pycom04g03040

Biotin synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015317
Physical Location & Seq
Reverse (-)
2814793 .. 2819200
4408 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g03040.6

Sequence Viewer

Length: 1146 bp
ATGTTTTCCATTAGGTCCATAGTGCGTGGGCAGCTGAGACCTTCGGCGGTTGTGTTGCATTCTTCATTTTACTCTTCCTTATCGTCTGCAGCTGCAGTTCAAGCTGAGAGAACAATCGCACAAGGCCCCAGAAATGACTGGACCCGCGACGAGATCAAGGGCGTCTACGGCTCTCCTGTTCTCGATCTCCTTTTTCACGGAGCTCAAGTTCATAGGCACGCCCATAATTTTAGGGAAGTGCAGCAGTGTACTCTCCTCTCTATAAAGACGGGTGGATGTAGTGAGGATTGCTCATATTGTCCTCAATCCTCCCGGTATAACACAGGACTCAAGGCCCAAAAGCTTATGACAAAGGATGCTGTCATGGAAGCAGCACAGAGGGCAAAAGAGGCTGGAAGCACACGGTTTTGCATGGGTGCTGCCTGGAGGGATACAATAGGAAGAAAGACAAACTTTAACCAGATCCTTGAATATGTAAAAGACATAAGGGGTATGGGAATGGAAGTCTGCTGCACCTTAGGCATGATAGAAAAGCAACAAGCTTTGAAACTCAAAGAAGCAGGCCTGACAGCTTATAATCACAATCTTGACACCTCAAGGGAATATTACCCGAATATCATTACCACAAGGACCTATGATGAACGCTTGGAAACCATTAAGCATGTCCGGGATGCGGGAATTAATGTGTGTTCAGGAGGAATAATAGGACTTGGAGAAGCAGAGGAGGATCGAGTTGGTTTGCTGCATACATTAGCAACACTCCCAACTCACCCAGAAAGCGTCCCCATCAATGCACTGGTTTCAGTGAAAGGAACACCTCTTCAGGATCAAAAGCCGGTTGAAATCTGGGAGATGATACGGATGATTGCCACAGCACGTATAGTTATGCCAAATGCAATGGTCAGATTGTCAGCTGGCAGAGTTAGGTTCTCTATGCCCGAGCAGGCATTGTGTTTTCTTGCCGGGGCAAATTCAATATTCACTGGGGAGAAGCTCTTGACTACACCGAACAACGATTTTGATGCTGATCAACTCATGTTCAAGATGCTTGGTTTGATTCCAAAAGCTCCTAGTTTCTCTGATGAACCAGCAAACGCGGCGTGTGAGGTAGATGCTTGTGAGGAAGCTGTTTCCAGTTCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
BATS Radical_SAM

Protein Analysis

382

Amino Acids

41.87

Weight (kDa)

6.76

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43360
fragaria_vesca FvH4_4g11460 FvH4_4g11460
malus_domestica MD04G1037300.v1.1
prunus_persica Prupe.1G186700_v2.0.a1
pyrus_communis pycom04g03040
rosa_chinensis RchiOBHm_Chr4g0408131
rosa_laevigata RLG00000008670 RLG00000008673
rosa_multiflora Rmu_sc0002073.1_g000002
rosa_roxburghii Rroxscaffold_5G00352620
rosa_rugosa Rorug04G0083700
rosa_samantha Rh4AG148100 Rh4BG145300 Rh4DG141800
rosa_wichuraiana Rw4G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 576
AccI GTMKAC 1 cut(s) 165
AccII CGCG 2 cut(s) 147, 1097
AciI CCGC 4 cut(s) 47, 145, 674, 1097
AclWI GGATC 3 cut(s) 457, 735, 834
AcsI RAATTY 1 cut(s) 970
AcuI CTGAAG 1 cut(s) 806
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 1 cut(s) 250
AfiI CCNNNNNNNGG 1 cut(s) 673
AgsI TTSAA 6 cut(s) 101, 470, 547, 842, 975, 1042
AjnI CCWGG 1 cut(s) 422
AloI GAACNNNNNNTCC 2 cut(s) 192, 224
Alw21I GWGCWC 1 cut(s) 205
Alw26I GTCTC 1 cut(s) 31
AlwI GGATC 3 cut(s) 457, 735, 834
Ama87I CYCGRG 1 cut(s) 938
AoxI GGCC 3 cut(s) 124, 333, 562
ApeKI GCWGC 8 cut(s) 31, 89, 92, 241, 371, 419, 510, 742
ApoI RAATTY 1 cut(s) 970
AseI ATTAAT 1 cut(s) 681
AspS9I GGNCC 5 cut(s) 15, 125, 141, 334, 630
AsuC2I CCSGG 3 cut(s) 313, 668, 964
AsuHPI GGTGA 1 cut(s) 761
AvaI CYCGRG 1 cut(s) 938
AvaII GGWCC 3 cut(s) 15, 141, 630
AxyI CCTNAGG 1 cut(s) 517
BanII GRGCYC 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 205
BbvI GCAGC 8 cut(s) 43, 79, 101, 253, 383, 406, 497, 729
BccI CCATC 1 cut(s) 794
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 1004, 1038
BciT130I CCWGG 1 cut(s) 424
BciVI GTATCC 1 cut(s) 424
BclI TGATCA 1 cut(s) 1027
BcnI CCSGG 3 cut(s) 313, 668, 964
BcoDI GTCTC 1 cut(s) 31
BfaI CTAG 1 cut(s) 1071
BfmI CTRYAG 2 cut(s) 87, 93
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 9 cut(s) 32, 90, 93, 242, 372, 420, 511, 743, 1098
BlsI GCNGC 9 cut(s) 33, 91, 94, 243, 373, 421, 512, 744, 1099
Bme1390I CCNGG 4 cut(s) 313, 424, 668, 964
Bme18I GGWCC 3 cut(s) 15, 141, 630
BmeT110I CYCGRG 1 cut(s) 938
BmgT120I GGNCC 5 cut(s) 15, 125, 141, 334, 630
BmiI GGNNCC 2 cut(s) 127, 143
BmrFI CCNGG 4 cut(s) 313, 424, 668, 964
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 5 cut(s) 346, 661, 1012, 1035, 1102
BmuI ACTGGG 1 cut(s) 993
BplI GAGNNNNNCTC 2 cut(s) 275, 307
BpmI CTGGAG 1 cut(s) 445
BpuEI CTTGAG 3 cut(s) 189, 314, 580
BpuMI CCSGG 3 cut(s) 313, 668, 964
BsaAI YACGTR 1 cut(s) 878
BsaBI GATNNNNATC 1 cut(s) 1026
BsaHI GRCGYC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 963
BsaXI ACNNNNNCTCC 2 cut(s) 192, 222
Bsc4I CCNNNNNNNGG 1 cut(s) 673
Bse118I RCCGGY 1 cut(s) 835
Bse1I ACTGG 4 cut(s) 143, 801, 988, 1134
Bse21I CCTNAGG 1 cut(s) 517
Bse3DI GCAATG 1 cut(s) 903
Bse8I GATNNNNATC 1 cut(s) 1026
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 963
BseGI GGATG 4 cut(s) 281, 361, 676, 867
BseJI GATNNNNATC 1 cut(s) 1026
BseLI CCNNNNNNNGG 1 cut(s) 673
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 2 cut(s) 26, 96
BseNI ACTGG 4 cut(s) 143, 801, 988, 1134
BseRI GAGGAG 2 cut(s) 245, 737
BseXI GCAGC 8 cut(s) 43, 79, 101, 253, 383, 406, 497, 729
BsgI GTGCAG 2 cut(s) 260, 496
Bsh1236I CGCG 2 cut(s) 147, 1097
BshFI GGCC 3 cut(s) 126, 335, 564
BsiHKAI GWGCWC 1 cut(s) 205
BsiHKCI CYCGRG 1 cut(s) 938
BsiSI CCGG 4 cut(s) 313, 667, 836, 963
BslFI GGGAC 1 cut(s) 767
BslI CCNNNNNNNGG 1 cut(s) 673
BsmAI GTCTC 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 767
BsmI GAATGC 1 cut(s) 58
BsnI GGCC 3 cut(s) 126, 335, 564
Bso31I GGTCTC 1 cut(s) 31
BsoBI CYCGRG 1 cut(s) 938
Bsp1286I GDGCHC 1 cut(s) 205
Bsp143I GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
BspACI CCGC 4 cut(s) 47, 145, 674, 1097
BspANI GGCC 3 cut(s) 126, 335, 564
BspCNI CTCAG 2 cut(s) 27, 97
BspFNI CGCG 2 cut(s) 147, 1097
BspLI GGNNCC 2 cut(s) 127, 143
BspMAI CTGCAG 2 cut(s) 91, 97
BspPI GGATC 3 cut(s) 457, 735, 834
BspTNI GGTCTC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 835
BsrI ACTGG 4 cut(s) 143, 801, 988, 1134
BssAI RCCGGY 1 cut(s) 835
BssECI CCNNGG 1 cut(s) 963
BssMI GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
BssNI GRCGYC 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 1 cut(s) 405
Bst6I CTCTTC 2 cut(s) 79, 825
BstACI GRCGYC 1 cut(s) 162
BstBAI YACGTR 1 cut(s) 878
BstC8I GCNNGC 4 cut(s) 219, 562, 916, 945
BstDEI CTNAG 3 cut(s) 35, 105, 517
BstF5I GGATG 4 cut(s) 281, 361, 676, 867
BstFNI CGCG 2 cut(s) 147, 1097
BstKTI GATC 6 cut(s) 156, 187, 465, 730, 829, 1030
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
BstMWI GCNNNNNNNGC 7 cut(s) 31, 101, 168, 380, 389, 519, 1097
BstNI CCWGG 1 cut(s) 424
BstNSI RCATGY 1 cut(s) 665
BstSCI CCNGG 4 cut(s) 311, 422, 666, 962
BstSFI CTRYAG 2 cut(s) 87, 93
BstUI CGCG 2 cut(s) 147, 1097
BstV1I GCAGC 8 cut(s) 43, 79, 101, 253, 383, 406, 497, 729
BstX2I RGATCY 1 cut(s) 462
BstYI RGATCY 1 cut(s) 462
Bsu36I CCTNAGG 1 cut(s) 517
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 3 cut(s) 126, 335, 564
BtsCI GGATG 4 cut(s) 281, 361, 676, 867
BtsI GCAGTG 1 cut(s) 251
BtsIMutI CAGTG 4 cut(s) 251, 794, 810, 981
Cac8I GCNNGC 4 cut(s) 219, 562, 916, 945
Cfr10I RCCGGY 1 cut(s) 835
Cfr13I GGNCC 5 cut(s) 15, 125, 141, 334, 630
CseI GACGC 2 cut(s) 151, 769
Csp6I GTAC 1 cut(s) 249
CviAII CATG 5 cut(s) 364, 412, 523, 662, 1036
CviQI GTAC 1 cut(s) 249
DdeI CTNAG 3 cut(s) 35, 105, 517
DpnI GATC 6 cut(s) 155, 186, 464, 729, 828, 1029
DpnII GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
Eam1104I CTCTTC 2 cut(s) 79, 825
EarI CTCTTC 2 cut(s) 79, 825
Ecl136II GAGCTC 1 cut(s) 203
Eco147I AGGCCT 1 cut(s) 564
Eco24I GRGCYC 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 31
Eco47I GGWCC 3 cut(s) 15, 141, 630
Eco53kI GAGCTC 1 cut(s) 203
Eco57I CTGAAG 1 cut(s) 806
Eco81I CCTNAGG 1 cut(s) 517
Eco88I CYCGRG 1 cut(s) 938
EcoICRI GAGCTC 1 cut(s) 203
EcoO109I RGGNCCY 2 cut(s) 125, 630
EcoRII CCWGG 1 cut(s) 422
EcoT38I GRGCYC 1 cut(s) 205
FaeI CATG 5 cut(s) 367, 415, 526, 665, 1039
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 1 cut(s) 767
FatI CATG 5 cut(s) 363, 411, 522, 661, 1035
FauI CCCGC 2 cut(s) 152, 667
FbaI TGATCA 1 cut(s) 1027
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 9 cut(s) 32, 90, 93, 242, 372, 420, 511, 743, 1098
FokI GGATG 4 cut(s) 288, 368, 683, 874
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 9 cut(s) 32, 90, 93, 242, 372, 420, 511, 743, 1098
FspBI CTAG 1 cut(s) 1071
GluI GCNGC 9 cut(s) 32, 90, 93, 242, 372, 420, 511, 743, 1098
GsuI CTGGAG 1 cut(s) 445
HaeIII GGCC 3 cut(s) 126, 335, 564
HapII CCGG 4 cut(s) 313, 667, 836, 963
HgaI GACGC 2 cut(s) 151, 769
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 5 cut(s) 367, 415, 526, 665, 1039
HindIII AAGCTT 2 cut(s) 341, 540
HinfI GANTC 2 cut(s) 327, 1057
HpaII CCGG 4 cut(s) 313, 667, 836, 963
HphI GGTGA 1 cut(s) 761
Hpy166II GTNNAC 2 cut(s) 166, 249
Hpy188I TCNGA 2 cut(s) 905, 1081
Hpy188III TCNNGA 6 cut(s) 182, 587, 693, 824, 997, 1042
Hpy8I GTNNAC 2 cut(s) 166, 249
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 1 cut(s) 51
HpyCH4III ACNGT 1 cut(s) 405
HpyCH4IV ACGT 1 cut(s) 877
HpyCH4V TGCA 9 cut(s) 58, 89, 95, 241, 411, 513, 745, 794, 896
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 101, 168, 380, 389, 519, 1097
HpyF3I CTNAG 3 cut(s) 35, 105, 517
HpySE526I ACGT 1 cut(s) 877
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 5 cut(s) 367, 415, 526, 665, 1039
Ksp22I TGATCA 1 cut(s) 1027
Kzo9I GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
LmnI GCTCC 2 cut(s) 200, 1072
Lsp1109I GCAGC 8 cut(s) 43, 79, 101, 253, 383, 406, 497, 729
LweI GCATC 5 cut(s) 346, 661, 1012, 1035, 1102
MaeI CTAG 1 cut(s) 1071
MaeII ACGT 1 cut(s) 877
MalI GATC 6 cut(s) 155, 186, 464, 729, 828, 1029
MboI GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
MboII GAAGA 4 cut(s) 54, 66, 453, 812
MflI RGATCY 1 cut(s) 462
MhlI GDGCHC 1 cut(s) 205
MluCI AATT 3 cut(s) 226, 678, 970
MlyI GAGTC 1 cut(s) 321
MseI TTAA 3 cut(s) 456, 657, 681
MspA1I CMGCKG 3 cut(s) 34, 92, 914
MspI CCGG 4 cut(s) 313, 667, 836, 963
MspR9I CCNGG 4 cut(s) 313, 424, 668, 964
Mva1269I GAATGC 1 cut(s) 58
MvaI CCWGG 1 cut(s) 424
MvnI CGCG 2 cut(s) 147, 1097
MwoI GCNNNNNNNGC 7 cut(s) 31, 101, 168, 380, 389, 519, 1097
NciI CCSGG 3 cut(s) 313, 668, 964
NdeII GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
NlaIII CATG 5 cut(s) 367, 415, 526, 665, 1039
NlaIV GGNNCC 2 cut(s) 127, 143
NspI RCATGY 1 cut(s) 665
PceI AGGCCT 1 cut(s) 564
PctI GAATGC 1 cut(s) 58
PfeI GAWTC 1 cut(s) 1057
PfoI TCCNGGA 1 cut(s) 666
PkrI GCNGC 9 cut(s) 33, 91, 94, 243, 373, 421, 512, 744, 1099
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
Ppu21I YACGTR 1 cut(s) 878
PpuMI RGGWCCY 1 cut(s) 630
PshBI ATTAAT 1 cut(s) 681
PsiI TTATAA 1 cut(s) 576
Psp124BI GAGCTC 1 cut(s) 205
Psp5II RGGWCCY 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 2 cut(s) 127, 143
PspPI GGNCC 5 cut(s) 15, 125, 141, 334, 630
PspPPI RGGWCCY 1 cut(s) 630
PstI CTGCAG 2 cut(s) 91, 97
PsuI RGATCY 1 cut(s) 462
PvuII CAGCTG 3 cut(s) 34, 92, 914
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
SacI GAGCTC 1 cut(s) 205
SaqAI TTAA 3 cut(s) 456, 657, 681
SatI GCNGC 9 cut(s) 32, 90, 93, 242, 372, 420, 511, 743, 1098
Sau3AI GATC 6 cut(s) 153, 184, 462, 727, 826, 1027
Sau96I GGNCC 5 cut(s) 15, 125, 141, 334, 630
SchI GAGTC 1 cut(s) 321
ScrFI CCNGG 4 cut(s) 313, 424, 668, 964
SduI GDGCHC 1 cut(s) 205
SfaNI GCATC 5 cut(s) 346, 661, 1012, 1035, 1102
SfcI CTRYAG 2 cut(s) 87, 93
SinI GGWCC 3 cut(s) 15, 141, 630
SmlI CTYRAG 3 cut(s) 204, 329, 595
SmoI CTYRAG 3 cut(s) 204, 329, 595
Sse9I AATT 3 cut(s) 226, 678, 970
SseBI AGGCCT 1 cut(s) 564
SsiI CCGC 4 cut(s) 47, 145, 674, 1097
SspI AATATT 2 cut(s) 605, 978
SspMI CTAG 1 cut(s) 1071
SstI GAGCTC 1 cut(s) 205
StuI AGGCCT 1 cut(s) 564
StyD4I CCNGG 4 cut(s) 311, 422, 666, 962
TaaI ACNGT 1 cut(s) 405
TaiI ACGT 1 cut(s) 880
TaqI TCGA 2 cut(s) 183, 730
TasI AATT 3 cut(s) 226, 678, 970
TatI WGTACW 1 cut(s) 248
TauI GCSGC 1 cut(s) 1100
TfiI GAWTC 1 cut(s) 1057
Tru1I TTAA 3 cut(s) 456, 657, 681
Tru9I TTAA 3 cut(s) 456, 657, 681
TscAI CASTG 4 cut(s) 251, 801, 810, 988
TseI GCWGC 8 cut(s) 31, 89, 92, 241, 371, 419, 510, 742
TspDTI ATGAA 4 cut(s) 54, 200, 654, 1098
TspGWI ACGGA 2 cut(s) 213, 874
TspRI CASTG 4 cut(s) 251, 801, 810, 988
VpaK11BI GGWCC 3 cut(s) 15, 141, 630
VspI ATTAAT 1 cut(s) 681
XapI RAATTY 1 cut(s) 970
XceI RCATGY 1 cut(s) 665
XcmI CCANNNNNNNNNTGG 1 cut(s) 793
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 1 cut(s) 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.