Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0013028

Physical Location & Seq

Genomic Coordinates Reverse (-)

22895616 .. 22899819

Gene Structure 4204 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G010030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2553 bp

ATGTATTTGAATCCCAGGCTGCAGGGGACAGCTGGGAATTCGGGACAAAACCGAGCCGAGGAAGTTAAAAAAGTCGTTGATATTTTGTCTAGGAGCAAGAAGAGGAATCCGGTATTGGTCGGCGATACGGAGCCCGAGGCTGTGACGAAAGAGCTATTCAGGCGGATTCAGAGCATGGAATTGGGGCAAGGGCACCTCAAAAATGTCGAGGTCATTCATTTGGAGAAGGAGTTTTCTTCGCAAATATTGGGGAAGATGAAGGACTTGATGTCCTTGGTTGAGACCCGGATGGCGGGTTCGAATGGAGGGGGGATGATTCTTGATTTGGGCGACTTGAAATGGCTCGTGGAGCAGCCTGTTAGCTTGGGAGCTGTTCCGCCCGGCTCCGGTGGCCAACAGGTGGTTTCGGAGGCCGGGCGGGCGGCGGTGGCGGAAATGGGGAAGGTGTTGGGAAGGTTTGGAGAGGGTGGTGTTAATGGTAGTGGCCGGCTTTGGTTGATAGGGACTGCTACTTGTGAGACGTATTTGAGGTGCCAAGTCTATCACCCTTCAATGGAAACGGACTGGGATCTACAGGCAGTGCCGATTGCTGCCAGGACGCCGCATTCGGGATTGTTTCCTAGGATGGGGATGACGAACGGGATCCTTAGTAGCTCAGTTGAATCTTTGTCTCCGTTGAAAGGCTTTTCAACTGCTCAACCAAGGCTTGTGCCTGAGAACTTAGATGCTGCACGGAGAACAAGCTGTTGCTCACAATGTACAGAAAGTTGTGAGCAAGAAGTAGCAAAATTGGTTGCGAAGGAGTCTGAGAAATCATCCTCTGAGTCAAAATCGGAAGCAGCCCAACCAGCACTACCACAGTGGTTGCAGAATGCTAAAGACCAGGACAGTAATGTCAAAACATCAGATCAATTGCAGACTAAGAATCAAGATCAGACTTTGAACCAGAAGACTGAAGAATTACGAAAGGAATGGAAAGATACGTGTAAGCGCCTTCATCCTAATTTTCATCAGCATAGTTTCAGCTCAGAGAGAATTGCTCCCACACCTCTCTCAATCACCAGCATGTACAATATGAACTTGCTTGGTCGCCAACCTTTCCAACCGAAATTGCAACCAAACAAAAGCTTTGGGGCTCTGCAATTGAACGCAAATCTAAAGACCAGCCAACCATCTGAACGTGCAGCAGTTTCACATCCACGAAGCCCTGTAAGGACGGACCTGGTTCTTGGGCAAAAAGAAGTCACTGAAACCATCACCCCTGAGCAAATGCATAAGGAGCGCGTCAAAGACTTTATGGGCTGCATGCCTTCCGAACCACAGAACAAGATACTTGAAAGGCAAACAGATGAAAAGCAATTGTGCCAATTAGATGCTGATTCATTCAAAAAGCTGTACAAGGGTTTGATTGAGGTCTGGTGGCAGCCGGAAGCAGCAAACGCTGTTGCTGCTACGATAACCAATTGTAAGTTGGGTAATGGAAAGAGGCGTGGTGCCGGAGCAAGGGGAGACATGTGGTTGTTGTTCATGGGTCCTGACACTGTTGGCAAGAAGAAGATGACATCAGCTCTTTCAGAAACGGTATCTGGGTCCACCCCGGTGATGATCTCCCTTAACAATAAACGCGGTAGTTGGGAATCAGACATGAGTTTTCGTGGAAAAACGGTGGTGGACCGAATAGCAGAGGCAGTTAGGAGGAACCCCTTTTCTGTAATCATGCTTGAGGACATTAATGAAGCAGATACGATAGTTCGCGGTAGCATAAAACGGGCAATGGAGAGAGGTCGTCTTGGCGATTCTTATGGCCGTGAAATCAGTCTTGGAAATGTCATCTTTATCCTCACTGCAAATTGGTTGCCAGAAAATTTGAAACACTTATCAAGCGGCAATTCTCTTGAAGAAAAGCTTGCTTGTATAGCTAGGAGTGGTTGGCAGTTAAAGCTATCCGTTTGTGGAAGAAGTGCAAAACGGCGAGCAACTTGGTTACAGAGTGTTGAAGACAGAGCCACAAAGCCTAGGAAAGATGCGGGTTCTGGACTAGGATTCGATCTGAATGAGACTGCTGATGTTGAGGATGACAGAACAGATGGCTCACTTAATTCAAGTGATCTAACAGTTGATCATGAAGATGACCGCCTCAATAACAGGTCATTGCTCACAAGTACACCTTCATCAGTACTGAGGGAATTGCTTGATTCTGTTGACGATGCCATTGTGTTCAAGCCAGTGGATTTCAATCCCATTCGGAATAACATCACAAACTCAATCACAAGAAAATTTTCAATGATCATTGGCGATGGAGTTTCCTTTGAATTGCAAGAGGATACTGTTGAAAAGATCCTTAGTGGGATATGGCTAGGCAAGACTGGCTTAGAAGAATGGACTGAAAAGGTGCTGGTTCCGAGCCTCCAACAGTTGAAGTCCCGCCTAGGTGTGACACTTGACGAGTCAATGGTGGTTAGACTCGAAGATGATGGAGATTCGGGTTGCCGGAGACATGGAGATTGGCTGCCCAGTAGCATCAATATGGTGGCCGACGGATTAAGACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007275 GO:0007623 GO:0008150 GO:0009628 GO:0009719 GO:0009725 GO:0009791 GO:0009845 GO:0010033 GO:0014070 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0048511 GO:0048856 GO:0050896 GO:0080167 GO:0090351 GO:1901700 GO:1902347

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_2 Clp_N

Protein Analysis

850

Amino Acids

93.9

Weight (kDa)

6.9

Isoelectric Point (pI)

49.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NBD_SMAX1	PF23569	18 - 199	2e-29	SMAX1 nucleotide binding domain
AAA_2	PF07724	508 - 624	3.8e-09	AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1	PF26587	744 - 827	3.7e-26	SMAX1-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57710
fragaria_vesca	FvH4_4g09820
malus_domestica	MD13G1261900.v1.1 MD16G1264200.v1.1
prunus_persica	Prupe.1G095200_v2.0.a1 Prupe.1G095200_v2.0.a1
pyrus_communis	pycom13g24720 pycom16g23290
rosa_chinensis	RchiOBHm_Chr4g0404601
rosa_laevigata	RLG00000008920
rosa_multiflora	Rmu_sc0002699.1_g000006
rosa_roxburghii	Rroxscaffold_5G00348750
rosa_rugosa	Rorug04G0047700
rosa_samantha	Rh4AG123800 Rh4BG117200 Rh4CG131900 Rh4DG116700
rosa_wichuraiana	Rw4G010030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	893
AccB1I	GGYRCC	3 cut(s)	192, 531, 1493
AccB7I	CCANNNNNTGG	1 cut(s)	400
AccII	CGCG	3 cut(s)	1284, 1626, 1755
AclWI	GGATC	4 cut(s)	576, 637, 650, 2332
AcoI	YGGCCR	4 cut(s)	391, 484, 1804, 2532
AcsI	RAATTY	3 cut(s)	37, 1864, 2276
AcuI	CTGAAG	1 cut(s)	975
AcyI	GRCGYC	1 cut(s)	599
AfaI	GTAC	5 cut(s)	760, 1070, 1397, 2164, 2178
AfiI	CCNNNNNNNGG	9 cut(s)	58, 292, 386, 400, 553, 608, 626, 680, 1503
AflIII	ACRYGT	2 cut(s)	983, 1512
AhdI	GACNNNNNGTC	1 cut(s)	268
AjnI	CCWGG	4 cut(s)	14, 593, 882, 1221
AjuI	GAANNNNNNNTTGG	4 cut(s)	98, 130, 1803, 1835
AleI	CACNNNNGTG	1 cut(s)	1598
AloI	GAACNNNNNNTCC	2 cut(s)	280, 312
Alw26I	GTCTC	6 cut(s)	275, 512, 675, 1503, 2051, 2488
AlwI	GGATC	4 cut(s)	576, 637, 650, 2332
Ama87I	CYCGRG	1 cut(s)	134
AoxI	GGCC	5 cut(s)	391, 411, 484, 1804, 2532
ApoI	RAATTY	3 cut(s)	37, 1864, 2276
ArsI	GACNNNNNNTTYG	2 cut(s)	589, 621
AseI	ATTAAT	1 cut(s)	1731
Asp700I	GAANNNNTTC	1 cut(s)	2278
AspA2I	CCTAGG	3 cut(s)	620, 2015, 2428
AspLEI	GCGC	2 cut(s)	993, 1284
AspS9I	GGNCC	4 cut(s)	1219, 1532, 1590, 1672
AsuC2I	CCSGG	4 cut(s)	286, 381, 415, 1598
AsuHPI	GGTGA	4 cut(s)	536, 1051, 1249, 1612
AsuII	TTCGAA	1 cut(s)	299
AvaI	CYCGRG	1 cut(s)	134
AvaII	GGWCC	4 cut(s)	1219, 1532, 1590, 1672
AvrII	CCTAGG	3 cut(s)	620, 2015, 2428
BaeGI	GKGCMC	1 cut(s)	195
BaeI	ACNNNNGTAYC	2 cut(s)	1734, 1767
BalI	TGGCCA	1 cut(s)	393
BamHI	GGATCC	1 cut(s)	642
BanI	GGYRCC	3 cut(s)	192, 531, 1493
BanII	GRGCYC	2 cut(s)	135, 1138
BauI	CACGAG	1 cut(s)	344
BbsI	GAAGAC	2 cut(s)	956, 2004
BccI	CCATC	7 cut(s)	283, 619, 1180, 1262, 2081, 2291, 2468
BceAI	ACGGC	2 cut(s)	1791, 1985
BciT130I	CCWGG	4 cut(s)	16, 595, 884, 1223
BciVI	GTATCC	1 cut(s)	2317
BclI	TGATCA	2 cut(s)	2119, 2286
BcnI	CCSGG	4 cut(s)	286, 381, 415, 1598
BcoDI	GTCTC	6 cut(s)	275, 512, 675, 1503, 2051, 2488
BfaI	CTAG	7 cut(s)	90, 621, 1920, 2016, 2039, 2357, 2429
BfmI	CTRYAG	2 cut(s)	20, 572
BfoI	RGCGCY	1 cut(s)	994
BfuI	GTATCC	1 cut(s)	2317
BglI	GCCNNNNNGGC	1 cut(s)	419
BlnI	CCTAGG	3 cut(s)	620, 2015, 2428
BmcAI	AGTACT	1 cut(s)	2178
Bme1390I	CCNGG	8 cut(s)	16, 286, 381, 415, 595, 884, 1223, 1598
Bme18I	GGWCC	4 cut(s)	1219, 1532, 1590, 1672
BmeRI	GACNNNNNGTC	1 cut(s)	268
BmeT110I	CYCGRG	1 cut(s)	134
BmgT120I	GGNCC	4 cut(s)	1219, 1532, 1590, 1672
BmrFI	CCNGG	8 cut(s)	16, 286, 381, 415, 595, 884, 1223, 1598
BmrI	ACTGGG	2 cut(s)	574, 2508
BmsI	GCATC	5 cut(s)	715, 1363, 2014, 2197, 2529
BmuI	ACTGGG	2 cut(s)	574, 2508
BpiI	GAAGAC	2 cut(s)	956, 2004
BplI	GAGNNNNNCTC	2 cut(s)	1024, 1056
Bpu10I	CCTNAGC	1 cut(s)	1263
Bpu14I	TTCGAA	1 cut(s)	299
BpuEI	CTTGAG	1 cut(s)	1742
BpuMI	CCSGG	4 cut(s)	286, 381, 415, 1598
BsaAI	YACGTR	1 cut(s)	984
BsaBI	GATNNNNATC	1 cut(s)	2235
BsaHI	GRCGYC	1 cut(s)	599
BsaI	GGTCTC	1 cut(s)	275
BsaJI	CCNNGG	9 cut(s)	14, 57, 135, 273, 620, 701, 1596, 2015, 2428
BsaWI	WCCGGW	2 cut(s)	109, 386
BsaXI	ACNNNNNCTCC	2 cut(s)	215, 245
Bsc4I	CCNNNNNNNGG	9 cut(s)	58, 292, 386, 400, 553, 608, 626, 680, 1503
Bse118I	RCCGGY	1 cut(s)	486
Bse1I	ACTGG	4 cut(s)	569, 2225, 2371, 2514
Bse3DI	GCAATG	2 cut(s)	1779, 2150
Bse8I	GATNNNNATC	1 cut(s)	2235
BseBI	CCWGG	4 cut(s)	16, 595, 884, 1223
BseDI	CCNNGG	9 cut(s)	14, 57, 135, 273, 620, 701, 1596, 2015, 2428
BseGI	GGATG	8 cut(s)	294, 318, 630, 636, 815, 997, 1195, 2080
BseJI	GATNNNNATC	1 cut(s)	2235
BseLI	CCNNNNNNNGG	9 cut(s)	58, 292, 386, 400, 553, 608, 626, 680, 1503
BseMI	GCAATG	2 cut(s)	1779, 2150
BseMII	CTCAG	7 cut(s)	669, 705, 798, 813, 1041, 1254, 2171
BseNI	ACTGG	4 cut(s)	569, 2225, 2371, 2514
BseSI	GKGCMC	1 cut(s)	195
BseYI	CCCAGC	1 cut(s)	32
BsgI	GTGCAG	2 cut(s)	714, 1203
Bsh1236I	CGCG	3 cut(s)	1284, 1626, 1755
BshFI	GGCC	5 cut(s)	393, 413, 486, 1806, 2534
BshNI	GGYRCC	3 cut(s)	192, 531, 1493
BsiHKCI	CYCGRG	1 cut(s)	134
BslFI	GGGAC	4 cut(s)	40, 57, 517, 2407
BslI	CCNNNNNNNGG	9 cut(s)	58, 292, 386, 400, 553, 608, 626, 680, 1503
BsmAI	GTCTC	6 cut(s)	275, 512, 675, 1503, 2051, 2488
BsmBI	CGTCTC	1 cut(s)	512
BsmFI	GGGAC	4 cut(s)	40, 57, 517, 2407
BsmI	GAATGC	2 cut(s)	604, 877
BsnI	GGCC	5 cut(s)	393, 413, 486, 1806, 2534
Bso31I	GGTCTC	1 cut(s)	275
BsoBI	CYCGRG	1 cut(s)	134
Bsp119I	TTCGAA	1 cut(s)	299
Bsp1286I	GDGCHC	3 cut(s)	135, 195, 1138
Bsp1407I	TGTACA	3 cut(s)	758, 1068, 1395
BspANI	GGCC	5 cut(s)	393, 413, 486, 1806, 2534
BspCNI	CTCAG	7 cut(s)	668, 706, 799, 814, 1040, 1255, 2172
BspFNI	CGCG	3 cut(s)	1284, 1626, 1755
BspHI	TCATGA	1 cut(s)	2122
BspMAI	CTGCAG	1 cut(s)	24
BspPI	GGATC	4 cut(s)	576, 637, 650, 2332
BspT104I	TTCGAA	1 cut(s)	299
BspT107I	GGYRCC	3 cut(s)	192, 531, 1493
BspTNI	GGTCTC	1 cut(s)	275
BsrDI	GCAATG	2 cut(s)	1779, 2150
BsrFI	RCCGGY	1 cut(s)	486
BsrGI	TGTACA	3 cut(s)	758, 1068, 1395
BsrI	ACTGG	4 cut(s)	569, 2225, 2371, 2514
BssAI	RCCGGY	1 cut(s)	486
BssECI	CCNNGG	9 cut(s)	14, 57, 135, 273, 620, 701, 1596, 2015, 2428
BssNI	GRCGYC	1 cut(s)	599
BssSI	CACGAG	1 cut(s)	344
BssT1I	CCWWGG	5 cut(s)	273, 620, 701, 2015, 2428
Bst2BI	CACGAG	1 cut(s)	344
Bst2UI	CCWGG	4 cut(s)	16, 595, 884, 1223
Bst4CI	ACNGT	8 cut(s)	861, 890, 1543, 1582, 1666, 2116, 2329, 2415
Bst6I	CTCTTC	1 cut(s)	95
BstACI	GRCGYC	1 cut(s)	599
BstAUI	TGTACA	3 cut(s)	758, 1068, 1395
BstBAI	YACGTR	1 cut(s)	984
BstBI	TTCGAA	1 cut(s)	299
BstC8I	GCNNGC	5 cut(s)	420, 488, 1307, 1908, 1974
BstF5I	GGATG	8 cut(s)	294, 318, 630, 636, 815, 997, 1195, 2080
BstFNI	CGCG	3 cut(s)	1284, 1626, 1755
BstH2I	RGCGCY	1 cut(s)	994
BstHHI	GCGC	2 cut(s)	993, 1284
BstMAI	GTCTC	6 cut(s)	275, 512, 675, 1503, 2051, 2488
BstNI	CCWGG	4 cut(s)	16, 595, 884, 1223
BstNSI	RCATGY	3 cut(s)	1069, 1309, 1516
BstSCI	CCNGG	8 cut(s)	14, 284, 379, 413, 593, 882, 1221, 1596
BstSFI	CTRYAG	2 cut(s)	20, 572
BstSLI	GKGCMC	1 cut(s)	195
BstUI	CGCG	3 cut(s)	1284, 1626, 1755
BstV2I	GAAGAC	2 cut(s)	956, 2004
BstX2I	RGATCY	3 cut(s)	568, 642, 2337
BstYI	RGATCY	3 cut(s)	568, 642, 2337
BsuI	GTATCC	1 cut(s)	2317
BsuRI	GGCC	5 cut(s)	393, 413, 486, 1806, 2534
BtgZI	GCGATG	1 cut(s)	2310
BtsCI	GGATG	8 cut(s)	294, 318, 630, 636, 815, 997, 1195, 2080
BtsI	GCAGTG	2 cut(s)	585, 1842
BtsIMutI	CAGTG	6 cut(s)	585, 866, 1245, 1539, 1842, 2232
Cac8I	GCNNGC	5 cut(s)	420, 488, 1307, 1908, 1974
CciI	TCATGA	1 cut(s)	2122
CfoI	GCGC	2 cut(s)	993, 1284
Cfr10I	RCCGGY	1 cut(s)	486
Cfr13I	GGNCC	4 cut(s)	1219, 1532, 1590, 1672
CseI	GACGC	2 cut(s)	607, 1273
CsiI	ACCWGGT	1 cut(s)	1221
Csp6I	GTAC	5 cut(s)	759, 1069, 1396, 2163, 2177
CspCI	CAANNNNNGTGG	2 cut(s)	846, 881
CviAII	CATG	9 cut(s)	175, 1066, 1306, 1513, 1528, 1645, 1717, 2123, 2498
CviQI	GTAC	5 cut(s)	759, 1069, 1396, 2163, 2177
DrdI	GACNNNNNNGTC	1 cut(s)	893
DriI	GACNNNNNGTC	1 cut(s)	268
DseDI	GACNNNNNNGTC	1 cut(s)	893
EaeI	YGGCCR	4 cut(s)	391, 484, 1804, 2532
Eam1104I	CTCTTC	1 cut(s)	95
Eam1105I	GACNNNNNGTC	1 cut(s)	268
EarI	CTCTTC	1 cut(s)	95
EciI	GGCGGA	3 cut(s)	178, 366, 446
Eco130I	CCWWGG	5 cut(s)	273, 620, 701, 2015, 2428
Eco24I	GRGCYC	2 cut(s)	135, 1138
Eco31I	GGTCTC	1 cut(s)	275
Eco47I	GGWCC	4 cut(s)	1219, 1532, 1590, 1672
Eco57I	CTGAAG	1 cut(s)	975
Eco88I	CYCGRG	1 cut(s)	134
EcoO109I	RGGNCCY	1 cut(s)	1532
EcoRI	GAATTC	1 cut(s)	37
EcoRII	CCWGG	4 cut(s)	14, 593, 882, 1221
EcoT14I	CCWWGG	5 cut(s)	273, 620, 701, 2015, 2428
EcoT22I	ATGCAT	1 cut(s)	1275
EcoT38I	GRGCYC	2 cut(s)	135, 1138
ErhI	CCWWGG	5 cut(s)	273, 620, 701, 2015, 2428
Esp3I	CGTCTC	1 cut(s)	512
FaeI	CATG	9 cut(s)	178, 1069, 1309, 1516, 1531, 1648, 1720, 2126, 2501
FalI	AAGNNNNNCTT	6 cut(s)	1890, 1922, 2152, 2184, 2354, 2386
FaqI	GGGAC	4 cut(s)	40, 57, 517, 2407
FatI	CATG	9 cut(s)	174, 1065, 1305, 1512, 1527, 1644, 1716, 2122, 2497
FauI	CCCGC	4 cut(s)	286, 411, 2020, 2432
FbaI	TGATCA	2 cut(s)	2119, 2286
FokI	GGATG	8 cut(s)	301, 325, 637, 643, 802, 984, 1182, 2087
FriOI	GRGCYC	2 cut(s)	135, 1138
FspBI	CTAG	7 cut(s)	90, 621, 1920, 2016, 2039, 2357, 2429
GlaI	GCGC	2 cut(s)	992, 1283
GsaI	CCCAGC	1 cut(s)	36
HaeII	RGCGCY	1 cut(s)	994
HaeIII	GGCC	5 cut(s)	393, 413, 486, 1806, 2534
HgaI	GACGC	2 cut(s)	607, 1273
HhaI	GCGC	2 cut(s)	993, 1284
Hin1I	GRCGYC	1 cut(s)	599
Hin1II	CATG	9 cut(s)	178, 1069, 1309, 1516, 1531, 1648, 1720, 2126, 2501
Hin6I	GCGC	2 cut(s)	991, 1282
HinP1I	GCGC	2 cut(s)	991, 1282
HincII	GTYRAC	1 cut(s)	2203
HindII	GTYRAC	1 cut(s)	2203
HindIII	AAGCTT	2 cut(s)	1126, 1904
HphI	GGTGA	4 cut(s)	536, 1051, 1249, 1612
Hpy166II	GTNNAC	4 cut(s)	1593, 1672, 2165, 2203
Hpy188III	TCNNGA	8 cut(s)	42, 320, 609, 929, 1535, 1895, 2034, 2123
Hpy8I	GTNNAC	4 cut(s)	1593, 1672, 2165, 2203
Hpy99I	CGWCG	1 cut(s)	2540
HpyAV	CCTTC	9 cut(s)	220, 253, 436, 447, 558, 793, 1004, 1320, 2178
HpyCH4III	ACNGT	8 cut(s)	861, 890, 1543, 1582, 1666, 2116, 2329, 2415
HpyCH4IV	ACGT	3 cut(s)	521, 983, 1180
HpySE526I	ACGT	3 cut(s)	521, 983, 1180
Hsp92I	GRCGYC	1 cut(s)	599
Hsp92II	CATG	9 cut(s)	178, 1069, 1309, 1516, 1531, 1648, 1720, 2126, 2501
HspAI	GCGC	2 cut(s)	991, 1282
KroI	GCCGGC	1 cut(s)	486
KroNI	GCCGGC	1 cut(s)	488
Ksp22I	TGATCA	2 cut(s)	2119, 2286
LmnI	GCTCC	8 cut(s)	93, 130, 349, 368, 389, 1045, 1279, 1499
LweI	GCATC	5 cut(s)	715, 1363, 2014, 2197, 2529
MabI	ACCWGGT	1 cut(s)	1221
MaeI	CTAG	7 cut(s)	90, 621, 1920, 2016, 2039, 2357, 2429
MaeII	ACGT	3 cut(s)	521, 983, 1180
MaeIII	GTNAC	4 cut(s)	142, 1243, 1983, 2434
MfeI	CAATTG	4 cut(s)	911, 1142, 1358, 1462
MflI	RGATCY	3 cut(s)	568, 642, 2337
MhlI	GDGCHC	3 cut(s)	135, 195, 1138
MlsI	TGGCCA	1 cut(s)	393
MluNI	TGGCCA	1 cut(s)	393
MlyI	GAGTC	4 cut(s)	812, 833, 2456, 2457
MmeI	TCCRAC	2 cut(s)	1126, 2434
Mox20I	TGGCCA	1 cut(s)	393
Mph1103I	ATGCAT	1 cut(s)	1275
MroNI	GCCGGC	1 cut(s)	486
MroXI	GAANNNNTTC	1 cut(s)	2278
MscI	TGGCCA	1 cut(s)	393
MseI	TTAA	7 cut(s)	66, 474, 1614, 1731, 1937, 2097, 2543
MslI	CAYNNNNRTG	4 cut(s)	1064, 1598, 2127, 2525
Msp20I	TGGCCA	1 cut(s)	393
MspA1I	CMGCKG	1 cut(s)	32
MspR9I	CCNGG	8 cut(s)	16, 286, 381, 415, 595, 884, 1223, 1598
MunI	CAATTG	4 cut(s)	911, 1142, 1358, 1462
Mva1269I	GAATGC	2 cut(s)	604, 877
MvaI	CCWGG	4 cut(s)	16, 595, 884, 1223
MvnI	CGCG	3 cut(s)	1284, 1626, 1755
NaeI	GCCGGC	1 cut(s)	488
NciI	CCSGG	4 cut(s)	286, 381, 415, 1598
NgoMIV	GCCGGC	1 cut(s)	486
NlaIII	CATG	9 cut(s)	178, 1069, 1309, 1516, 1531, 1648, 1720, 2126, 2501
NmeAIII	GCCGAG	1 cut(s)	82
NmuCI	GTSAC	3 cut(s)	142, 1243, 2434
NsiI	ATGCAT	1 cut(s)	1275
NspI	RCATGY	3 cut(s)	1069, 1309, 1516
NspV	TTCGAA	1 cut(s)	299
OliI	CACNNNNGTG	1 cut(s)	1598
PaeI	GCATGC	1 cut(s)	1309
PagI	TCATGA	1 cut(s)	2122
PciI	ACATGT	1 cut(s)	1512
PctI	GAATGC	2 cut(s)	604, 877
PdiI	GCCGGC	1 cut(s)	488
PdmI	GAANNNNTTC	1 cut(s)	2278
PflMI	CCANNNNNTGG	1 cut(s)	400
PleI	GAGTC	4 cut(s)	811, 832, 2455, 2457
PpsI	GAGTC	4 cut(s)	811, 832, 2455, 2457
Ppu21I	YACGTR	1 cut(s)	984
PpuMI	RGGWCCY	1 cut(s)	1532
PscI	ACATGT	1 cut(s)	1512
PshBI	ATTAAT	1 cut(s)	1731
Psp5II	RGGWCCY	1 cut(s)	1532
Psp6I	CCWGG	4 cut(s)	14, 593, 882, 1221
PspFI	CCCAGC	1 cut(s)	32
PspGI	CCWGG	4 cut(s)	14, 593, 882, 1221
PspPI	GGNCC	4 cut(s)	1219, 1532, 1590, 1672
PspPPI	RGGWCCY	1 cut(s)	1532
PstI	CTGCAG	1 cut(s)	24
PsuI	RGATCY	3 cut(s)	568, 642, 2337
PvuII	CAGCTG	1 cut(s)	32
RsaI	GTAC	5 cut(s)	760, 1070, 1397, 2164, 2178
RsaNI	GTAC	5 cut(s)	759, 1069, 1396, 2163, 2177
RseI	CAYNNNNRTG	4 cut(s)	1064, 1598, 2127, 2525
SaqAI	TTAA	7 cut(s)	66, 474, 1614, 1731, 1937, 2097, 2543
Sau96I	GGNCC	4 cut(s)	1219, 1532, 1590, 1672
ScaI	AGTACT	1 cut(s)	2178
SchI	GAGTC	4 cut(s)	812, 833, 2456, 2457
ScrFI	CCNGG	8 cut(s)	16, 286, 381, 415, 595, 884, 1223, 1598
SduI	GDGCHC	3 cut(s)	135, 195, 1138
SexAI	ACCWGGT	1 cut(s)	1221
SfaNI	GCATC	5 cut(s)	715, 1363, 2014, 2197, 2529
SfcI	CTRYAG	2 cut(s)	20, 572
SfuI	TTCGAA	1 cut(s)	299
SinI	GGWCC	4 cut(s)	1219, 1532, 1590, 1672
SmiMI	CAYNNNNRTG	4 cut(s)	1064, 1598, 2127, 2525
SmlI	CTYRAG	1 cut(s)	1721
SmoI	CTYRAG	1 cut(s)	1721
SphI	GCATGC	1 cut(s)	1309
SspI	AATATT	1 cut(s)	246
SspMI	CTAG	7 cut(s)	90, 621, 1920, 2016, 2039, 2357, 2429
StyD4I	CCNGG	8 cut(s)	14, 284, 379, 413, 593, 882, 1221, 1596
StyI	CCWWGG	5 cut(s)	273, 620, 701, 2015, 2428
TaaI	ACNGT	8 cut(s)	861, 890, 1543, 1582, 1666, 2116, 2329, 2415
TaiI	ACGT	3 cut(s)	524, 986, 1183
TaqI	TCGA	4 cut(s)	207, 299, 2046, 2466
TaqII	GACCGA	1 cut(s)	1689
TatI	WGTACW	5 cut(s)	758, 1068, 1395, 2162, 2176
TauI	GCSGC	3 cut(s)	425, 604, 1887
Tru1I	TTAA	7 cut(s)	66, 474, 1614, 1731, 1937, 2097, 2543
Tru9I	TTAA	7 cut(s)	66, 474, 1614, 1731, 1937, 2097, 2543
TscAI	CASTG	6 cut(s)	585, 866, 1252, 1546, 1849, 2232
TseFI	GTSAC	3 cut(s)	142, 1243, 2434
Tsp45I	GTSAC	3 cut(s)	142, 1243, 2434
TspGWI	ACGGA	7 cut(s)	143, 575, 663, 748, 1232, 1936, 2553
TspRI	CASTG	6 cut(s)	585, 866, 1252, 1546, 1849, 2232
Van91I	CCANNNNNTGG	1 cut(s)	400
VpaK11BI	GGWCC	4 cut(s)	1219, 1532, 1590, 1672
VspI	ATTAAT	1 cut(s)	1731
XapI	RAATTY	3 cut(s)	37, 1864, 2276
XceI	RCATGY	3 cut(s)	1069, 1309, 1516
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1468
XmaJI	CCTAGG	3 cut(s)	620, 2015, 2428
XmnI	GAANNNNTTC	1 cut(s)	2278
XspI	CTAG	7 cut(s)	90, 621, 1920, 2016, 2039, 2357, 2429
ZrmI	AGTACT	1 cut(s)	2178
Zsp2I	ATGCAT	1 cut(s)	1275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.