Rh4BG117200

chaperone protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013028
Physical Location & Seq
Reverse (-)
19740222 .. 19743842
3621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG117200.1

Sequence Viewer

Length: 3126 bp
ATGAGAGCAGGCCTGAGCACGATCCAGCAAACCTTAACGCCGGAGGCGGCGAGCGTGTTGAACCACTCGATCGCCGAAGCGGGTCGACGGAACCACGGCCAAACGACGCCCCTTCATGTAGCGGCAACTCTTCTATCTTCCCCCACCGGCTTTCTCCGCCAAGCATGCATCAAATCTCACCCCAATTCCTCTCACCCTCTTCAGTGCCGAGCACTGGAGCTCTGCTTCAGTGTGGCGCTCGAGCGGTTGCCCACAGCCCAGAACATGAGCCCCGGGATGGAGCCTCCAATCTCCAATGCCCTCATGGCCGCCTTAAAGCGAGCTCAGGCTCACCAGCGCAGAGGCTGCCCGGAGCAGCAGCAACAGCCGCTCCTAGCTGTCAAAGTCGAACTCGAGCAGCTCATAATCTCGATTCTCGATGACCCAAGTGTTAGTCGGGTCATGCGGGAGGCCAGTTTCTCTAGTCCGGCTGTTAAAGCCACCATCGAACAATCTCTAAACTCTTCATCCGCCGCCGCGGCGAATTCTTCCCCAATTGGATTGGGTTTCCGGCCGGCGGCCGCCGGGCGGAGCATGTATTTGAATCCCAGGCTGCAGGGGACAGCTGGGAATTCGGGACAAAACCGAGCCGAGGAAGTTAAAAAAGTCGTTGATATTTTGTCTAGGAGCAAGAAGAGGAATCCGGTATTGGTCGGCGATACGGAGCCTGAGGCTGTGACGAAAGAGCTATTCAGGCGGATTCAGAGCATGGAATTGGGGCAAGGGCACCTCAAAAATGTCGAGGTCATTCATTTGGAGAAGGAGTTTTCTTCGCAAATATTGGGGAAGATGAAGGACTTGATGTCCTTGGTTGAGACCCGGATGGCGGGTTCGAATGGGGGAGGGATGATTCTTGATTTGGGCGACTTGAAATGGCTCGTGGAGCAGCCTGTTAGCTTGGGAGCGGTTCCGCCCGGCTCCGGTGGCCAACAGGTGGTTTCGGAGGCCGGGCGGGCGGCGGTAGCGGAAATGGGGAAGGTGTTGGGAAGGTTTGGAGAGGGTGGTGTTAATGGTAGTGGCCGGCTTTGGTTGATAGGGACTGCTACTTGTGAGACGTATTTGAGGTGCCAAGTCTATCACCCTTCAATGGAAACGGACTGGGATCTACAGGCAGTGCCGATTGCTGCCAGGACGCCGCATTCGGGATTGTTTCCTAGGATGGGGATGACGAACGGGATCCTTAGTAGCTCAGTTGAATCTTTGTCTCCGTTGAAAGGCTTTTCAACTGCTCAACCAAGGCTTGTGCCTGAGAACTTGGATGCTGCACGGAGACCAAGCTGTTGCCCGCAATGTACAGAAAGTTGTGAGCAAGAAGTAGCAAAATTGGTTGCGAAGGAGTCTGAGAAATCATCCTCTGAGTCAAAATTGGAAGCAGCCCAACCAGCACTACCACAGTGGTTGCAGAATGCTAAAGCCCAGGACAGTAATGTCAAAACATCAGATCAGTTGCAGACTAAGAATCAAGATCAGACTTTGAACCAGAAGACTCAAGAATTACGAAAGGAATGGAAAGATACGTGTACGCGCCTTCATCCTAATTTTCATCAGCATAGTTTCAGCTCAGAGAGAATTGCTCCCACACCTCTCTCAATCACCAGCATGTACAATATGAACTTGCTTGGTCGCCAACCTTTCCAACCGAAATTGCAACCAAACAAAAGCTTTGGGGCTCTGCAATTGAATACAAATCTAAAGACCAGCCAACCATCTGAACGTGCAGCAGTTTCACATCCACGAAGCCCTGTAAGGACAGACCTGGTTCTTGGGCAAAAAGAAGTCACTGAAACCATCACCCCTGAGCAAATGCATAAGGAGCGCGTCAAAGACTTTGTGGGCTGCATGCCTTCCGAACCACAGAACAAGATACTTGAAAGGCAAACAGATGAAAAGCAATTGTGCCAATTAGATGCTGATTCATTCAAAAAGCTGTACAAGGGTTTGATTGAGGTCTGGTGGCAGCCGGAAGCAGCAAACACTGTTGCTGCTACAATAACCAATTGTAAGTTGGGTAATGGAAAGAGGCGTGGTGCCGGAGCAAGGGGAGACATGTGGTTGTTGTTCATGGGTCCTGACACTGTTGGCAAGAAGAAGATGACATCAGCTCTTTCAGAAATGGTATCTGGGTCCACCCCGGTGATGATCTCCCTTAACAATAAACGCGGTAGTTGGGAATCAGACATGAGTTTTCGTGGAAAAACGGTGGTGGACCGAATAGCAGAGGCAGTTAGGAGGAACCCCTTTTCTGTAATCATGCTTGAGGACATTAATGAAGCAGATACGATAGTTCGCGGTAGCATAAAACGGGCAATGGAGAGAGGTCGTCTTGGCGACTCTTATGGCCGTGAAATCAGTCTTGGAAATGTTATCTTTATCCTCACTGCAAATTGGTTGCCAGAAAATTTGAAACACTTATCAAGCGGCAATTCTCTTGAAGAAAAGCTTGCTTGTATAGCTAGGAGTGGTTGGCAGTTAAAGCTATCCGTTTGTGGAAGAAGTGCAAAACGGCGAGCAACTTGGTTACAGAGTGTTGAAGACAGAGCCACAAAGCCTAGGAAAGATGCGGGTTCTGGACTAGGATTCGATCTGAATGAGGCTGCTGATGTTGAGGATGACAGAACAGATGGCTCACTTAATTCAAGTGATCTAACAGTTGATCATGAAGATGACCGCCTCAATAACAGGTCATTGCTCACAAGTACACCTTCATCAGTACTGAGGGAATTGCTTGATTCTGTTGACGATGCCATTGTGTTCAAGCCAGTGGATTTCAATCCCATTCGGAATAACATCACAAACTCAATCACAAGAAGATTTTCTATGATCATTGGCGATGGAGTTTCCTTTGAATTGCAAGAGGATACTGTTGAAAAGATCCTTAGTGGGATATGGCTAGGCAAGACTGGCTTAGAAGAATGGACTGAAAAGGTGCTGGTTCCGAGCCTCCAACAGTTGAAGTCCCGCCTAGGTGTGACACTTGACGAGTCAATGGTGGTTAGACTCGAAGATGATGGAGATTCGGGTTGCCGGAGACATGGAGATTGGCTGCCGAGTAGCATCAATGTGGTGGCCGACGGATTAAGACAATGA

Protein Analysis

1041

Amino Acids

114.1

Weight (kDa)

8.31

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 113 1.1e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 209 - 390 2.8e-29 SMAX1 nucleotide binding domain
AAA_2 PF07724 698 - 815 2.2e-09 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 935 - 1018 8.8e-26 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57710
fragaria_vesca FvH4_4g09820
malus_domestica MD13G1261900.v1.1 MD16G1264200.v1.1
prunus_persica Prupe.1G095200_v2.0.a1 Prupe.1G095200_v2.0.a1
pyrus_communis pycom13g24720 pycom16g23290
rosa_chinensis RchiOBHm_Chr4g0404601
rosa_laevigata RLG00000008920
rosa_multiflora Rmu_sc0002699.1_g000006
rosa_roxburghii Rroxscaffold_5G00348750
rosa_rugosa Rorug04G0047700
rosa_samantha Rh4AG123800 Rh4BG117200 Rh4CG131900 Rh4DG116700
rosa_wichuraiana Rw4G010030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1466
AccB1I GGYRCC 3 cut(s) 765, 1104, 2066
AccB7I CCANNNNNTGG 1 cut(s) 973
AccBSI CCGCTC 3 cut(s) 244, 370, 944
AccI GTMKAC 1 cut(s) 85
AccII CGCG 5 cut(s) 518, 1564, 1857, 2199, 2328
AclWI GGATC 5 cut(s) 16, 1149, 1210, 1223, 2905
AcoI YGGCCR 8 cut(s) 97, 306, 551, 558, 964, 1057, 2377, 3105
AcsI RAATTY 3 cut(s) 523, 610, 2437
AcuI CTGAAG 2 cut(s) 185, 211
AcyI GRCGYC 2 cut(s) 107, 1172
AfaI GTAC 6 cut(s) 1333, 1561, 1643, 1970, 2737, 2751
AflIII ACRYGT 2 cut(s) 1556, 2085
AhdI GACNNNNNGTC 1 cut(s) 841
AjnI CCWGG 4 cut(s) 587, 1166, 1455, 1794
AjuI GAANNNNNNNTTGG 4 cut(s) 671, 703, 2376, 2408
AleI CACNNNNGTG 1 cut(s) 2171
AloI GAACNNNNNNTCC 2 cut(s) 853, 885
Alw21I GWGCWC 4 cut(s) 20, 214, 222, 325
Alw26I GTCTC 6 cut(s) 848, 1085, 1248, 1303, 2076, 3061
AlwI GGATC 5 cut(s) 16, 1149, 1210, 1223, 2905
AlwNI CAGNNNCTG 1 cut(s) 345
Ama87I CYCGRG 3 cut(s) 239, 272, 392
ApoI RAATTY 3 cut(s) 523, 610, 2437
ArsI GACNNNNNNTTYG 2 cut(s) 1162, 1194
AseI ATTAAT 1 cut(s) 2304
Asp700I GAANNNNTTC 1 cut(s) 2851
AspA2I CCTAGG 3 cut(s) 1193, 2588, 3001
AspLEI GCGC 4 cut(s) 238, 339, 1566, 1857
AspS9I GGNCC 3 cut(s) 2105, 2163, 2245
AsuC2I CCSGG 8 cut(s) 273, 274, 350, 565, 859, 954, 988, 2171
AsuHPI GGTGA 7 cut(s) 170, 185, 323, 1109, 1624, 1822, 2185
AsuII TTCGAA 1 cut(s) 872
AvaI CYCGRG 3 cut(s) 239, 272, 392
AvaII GGWCC 3 cut(s) 2105, 2163, 2245
AvrII CCTAGG 3 cut(s) 1193, 2588, 3001
AxyI CCTNAGG 1 cut(s) 708
BaeGI GKGCMC 1 cut(s) 768
BaeI ACNNNNGTAYC 2 cut(s) 2307, 2340
BalI TGGCCA 1 cut(s) 966
BamHI GGATCC 1 cut(s) 1215
BanI GGYRCC 3 cut(s) 765, 1104, 2066
BanII GRGCYC 4 cut(s) 222, 272, 325, 1711
BauI CACGAG 1 cut(s) 917
BbsI GAAGAC 2 cut(s) 1529, 2577
Bbv12I GWGCWC 4 cut(s) 20, 214, 222, 325
BccI CCATC 9 cut(s) 271, 491, 856, 1192, 1753, 1835, 2654, 2864, 3041
BceAI ACGGC 3 cut(s) 112, 2364, 2558
BciT130I CCWGG 4 cut(s) 589, 1168, 1457, 1796
BciVI GTATCC 1 cut(s) 2890
BclI TGATCA 2 cut(s) 2692, 2859
BcnI CCSGG 8 cut(s) 273, 274, 350, 565, 859, 954, 988, 2171
BcoDI GTCTC 6 cut(s) 848, 1085, 1248, 1303, 2076, 3061
BfaI CTAG 9 cut(s) 374, 462, 663, 1194, 2493, 2589, 2612, 2930, 3002
BfmI CTRYAG 2 cut(s) 593, 1145
BfoI RGCGCY 1 cut(s) 239
BfuI GTATCC 1 cut(s) 2890
BglI GCCNNNNNGGC 3 cut(s) 305, 518, 992
BlnI CCTAGG 3 cut(s) 1193, 2588, 3001
BmcAI AGTACT 1 cut(s) 2751
Bme18I GGWCC 3 cut(s) 2105, 2163, 2245
BmeRI GACNNNNNGTC 1 cut(s) 841
BmeT110I CYCGRG 3 cut(s) 239, 272, 392
BmgT120I GGNCC 3 cut(s) 2105, 2163, 2245
BmrI ACTGGG 1 cut(s) 1147
BmsI GCATC 6 cut(s) 177, 1288, 1936, 2587, 2770, 3102
BmuI ACTGGG 1 cut(s) 1147
BpiI GAAGAC 2 cut(s) 1529, 2577
BplI GAGNNNNNCTC 2 cut(s) 1597, 1629
BpmI CTGGAG 1 cut(s) 236
Bpu10I CCTNAGC 3 cut(s) 14, 324, 1836
Bpu14I TTCGAA 1 cut(s) 872
BpuEI CTTGAG 2 cut(s) 1512, 2315
BpuMI CCSGG 8 cut(s) 273, 274, 350, 565, 859, 954, 988, 2171
BsaAI YACGTR 1 cut(s) 1557
BsaBI GATNNNNATC 1 cut(s) 2808
BsaHI GRCGYC 2 cut(s) 107, 1172
BsaI GGTCTC 2 cut(s) 848, 1303
BsaWI WCCGGW 2 cut(s) 682, 959
BsaXI ACNNNNNCTCC 4 cut(s) 354, 384, 788, 818
Bse118I RCCGGY 3 cut(s) 146, 553, 1059
Bse1I ACTGG 5 cut(s) 219, 453, 1142, 2798, 2944
Bse21I CCTNAGG 1 cut(s) 708
Bse3DI GCAATG 3 cut(s) 1334, 2352, 2723
Bse8I GATNNNNATC 1 cut(s) 2808
BseBI CCWGG 4 cut(s) 589, 1168, 1457, 1796
BseJI GATNNNNATC 1 cut(s) 2808
BseMI GCAATG 3 cut(s) 1334, 2352, 2723
BseNI ACTGG 5 cut(s) 219, 453, 1142, 2798, 2944
BseSI GKGCMC 1 cut(s) 768
BseX3I CGGCCG 2 cut(s) 551, 558
BseYI CCCAGC 1 cut(s) 605
BsgI GTGCAG 2 cut(s) 1287, 1776
Bsh1236I CGCG 5 cut(s) 518, 1564, 1857, 2199, 2328
Bsh1285I CGRYCG 3 cut(s) 72, 554, 561
BshNI GGYRCC 3 cut(s) 765, 1104, 2066
BsiEI CGRYCG 3 cut(s) 72, 554, 561
BsiHKAI GWGCWC 4 cut(s) 20, 214, 222, 325
BsiHKCI CYCGRG 3 cut(s) 239, 272, 392
BslFI GGGAC 4 cut(s) 613, 630, 1090, 2980
BsmAI GTCTC 6 cut(s) 848, 1085, 1248, 1303, 2076, 3061
BsmBI CGTCTC 1 cut(s) 1085
BsmFI GGGAC 4 cut(s) 613, 630, 1090, 2980
BsmI GAATGC 2 cut(s) 1177, 1450
Bso31I GGTCTC 2 cut(s) 848, 1303
BsoBI CYCGRG 3 cut(s) 239, 272, 392
Bsp119I TTCGAA 1 cut(s) 872
Bsp1286I GDGCHC 7 cut(s) 20, 214, 222, 272, 325, 768, 1711
Bsp1407I TGTACA 3 cut(s) 1331, 1641, 1968
BspFNI CGCG 5 cut(s) 518, 1564, 1857, 2199, 2328
BspHI TCATGA 1 cut(s) 2695
BspMAI CTGCAG 1 cut(s) 597
BspPI GGATC 5 cut(s) 16, 1149, 1210, 1223, 2905
BspT104I TTCGAA 1 cut(s) 872
BspT107I GGYRCC 3 cut(s) 765, 1104, 2066
BspTNI GGTCTC 2 cut(s) 848, 1303
BsrBI CCGCTC 3 cut(s) 244, 370, 944
BsrDI GCAATG 3 cut(s) 1334, 2352, 2723
BsrFI RCCGGY 3 cut(s) 146, 553, 1059
BsrGI TGTACA 3 cut(s) 1331, 1641, 1968
BsrI ACTGG 5 cut(s) 219, 453, 1142, 2798, 2944
BssAI RCCGGY 3 cut(s) 146, 553, 1059
BssNI GRCGYC 2 cut(s) 107, 1172
BssSI CACGAG 1 cut(s) 917
BssT1I CCWWGG 5 cut(s) 846, 1193, 1274, 2588, 3001
Bst2BI CACGAG 1 cut(s) 917
Bst2UI CCWGG 4 cut(s) 589, 1168, 1457, 1796
Bst4CI ACNGT 8 cut(s) 1434, 1463, 2017, 2116, 2239, 2689, 2902, 2988
Bst6I CTCTTC 4 cut(s) 135, 204, 508, 668
BstACI GRCGYC 2 cut(s) 107, 1172
BstAPI GCANNNNNTGC 1 cut(s) 345
BstAUI TGTACA 3 cut(s) 1331, 1641, 1968
BstBAI YACGTR 1 cut(s) 1557
BstBI TTCGAA 1 cut(s) 872
BstDSI CCRYGG 2 cut(s) 94, 516
BstFNI CGCG 5 cut(s) 518, 1564, 1857, 2199, 2328
BstH2I RGCGCY 1 cut(s) 239
BstHHI GCGC 4 cut(s) 238, 339, 1566, 1857
BstMAI GTCTC 6 cut(s) 848, 1085, 1248, 1303, 2076, 3061
BstMCI CGRYCG 3 cut(s) 72, 554, 561
BstNI CCWGG 4 cut(s) 589, 1168, 1457, 1796
BstNSI RCATGY 5 cut(s) 168, 577, 1642, 1882, 2089
BstSFI CTRYAG 2 cut(s) 593, 1145
BstSLI GKGCMC 1 cut(s) 768
BstUI CGCG 5 cut(s) 518, 1564, 1857, 2199, 2328
BstV2I GAAGAC 2 cut(s) 1529, 2577
BstX2I RGATCY 3 cut(s) 1141, 1215, 2910
BstYI RGATCY 3 cut(s) 1141, 1215, 2910
BstZI CGGCCG 2 cut(s) 551, 558
Bsu36I CCTNAGG 1 cut(s) 708
BsuI GTATCC 1 cut(s) 2890
BtgI CCRYGG 2 cut(s) 94, 516
BtgZI GCGATG 1 cut(s) 2883
BtsI GCAGTG 2 cut(s) 1158, 2415
CaiI CAGNNNCTG 1 cut(s) 345
CciI TCATGA 1 cut(s) 2695
CciNI GCGGCCGC 1 cut(s) 558
CfoI GCGC 4 cut(s) 238, 339, 1566, 1857
Cfr10I RCCGGY 3 cut(s) 146, 553, 1059
Cfr13I GGNCC 3 cut(s) 2105, 2163, 2245
Cfr42I CCGCGG 1 cut(s) 519
Cfr9I CCCGGG 1 cut(s) 272
CseI GACGC 3 cut(s) 115, 1180, 1846
CsiI ACCWGGT 1 cut(s) 1794
Csp6I GTAC 6 cut(s) 1332, 1560, 1642, 1969, 2736, 2750
CspCI CAANNNNNGTGG 2 cut(s) 1419, 1454
CviQI GTAC 6 cut(s) 1332, 1560, 1642, 1969, 2736, 2750
DrdI GACNNNNNNGTC 1 cut(s) 1466
DriI GACNNNNNGTC 1 cut(s) 841
DseDI GACNNNNNNGTC 1 cut(s) 1466
EaeI YGGCCR 8 cut(s) 97, 306, 551, 558, 964, 1057, 2377, 3105
EagI CGGCCG 2 cut(s) 551, 558
Eam1104I CTCTTC 4 cut(s) 135, 204, 508, 668
Eam1105I GACNNNNNGTC 1 cut(s) 841
EarI CTCTTC 4 cut(s) 135, 204, 508, 668
EciI GGCGGA 5 cut(s) 146, 499, 583, 751, 939
Ecl136II GAGCTC 2 cut(s) 220, 323
EclXI CGGCCG 2 cut(s) 551, 558
Eco130I CCWWGG 5 cut(s) 846, 1193, 1274, 2588, 3001
Eco147I AGGCCT 1 cut(s) 12
Eco24I GRGCYC 4 cut(s) 222, 272, 325, 1711
Eco31I GGTCTC 2 cut(s) 848, 1303
Eco47I GGWCC 3 cut(s) 2105, 2163, 2245
Eco52I CGGCCG 2 cut(s) 551, 558
Eco53kI GAGCTC 2 cut(s) 220, 323
Eco57I CTGAAG 2 cut(s) 185, 211
Eco81I CCTNAGG 1 cut(s) 708
Eco88I CYCGRG 3 cut(s) 239, 272, 392
EcoICRI GAGCTC 2 cut(s) 220, 323
EcoO109I RGGNCCY 1 cut(s) 2105
EcoRI GAATTC 2 cut(s) 523, 610
EcoRII CCWGG 4 cut(s) 587, 1166, 1455, 1794
EcoT14I CCWWGG 5 cut(s) 846, 1193, 1274, 2588, 3001
EcoT22I ATGCAT 2 cut(s) 170, 1848
EcoT38I GRGCYC 4 cut(s) 222, 272, 325, 1711
ErhI CCWWGG 5 cut(s) 846, 1193, 1274, 2588, 3001
Esp3I CGTCTC 1 cut(s) 1085
FalI AAGNNNNNCTT 6 cut(s) 2463, 2495, 2725, 2757, 2927, 2959
FaqI GGGAC 4 cut(s) 613, 630, 1090, 2980
FauI CCCGC 7 cut(s) 73, 438, 859, 984, 1332, 2593, 3005
FbaI TGATCA 2 cut(s) 2692, 2859
FblI GTMKAC 1 cut(s) 85
FriOI GRGCYC 4 cut(s) 222, 272, 325, 1711
FspBI CTAG 9 cut(s) 374, 462, 663, 1194, 2493, 2589, 2612, 2930, 3002
GlaI GCGC 4 cut(s) 237, 338, 1565, 1856
GsaI CCCAGC 1 cut(s) 609
GsuI CTGGAG 1 cut(s) 236
HaeII RGCGCY 1 cut(s) 239
HgaI GACGC 3 cut(s) 115, 1180, 1846
HhaI GCGC 4 cut(s) 238, 339, 1566, 1857
Hin1I GRCGYC 2 cut(s) 107, 1172
Hin6I GCGC 4 cut(s) 236, 337, 1564, 1855
HinP1I GCGC 4 cut(s) 236, 337, 1564, 1855
HincII GTYRAC 2 cut(s) 86, 2776
HindII GTYRAC 2 cut(s) 86, 2776
HindIII AAGCTT 2 cut(s) 1699, 2477
HphI GGTGA 7 cut(s) 170, 185, 323, 1109, 1624, 1822, 2185
Hpy166II GTNNAC 6 cut(s) 86, 1560, 2166, 2245, 2738, 2776
Hpy8I GTNNAC 6 cut(s) 86, 1560, 2166, 2245, 2738, 2776
Hpy99I CGWCG 3 cut(s) 90, 109, 3113
HpyCH4III ACNGT 8 cut(s) 1434, 1463, 2017, 2116, 2239, 2689, 2902, 2988
HpyCH4IV ACGT 3 cut(s) 1094, 1556, 1753
HpySE526I ACGT 3 cut(s) 1094, 1556, 1753
Hsp92I GRCGYC 2 cut(s) 107, 1172
HspAI GCGC 4 cut(s) 236, 337, 1564, 1855
KroI GCCGGC 2 cut(s) 553, 1059
KroNI GCCGGC 2 cut(s) 555, 1061
Ksp22I TGATCA 2 cut(s) 2692, 2859
KspI CCGCGG 1 cut(s) 519
LweI GCATC 6 cut(s) 177, 1288, 1936, 2587, 2770, 3102
MabI ACCWGGT 1 cut(s) 1794
MaeI CTAG 9 cut(s) 374, 462, 663, 1194, 2493, 2589, 2612, 2930, 3002
MaeII ACGT 3 cut(s) 1094, 1556, 1753
MaeIII GTNAC 4 cut(s) 715, 1816, 2556, 3007
MbiI CCGCTC 3 cut(s) 244, 370, 944
MfeI CAATTG 4 cut(s) 534, 1715, 1931, 2035
MflI RGATCY 3 cut(s) 1141, 1215, 2910
MhlI GDGCHC 7 cut(s) 20, 214, 222, 272, 325, 768, 1711
MlsI TGGCCA 1 cut(s) 966
MluNI TGGCCA 1 cut(s) 966
MlyI GAGTC 6 cut(s) 1385, 1406, 1519, 2363, 3029, 3030
MmeI TCCRAC 2 cut(s) 1699, 3007
Mox20I TGGCCA 1 cut(s) 966
Mph1103I ATGCAT 2 cut(s) 170, 1848
MroNI GCCGGC 2 cut(s) 553, 1059
MroXI GAANNNNTTC 1 cut(s) 2851
MscI TGGCCA 1 cut(s) 966
MslI CAYNNNNRTG 4 cut(s) 1637, 2171, 2700, 3098
Msp20I TGGCCA 1 cut(s) 966
MspA1I CMGCKG 2 cut(s) 518, 605
MunI CAATTG 4 cut(s) 534, 1715, 1931, 2035
Mva1269I GAATGC 2 cut(s) 1177, 1450
MvaI CCWGG 4 cut(s) 589, 1168, 1457, 1796
MvnI CGCG 5 cut(s) 518, 1564, 1857, 2199, 2328
NaeI GCCGGC 2 cut(s) 555, 1061
NciI CCSGG 8 cut(s) 273, 274, 350, 565, 859, 954, 988, 2171
NgoMIV GCCGGC 2 cut(s) 553, 1059
NmeAIII GCCGAG 3 cut(s) 233, 655, 3111
NmuCI GTSAC 3 cut(s) 715, 1816, 3007
NotI GCGGCCGC 1 cut(s) 558
NsiI ATGCAT 2 cut(s) 170, 1848
NspI RCATGY 5 cut(s) 168, 577, 1642, 1882, 2089
NspV TTCGAA 1 cut(s) 872
OliI CACNNNNGTG 1 cut(s) 2171
PaeI GCATGC 2 cut(s) 168, 1882
PaeR7I CTCGAG 2 cut(s) 239, 392
PagI TCATGA 1 cut(s) 2695
PceI AGGCCT 1 cut(s) 12
PciI ACATGT 1 cut(s) 2085
PctI GAATGC 2 cut(s) 1177, 1450
PdiI GCCGGC 2 cut(s) 555, 1061
PdmI GAANNNNTTC 1 cut(s) 2851
PflMI CCANNNNNTGG 1 cut(s) 973
Ple19I CGATCG 1 cut(s) 72
PleI GAGTC 6 cut(s) 1384, 1405, 1519, 2363, 3028, 3030
PpsI GAGTC 6 cut(s) 1384, 1405, 1519, 2363, 3028, 3030
Ppu21I YACGTR 1 cut(s) 1557
PpuMI RGGWCCY 1 cut(s) 2105
PscI ACATGT 1 cut(s) 2085
PshBI ATTAAT 1 cut(s) 2304
Psp124BI GAGCTC 2 cut(s) 222, 325
Psp5II RGGWCCY 1 cut(s) 2105
Psp6I CCWGG 4 cut(s) 587, 1166, 1455, 1794
PspFI CCCAGC 1 cut(s) 605
PspGI CCWGG 4 cut(s) 587, 1166, 1455, 1794
PspPI GGNCC 3 cut(s) 2105, 2163, 2245
PspPPI RGGWCCY 1 cut(s) 2105
PspXI VCTCGAGB 2 cut(s) 239, 392
PstI CTGCAG 1 cut(s) 597
PstNI CAGNNNCTG 1 cut(s) 345
PsuI RGATCY 3 cut(s) 1141, 1215, 2910
PvuI CGATCG 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 6 cut(s) 1333, 1561, 1643, 1970, 2737, 2751
RsaNI GTAC 6 cut(s) 1332, 1560, 1642, 1969, 2736, 2750
RseI CAYNNNNRTG 4 cut(s) 1637, 2171, 2700, 3098
SacI GAGCTC 2 cut(s) 222, 325
SacII CCGCGG 1 cut(s) 519
SalI GTCGAC 1 cut(s) 84
Sau96I GGNCC 3 cut(s) 2105, 2163, 2245
ScaI AGTACT 1 cut(s) 2751
SchI GAGTC 6 cut(s) 1385, 1406, 1519, 2363, 3029, 3030
SduI GDGCHC 7 cut(s) 20, 214, 222, 272, 325, 768, 1711
SexAI ACCWGGT 1 cut(s) 1794
SfaNI GCATC 6 cut(s) 177, 1288, 1936, 2587, 2770, 3102
SfcI CTRYAG 2 cut(s) 593, 1145
Sfr274I CTCGAG 2 cut(s) 239, 392
Sfr303I CCGCGG 1 cut(s) 519
SfuI TTCGAA 1 cut(s) 872
SgrBI CCGCGG 1 cut(s) 519
SinI GGWCC 3 cut(s) 2105, 2163, 2245
SlaI CTCGAG 2 cut(s) 239, 392
SmaI CCCGGG 1 cut(s) 274
SmiMI CAYNNNNRTG 4 cut(s) 1637, 2171, 2700, 3098
SmlI CTYRAG 4 cut(s) 239, 392, 1527, 2294
SmoI CTYRAG 4 cut(s) 239, 392, 1527, 2294
SphI GCATGC 2 cut(s) 168, 1882
SseBI AGGCCT 1 cut(s) 12
SspI AATATT 1 cut(s) 819
SspMI CTAG 9 cut(s) 374, 462, 663, 1194, 2493, 2589, 2612, 2930, 3002
SstI GAGCTC 2 cut(s) 222, 325
StuI AGGCCT 1 cut(s) 12
StyI CCWWGG 5 cut(s) 846, 1193, 1274, 2588, 3001
TaaI ACNGT 8 cut(s) 1434, 1463, 2017, 2116, 2239, 2689, 2902, 2988
TaiI ACGT 3 cut(s) 1097, 1559, 1756
TaqII GACCGA 1 cut(s) 2262
TatI WGTACW 5 cut(s) 1331, 1641, 1968, 2735, 2749
TseFI GTSAC 3 cut(s) 715, 1816, 3007
Tsp45I GTSAC 3 cut(s) 715, 1816, 3007
TspGWI ACGGA 7 cut(s) 103, 716, 1148, 1236, 1321, 2509, 3126
TspMI CCCGGG 1 cut(s) 272
Van91I CCANNNNNTGG 1 cut(s) 973
VpaK11BI GGWCC 3 cut(s) 2105, 2163, 2245
VspI ATTAAT 1 cut(s) 2304
XapI RAATTY 3 cut(s) 523, 610, 2437
XceI RCATGY 5 cut(s) 168, 577, 1642, 1882, 2089
XcmI CCANNNNNNNNNTGG 2 cut(s) 301, 2041
XhoI CTCGAG 2 cut(s) 239, 392
XmaI CCCGGG 1 cut(s) 272
XmaJI CCTAGG 3 cut(s) 1193, 2588, 3001
XmiI GTMKAC 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 2851
XspI CTAG 9 cut(s) 374, 462, 663, 1194, 2493, 2589, 2612, 2930, 3002
ZrmI AGTACT 1 cut(s) 2751
Zsp2I ATGCAT 2 cut(s) 170, 1848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.