FvH4_4g11460

Biotin synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015317
Physical Location & Seq
Forward (+)
15214707 .. 15219021
4315 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g11460.t3

Sequence Viewer

Length: 1167 bp
ATGTTCTCCATTAGGTCCATTCTCCTCCGCCACGGCCTCCGCCCCTCCTCCTCTTCGGCGTCTGTGGCGGCTGTATTGCATTCATCGTTTTACTCCTCTCAATCTTCATCAGCAGCTGCAGTTGAAGCCGAGGGAACAATCCTCCAAGGCCCTCGAAACGACTGGACTCGCGACGAGATAAGAGCCGTCTACGACTCTCCTGTTCTCGATCTCCTCTTCCATGGAGCTCAGGTTCACAGACATGTCCATAATTTTAGAGAAGTGCAGCAGTGCACTCTCCTCTCTATCAAGACTGGTGGCTGTAGTGAGGATTGCTCCTATTGCCCTCAGTCTTCAAGATATGATACCGGACTTAAGGCCCAAAAGCTTATGACCAAGGACGATGTAATGGAGGCAGCTAAAAAGGCAAAGGAGGCTGGTAGCACACGCTTTTGCATGGGTGCTGCCTGGAGGGAGACAGTAGGAAGAAAGACAAACTTTAACCAGATCCTTGAATATGTGACAGAAATAAGGAATTTGGGAATGGAAGTATGCTGCACCTTAGGCATGCTAGAACAGCAGCAAGCGCTAAAACTTAAAGAAGCTGGCCTGACAGCTTATAATCACAATCTAGACACCTCAAGGGAATATTATCCCAATATCATTACCACAAGGACCTATGATGACCGCTTGGAAACAATTAAGTATGTCAGGGATGCAGGAATTAGTGTGTGCTCAGGAGGAATAATAGGGCTCGGAGAAGCAGAGGAGGATCGAGTTGGTTTGTTGCATACATTAGCAACACTTCCATCTCACCCAGAAAGTGTTCCCATAAATGCGCTGATTAGTGTGAAAGGGACGCCTCTTCAGGATCAAAAGCCAGTTGAAATATGGGAGATGATACGCATGATTGCCACAGCACGTATAGTCATGCCAAAGGCAATGGTCAGATTGTCAGCTGGCAGAGTTCGTTTCTCAATGCCTGAGCAGGCACTGTGTTTTCTTGCTGGGGCAAATTCAATATTTACTGGTGAAAAACTATTGACCACACCTAACAATGATTTTGATGCCGATCAAGTTATGTTCAAGGTGCTTGGACTAATTCCAAAACCTCCCACTTTCTCCGATGAAGCAGCAAAGGCATCCGAAGCAGAAGCTTGTGAGGATGCTGTTTCTGTTTCAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
BATS Radical_SAM

Protein Analysis

389

Amino Acids

42.61

Weight (kDa)

6.03

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 96 - 258 1.9e-23 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43360
fragaria_vesca FvH4_4g11460 FvH4_4g11460
malus_domestica MD04G1037300.v1.1
prunus_persica Prupe.1G186700_v2.0.a1
pyrus_communis pycom04g03040
rosa_chinensis RchiOBHm_Chr4g0408131
rosa_laevigata RLG00000008670 RLG00000008673
rosa_multiflora Rmu_sc0002073.1_g000002
rosa_roxburghii Rroxscaffold_5G00352620
rosa_rugosa Rorug04G0083700
rosa_samantha Rh4AG148100 Rh4BG145300 Rh4DG141800
rosa_wichuraiana Rw4G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
AccI GTMKAC 1 cut(s) 189
AccII CGCG 1 cut(s) 171
AciI CCGC 4 cut(s) 28, 40, 68, 667
AclWI GGATC 3 cut(s) 481, 759, 858
AcsI RAATTY 2 cut(s) 514, 994
AcuI CTGAAG 1 cut(s) 830
AcyI GRCGYC 2 cut(s) 59, 839
AfeI AGCGCT 1 cut(s) 567
AflII CTTAAG 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 241
AgsI TTSAA 6 cut(s) 125, 336, 494, 866, 999, 1066
AjnI CCWGG 1 cut(s) 446
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
AluBI AGCT 8 cut(s) 116, 227, 367, 398, 584, 596, 938, 1136
AluI AGCT 8 cut(s) 116, 227, 367, 398, 584, 596, 938, 1136
Alw21I GWGCWC 3 cut(s) 229, 275, 716
Alw26I GTCTC 1 cut(s) 449
Alw44I GTGCAC 1 cut(s) 271
AlwI GGATC 3 cut(s) 481, 759, 858
AlwNI CAGNNNCTG 2 cut(s) 116, 973
Aor51HI AGCGCT 1 cut(s) 567
AoxI GGCC 4 cut(s) 34, 148, 357, 586
ApaLI GTGCAC 1 cut(s) 271
ApeKI GCWGC 8 cut(s) 113, 116, 265, 395, 443, 534, 559, 1112
ApoI RAATTY 2 cut(s) 514, 994
Asp700I GAANNNNTTC 1 cut(s) 804
AspLEI GCGC 2 cut(s) 568, 820
AspS9I GGNCC 4 cut(s) 15, 149, 358, 654
AsuHPI GGTGA 2 cut(s) 785, 1022
AvaII GGWCC 2 cut(s) 15, 654
AxyI CCTNAGG 1 cut(s) 541
BaeGI GKGCMC 1 cut(s) 275
BanII GRGCYC 2 cut(s) 229, 735
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 3 cut(s) 229, 275, 716
BbvI GCAGC 8 cut(s) 103, 125, 277, 407, 430, 521, 571, 1124
BccI CCATC 1 cut(s) 796
BceAI ACGGC 2 cut(s) 49, 170
BciT130I CCWGG 1 cut(s) 448
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 2 cut(s) 551, 611
BfmI CTRYAG 2 cut(s) 117, 301
BfoI RGCGCY 1 cut(s) 569
BfrI CTTAAG 1 cut(s) 353
BisI GCNGC 9 cut(s) 69, 114, 117, 266, 396, 444, 535, 560, 1113
BlsI GCNGC 9 cut(s) 70, 115, 118, 267, 397, 445, 536, 561, 1114
Bme1390I CCNGG 1 cut(s) 448
Bme18I GGWCC 2 cut(s) 15, 654
BmgT120I GGNCC 4 cut(s) 15, 149, 358, 654
BmrFI CCNGG 1 cut(s) 448
BmsI GCATC 4 cut(s) 685, 1036, 1130, 1135
BpiI GAAGAC 1 cut(s) 324
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BpmI CTGGAG 1 cut(s) 469
Bpu10I CCTNAGC 3 cut(s) 228, 715, 963
BpuEI CTTGAG 1 cut(s) 604
BsaAI YACGTR 1 cut(s) 902
BsaBI GATNNNNATC 1 cut(s) 1050
BsaHI GRCGYC 2 cut(s) 59, 839
BsaJI CCNNGG 5 cut(s) 31, 129, 145, 220, 375
BsaWI WCCGGW 1 cut(s) 347
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bse1I ACTGG 4 cut(s) 167, 298, 860, 1012
Bse21I CCTNAGG 1 cut(s) 541
Bse3DI GCAATG 1 cut(s) 927
Bse8I GATNNNNATC 1 cut(s) 1050
BseBI CCWGG 1 cut(s) 448
BseDI CCNNGG 5 cut(s) 31, 129, 145, 220, 375
BseGI GGATG 3 cut(s) 700, 1121, 1150
BseJI GATNNNNATC 1 cut(s) 1050
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 4 cut(s) 242, 341, 729, 954
BseNI ACTGG 4 cut(s) 167, 298, 860, 1012
BseRI GAGGAG 7 cut(s) 14, 37, 40, 85, 203, 269, 761
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 8 cut(s) 103, 125, 277, 407, 430, 521, 571, 1124
BseYI CCCAGC 1 cut(s) 986
BsgI GTGCAG 2 cut(s) 284, 520
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 4 cut(s) 36, 150, 359, 588
BsiHKAI GWGCWC 3 cut(s) 229, 275, 716
BsiSI CCGG 1 cut(s) 348
BslFI GGGAC 1 cut(s) 850
BsmAI GTCTC 1 cut(s) 449
BsmFI GGGAC 1 cut(s) 850
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 4 cut(s) 36, 150, 359, 588
Bsp1286I GDGCHC 4 cut(s) 229, 275, 716, 735
Bsp143I GATC 5 cut(s) 208, 486, 751, 850, 1051
Bsp19I CCATGG 1 cut(s) 220
Bsp68I TCGCGA 1 cut(s) 171
BspACI CCGC 4 cut(s) 28, 40, 68, 667
BspANI GGCC 4 cut(s) 36, 150, 359, 588
BspCNI CTCAG 4 cut(s) 241, 340, 728, 955
BspFNI CGCG 1 cut(s) 171
BspMAI CTGCAG 1 cut(s) 121
BspPI GGATC 3 cut(s) 481, 759, 858
BspTI CTTAAG 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 927
BsrI ACTGG 4 cut(s) 167, 298, 860, 1012
BssECI CCNNGG 5 cut(s) 31, 129, 145, 220, 375
BssMI GATC 5 cut(s) 208, 486, 751, 850, 1051
BssNI GRCGYC 2 cut(s) 59, 839
BssT1I CCWWGG 3 cut(s) 145, 220, 375
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 2 cut(s) 460, 975
Bst6I CTCTTC 3 cut(s) 58, 221, 849
BstACI GRCGYC 2 cut(s) 59, 839
BstAFI CTTAAG 1 cut(s) 353
BstBAI YACGTR 1 cut(s) 902
BstC8I GCNNGC 5 cut(s) 548, 564, 586, 940, 969
BstDEI CTNAG 5 cut(s) 228, 327, 541, 715, 963
BstDSI CCRYGG 2 cut(s) 31, 220
BstF5I GGATG 3 cut(s) 700, 1121, 1150
BstFNI CGCG 1 cut(s) 171
BstH2I RGCGCY 1 cut(s) 569
BstHHI GCGC 2 cut(s) 568, 820
BstKTI GATC 5 cut(s) 211, 489, 754, 853, 1054
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 5 cut(s) 208, 486, 751, 850, 1051
BstNI CCWGG 1 cut(s) 448
BstNSI RCATGY 2 cut(s) 245, 550
BstSCI CCNGG 1 cut(s) 446
BstSFI CTRYAG 2 cut(s) 117, 301
BstSLI GKGCMC 1 cut(s) 275
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 8 cut(s) 103, 125, 277, 407, 430, 521, 571, 1124
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 486
BstYI RGATCY 1 cut(s) 486
Bsu36I CCTNAGG 1 cut(s) 541
BsuRI GGCC 4 cut(s) 36, 150, 359, 588
BtgI CCRYGG 2 cut(s) 31, 220
BtsCI GGATG 3 cut(s) 700, 1121, 1150
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 2 cut(s) 275, 971
BtuMI TCGCGA 1 cut(s) 171
Cac8I GCNNGC 5 cut(s) 548, 564, 586, 940, 969
CaiI CAGNNNCTG 2 cut(s) 116, 973
CfoI GCGC 2 cut(s) 568, 820
Cfr13I GGNCC 4 cut(s) 15, 149, 358, 654
CseI GACGC 2 cut(s) 48, 847
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviAII CATG 6 cut(s) 221, 242, 436, 547, 886, 910
DdeI CTNAG 5 cut(s) 228, 327, 541, 715, 963
DpnI GATC 5 cut(s) 210, 488, 753, 852, 1053
DpnII GATC 5 cut(s) 208, 486, 751, 850, 1051
Eam1104I CTCTTC 3 cut(s) 58, 221, 849
EarI CTCTTC 3 cut(s) 58, 221, 849
EciI GGCGGA 2 cut(s) 17, 29
Ecl136II GAGCTC 1 cut(s) 227
Eco130I CCWWGG 3 cut(s) 145, 220, 375
Eco24I GRGCYC 2 cut(s) 229, 735
Eco47I GGWCC 2 cut(s) 15, 654
Eco47III AGCGCT 1 cut(s) 567
Eco53kI GAGCTC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 830
Eco81I CCTNAGG 1 cut(s) 541
EcoICRI GAGCTC 1 cut(s) 227
EcoO109I RGGNCCY 2 cut(s) 149, 654
EcoRII CCWGG 1 cut(s) 446
EcoT14I CCWWGG 3 cut(s) 145, 220, 375
EcoT38I GRGCYC 2 cut(s) 229, 735
ErhI CCWWGG 3 cut(s) 145, 220, 375
FaeI CATG 6 cut(s) 224, 245, 439, 550, 889, 913
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 1 cut(s) 850
FatI CATG 6 cut(s) 220, 241, 435, 546, 885, 909
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 9 cut(s) 69, 114, 117, 266, 396, 444, 535, 560, 1113
FokI GGATG 3 cut(s) 707, 1108, 1157
FriOI GRGCYC 2 cut(s) 229, 735
Fsp4HI GCNGC 9 cut(s) 69, 114, 117, 266, 396, 444, 535, 560, 1113
FspBI CTAG 2 cut(s) 551, 611
GlaI GCGC 2 cut(s) 567, 819
GluI GCNGC 9 cut(s) 69, 114, 117, 266, 396, 444, 535, 560, 1113
GsaI CCCAGC 1 cut(s) 990
GsuI CTGGAG 1 cut(s) 469
HaeII RGCGCY 1 cut(s) 569
HaeIII GGCC 4 cut(s) 36, 150, 359, 588
HapII CCGG 1 cut(s) 348
HgaI GACGC 2 cut(s) 48, 847
HhaI GCGC 2 cut(s) 568, 820
Hin1I GRCGYC 2 cut(s) 59, 839
Hin1II CATG 6 cut(s) 224, 245, 439, 550, 889, 913
Hin6I GCGC 2 cut(s) 566, 818
HinP1I GCGC 2 cut(s) 566, 818
HindIII AAGCTT 2 cut(s) 365, 1134
HinfI GANTC 2 cut(s) 166, 194
HpaII CCGG 1 cut(s) 348
HphI GGTGA 2 cut(s) 785, 1022
Hpy166II GTNNAC 3 cut(s) 190, 235, 273
Hpy188I TCNGA 4 cut(s) 737, 929, 1105, 1126
Hpy188III TCNNGA 7 cut(s) 170, 206, 289, 336, 611, 717, 848
Hpy8I GTNNAC 3 cut(s) 190, 235, 273
Hpy99I CGWCG 1 cut(s) 176
HpyCH4III ACNGT 2 cut(s) 460, 975
HpyCH4IV ACGT 1 cut(s) 901
HpyCH4V TGCA 8 cut(s) 79, 119, 265, 273, 435, 537, 698, 769
HpyF3I CTNAG 5 cut(s) 228, 327, 541, 715, 963
HpySE526I ACGT 1 cut(s) 901
Hsp92I GRCGYC 2 cut(s) 59, 839
Hsp92II CATG 6 cut(s) 224, 245, 439, 550, 889, 913
HspAI GCGC 2 cut(s) 566, 818
Kzo9I GATC 5 cut(s) 208, 486, 751, 850, 1051
LmnI GCTCC 2 cut(s) 224, 320
Lsp1109I GCAGC 8 cut(s) 103, 125, 277, 407, 430, 521, 571, 1124
LweI GCATC 4 cut(s) 685, 1036, 1130, 1135
MaeI CTAG 2 cut(s) 551, 611
MaeII ACGT 1 cut(s) 901
MaeIII GTNAC 1 cut(s) 499
MalI GATC 5 cut(s) 210, 488, 753, 852, 1053
MboI GATC 5 cut(s) 208, 486, 751, 850, 1051
MboII GAAGA 6 cut(s) 45, 96, 208, 324, 477, 836
MflI RGATCY 1 cut(s) 486
MhlI GDGCHC 4 cut(s) 229, 275, 716, 735
MluCI AATT 6 cut(s) 250, 514, 678, 702, 994, 1080
MlyI GAGTC 2 cut(s) 160, 188
MroXI GAANNNNTTC 1 cut(s) 804
MseI TTAA 4 cut(s) 354, 480, 576, 681
MslI CAYNNNNRTG 1 cut(s) 240
MspA1I CMGCKG 2 cut(s) 116, 938
MspCI CTTAAG 1 cut(s) 353
MspI CCGG 1 cut(s) 348
MspR9I CCNGG 1 cut(s) 448
Mva1269I GAATGC 1 cut(s) 79
MvaI CCWGG 1 cut(s) 448
MvnI CGCG 1 cut(s) 171
NcoI CCATGG 1 cut(s) 220
NdeII GATC 5 cut(s) 208, 486, 751, 850, 1051
NlaIII CATG 6 cut(s) 224, 245, 439, 550, 889, 913
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 1 cut(s) 499
NruI TCGCGA 1 cut(s) 171
NspI RCATGY 2 cut(s) 245, 550
PaeI GCATGC 1 cut(s) 550
PciI ACATGT 1 cut(s) 241
PctI GAATGC 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 804
PkrI GCNGC 9 cut(s) 70, 115, 118, 267, 397, 445, 536, 561, 1114
PleI GAGTC 2 cut(s) 160, 188
PpsI GAGTC 2 cut(s) 160, 188
Ppu21I YACGTR 1 cut(s) 902
PpuMI RGGWCCY 1 cut(s) 654
PscI ACATGT 1 cut(s) 241
PsiI TTATAA 1 cut(s) 600
Psp124BI GAGCTC 1 cut(s) 229
Psp5II RGGWCCY 1 cut(s) 654
Psp6I CCWGG 1 cut(s) 446
PspFI CCCAGC 1 cut(s) 986
PspGI CCWGG 1 cut(s) 446
PspPI GGNCC 4 cut(s) 15, 149, 358, 654
PspPPI RGGWCCY 1 cut(s) 654
PstI CTGCAG 1 cut(s) 121
PstNI CAGNNNCTG 2 cut(s) 116, 973
PsuI RGATCY 1 cut(s) 486
PvuII CAGCTG 2 cut(s) 116, 938
RruI TCGCGA 1 cut(s) 171
RseI CAYNNNNRTG 1 cut(s) 240
SacI GAGCTC 1 cut(s) 229
SaqAI TTAA 4 cut(s) 354, 480, 576, 681
SatI GCNGC 9 cut(s) 69, 114, 117, 266, 396, 444, 535, 560, 1113
Sau3AI GATC 5 cut(s) 208, 486, 751, 850, 1051
Sau96I GGNCC 4 cut(s) 15, 149, 358, 654
SchI GAGTC 2 cut(s) 160, 188
ScrFI CCNGG 1 cut(s) 448
SduI GDGCHC 4 cut(s) 229, 275, 716, 735
SfaNI GCATC 4 cut(s) 685, 1036, 1130, 1135
SfcI CTRYAG 2 cut(s) 117, 301
SinI GGWCC 2 cut(s) 15, 654
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 2 cut(s) 353, 619
SmoI CTYRAG 2 cut(s) 353, 619
SphI GCATGC 1 cut(s) 550
Sse9I AATT 6 cut(s) 250, 514, 678, 702, 994, 1080
SsiI CCGC 4 cut(s) 28, 40, 68, 667
SspI AATATT 2 cut(s) 629, 1002
SspMI CTAG 2 cut(s) 551, 611
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 1 cut(s) 446
StyI CCWWGG 3 cut(s) 145, 220, 375
TaaI ACNGT 2 cut(s) 460, 975
TaiI ACGT 1 cut(s) 904
TaqI TCGA 3 cut(s) 154, 207, 754
TasI AATT 6 cut(s) 250, 514, 678, 702, 994, 1080
TauI GCSGC 1 cut(s) 71
Tru1I TTAA 4 cut(s) 354, 480, 576, 681
Tru9I TTAA 4 cut(s) 354, 480, 576, 681
TscAI CASTG 2 cut(s) 275, 978
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 8 cut(s) 113, 116, 265, 395, 443, 534, 559, 1112
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 3 cut(s) 72, 96, 1122
TspRI CASTG 2 cut(s) 275, 978
Vha464I CTTAAG 1 cut(s) 353
VneI GTGCAC 1 cut(s) 271
VpaK11BI GGWCC 2 cut(s) 15, 654
XapI RAATTY 2 cut(s) 514, 994
XbaI TCTAGA 1 cut(s) 610
XceI RCATGY 2 cut(s) 245, 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 867
XmiI GTMKAC 1 cut(s) 189
XmnI GAANNNNTTC 1 cut(s) 804
XspI CTAG 2 cut(s) 551, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.