Rh4AG148100

Biotin synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0015317
Physical Location & Seq
Forward (+)
37468752 .. 37474596
5845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG148100.1

Sequence Viewer

Length: 1155 bp
ATGATGTTCTCCGTTAGGTACATTCTGCGCCAAGGCCTCAGACCGCCGTCGTCGTCGGCGTCGGCGTCGGCGGCATTTTACTCCTCTCTATCTTCATCATCAGCTGCAATTGAAGCAGAGAGAACAGTCCTACAAGGTCCTCGAAATGACTGGACTCGCGACGAGATCAGAGCCGTCTACGACTCTCCTGTTCTCGATCTCCTCTTCCATGGAGCTCAGGTTCACAGACATGCACATAATTTTAGAGAAGTGCAACAGTGCACTCTCCTCTCTATCAAGACTGGCGGCTGTAGTGAGGATTGCTCCTATTGCCCTCAGTCCTCTAGATACGATACCGGACTCAAGGCCCAAAAGCTTATGAATAAGGACGATGTAATGAAAGCAGCGAAAAAGGCAAAAGAGGCTGGTAGCACACGCTTTTGCATGGGTGCTGCCTGGAGGGAGACAGTAGGAAGAAAGACAAACTTTAACCAGATCCTTGAATATGTGAAAGAAATAAGGAATTTGGGAATGGAAGTGTGCTGCACCTTAGGCATGATAGAACAGCAGCAAGCGTTAAAACTTAAGGAAGCTGGCCTGACAGCTTATAATCACAATCTAGACACCTCAAGGGAATATTATCCCAATGTCATTACCACAAGGACCTATGATGACCGCTTGGAAACAATTAAGTATGTCAGGGATGCAGGAATTAGTGTGTGTTCAGGAGGAATAATAGGGCTTGGAGAAGCAGAGGAGGATCGAGTTGGTTTGTTGCATACATTAGCAACACTTCCATCTCACCCAGAAAGTGTTCCCATAAACGCGCTGATTAGTGTGAAAGGGACACCTCTTCAGGATCAAAAGCCAGTTGAAATATGGGAGATGATACGCATGATTGCCACAGCACGGATAGTCATGCCAAAAGCCATGGTCAGACTGTCAGCTGGCAGAGTTCGTTTTTCAATGCCTGAGCAGGCACTGTGCTTTCTTGCTGGGGCAAATTCAATATTTACTGGTGAAAAACTATTGACCACACCTAACAATGATTTTGATGCCGATCAAGTTATGTTCAAGGTGCTTGGACTAATTCCAAAACCTCCAAGTTTCTCCGATGAATCAGCAAAGGCATCCGAGGCAGAAGCTTGTGAGGATGCTGTTTCCGTTTCAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
BATS Radical_SAM

Protein Analysis

384

Amino Acids

42.32

Weight (kDa)

6.67

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 92 - 254 1.7e-22 Radical SAM superfamily
BATS PF06968 266 - 356 6.6e-27 Biotin and Thiamin Synthesis associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43360
fragaria_vesca FvH4_4g11460 FvH4_4g11460
malus_domestica MD04G1037300.v1.1
prunus_persica Prupe.1G186700_v2.0.a1
pyrus_communis pycom04g03040
rosa_chinensis RchiOBHm_Chr4g0408131
rosa_laevigata RLG00000008670 RLG00000008673
rosa_multiflora Rmu_sc0002073.1_g000002
rosa_roxburghii Rroxscaffold_5G00352620
rosa_rugosa Rorug04G0083700
rosa_samantha Rh4AG148100 Rh4BG145300 Rh4DG141800
rosa_wichuraiana Rw4G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 588
AccI GTMKAC 1 cut(s) 177
AccII CGCG 2 cut(s) 159, 806
AciI CCGC 4 cut(s) 44, 71, 285, 655
AclWI GGATC 3 cut(s) 469, 747, 846
AcsI RAATTY 2 cut(s) 502, 982
AcuI CTGAAG 1 cut(s) 818
AcyI GRCGYC 2 cut(s) 59, 65
AfaI GTAC 1 cut(s) 20
AfiI CCNNNNNNNGG 1 cut(s) 888
AflII CTTAAG 1 cut(s) 563
AgsI TTSAA 6 cut(s) 113, 482, 854, 945, 987, 1054
AjnI CCWGG 1 cut(s) 434
AloI GAACNNNNNNTCC 2 cut(s) 204, 236
AluBI AGCT 7 cut(s) 104, 215, 355, 572, 584, 926, 1124
AluI AGCT 7 cut(s) 104, 215, 355, 572, 584, 926, 1124
Alw21I GWGCWC 2 cut(s) 217, 263
Alw26I GTCTC 1 cut(s) 437
Alw44I GTGCAC 1 cut(s) 259
AlwI GGATC 3 cut(s) 469, 747, 846
AlwNI CAGNNNCTG 1 cut(s) 961
AoxI GGCC 3 cut(s) 34, 345, 574
ApaLI GTGCAC 1 cut(s) 259
ApeKI GCWGC 5 cut(s) 104, 383, 431, 522, 547
ApoI RAATTY 2 cut(s) 502, 982
Asp700I GAANNNNTTC 1 cut(s) 792
AspLEI GCGC 2 cut(s) 30, 808
AspS9I GGNCC 3 cut(s) 137, 346, 642
AsuHPI GGTGA 2 cut(s) 773, 1010
AvaII GGWCC 2 cut(s) 137, 642
AxyI CCTNAGG 1 cut(s) 529
BaeGI GKGCMC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 217
Bbv12I GWGCWC 2 cut(s) 217, 263
BbvI GCAGC 5 cut(s) 91, 395, 418, 509, 559
BccI CCATC 1 cut(s) 784
BceAI ACGGC 2 cut(s) 31, 158
BciT130I CCWGG 1 cut(s) 436
BcoDI GTCTC 1 cut(s) 437
BfaI CTAG 2 cut(s) 324, 599
BfmI CTRYAG 1 cut(s) 289
BfrI CTTAAG 1 cut(s) 563
BisI GCNGC 7 cut(s) 72, 105, 286, 384, 432, 523, 548
BlsI GCNGC 7 cut(s) 73, 106, 287, 385, 433, 524, 549
Bme1390I CCNGG 1 cut(s) 436
Bme18I GGWCC 2 cut(s) 137, 642
BmgT120I GGNCC 3 cut(s) 137, 346, 642
BmrFI CCNGG 1 cut(s) 436
BmsI GCATC 4 cut(s) 673, 1024, 1118, 1123
BoxI GACNNNNGTC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpmI CTGGAG 1 cut(s) 457
Bpu10I CCTNAGC 2 cut(s) 216, 951
BpuEI CTTGAG 2 cut(s) 326, 592
BsaBI GATNNNNATC 1 cut(s) 1038
BsaHI GRCGYC 2 cut(s) 59, 65
BsaJI CCNNGG 4 cut(s) 31, 208, 909, 1113
BsaWI WCCGGW 1 cut(s) 335
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 888
Bse1I ACTGG 4 cut(s) 155, 286, 848, 1000
Bse21I CCTNAGG 1 cut(s) 529
Bse8I GATNNNNATC 1 cut(s) 1038
BseBI CCWGG 1 cut(s) 436
BseDI CCNNGG 4 cut(s) 31, 208, 909, 1113
BseGI GGATG 3 cut(s) 688, 1109, 1138
BseJI GATNNNNATC 1 cut(s) 1038
BseLI CCNNNNNNNGG 1 cut(s) 888
BseMII CTCAG 4 cut(s) 52, 230, 329, 942
BseNI ACTGG 4 cut(s) 155, 286, 848, 1000
BseRI GAGGAG 4 cut(s) 73, 191, 257, 749
BseSI GKGCMC 1 cut(s) 263
BseXI GCAGC 5 cut(s) 91, 395, 418, 509, 559
BseYI CCCAGC 1 cut(s) 974
BsgI GTGCAG 1 cut(s) 508
Bsh1236I CGCG 2 cut(s) 159, 806
BshFI GGCC 3 cut(s) 36, 347, 576
BsiHKAI GWGCWC 2 cut(s) 217, 263
BsiSI CCGG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 838
BslI CCNNNNNNNGG 1 cut(s) 888
BsmAI GTCTC 1 cut(s) 437
BsmFI GGGAC 1 cut(s) 838
BsnI GGCC 3 cut(s) 36, 347, 576
Bsp1286I GDGCHC 2 cut(s) 217, 263
Bsp143I GATC 6 cut(s) 165, 196, 474, 739, 838, 1039
Bsp19I CCATGG 2 cut(s) 208, 909
Bsp68I TCGCGA 1 cut(s) 159
BspACI CCGC 4 cut(s) 44, 71, 285, 655
BspANI GGCC 3 cut(s) 36, 347, 576
BspCNI CTCAG 4 cut(s) 51, 229, 328, 943
BspFNI CGCG 2 cut(s) 159, 806
BspPI GGATC 3 cut(s) 469, 747, 846
BspTI CTTAAG 1 cut(s) 563
BsrI ACTGG 4 cut(s) 155, 286, 848, 1000
BssECI CCNNGG 4 cut(s) 31, 208, 909, 1113
BssMI GATC 6 cut(s) 165, 196, 474, 739, 838, 1039
BssNI GRCGYC 2 cut(s) 59, 65
BssT1I CCWWGG 3 cut(s) 31, 208, 909
Bst2UI CCWGG 1 cut(s) 436
Bst4CI ACNGT 5 cut(s) 127, 258, 448, 921, 963
Bst6I CTCTTC 2 cut(s) 209, 837
BstACI GRCGYC 2 cut(s) 59, 65
BstAFI CTTAAG 1 cut(s) 563
BstC8I GCNNGC 4 cut(s) 552, 574, 928, 957
BstDEI CTNAG 5 cut(s) 38, 216, 315, 529, 951
BstDSI CCRYGG 2 cut(s) 208, 909
BstF5I GGATG 3 cut(s) 688, 1109, 1138
BstFNI CGCG 2 cut(s) 159, 806
BstHHI GCGC 2 cut(s) 30, 808
BstKTI GATC 6 cut(s) 168, 199, 477, 742, 841, 1042
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 6 cut(s) 165, 196, 474, 739, 838, 1039
BstMWI GCNNNNNNNGC 7 cut(s) 71, 113, 309, 392, 401, 531, 1115
BstNI CCWGG 1 cut(s) 436
BstNSI RCATGY 1 cut(s) 233
BstPAI GACNNNNGTC 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 434
BstSFI CTRYAG 1 cut(s) 289
BstSLI GKGCMC 1 cut(s) 263
BstUI CGCG 2 cut(s) 159, 806
BstV1I GCAGC 5 cut(s) 91, 395, 418, 509, 559
BstX2I RGATCY 1 cut(s) 474
BstYI RGATCY 1 cut(s) 474
Bsu36I CCTNAGG 1 cut(s) 529
BsuRI GGCC 3 cut(s) 36, 347, 576
BtgI CCRYGG 2 cut(s) 208, 909
BtsCI GGATG 3 cut(s) 688, 1109, 1138
BtsIMutI CAGTG 2 cut(s) 263, 959
BtuMI TCGCGA 1 cut(s) 159
Cac8I GCNNGC 4 cut(s) 552, 574, 928, 957
CaiI CAGNNNCTG 1 cut(s) 961
CfoI GCGC 2 cut(s) 30, 808
Cfr13I GGNCC 3 cut(s) 137, 346, 642
CseI GACGC 2 cut(s) 48, 54
Csp6I GTAC 1 cut(s) 19
CviAII CATG 7 cut(s) 209, 230, 424, 535, 874, 898, 910
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 5 cut(s) 38, 216, 315, 529, 951
DpnI GATC 6 cut(s) 167, 198, 476, 741, 840, 1041
DpnII GATC 6 cut(s) 165, 196, 474, 739, 838, 1039
Eam1104I CTCTTC 2 cut(s) 209, 837
EarI CTCTTC 2 cut(s) 209, 837
Ecl136II GAGCTC 1 cut(s) 215
Eco130I CCWWGG 3 cut(s) 31, 208, 909
Eco147I AGGCCT 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 217
Eco47I GGWCC 2 cut(s) 137, 642
Eco53kI GAGCTC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 818
Eco81I CCTNAGG 1 cut(s) 529
EcoICRI GAGCTC 1 cut(s) 215
EcoO109I RGGNCCY 2 cut(s) 137, 642
EcoRII CCWGG 1 cut(s) 434
EcoT14I CCWWGG 3 cut(s) 31, 208, 909
EcoT38I GRGCYC 1 cut(s) 217
ErhI CCWWGG 3 cut(s) 31, 208, 909
FaeI CATG 7 cut(s) 212, 233, 427, 538, 877, 901, 913
FalI AAGNNNNNCTT 2 cut(s) 449, 481
FaqI GGGAC 1 cut(s) 838
FatI CATG 7 cut(s) 208, 229, 423, 534, 873, 897, 909
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 7 cut(s) 72, 105, 286, 384, 432, 523, 548
FokI GGATG 3 cut(s) 695, 1096, 1145
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 7 cut(s) 72, 105, 286, 384, 432, 523, 548
FspBI CTAG 2 cut(s) 324, 599
GlaI GCGC 2 cut(s) 29, 807
GluI GCNGC 7 cut(s) 72, 105, 286, 384, 432, 523, 548
GsaI CCCAGC 1 cut(s) 978
GsuI CTGGAG 1 cut(s) 457
HaeIII GGCC 3 cut(s) 36, 347, 576
HapII CCGG 1 cut(s) 336
HgaI GACGC 2 cut(s) 48, 54
HhaI GCGC 2 cut(s) 30, 808
Hin1I GRCGYC 2 cut(s) 59, 65
Hin1II CATG 7 cut(s) 212, 233, 427, 538, 877, 901, 913
Hin6I GCGC 2 cut(s) 28, 806
HinP1I GCGC 2 cut(s) 28, 806
HindIII AAGCTT 2 cut(s) 353, 1122
HinfI GANTC 4 cut(s) 154, 182, 339, 1097
HpaII CCGG 1 cut(s) 336
HphI GGTGA 2 cut(s) 773, 1010
Hpy166II GTNNAC 3 cut(s) 178, 223, 261
Hpy188I TCNGA 5 cut(s) 41, 170, 917, 1093, 1114
Hpy188III TCNNGA 7 cut(s) 158, 194, 277, 324, 599, 705, 836
Hpy8I GTNNAC 3 cut(s) 178, 223, 261
Hpy99I CGWCG 6 cut(s) 52, 55, 58, 64, 70, 164
HpyCH4III ACNGT 5 cut(s) 127, 258, 448, 921, 963
HpyCH4V TGCA 8 cut(s) 107, 233, 253, 261, 423, 525, 686, 757
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 113, 309, 392, 401, 531, 1115
HpyF3I CTNAG 5 cut(s) 38, 216, 315, 529, 951
Hsp92I GRCGYC 2 cut(s) 59, 65
Hsp92II CATG 7 cut(s) 212, 233, 427, 538, 877, 901, 913
HspAI GCGC 2 cut(s) 28, 806
Kzo9I GATC 6 cut(s) 165, 196, 474, 739, 838, 1039
LmnI GCTCC 2 cut(s) 212, 308
Lsp1109I GCAGC 5 cut(s) 91, 395, 418, 509, 559
LweI GCATC 4 cut(s) 673, 1024, 1118, 1123
MaeI CTAG 2 cut(s) 324, 599
MalI GATC 6 cut(s) 167, 198, 476, 741, 840, 1041
MboI GATC 6 cut(s) 165, 196, 474, 739, 838, 1039
MboII GAAGA 4 cut(s) 84, 196, 465, 824
MfeI CAATTG 1 cut(s) 108
MflI RGATCY 1 cut(s) 474
MhlI GDGCHC 2 cut(s) 217, 263
MluCI AATT 7 cut(s) 108, 238, 502, 666, 690, 982, 1068
MlyI GAGTC 3 cut(s) 148, 176, 333
MroXI GAANNNNTTC 1 cut(s) 792
MseI TTAA 4 cut(s) 468, 557, 564, 669
MslI CAYNNNNRTG 1 cut(s) 228
MspA1I CMGCKG 2 cut(s) 104, 926
MspCI CTTAAG 1 cut(s) 563
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 1 cut(s) 436
MunI CAATTG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 436
MvnI CGCG 2 cut(s) 159, 806
MwoI GCNNNNNNNGC 7 cut(s) 71, 113, 309, 392, 401, 531, 1115
NcoI CCATGG 2 cut(s) 208, 909
NdeII GATC 6 cut(s) 165, 196, 474, 739, 838, 1039
NlaIII CATG 7 cut(s) 212, 233, 427, 538, 877, 901, 913
NruI TCGCGA 1 cut(s) 159
NspI RCATGY 1 cut(s) 233
PceI AGGCCT 1 cut(s) 36
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 62
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 1 cut(s) 1097
PkrI GCNGC 7 cut(s) 73, 106, 287, 385, 433, 524, 549
PleI GAGTC 3 cut(s) 148, 176, 333
PpsI GAGTC 3 cut(s) 148, 176, 333
PpuMI RGGWCCY 2 cut(s) 137, 642
PshAI GACNNNNGTC 1 cut(s) 46
PsiI TTATAA 1 cut(s) 588
Psp124BI GAGCTC 1 cut(s) 217
Psp5II RGGWCCY 2 cut(s) 137, 642
Psp6I CCWGG 1 cut(s) 434
PspFI CCCAGC 1 cut(s) 974
PspGI CCWGG 1 cut(s) 434
PspPI GGNCC 3 cut(s) 137, 346, 642
PspPPI RGGWCCY 2 cut(s) 137, 642
PstNI CAGNNNCTG 1 cut(s) 961
PsuI RGATCY 1 cut(s) 474
PvuII CAGCTG 2 cut(s) 104, 926
RruI TCGCGA 1 cut(s) 159
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
RseI CAYNNNNRTG 1 cut(s) 228
SacI GAGCTC 1 cut(s) 217
SaqAI TTAA 4 cut(s) 468, 557, 564, 669
SatI GCNGC 7 cut(s) 72, 105, 286, 384, 432, 523, 548
Sau3AI GATC 6 cut(s) 165, 196, 474, 739, 838, 1039
Sau96I GGNCC 3 cut(s) 137, 346, 642
SchI GAGTC 3 cut(s) 148, 176, 333
ScrFI CCNGG 1 cut(s) 436
SduI GDGCHC 2 cut(s) 217, 263
SfaNI GCATC 4 cut(s) 673, 1024, 1118, 1123
SfcI CTRYAG 1 cut(s) 289
SinI GGWCC 2 cut(s) 137, 642
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 3 cut(s) 341, 563, 607
SmoI CTYRAG 3 cut(s) 341, 563, 607
Sse9I AATT 7 cut(s) 108, 238, 502, 666, 690, 982, 1068
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 4 cut(s) 44, 71, 285, 655
SspI AATATT 2 cut(s) 617, 990
SspMI CTAG 2 cut(s) 324, 599
SstI GAGCTC 1 cut(s) 217
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 434
StyI CCWWGG 3 cut(s) 31, 208, 909
TaaI ACNGT 5 cut(s) 127, 258, 448, 921, 963
TaqI TCGA 3 cut(s) 142, 195, 742
TasI AATT 7 cut(s) 108, 238, 502, 666, 690, 982, 1068
TauI GCSGC 2 cut(s) 74, 288
TfiI GAWTC 1 cut(s) 1097
Tru1I TTAA 4 cut(s) 468, 557, 564, 669
Tru9I TTAA 4 cut(s) 468, 557, 564, 669
TscAI CASTG 2 cut(s) 263, 966
TseI GCWGC 5 cut(s) 104, 383, 431, 522, 547
TspDTI ATGAA 4 cut(s) 84, 374, 392, 1110
TspGWI ACGGA 2 cut(s) 904, 1132
TspRI CASTG 2 cut(s) 263, 966
Vha464I CTTAAG 1 cut(s) 563
VneI GTGCAC 1 cut(s) 259
VpaK11BI GGWCC 2 cut(s) 137, 642
XapI RAATTY 2 cut(s) 502, 982
XbaI TCTAGA 2 cut(s) 323, 598
XceI RCATGY 1 cut(s) 233
XcmI CCANNNNNNNNNTGG 1 cut(s) 855
XmiI GTMKAC 1 cut(s) 177
XmnI GAANNNNTTC 1 cut(s) 792
XspI CTAG 2 cut(s) 324, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.