Rh4CG131900

chaperone protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0013028
Physical Location & Seq
Reverse (-)
26027145 .. 26030805
3661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG131900.1

Sequence Viewer

Length: 3156 bp
ATGAGAGCAGGCCTGAGCACGATCCAGCAAACCTTAACGCCGGAGGCGGCGAGCGTCTTGAACCACTCGATCGCCGAAGCGGGTCGTCGGAACCACGGCCAAACGACGCCCCTTCATGTAGCGGCAACTCTTCTATCTTCCCCCACCGGCTTTCTCCGCCAAGCATGCATCAAATCTCACCCCAATTCCTCTCACCCTCTTCAGTGCCGAGCACTGGAGCTCTGCTTCAGTGTGGCGCTGGAGCGGTTGCCCACCGCCCAGAACATGAGCCCCGGGATGGAGCCTCCAATCTCCAATGCCCTCATGGCCGCCTTAAAGCGAGCTCAGGCTCACCAGCGCAGAGGCTGCCCGGAGCAGCAGCAACAGCCGCTCCTAGCTGTCAAAGTCGAACTCGAGCAGCTCATAATCTCGATTCTCGATGACCCAAGTGTTAGTCGGGTCATGCGGGAGGCCAGTTTCTCTAGTCCGGCTGTTAAAGCCACCATTGAACAATCTCTAAACTCTTCATCCGCCGCCGCGGCAGCGGCCTCCACCGCCGCCGCGGGAAATTCTTCCCCAATTGGATTGGGGTTCCGGCCGGCGGCCCCGGCCGCCGGGCGGAGCATGTATTTGAATCCCAGGCTGCAGGGGACAGCTGGGAATTCGGGACAAAACCGAGCCGAGGAAGTTAAAAAAGTCGTTGATATTTTGTCTAGGAGCAAGAAGAGGAATCCGGTATTGGTCGGCGATACGGAGCCCGACGCTGTGACGAAAGAGCTATTCAGGCGGATTCAGAGCATGGAATTGGGGCAAGGGCACCTCAAAAATGTCGAGGTCATTCATTTGGAGAAGGAGTTTTCTTCGCAAATATTGGGGAAGATGAAGGACTTGATGTCCTTGGTTGAGACCCGGATGGCGGGTTCGAATGGGGGAGGGATGATTCTTGATTTGGGCGACTTAAAATGGCTCGTGGAGCAGCCTGTTAGCTTGGGAGCGGTTCCGCCCGGCTCCGGTGGCCAACAGGTGGTTTCGGAGGCCGGGCGGGCGGCGGTGGCGGAAATGGGGAAGGTGTTGGGAAGGTTTGGAGAGGGTGGTGTTAATGGTAGTGGCCGGCTTTGGTTGATAGGGACTGCTACTTGTGAGACGTATTTGAGGTGCCAAGTCTATCACCCTTCCATGGAAACGGACTGGGATCTACAGGCAGTGCCGATTGCTGCCAGGACGCCGCATTCGGGATTGTTTCCTAGGATGGGGATGACAAACGGGATCCTTAGTAGCTCAGTTGAATCTTTGTCTCCGTTGAAAGGCTTTTCAACTGCTCAACCAAGGCTTGTGCCTGAGAACTTGGATGCTGCACGGAGAACAAGCTGTTGCCCGCAATGTACAGAAAGTTGTGAGCAAGAAGTAGCAAAATTGGTTGCGAAGGAGTCTGAGAAATCATCCTCGGAGTCAAAATCGGAAGCAGCTCAACCAGCACTACCACAGTGGTTGCAGAATGCTAAAGACCAGGACAGTAATGTCAAAACATCAGATCAGTTGCAGACTAAGAATCAAGATCAGACTTTGAACCAGAAGACTGAAGAATTACGAAAGGAATGGAAAGATACGTGTAAGCGCCTTCATCCTAATTTTCATCAGCATAGTTTCAGCTCAGAGAGAATTGCTCCCACACCTCTCTCAATCACCAGCATGTATAATATGAACTTGCTTGGTCGCCAACCTTTCCAACCAAAATTGCAACCAAACAAAAGCTTTGGGGCTCTGCAATTGAACACAAATCTAAAGACCAGCCAACCATCTGAACGTGCAGCAGTTTCACATCCACGAAGCCCTGTAAGGACGGACCTGGTTCTTGGGCAAAAAGAAGTCACTGAAACCTTCACCCCTGAGCAAATGCATAAGGAGCGCGTCAAAGACTTTATGGGCTGCATGCCTTCTGAACCACAGAACAAGATACTTGAAAGGCAAACAGATGAAAAGCAATTGTGCCAATTAGATGCTGATTCATTCAAAAAGCTGTACAAGGGTTTGATTGAGGTCTGGTGGCAGCCGGAAGCAGCAAACGCTGTTGCTGCTACGATAAACAATTGTAAGTTGGGTAATGGAAAGAGGCGTGGTGCCGGAGCAAGGGGAGACATGTGGTTGTTGTTCATGGGTCCTGACACTGTTGGCAAGAAGAAGATGACATCAGCTCTTTCAGAAATGGTATCTGGGTCCACCCCGGTGATGATCTCCCTTAACAATAAACGCGGTAGTTGGGAATCAGACATGAGTTTTCGGGGAAAAACGGTGGTGGACCGAATAGCAGAGGCAGTTAGGAGGAACCCCTTTTCTGTAATCATGCTTGAGGACATTAATGAAGCAGATACGATAGTTCGCGGTAGCATAAAACGGGCAATGGAGAGAGGTCGTCTTGGCGACTCTTATGGCCGTGAAATCAGTCTTGGAAATGTTATCTTTATCCTCACTGCAAATTGGTTGCCAGAAAATTTGAAACACTTATCAAGCGGCAATTCTCTTGAAGAAAAGCTTGCTTGTATAGCTAGGAGTGGTTGGCAGTTAAAGCTATCCGTTTGTGGAAGAAGTGCAAAACGGCGAGCAACTTGGTTACAGAGTGTTGAAGACAGAGCCACAAAGCCTAGGAAAGATGCGGGTTCTGGACTAGGATTCGATCTGAATGAGACTGCTGATGTTGAGGATGACAGAACAGATGGCTCACTTAATTCAAGTGATCTAACAGTTGATCATGAAGATGACCGCCTCAATAACAGGTCATTGCTCACAAGTACACCTTCATCAGTACTGAGGGAATTGCTTGATTCTGTTGACGATGCCATTGTGTTCAAGCCAGTGGATTTCAATCCCATTCGGAATAACATCACAAACTCAATCACAAGAAGATTTTCAATGATCATTGGCGATGGAGTTTCCTTTGAATTGCAAGCGGATACTGTTGAAAAGATCCTTAGTGGGATATGGCTAGGCAAGACTGGCTTAGAAGAATGGACTGAAAAGGTGCTGGTTCCGAGCCTTCAACAGTTGAAGTCCCGCCTAGGTGTGACACTTGACGAGTCAATGGTGGTTAGACTCGAAGATGATGGAGATTCGGGTTGCCGGAGACATGGAGATTGGCTGCCGGGCAGCATCAATGTGGTGGCCGACGGATTAAGACAATGA

Protein Analysis

1051

Amino Acids

114.9

Weight (kDa)

8.31

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 113 1.1e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 219 - 400 8.9e-29 SMAX1 nucleotide binding domain
AAA_2 PF07724 708 - 825 2.2e-09 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 945 - 1028 2.8e-25 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57710
fragaria_vesca FvH4_4g09820
malus_domestica MD13G1261900.v1.1 MD16G1264200.v1.1
prunus_persica Prupe.1G095200_v2.0.a1 Prupe.1G095200_v2.0.a1
pyrus_communis pycom13g24720 pycom16g23290
rosa_chinensis RchiOBHm_Chr4g0404601
rosa_laevigata RLG00000008920
rosa_multiflora Rmu_sc0002699.1_g000006
rosa_roxburghii Rroxscaffold_5G00348750
rosa_rugosa Rorug04G0047700
rosa_samantha Rh4AG123800 Rh4BG117200 Rh4CG131900 Rh4DG116700
rosa_wichuraiana Rw4G010030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1496
AccB1I GGYRCC 3 cut(s) 795, 1134, 2096
AccB7I CCANNNNNTGG 1 cut(s) 1003
AccBSI CCGCTC 3 cut(s) 244, 370, 974
AccII CGCG 5 cut(s) 518, 542, 1887, 2229, 2358
AclWI GGATC 5 cut(s) 16, 1179, 1240, 1253, 2935
AcoI YGGCCR 8 cut(s) 97, 306, 575, 588, 994, 1087, 2407, 3135
AcsI RAATTY 3 cut(s) 547, 640, 2467
AcuI CTGAAG 3 cut(s) 185, 211, 1578
AcyI GRCGYC 2 cut(s) 107, 1202
AfaI GTAC 4 cut(s) 1363, 2000, 2767, 2781
AflIII ACRYGT 2 cut(s) 1586, 2115
AhdI GACNNNNNGTC 1 cut(s) 871
AjnI CCWGG 4 cut(s) 617, 1196, 1485, 1824
AjuI GAANNNNNNNTTGG 4 cut(s) 701, 733, 2406, 2438
AleI CACNNNNGTG 1 cut(s) 2201
AloI GAACNNNNNNTCC 2 cut(s) 883, 915
Alw21I GWGCWC 4 cut(s) 20, 214, 222, 325
Alw26I GTCTC 6 cut(s) 878, 1115, 1278, 2106, 2654, 3091
AlwI GGATC 5 cut(s) 16, 1179, 1240, 1253, 2935
AlwNI CAGNNNCTG 1 cut(s) 345
Ama87I CYCGRG 2 cut(s) 272, 392
ApoI RAATTY 3 cut(s) 547, 640, 2467
ArsI GACNNNNNNTTYG 2 cut(s) 1192, 1224
AseI ATTAAT 1 cut(s) 2334
Asp700I GAANNNNTTC 2 cut(s) 550, 2881
AspA2I CCTAGG 3 cut(s) 1223, 2618, 3031
AspLEI GCGC 4 cut(s) 238, 339, 1596, 1887
AspS9I GGNCC 5 cut(s) 583, 1822, 2135, 2193, 2275
AsuHPI GGTGA 7 cut(s) 170, 185, 323, 1139, 1654, 1852, 2215
AsuII TTCGAA 1 cut(s) 902
AvaI CYCGRG 2 cut(s) 272, 392
AvaII GGWCC 4 cut(s) 1822, 2135, 2193, 2275
AvrII CCTAGG 3 cut(s) 1223, 2618, 3031
BaeGI GKGCMC 1 cut(s) 798
BaeI ACNNNNGTAYC 2 cut(s) 2337, 2370
BalI TGGCCA 1 cut(s) 996
BamHI GGATCC 1 cut(s) 1245
BanI GGYRCC 3 cut(s) 795, 1134, 2096
BanII GRGCYC 5 cut(s) 222, 272, 325, 738, 1741
BauI CACGAG 1 cut(s) 947
BbsI GAAGAC 2 cut(s) 1559, 2607
Bbv12I GWGCWC 4 cut(s) 20, 214, 222, 325
BccI CCATC 7 cut(s) 271, 886, 1222, 1783, 2684, 2894, 3071
BceAI ACGGC 3 cut(s) 112, 2394, 2588
BciT130I CCWGG 4 cut(s) 619, 1198, 1487, 1826
BciVI GTATCC 1 cut(s) 2920
BclI TGATCA 2 cut(s) 2722, 2889
BcoDI GTCTC 6 cut(s) 878, 1115, 1278, 2106, 2654, 3091
BfaI CTAG 9 cut(s) 374, 462, 693, 1224, 2523, 2619, 2642, 2960, 3032
BfmI CTRYAG 2 cut(s) 623, 1175
BfoI RGCGCY 2 cut(s) 239, 1597
BfuI GTATCC 1 cut(s) 2920
BglI GCCNNNNNGGC 3 cut(s) 305, 518, 1022
BlnI CCTAGG 3 cut(s) 1223, 2618, 3031
BmcAI AGTACT 1 cut(s) 2781
Bme18I GGWCC 4 cut(s) 1822, 2135, 2193, 2275
BmeRI GACNNNNNGTC 1 cut(s) 871
BmeT110I CYCGRG 2 cut(s) 272, 392
BmgT120I GGNCC 5 cut(s) 583, 1822, 2135, 2193, 2275
BmrI ACTGGG 1 cut(s) 1177
BmsI GCATC 6 cut(s) 177, 1318, 1966, 2617, 2800, 3132
BmuI ACTGGG 1 cut(s) 1177
BpiI GAAGAC 2 cut(s) 1559, 2607
BplI GAGNNNNNCTC 2 cut(s) 1627, 1659
BpmI CTGGAG 2 cut(s) 236, 260
Bpu10I CCTNAGC 3 cut(s) 14, 324, 1866
Bpu14I TTCGAA 1 cut(s) 902
BpuEI CTTGAG 1 cut(s) 2345
BsaAI YACGTR 1 cut(s) 1587
BsaBI GATNNNNATC 1 cut(s) 2838
BsaHI GRCGYC 2 cut(s) 107, 1202
BsaI GGTCTC 1 cut(s) 878
BsaWI WCCGGW 2 cut(s) 712, 989
BsaXI ACNNNNNCTCC 4 cut(s) 354, 384, 818, 848
Bse118I RCCGGY 3 cut(s) 146, 577, 1089
Bse1I ACTGG 5 cut(s) 219, 453, 1172, 2828, 2974
Bse3DI GCAATG 3 cut(s) 1364, 2382, 2753
Bse8I GATNNNNATC 1 cut(s) 2838
BseBI CCWGG 4 cut(s) 619, 1198, 1487, 1826
BseJI GATNNNNATC 1 cut(s) 2838
BseMI GCAATG 3 cut(s) 1364, 2382, 2753
BseMII CTCAG 8 cut(s) 5, 338, 1272, 1308, 1401, 1644, 1857, 2774
BseNI ACTGG 5 cut(s) 219, 453, 1172, 2828, 2974
BseSI GKGCMC 1 cut(s) 798
BseX3I CGGCCG 2 cut(s) 575, 588
BseYI CCCAGC 1 cut(s) 635
BsgI GTGCAG 2 cut(s) 1317, 1806
Bsh1236I CGCG 5 cut(s) 518, 542, 1887, 2229, 2358
Bsh1285I CGRYCG 3 cut(s) 72, 578, 591
BshNI GGYRCC 3 cut(s) 795, 1134, 2096
BsiEI CGRYCG 3 cut(s) 72, 578, 591
BsiHKAI GWGCWC 4 cut(s) 20, 214, 222, 325
BsiHKCI CYCGRG 2 cut(s) 272, 392
BslFI GGGAC 4 cut(s) 643, 660, 1120, 3010
BsmAI GTCTC 6 cut(s) 878, 1115, 1278, 2106, 2654, 3091
BsmBI CGTCTC 1 cut(s) 1115
BsmFI GGGAC 4 cut(s) 643, 660, 1120, 3010
BsmI GAATGC 2 cut(s) 1207, 1480
Bso31I GGTCTC 1 cut(s) 878
BsoBI CYCGRG 2 cut(s) 272, 392
Bsp119I TTCGAA 1 cut(s) 902
Bsp1286I GDGCHC 8 cut(s) 20, 214, 222, 272, 325, 738, 798, 1741
Bsp1407I TGTACA 2 cut(s) 1361, 1998
Bsp19I CCATGG 1 cut(s) 1155
BspCNI CTCAG 8 cut(s) 6, 337, 1271, 1309, 1402, 1643, 1858, 2775
BspFNI CGCG 5 cut(s) 518, 542, 1887, 2229, 2358
BspHI TCATGA 1 cut(s) 2725
BspMAI CTGCAG 1 cut(s) 627
BspPI GGATC 5 cut(s) 16, 1179, 1240, 1253, 2935
BspT104I TTCGAA 1 cut(s) 902
BspT107I GGYRCC 3 cut(s) 795, 1134, 2096
BspTNI GGTCTC 1 cut(s) 878
BsrBI CCGCTC 3 cut(s) 244, 370, 974
BsrDI GCAATG 3 cut(s) 1364, 2382, 2753
BsrFI RCCGGY 3 cut(s) 146, 577, 1089
BsrGI TGTACA 2 cut(s) 1361, 1998
BsrI ACTGG 5 cut(s) 219, 453, 1172, 2828, 2974
BssAI RCCGGY 3 cut(s) 146, 577, 1089
BssNI GRCGYC 2 cut(s) 107, 1202
BssSI CACGAG 1 cut(s) 947
BssT1I CCWWGG 6 cut(s) 876, 1155, 1223, 1304, 2618, 3031
Bst2BI CACGAG 1 cut(s) 947
Bst2UI CCWGG 4 cut(s) 619, 1198, 1487, 1826
Bst4CI ACNGT 7 cut(s) 1464, 1493, 2146, 2269, 2719, 2932, 3018
Bst6I CTCTTC 4 cut(s) 135, 204, 508, 698
BstACI GRCGYC 2 cut(s) 107, 1202
BstAPI GCANNNNNTGC 1 cut(s) 345
BstAUI TGTACA 2 cut(s) 1361, 1998
BstBAI YACGTR 1 cut(s) 1587
BstBI TTCGAA 1 cut(s) 902
BstDSI CCRYGG 4 cut(s) 94, 516, 540, 1155
BstFNI CGCG 5 cut(s) 518, 542, 1887, 2229, 2358
BstH2I RGCGCY 2 cut(s) 239, 1597
BstHHI GCGC 4 cut(s) 238, 339, 1596, 1887
BstMAI GTCTC 6 cut(s) 878, 1115, 1278, 2106, 2654, 3091
BstMCI CGRYCG 3 cut(s) 72, 578, 591
BstNI CCWGG 4 cut(s) 619, 1198, 1487, 1826
BstNSI RCATGY 5 cut(s) 168, 607, 1672, 1912, 2119
BstSFI CTRYAG 2 cut(s) 623, 1175
BstSLI GKGCMC 1 cut(s) 798
BstUI CGCG 5 cut(s) 518, 542, 1887, 2229, 2358
BstV2I GAAGAC 2 cut(s) 1559, 2607
BstX2I RGATCY 3 cut(s) 1171, 1245, 2940
BstYI RGATCY 3 cut(s) 1171, 1245, 2940
BstZI CGGCCG 2 cut(s) 575, 588
BsuI GTATCC 1 cut(s) 2920
BtgI CCRYGG 4 cut(s) 94, 516, 540, 1155
BtgZI GCGATG 1 cut(s) 2913
BtsI GCAGTG 2 cut(s) 1188, 2445
BtsIMutI CAGTG 9 cut(s) 209, 212, 235, 1188, 1469, 1848, 2142, 2445, 2835
CaiI CAGNNNCTG 1 cut(s) 345
CciI TCATGA 1 cut(s) 2725
CfoI GCGC 4 cut(s) 238, 339, 1596, 1887
Cfr10I RCCGGY 3 cut(s) 146, 577, 1089
Cfr13I GGNCC 5 cut(s) 583, 1822, 2135, 2193, 2275
Cfr42I CCGCGG 2 cut(s) 519, 543
Cfr9I CCCGGG 1 cut(s) 272
CseI GACGC 5 cut(s) 43, 115, 749, 1210, 1876
CsiI ACCWGGT 1 cut(s) 1824
Csp6I GTAC 4 cut(s) 1362, 1999, 2766, 2780
CspCI CAANNNNNGTGG 2 cut(s) 1449, 1484
CviQI GTAC 4 cut(s) 1362, 1999, 2766, 2780
DrdI GACNNNNNNGTC 1 cut(s) 1496
DriI GACNNNNNGTC 1 cut(s) 871
DseDI GACNNNNNNGTC 1 cut(s) 1496
EaeI YGGCCR 8 cut(s) 97, 306, 575, 588, 994, 1087, 2407, 3135
EagI CGGCCG 2 cut(s) 575, 588
Eam1104I CTCTTC 4 cut(s) 135, 204, 508, 698
Eam1105I GACNNNNNGTC 1 cut(s) 871
EarI CTCTTC 4 cut(s) 135, 204, 508, 698
EciI GGCGGA 6 cut(s) 146, 499, 613, 781, 969, 1049
Ecl136II GAGCTC 2 cut(s) 220, 323
EclXI CGGCCG 2 cut(s) 575, 588
Eco130I CCWWGG 6 cut(s) 876, 1155, 1223, 1304, 2618, 3031
Eco147I AGGCCT 1 cut(s) 12
Eco24I GRGCYC 5 cut(s) 222, 272, 325, 738, 1741
Eco31I GGTCTC 1 cut(s) 878
Eco47I GGWCC 4 cut(s) 1822, 2135, 2193, 2275
Eco52I CGGCCG 2 cut(s) 575, 588
Eco53kI GAGCTC 2 cut(s) 220, 323
Eco57I CTGAAG 3 cut(s) 185, 211, 1578
Eco88I CYCGRG 2 cut(s) 272, 392
EcoICRI GAGCTC 2 cut(s) 220, 323
EcoO109I RGGNCCY 1 cut(s) 2135
EcoRI GAATTC 1 cut(s) 640
EcoRII CCWGG 4 cut(s) 617, 1196, 1485, 1824
EcoT14I CCWWGG 6 cut(s) 876, 1155, 1223, 1304, 2618, 3031
EcoT22I ATGCAT 2 cut(s) 170, 1878
EcoT38I GRGCYC 5 cut(s) 222, 272, 325, 738, 1741
ErhI CCWWGG 6 cut(s) 876, 1155, 1223, 1304, 2618, 3031
Esp3I CGTCTC 1 cut(s) 1115
FalI AAGNNNNNCTT 6 cut(s) 2493, 2525, 2755, 2787, 2957, 2989
FaqI GGGAC 4 cut(s) 643, 660, 1120, 3010
FauI CCCGC 8 cut(s) 73, 438, 535, 889, 1014, 1362, 2623, 3035
FbaI TGATCA 2 cut(s) 2722, 2889
FriOI GRGCYC 5 cut(s) 222, 272, 325, 738, 1741
FspBI CTAG 9 cut(s) 374, 462, 693, 1224, 2523, 2619, 2642, 2960, 3032
GlaI GCGC 4 cut(s) 237, 338, 1595, 1886
GsaI CCCAGC 1 cut(s) 639
GsuI CTGGAG 2 cut(s) 236, 260
HaeII RGCGCY 2 cut(s) 239, 1597
HgaI GACGC 5 cut(s) 43, 115, 749, 1210, 1876
HhaI GCGC 4 cut(s) 238, 339, 1596, 1887
Hin1I GRCGYC 2 cut(s) 107, 1202
Hin6I GCGC 4 cut(s) 236, 337, 1594, 1885
HinP1I GCGC 4 cut(s) 236, 337, 1594, 1885
HincII GTYRAC 1 cut(s) 2806
HindII GTYRAC 1 cut(s) 2806
HindIII AAGCTT 2 cut(s) 1729, 2507
HphI GGTGA 7 cut(s) 170, 185, 323, 1139, 1654, 1852, 2215
Hpy166II GTNNAC 4 cut(s) 2196, 2275, 2768, 2806
Hpy8I GTNNAC 4 cut(s) 2196, 2275, 2768, 2806
Hpy99I CGWCG 4 cut(s) 90, 109, 743, 3143
HpyCH4III ACNGT 7 cut(s) 1464, 1493, 2146, 2269, 2719, 2932, 3018
HpyCH4IV ACGT 3 cut(s) 1124, 1586, 1783
HpySE526I ACGT 3 cut(s) 1124, 1586, 1783
Hsp92I GRCGYC 2 cut(s) 107, 1202
HspAI GCGC 4 cut(s) 236, 337, 1594, 1885
KroI GCCGGC 2 cut(s) 577, 1089
KroNI GCCGGC 2 cut(s) 579, 1091
Ksp22I TGATCA 2 cut(s) 2722, 2889
KspI CCGCGG 2 cut(s) 519, 543
LweI GCATC 6 cut(s) 177, 1318, 1966, 2617, 2800, 3132
MabI ACCWGGT 1 cut(s) 1824
MaeI CTAG 9 cut(s) 374, 462, 693, 1224, 2523, 2619, 2642, 2960, 3032
MaeII ACGT 3 cut(s) 1124, 1586, 1783
MaeIII GTNAC 4 cut(s) 745, 1846, 2586, 3037
MbiI CCGCTC 3 cut(s) 244, 370, 974
MfeI CAATTG 4 cut(s) 558, 1745, 1961, 2065
MflI RGATCY 3 cut(s) 1171, 1245, 2940
MhlI GDGCHC 8 cut(s) 20, 214, 222, 272, 325, 738, 798, 1741
MlsI TGGCCA 1 cut(s) 996
MluNI TGGCCA 1 cut(s) 996
MlyI GAGTC 5 cut(s) 1415, 1436, 2393, 3059, 3060
MmeI TCCRAC 2 cut(s) 68, 1729
Mox20I TGGCCA 1 cut(s) 996
Mph1103I ATGCAT 2 cut(s) 170, 1878
MroNI GCCGGC 2 cut(s) 577, 1089
MroXI GAANNNNTTC 2 cut(s) 550, 2881
MscI TGGCCA 1 cut(s) 996
MslI CAYNNNNRTG 4 cut(s) 1667, 2201, 2730, 3128
Msp20I TGGCCA 1 cut(s) 996
MspA1I CMGCKG 4 cut(s) 518, 524, 542, 635
MunI CAATTG 4 cut(s) 558, 1745, 1961, 2065
Mva1269I GAATGC 2 cut(s) 1207, 1480
MvaI CCWGG 4 cut(s) 619, 1198, 1487, 1826
MvnI CGCG 5 cut(s) 518, 542, 1887, 2229, 2358
NaeI GCCGGC 2 cut(s) 579, 1091
NcoI CCATGG 1 cut(s) 1155
NgoMIV GCCGGC 2 cut(s) 577, 1089
NmeAIII GCCGAG 2 cut(s) 233, 685
NmuCI GTSAC 3 cut(s) 745, 1846, 3037
NsiI ATGCAT 2 cut(s) 170, 1878
NspI RCATGY 5 cut(s) 168, 607, 1672, 1912, 2119
NspV TTCGAA 1 cut(s) 902
OliI CACNNNNGTG 1 cut(s) 2201
PaeI GCATGC 2 cut(s) 168, 1912
PaeR7I CTCGAG 1 cut(s) 392
PagI TCATGA 1 cut(s) 2725
PceI AGGCCT 1 cut(s) 12
PciI ACATGT 1 cut(s) 2115
PctI GAATGC 2 cut(s) 1207, 1480
PdiI GCCGGC 2 cut(s) 579, 1091
PdmI GAANNNNTTC 2 cut(s) 550, 2881
PflMI CCANNNNNTGG 1 cut(s) 1003
Ple19I CGATCG 1 cut(s) 72
PleI GAGTC 5 cut(s) 1414, 1435, 2393, 3058, 3060
PpsI GAGTC 5 cut(s) 1414, 1435, 2393, 3058, 3060
Ppu21I YACGTR 1 cut(s) 1587
PpuMI RGGWCCY 1 cut(s) 2135
PscI ACATGT 1 cut(s) 2115
PshBI ATTAAT 1 cut(s) 2334
Psp124BI GAGCTC 2 cut(s) 222, 325
Psp5II RGGWCCY 1 cut(s) 2135
Psp6I CCWGG 4 cut(s) 617, 1196, 1485, 1824
PspFI CCCAGC 1 cut(s) 635
PspGI CCWGG 4 cut(s) 617, 1196, 1485, 1824
PspPI GGNCC 5 cut(s) 583, 1822, 2135, 2193, 2275
PspPPI RGGWCCY 1 cut(s) 2135
PspXI VCTCGAGB 1 cut(s) 392
PstI CTGCAG 1 cut(s) 627
PstNI CAGNNNCTG 1 cut(s) 345
PsuI RGATCY 3 cut(s) 1171, 1245, 2940
PvuI CGATCG 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 635
RsaI GTAC 4 cut(s) 1363, 2000, 2767, 2781
RsaNI GTAC 4 cut(s) 1362, 1999, 2766, 2780
RseI CAYNNNNRTG 4 cut(s) 1667, 2201, 2730, 3128
SacI GAGCTC 2 cut(s) 222, 325
SacII CCGCGG 2 cut(s) 519, 543
Sau96I GGNCC 5 cut(s) 583, 1822, 2135, 2193, 2275
ScaI AGTACT 1 cut(s) 2781
SchI GAGTC 5 cut(s) 1415, 1436, 2393, 3059, 3060
SduI GDGCHC 8 cut(s) 20, 214, 222, 272, 325, 738, 798, 1741
SexAI ACCWGGT 1 cut(s) 1824
SfaNI GCATC 6 cut(s) 177, 1318, 1966, 2617, 2800, 3132
SfcI CTRYAG 2 cut(s) 623, 1175
Sfr274I CTCGAG 1 cut(s) 392
Sfr303I CCGCGG 2 cut(s) 519, 543
SfuI TTCGAA 1 cut(s) 902
SgrBI CCGCGG 2 cut(s) 519, 543
SinI GGWCC 4 cut(s) 1822, 2135, 2193, 2275
SlaI CTCGAG 1 cut(s) 392
SmaI CCCGGG 1 cut(s) 274
SmiMI CAYNNNNRTG 4 cut(s) 1667, 2201, 2730, 3128
SmlI CTYRAG 2 cut(s) 392, 2324
SmoI CTYRAG 2 cut(s) 392, 2324
SphI GCATGC 2 cut(s) 168, 1912
SseBI AGGCCT 1 cut(s) 12
SspI AATATT 1 cut(s) 849
SspMI CTAG 9 cut(s) 374, 462, 693, 1224, 2523, 2619, 2642, 2960, 3032
SstI GAGCTC 2 cut(s) 222, 325
StuI AGGCCT 1 cut(s) 12
StyI CCWWGG 6 cut(s) 876, 1155, 1223, 1304, 2618, 3031
TaaI ACNGT 7 cut(s) 1464, 1493, 2146, 2269, 2719, 2932, 3018
TaiI ACGT 3 cut(s) 1127, 1589, 1786
TaqI TCGA 9 cut(s) 68, 387, 393, 410, 417, 810, 902, 2649, 3069
TaqII GACCGA 1 cut(s) 2292
TatI WGTACW 4 cut(s) 1361, 1998, 2765, 2779
TscAI CASTG 9 cut(s) 209, 219, 235, 1188, 1469, 1855, 2149, 2452, 2835
TseFI GTSAC 3 cut(s) 745, 1846, 3037
Tsp45I GTSAC 3 cut(s) 745, 1846, 3037
TspGWI ACGGA 7 cut(s) 746, 1178, 1266, 1351, 1835, 2539, 3156
TspMI CCCGGG 1 cut(s) 272
TspRI CASTG 9 cut(s) 209, 219, 235, 1188, 1469, 1855, 2149, 2452, 2835
Van91I CCANNNNNTGG 1 cut(s) 1003
VpaK11BI GGWCC 4 cut(s) 1822, 2135, 2193, 2275
VspI ATTAAT 1 cut(s) 2334
XapI RAATTY 3 cut(s) 547, 640, 2467
XceI RCATGY 5 cut(s) 168, 607, 1672, 1912, 2119
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XhoI CTCGAG 1 cut(s) 392
XmaI CCCGGG 1 cut(s) 272
XmaJI CCTAGG 3 cut(s) 1223, 2618, 3031
XmnI GAANNNNTTC 2 cut(s) 550, 2881
XspI CTAG 9 cut(s) 374, 462, 693, 1224, 2523, 2619, 2642, 2960, 3032
ZrmI AGTACT 1 cut(s) 2781
Zsp2I ATGCAT 2 cut(s) 170, 1878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.