Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0013028

Physical Location & Seq

Genomic Coordinates Forward (+)

33122571 .. 33127163

Gene Structure 4593 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1261900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3195 bp

ATGAGAGCGGGGCTGAGTACGATCCAGCAAACGTTAACCCCAGAGGCGGCAAGCGTCTTGAATCACTCGATTGCCGAAGCGGGTCGACGGAACCACGGCCAGACGACGCCGCTACACGTGGCAGCAACCCTTTTGTCCTCCCCCACCGGCTTCCTCCGCCAAGCATGCATCAAATCTCACCCCAATTCCTCCCACCCTCTGCAGTGCCGAGCTCTGGAGCTCTGCTTCAGTGTGGCTCTGGAGCGGTTGCCCACCGCCCAAAACATGAGTCCCGGCATGGAGCCCCCCATCTCTAATGCTCTCATGGCCGCCTTGAAGCGAGCTCAAGCTCACCAGCGCCGCGGCTGCCCCGAACAGCAGCAGCAACCTCTATTAGCCGTCAAAGTCGAGCTCGAGCAGCTCATCATCTCAATTCTCGATGACCCGAGTGTTAGTCGGGTCATGCGGGAGGCTAGTTTCTCTAGTCCCGCAGTCAAAGCCACCATTGAACAGACGCTCAACTCTTCTGCCGCCGCCGCCGCTCACGCCGCCGTCAATTCATCCCCAATCGGATTGCAATTCCGCCCTGCAGGCCCCATGGTGCCACCCGTGAGCCGGAATTTGTATTTGAATCCGAGGCTGCAGCAGCCGCAGGGAGCTGCAACACAATCGGTACAACATAGAGGGGAAGAGGTTAAGAGGGTTGCTGATATTTTGTTGAGAACGAAGAAGAGGAACCCAGTTTTGGTTGGCGATTCGGAGCCTGAGGCCGTGACCAAAGAGCTCTTGCGGAGGATTCAGAGCAAGGAGTTGGGTGAGGGGCCGCTGAAGAATGTTGATGTCCTTCATTTGGAGGAGGTTGTTTCTTTGGATAGGAATCAGATAGTGTCAAAAATGAAGGAATTAGGGGGTTTGATTGAGACCCGGTTGTTGAATTTGACCGGGGGAGGGGTCATTCTTGATTTGGGTGACTTGAAATGGCTTGTGGAGCAGCCTGCGAGCTTTGGAGGAGTTCCGGGTCTAGTTTCGTCTCCCGTCCAACAGCAGGTGGTTTCGGAGGCAGGGCGGGCAGCAGTGGGGGAAATGGGGAAGCTATTGGCGAGGTATGGAGAGGGTAGTGCAACTGGTGGCCGGCTTTGGTTAATTGGGACTGCTACTTGTGAGACCTATTTGAGGTGCCAAGTCTATCACCCTTCAATGGAAACTGATTGGGATCTACAGGCAGTGCCAATTGCTGGCAGAACGCCACTTTCAGGATTGTTTCCGAGGATTGGGGCGACCAACGGGATCCTTAGTAGCTCAGTTGAATCTTTGTCACCAATGAAAGGTTTTCCATCGGCATCAATTCCTCAACCAAGACTTTTGTCAGAGAACTCGGATCCTGCTCGAAGAGAAACATGTTGCCCGCAATGTACAGAGAGTTATGAGCAAGAGCTTGCAAAACTGGTTGCCAAGGAATCTGAGAAATCATCTTCTGAATCAGACGCAGCTCAACCACCGCTACCTCAGTGGTTGCAGAACGCTAAACCCCGTGATGTTCATGCCAGTACATTAGATCAGACGCAGACTACGGACCAAAATTTGATCCTGAATCAAAGGACTAATGAATTACAGAAGGAATGGAGAGATACATGTTTGCGTCTTCATCCTAATTTTCATCAGCCCAGTTTCAGCTTGAAGAGAATTATTCCAACAACCCTCTCAATGACGGGCTTGTACAATCCAAACTTGCTTGGTCACCAACCATTCCAAACCAGATCACATGTGAATAAGAATCTTGGGACTCTGCAATTGAACACAAATCCGCTGACCAGCCAACCATCTGAACGGGCAATTTCCCAACCAGAAAGCCCTGTAAGGACAGAGCTGGTTCTTGGTCAAACAGAAGTCACTGAAATCAACTCCAAGCAAACGCATAAAGAGCGCATCAGAGACTTTATGGGCTGCATGCCTTCTGAACCACAGAACAAGTTACATGAAATGCAAACGGAGGATAAACAATTGTGTCAAATAGATACTGATTCATTCAAAAAGCTGTACAAAGGTTTAATGGAGGTGTGGTGGCAGCAAGAAGCAGCAGCCTCTGTGGCTGAAACTGTGACACAATGCAAGTTGGGCAATGGAAAGAGACGTCGTGCTGGGTCAAGGGGAGACATGTGGCTGCTGTTTATGGGCCTGGACAGTGTTGGCAAGAAGAAGATGGCATCAGCACTTTCAGAACTGGTATGTGGGTCCAACCCAGTGATGATCAGTCTTAGCTCCCAACGTGGTAATTTGCAATCAGATATGAGTTTCCGTGGTAAAACAGTGGTGGATCGAATAGCAGAAACAGTTAAGAGAAACCCTTTTTCAGTAGTTGTGCTTGAGGACATTAATGAAGCTGATCTGATAGTTCGTGGTAGCATAAAACAGGCAATTGAGAGAGGTCGTCTTGCTGACTCTTATGGCCGTGAAATCAGTCTTGGAAATGTTATTTTCATACTTACCGCAAATTGGTTGCCAGAAAATCTTAGGCCCTTGTCAAATGACAATTCACTTGAAGAAAAACTCGCCAGTATTGCGAGGAGCAGTTGGCAGTTAAAGCTATCGGTCTGTGCTAGGGCTGCAAAACGCCATGCCAATTGGTTAACAGATGAAGACAGGGCCACAAAGCCTAGGACAGATACTGGTTCAGCATTAGGATTCGATCTGAATGAAGCTGCGGATGCTGAGGATGACAGAACAGACGGCTCGCTTAATTCAAGTGATCTTACGGTTGATAATGAAGATGACAATCGTCTCAATAACAGAACCTTACTCAAAGTCACAACCGCATCAGTACCCCGGGAATTACTGGATAGTGTTGATGATGCCATTGTGTTCAAGCCAGTGGATTTCAATCCCATTCGGCAAAACATCACAAACTCCATCAAAAAAAGATTCTCAAAAATAATGGGTGAGGGGATTTCATTTGAATTACCAGAGGATGCTGTTGAAAAGATCCTGACCGGGATATGGCTAGGCCGGACTGGCTTAGAGGAATGGGCAGAAAAGGTTCTCGCCCCGAGCATCCAGCAACTCAAGTCCTACCTTGGTGGCAGCACTGGTGTGATAGCCGACGAGTCATTGGTGGTGAGGCTTGAATCTGATGGTGCTTCGGACGACCGGAGCCCTGGAGATAGGCTCCCTAGTAGCATCAATGTGGGTGTGGTGCCTGATGGGTTGAGACAACGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007275 GO:0007623 GO:0008150 GO:0009628 GO:0009719 GO:0009725 GO:0009791 GO:0009845 GO:0010033 GO:0014070 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0048511 GO:0048856 GO:0050896 GO:0080167 GO:0090351 GO:1901700 GO:1902347

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_2 Clp_N

Protein Analysis

1065

Amino Acids

116.62

Weight (kDa)

6.57

Isoelectric Point (pI)

48.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	12 - 113	1.1e-06	Clp repeat (R) N-terminal domain
NBD_SMAX1	PF23569	221 - 405	2.6e-32	SMAX1 nucleotide binding domain
AAA_2	PF07724	767 - 831	4.3e-08	AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1	PF26587	951 - 1037	3.2e-23	SMAX1-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57710
fragaria_vesca	FvH4_4g09820
malus_domestica	MD13G1261900.v1.1 MD16G1264200.v1.1
prunus_persica	Prupe.1G095200_v2.0.a1 Prupe.1G095200_v2.0.a1
pyrus_communis	pycom13g24720 pycom16g23290
rosa_chinensis	RchiOBHm_Chr4g0404601
rosa_laevigata	RLG00000008920
rosa_multiflora	Rmu_sc0002699.1_g000006
rosa_roxburghii	Rroxscaffold_5G00348750
rosa_rugosa	Rorug04G0047700
rosa_samantha	Rh4AG123800 Rh4BG117200 Rh4CG131900 Rh4DG116700
rosa_wichuraiana	Rw4G010030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1015
AatII	GACGTC	1 cut(s)	2113
Acc36I	ACCTGC	1 cut(s)	1015
AccB1I	GGYRCC	3 cut(s)	580, 1155, 3169
AccB7I	CCANNNNNTGG	1 cut(s)	1214
AccBSI	CCGCTC	3 cut(s)	8, 244, 521
AccI	GTMKAC	1 cut(s)	85
AccII	CGCG	1 cut(s)	342
AclI	AACGTT	1 cut(s)	32
AclWI	GGATC	9 cut(s)	16, 1200, 1261, 1274, 1352, 1365, 1558, 2301, 2953
AcoI	YGGCCR	4 cut(s)	97, 306, 1108, 2425
AcsI	RAATTY	3 cut(s)	598, 913, 1558
AcuI	CTGAAG	2 cut(s)	211, 827
AcvI	CACGTG	1 cut(s)	118
AcyI	GRCGYC	2 cut(s)	107, 2110
AfaI	GTAC	7 cut(s)	19, 654, 1393, 1528, 1697, 2018, 2799
AflIII	ACRYGT	5 cut(s)	115, 1376, 1610, 1741, 2133
AjnI	CCWGG	2 cut(s)	2154, 3130
AjuI	GAANNNNNNNTTGG	4 cut(s)	707, 739, 2424, 2456
AleI	CACNNNNGTG	1 cut(s)	3065
Alw21I	GWGCWC	5 cut(s)	214, 222, 325, 393, 765
Alw26I	GTCTC	8 cut(s)	893, 1014, 1136, 1905, 2101, 2124, 2762, 3178
AlwI	GGATC	9 cut(s)	16, 1200, 1261, 1274, 1352, 1365, 1558, 2301, 2953
AlwNI	CAGNNNCTG	2 cut(s)	2063, 2645
Ama87I	CYCGRG	4 cut(s)	392, 424, 2802, 3022
ApoI	RAATTY	3 cut(s)	598, 913, 1558
ArsI	GACNNNNNNTTYG	2 cut(s)	253, 285
AseI	ATTAAT	1 cut(s)	2352
Asp700I	GAANNNNTTC	1 cut(s)	3012
AspA2I	CCTAGG	1 cut(s)	2633
AspLEI	GCGC	2 cut(s)	339, 1905
AspS9I	GGNCC	7 cut(s)	572, 800, 1552, 2152, 2211, 2493, 2622
AsuC2I	CCSGG	7 cut(s)	273, 904, 922, 996, 2803, 2804, 2968
AsuHPI	GGTGA	9 cut(s)	170, 323, 806, 959, 1160, 1287, 1709, 2927, 3103
AvaI	CYCGRG	4 cut(s)	392, 424, 2802, 3022
AvaII	GGWCC	2 cut(s)	1552, 2211
AvrII	CCTAGG	1 cut(s)	2633
AxyI	CCTNAGG	1 cut(s)	744
BamHI	GGATCC	2 cut(s)	1266, 1357
BanI	GGYRCC	3 cut(s)	580, 1155, 3169
BanII	GRGCYC	7 cut(s)	214, 222, 285, 325, 393, 765, 3131
BbrPI	CACGTG	1 cut(s)	118
BbsI	GAAGAC	2 cut(s)	1613, 2622
Bbv12I	GWGCWC	5 cut(s)	214, 222, 325, 393, 765
BbvCI	CCTCAGC	1 cut(s)	2688
BccI	CCATC	7 cut(s)	296, 1321, 1807, 2173, 2894, 3101, 3170
BceAI	ACGGC	6 cut(s)	112, 362, 515, 734, 2412, 2722
BcgI	CGANNNNNNTGC	2 cut(s)	630, 664
BciT130I	CCWGG	2 cut(s)	2156, 3132
BclI	TGATCA	1 cut(s)	2226
BcnI	CCSGG	7 cut(s)	273, 904, 922, 996, 2803, 2804, 2968
BcoDI	GTCTC	8 cut(s)	893, 1014, 1136, 1905, 2101, 2124, 2762, 3178
BfaI	CTAG	7 cut(s)	453, 462, 1001, 2577, 2634, 2978, 3147
BfmI	CTRYAG	4 cut(s)	200, 567, 620, 1196
BfoI	RGCGCY	1 cut(s)	340
BfuAI	ACCTGC	1 cut(s)	1015
BglI	GCCNNNNNGGC	3 cut(s)	570, 2066, 2988
BlnI	CCTAGG	1 cut(s)	2633
Bme1390I	CCNGG	9 cut(s)	273, 904, 922, 996, 2156, 2803, 2804, 2968, 3132
Bme18I	GGWCC	2 cut(s)	1552, 2211
BmeT110I	CYCGRG	4 cut(s)	392, 424, 2802, 3022
BmgT120I	GGNCC	7 cut(s)	572, 800, 1552, 2152, 2211, 2493, 2622
BmrFI	CCNGG	9 cut(s)	273, 904, 922, 996, 2156, 2803, 2804, 2968, 3132
BmrI	ACTGGG	3 cut(s)	713, 1638, 2213
BmuI	ACTGGG	3 cut(s)	713, 1638, 2213
BoxI	GACNNNNGTC	2 cut(s)	1342, 2754
BpiI	GAAGAC	2 cut(s)	1613, 2622
BplI	GAGNNNNNCTC	2 cut(s)	3126, 3158
BpmI	CTGGAG	3 cut(s)	236, 260, 3153
Bpu10I	CCTNAGC	1 cut(s)	2688
BpuEI	CTTGAG	3 cut(s)	309, 2363, 3023
BpuMI	CCSGG	7 cut(s)	273, 904, 922, 996, 2803, 2804, 2968
BsaAI	YACGTR	1 cut(s)	118
BsaBI	GATNNNNATC	4 cut(s)	855, 1191, 2751, 2856
BsaHI	GRCGYC	2 cut(s)	107, 2110
BsaI	GGTCTC	2 cut(s)	893, 1136
BsaWI	WCCGGW	1 cut(s)	3123
BsaXI	ACNNNNNCTCC	2 cut(s)	2024, 2054
Bse118I	RCCGGY	2 cut(s)	146, 1110
Bse21I	CCTNAGG	1 cut(s)	744
Bse3DI	GCAATG	2 cut(s)	1394, 2104
Bse8I	GATNNNNATC	4 cut(s)	855, 1191, 2751, 2856
BseBI	CCWGG	2 cut(s)	2156, 3132
BseGI	GGATG	6 cut(s)	539, 1624, 2689, 2698, 2950, 3027
BseJI	GATNNNNATC	4 cut(s)	855, 1191, 2751, 2856
BseMI	GCAATG	2 cut(s)	1394, 2104
BseMII	CTCAG	6 cut(s)	5, 735, 1293, 1431, 1499, 2679
BseRI	GAGGAG	3 cut(s)	848, 1002, 2557
BseYI	CCCAGC	1 cut(s)	2117
Bsh1236I	CGCG	1 cut(s)	342
Bsh1285I	CGRYCG	1 cut(s)	3124
BshNI	GGYRCC	3 cut(s)	580, 1155, 3169
BsiEI	CGRYCG	1 cut(s)	3124
BsiHKAI	GWGCWC	5 cut(s)	214, 222, 325, 393, 765
BsiHKCI	CYCGRG	4 cut(s)	392, 424, 2802, 3022
BslFI	GGGAC	4 cut(s)	255, 450, 1141, 1774
BsmAI	GTCTC	8 cut(s)	893, 1014, 1136, 1905, 2101, 2124, 2762, 3178
BsmBI	CGTCTC	3 cut(s)	1014, 2101, 2762
BsmFI	GGGAC	4 cut(s)	255, 450, 1141, 1774
Bso31I	GGTCTC	2 cut(s)	893, 1136
BsoBI	CYCGRG	4 cut(s)	392, 424, 2802, 3022
Bsp1286I	GDGCHC	7 cut(s)	214, 222, 285, 325, 393, 765, 3131
Bsp1407I	TGTACA	3 cut(s)	1391, 1695, 2016
Bsp19I	CCATGG	1 cut(s)	576
BspCNI	CTCAG	6 cut(s)	6, 736, 1292, 1432, 1498, 2680
BspFNI	CGCG	1 cut(s)	342
BspMAI	CTGCAG	3 cut(s)	204, 571, 624
BspMI	ACCTGC	1 cut(s)	1015
BspPI	GGATC	9 cut(s)	16, 1200, 1261, 1274, 1352, 1365, 1558, 2301, 2953
BspT107I	GGYRCC	3 cut(s)	580, 1155, 3169
BspTNI	GGTCTC	2 cut(s)	893, 1136
BsrBI	CCGCTC	3 cut(s)	8, 244, 521
BsrDI	GCAATG	2 cut(s)	1394, 2104
BsrFI	RCCGGY	2 cut(s)	146, 1110
BsrGI	TGTACA	3 cut(s)	1391, 1695, 2016
BssAI	RCCGGY	2 cut(s)	146, 1110
BssNI	GRCGYC	2 cut(s)	107, 2110
BssT1I	CCWWGG	4 cut(s)	576, 1431, 2633, 3049
Bst2UI	CCWGG	2 cut(s)	2156, 3132
Bst4CI	ACNGT	5 cut(s)	2077, 2162, 2287, 2311, 2734
Bst6I	CTCTTC	5 cut(s)	508, 663, 704, 1363, 1652
BstACI	GRCGYC	2 cut(s)	107, 2110
BstAUI	TGTACA	3 cut(s)	1391, 1695, 2016
BstBAI	YACGTR	1 cut(s)	118
BstDSI	CCRYGG	4 cut(s)	94, 340, 576, 2275
BstEII	GGTNACC	1 cut(s)	1715
BstENI	CCTNNNNNAGG	1 cut(s)	1150
BstF5I	GGATG	6 cut(s)	539, 1624, 2689, 2698, 2950, 3027
BstFNI	CGCG	1 cut(s)	342
BstH2I	RGCGCY	1 cut(s)	340
BstHHI	GCGC	2 cut(s)	339, 1905
BstMAI	GTCTC	8 cut(s)	893, 1014, 1136, 1905, 2101, 2124, 2762, 3178
BstMCI	CGRYCG	1 cut(s)	3124
BstNI	CCWGG	2 cut(s)	2156, 3132
BstNSI	RCATGY	6 cut(s)	168, 1380, 1614, 1745, 1930, 2137
BstPAI	GACNNNNGTC	2 cut(s)	1342, 2754
BstPI	GGTNACC	1 cut(s)	1715
BstSCI	CCNGG	9 cut(s)	271, 902, 920, 994, 2154, 2801, 2802, 2966, 3130
BstSFI	CTRYAG	4 cut(s)	200, 567, 620, 1196
BstUI	CGCG	1 cut(s)	342
BstV2I	GAAGAC	2 cut(s)	1613, 2622
BstX2I	RGATCY	4 cut(s)	1192, 1266, 1357, 2958
BstYI	RGATCY	4 cut(s)	1192, 1266, 1357, 2958
Bsu36I	CCTNAGG	1 cut(s)	744
BtgI	CCRYGG	4 cut(s)	94, 340, 576, 2275
BtsCI	GGATG	6 cut(s)	539, 1624, 2689, 2698, 2950, 3027
BtsI	GCAGTG	3 cut(s)	209, 1059, 1209
BveI	ACCTGC	1 cut(s)	1015
CaiI	CAGNNNCTG	2 cut(s)	2063, 2645
CfoI	GCGC	2 cut(s)	339, 1905
Cfr10I	RCCGGY	2 cut(s)	146, 1110
Cfr13I	GGNCC	7 cut(s)	572, 800, 1552, 2152, 2211, 2493, 2622
Cfr42I	CCGCGG	1 cut(s)	343
Cfr9I	CCCGGG	1 cut(s)	2802
CseI	GACGC	6 cut(s)	43, 115, 502, 1472, 1549, 1607
Csp6I	GTAC	7 cut(s)	18, 653, 1392, 1527, 1696, 2017, 2798
CviQI	GTAC	7 cut(s)	18, 653, 1392, 1527, 1696, 2017, 2798
EaeI	YGGCCR	4 cut(s)	97, 306, 1108, 2425
Eam1104I	CTCTTC	5 cut(s)	508, 663, 704, 1363, 1652
EarI	CTCTTC	5 cut(s)	508, 663, 704, 1363, 1652
EciI	GGCGGA	2 cut(s)	146, 551
Ecl136II	GAGCTC	5 cut(s)	212, 220, 323, 391, 763
Eco130I	CCWWGG	4 cut(s)	576, 1431, 2633, 3049
Eco24I	GRGCYC	7 cut(s)	214, 222, 285, 325, 393, 765, 3131
Eco31I	GGTCTC	2 cut(s)	893, 1136
Eco47I	GGWCC	2 cut(s)	1552, 2211
Eco53kI	GAGCTC	5 cut(s)	212, 220, 323, 391, 763
Eco57I	CTGAAG	2 cut(s)	211, 827
Eco72I	CACGTG	1 cut(s)	118
Eco81I	CCTNAGG	1 cut(s)	744
Eco88I	CYCGRG	4 cut(s)	392, 424, 2802, 3022
Eco91I	GGTNACC	1 cut(s)	1715
EcoICRI	GAGCTC	5 cut(s)	212, 220, 323, 391, 763
EcoNI	CCTNNNNNAGG	1 cut(s)	1150
EcoO109I	RGGNCCY	2 cut(s)	572, 2493
EcoO65I	GGTNACC	1 cut(s)	1715
EcoRII	CCWGG	2 cut(s)	2154, 3130
EcoT14I	CCWWGG	4 cut(s)	576, 1431, 2633, 3049
EcoT22I	ATGCAT	1 cut(s)	170
EcoT38I	GRGCYC	7 cut(s)	214, 222, 285, 325, 393, 765, 3131
ErhI	CCWWGG	4 cut(s)	576, 1431, 2633, 3049
Esp3I	CGTCTC	3 cut(s)	1014, 2101, 2762
FaqI	GGGAC	4 cut(s)	255, 450, 1141, 1774
FauI	CCCGC	5 cut(s)	73, 438, 475, 1038, 1392
FbaI	TGATCA	1 cut(s)	2226
FblI	GTMKAC	1 cut(s)	85
FokI	GGATG	6 cut(s)	526, 1611, 2696, 2705, 2957, 3014
FriOI	GRGCYC	7 cut(s)	214, 222, 285, 325, 393, 765, 3131
FspBI	CTAG	7 cut(s)	453, 462, 1001, 2577, 2634, 2978, 3147
GlaI	GCGC	2 cut(s)	338, 1904
GsaI	CCCAGC	1 cut(s)	2121
GsuI	CTGGAG	3 cut(s)	236, 260, 3153
HaeII	RGCGCY	1 cut(s)	340
HgaI	GACGC	6 cut(s)	43, 115, 502, 1472, 1549, 1607
HhaI	GCGC	2 cut(s)	339, 1905
Hin1I	GRCGYC	2 cut(s)	107, 2110
Hin6I	GCGC	2 cut(s)	337, 1903
HinP1I	GCGC	2 cut(s)	337, 1903
HincII	GTYRAC	3 cut(s)	36, 86, 2607
HindII	GTYRAC	3 cut(s)	36, 86, 2607
HpaI	GTTAAC	2 cut(s)	36, 2607
HphI	GGTGA	9 cut(s)	170, 323, 806, 959, 1160, 1287, 1709, 2927, 3103
Hpy166II	GTNNAC	3 cut(s)	36, 86, 2607
Hpy188III	TCNNGA	8 cut(s)	58, 215, 239, 416, 938, 1233, 1567, 2962
Hpy8I	GTNNAC	3 cut(s)	36, 86, 2607
Hpy99I	CGWCG	4 cut(s)	90, 109, 2115, 3080
HpyAV	CCTTC	5 cut(s)	833, 871, 1182, 1588, 1941
HpyCH4III	ACNGT	5 cut(s)	2077, 2162, 2287, 2311, 2734
HpyCH4IV	ACGT	4 cut(s)	32, 117, 2110, 2245
HpySE526I	ACGT	4 cut(s)	32, 117, 2110, 2245
Hsp92I	GRCGYC	2 cut(s)	107, 2110
HspAI	GCGC	2 cut(s)	337, 1903
KroI	GCCGGC	1 cut(s)	1110
KroNI	GCCGGC	1 cut(s)	1112
Ksp22I	TGATCA	1 cut(s)	2226
KspAI	GTTAAC	2 cut(s)	36, 2607
KspI	CCGCGG	1 cut(s)	343
MaeI	CTAG	7 cut(s)	453, 462, 1001, 2577, 2634, 2978, 3147
MaeII	ACGT	4 cut(s)	32, 117, 2110, 2245
MaeIII	GTNAC	8 cut(s)	751, 947, 1293, 1715, 1867, 1950, 2077, 2782
MbiI	CCGCTC	3 cut(s)	8, 244, 521
MfeI	CAATTG	5 cut(s)	1209, 1769, 1979, 2394, 2599
MflI	RGATCY	4 cut(s)	1192, 1266, 1357, 2958
MhlI	GDGCHC	7 cut(s)	214, 222, 285, 325, 393, 765, 3131
MlyI	GAGTC	4 cut(s)	277, 1756, 2411, 3089
MmeI	TCCRAC	3 cut(s)	1042, 1694, 2238
Mph1103I	ATGCAT	1 cut(s)	170
MroNI	GCCGGC	1 cut(s)	1110
MroXI	GAANNNNTTC	1 cut(s)	3012
MseI	TTAA	9 cut(s)	35, 675, 1121, 2027, 2313, 2352, 2558, 2606, 2715
MslI	CAYNNNNRTG	2 cut(s)	3065, 3158
MspA1I	CMGCKG	3 cut(s)	342, 805, 1786
MspR9I	CCNGG	9 cut(s)	273, 904, 922, 996, 2156, 2803, 2804, 2968, 3132
MunI	CAATTG	5 cut(s)	1209, 1769, 1979, 2394, 2599
MvaI	CCWGG	2 cut(s)	2156, 3132
MvnI	CGCG	1 cut(s)	342
NaeI	GCCGGC	1 cut(s)	1112
NciI	CCSGG	7 cut(s)	273, 904, 922, 996, 2803, 2804, 2968
NcoI	CCATGG	1 cut(s)	576
NgoMIV	GCCGGC	1 cut(s)	1110
NmeAIII	GCCGAG	1 cut(s)	233
NmuCI	GTSAC	7 cut(s)	751, 947, 1293, 1715, 1867, 2077, 2782
NsiI	ATGCAT	1 cut(s)	170
NspI	RCATGY	6 cut(s)	168, 1380, 1614, 1745, 1930, 2137
OliI	CACNNNNGTG	1 cut(s)	3065
PaeI	GCATGC	2 cut(s)	168, 1930
PaeR7I	CTCGAG	1 cut(s)	392
PaqCI	CACCTGC	1 cut(s)	1015
PciI	ACATGT	4 cut(s)	1376, 1610, 1741, 2133
PdiI	GCCGGC	1 cut(s)	1112
PdmI	GAANNNNTTC	1 cut(s)	3012
PflMI	CCANNNNNTGG	1 cut(s)	1214
PleI	GAGTC	4 cut(s)	276, 1756, 2411, 3088
PmaCI	CACGTG	1 cut(s)	118
PmlI	CACGTG	1 cut(s)	118
PpsI	GAGTC	4 cut(s)	276, 1756, 2411, 3088
Ppu21I	YACGTR	1 cut(s)	118
PscI	ACATGT	4 cut(s)	1376, 1610, 1741, 2133
PshAI	GACNNNNGTC	2 cut(s)	1342, 2754
PshBI	ATTAAT	1 cut(s)	2352
Psp124BI	GAGCTC	5 cut(s)	214, 222, 325, 393, 765
Psp1406I	AACGTT	1 cut(s)	32
Psp6I	CCWGG	2 cut(s)	2154, 3130
PspCI	CACGTG	1 cut(s)	118
PspEI	GGTNACC	1 cut(s)	1715
PspFI	CCCAGC	1 cut(s)	2117
PspGI	CCWGG	2 cut(s)	2154, 3130
PspPI	GGNCC	7 cut(s)	572, 800, 1552, 2152, 2211, 2493, 2622
PspXI	VCTCGAGB	1 cut(s)	392
PstI	CTGCAG	3 cut(s)	204, 571, 624
PstNI	CAGNNNCTG	2 cut(s)	2063, 2645
PsuI	RGATCY	4 cut(s)	1192, 1266, 1357, 2958
RsaI	GTAC	7 cut(s)	19, 654, 1393, 1528, 1697, 2018, 2799
RsaNI	GTAC	7 cut(s)	18, 653, 1392, 1527, 1696, 2017, 2798
RseI	CAYNNNNRTG	2 cut(s)	3065, 3158
SacI	GAGCTC	5 cut(s)	214, 222, 325, 393, 765
SacII	CCGCGG	1 cut(s)	343
SalI	GTCGAC	1 cut(s)	84
SaqAI	TTAA	9 cut(s)	35, 675, 1121, 2027, 2313, 2352, 2558, 2606, 2715
Sau96I	GGNCC	7 cut(s)	572, 800, 1552, 2152, 2211, 2493, 2622
SbfI	CCTGCAGG	1 cut(s)	571
SchI	GAGTC	4 cut(s)	277, 1756, 2411, 3089
ScrFI	CCNGG	9 cut(s)	273, 904, 922, 996, 2156, 2803, 2804, 2968, 3132
SdaI	CCTGCAGG	1 cut(s)	571
SduI	GDGCHC	7 cut(s)	214, 222, 285, 325, 393, 765, 3131
SfcI	CTRYAG	4 cut(s)	200, 567, 620, 1196
Sfr274I	CTCGAG	1 cut(s)	392
Sfr303I	CCGCGG	1 cut(s)	343
SgrBI	CCGCGG	1 cut(s)	343
SinI	GGWCC	2 cut(s)	1552, 2211
SlaI	CTCGAG	1 cut(s)	392
SmaI	CCCGGG	1 cut(s)	2804
SmiMI	CAYNNNNRTG	2 cut(s)	3065, 3158
SmlI	CTYRAG	4 cut(s)	324, 392, 2342, 3038
SmoI	CTYRAG	4 cut(s)	324, 392, 2342, 3038
SphI	GCATGC	2 cut(s)	168, 1930
Sse8387I	CCTGCAGG	1 cut(s)	571
SspMI	CTAG	7 cut(s)	453, 462, 1001, 2577, 2634, 2978, 3147
SstI	GAGCTC	5 cut(s)	214, 222, 325, 393, 765
StyD4I	CCNGG	9 cut(s)	271, 902, 920, 994, 2154, 2801, 2802, 2966, 3130
StyI	CCWWGG	4 cut(s)	576, 1431, 2633, 3049
TaaI	ACNGT	5 cut(s)	2077, 2162, 2287, 2311, 2734
TaiI	ACGT	4 cut(s)	35, 120, 2113, 2248
TaqI	TCGA	8 cut(s)	68, 85, 387, 393, 417, 1366, 2296, 2664
TaqII	GACCGA	1 cut(s)	2557
TatI	WGTACW	4 cut(s)	1391, 1526, 1695, 2016
Tru1I	TTAA	9 cut(s)	35, 675, 1121, 2027, 2313, 2352, 2558, 2606, 2715
Tru9I	TTAA	9 cut(s)	35, 675, 1121, 2027, 2313, 2352, 2558, 2606, 2715
TseFI	GTSAC	7 cut(s)	751, 947, 1293, 1715, 1867, 2077, 2782
Tsp45I	GTSAC	7 cut(s)	751, 947, 1293, 1715, 1867, 2077, 2782
TspGWI	ACGGA	4 cut(s)	103, 1565, 1982, 2264
TspMI	CCCGGG	1 cut(s)	2802
Van91I	CCANNNNNTGG	1 cut(s)	1214
VpaK11BI	GGWCC	2 cut(s)	1552, 2211
VspI	ATTAAT	1 cut(s)	2352
XagI	CCTNNNNNAGG	1 cut(s)	1150
XapI	RAATTY	3 cut(s)	598, 913, 1558
XceI	RCATGY	6 cut(s)	168, 1380, 1614, 1745, 1930, 2137
XhoI	CTCGAG	1 cut(s)	392
XmaI	CCCGGG	1 cut(s)	2802
XmaJI	CCTAGG	1 cut(s)	2633
XmiI	GTMKAC	1 cut(s)	85
XmnI	GAANNNNTTC	1 cut(s)	3012
XspI	CTAG	7 cut(s)	453, 462, 1001, 2577, 2634, 2978, 3147
ZraI	GACGTC	1 cut(s)	2111
Zsp2I	ATGCAT	1 cut(s)	170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.