Rw4G012120

Biotin synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0015317
Physical Location & Seq
Forward (+)
28512032 .. 28517636
5605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012120.1

Sequence Viewer

Length: 1143 bp
ATGATGTTCTCCATAAGGTCCATTCTGCGCCAAGGCCTCAGACCGTCGTCGCCGTCGGCGGCATTGTACTCCTCTCTAGCTTCATCAGCAGCTGCAATTGAAGCAGAGAGAACAGTCCTACAAGGTCCTCGAAATGACTGGACTCGCGACGAGATCAGAGCCGTCTACGACTCTCCTGTTCTCGATCTCCTCTTCCATGGAGCTCAGGTTCACAGACATACACATAATTTTAGAGAAGTGCAACAGTGCACTCTCCTCTCTATCAAGACTGGCGGCTGTAGTGAGGATTGCTCCTATTGCCCTCAGTCTTCTAGATACGATACCGGACTCAAGGCACAAAAGCTTATGAATAAGGACGATGTAATGAAAGCAGCGAAAAAGGCAAAAGAGGCTGGTAGCACACGCTTTTGCATGGGTGCTGCCTGGAGGGAGACAGTTGGAAGAAAGACAAACTTTAACCAGATCCTTGAATATGTGAAAGAAATAAGGAATTTGGGAATGGAAGTGTGCTGCACCTTAGGCATGATAGAACAGCAGCAAGCGTTAAAACTTAAGGAAGCTGGCCTGACAGCTTATAATCACAATCTAGACACCTCAAGGGAATATTATCCCAATGTCATTACCACAAGGACCTATGATGACCGCTTGGAAACAATTAAGTATGTCAGGGATGCAGGAATTAGTGTGTGTTCAGGAGGAATAATAGGGCTTGGAGAAGCAGAGGAGGATCGAGTTGGTTTGTTGCATACATTAGCAACACTTCCATCTCACCCAGAAAGTGTTCCCATAAATGCGCTGATTAGTGTGAAAGGGACACCTCTTCAGGATCAAAAGCCAGTTGAAATATGGGAGATGATACGCATGATTGCCACAGCACGTATAGTCATGCCAAAAGCCATGGTCAGACTGTCAGCTGGCAGAGTTCGTTTTTCAATGCCTGAGCAGGCACTGTGCTTTCTTGCTGGGGCAAATTCAATATTTACTGGTGAAAAACTATTGACCACACCTAACAATGATTTTGATGCCGATCAAGTTATGTTCAAGGTGCTTGGACTAATTCCAAAACCTCCAAGTTTCTCCGATGAATCACCAAAGGCATCCGAGGCAGAAGCTTGTGAGGATGCTGTTTCCGTTTCAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
BATS Radical_SAM

Protein Analysis

380

Amino Acids

41.93

Weight (kDa)

6.67

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 88 - 250 1.7e-22 Radical SAM superfamily
BATS PF06968 262 - 352 6.5e-27 Biotin and Thiamin Synthesis associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43360
fragaria_vesca FvH4_4g11460 FvH4_4g11460
malus_domestica MD04G1037300.v1.1
prunus_persica Prupe.1G186700_v2.0.a1
pyrus_communis pycom04g03040
rosa_chinensis RchiOBHm_Chr4g0408131
rosa_laevigata RLG00000008670 RLG00000008673
rosa_multiflora Rmu_sc0002073.1_g000002
rosa_roxburghii Rroxscaffold_5G00352620
rosa_rugosa Rorug04G0083700
rosa_samantha Rh4AG148100 Rh4BG145300 Rh4DG141800
rosa_wichuraiana Rw4G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 576
AccI GTMKAC 1 cut(s) 165
AccII CGCG 1 cut(s) 147
AciI CCGC 3 cut(s) 59, 273, 643
AclWI GGATC 3 cut(s) 457, 735, 834
AcsI RAATTY 2 cut(s) 490, 970
AcuI CTGAAG 1 cut(s) 806
AfaI GTAC 1 cut(s) 68
AflII CTTAAG 1 cut(s) 551
AgsI TTSAA 6 cut(s) 101, 470, 842, 933, 975, 1042
AjnI CCWGG 1 cut(s) 422
AloI GAACNNNNNNTCC 2 cut(s) 192, 224
AluBI AGCT 8 cut(s) 80, 92, 203, 343, 560, 572, 914, 1112
AluI AGCT 8 cut(s) 80, 92, 203, 343, 560, 572, 914, 1112
Alw21I GWGCWC 2 cut(s) 205, 251
Alw26I GTCTC 1 cut(s) 425
Alw44I GTGCAC 1 cut(s) 247
AlwI GGATC 3 cut(s) 457, 735, 834
AlwNI CAGNNNCTG 2 cut(s) 92, 949
AoxI GGCC 2 cut(s) 34, 562
ApaLI GTGCAC 1 cut(s) 247
ApeKI GCWGC 6 cut(s) 89, 92, 371, 419, 510, 535
ApoI RAATTY 2 cut(s) 490, 970
Asp700I GAANNNNTTC 1 cut(s) 780
AspLEI GCGC 2 cut(s) 30, 796
AspS9I GGNCC 3 cut(s) 18, 125, 630
AsuHPI GGTGA 3 cut(s) 761, 998, 1080
AvaII GGWCC 3 cut(s) 18, 125, 630
AxyI CCTNAGG 1 cut(s) 517
BaeGI GKGCMC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 205
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 2 cut(s) 205, 251
BbvI GCAGC 6 cut(s) 79, 101, 383, 406, 497, 547
BccI CCATC 1 cut(s) 772
BceAI ACGGC 2 cut(s) 37, 146
BciT130I CCWGG 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 425
BfaI CTAG 3 cut(s) 77, 312, 587
BfmI CTRYAG 1 cut(s) 277
BfrI CTTAAG 1 cut(s) 551
BisI GCNGC 8 cut(s) 60, 90, 93, 274, 372, 420, 511, 536
BlsI GCNGC 8 cut(s) 61, 91, 94, 275, 373, 421, 512, 537
Bme1390I CCNGG 1 cut(s) 424
Bme18I GGWCC 3 cut(s) 18, 125, 630
BmgT120I GGNCC 3 cut(s) 18, 125, 630
BmrFI CCNGG 1 cut(s) 424
BmsI GCATC 4 cut(s) 661, 1012, 1106, 1111
BoxI GACNNNNGTC 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 300
BplI GAGNNNNNCTC 2 cut(s) 275, 307
BpmI CTGGAG 1 cut(s) 445
Bpu10I CCTNAGC 2 cut(s) 204, 939
BpuEI CTTGAG 2 cut(s) 314, 580
BsaAI YACGTR 1 cut(s) 878
BsaBI GATNNNNATC 1 cut(s) 1026
BsaJI CCNNGG 4 cut(s) 31, 196, 897, 1101
BsaWI WCCGGW 1 cut(s) 323
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bse1I ACTGG 4 cut(s) 143, 274, 836, 988
Bse21I CCTNAGG 1 cut(s) 517
Bse8I GATNNNNATC 1 cut(s) 1026
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 4 cut(s) 31, 196, 897, 1101
BseGI GGATG 3 cut(s) 676, 1097, 1126
BseJI GATNNNNATC 1 cut(s) 1026
BseMII CTCAG 4 cut(s) 52, 218, 317, 930
BseNI ACTGG 4 cut(s) 143, 274, 836, 988
BseRI GAGGAG 4 cut(s) 61, 179, 245, 737
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 6 cut(s) 79, 101, 383, 406, 497, 547
BseYI CCCAGC 1 cut(s) 962
BsgI GTGCAG 1 cut(s) 496
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 2 cut(s) 36, 564
BsiHKAI GWGCWC 2 cut(s) 205, 251
BsiSI CCGG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 826
BsmAI GTCTC 1 cut(s) 425
BsmFI GGGAC 1 cut(s) 826
BsnI GGCC 2 cut(s) 36, 564
Bsp1286I GDGCHC 2 cut(s) 205, 251
Bsp143I GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
Bsp19I CCATGG 2 cut(s) 196, 897
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 3 cut(s) 59, 273, 643
BspANI GGCC 2 cut(s) 36, 564
BspCNI CTCAG 4 cut(s) 51, 217, 316, 931
BspFNI CGCG 1 cut(s) 147
BspPI GGATC 3 cut(s) 457, 735, 834
BspTI CTTAAG 1 cut(s) 551
BsrI ACTGG 4 cut(s) 143, 274, 836, 988
BssECI CCNNGG 4 cut(s) 31, 196, 897, 1101
BssMI GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
BssT1I CCWWGG 3 cut(s) 31, 196, 897
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 6 cut(s) 45, 115, 246, 436, 909, 951
Bst6I CTCTTC 2 cut(s) 197, 825
BstAFI CTTAAG 1 cut(s) 551
BstBAI YACGTR 1 cut(s) 878
BstC8I GCNNGC 4 cut(s) 540, 562, 916, 945
BstDEI CTNAG 5 cut(s) 38, 204, 303, 517, 939
BstDSI CCRYGG 2 cut(s) 196, 897
BstF5I GGATG 3 cut(s) 676, 1097, 1126
BstFNI CGCG 1 cut(s) 147
BstHHI GCGC 2 cut(s) 30, 796
BstKTI GATC 6 cut(s) 156, 187, 465, 730, 829, 1030
BstMAI GTCTC 1 cut(s) 425
BstMBI GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
BstMWI GCNNNNNNNGC 7 cut(s) 86, 101, 297, 380, 389, 519, 1103
BstNI CCWGG 1 cut(s) 424
BstPAI GACNNNNGTC 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 422
BstSFI CTRYAG 1 cut(s) 277
BstSLI GKGCMC 1 cut(s) 251
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 6 cut(s) 79, 101, 383, 406, 497, 547
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 462
BstYI RGATCY 1 cut(s) 462
Bsu36I CCTNAGG 1 cut(s) 517
BsuRI GGCC 2 cut(s) 36, 564
BtgI CCRYGG 2 cut(s) 196, 897
BtsCI GGATG 3 cut(s) 676, 1097, 1126
BtsIMutI CAGTG 2 cut(s) 251, 947
BtuMI TCGCGA 1 cut(s) 147
Cac8I GCNNGC 4 cut(s) 540, 562, 916, 945
CaiI CAGNNNCTG 2 cut(s) 92, 949
CfoI GCGC 2 cut(s) 30, 796
Cfr13I GGNCC 3 cut(s) 18, 125, 630
Csp6I GTAC 1 cut(s) 67
CviAII CATG 6 cut(s) 197, 412, 523, 862, 886, 898
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 5 cut(s) 38, 204, 303, 517, 939
DpnI GATC 6 cut(s) 155, 186, 464, 729, 828, 1029
DpnII GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
Eam1104I CTCTTC 2 cut(s) 197, 825
EarI CTCTTC 2 cut(s) 197, 825
Ecl136II GAGCTC 1 cut(s) 203
Eco130I CCWWGG 3 cut(s) 31, 196, 897
Eco147I AGGCCT 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 205
Eco47I GGWCC 3 cut(s) 18, 125, 630
Eco53kI GAGCTC 1 cut(s) 203
Eco57I CTGAAG 1 cut(s) 806
Eco81I CCTNAGG 1 cut(s) 517
EcoICRI GAGCTC 1 cut(s) 203
EcoO109I RGGNCCY 2 cut(s) 125, 630
EcoRII CCWGG 1 cut(s) 422
EcoT14I CCWWGG 3 cut(s) 31, 196, 897
EcoT38I GRGCYC 1 cut(s) 205
ErhI CCWWGG 3 cut(s) 31, 196, 897
FaeI CATG 6 cut(s) 200, 415, 526, 865, 889, 901
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 1 cut(s) 826
FatI CATG 6 cut(s) 196, 411, 522, 861, 885, 897
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 8 cut(s) 60, 90, 93, 274, 372, 420, 511, 536
FokI GGATG 3 cut(s) 683, 1084, 1133
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 8 cut(s) 60, 90, 93, 274, 372, 420, 511, 536
FspBI CTAG 3 cut(s) 77, 312, 587
GlaI GCGC 2 cut(s) 29, 795
GluI GCNGC 8 cut(s) 60, 90, 93, 274, 372, 420, 511, 536
GsaI CCCAGC 1 cut(s) 966
GsuI CTGGAG 1 cut(s) 445
HaeIII GGCC 2 cut(s) 36, 564
HapII CCGG 1 cut(s) 324
HhaI GCGC 2 cut(s) 30, 796
Hin1II CATG 6 cut(s) 200, 415, 526, 865, 889, 901
Hin6I GCGC 2 cut(s) 28, 794
HinP1I GCGC 2 cut(s) 28, 794
HindIII AAGCTT 2 cut(s) 341, 1110
HinfI GANTC 4 cut(s) 142, 170, 327, 1085
HpaII CCGG 1 cut(s) 324
HphI GGTGA 3 cut(s) 761, 998, 1080
Hpy166II GTNNAC 3 cut(s) 166, 211, 249
Hpy188I TCNGA 5 cut(s) 41, 158, 905, 1081, 1102
Hpy188III TCNNGA 7 cut(s) 146, 182, 265, 312, 587, 693, 824
Hpy8I GTNNAC 3 cut(s) 166, 211, 249
Hpy99I CGWCG 4 cut(s) 49, 52, 58, 152
HpyCH4III ACNGT 6 cut(s) 45, 115, 246, 436, 909, 951
HpyCH4IV ACGT 1 cut(s) 877
HpyCH4V TGCA 7 cut(s) 95, 241, 249, 411, 513, 674, 745
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 101, 297, 380, 389, 519, 1103
HpyF3I CTNAG 5 cut(s) 38, 204, 303, 517, 939
HpySE526I ACGT 1 cut(s) 877
Hsp92II CATG 6 cut(s) 200, 415, 526, 865, 889, 901
HspAI GCGC 2 cut(s) 28, 794
Kzo9I GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
LmnI GCTCC 2 cut(s) 200, 296
Lsp1109I GCAGC 6 cut(s) 79, 101, 383, 406, 497, 547
LweI GCATC 4 cut(s) 661, 1012, 1106, 1111
MaeI CTAG 3 cut(s) 77, 312, 587
MaeII ACGT 1 cut(s) 877
MalI GATC 6 cut(s) 155, 186, 464, 729, 828, 1029
MboI GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
MboII GAAGA 4 cut(s) 184, 300, 453, 812
MfeI CAATTG 1 cut(s) 96
MflI RGATCY 1 cut(s) 462
MhlI GDGCHC 2 cut(s) 205, 251
MluCI AATT 7 cut(s) 96, 226, 490, 654, 678, 970, 1056
MlyI GAGTC 3 cut(s) 136, 164, 321
MmeI TCCRAC 1 cut(s) 418
MroXI GAANNNNTTC 1 cut(s) 780
MseI TTAA 4 cut(s) 456, 545, 552, 657
MspA1I CMGCKG 2 cut(s) 92, 914
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 1 cut(s) 324
MspR9I CCNGG 1 cut(s) 424
MunI CAATTG 1 cut(s) 96
MvaI CCWGG 1 cut(s) 424
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 7 cut(s) 86, 101, 297, 380, 389, 519, 1103
NcoI CCATGG 2 cut(s) 196, 897
NdeII GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
NlaIII CATG 6 cut(s) 200, 415, 526, 865, 889, 901
NruI TCGCGA 1 cut(s) 147
PceI AGGCCT 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 780
PfeI GAWTC 1 cut(s) 1085
PkrI GCNGC 8 cut(s) 61, 91, 94, 275, 373, 421, 512, 537
PleI GAGTC 3 cut(s) 136, 164, 321
PpsI GAGTC 3 cut(s) 136, 164, 321
Ppu21I YACGTR 1 cut(s) 878
PpuMI RGGWCCY 2 cut(s) 125, 630
PshAI GACNNNNGTC 1 cut(s) 46
PsiI TTATAA 1 cut(s) 576
Psp124BI GAGCTC 1 cut(s) 205
Psp5II RGGWCCY 2 cut(s) 125, 630
Psp6I CCWGG 1 cut(s) 422
PspFI CCCAGC 1 cut(s) 962
PspGI CCWGG 1 cut(s) 422
PspPI GGNCC 3 cut(s) 18, 125, 630
PspPPI RGGWCCY 2 cut(s) 125, 630
PstNI CAGNNNCTG 2 cut(s) 92, 949
PsuI RGATCY 1 cut(s) 462
PvuII CAGCTG 2 cut(s) 92, 914
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
SacI GAGCTC 1 cut(s) 205
SaqAI TTAA 4 cut(s) 456, 545, 552, 657
SatI GCNGC 8 cut(s) 60, 90, 93, 274, 372, 420, 511, 536
Sau3AI GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
Sau96I GGNCC 3 cut(s) 18, 125, 630
SchI GAGTC 3 cut(s) 136, 164, 321
ScrFI CCNGG 1 cut(s) 424
SduI GDGCHC 2 cut(s) 205, 251
SfaNI GCATC 4 cut(s) 661, 1012, 1106, 1111
SfcI CTRYAG 1 cut(s) 277
SinI GGWCC 3 cut(s) 18, 125, 630
SmlI CTYRAG 3 cut(s) 329, 551, 595
SmoI CTYRAG 3 cut(s) 329, 551, 595
Sse9I AATT 7 cut(s) 96, 226, 490, 654, 678, 970, 1056
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 3 cut(s) 59, 273, 643
SspI AATATT 2 cut(s) 605, 978
SspMI CTAG 3 cut(s) 77, 312, 587
SstI GAGCTC 1 cut(s) 205
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 422
StyI CCWWGG 3 cut(s) 31, 196, 897
TaaI ACNGT 6 cut(s) 45, 115, 246, 436, 909, 951
TaiI ACGT 1 cut(s) 880
TaqI TCGA 3 cut(s) 130, 183, 730
TasI AATT 7 cut(s) 96, 226, 490, 654, 678, 970, 1056
TatI WGTACW 1 cut(s) 66
TauI GCSGC 2 cut(s) 62, 276
TfiI GAWTC 1 cut(s) 1085
Tru1I TTAA 4 cut(s) 456, 545, 552, 657
Tru9I TTAA 4 cut(s) 456, 545, 552, 657
TscAI CASTG 2 cut(s) 251, 954
TseI GCWGC 6 cut(s) 89, 92, 371, 419, 510, 535
TspDTI ATGAA 4 cut(s) 72, 362, 380, 1098
TspGWI ACGGA 1 cut(s) 1120
TspRI CASTG 2 cut(s) 251, 954
Vha464I CTTAAG 1 cut(s) 551
VneI GTGCAC 1 cut(s) 247
VpaK11BI GGWCC 3 cut(s) 18, 125, 630
XapI RAATTY 2 cut(s) 490, 970
XbaI TCTAGA 2 cut(s) 311, 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 843
XmiI GTMKAC 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 780
XspI CTAG 3 cut(s) 77, 312, 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.