Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0013028

Physical Location & Seq

Genomic Coordinates Forward (+)

33117760 .. 33122096

Gene Structure 4337 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1264200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3198 bp

ATGAGAGCGGGGCTAAGTACGATCCAGCAAACTTTAACCCCAGAGGCGGCAAGCGTCTTGAACCACTCGATCGCCGAAGCGGGTCGACGGAACCACGGCCAGACGACGCCGCTACACGTGGCGGCAACCCTTTTGTCCTCCCCCACCGGCTTCCTCCGCCAAGCATGTATCAAATCTCACCCCAATTCCTCCCACCCTCTGCAGTGCCGTGCTCTGGAGCTCTGCTTCAGTGTGGCGCTGGAGCGGTTGCCCACCGCCCAAAACATGAGCCCCGGCATGGAGCCCCCCATCTCTAACGCTCTCATGGCTGCCTTGAAGCGAGCTCAGGCTCACCAGCGCCGGGGCTGCCCCGAACAGCAGCAGCAACCTCTCTTAGCAGTCAAAGTTGAGCTCGAGCAGCTCATCATCTCAATTCTCGATGACCCGAGTGTTAGTCGGGTCATGCGGGAGGCTAGTTTCTCTAGTCCTGCAGTCAAAGCCACCATTGAACAAACACTCAACTCTTCTGCCGCCTCTGCCGCTGCCTCCGCCGCCGTCAATTCTTCCCCAATTGGATTGCAGTTCCGCCCCGCTGGCCCCACTGTGCCACCCGCGAGCCGGAATTTGTATTTGAATCCGAGGCTGCAGCAGCCCCAGGGAGCTGCAGCACAGTCGGGACAACGTAGAGGGGAGGAGGTTAAGAGGGTTGGTGATATTTTGTTGAGAACGAAGAAGAGGAACCCAGTTTTGGTTGGCGATTCAGAGCCTGAGGCGGTGACCAAAGAGCTCTTGAGGAGGATTCAGAGCAAGGAATTGGGCGAGGGGCCGCTGAAGAATGTTGAGGTCCTTTATTTGGAGAAGGAGGTTTCTTTGGATAGGAATCAGGTAGTGTCAAAAATGAAGGAATTAGGGAGTTTGATTGAGACTCGGATGTCGAATTCGAATGGGGGAGGGGTCATTCTTGATTTGGGTGACTTGAAATGGCTTGTGGAGCAGACTGCGAGCTTTGGAGTTGCAGCTCCGGGTCTAGGTTCGCCTCCTGTCCAGCAGCAGGTGGTTTCGGAGACAGGGCGGGCAGCAGTGGCGGAAATGGGGAAGCTGTTGGCGAGGTTTGGAGACGGTAGTGCTAATGGCAGCCGGCTTTGGTTAATTGGGACTGCTACTTGTGAGACATATTTGAGGTGCCAAGTCTATCACCCTTCAATGGAAACTGATTGGGATTTACAGGTAGTGCCAATTACTGGCAGAACGCCGCCTTCAGGATTGTTTCCGAGGATGGGGGCGACTAACGGGATCCTTAGTACCTCTGTTGGATCATTGTCACCAATGAAAGGTTTTCCACCAGCATCAATTGATCAACCAAGACTTTTGTCAGAGAACTCGGATCCTGCTCGAAGAGCACCATGTTGCCCTCAATGTACACATAGGTACGAGCAAGAACTTGCAAAACTGGTGGCCAAGGAATCTGAGACATCATCTTCTGAAACGGAGGCATCTCAACCATTGCTACCACAGTGGTTGCAACATGCTAAAGCCCGTGATGTTCATTCCAGTACATTAGATCAGACGCAGACTAAAGATCAAAATTTGATCCTGAAGCAGAAGACTCAAGAATTACAGAAGGAATGGGCAGATACGTGTTTGCGTCTTCATCCTAATTTTCATCAGCCTAGTTTCAGCTCAGAGAGAATTATTCCAACCCTCTCAATGACGGGCTTGTACAGTCCAAACTTGCTTGGTCGCCAACCATTCCAACCCAAATCACATCTGAATAAGAATCTTGGGACTCTGCAATTGAACACAAATCTGCTGACCAGCCAACCATCTGAACGGGCAATTTCCCAACCAGAAAGCCCTGTAAGGACAGAGCTGGTTCTTGGCCAAACAGACGTCACTGAAACCACCCCTGAGCAAGCGCATAAAGAGCGCATCAGAGACTTTATGGGCTGCATGCCTTCTGAACCGCAGAACAAATTACATGGAATGCAAACGGAGGATAACCAATTGTGTCAAATAGATACTGAATCATTCAAAAAGCTCTACAAAGGTTTAATGGAGGTGTGGTGGCAGCAAGATGCAGCAACTGCTGTGGCTGAAACTGTGACAAAATGCAAGTTGGGTAATGGAAAGAGACATGGTGCCGGATCAAGGGGAGACATATGGCTGCTGTTTATGGGCCCGGACAGTGTTGGCAAGAAGAAGATGGCTTCAGCTCTTTCAGAACTGGTATATGGGTCCAACCCAGTGATGATAAGTCTTAGCTCCCAACGTGGTAATTTGCAATCAGATATGAGTTTCCGCGGGAAAACAGTGGTGGATCGAATAGCAGAGACAGTTAAGAGAAACCCTTTTTCAGTAGTAGTGCTTGAGGACATTAATGAAGCTGATATGATAGTTCGTGGTAGCATAAAACGGGCAATTGAGAGAGGTCGTCTTGCTGACTCTTATGGCCGTGAAATCAGTCTTGGAAATGTTGTCTTCATCCTTACAGCAAATTGGTTGCCAGAAAATCTCAGACCCTTATCAAACAACAATTCACTTGAAGAAAAGCTTGCCAGTATAGCGAGAAATGGTTGGCAGTTAAAGCTATCGGTTTGTGCAAGGGCTGCAAAACGCCGAGCCAATTGGTTAACGGATGAAGACAGGGTTACAAAACCTAGGACAGATACGGGTTTAGCATTAGGATTCGATCTGAATGAAGCTGCCAATGCTGAGGATGACAGGACAGATGGCTCGCTTAATTCAAGTGATCTGACGGTTGATCATGAAGATGACAATCGTCTCAATAACAGAGCCTTACTCAAAGTCACAACCCCGTCAGTACCCCGGGAATTGCTGGATACGGTTGATGATGCCATTGTGTTCAAGCCAATAGATTTCAACCCCATTCAGCAAAACATCACAAACACCATCAGAAAAAGATTCTCAAAGATAATGGGTGAGGGGATTTCATTTGAATTGCCAGAGGATGCTGTTGAAAAAATCTTGACTGGAATATGGCTAGGCCGGACTGGATTAGAGGAATGGGCAGAAAAGGTTCTTGCTCCGAGCATCCAGCAGCTTAAGTCCTGCCTTGGTGGCAGCACCGGTGTGATAGCCGACGAGTCAATGGTTGTTAGACTTGAATCCGATGGTGCTTCGGACTGTGGGAGCACCGGAGATAGGCTGCCCAGTAGCATCAATGTGGTTGTGGTGCCTGATGAGTTGAGACAACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007275 GO:0007623 GO:0008150 GO:0009628 GO:0009719 GO:0009725 GO:0009791 GO:0009845 GO:0010033 GO:0014070 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0048511 GO:0048856 GO:0050896 GO:0080167 GO:0090351 GO:1901700 GO:1902347

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_2 Clp_N

Protein Analysis

1066

Amino Acids

116.48

Weight (kDa)

6.82

Isoelectric Point (pI)

47.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	12 - 113	1.1e-06	Clp repeat (R) N-terminal domain
NBD_SMAX1	PF23569	223 - 408	1.9e-32	SMAX1 nucleotide binding domain
AAA_2	PF07724	715 - 831	1.3e-08	AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1	PF26587	952 - 1038	3.3e-23	SMAX1-like, AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013028)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57710
fragaria_vesca	FvH4_4g09820
malus_domestica	MD13G1261900.v1.1 MD16G1264200.v1.1
prunus_persica	Prupe.1G095200_v2.0.a1 Prupe.1G095200_v2.0.a1
pyrus_communis	pycom13g24720 pycom16g23290
rosa_chinensis	RchiOBHm_Chr4g0404601
rosa_laevigata	RLG00000008920
rosa_multiflora	Rmu_sc0002699.1_g000006
rosa_roxburghii	Rroxscaffold_5G00348750
rosa_rugosa	Rorug04G0047700
rosa_samantha	Rh4AG123800 Rh4BG117200 Rh4CG131900 Rh4DG116700
rosa_wichuraiana	Rw4G010030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1021
AasI	GACNNNNNNGTC	1 cut(s)	910
AatII	GACGTC	1 cut(s)	1872
Acc36I	ACCTGC	1 cut(s)	1021
AccB1I	GGYRCC	3 cut(s)	1161, 2117, 3172
AccB7I	CCANNNNNTGG	1 cut(s)	1220
AccBSI	CCGCTC	2 cut(s)	8, 244
AccI	GTMKAC	1 cut(s)	85
AccII	CGCG	2 cut(s)	593, 2280
AclWI	GGATC	9 cut(s)	16, 1267, 1280, 1300, 1358, 1371, 1564, 2131, 2304
AcoI	YGGCCR	4 cut(s)	97, 1434, 1858, 2428
AcsI	RAATTY	3 cut(s)	601, 916, 1564
AcuI	CTGAAG	5 cut(s)	211, 830, 1221, 1595, 2172
AcvI	CACGTG	1 cut(s)	118
AcyI	GRCGYC	2 cut(s)	107, 1869
AfaI	GTAC	7 cut(s)	19, 1282, 1399, 1409, 1534, 1700, 2802
AflII	CTTAAG	1 cut(s)	3041
AflIII	ACRYGT	2 cut(s)	115, 1616
AgeI	ACCGGT	1 cut(s)	3065
AjnI	CCWGG	1 cut(s)	633
AjuI	GAANNNNNNNTTGG	4 cut(s)	710, 742, 2427, 2459
AleI	CACNNNNGTG	1 cut(s)	3068
Alw21I	GWGCWC	7 cut(s)	214, 222, 325, 393, 768, 1381, 3134
AlwI	GGATC	9 cut(s)	16, 1267, 1280, 1300, 1358, 1371, 1564, 2131, 2304
AlwNI	CAGNNNCTG	2 cut(s)	746, 2063
Ama87I	CYCGRG	3 cut(s)	392, 424, 2805
AoxI	GGCC	8 cut(s)	97, 574, 803, 1434, 1858, 2155, 2428, 2983
ApaI	GGGCCC	1 cut(s)	2159
ApoI	RAATTY	3 cut(s)	601, 916, 1564
AseI	ATTAAT	1 cut(s)	2355
AsiGI	ACCGGT	1 cut(s)	3065
Asp700I	GAANNNNTTC	1 cut(s)	3015
AspA2I	CCTAGG	1 cut(s)	2636
AspLEI	GCGC	4 cut(s)	238, 339, 1897, 1908
AspS9I	GGNCC	6 cut(s)	575, 803, 823, 2155, 2156, 2214
AsuC2I	CCSGG	6 cut(s)	273, 341, 1002, 2159, 2806, 2807
AsuHPI	GGTGA	8 cut(s)	170, 323, 701, 766, 962, 1166, 1293, 2930
AsuII	TTCGAA	1 cut(s)	920
AvaI	CYCGRG	3 cut(s)	392, 424, 2805
AvaII	GGWCC	2 cut(s)	823, 2214
AvrII	CCTAGG	1 cut(s)	2636
AxyI	CCTNAGG	1 cut(s)	747
BaeGI	GKGCMC	1 cut(s)	2159
BalI	TGGCCA	2 cut(s)	1436, 1860
BamHI	GGATCC	2 cut(s)	1272, 1363
BanI	GGYRCC	3 cut(s)	1161, 2117, 3172
BanII	GRGCYC	7 cut(s)	222, 272, 285, 325, 393, 768, 2159
BbrPI	CACGTG	1 cut(s)	118
BbsI	GAAGAC	4 cut(s)	1589, 1619, 2449, 2625
Bbv12I	GWGCWC	7 cut(s)	214, 222, 325, 393, 768, 1381, 3134
BbvCI	CCTCAGC	1 cut(s)	2691
BccI	CCATC	7 cut(s)	296, 1249, 1810, 2176, 2702, 2897, 3104
BceAI	ACGGC	4 cut(s)	112, 192, 518, 2415
BcgI	CGANNNNNNTGC	2 cut(s)	633, 667
BciT130I	CCWGG	1 cut(s)	635
BciVI	GTATCC	1 cut(s)	2812
BclI	TGATCA	2 cut(s)	1333, 2740
BcnI	CCSGG	6 cut(s)	273, 341, 1002, 2159, 2806, 2807
BfaI	CTAG	6 cut(s)	453, 462, 1007, 1650, 2637, 2981
BfmI	CTRYAG	4 cut(s)	200, 468, 623, 642
BfoI	RGCGCY	2 cut(s)	239, 340
BfrI	CTTAAG	1 cut(s)	3041
BfuAI	ACCTGC	1 cut(s)	1021
BfuI	GTATCC	1 cut(s)	2812
BglI	GCCNNNNNGGC	2 cut(s)	573, 3057
BlnI	CCTAGG	1 cut(s)	2636
Bme1390I	CCNGG	7 cut(s)	273, 341, 635, 1002, 2159, 2806, 2807
Bme18I	GGWCC	2 cut(s)	823, 2214
BmeT110I	CYCGRG	3 cut(s)	392, 424, 2805
BmgT120I	GGNCC	6 cut(s)	575, 803, 823, 2155, 2156, 2214
BmrFI	CCNGG	7 cut(s)	273, 341, 635, 1002, 2159, 2806, 2807
BmrI	ACTGGG	3 cut(s)	716, 2216, 3144
BmsI	GCATC	8 cut(s)	1334, 1481, 1917, 2044, 2821, 2938, 3039, 3165
BmuI	ACTGGG	3 cut(s)	716, 2216, 3144
BoxI	GACNNNNGTC	2 cut(s)	1348, 2757
BpiI	GAAGAC	4 cut(s)	1589, 1619, 2449, 2625
BplI	GAGNNNNNCTC	2 cut(s)	2763, 2795
BpmI	CTGGAG	2 cut(s)	236, 260
Bpu10I	CCTNAGC	3 cut(s)	324, 1887, 2691
Bpu14I	TTCGAA	1 cut(s)	920
BpuEI	CTTGAG	3 cut(s)	790, 1572, 2366
BpuMI	CCSGG	6 cut(s)	273, 341, 1002, 2159, 2806, 2807
BsaAI	YACGTR	2 cut(s)	118, 1617
BsaBI	GATNNNNATC	2 cut(s)	858, 2754
BsaHI	GRCGYC	2 cut(s)	107, 1869
BsaWI	WCCGGW	2 cut(s)	3065, 3134
BsaXI	ACNNNNNCTCC	2 cut(s)	2027, 2057
Bse118I	RCCGGY	3 cut(s)	146, 1116, 3065
Bse21I	CCTNAGG	1 cut(s)	747
Bse3DI	GCAATG	1 cut(s)	1481
Bse8I	GATNNNNATC	2 cut(s)	858, 2754
BseBI	CCWGG	1 cut(s)	635
BseGI	GGATG	8 cut(s)	915, 1260, 1630, 2460, 2620, 2701, 2953, 3030
BseJI	GATNNNNATC	2 cut(s)	858, 2754
BseMI	GCAATG	1 cut(s)	1481
BseMII	CTCAG	7 cut(s)	338, 738, 1437, 1674, 1878, 2506, 2682
BseRI	GAGGAG	2 cut(s)	686, 787
BseSI	GKGCMC	1 cut(s)	2159
Bsh1236I	CGCG	2 cut(s)	593, 2280
Bsh1285I	CGRYCG	1 cut(s)	72
BshFI	GGCC	8 cut(s)	99, 576, 805, 1436, 1860, 2157, 2430, 2985
BshNI	GGYRCC	3 cut(s)	1161, 2117, 3172
BshTI	ACCGGT	1 cut(s)	3065
BsiEI	CGRYCG	1 cut(s)	72
BsiHKAI	GWGCWC	7 cut(s)	214, 222, 325, 393, 768, 1381, 3134
BsiHKCI	CYCGRG	3 cut(s)	392, 424, 2805
BslFI	GGGAC	3 cut(s)	669, 1147, 1777
BsmBI	CGTCTC	2 cut(s)	1089, 2765
BsmFI	GGGAC	3 cut(s)	669, 1147, 1777
BsmI	GAATGC	1 cut(s)	1968
BsnI	GGCC	8 cut(s)	99, 576, 805, 1436, 1860, 2157, 2430, 2985
BsoBI	CYCGRG	3 cut(s)	392, 424, 2805
Bsp119I	TTCGAA	1 cut(s)	920
Bsp120I	GGGCCC	1 cut(s)	2155
Bsp1407I	TGTACA	2 cut(s)	1397, 1698
BspANI	GGCC	8 cut(s)	99, 576, 805, 1436, 1860, 2157, 2430, 2985
BspCNI	CTCAG	7 cut(s)	337, 739, 1438, 1673, 1879, 2505, 2683
BspFNI	CGCG	2 cut(s)	593, 2280
BspHI	TCATGA	1 cut(s)	2743
BspMAI	CTGCAG	4 cut(s)	204, 472, 627, 646
BspMI	ACCTGC	1 cut(s)	1021
BspPI	GGATC	9 cut(s)	16, 1267, 1280, 1300, 1358, 1371, 1564, 2131, 2304
BspQI	GCTCTTC	1 cut(s)	1369
BspT104I	TTCGAA	1 cut(s)	920
BspT107I	GGYRCC	3 cut(s)	1161, 2117, 3172
BspTI	CTTAAG	1 cut(s)	3041
BsrBI	CCGCTC	2 cut(s)	8, 244
BsrDI	GCAATG	1 cut(s)	1481
BsrFI	RCCGGY	3 cut(s)	146, 1116, 3065
BsrGI	TGTACA	2 cut(s)	1397, 1698
BssAI	RCCGGY	3 cut(s)	146, 1116, 3065
BssNI	GRCGYC	2 cut(s)	107, 1869
BssT1I	CCWWGG	3 cut(s)	1437, 2636, 3052
Bst2UI	CCWGG	1 cut(s)	635
Bst6I	CTCTTC	3 cut(s)	508, 707, 1369
BstACI	GRCGYC	2 cut(s)	107, 1869
BstAFI	CTTAAG	1 cut(s)	3041
BstAPI	GCANNNNNTGC	2 cut(s)	2063, 2585
BstAUI	TGTACA	2 cut(s)	1397, 1698
BstBAI	YACGTR	2 cut(s)	118, 1617
BstBI	TTCGAA	1 cut(s)	920
BstDSI	CCRYGG	2 cut(s)	94, 2278
BstEII	GGTNACC	1 cut(s)	754
BstF5I	GGATG	8 cut(s)	915, 1260, 1630, 2460, 2620, 2701, 2953, 3030
BstFNI	CGCG	2 cut(s)	593, 2280
BstH2I	RGCGCY	2 cut(s)	239, 340
BstHHI	GCGC	4 cut(s)	238, 339, 1897, 1908
BstMCI	CGRYCG	1 cut(s)	72
BstNI	CCWGG	1 cut(s)	635
BstNSI	RCATGY	3 cut(s)	168, 1508, 1933
BstPAI	GACNNNNGTC	2 cut(s)	1348, 2757
BstPI	GGTNACC	1 cut(s)	754
BstSCI	CCNGG	7 cut(s)	271, 339, 633, 1000, 2157, 2804, 2805
BstSFI	CTRYAG	4 cut(s)	200, 468, 623, 642
BstSLI	GKGCMC	1 cut(s)	2159
BstUI	CGCG	2 cut(s)	593, 2280
BstV2I	GAAGAC	4 cut(s)	1589, 1619, 2449, 2625
BstX2I	RGATCY	2 cut(s)	1272, 1363
BstYI	RGATCY	2 cut(s)	1272, 1363
Bsu36I	CCTNAGG	1 cut(s)	747
BsuI	GTATCC	1 cut(s)	2812
BsuRI	GGCC	8 cut(s)	99, 576, 805, 1436, 1860, 2157, 2430, 2985
BtgI	CCRYGG	2 cut(s)	94, 2278
BtsCI	GGATG	8 cut(s)	915, 1260, 1630, 2460, 2620, 2701, 2953, 3030
BtsI	GCAGTG	2 cut(s)	209, 1065
BtsIMutI	CAGTG	9 cut(s)	209, 235, 579, 1065, 1499, 1872, 2170, 2229, 2295
BveI	ACCTGC	1 cut(s)	1021
CaiI	CAGNNNCTG	2 cut(s)	746, 2063
CciI	TCATGA	1 cut(s)	2743
CfoI	GCGC	4 cut(s)	238, 339, 1897, 1908
Cfr10I	RCCGGY	3 cut(s)	146, 1116, 3065
Cfr13I	GGNCC	6 cut(s)	575, 803, 823, 2155, 2156, 2214
Cfr42I	CCGCGG	1 cut(s)	2281
Cfr9I	CCCGGG	1 cut(s)	2805
CseI	GACGC	4 cut(s)	43, 115, 1555, 1613
Csp6I	GTAC	7 cut(s)	18, 1281, 1398, 1408, 1533, 1699, 2801
CspAI	ACCGGT	1 cut(s)	3065
CspCI	CAANNNNNGTGG	6 cut(s)	1413, 1448, 1479, 1514, 2049, 2084
CviQI	GTAC	7 cut(s)	18, 1281, 1398, 1408, 1533, 1699, 2801
DrdI	GACNNNNNNGTC	1 cut(s)	910
DseDI	GACNNNNNNGTC	1 cut(s)	910
EaeI	YGGCCR	4 cut(s)	97, 1434, 1858, 2428
Eam1104I	CTCTTC	3 cut(s)	508, 707, 1369
EarI	CTCTTC	3 cut(s)	508, 707, 1369
EciI	GGCGGA	4 cut(s)	146, 517, 554, 1079
Ecl136II	GAGCTC	4 cut(s)	220, 323, 391, 766
Eco130I	CCWWGG	3 cut(s)	1437, 2636, 3052
Eco24I	GRGCYC	7 cut(s)	222, 272, 285, 325, 393, 768, 2159
Eco47I	GGWCC	2 cut(s)	823, 2214
Eco53kI	GAGCTC	4 cut(s)	220, 323, 391, 766
Eco57I	CTGAAG	5 cut(s)	211, 830, 1221, 1595, 2172
Eco72I	CACGTG	1 cut(s)	118
Eco81I	CCTNAGG	1 cut(s)	747
Eco88I	CYCGRG	3 cut(s)	392, 424, 2805
Eco91I	GGTNACC	1 cut(s)	754
EcoICRI	GAGCTC	4 cut(s)	220, 323, 391, 766
EcoO109I	RGGNCCY	1 cut(s)	823
EcoO65I	GGTNACC	1 cut(s)	754
EcoRI	GAATTC	1 cut(s)	916
EcoRII	CCWGG	1 cut(s)	633
EcoT14I	CCWWGG	3 cut(s)	1437, 2636, 3052
EcoT38I	GRGCYC	7 cut(s)	222, 272, 285, 325, 393, 768, 2159
ErhI	CCWWGG	3 cut(s)	1437, 2636, 3052
Esp3I	CGTCTC	2 cut(s)	1089, 2765
FalI	AAGNNNNNCTT	2 cut(s)	2514, 2546
FaqI	GGGAC	3 cut(s)	669, 1147, 1777
FauI	CCCGC	6 cut(s)	73, 438, 577, 598, 1044, 2273
FauNDI	CATATG	1 cut(s)	2138
FbaI	TGATCA	2 cut(s)	1333, 2740
FblI	GTMKAC	1 cut(s)	85
FokI	GGATG	8 cut(s)	922, 1267, 1617, 2447, 2627, 2708, 2960, 3017
FriOI	GRGCYC	7 cut(s)	222, 272, 285, 325, 393, 768, 2159
FspBI	CTAG	6 cut(s)	453, 462, 1007, 1650, 2637, 2981
GlaI	GCGC	4 cut(s)	237, 338, 1896, 1907
GsuI	CTGGAG	2 cut(s)	236, 260
HaeII	RGCGCY	2 cut(s)	239, 340
HaeIII	GGCC	8 cut(s)	99, 576, 805, 1436, 1860, 2157, 2430, 2985
HgaI	GACGC	4 cut(s)	43, 115, 1555, 1613
HhaI	GCGC	4 cut(s)	238, 339, 1897, 1908
Hin1I	GRCGYC	2 cut(s)	107, 1869
Hin6I	GCGC	4 cut(s)	236, 337, 1895, 1906
HinP1I	GCGC	4 cut(s)	236, 337, 1895, 1906
HincII	GTYRAC	2 cut(s)	86, 2610
HindII	GTYRAC	2 cut(s)	86, 2610
HindIII	AAGCTT	1 cut(s)	2528
HpaI	GTTAAC	1 cut(s)	2610
HphI	GGTGA	8 cut(s)	170, 323, 701, 766, 962, 1166, 1293, 2930
Hpy166II	GTNNAC	3 cut(s)	86, 1400, 2610
Hpy8I	GTNNAC	3 cut(s)	86, 1400, 2610
Hpy99I	CGWCG	3 cut(s)	90, 109, 3083
HpyAV	CCTTC	6 cut(s)	832, 874, 1188, 1245, 1594, 1944
HpyCH4IV	ACGT	5 cut(s)	117, 661, 1616, 1869, 2248
HpySE526I	ACGT	5 cut(s)	117, 661, 1616, 1869, 2248
Hsp92I	GRCGYC	2 cut(s)	107, 1869
HspAI	GCGC	4 cut(s)	236, 337, 1895, 1906
KroI	GCCGGC	1 cut(s)	1116
KroNI	GCCGGC	1 cut(s)	1118
Ksp22I	TGATCA	2 cut(s)	1333, 2740
KspAI	GTTAAC	1 cut(s)	2610
KspI	CCGCGG	1 cut(s)	2281
LguI	GCTCTTC	1 cut(s)	1369
LmnI	GCTCC	9 cut(s)	217, 241, 280, 638, 970, 1003, 2246, 3028, 3129
LweI	GCATC	8 cut(s)	1334, 1481, 1917, 2044, 2821, 2938, 3039, 3165
MaeI	CTAG	6 cut(s)	453, 462, 1007, 1650, 2637, 2981
MaeII	ACGT	5 cut(s)	117, 661, 1616, 1869, 2248
MaeIII	GTNAC	7 cut(s)	754, 950, 1299, 1870, 2080, 2626, 2785
MbiI	CCGCTC	2 cut(s)	8, 244
MfeI	CAATTG	6 cut(s)	549, 1329, 1772, 1982, 2397, 2602
MflI	RGATCY	2 cut(s)	1272, 1363
MlsI	TGGCCA	2 cut(s)	1436, 1860
MluNI	TGGCCA	2 cut(s)	1436, 1860
MlyI	GAGTC	5 cut(s)	898, 1579, 1759, 2414, 3092
MmeI	TCCRAC	4 cut(s)	1270, 1700, 1756, 2241
Mox20I	TGGCCA	2 cut(s)	1436, 1860
MroNI	GCCGGC	1 cut(s)	1116
MroXI	GAANNNNTTC	1 cut(s)	3015
MscI	TGGCCA	2 cut(s)	1436, 1860
MslI	CAYNNNNRTG	3 cut(s)	2748, 3068, 3161
Msp20I	TGGCCA	2 cut(s)	1436, 1860
MspA1I	CMGCKG	4 cut(s)	521, 572, 808, 2280
MspCI	CTTAAG	1 cut(s)	3041
MspR9I	CCNGG	7 cut(s)	273, 341, 635, 1002, 2159, 2806, 2807
MunI	CAATTG	6 cut(s)	549, 1329, 1772, 1982, 2397, 2602
Mva1269I	GAATGC	1 cut(s)	1968
MvaI	CCWGG	1 cut(s)	635
MvnI	CGCG	2 cut(s)	593, 2280
NaeI	GCCGGC	1 cut(s)	1118
NciI	CCSGG	6 cut(s)	273, 341, 1002, 2159, 2806, 2807
NdeI	CATATG	1 cut(s)	2138
NgoMIV	GCCGGC	1 cut(s)	1116
NmeAIII	GCCGAG	1 cut(s)	2621
NmuCI	GTSAC	6 cut(s)	754, 950, 1299, 1870, 2080, 2785
NspI	RCATGY	3 cut(s)	168, 1508, 1933
NspV	TTCGAA	1 cut(s)	920
OliI	CACNNNNGTG	1 cut(s)	3068
PaeI	GCATGC	1 cut(s)	1933
PaeR7I	CTCGAG	1 cut(s)	392
PagI	TCATGA	1 cut(s)	2743
PaqCI	CACCTGC	1 cut(s)	1021
PasI	CCCWGGG	1 cut(s)	634
PciSI	GCTCTTC	1 cut(s)	1369
PctI	GAATGC	1 cut(s)	1968
PdiI	GCCGGC	1 cut(s)	1118
PdmI	GAANNNNTTC	1 cut(s)	3015
PflMI	CCANNNNNTGG	1 cut(s)	1220
PinAI	ACCGGT	1 cut(s)	3065
Ple19I	CGATCG	1 cut(s)	72
PleI	GAGTC	5 cut(s)	898, 1579, 1759, 2414, 3091
PmaCI	CACGTG	1 cut(s)	118
PmlI	CACGTG	1 cut(s)	118
PpsI	GAGTC	5 cut(s)	898, 1579, 1759, 2414, 3091
Ppu21I	YACGTR	2 cut(s)	118, 1617
PpuMI	RGGWCCY	1 cut(s)	823
PshAI	GACNNNNGTC	2 cut(s)	1348, 2757
PshBI	ATTAAT	1 cut(s)	2355
Psp124BI	GAGCTC	4 cut(s)	222, 325, 393, 768
Psp5II	RGGWCCY	1 cut(s)	823
Psp6I	CCWGG	1 cut(s)	633
PspCI	CACGTG	1 cut(s)	118
PspEI	GGTNACC	1 cut(s)	754
PspGI	CCWGG	1 cut(s)	633
PspOMI	GGGCCC	1 cut(s)	2155
PspPI	GGNCC	6 cut(s)	575, 803, 823, 2155, 2156, 2214
PspPPI	RGGWCCY	1 cut(s)	823
PspXI	VCTCGAGB	1 cut(s)	392
PstI	CTGCAG	4 cut(s)	204, 472, 627, 646
PstNI	CAGNNNCTG	2 cut(s)	746, 2063
PsuI	RGATCY	2 cut(s)	1272, 1363
PvuI	CGATCG	1 cut(s)	72
RsaI	GTAC	7 cut(s)	19, 1282, 1399, 1409, 1534, 1700, 2802
RsaNI	GTAC	7 cut(s)	18, 1281, 1398, 1408, 1533, 1699, 2801
RseI	CAYNNNNRTG	3 cut(s)	2748, 3068, 3161
SacI	GAGCTC	4 cut(s)	222, 325, 393, 768
SacII	CCGCGG	1 cut(s)	2281
SalI	GTCGAC	1 cut(s)	84
SapI	GCTCTTC	1 cut(s)	1369
Sau96I	GGNCC	6 cut(s)	575, 803, 823, 2155, 2156, 2214
SchI	GAGTC	5 cut(s)	898, 1579, 1759, 2414, 3092
ScrFI	CCNGG	7 cut(s)	273, 341, 635, 1002, 2159, 2806, 2807
SfaNI	GCATC	8 cut(s)	1334, 1481, 1917, 2044, 2821, 2938, 3039, 3165
SfcI	CTRYAG	4 cut(s)	200, 468, 623, 642
Sfr274I	CTCGAG	1 cut(s)	392
Sfr303I	CCGCGG	1 cut(s)	2281
SfuI	TTCGAA	1 cut(s)	920
SgrAI	CRCCGGYG	1 cut(s)	3065
SgrBI	CCGCGG	1 cut(s)	2281
SinI	GGWCC	2 cut(s)	823, 2214
SlaI	CTCGAG	1 cut(s)	392
SmaI	CCCGGG	1 cut(s)	2807
SmiMI	CAYNNNNRTG	3 cut(s)	2748, 3068, 3161
SmlI	CTYRAG	5 cut(s)	392, 769, 1587, 2345, 3041
SmoI	CTYRAG	5 cut(s)	392, 769, 1587, 2345, 3041
SphI	GCATGC	1 cut(s)	1933
SspMI	CTAG	6 cut(s)	453, 462, 1007, 1650, 2637, 2981
SstI	GAGCTC	4 cut(s)	222, 325, 393, 768
StyD4I	CCNGG	7 cut(s)	271, 339, 633, 1000, 2157, 2804, 2805
StyI	CCWWGG	3 cut(s)	1437, 2636, 3052
TaiI	ACGT	5 cut(s)	120, 664, 1619, 1872, 2251
TaqI	TCGA	9 cut(s)	68, 85, 393, 417, 914, 920, 1372, 2299, 2667
TatI	WGTACW	3 cut(s)	1397, 1532, 1698
TauI	GCSGC	8 cut(s)	50, 112, 125, 512, 521, 533, 808, 1234
TscAI	CASTG	9 cut(s)	209, 235, 586, 1065, 1499, 1879, 2170, 2229, 2295
TseFI	GTSAC	6 cut(s)	754, 950, 1299, 1870, 2080, 2785
Tsp45I	GTSAC	6 cut(s)	754, 950, 1299, 1870, 2080, 2785
TspGWI	ACGGA	4 cut(s)	103, 1481, 1985, 2627
TspMI	CCCGGG	1 cut(s)	2805
TspRI	CASTG	9 cut(s)	209, 235, 586, 1065, 1499, 1879, 2170, 2229, 2295
Van91I	CCANNNNNTGG	1 cut(s)	1220
Vha464I	CTTAAG	1 cut(s)	3041
VpaK11BI	GGWCC	2 cut(s)	823, 2214
VspI	ATTAAT	1 cut(s)	2355
XapI	RAATTY	3 cut(s)	601, 916, 1564
XceI	RCATGY	3 cut(s)	168, 1508, 1933
XhoI	CTCGAG	1 cut(s)	392
XmaI	CCCGGG	1 cut(s)	2805
XmaJI	CCTAGG	1 cut(s)	2636
XmiI	GTMKAC	1 cut(s)	85
XmnI	GAANNNNTTC	1 cut(s)	3015
XspI	CTAG	6 cut(s)	453, 462, 1007, 1650, 2637, 2981
ZraI	GACGTC	1 cut(s)	1870

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.