392
Bsp119I	TTCGAA	2 cut(s)	908, 1342
Bsp120I	GGGCCC	1 cut(s)	321
Bsp1286I	GDGCHC	6 cut(s)	20, 222, 272, 325, 1747, 1893
Bsp13I	TCCGGA	2 cut(s)	1217, 2143
Bsp1407I	TGTACA	2 cut(s)	1367, 1677
BspCNI	CTCAG	5 cut(s)	6, 1277, 1649, 1864, 2284
BspEI	TCCGGA	2 cut(s)	1217, 2143
BspFNI	CGCG	4 cut(s)	542, 776, 895, 2364
BspHI	TCATGA	1 cut(s)	2731
BspMAI	CTGCAG	1 cut(s)	624
BspQI	GCTCTTC	1 cut(s)	783
BspT104I	TTCGAA	2 cut(s)	908, 1342
BspT107I	GGYRCC	2 cut(s)	1140, 2102
BsrBI	CCGCTC	2 cut(s)	244, 370
BsrDI	GCAATG	4 cut(s)	1370, 2388, 2608, 2762
BsrFI	RCCGGY	4 cut(s)	146, 590, 964, 1095
BsrGI	TGTACA	2 cut(s)	1367, 1677
BsrI	ACTGG	5 cut(s)	202, 426, 453, 903, 2837
BssAI	RCCGGY	4 cut(s)	146, 590, 964, 1095
BssNI	GRCGYC	1 cut(s)	107
BssT1I	CCWWGG	4 cut(s)	2624, 2792, 2919, 3040
Bst2UI	CCWGG	5 cut(s)	239, 326, 1204, 1231, 1493
Bst4CI	ACNGT	8 cut(s)	492, 535, 982, 1470, 2150, 2275, 2725, 2941
Bst6I	CTCTTC	4 cut(s)	135, 508, 695, 783
BstACI	GRCGYC	1 cut(s)	107
BstAPI	GCANNNNNTGC	1 cut(s)	345
BstAUI	TGTACA	2 cut(s)	1367, 1677
BstBI	TTCGAA	2 cut(s)	908, 1342
BstDEI	CTNAG	7 cut(s)	14, 1256, 1264, 1636, 1872, 2292, 2954
BstDSI	CCRYGG	3 cut(s)	94, 252, 2263
BstF5I	GGATG	7 cut(s)	178, 282, 903, 927, 1239, 1606, 1804
BstFNI	CGCG	4 cut(s)	542, 776, 895, 2364
BstH2I	RGCGCY	1 cut(s)	215
BstHHI	GCGC	5 cut(s)	214, 259, 339, 776, 1602
BstMAI	GTCTC	6 cut(s)	498, 884, 1121, 1284, 2112, 3100
BstMCI	CGRYCG	2 cut(s)	72, 595
BstNI	CCWGG	5 cut(s)	239, 326, 1204, 1231, 1493
BstNSI	RCATGY	7 cut(s)	168, 604, 1596, 1600, 1678, 1918, 2125
BstSFI	CTRYAG	3 cut(s)	620, 1181, 1766
BstSLI	GKGCMC	1 cut(s)	325
BstUI	CGCG	4 cut(s)	542, 776, 895, 2364
BstV2I	GAAGAC	2 cut(s)	1565, 2613
BstX2I	RGATCY	5 cut(s)	1177, 1251, 1333, 2198, 2949
BstYI	RGATCY	5 cut(s)	1177, 1251, 1333, 2198, 2949
BtgI	CCRYGG	3 cut(s)	94, 252, 2263
BtrI	CACGTC	1 cut(s)	1894
BtsCI	GGATG	7 cut(s)	178, 282, 903, 927, 1239, 1606, 1804
BtsI	GCAGTG	2 cut(s)	1194, 2451
BtsIMutI	CAGTG	9 cut(s)	209, 235, 433, 1194, 1475, 1928, 2155, 2451, 2844
CaiI	CAGNNNCTG	3 cut(s)	345, 1549, 2051
CciI	TCATGA	1 cut(s)	2731
CfoI	GCGC	5 cut(s)	214, 259, 339, 776, 1602
Cfr10I	RCCGGY	4 cut(s)	146, 590, 964, 1095
Cfr13I	GGNCC	9 cut(s)	321, 322, 328, 581, 986, 992, 1828, 2141, 2281
Cfr9I	CCCGGG	1 cut(s)	272
CseI	GACGC	3 cut(s)	43, 115, 901
Csp6I	GTAC	4 cut(s)	1368, 1678, 2571, 2775
CspCI	CAANNNNNGTGG	4 cut(s)	1455, 1490, 2215, 2250
CviQI	GTAC	4 cut(s)	1368, 1678, 2571, 2775
DdeI	CTNAG	7 cut(s)	14, 1256, 1264, 1636, 1872, 2292, 2954
DraI	TTTAAA	1 cut(s)	609
DrdI	GACNNNNNNGTC	1 cut(s)	1502
DseDI	GACNNNNNNGTC	1 cut(s)	1502
EaeI	YGGCCR	6 cut(s)	97, 306, 375, 1000, 1093, 2413
Eam1104I	CTCTTC	4 cut(s)	135, 508, 695, 783
EarI	CTCTTC	4 cut(s)	135, 508, 695, 783
EciI	GGCGGA	3 cut(s)	146, 778, 1055
Ecl136II	GAGCTC	1 cut(s)	220
Eco130I	CCWWGG	4 cut(s)	2624, 2792, 2919, 3040
Eco147I	AGGCCT	1 cut(s)	12
Eco24I	GRGCYC	4 cut(s)	222, 272, 325, 1747
Eco47I	GGWCC	5 cut(s)	581, 986, 1828, 2141, 2281
Eco47III	AGCGCT	1 cut(s)	213
Eco53kI	GAGCTC	1 cut(s)	220
Eco57I	CTGAAG	2 cut(s)	211, 3050
Eco88I	CYCGRG	3 cut(s)	215, 272, 392
EcoICRI	GAGCTC	1 cut(s)	220
EcoRI	GAATTC	1 cut(s)	544
EcoRII	CCWGG	5 cut(s)	237, 324, 1202, 1229, 1491
EcoT14I	CCWWGG	4 cut(s)	2624, 2792, 2919, 3040
EcoT22I	ATGCAT	2 cut(s)	170, 1598
EcoT38I	GRGCYC	4 cut(s)	222, 272, 325, 1747
ErhI	CCWWGG	4 cut(s)	2624, 2792, 2919, 3040
Esp3I	CGTCTC	1 cut(s)	884
FalI	AAGNNNNNCTT	4 cut(s)	2499, 2531, 2764, 2796
FaqI	GGGAC	8 cut(s)	535, 594, 972, 1038, 1126, 1252, 2696, 2996
FauI	CCCGC	8 cut(s)	73, 312, 438, 571, 595, 1015, 1368, 2357
FbaI	TGATCA	2 cut(s)	2728, 2898
FokI	GGATG	7 cut(s)	165, 289, 910, 934, 1246, 1593, 1791
FriOI	GRGCYC	4 cut(s)	222, 272, 325, 1747
FspBI	CTAG	7 cut(s)	462, 2529, 2625, 2648, 2969, 3008, 3041
GlaI	GCGC	5 cut(s)	213, 258, 338, 775, 1601
GsuI	CTGGAG	2 cut(s)	185, 260
HaeII	RGCGCY	1 cut(s)	215
HgaI	GACGC	3 cut(s)	43, 115, 901
HhaI	GCGC	5 cut(s)	214, 259, 339, 776, 1602
Hin1I	GRCGYC	1 cut(s)	107
Hin6I	GCGC	5 cut(s)	212, 257, 337, 774, 1600
HinP1I	GCGC	5 cut(s)	212, 257, 337, 774, 1600
HincII	GTYRAC	1 cut(s)	2815
HindII	GTYRAC	1 cut(s)	2815
HindIII	AAGCTT	1 cut(s)	2513
HphI	GGTGA	5 cut(s)	185, 1082, 1145, 1660, 2221
Hpy166II	GTNNAC	5 cut(s)	2281, 2702, 2777, 2815, 2842
Hpy8I	GTNNAC	5 cut(s)	2281, 2702, 2777, 2815, 2842
Hpy99I	CGWCG	4 cut(s)	90, 109, 539, 3059
HpyAV	CCTTC	5 cut(s)	820, 1045, 1613, 1929, 2790
HpyCH4III	ACNGT	8 cut(s)	492, 535, 982, 1470, 2150, 2275, 2725, 2941
HpyCH4IV	ACGT	4 cut(s)	1789, 1893, 2233, 2337
HpyF3I	CTNAG	7 cut(s)	14, 1256, 1264, 1636, 1872, 2292, 2954
HpySE526I	ACGT	4 cut(s)	1789, 1893, 2233, 2337
Hsp92I	GRCGYC	1 cut(s)	107
HspAI	GCGC	5 cut(s)	212, 257, 337, 774, 1600
Kpn2I	TCCGGA	2 cut(s)	1217, 2143
KroI	GCCGGC	1 cut(s)	1095
KroNI	GCCGGC	1 cut(s)	1097
Ksp22I	TGATCA	2 cut(s)	2728, 2898
LguI	GCTCTTC	1 cut(s)	783
LmnI	GCTCC	8 cut(s)	241, 280, 352, 626, 730, 1654, 1888, 2367
LweI	GCATC	6 cut(s)	177, 1813, 1972, 2180, 2623, 3141
MaeI	CTAG	7 cut(s)	462, 2529, 2625, 2648, 2969, 3008, 3041
MaeII	ACGT	4 cut(s)	1789, 1893, 2233, 2337
MaeIII	GTNAC	5 cut(s)	742, 1852, 1938, 2592, 3046
MbiI	CCGCTC	2 cut(s)	244, 370
MfeI	CAATTG	4 cut(s)	555, 1751, 1967, 2071
MflI	RGATCY	5 cut(s)	1177, 1251, 1333, 2198, 2949
MhlI	GDGCHC	6 cut(s)	20, 222, 272, 325, 1747, 1893
MlsI	TGGCCA	1 cut(s)	1002
MluNI	TGGCCA	1 cut(s)	1002
MlyI	GAGTC	3 cut(s)	1555, 2399, 3068
MmeI	TCCRAC	4 cut(s)	575, 1322, 1735, 3046
Mox20I	TGGCCA	1 cut(s)	1002
Mph1103I	ATGCAT	2 cut(s)	170, 1598
MroI	TCCGGA	2 cut(s)	1217, 2143
MroNI	GCCGGC	1 cut(s)	1095
MroXI	GAANNNNTTC	1 cut(s)	2890
MscI	TGGCCA	1 cut(s)	1002
MslI	CAYNNNNRTG	4 cut(s)	1673, 2207, 2736, 3137
Msp20I	TGGCCA	1 cut(s)	1002
MspA1I	CMGCKG	3 cut(s)	512, 2051, 3028
MunI	CAATTG	4 cut(s)	555, 1751, 1967, 2071
Mva1269I	GAATGC	1 cut(s)	1486
MvaI	CCWGG	5 cut(s)	239, 326, 1204, 1231, 1493
MvnI	CGCG	4 cut(s)	542, 776, 895, 2364
NaeI	GCCGGC	1 cut(s)	1097
NciI	CCSGG	7 cut(s)	273, 274, 350, 616, 990, 996, 2207
NgoMIV	GCCGGC	1 cut(s)	1095
NmeAIII	GCCGAG	1 cut(s)	3150
NmuCI	GTSAC	2 cut(s)	742, 3046
NsiI	ATGCAT	2 cut(s)	170, 1598
NspI	RCATGY	7 cut(s)	168, 604, 1596, 1600, 1678, 1918, 2125
NspV	TTCGAA	2 cut(s)	908, 1342
OliI	CACNNNNGTG	1 cut(s)	2207
PaeI	GCATGC	3 cut(s)	168, 1600, 1918
PaeR7I	CTCGAG	2 cut(s)	215, 392
PagI	TCATGA	1 cut(s)	2731
PceI	AGGCCT	1 cut(s)	12
PciI	ACATGT	2 cut(s)	600, 2121
PciSI	GCTCTTC	1 cut(s)	783
PcsI	WCGNNNNNNNCGW	1 cut(s)	3075
PctI	GAATGC	1 cut(s)	1486
PdiI	GCCGGC	1 cut(s)	1097
PdmI	GAANNNNTTC	1 cut(s)	2890
PflMI	CCANNNNNTGG	1 cut(s)	1009
Ple19I	CGATCG	1 cut(s)	72
PleI	GAGTC	3 cut(s)	1555, 2399, 3067
PpsI	GAGTC	3 cut(s)	1555, 2399, 3067
PscI	ACATGT	2 cut(s)	600, 2121
Psp124BI	GAGCTC	1 cut(s)	222
Psp6I	CCWGG	5 cut(s)	237, 324, 1202, 1229, 1491
PspGI	CCWGG	5 cut(s)	237, 324, 1202, 1229, 1491
PspOMI	GGGCCC	1 cut(s)	321
PspPI	GGNCC	9 cut(s)	321, 322, 328, 581, 986, 992, 1828, 2141, 2281
PspXI	VCTCGAGB	2 cut(s)	215, 392
PstI	CTGCAG	1 cut(s)	624
PstNI	CAGNNNCTG	3 cut(s)	345, 1549, 2051
PsuI	RGATCY	5 cut(s)	1177, 1251, 1333, 2198, 2949
PvuI	CGATCG	1 cut(s)	72
PvuII	CAGCTG	1 cut(s)	3028
RsaI	GTAC	4 cut(s)	1369, 1679, 2572, 2776
RsaNI	GTAC	4 cut(s)	1368, 1678, 2571, 2775
RseI	CAYNNNNRTG	4 cut(s)	1673, 2207, 2736, 3137
SacI	GAGCTC	1 cut(s)	222
SapI	GCTCTTC	1 cut(s)	783
Sau96I	GGNCC	9 cut(s)	321, 322, 328, 581, 986, 992, 1828, 2141, 2281
SchI	GAGTC	3 cut(s)	1555, 2399, 3068
SduI	GDGCHC	6 cut(s)	20, 222, 272, 325, 1747, 1893
SfaNI	GCATC	6 cut(s)	177, 1813, 1972, 2180, 2623, 3141
SfcI	CTRYAG	3 cut(s)	620, 1181, 1766
SfiI	GGCCNNNNNGGCC	1 cut(s)	999
Sfr274I	CTCGAG	2 cut(s)	215, 392
SfuI	TTCGAA	2 cut(s)	908, 1342
SinI	GGWCC	5 cut(s)	581, 986, 1828, 2141, 2281
SlaI	CTCGAG	2 cut(s)	215, 392
SmaI	CCCGGG	1 cut(s)	274
SmiI	ATTTAAAT	1 cut(s)	609
SmiMI	CAYNNNNRTG	4 cut(s)	1673, 2207, 2736, 3137
SmlI	CTYRAG	3 cut(s)	215, 392, 2330
SmoI	CTYRAG	3 cut(s)	215, 392, 2330
SphI	GCATGC	3 cut(s)	168, 1600, 1918
SseBI	AGGCCT	1 cut(s)	12
SspMI	CTAG	7 cut(s)	462, 2529, 2625, 2648, 2969, 3008, 3041
SstI	GAGCTC	1 cut(s)	222
StuI	AGGCCT	1 cut(s)	12
StyI	CCWWGG	4 cut(s)	2624, 2792, 2919, 3040
SwaI	ATTTAAAT	1 cut(s)	609
TaaI	ACNGT	8 cut(s)	492, 535, 982, 1470, 2150, 2275, 2725, 2941
TaiI	ACGT	4 cut(s)	1792, 1896, 2236, 2340
TaqII	GACCGA	1 cut(s)	2298
TatI	WGTACW	4 cut(s)	1367, 1677, 2570, 2774
TscAI	CASTG	9 cut(s)	209, 235, 433, 1194, 1475, 1935, 2155, 2458, 2844
TseFI	GTSAC	2 cut(s)	742, 3046
Tsp45I	GTSAC	2 cut(s)	742, 3046
TspGWI	ACGGA	3 cut(s)	1841, 2252, 3165
TspMI	CCCGGG	1 cut(s)	272
TspRI	CASTG	9 cut(s)	209, 235, 433, 1194, 1475, 1935, 2155, 2458, 2844
Van91I	CCANNNNNTGG	1 cut(s)	1009
VpaK11BI	GGWCC	5 cut(s)	581, 986, 1828, 2141, 2281
XapI	RAATTY	2 cut(s)	544, 830
XceI	RCATGY	7 cut(s)	168, 604, 1596, 1600, 1678, 1918, 2125
XcmI	CCANNNNNNNNNTGG	2 cut(s)	301, 2077
XhoI	CTCGAG	2 cut(s)	215, 392
XmaI	CCCGGG	1 cut(s)	272
XmaJI	CCTAGG	2 cut(s)	2624, 3040
XmnI	GAANNNNTTC	1 cut(s)	2890
XspI	CTAG	7 cut(s)	462, 2529, 2625, 2648, 2969, 3008, 3041
Zsp2I	ATGCAT	2 cut(s)	170, 1598

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.