MD04G1037300.v1.1

Biotin synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015317
Physical Location & Seq
Reverse (-)
4158558 .. 4176911
18354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1037300.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGTTTTCCGTTAGGTCCGTAGTGCGTGGGCAGCTGAGACCGTCGGCGGTTGTGTTGTTGCATTCTTCATTTTACTCTTCCTTATCGTCGGCAGCTGCAGTTCAAGCTGAGAGAACAATCGCACAAGGCCCTAGAAATGACTGGACCCGCGACGAGATCAAGGACGTCTACGGCTCCCCTGTTCTCGATCTCCTTTTTCACGGAGCTCAAGTTCATAGACACGCTCATAATTTTAGGGAAGTGCAGCAGTGTACTCTCCTCTCTATAAAGACGGGTGGATGTAGTGAGGATTGCTCATATTGTCCTCAATCCTCCAGGTATGACACAGGACTCAAGGCCCAAAAGCTTATGACAAAGGATGCTGTCATGGAAGCAGCACAAAGGGCAAAAGAGGCTGGAAGCACACGGTTTTGCATGGGTGCTGCCTGGAGGGATACAATAGGAAGAAAGACAAACTTTAACCAGATCCTTGAATATGTAAAAGACATAAGGGGTATGGGAATGGAGGTCTGCTGCACCTTAGGCATGATAGAAAAGCAACAAGCTTTGAAACTCAAAGAAGCAGGCCTGACAGCTTATAATCACAATCTTGACACCTCTAGGGAATATTACCCGAATATCATTACCACAAGGACCTATGATGAGCGCTTGGAAACCATTAAGCATGTCCGGGATGCAGGAATTAATGTGTGTTCAGGAGGAATAATAGGACTTGGAGAAGCAGAGGAGGATCGAGTTGGTTTGCTGCATACATTAGCAACACTCCCAACTCACCCAGAAAGCGTCCCCATCAATGCACTGGTTTCAGTGAAAGGAACACCTCTTCAAGATCAAAAGCCAGTTGAAATCTGGGAGATGATACGGATGATTGCCACAGCACGTATAGTCATGCCAAATGCAATGGTCAGATTGTCAGCTGGCAGAGTTAAGTTCTCTATGCCCGAGCAGGCATTGTGTTTTCTTGCCGGGGCAAATTCAATATTCACTGGGGAGAAGCTATTGACTACACCGAACAACGATTTTGATGCTGATCAACTCATGTTCAAGATGCTTGGTTTGATTCCAAAAGCTCCTAGTTTCTCTGATGAACCAGCAAACGCAGCGTGTGAGGCAGCGGCTTGTGAGGAAGCTGTTTCCAGTTCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
BATS Radical_SAM

Protein Analysis

383

Amino Acids

41.91

Weight (kDa)

6.52

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 89 - 251 3.4e-23 Radical SAM superfamily
BATS PF06968 263 - 353 4.5e-27 Biotin and Thiamin Synthesis associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43360
fragaria_vesca FvH4_4g11460 FvH4_4g11460
malus_domestica MD04G1037300.v1.1
prunus_persica Prupe.1G186700_v2.0.a1
pyrus_communis pycom04g03040
rosa_chinensis RchiOBHm_Chr4g0408131
rosa_laevigata RLG00000008670 RLG00000008673
rosa_multiflora Rmu_sc0002073.1_g000002
rosa_roxburghii Rroxscaffold_5G00352620
rosa_rugosa Rorug04G0083700
rosa_samantha Rh4AG148100 Rh4BG145300 Rh4DG141800
rosa_wichuraiana Rw4G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
AatII GACGTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 168
AccII CGCG 1 cut(s) 150
AciI CCGC 3 cut(s) 47, 148, 1115
AclWI GGATC 2 cut(s) 460, 738
AcsI RAATTY 1 cut(s) 973
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 1 cut(s) 253
AfeI AGCGCT 1 cut(s) 647
AgsI TTSAA 7 cut(s) 104, 473, 550, 827, 845, 978, 1045
AjnI CCWGG 2 cut(s) 314, 425
AloI GAACNNNNNNTCC 2 cut(s) 195, 227
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 1 cut(s) 31
AlwI GGATC 2 cut(s) 460, 738
Ama87I CYCGRG 1 cut(s) 941
Aor51HI AGCGCT 1 cut(s) 647
AoxI GGCC 3 cut(s) 127, 336, 565
ApoI RAATTY 1 cut(s) 973
AseI ATTAAT 1 cut(s) 684
AspLEI GCGC 1 cut(s) 648
AspS9I GGNCC 5 cut(s) 15, 128, 144, 337, 633
AsuC2I CCSGG 2 cut(s) 671, 967
AsuHPI GGTGA 1 cut(s) 764
AvaI CYCGRG 1 cut(s) 941
AvaII GGWCC 3 cut(s) 15, 144, 633
AxyI CCTNAGG 1 cut(s) 520
BanII GRGCYC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 208
BccI CCATC 1 cut(s) 797
BceAI ACGGC 1 cut(s) 187
BcgI CGANNNNNNTGC 2 cut(s) 1007, 1041
BciT130I CCWGG 2 cut(s) 316, 427
BciVI GTATCC 1 cut(s) 427
BclI TGATCA 1 cut(s) 1030
BcnI CCSGG 2 cut(s) 671, 967
BcoDI GTCTC 1 cut(s) 31
BfaI CTAG 3 cut(s) 132, 600, 1074
BfmI CTRYAG 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 649
BfuI GTATCC 1 cut(s) 427
Bme1390I CCNGG 4 cut(s) 316, 427, 671, 967
Bme18I GGWCC 3 cut(s) 15, 144, 633
BmeT110I CYCGRG 1 cut(s) 941
BmgT120I GGNCC 5 cut(s) 15, 128, 144, 337, 633
BmiI GGNNCC 2 cut(s) 146, 175
BmrFI CCNGG 4 cut(s) 316, 427, 671, 967
BmrI ACTGGG 1 cut(s) 996
BmsI GCATC 4 cut(s) 349, 664, 1015, 1038
BmuI ACTGGG 1 cut(s) 996
BplI GAGNNNNNCTC 2 cut(s) 278, 310
BpmI CTGGAG 2 cut(s) 298, 448
BpuEI CTTGAG 2 cut(s) 192, 317
BpuMI CCSGG 2 cut(s) 671, 967
BsaAI YACGTR 1 cut(s) 881
BsaBI GATNNNNATC 1 cut(s) 1029
BsaHI GRCGYC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 966
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bse1I ACTGG 5 cut(s) 146, 804, 839, 991, 1137
Bse21I CCTNAGG 1 cut(s) 520
Bse3DI GCAATG 1 cut(s) 906
Bse8I GATNNNNATC 1 cut(s) 1029
BseBI CCWGG 2 cut(s) 316, 427
BseDI CCNNGG 1 cut(s) 966
BseGI GGATG 4 cut(s) 284, 364, 679, 870
BseJI GATNNNNATC 1 cut(s) 1029
BseMI GCAATG 1 cut(s) 906
BseMII CTCAG 2 cut(s) 26, 99
BseNI ACTGG 5 cut(s) 146, 804, 839, 991, 1137
BseRI GAGGAG 2 cut(s) 248, 740
BsgI GTGCAG 2 cut(s) 263, 499
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 3 cut(s) 129, 338, 567
BsiHKAI GWGCWC 1 cut(s) 208
BsiHKCI CYCGRG 1 cut(s) 941
BsiSI CCGG 2 cut(s) 670, 966
BslFI GGGAC 1 cut(s) 770
BsmAI GTCTC 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 770
BsmI GAATGC 1 cut(s) 61
BsnI GGCC 3 cut(s) 129, 338, 567
Bso31I GGTCTC 1 cut(s) 31
BsoBI CYCGRG 1 cut(s) 941
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 6 cut(s) 156, 187, 465, 730, 829, 1030
BspACI CCGC 3 cut(s) 47, 148, 1115
BspANI GGCC 3 cut(s) 129, 338, 567
BspCNI CTCAG 2 cut(s) 27, 100
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 2 cut(s) 146, 175
BspMAI CTGCAG 1 cut(s) 100
BspPI GGATC 2 cut(s) 460, 738
BspTNI GGTCTC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 906
BsrI ACTGG 5 cut(s) 146, 804, 839, 991, 1137
BssECI CCNNGG 1 cut(s) 966
BssMI GATC 6 cut(s) 156, 187, 465, 730, 829, 1030
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 316, 427
Bst4CI ACNGT 2 cut(s) 42, 408
Bst6I CTCTTC 2 cut(s) 82, 828
BstACI GRCGYC 1 cut(s) 165
BstBAI YACGTR 1 cut(s) 881
BstC8I GCNNGC 3 cut(s) 565, 919, 948
BstDEI CTNAG 3 cut(s) 35, 108, 520
BstF5I GGATG 4 cut(s) 284, 364, 679, 870
BstFNI CGCG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 649
BstHHI GCGC 1 cut(s) 648
BstKTI GATC 6 cut(s) 159, 190, 468, 733, 832, 1033
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 6 cut(s) 156, 187, 465, 730, 829, 1030
BstMWI GCNNNNNNNGC 7 cut(s) 31, 104, 383, 392, 522, 1100, 1109
BstNI CCWGG 2 cut(s) 316, 427
BstNSI RCATGY 1 cut(s) 668
BstSCI CCNGG 4 cut(s) 314, 425, 669, 965
BstSFI CTRYAG 1 cut(s) 96
BstUI CGCG 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 465
BstYI RGATCY 1 cut(s) 465
Bsu36I CCTNAGG 1 cut(s) 520
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 3 cut(s) 129, 338, 567
BtsCI GGATG 4 cut(s) 284, 364, 679, 870
BtsI GCAGTG 1 cut(s) 254
BtsIMutI CAGTG 4 cut(s) 254, 797, 813, 984
Cac8I GCNNGC 3 cut(s) 565, 919, 948
CfoI GCGC 1 cut(s) 648
Cfr13I GGNCC 5 cut(s) 15, 128, 144, 337, 633
CseI GACGC 1 cut(s) 772
Csp6I GTAC 1 cut(s) 252
CviAII CATG 6 cut(s) 367, 415, 526, 665, 889, 1039
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 3 cut(s) 35, 108, 520
DpnI GATC 6 cut(s) 158, 189, 467, 732, 831, 1032
DpnII GATC 6 cut(s) 156, 187, 465, 730, 829, 1030
Eam1104I CTCTTC 2 cut(s) 82, 828
EarI CTCTTC 2 cut(s) 82, 828
Ecl136II GAGCTC 1 cut(s) 206
Eco147I AGGCCT 1 cut(s) 567
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 1 cut(s) 31
Eco47I GGWCC 3 cut(s) 15, 144, 633
Eco47III AGCGCT 1 cut(s) 647
Eco53kI GAGCTC 1 cut(s) 206
Eco81I CCTNAGG 1 cut(s) 520
Eco88I CYCGRG 1 cut(s) 941
EcoICRI GAGCTC 1 cut(s) 206
EcoO109I RGGNCCY 2 cut(s) 128, 633
EcoRII CCWGG 2 cut(s) 314, 425
EcoT38I GRGCYC 1 cut(s) 208
FaeI CATG 6 cut(s) 370, 418, 529, 668, 892, 1042
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 1 cut(s) 770
FatI CATG 6 cut(s) 366, 414, 525, 664, 888, 1038
FauI CCCGC 1 cut(s) 155
FbaI TGATCA 1 cut(s) 1030
FblI GTMKAC 1 cut(s) 168
FokI GGATG 4 cut(s) 291, 371, 686, 877
FriOI GRGCYC 1 cut(s) 208
FspBI CTAG 3 cut(s) 132, 600, 1074
GlaI GCGC 1 cut(s) 647
GsuI CTGGAG 2 cut(s) 298, 448
HaeII RGCGCY 1 cut(s) 649
HaeIII GGCC 3 cut(s) 129, 338, 567
HapII CCGG 2 cut(s) 670, 966
HgaI GACGC 1 cut(s) 772
HhaI GCGC 1 cut(s) 648
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 6 cut(s) 370, 418, 529, 668, 892, 1042
Hin6I GCGC 1 cut(s) 646
HinP1I GCGC 1 cut(s) 646
HindIII AAGCTT 2 cut(s) 344, 543
HinfI GANTC 2 cut(s) 330, 1060
HpaII CCGG 2 cut(s) 670, 966
HphI GGTGA 1 cut(s) 764
Hpy166II GTNNAC 2 cut(s) 169, 252
Hpy188I TCNGA 2 cut(s) 908, 1084
Hpy188III TCNNGA 5 cut(s) 185, 590, 696, 827, 1045
Hpy8I GTNNAC 2 cut(s) 169, 252
Hpy99I CGWCG 3 cut(s) 46, 91, 155
HpyCH4III ACNGT 2 cut(s) 42, 408
HpyCH4IV ACGT 2 cut(s) 165, 880
HpyCH4V TGCA 9 cut(s) 61, 98, 244, 414, 516, 677, 748, 797, 899
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 104, 383, 392, 522, 1100, 1109
HpyF3I CTNAG 3 cut(s) 35, 108, 520
HpySE526I ACGT 2 cut(s) 165, 880
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 6 cut(s) 370, 418, 529, 668, 892, 1042
HspAI GCGC 1 cut(s) 646
Ksp22I TGATCA 1 cut(s) 1030
Kzo9I GATC 6 cut(s) 156, 187, 465, 730, 829, 1030
LmnI GCTCC 3 cut(s) 179, 203, 1075
LweI GCATC 4 cut(s) 349, 664, 1015, 1038
MaeI CTAG 3 cut(s) 132, 600, 1074
MaeII ACGT 2 cut(s) 165, 880
MalI GATC 6 cut(s) 158, 189, 467, 732, 831, 1032
MboI GATC 6 cut(s) 156, 187, 465, 730, 829, 1030
MboII GAAGA 4 cut(s) 57, 69, 456, 815
MflI RGATCY 1 cut(s) 465
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 3 cut(s) 229, 681, 973
MlyI GAGTC 1 cut(s) 324
MseI TTAA 4 cut(s) 459, 660, 684, 927
MspA1I CMGCKG 4 cut(s) 34, 95, 917, 1115
MspI CCGG 2 cut(s) 670, 966
MspR9I CCNGG 4 cut(s) 316, 427, 671, 967
Mva1269I GAATGC 1 cut(s) 61
MvaI CCWGG 2 cut(s) 316, 427
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 7 cut(s) 31, 104, 383, 392, 522, 1100, 1109
NciI CCSGG 2 cut(s) 671, 967
NdeII GATC 6 cut(s) 156, 187, 465, 730, 829, 1030
NlaIII CATG 6 cut(s) 370, 418, 529, 668, 892, 1042
NlaIV GGNNCC 2 cut(s) 146, 175
NspI RCATGY 1 cut(s) 668
PceI AGGCCT 1 cut(s) 567
PctI GAATGC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 1060
PfoI TCCNGGA 1 cut(s) 669
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
Ppu21I YACGTR 1 cut(s) 881
PpuMI RGGWCCY 1 cut(s) 633
PshBI ATTAAT 1 cut(s) 684
PsiI TTATAA 1 cut(s) 579
Psp124BI GAGCTC 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 633
Psp6I CCWGG 2 cut(s) 314, 425
PspGI CCWGG 2 cut(s) 314, 425
PspN4I GGNNCC 2 cut(s) 146, 175
PspPI GGNCC 5 cut(s) 15, 128, 144, 337, 633
PspPPI RGGWCCY 1 cut(s) 633
PstI CTGCAG 1 cut(s) 100
PsuI RGATCY 1 cut(s) 465
PvuII CAGCTG 3 cut(s) 34, 95, 917
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
SacI GAGCTC 1 cut(s) 208
SaqAI TTAA 4 cut(s) 459, 660, 684, 927
Sau3AI GATC 6 cut(s) 156, 187, 465, 730, 829, 1030
Sau96I GGNCC 5 cut(s) 15, 128, 144, 337, 633
SchI GAGTC 1 cut(s) 324
ScrFI CCNGG 4 cut(s) 316, 427, 671, 967
SduI GDGCHC 1 cut(s) 208
SfaNI GCATC 4 cut(s) 349, 664, 1015, 1038
SfcI CTRYAG 1 cut(s) 96
SinI GGWCC 3 cut(s) 15, 144, 633
SmlI CTYRAG 2 cut(s) 207, 332
SmoI CTYRAG 2 cut(s) 207, 332
Sse9I AATT 3 cut(s) 229, 681, 973
SseBI AGGCCT 1 cut(s) 567
SsiI CCGC 3 cut(s) 47, 148, 1115
SspI AATATT 2 cut(s) 608, 981
SspMI CTAG 3 cut(s) 132, 600, 1074
SstI GAGCTC 1 cut(s) 208
StuI AGGCCT 1 cut(s) 567
StyD4I CCNGG 4 cut(s) 314, 425, 669, 965
TaaI ACNGT 2 cut(s) 42, 408
TaiI ACGT 2 cut(s) 168, 883
TaqI TCGA 2 cut(s) 186, 733
TasI AATT 3 cut(s) 229, 681, 973
TatI WGTACW 1 cut(s) 251
TauI GCSGC 1 cut(s) 1118
TfiI GAWTC 1 cut(s) 1060
Tru1I TTAA 4 cut(s) 459, 660, 684, 927
Tru9I TTAA 4 cut(s) 459, 660, 684, 927
TscAI CASTG 4 cut(s) 254, 804, 813, 991
TspDTI ATGAA 3 cut(s) 57, 203, 1101
TspGWI ACGGA 3 cut(s) 7, 216, 877
TspRI CASTG 4 cut(s) 254, 804, 813, 991
VpaK11BI GGWCC 3 cut(s) 15, 144, 633
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 1 cut(s) 973
XceI RCATGY 1 cut(s) 668
XcmI CCANNNNNNNNNTGG 2 cut(s) 796, 846
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 3 cut(s) 132, 600, 1074
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.