Rroxscaffold_5G00348750

chaperone protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013028
Physical Location & Seq
Reverse (-)
21178281 .. 21183123
4843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348750.1

Sequence Viewer

Length: 3321 bp
ATGAGAGCAGGCCTGAGCACGATCCAGCAAACCTTAACGCCGGAGGCGGCGAGCGTATTGAACCACTCGATCGCCGAAGCGGGTCGTCGGAACCACGGCCAAACGACGCCCCTTCATGTAGCGGCAACTCTTCTATCTTCCCCCACCGGCTTTCTCCGCCAAGCATGCATCAAATCTCACCCCAATTCCTCTCACCCTCTTCAGTGCCGAGCGCTGGAGCTCTGCTTCAGTGTGGCGCTGGAGCGGTTGCCCACCGCCCAGAACATGAGCCCCGGGATGGAGCCACCAATCTCCAATGCCCTCATGGCCGCCTTAAAGCGAGCTCAGGCTCACCAGCGCAGAGGCTGCCCGGAGCAGCAGCAACAGCCGCTCCTAGCTGTCAAAGTCGAACTCGAGCAGCTCATAATCTCGATTCTCGATGATCCAAGTGTTAGTCGGGTCATGCGGGAGGCCAGTTTCTCTAGTCCGGCTGTTAAAGCCACCATTGAACAATCTCTAAACTCTTCATCCGCCGCCGCGGCAGCGGCCTCCACCGCCGCCGCGGCGAATTCTTCCTCAATTGGATTGGGGTTCCGGCCGGCGGTCCCGGCCGCCGGCCGGAACGTGTATTTGAATCCCAGGCTACAGGGGACCGCTGGGAATTCGGGACAAAACCGAGCCGAGGAAGTTAAAAAAGTCGTTGATATTTTGTCTAGGGGCAAGAAGAGGAATCCGGTATTGGTCGGCGATACGGAGCCCGAGGCAGTGACGAAGGAGCTATTCAGGCGGATTCAGAGCATGGAATTGGGGCAAGGGCACCTAAAAAATGTCGAGGTTATTCATTTGGAGAAGGAGTTTTCTTCGCAAATATTGGGGAAGATGAAGGACTTGATGTCCTTGGTTGAGACCCGGATGGCGAGTTCGAATGGGGGAGGGATGATTCTTGATTTGGGCGACTTGAAATGGCTCGTAGAGCAGCCTGTTAGCTTGGGAGCGGTTCCGCCCGGCTCCGGTGGCCAACAGGTGGTTTCGGAGGCCGGGCGGGCGGCGGTGGCGGAAATGGGGAAGGTGTTGGGAAGGTTTGCAGAGGGTGGTGCTAATGGTAGTGGCCGGCTTTGGTTGATAGGGACTGCTACTTGTGAGACGTATTTGAGGTGCCAAGTCTATCACCCTTCAATGGAAACGGACTGGGATCTACAGGCAGTGCCGATTGCTGCCAGGACGCCGCATTCGGGATTGTTTTCTAGTTTATGGAAGCTTGAGTACTGCTCTATTAAATTCTTCTGTGCAAAGCTATTATGGAAGAGGGAGTTCCTTAATTTCAAAATCAAAGAGTATCTGATGGAATCTGAACTGATATTTGTTGCCTGGCAGGGGTCTAGTTCTAGCTTCACTATAAACTTGATGGGGACGACGAACGGGATCCTTAGTAGCTCAGTTGAATCTTTATCTCCGTTGAAAGGCTTTTCAACTGCTCAACCAAGGCTTGTGCCTGAGAACTTGGATGCTGCACGGAGAACAAGCTGTTGCCCGCAATGTACAGGAAGTTGTGAGCAAGAAGTAGCAAAATTGGTTGCGAAGGAGTCTGAGAAATCATCCTCTGAGTCAAAATCGGAAACTGCCCAACCAGCAATACCACAGTGGTTGCAGAATGCTAAAGCCCAGGACAGTAATGTCAAAACATCAGATCAGTTGCAGACTAAGAATCAAGATCAGACTTTGAACCAGAAGACTCAAGAATTACGAAAGGAATGGAAAGATACGTGTATGCGCCTTCATCCTAATTTTCATCAGCAGAGTTTCGGCTCAGAGAGAATTGCTCCTGCACCTCTCTCAATCACCAGCATGTACAATATGAACTTGCTTGGTCGCCAACCTTTCCAACCGAAATTGCAACCAAACAAAAGCTTTGGGGCTCTGCAATTGAACACAAATCTAAAGACCAGCCAACCATCTGAACGTGCAGCAGTTTCACATCCACGAAGCCCCGTAAGGACAGAGCTGGTTCTTGGGCAAAAAGAAGTCACTGAAACCATCACCCCTGAGCAAATGCATAAGGAGCGCGTCAAAGACTCTATGGGCTGCATGCCTTCCGAACCACAGAACAAGATACTTGAAAGGCAAACAGATGAAAAGCAATTGTGCCAATTAGATGCTGATTCATTCAAAAAGCTGTACAAGGGTTTGATGGAGGTCTGGTGGCAGCCGGAAGCAGCAAATGCTGTTGCTGCTACGATAACCAATTGTAAGTTGGGTAATGTAAAGAGGCGTGGTGTCGGAGCAAGAGGAGACATGTGGTTGTTTTTCATGGGTCCTGACAGTGTTGGCAAGAAGAAGATGACATCAGCTCTTTCAGAAATGGTATCTGGGTCCACACCGGTGATGATCTCCCTTAACAATAAACGCGGTAGTTGGGAATCAGACATGAGTTTTCGTGGAAAAATGGTGGTGGACCGAATAGCAGAGGCAGTTAGGAGGAACCCCTTTTCTGTAATCATGCTTGAGGACATTAATGAAGCAGATACGATAGTTCGCAGTAGCATAAAACGGGCAATGGAGAGAGGTCGTCTTGGCGACTCTTATGGCCGTGAAATCAGTCTTGGAAATGTTATCTTTATCCTCACTGCAAATTGGTTGCCAGAAAATTTGAAACACTTATCAAGCGGCAATTCTCTTGAAGAAAAGCTTGCTTGTATAGCTAGGAGTGGTTGGCAGTTAAAGCTATCCGTTTGTGGAAGAAGTGCAAAACGGCGAGCAACTTGGTTACAGAGTGTTGAAGACAGAGCCACTAAGCCTAGGAAAGATGCGGGTTCTGGACTAGGATTTGATCTGAATGAGGCTGCTGATGTTGAGGATGACAGAACAGATGGCTCACTTAATTCAAGTGATCTAACAGTTGATCATGAAGATGAGAACCGCCTCAGTAACAGGTCATTGCTCACAAGTACAAGTACACCTTCATCAGTACTGAGGGAATTACTTGATTCTGTTGACGATGCCATTGTGTTCAAGCCAGTGGATTTCAATCCCATTCGGAATAACATCACAAACTCAATCACAAGAAGTTTTTCAATGATCATTGGCGATGGGGTTTCCTTTGAATTGCAAGAGGATACTGTTGAAAAGATCCTTGGTGGGATATGGCTAGGCAAGACTGGCTTAGAAGAATGGACTGAAAAGGTGCTGGTTCCGAGCCTCCAACAGTTGAAGTCCCGCCCAGGTGTGACACTTGACGAGTCAATGGTGGTTAGACTCGAAGATGATGGAGATTCGGGTTGCCGGAGACATGGAGATTGGCTGCCGAGTAGCATCAATGTGGTGGCCGACGGATTAAGACAATGA

Protein Analysis

1106

Amino Acids

121.28

Weight (kDa)

8.36

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 113 1.2e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 219 - 400 2.2e-29 SMAX1 nucleotide binding domain
AAA_2 PF07724 761 - 877 2.4e-09 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 1000 - 1083 3.9e-25 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57710
fragaria_vesca FvH4_4g09820
malus_domestica MD13G1261900.v1.1 MD16G1264200.v1.1
prunus_persica Prupe.1G095200_v2.0.a1 Prupe.1G095200_v2.0.a1
pyrus_communis pycom13g24720 pycom16g23290
rosa_chinensis RchiOBHm_Chr4g0404601
rosa_laevigata RLG00000008920
rosa_multiflora Rmu_sc0002699.1_g000006
rosa_roxburghii Rroxscaffold_5G00348750
rosa_rugosa Rorug04G0047700
rosa_samantha Rh4AG123800 Rh4BG117200 Rh4CG131900 Rh4DG116700
rosa_wichuraiana Rw4G010030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1652
AccB1I GGYRCC 2 cut(s) 795, 1134
AccB7I CCANNNNNTGG 1 cut(s) 1003
AccBSI CCGCTC 3 cut(s) 244, 370, 974
AccII CGCG 4 cut(s) 518, 542, 2043, 2385
AclWI GGATC 6 cut(s) 16, 416, 1179, 1396, 1409, 3100
AcoI YGGCCR 9 cut(s) 97, 306, 575, 588, 595, 994, 1087, 2563, 3300
AcsI RAATTY 4 cut(s) 547, 640, 1256, 2623
AcuI CTGAAG 2 cut(s) 185, 211
AcyI GRCGYC 2 cut(s) 107, 1202
AfaI GTAC 7 cut(s) 1244, 1519, 1829, 2156, 2926, 2932, 2946
AfeI AGCGCT 1 cut(s) 213
AflIII ACRYGT 3 cut(s) 603, 1742, 2271
AgeI ACCGGT 1 cut(s) 2356
AhdI GACNNNNNGTC 1 cut(s) 871
AjnI CCWGG 5 cut(s) 617, 1196, 1346, 1641, 3196
AjuI GAANNNNNNNTTGG 6 cut(s) 701, 733, 2562, 2594, 3093, 3125
AleI CACNNNNGTG 1 cut(s) 2357
AloI GAACNNNNNNTCC 2 cut(s) 883, 915
Alw21I GWGCWC 3 cut(s) 20, 222, 325
Alw26I GTCTC 4 cut(s) 878, 1115, 2262, 3256
AlwI GGATC 6 cut(s) 16, 416, 1179, 1396, 1409, 3100
AlwNI CAGNNNCTG 1 cut(s) 345
Ama87I CYCGRG 3 cut(s) 272, 392, 737
Aor51HI AGCGCT 1 cut(s) 213
ApoI RAATTY 4 cut(s) 547, 640, 1256, 2623
ArsI GACNNNNNNTTYG 2 cut(s) 1192, 1224
AseI ATTAAT 1 cut(s) 2490
AsiGI ACCGGT 1 cut(s) 2356
Asp700I GAANNNNTTC 1 cut(s) 3046
AspA2I CCTAGG 1 cut(s) 2774
AspLEI GCGC 5 cut(s) 214, 238, 339, 1752, 2043
AspS9I GGNCC 5 cut(s) 583, 630, 2291, 2349, 2431
AsuC2I CCSGG 7 cut(s) 273, 274, 350, 587, 889, 984, 1018
AsuHPI GGTGA 7 cut(s) 170, 185, 323, 1139, 1810, 2008, 2371
AsuII TTCGAA 1 cut(s) 902
AvaI CYCGRG 3 cut(s) 272, 392, 737
AvaII GGWCC 5 cut(s) 583, 630, 2291, 2349, 2431
AvrII CCTAGG 1 cut(s) 2774
BaeGI GKGCMC 1 cut(s) 798
BaeI ACNNNNGTAYC 2 cut(s) 2493, 2526
BalI TGGCCA 1 cut(s) 996
BamHI GGATCC 1 cut(s) 1401
BanI GGYRCC 2 cut(s) 795, 1134
BanII GRGCYC 5 cut(s) 222, 272, 325, 738, 1897
BarI GAAGNNNNNNTAC 2 cut(s) 1226, 1258
BbsI GAAGAC 2 cut(s) 1715, 2763
Bbv12I GWGCWC 3 cut(s) 20, 222, 325
BceAI ACGGC 3 cut(s) 112, 2550, 2744
BciT130I CCWGG 5 cut(s) 619, 1198, 1348, 1643, 3198
BciVI GTATCC 1 cut(s) 3085
BclI TGATCA 2 cut(s) 2878, 3054
BcnI CCSGG 7 cut(s) 273, 274, 350, 587, 889, 984, 1018
BcoDI GTCTC 4 cut(s) 878, 1115, 2262, 3256
BfmI CTRYAG 2 cut(s) 623, 1175
BfoI RGCGCY 2 cut(s) 215, 239
BfuI GTATCC 1 cut(s) 3085
BglI GCCNNNNNGGC 4 cut(s) 305, 518, 542, 1022
BlnI CCTAGG 1 cut(s) 2774
BmcAI AGTACT 2 cut(s) 1244, 2946
Bme18I GGWCC 5 cut(s) 583, 630, 2291, 2349, 2431
BmeRI GACNNNNNGTC 1 cut(s) 871
BmeT110I CYCGRG 3 cut(s) 272, 392, 737
BmgT120I GGNCC 5 cut(s) 583, 630, 2291, 2349, 2431
BmrI ACTGGG 1 cut(s) 1177
BmsI GCATC 6 cut(s) 177, 1474, 2122, 2773, 2965, 3297
BmuI ACTGGG 1 cut(s) 1177
BpiI GAAGAC 2 cut(s) 1715, 2763
BplI GAGNNNNNCTC 4 cut(s) 1232, 1264, 1783, 1815
BpmI CTGGAG 2 cut(s) 236, 260
Bpu10I CCTNAGC 3 cut(s) 14, 324, 2022
Bpu14I TTCGAA 1 cut(s) 902
BpuEI CTTGAG 3 cut(s) 1259, 1698, 2501
BpuMI CCSGG 7 cut(s) 273, 274, 350, 587, 889, 984, 1018
BsaAI YACGTR 1 cut(s) 1743
BsaBI GATNNNNATC 1 cut(s) 3003
BsaHI GRCGYC 2 cut(s) 107, 1202
BsaI GGTCTC 1 cut(s) 878
BsaWI WCCGGW 3 cut(s) 712, 989, 2356
BsaXI ACNNNNNCTCC 6 cut(s) 354, 384, 818, 848, 2162, 2192
Bse118I RCCGGY 5 cut(s) 146, 577, 593, 1089, 2356
Bse1I ACTGG 4 cut(s) 453, 1172, 2993, 3139
Bse3DI GCAATG 3 cut(s) 1520, 2538, 2912
Bse8I GATNNNNATC 1 cut(s) 3003
BseBI CCWGG 5 cut(s) 619, 1198, 1348, 1643, 3198
BseGI GGATG 9 cut(s) 282, 506, 897, 921, 1489, 1574, 1756, 1954, 2839
BseJI GATNNNNATC 1 cut(s) 3003
BseMI GCAATG 3 cut(s) 1520, 2538, 2912
BseNI ACTGG 4 cut(s) 453, 1172, 2993, 3139
BseRI GAGGAG 1 cut(s) 2280
BseSI GKGCMC 1 cut(s) 798
BseX3I CGGCCG 3 cut(s) 575, 588, 595
BseYI CCCAGC 1 cut(s) 635
BsgI GTGCAG 3 cut(s) 1473, 1788, 1962
Bsh1236I CGCG 4 cut(s) 518, 542, 2043, 2385
Bsh1285I CGRYCG 4 cut(s) 72, 578, 591, 598
BshNI GGYRCC 2 cut(s) 795, 1134
BshTI ACCGGT 1 cut(s) 2356
BsiEI CGRYCG 4 cut(s) 72, 578, 591, 598
BsiHKAI GWGCWC 3 cut(s) 20, 222, 325
BsiHKCI CYCGRG 3 cut(s) 272, 392, 737
BslFI GGGAC 6 cut(s) 569, 643, 660, 1120, 1402, 3175
BsmAI GTCTC 4 cut(s) 878, 1115, 2262, 3256
BsmBI CGTCTC 1 cut(s) 1115
BsmFI GGGAC 6 cut(s) 569, 643, 660, 1120, 1402, 3175
BsmI GAATGC 2 cut(s) 1207, 1636
Bso31I GGTCTC 1 cut(s) 878
BsoBI CYCGRG 3 cut(s) 272, 392, 737
Bsp119I TTCGAA 1 cut(s) 902
Bsp1286I GDGCHC 7 cut(s) 20, 222, 272, 325, 738, 798, 1897
Bsp1407I TGTACA 3 cut(s) 1517, 1827, 2154
BspFNI CGCG 4 cut(s) 518, 542, 2043, 2385
BspHI TCATGA 1 cut(s) 2881
BspPI GGATC 6 cut(s) 16, 416, 1179, 1396, 1409, 3100
BspT104I TTCGAA 1 cut(s) 902
BspT107I GGYRCC 2 cut(s) 795, 1134
BspTNI GGTCTC 1 cut(s) 878
BsrBI CCGCTC 3 cut(s) 244, 370, 974
BsrDI GCAATG 3 cut(s) 1520, 2538, 2912
BsrFI RCCGGY 5 cut(s) 146, 577, 593, 1089, 2356
BsrGI TGTACA 3 cut(s) 1517, 1827, 2154
BsrI ACTGG 4 cut(s) 453, 1172, 2993, 3139
BssAI RCCGGY 5 cut(s) 146, 577, 593, 1089, 2356
BssNI GRCGYC 2 cut(s) 107, 1202
BssT1I CCWWGG 4 cut(s) 876, 1460, 2774, 3109
Bst2UI CCWGG 5 cut(s) 619, 1198, 1348, 1643, 3198
Bst4CI ACNGT 6 cut(s) 1620, 1649, 2300, 2875, 3097, 3183
Bst6I CTCTTC 5 cut(s) 135, 204, 508, 698, 1277
BstACI GRCGYC 2 cut(s) 107, 1202
BstAPI GCANNNNNTGC 2 cut(s) 345, 2198
BstAUI TGTACA 3 cut(s) 1517, 1827, 2154
BstBAI YACGTR 1 cut(s) 1743
BstBI TTCGAA 1 cut(s) 902
BstDSI CCRYGG 3 cut(s) 94, 516, 540
BstF5I GGATG 9 cut(s) 282, 506, 897, 921, 1489, 1574, 1756, 1954, 2839
BstFNI CGCG 4 cut(s) 518, 542, 2043, 2385
BstH2I RGCGCY 2 cut(s) 215, 239
BstHHI GCGC 5 cut(s) 214, 238, 339, 1752, 2043
BstMAI GTCTC 4 cut(s) 878, 1115, 2262, 3256
BstMCI CGRYCG 4 cut(s) 72, 578, 591, 598
BstNI CCWGG 5 cut(s) 619, 1198, 1348, 1643, 3198
BstNSI RCATGY 4 cut(s) 168, 1828, 2068, 2275
BstSFI CTRYAG 2 cut(s) 623, 1175
BstSLI GKGCMC 1 cut(s) 798
BstUI CGCG 4 cut(s) 518, 542, 2043, 2385
BstV2I GAAGAC 2 cut(s) 1715, 2763
BstX2I RGATCY 3 cut(s) 1171, 1401, 3105
BstYI RGATCY 3 cut(s) 1171, 1401, 3105
BstZI CGGCCG 3 cut(s) 575, 588, 595
BsuI GTATCC 1 cut(s) 3085
BtgI CCRYGG 3 cut(s) 94, 516, 540
BtgZI GCGATG 1 cut(s) 3078
BtsCI GGATG 9 cut(s) 282, 506, 897, 921, 1489, 1574, 1756, 1954, 2839
BtsI GCAGTG 3 cut(s) 750, 1188, 2601
BtsIMutI CAGTG 9 cut(s) 209, 235, 750, 1188, 1625, 2004, 2305, 2601, 3000
CaiI CAGNNNCTG 1 cut(s) 345
CciI TCATGA 1 cut(s) 2881
CfoI GCGC 5 cut(s) 214, 238, 339, 1752, 2043
Cfr10I RCCGGY 5 cut(s) 146, 577, 593, 1089, 2356
Cfr13I GGNCC 5 cut(s) 583, 630, 2291, 2349, 2431
Cfr42I CCGCGG 2 cut(s) 519, 543
Cfr9I CCCGGG 1 cut(s) 272
CseI GACGC 3 cut(s) 115, 1210, 2032
Csp6I GTAC 7 cut(s) 1243, 1518, 1828, 2155, 2925, 2931, 2945
CspAI ACCGGT 1 cut(s) 2356
CspCI CAANNNNNGTGG 2 cut(s) 1605, 1640
CviQI GTAC 7 cut(s) 1243, 1518, 1828, 2155, 2925, 2931, 2945
DrdI GACNNNNNNGTC 1 cut(s) 1652
DriI GACNNNNNGTC 1 cut(s) 871
DseDI GACNNNNNNGTC 1 cut(s) 1652
EaeI YGGCCR 9 cut(s) 97, 306, 575, 588, 595, 994, 1087, 2563, 3300
EagI CGGCCG 3 cut(s) 575, 588, 595
Eam1104I CTCTTC 5 cut(s) 135, 204, 508, 698, 1277
Eam1105I GACNNNNNGTC 1 cut(s) 871
EarI CTCTTC 5 cut(s) 135, 204, 508, 698, 1277
EciI GGCGGA 5 cut(s) 146, 499, 781, 969, 1049
Ecl136II GAGCTC 2 cut(s) 220, 323
EclXI CGGCCG 3 cut(s) 575, 588, 595
Eco130I CCWWGG 4 cut(s) 876, 1460, 2774, 3109
Eco147I AGGCCT 1 cut(s) 12
Eco24I GRGCYC 5 cut(s) 222, 272, 325, 738, 1897
Eco31I GGTCTC 1 cut(s) 878
Eco47I GGWCC 5 cut(s) 583, 630, 2291, 2349, 2431
Eco47III AGCGCT 1 cut(s) 213
Eco52I CGGCCG 3 cut(s) 575, 588, 595
Eco53kI GAGCTC 2 cut(s) 220, 323
Eco57I CTGAAG 2 cut(s) 185, 211
Eco88I CYCGRG 3 cut(s) 272, 392, 737
EcoICRI GAGCTC 2 cut(s) 220, 323
EcoO109I RGGNCCY 1 cut(s) 2291
EcoRI GAATTC 2 cut(s) 547, 640
EcoRII CCWGG 5 cut(s) 617, 1196, 1346, 1641, 3196
EcoT14I CCWWGG 4 cut(s) 876, 1460, 2774, 3109
EcoT22I ATGCAT 2 cut(s) 170, 2034
EcoT38I GRGCYC 5 cut(s) 222, 272, 325, 738, 1897
ErhI CCWWGG 4 cut(s) 876, 1460, 2774, 3109
Esp3I CGTCTC 1 cut(s) 1115
FalI AAGNNNNNCTT 6 cut(s) 2649, 2681, 2920, 2952, 3122, 3154
FaqI GGGAC 6 cut(s) 569, 643, 660, 1120, 1402, 3175
FauI CCCGC 6 cut(s) 73, 438, 1014, 1518, 2779, 3200
FbaI TGATCA 2 cut(s) 2878, 3054
FokI GGATG 9 cut(s) 289, 493, 904, 928, 1496, 1561, 1743, 1941, 2846
FriOI GRGCYC 5 cut(s) 222, 272, 325, 738, 1897
GlaI GCGC 5 cut(s) 213, 237, 338, 1751, 2042
GsaI CCCAGC 1 cut(s) 639
GsuI CTGGAG 2 cut(s) 236, 260
HaeII RGCGCY 2 cut(s) 215, 239
HgaI GACGC 3 cut(s) 115, 1210, 2032
HhaI GCGC 5 cut(s) 214, 238, 339, 1752, 2043
Hin1I GRCGYC 2 cut(s) 107, 1202
Hin6I GCGC 5 cut(s) 212, 236, 337, 1750, 2041
HinP1I GCGC 5 cut(s) 212, 236, 337, 1750, 2041
HincII GTYRAC 1 cut(s) 2971
HindII GTYRAC 1 cut(s) 2971
HindIII AAGCTT 3 cut(s) 1235, 1885, 2663
HphI GGTGA 7 cut(s) 170, 185, 323, 1139, 1810, 2008, 2371
Hpy166II GTNNAC 4 cut(s) 2352, 2431, 2933, 2971
Hpy8I GTNNAC 4 cut(s) 2352, 2431, 2933, 2971
Hpy99I CGWCG 4 cut(s) 90, 109, 1396, 3308
HpyCH4III ACNGT 6 cut(s) 1620, 1649, 2300, 2875, 3097, 3183
HpyCH4IV ACGT 4 cut(s) 603, 1124, 1742, 1939
HpySE526I ACGT 4 cut(s) 603, 1124, 1742, 1939
Hsp92I GRCGYC 2 cut(s) 107, 1202
HspAI GCGC 5 cut(s) 212, 236, 337, 1750, 2041
KroI GCCGGC 3 cut(s) 577, 593, 1089
KroNI GCCGGC 3 cut(s) 579, 595, 1091
Ksp22I TGATCA 2 cut(s) 2878, 3054
KspI CCGCGG 2 cut(s) 519, 543
LweI GCATC 6 cut(s) 177, 1474, 2122, 2773, 2965, 3297
MaeII ACGT 4 cut(s) 603, 1124, 1742, 1939
MaeIII GTNAC 5 cut(s) 745, 2002, 2742, 2903, 3202
MbiI CCGCTC 3 cut(s) 244, 370, 974
MfeI CAATTG 4 cut(s) 558, 1901, 2117, 2221
MflI RGATCY 3 cut(s) 1171, 1401, 3105
MhlI GDGCHC 7 cut(s) 20, 222, 272, 325, 738, 798, 1897
MlsI TGGCCA 1 cut(s) 996
MluNI TGGCCA 1 cut(s) 996
MlyI GAGTC 7 cut(s) 1571, 1592, 1705, 2045, 2549, 3224, 3225
MmeI TCCRAC 4 cut(s) 68, 1885, 2236, 3202
Mox20I TGGCCA 1 cut(s) 996
Mph1103I ATGCAT 2 cut(s) 170, 2034
MroNI GCCGGC 3 cut(s) 577, 593, 1089
MroXI GAANNNNTTC 1 cut(s) 3046
MscI TGGCCA 1 cut(s) 996
MslI CAYNNNNRTG 4 cut(s) 1823, 2357, 2886, 3293
Msp20I TGGCCA 1 cut(s) 996
MspA1I CMGCKG 4 cut(s) 518, 524, 542, 635
MunI CAATTG 4 cut(s) 558, 1901, 2117, 2221
Mva1269I GAATGC 2 cut(s) 1207, 1636
MvaI CCWGG 5 cut(s) 619, 1198, 1348, 1643, 3198
MvnI CGCG 4 cut(s) 518, 542, 2043, 2385
NaeI GCCGGC 3 cut(s) 579, 595, 1091
NciI CCSGG 7 cut(s) 273, 274, 350, 587, 889, 984, 1018
NgoMIV GCCGGC 3 cut(s) 577, 593, 1089
NmeAIII GCCGAG 3 cut(s) 233, 685, 3306
NmuCI GTSAC 3 cut(s) 745, 2002, 3202
NsiI ATGCAT 2 cut(s) 170, 2034
NspI RCATGY 4 cut(s) 168, 1828, 2068, 2275
NspV TTCGAA 1 cut(s) 902
OliI CACNNNNGTG 1 cut(s) 2357
PaeI GCATGC 2 cut(s) 168, 2068
PaeR7I CTCGAG 1 cut(s) 392
PagI TCATGA 1 cut(s) 2881
PceI AGGCCT 1 cut(s) 12
PciI ACATGT 1 cut(s) 2271
PctI GAATGC 2 cut(s) 1207, 1636
PdiI GCCGGC 3 cut(s) 579, 595, 1091
PdmI GAANNNNTTC 1 cut(s) 3046
PflMI CCANNNNNTGG 1 cut(s) 1003
PinAI ACCGGT 1 cut(s) 2356
Ple19I CGATCG 1 cut(s) 72
PleI GAGTC 7 cut(s) 1570, 1591, 1705, 2045, 2549, 3223, 3225
PpsI GAGTC 7 cut(s) 1570, 1591, 1705, 2045, 2549, 3223, 3225
Ppu21I YACGTR 1 cut(s) 1743
PpuMI RGGWCCY 1 cut(s) 2291
PscI ACATGT 1 cut(s) 2271
PshBI ATTAAT 1 cut(s) 2490
Psp124BI GAGCTC 2 cut(s) 222, 325
Psp5II RGGWCCY 1 cut(s) 2291
Psp6I CCWGG 5 cut(s) 617, 1196, 1346, 1641, 3196
PspFI CCCAGC 1 cut(s) 635
PspGI CCWGG 5 cut(s) 617, 1196, 1346, 1641, 3196
PspPI GGNCC 5 cut(s) 583, 630, 2291, 2349, 2431
PspPPI RGGWCCY 1 cut(s) 2291
PspXI VCTCGAGB 1 cut(s) 392
PstNI CAGNNNCTG 1 cut(s) 345
PsuI RGATCY 3 cut(s) 1171, 1401, 3105
PvuI CGATCG 1 cut(s) 72
RsaI GTAC 7 cut(s) 1244, 1519, 1829, 2156, 2926, 2932, 2946
RsaNI GTAC 7 cut(s) 1243, 1518, 1828, 2155, 2925, 2931, 2945
RseI CAYNNNNRTG 4 cut(s) 1823, 2357, 2886, 3293
SacI GAGCTC 2 cut(s) 222, 325
SacII CCGCGG 2 cut(s) 519, 543
Sau96I GGNCC 5 cut(s) 583, 630, 2291, 2349, 2431
ScaI AGTACT 2 cut(s) 1244, 2946
SchI GAGTC 7 cut(s) 1571, 1592, 1705, 2045, 2549, 3224, 3225
SduI GDGCHC 7 cut(s) 20, 222, 272, 325, 738, 798, 1897
SfaNI GCATC 6 cut(s) 177, 1474, 2122, 2773, 2965, 3297
SfcI CTRYAG 2 cut(s) 623, 1175
Sfr274I CTCGAG 1 cut(s) 392
Sfr303I CCGCGG 2 cut(s) 519, 543
SfuI TTCGAA 1 cut(s) 902
SgrAI CRCCGGYG 1 cut(s) 2356
SgrBI CCGCGG 2 cut(s) 519, 543
SinI GGWCC 5 cut(s) 583, 630, 2291, 2349, 2431
SlaI CTCGAG 1 cut(s) 392
SmaI CCCGGG 1 cut(s) 274
SmiMI CAYNNNNRTG 4 cut(s) 1823, 2357, 2886, 3293
SmlI CTYRAG 4 cut(s) 392, 1238, 1713, 2480
SmoI CTYRAG 4 cut(s) 392, 1238, 1713, 2480
SphI GCATGC 2 cut(s) 168, 2068
SseBI AGGCCT 1 cut(s) 12
SspI AATATT 1 cut(s) 849
SstI GAGCTC 2 cut(s) 222, 325
StuI AGGCCT 1 cut(s) 12
StyI CCWWGG 4 cut(s) 876, 1460, 2774, 3109
TaaI ACNGT 6 cut(s) 1620, 1649, 2300, 2875, 3097, 3183
TaiI ACGT 4 cut(s) 606, 1127, 1745, 1942
TaqI TCGA 8 cut(s) 68, 387, 393, 410, 417, 810, 902, 3234
TaqII GACCGA 1 cut(s) 2448
TatI WGTACW 7 cut(s) 1242, 1517, 1827, 2154, 2924, 2930, 2944
TscAI CASTG 9 cut(s) 209, 235, 750, 1188, 1625, 2011, 2305, 2608, 3000
TseFI GTSAC 3 cut(s) 745, 2002, 3202
Tsp45I GTSAC 3 cut(s) 745, 2002, 3202
TspGWI ACGGA 6 cut(s) 746, 1178, 1422, 1507, 2695, 3321
TspMI CCCGGG 1 cut(s) 272
TspRI CASTG 9 cut(s) 209, 235, 750, 1188, 1625, 2011, 2305, 2608, 3000
Van91I CCANNNNNTGG 1 cut(s) 1003
VpaK11BI GGWCC 5 cut(s) 583, 630, 2291, 2349, 2431
VspI ATTAAT 1 cut(s) 2490
XapI RAATTY 4 cut(s) 547, 640, 1256, 2623
XceI RCATGY 4 cut(s) 168, 1828, 2068, 2275
XcmI CCANNNNNNNNNTGG 2 cut(s) 301, 2227
XhoI CTCGAG 1 cut(s) 392
XmaI CCCGGG 1 cut(s) 272
XmaJI CCTAGG 1 cut(s) 2774
XmnI GAANNNNTTC 1 cut(s) 3046
ZrmI AGTACT 2 cut(s) 1244, 2946
Zsp2I ATGCAT 2 cut(s) 170, 2034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.