Rroxscaffold_5G00352620

Biotin synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015317
Physical Location & Seq
Forward (+)
29694429 .. 29700027
5599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352620.1

Sequence Viewer

Length: 1152 bp
ATGATGTTTTCCATAAGGTCCATTCTGCGCCAAGGCCTCAGACCGCCGCCGTCGTCGTTGGCGTCGGCGGCATTTTACTCCTCTCTATCTTCATCAGCAGCTGCAATTGAAGCAGAGAGAGCAGTCCTACAAGGTCCTCGAAATGACTGGACTCGCGACGAGATCAGAGCCGTCTACGACTCTCCTGTTCTCGATCTCCTCTTCCATGGAGCTCAGGTTCACAGACATACACATAATTTTAGAGAAGTGCAACAGTGCACTCTCCTCTCTATCAAGACTGGTGGCTGTAGTGAGGATTGCTCCTATTGCCCTCAGTCCTCTAGATACGATACCGGACTCAAGGCACAAAAGCTTATGAATAAGGACGATGTAATGAAAGCAGCGAAAAAGGCAAAAGAGGCTGGTAGCACACGCTTTTGCATGGGTGCTGCCTGGAGGGAGACAGTAGGAAGAAAGACAAACTTTAACCAGATCCTTGAATATGTGAAAGAAATAAGGAATTTGGGAATGGAAGTGTGCTGCACCTTAGGCATGCTAGAGCAGCAGCAAGCGTTAAAACTTAAGGAAGCTGGCCTGACAGCTTATAATCACAATCTAGACACCTCAAGGGAATATTATCCCAATATCATTACCACAAGGACCTATGATGACCGCTTGGAAACAATTAAGTATGTCAGGGATGCAGGAATTAGTGTGTGTTCAGGAGGAATAATAGGGCTCGGAGAAGCAGCGGAGGATCGAGTTGGTTTGTTGCATACATTAGCAACACTTCCATCTCACCCAGAAAGTGTTCCCATAAATGCGCTGATTAGTGTGAAGGGGACACCTCTTCAAGATCAAAAGCCAGTTGAAATATGGGAAATGATACGCATGATTGCCACAGCACGTATAGTCATGCCAAAAGCCATGGTCAGACTGTCAGCTGGCAGAGTTCGTTTCTCAATGCCTGAGCAGGCACTATGCTTTCTTGCTGGGGCAAATTCAATATTTACTGGTGAAAAACTATTGACCACACCTAACAATGATTTTGATGCCGATCAAGTTATGTTCAAGGTGCTTGGACTAATTCCAAAACCTCCCAGTTTCTCCGATGAACCAGCAAAGGCATCCGACGCAGAAGCTTGTGAGGATGCTGTTTCCGTTTCAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
BATS Radical_SAM

Protein Analysis

383

Amino Acids

42.16

Weight (kDa)

7.03

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 91 - 252 3e-22 Radical SAM superfamily
BATS PF06968 265 - 355 6.6e-27 Biotin and Thiamin Synthesis associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43360
fragaria_vesca FvH4_4g11460 FvH4_4g11460
malus_domestica MD04G1037300.v1.1
prunus_persica Prupe.1G186700_v2.0.a1
pyrus_communis pycom04g03040
rosa_chinensis RchiOBHm_Chr4g0408131
rosa_laevigata RLG00000008670 RLG00000008673
rosa_multiflora Rmu_sc0002073.1_g000002
rosa_roxburghii Rroxscaffold_5G00352620
rosa_rugosa Rorug04G0083700
rosa_samantha Rh4AG148100 Rh4BG145300 Rh4DG141800
rosa_wichuraiana Rw4G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AccI GTMKAC 1 cut(s) 174
AccII CGCG 1 cut(s) 156
AciI CCGC 5 cut(s) 44, 47, 68, 652, 731
AclWI GGATC 2 cut(s) 466, 744
AcsI RAATTY 2 cut(s) 499, 979
AcyI GRCGYC 1 cut(s) 62
AflII CTTAAG 1 cut(s) 560
AgsI TTSAA 6 cut(s) 110, 479, 833, 851, 984, 1051
AjnI CCWGG 1 cut(s) 431
AloI GAACNNNNNNTCC 2 cut(s) 201, 233
AluBI AGCT 7 cut(s) 101, 212, 352, 569, 581, 923, 1121
AluI AGCT 7 cut(s) 101, 212, 352, 569, 581, 923, 1121
Alw21I GWGCWC 2 cut(s) 214, 260
Alw26I GTCTC 1 cut(s) 434
Alw44I GTGCAC 1 cut(s) 256
AlwI GGATC 2 cut(s) 466, 744
AlwNI CAGNNNCTG 1 cut(s) 101
AoxI GGCC 2 cut(s) 34, 571
ApaLI GTGCAC 1 cut(s) 256
ApeKI GCWGC 8 cut(s) 98, 101, 380, 428, 519, 541, 544, 728
ApoI RAATTY 2 cut(s) 499, 979
Asp700I GAANNNNTTC 1 cut(s) 789
AspLEI GCGC 2 cut(s) 30, 805
AspS9I GGNCC 3 cut(s) 18, 134, 639
AsuHPI GGTGA 2 cut(s) 770, 1007
AvaII GGWCC 3 cut(s) 18, 134, 639
AxyI CCTNAGG 1 cut(s) 526
BaeGI GKGCMC 1 cut(s) 260
BanII GRGCYC 2 cut(s) 214, 720
Bbv12I GWGCWC 2 cut(s) 214, 260
BbvI GCAGC 8 cut(s) 88, 110, 392, 415, 506, 553, 556, 740
BccI CCATC 1 cut(s) 781
BceAI ACGGC 2 cut(s) 34, 155
BciT130I CCWGG 1 cut(s) 433
BcoDI GTCTC 1 cut(s) 434
BfaI CTAG 3 cut(s) 321, 536, 596
BfmI CTRYAG 1 cut(s) 286
BfrI CTTAAG 1 cut(s) 560
Bme1390I CCNGG 1 cut(s) 433
Bme18I GGWCC 3 cut(s) 18, 134, 639
BmgT120I GGNCC 3 cut(s) 18, 134, 639
BmrFI CCNGG 1 cut(s) 433
BmrI ACTGGG 1 cut(s) 1074
BmsI GCATC 4 cut(s) 670, 1021, 1115, 1120
BmuI ACTGGG 1 cut(s) 1074
BplI GAGNNNNNCTC 2 cut(s) 284, 316
BpmI CTGGAG 1 cut(s) 454
Bpu10I CCTNAGC 2 cut(s) 213, 948
BpuEI CTTGAG 2 cut(s) 323, 589
BsaAI YACGTR 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 1035
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 31, 205, 906
BsaWI WCCGGW 1 cut(s) 332
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bse1I ACTGG 5 cut(s) 152, 283, 845, 997, 1080
Bse21I CCTNAGG 1 cut(s) 526
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 1 cut(s) 433
BseDI CCNNGG 3 cut(s) 31, 205, 906
BseGI GGATG 3 cut(s) 685, 1106, 1135
BseJI GATNNNNATC 1 cut(s) 1035
BseMII CTCAG 4 cut(s) 52, 227, 326, 939
BseNI ACTGG 5 cut(s) 152, 283, 845, 997, 1080
BseRI GAGGAG 3 cut(s) 70, 188, 254
BseSI GKGCMC 1 cut(s) 260
BseXI GCAGC 8 cut(s) 88, 110, 392, 415, 506, 553, 556, 740
BseYI CCCAGC 1 cut(s) 971
BsgI GTGCAG 1 cut(s) 505
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 2 cut(s) 36, 573
BsiHKAI GWGCWC 2 cut(s) 214, 260
BsiSI CCGG 1 cut(s) 333
BslFI GGGAC 1 cut(s) 835
BsmAI GTCTC 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 835
BsnI GGCC 2 cut(s) 36, 573
Bsp1286I GDGCHC 3 cut(s) 214, 260, 720
Bsp143I GATC 6 cut(s) 162, 193, 471, 736, 835, 1036
Bsp19I CCATGG 2 cut(s) 205, 906
Bsp68I TCGCGA 1 cut(s) 156
BspACI CCGC 5 cut(s) 44, 47, 68, 652, 731
BspANI GGCC 2 cut(s) 36, 573
BspCNI CTCAG 4 cut(s) 51, 226, 325, 940
BspFNI CGCG 1 cut(s) 156
BspPI GGATC 2 cut(s) 466, 744
BspTI CTTAAG 1 cut(s) 560
BsrI ACTGG 5 cut(s) 152, 283, 845, 997, 1080
BssECI CCNNGG 3 cut(s) 31, 205, 906
BssMI GATC 6 cut(s) 162, 193, 471, 736, 835, 1036
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 3 cut(s) 31, 205, 906
Bst2UI CCWGG 1 cut(s) 433
Bst4CI ACNGT 3 cut(s) 255, 445, 918
Bst6I CTCTTC 2 cut(s) 206, 834
BstACI GRCGYC 1 cut(s) 62
BstAFI CTTAAG 1 cut(s) 560
BstBAI YACGTR 1 cut(s) 887
BstC8I GCNNGC 5 cut(s) 533, 549, 571, 925, 954
BstDEI CTNAG 5 cut(s) 38, 213, 312, 526, 948
BstDSI CCRYGG 2 cut(s) 205, 906
BstF5I GGATG 3 cut(s) 685, 1106, 1135
BstFNI CGCG 1 cut(s) 156
BstHHI GCGC 2 cut(s) 30, 805
BstKTI GATC 6 cut(s) 165, 196, 474, 739, 838, 1039
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 6 cut(s) 162, 193, 471, 736, 835, 1036
BstMWI GCNNNNNNNGC 9 cut(s) 68, 110, 119, 306, 389, 398, 528, 541, 1112
BstNI CCWGG 1 cut(s) 433
BstNSI RCATGY 1 cut(s) 535
BstSCI CCNGG 1 cut(s) 431
BstSFI CTRYAG 1 cut(s) 286
BstSLI GKGCMC 1 cut(s) 260
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 8 cut(s) 88, 110, 392, 415, 506, 553, 556, 740
BstX2I RGATCY 1 cut(s) 471
BstYI RGATCY 1 cut(s) 471
Bsu36I CCTNAGG 1 cut(s) 526
BsuRI GGCC 2 cut(s) 36, 573
BtgI CCRYGG 2 cut(s) 205, 906
BtsCI GGATG 3 cut(s) 685, 1106, 1135
BtsIMutI CAGTG 1 cut(s) 260
BtuMI TCGCGA 1 cut(s) 156
Cac8I GCNNGC 5 cut(s) 533, 549, 571, 925, 954
CaiI CAGNNNCTG 1 cut(s) 101
CfoI GCGC 2 cut(s) 30, 805
Cfr13I GGNCC 3 cut(s) 18, 134, 639
CseI GACGC 2 cut(s) 51, 1121
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
CviAII CATG 6 cut(s) 206, 421, 532, 871, 895, 907
DdeI CTNAG 5 cut(s) 38, 213, 312, 526, 948
DpnI GATC 6 cut(s) 164, 195, 473, 738, 837, 1038
DpnII GATC 6 cut(s) 162, 193, 471, 736, 835, 1036
Eam1104I CTCTTC 2 cut(s) 206, 834
EarI CTCTTC 2 cut(s) 206, 834
Ecl136II GAGCTC 1 cut(s) 212
Eco130I CCWWGG 3 cut(s) 31, 205, 906
Eco147I AGGCCT 1 cut(s) 36
Eco24I GRGCYC 2 cut(s) 214, 720
Eco47I GGWCC 3 cut(s) 18, 134, 639
Eco53kI GAGCTC 1 cut(s) 212
Eco81I CCTNAGG 1 cut(s) 526
EcoICRI GAGCTC 1 cut(s) 212
EcoO109I RGGNCCY 2 cut(s) 134, 639
EcoRII CCWGG 1 cut(s) 431
EcoT14I CCWWGG 3 cut(s) 31, 205, 906
EcoT38I GRGCYC 2 cut(s) 214, 720
ErhI CCWWGG 3 cut(s) 31, 205, 906
FaeI CATG 6 cut(s) 209, 424, 535, 874, 898, 910
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 1 cut(s) 835
FatI CATG 6 cut(s) 205, 420, 531, 870, 894, 906
FblI GTMKAC 1 cut(s) 174
FokI GGATG 3 cut(s) 692, 1093, 1142
FriOI GRGCYC 2 cut(s) 214, 720
FspBI CTAG 3 cut(s) 321, 536, 596
GlaI GCGC 2 cut(s) 29, 804
GsaI CCCAGC 1 cut(s) 975
GsuI CTGGAG 1 cut(s) 454
HaeIII GGCC 2 cut(s) 36, 573
HapII CCGG 1 cut(s) 333
HgaI GACGC 2 cut(s) 51, 1121
HhaI GCGC 2 cut(s) 30, 805
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 6 cut(s) 209, 424, 535, 874, 898, 910
Hin6I GCGC 2 cut(s) 28, 803
HinP1I GCGC 2 cut(s) 28, 803
HindIII AAGCTT 2 cut(s) 350, 1119
HinfI GANTC 3 cut(s) 151, 179, 336
HpaII CCGG 1 cut(s) 333
HphI GGTGA 2 cut(s) 770, 1007
Hpy166II GTNNAC 3 cut(s) 175, 220, 258
Hpy188I TCNGA 6 cut(s) 41, 167, 722, 914, 1090, 1111
Hpy188III TCNNGA 7 cut(s) 155, 191, 274, 321, 596, 702, 833
Hpy8I GTNNAC 3 cut(s) 175, 220, 258
Hpy99I CGWCG 5 cut(s) 55, 58, 67, 161, 1115
HpyAV CCTTC 1 cut(s) 811
HpyCH4III ACNGT 3 cut(s) 255, 445, 918
HpyCH4IV ACGT 1 cut(s) 886
HpyCH4V TGCA 7 cut(s) 104, 250, 258, 420, 522, 683, 754
HpyF10VI GCNNNNNNNGC 9 cut(s) 68, 110, 119, 306, 389, 398, 528, 541, 1112
HpyF3I CTNAG 5 cut(s) 38, 213, 312, 526, 948
HpySE526I ACGT 1 cut(s) 886
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 6 cut(s) 209, 424, 535, 874, 898, 910
HspAI GCGC 2 cut(s) 28, 803
Kzo9I GATC 6 cut(s) 162, 193, 471, 736, 835, 1036
LmnI GCTCC 2 cut(s) 209, 305
Lsp1109I GCAGC 8 cut(s) 88, 110, 392, 415, 506, 553, 556, 740
LweI GCATC 4 cut(s) 670, 1021, 1115, 1120
MaeI CTAG 3 cut(s) 321, 536, 596
MaeII ACGT 1 cut(s) 886
MalI GATC 6 cut(s) 164, 195, 473, 738, 837, 1038
MboI GATC 6 cut(s) 162, 193, 471, 736, 835, 1036
MboII GAAGA 4 cut(s) 81, 193, 462, 821
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 3 cut(s) 214, 260, 720
MluCI AATT 7 cut(s) 105, 235, 499, 663, 687, 979, 1065
MlyI GAGTC 3 cut(s) 145, 173, 330
MmeI TCCRAC 1 cut(s) 1134
MroXI GAANNNNTTC 1 cut(s) 789
MseI TTAA 4 cut(s) 465, 554, 561, 666
MspA1I CMGCKG 3 cut(s) 101, 731, 923
MspCI CTTAAG 1 cut(s) 560
MspI CCGG 1 cut(s) 333
MspR9I CCNGG 1 cut(s) 433
MunI CAATTG 1 cut(s) 105
MvaI CCWGG 1 cut(s) 433
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 9 cut(s) 68, 110, 119, 306, 389, 398, 528, 541, 1112
NcoI CCATGG 2 cut(s) 205, 906
NdeII GATC 6 cut(s) 162, 193, 471, 736, 835, 1036
NlaIII CATG 6 cut(s) 209, 424, 535, 874, 898, 910
NruI TCGCGA 1 cut(s) 156
NspI RCATGY 1 cut(s) 535
PaeI GCATGC 1 cut(s) 535
PceI AGGCCT 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PdmI GAANNNNTTC 1 cut(s) 789
PleI GAGTC 3 cut(s) 145, 173, 330
PpsI GAGTC 3 cut(s) 145, 173, 330
Ppu21I YACGTR 1 cut(s) 887
PpuMI RGGWCCY 2 cut(s) 134, 639
PsiI TTATAA 1 cut(s) 585
Psp124BI GAGCTC 1 cut(s) 214
Psp5II RGGWCCY 2 cut(s) 134, 639
Psp6I CCWGG 1 cut(s) 431
PspFI CCCAGC 1 cut(s) 971
PspGI CCWGG 1 cut(s) 431
PspPI GGNCC 3 cut(s) 18, 134, 639
PspPPI RGGWCCY 2 cut(s) 134, 639
PstNI CAGNNNCTG 1 cut(s) 101
PsuI RGATCY 1 cut(s) 471
PvuII CAGCTG 2 cut(s) 101, 923
RruI TCGCGA 1 cut(s) 156
SacI GAGCTC 1 cut(s) 214
SaqAI TTAA 4 cut(s) 465, 554, 561, 666
Sau3AI GATC 6 cut(s) 162, 193, 471, 736, 835, 1036
Sau96I GGNCC 3 cut(s) 18, 134, 639
SchI GAGTC 3 cut(s) 145, 173, 330
ScrFI CCNGG 1 cut(s) 433
SduI GDGCHC 3 cut(s) 214, 260, 720
SfaNI GCATC 4 cut(s) 670, 1021, 1115, 1120
SfcI CTRYAG 1 cut(s) 286
SinI GGWCC 3 cut(s) 18, 134, 639
SmlI CTYRAG 3 cut(s) 338, 560, 604
SmoI CTYRAG 3 cut(s) 338, 560, 604
SphI GCATGC 1 cut(s) 535
Sse9I AATT 7 cut(s) 105, 235, 499, 663, 687, 979, 1065
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 5 cut(s) 44, 47, 68, 652, 731
SspI AATATT 2 cut(s) 614, 987
SspMI CTAG 3 cut(s) 321, 536, 596
SstI GAGCTC 1 cut(s) 214
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 431
StyI CCWWGG 3 cut(s) 31, 205, 906
TaaI ACNGT 3 cut(s) 255, 445, 918
TaiI ACGT 1 cut(s) 889
TaqI TCGA 3 cut(s) 139, 192, 739
TasI AATT 7 cut(s) 105, 235, 499, 663, 687, 979, 1065
TauI GCSGC 2 cut(s) 49, 71
Tru1I TTAA 4 cut(s) 465, 554, 561, 666
Tru9I TTAA 4 cut(s) 465, 554, 561, 666
TscAI CASTG 1 cut(s) 260
TseI GCWGC 8 cut(s) 98, 101, 380, 428, 519, 541, 544, 728
TspDTI ATGAA 4 cut(s) 81, 371, 389, 1107
TspGWI ACGGA 1 cut(s) 1129
TspRI CASTG 1 cut(s) 260
Vha464I CTTAAG 1 cut(s) 560
VneI GTGCAC 1 cut(s) 256
VpaK11BI GGWCC 3 cut(s) 18, 134, 639
XapI RAATTY 2 cut(s) 499, 979
XbaI TCTAGA 2 cut(s) 320, 595
XceI RCATGY 1 cut(s) 535
XcmI CCANNNNNNNNNTGG 1 cut(s) 852
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 789
XspI CTAG 3 cut(s) 321, 536, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.