RLG00000008670

Biotin synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015317
Physical Location & Seq
Reverse (-)
34684676 .. 34695380
10705 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008670

Sequence Viewer

Length: 1092 bp
ATGTTCTCCATAAGGTCCATTCTGCGCCAAGGCCTCAAACTGTCGGCGGCGGTGGCGGCATTTTACTCCTCTCTATCTTCATCAGTAGCCGCGATTGAAGCGGAGAGAACAGTTCTACAAGGTCCTCGAAATGACTGGACTCGCGACGAGATCAGAGCCGTCTACGACTCTCCTGTTCTCGATCTCCTCTTCCATGGAGCTCAGGTTCACAGACATACACATAATTTTACAGAAGTGCAACAGTGCACTCTCCTCTCTATCAAGACTGGTGGCTGTAGTGAGGATTGCTCCTATTGCCCTCAGTCCTCTAGATACAATACCGGACTCAAGGCACAAAAGCTTATGAATGAGGATGATTTAATGAAAGCAGCGAAAAAGGCAAAAGAGGCTAGTAGCACACGCTTTTGCATGGGTGCTGCCTGGAGGGAGACACTAGGAAGAAAGATAAACTTTAACCAGATCCTTGAATATGTGAAAGAAATAAGGAATTTGGGAATGGAAGTGTGCTGCACCTTAGAACAGCAGCAAGCGTTAAAACTTAAGGAAGCTGGCCTGATAGCTTATAATCACAATCTAGACACCTCAAGGGAATATTATCCCAATGTCATTACCACAAGGACCTATGATGACTGCTTGGAAACAATTAAGTATGTCAGGGATGCAGGAATTAGTGTCTGTTCAATTGGTTTGTTTCATACATTAGCAACACTTCCATCTCACCCAGAAAGTGTTCCCATAAATGCGCTGATTAGTGTGAAAGGGACACCTCTTCAGGATCAAAAGCCAGTTGAAATATGGGAGATGATACGCATGATTGCCACAGCACGCATAGTCATGCCAAAAGCCATGGTCAGACTGTCAGCTGGCAGAGTTCATTTTTCAATGCCTGAGCAGGCACTGTGCTTTCTTGCTGGGGCAAATTCAATATTTACTGGTGAAAAACTATTGACCACACCTAACAATGATTTTCATGTCGACCAAGTTATGTTCAAGGTGCTTGGATTAATTCCAAAACCTCCCAGTTTCTCCGATGAACCAGCAAAGGCATCCGAGGCAGAAGCTTGTGAGGATGCTGTTTCCGTTTCAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
BATS Radical_SAM

Protein Analysis

364

Amino Acids

40.28

Weight (kDa)

6.76

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 87 - 229 2.1e-17 Radical SAM superfamily
BATS PF06968 245 - 335 6e-26 Biotin and Thiamin Synthesis associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43360
fragaria_vesca FvH4_4g11460 FvH4_4g11460
malus_domestica MD04G1037300.v1.1
prunus_persica Prupe.1G186700_v2.0.a1
pyrus_communis pycom04g03040
rosa_chinensis RchiOBHm_Chr4g0408131
rosa_laevigata RLG00000008670 RLG00000008673
rosa_multiflora Rmu_sc0002073.1_g000002
rosa_roxburghii Rroxscaffold_5G00352620
rosa_rugosa Rorug04G0083700
rosa_samantha Rh4AG148100 Rh4BG145300 Rh4DG141800
rosa_wichuraiana Rw4G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 564
AccI GTMKAC 2 cut(s) 162, 975
AccII CGCG 2 cut(s) 92, 144
AciI CCGC 5 cut(s) 47, 50, 56, 90, 101
AclWI GGATC 2 cut(s) 454, 783
AcsI RAATTY 2 cut(s) 487, 919
AcuI CTGAAG 1 cut(s) 755
AflII CTTAAG 1 cut(s) 539
AgsI TTSAA 7 cut(s) 98, 467, 681, 791, 882, 924, 991
AjnI CCWGG 1 cut(s) 419
AloI GAACNNNNNNTCC 2 cut(s) 189, 221
AluBI AGCT 6 cut(s) 200, 340, 548, 560, 863, 1061
AluI AGCT 6 cut(s) 200, 340, 548, 560, 863, 1061
Alw21I GWGCWC 2 cut(s) 202, 248
Alw26I GTCTC 1 cut(s) 422
Alw44I GTGCAC 1 cut(s) 244
AlwI GGATC 2 cut(s) 454, 783
AlwNI CAGNNNCTG 1 cut(s) 898
AoxI GGCC 2 cut(s) 31, 550
ApaLI GTGCAC 1 cut(s) 244
ApeKI GCWGC 4 cut(s) 368, 416, 507, 523
ApoI RAATTY 2 cut(s) 487, 919
AseI ATTAAT 1 cut(s) 1004
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 2 cut(s) 27, 745
AspS9I GGNCC 3 cut(s) 15, 122, 618
AsuHPI GGTGA 2 cut(s) 710, 947
AvaII GGWCC 3 cut(s) 15, 122, 618
BaeGI GKGCMC 1 cut(s) 248
BanII GRGCYC 1 cut(s) 202
Bbv12I GWGCWC 2 cut(s) 202, 248
BbvI GCAGC 4 cut(s) 380, 403, 494, 535
BccI CCATC 1 cut(s) 721
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 4 cut(s) 309, 390, 434, 575
BfmI CTRYAG 1 cut(s) 274
BfrI CTTAAG 1 cut(s) 539
BisI GCNGC 7 cut(s) 48, 57, 90, 369, 417, 508, 524
BlsI GCNGC 7 cut(s) 49, 58, 91, 370, 418, 509, 525
Bme1390I CCNGG 1 cut(s) 421
Bme18I GGWCC 3 cut(s) 15, 122, 618
BmgT120I GGNCC 3 cut(s) 15, 122, 618
BmrFI CCNGG 1 cut(s) 421
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 3 cut(s) 649, 1055, 1060
BmuI ACTGGG 1 cut(s) 1014
BplI GAGNNNNNCTC 2 cut(s) 272, 304
BpmI CTGGAG 1 cut(s) 442
Bpu10I CCTNAGC 2 cut(s) 201, 888
BpuEI CTTGAG 2 cut(s) 311, 568
BsaJI CCNNGG 4 cut(s) 28, 193, 846, 1050
BsaWI WCCGGW 1 cut(s) 320
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bse1I ACTGG 5 cut(s) 140, 271, 785, 937, 1020
BseBI CCWGG 1 cut(s) 421
BseDI CCNNGG 4 cut(s) 28, 193, 846, 1050
BseGI GGATG 4 cut(s) 358, 664, 1046, 1075
BseMII CTCAG 3 cut(s) 215, 314, 879
BseNI ACTGG 5 cut(s) 140, 271, 785, 937, 1020
BseRI GAGGAG 3 cut(s) 58, 176, 242
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 4 cut(s) 380, 403, 494, 535
BseYI CCCAGC 1 cut(s) 911
BsgI GTGCAG 1 cut(s) 493
Bsh1236I CGCG 2 cut(s) 92, 144
BshFI GGCC 2 cut(s) 33, 552
BsiHKAI GWGCWC 2 cut(s) 202, 248
BsiSI CCGG 1 cut(s) 321
BslFI GGGAC 1 cut(s) 775
BsmAI GTCTC 1 cut(s) 422
BsmFI GGGAC 1 cut(s) 775
BsnI GGCC 2 cut(s) 33, 552
Bsp1286I GDGCHC 2 cut(s) 202, 248
Bsp143I GATC 4 cut(s) 150, 181, 459, 775
Bsp19I CCATGG 2 cut(s) 193, 846
Bsp68I TCGCGA 1 cut(s) 144
BspACI CCGC 5 cut(s) 47, 50, 56, 90, 101
BspANI GGCC 2 cut(s) 33, 552
BspCNI CTCAG 3 cut(s) 214, 313, 880
BspFNI CGCG 2 cut(s) 92, 144
BspPI GGATC 2 cut(s) 454, 783
BspTI CTTAAG 1 cut(s) 539
BsrI ACTGG 5 cut(s) 140, 271, 785, 937, 1020
BssECI CCNNGG 4 cut(s) 28, 193, 846, 1050
BssMI GATC 4 cut(s) 150, 181, 459, 775
BssT1I CCWWGG 3 cut(s) 28, 193, 846
Bst2UI CCWGG 1 cut(s) 421
Bst4CI ACNGT 5 cut(s) 42, 112, 243, 858, 900
Bst6I CTCTTC 2 cut(s) 194, 774
BstAFI CTTAAG 1 cut(s) 539
BstC8I GCNNGC 5 cut(s) 528, 550, 826, 865, 894
BstDEI CTNAG 4 cut(s) 201, 300, 514, 888
BstDSI CCRYGG 2 cut(s) 193, 846
BstF5I GGATG 4 cut(s) 358, 664, 1046, 1075
BstFNI CGCG 2 cut(s) 92, 144
BstHHI GCGC 2 cut(s) 27, 745
BstKTI GATC 4 cut(s) 153, 184, 462, 778
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 4 cut(s) 150, 181, 459, 775
BstMWI GCNNNNNNNGC 7 cut(s) 53, 56, 98, 294, 377, 386, 1052
BstNI CCWGG 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 419
BstSFI CTRYAG 1 cut(s) 274
BstSLI GKGCMC 1 cut(s) 248
BstUI CGCG 2 cut(s) 92, 144
BstV1I GCAGC 4 cut(s) 380, 403, 494, 535
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BsuRI GGCC 2 cut(s) 33, 552
BtgI CCRYGG 2 cut(s) 193, 846
BtsCI GGATG 4 cut(s) 358, 664, 1046, 1075
BtsIMutI CAGTG 2 cut(s) 248, 896
BtuMI TCGCGA 1 cut(s) 144
Cac8I GCNNGC 5 cut(s) 528, 550, 826, 865, 894
CaiI CAGNNNCTG 1 cut(s) 898
CfoI GCGC 2 cut(s) 27, 745
Cfr13I GGNCC 3 cut(s) 15, 122, 618
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviAII CATG 6 cut(s) 194, 409, 811, 835, 847, 971
DdeI CTNAG 4 cut(s) 201, 300, 514, 888
DpnI GATC 4 cut(s) 152, 183, 461, 777
DpnII GATC 4 cut(s) 150, 181, 459, 775
Eam1104I CTCTTC 2 cut(s) 194, 774
EarI CTCTTC 2 cut(s) 194, 774
Ecl136II GAGCTC 1 cut(s) 200
Eco130I CCWWGG 3 cut(s) 28, 193, 846
Eco147I AGGCCT 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 202
Eco47I GGWCC 3 cut(s) 15, 122, 618
Eco53kI GAGCTC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 755
EcoICRI GAGCTC 1 cut(s) 200
EcoO109I RGGNCCY 2 cut(s) 122, 618
EcoRII CCWGG 1 cut(s) 419
EcoT14I CCWWGG 3 cut(s) 28, 193, 846
EcoT38I GRGCYC 1 cut(s) 202
ErhI CCWWGG 3 cut(s) 28, 193, 846
FaeI CATG 6 cut(s) 197, 412, 814, 838, 850, 974
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FaqI GGGAC 1 cut(s) 775
FatI CATG 6 cut(s) 193, 408, 810, 834, 846, 970
FblI GTMKAC 2 cut(s) 162, 975
Fnu4HI GCNGC 7 cut(s) 48, 57, 90, 369, 417, 508, 524
FokI GGATG 4 cut(s) 365, 671, 1033, 1082
FriOI GRGCYC 1 cut(s) 202
Fsp4HI GCNGC 7 cut(s) 48, 57, 90, 369, 417, 508, 524
FspBI CTAG 4 cut(s) 309, 390, 434, 575
GlaI GCGC 2 cut(s) 26, 744
GluI GCNGC 7 cut(s) 48, 57, 90, 369, 417, 508, 524
GsaI CCCAGC 1 cut(s) 915
GsuI CTGGAG 1 cut(s) 442
HaeIII GGCC 2 cut(s) 33, 552
HapII CCGG 1 cut(s) 321
HhaI GCGC 2 cut(s) 27, 745
Hin1II CATG 6 cut(s) 197, 412, 814, 838, 850, 974
Hin6I GCGC 2 cut(s) 25, 743
HinP1I GCGC 2 cut(s) 25, 743
HincII GTYRAC 1 cut(s) 976
HindII GTYRAC 1 cut(s) 976
HindIII AAGCTT 2 cut(s) 338, 1059
HinfI GANTC 3 cut(s) 139, 167, 324
HpaII CCGG 1 cut(s) 321
HphI GGTGA 2 cut(s) 710, 947
Hpy166II GTNNAC 4 cut(s) 163, 208, 246, 976
Hpy188I TCNGA 4 cut(s) 155, 854, 1030, 1051
Hpy188III TCNNGA 6 cut(s) 143, 179, 262, 309, 575, 773
Hpy8I GTNNAC 4 cut(s) 163, 208, 246, 976
Hpy99I CGWCG 1 cut(s) 149
HpyCH4III ACNGT 5 cut(s) 42, 112, 243, 858, 900
HpyCH4V TGCA 5 cut(s) 238, 246, 408, 510, 662
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 56, 98, 294, 377, 386, 1052
HpyF3I CTNAG 4 cut(s) 201, 300, 514, 888
Hsp92II CATG 6 cut(s) 197, 412, 814, 838, 850, 974
HspAI GCGC 2 cut(s) 25, 743
Kzo9I GATC 4 cut(s) 150, 181, 459, 775
LmnI GCTCC 2 cut(s) 197, 293
Lsp1109I GCAGC 4 cut(s) 380, 403, 494, 535
LweI GCATC 3 cut(s) 649, 1055, 1060
MaeI CTAG 4 cut(s) 309, 390, 434, 575
MalI GATC 4 cut(s) 152, 183, 461, 777
MboI GATC 4 cut(s) 150, 181, 459, 775
MboII GAAGA 4 cut(s) 69, 181, 450, 761
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 2 cut(s) 202, 248
MluCI AATT 7 cut(s) 223, 487, 642, 666, 681, 919, 1005
MlyI GAGTC 3 cut(s) 133, 161, 318
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 6 cut(s) 359, 453, 533, 540, 645, 1004
MslI CAYNNNNRTG 1 cut(s) 833
MspA1I CMGCKG 1 cut(s) 863
MspCI CTTAAG 1 cut(s) 539
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 421
MunI CAATTG 1 cut(s) 681
MvaI CCWGG 1 cut(s) 421
MvnI CGCG 2 cut(s) 92, 144
MwoI GCNNNNNNNGC 7 cut(s) 53, 56, 98, 294, 377, 386, 1052
NcoI CCATGG 2 cut(s) 193, 846
NdeII GATC 4 cut(s) 150, 181, 459, 775
NlaIII CATG 6 cut(s) 197, 412, 814, 838, 850, 974
NruI TCGCGA 1 cut(s) 144
PceI AGGCCT 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 729
PkrI GCNGC 7 cut(s) 49, 58, 91, 370, 418, 509, 525
PleI GAGTC 3 cut(s) 133, 161, 318
PpsI GAGTC 3 cut(s) 133, 161, 318
PpuMI RGGWCCY 2 cut(s) 122, 618
PshBI ATTAAT 1 cut(s) 1004
PsiI TTATAA 1 cut(s) 564
Psp124BI GAGCTC 1 cut(s) 202
Psp5II RGGWCCY 2 cut(s) 122, 618
Psp6I CCWGG 1 cut(s) 419
PspFI CCCAGC 1 cut(s) 911
PspGI CCWGG 1 cut(s) 419
PspPI GGNCC 3 cut(s) 15, 122, 618
PspPPI RGGWCCY 2 cut(s) 122, 618
PstNI CAGNNNCTG 1 cut(s) 898
PsuI RGATCY 1 cut(s) 459
PvuII CAGCTG 1 cut(s) 863
RruI TCGCGA 1 cut(s) 144
RseI CAYNNNNRTG 1 cut(s) 833
SacI GAGCTC 1 cut(s) 202
SalI GTCGAC 1 cut(s) 974
SaqAI TTAA 6 cut(s) 359, 453, 533, 540, 645, 1004
SatI GCNGC 7 cut(s) 48, 57, 90, 369, 417, 508, 524
Sau3AI GATC 4 cut(s) 150, 181, 459, 775
Sau96I GGNCC 3 cut(s) 15, 122, 618
SchI GAGTC 3 cut(s) 133, 161, 318
ScrFI CCNGG 1 cut(s) 421
SduI GDGCHC 2 cut(s) 202, 248
SfaNI GCATC 3 cut(s) 649, 1055, 1060
SfcI CTRYAG 1 cut(s) 274
SinI GGWCC 3 cut(s) 15, 122, 618
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 3 cut(s) 326, 539, 583
SmoI CTYRAG 3 cut(s) 326, 539, 583
Sse9I AATT 7 cut(s) 223, 487, 642, 666, 681, 919, 1005
SseBI AGGCCT 1 cut(s) 33
SsiI CCGC 5 cut(s) 47, 50, 56, 90, 101
SspI AATATT 2 cut(s) 593, 927
SspMI CTAG 4 cut(s) 309, 390, 434, 575
SstI GAGCTC 1 cut(s) 202
StuI AGGCCT 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 419
StyI CCWWGG 3 cut(s) 28, 193, 846
TaaI ACNGT 5 cut(s) 42, 112, 243, 858, 900
TaqI TCGA 3 cut(s) 127, 180, 975
TasI AATT 7 cut(s) 223, 487, 642, 666, 681, 919, 1005
TauI GCSGC 3 cut(s) 50, 59, 92
Tru1I TTAA 6 cut(s) 359, 453, 533, 540, 645, 1004
Tru9I TTAA 6 cut(s) 359, 453, 533, 540, 645, 1004
TscAI CASTG 2 cut(s) 248, 903
TseI GCWGC 4 cut(s) 368, 416, 507, 523
TspDTI ATGAA 7 cut(s) 69, 359, 377, 683, 863, 959, 1047
TspGWI ACGGA 1 cut(s) 1069
TspRI CASTG 2 cut(s) 248, 903
Vha464I CTTAAG 1 cut(s) 539
VneI GTGCAC 1 cut(s) 244
VpaK11BI GGWCC 3 cut(s) 15, 122, 618
VspI ATTAAT 1 cut(s) 1004
XapI RAATTY 2 cut(s) 487, 919
XbaI TCTAGA 2 cut(s) 308, 574
XcmI CCANNNNNNNNNTGG 1 cut(s) 792
XmiI GTMKAC 2 cut(s) 162, 975
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 4 cut(s) 309, 390, 434, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.