RLG00000008673

Biotin synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015317
Physical Location & Seq
Reverse (-)
34712981 .. 34718964
5984 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008673

Sequence Viewer

Length: 1137 bp
ATGATGTTCTCCATAAGGTCCATTCTGCGCCAAGGCCTCAGACCGTCGTCGGCGGCATTTTACTCCTCTCTATCTTCATCAGCAGCTGCAATTGAAGCGGAGAGAGCAGTCCTACAAGGTCCTCGAAATGACTGGACTCGCGACGAGATCAGAGCCGTCTACGACTCTCCTGTTCTCGATCTTCTCTTCCATGGAGCTCAGGTTCACAGACATACACATAATTTTAGAGAAGTGCAACAGTGCACTCTCCTCTCTATCAAGACTGGTGGCTGTAGTGAGGATTGCTCCTATTGCCCTCAGTCCTCTAGATACAATACCGGACTCAAGGCGCAAAAGCTTATGAATAAGGACGATGTAATGAAAGCAGCGAAAAAGGCAAAAGAGGCAGGTAGCACACGCTTTTGCATGGGTGCTGCCTGGAGGGAGACAGTAGGAAGAAAGACAAACTTTAACCAGATCCTTGAATATGTGAAAGAAATAAGGAATTTGGGAATGGAAGTGTGTTGCACCTTAGGCATGATAGAACAGCAGCAAGCGTTAAAACTTAAGGAAGCTGGCCTGACAGCTTATAATCACAATCTAGACACCTCAAGGGAATATTATCCCAATGTCATTACCACAAGGACCTATGATGACCGCTTGGAAACAATTAAGTATGTCAGGGATGCAGGAATTAGTGTCTGTTCAGGAGGAATAATAGGGCTCGGAGAAGCAGAGGAGGATCGAGTTGGTTTGTTGCATACATTAGCAACACTTCCATCTCACCCAGAAAGTGTTCCCATAAACGCGCTGATTAGTGTGAAAGGGACACCTCTTCAGGATCAAAAGCCAGTTGAAATATGGGAGATGATACGCATGATTGCCACAGCACGTATAGTCATGCCAAAAGCCATGGTCAGACTGTCAGCTGGCAGAGTTCGTTTTTCAATGCCTGAGCAGGCACTGTGCTTTCTTGCTGGGGCAAATTCAATATTTACTGGTGAAAAACTATTGACCACACCTAACAATGATTTTGATGCCGATCAAGTTATGTTCAAGGTGCTTGGACTAATTCCAAAACCTCCCAGTTTCTCCGATGAACCAGCAAAGGCATCCGAGGCAGAAGCTTGTGAGGATGCTGTTTCCGTTTCAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
BATS Radical_SAM

Protein Analysis

379

Amino Acids

41.75

Weight (kDa)

7.03

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 86 - 248 1.7e-22 Radical SAM superfamily
BATS PF06968 260 - 350 6.5e-27 Biotin and Thiamin Synthesis associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43360
fragaria_vesca FvH4_4g11460 FvH4_4g11460
malus_domestica MD04G1037300.v1.1
prunus_persica Prupe.1G186700_v2.0.a1
pyrus_communis pycom04g03040
rosa_chinensis RchiOBHm_Chr4g0408131
rosa_laevigata RLG00000008670 RLG00000008673
rosa_multiflora Rmu_sc0002073.1_g000002
rosa_roxburghii Rroxscaffold_5G00352620
rosa_rugosa Rorug04G0083700
rosa_samantha Rh4AG148100 Rh4BG145300 Rh4DG141800
rosa_wichuraiana Rw4G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 570
Acc36I ACCTGC 1 cut(s) 377
AccI GTMKAC 1 cut(s) 159
AccII CGCG 2 cut(s) 141, 788
AciI CCGC 3 cut(s) 53, 98, 637
AclWI GGATC 3 cut(s) 451, 729, 828
AcsI RAATTY 2 cut(s) 484, 964
AcuI CTGAAG 1 cut(s) 800
AflII CTTAAG 1 cut(s) 545
AgsI TTSAA 6 cut(s) 95, 464, 836, 927, 969, 1036
AjnI CCWGG 1 cut(s) 416
AloI GAACNNNNNNTCC 2 cut(s) 186, 218
AluBI AGCT 7 cut(s) 86, 197, 337, 554, 566, 908, 1106
AluI AGCT 7 cut(s) 86, 197, 337, 554, 566, 908, 1106
Alw21I GWGCWC 2 cut(s) 199, 245
Alw26I GTCTC 1 cut(s) 419
Alw44I GTGCAC 1 cut(s) 241
AlwI GGATC 3 cut(s) 451, 729, 828
AlwNI CAGNNNCTG 2 cut(s) 86, 943
AoxI GGCC 2 cut(s) 34, 556
ApaLI GTGCAC 1 cut(s) 241
ApeKI GCWGC 5 cut(s) 83, 86, 365, 413, 529
ApoI RAATTY 2 cut(s) 484, 964
Asp700I GAANNNNTTC 1 cut(s) 774
AspLEI GCGC 3 cut(s) 30, 331, 790
AspS9I GGNCC 3 cut(s) 18, 119, 624
AsuHPI GGTGA 2 cut(s) 755, 992
AvaII GGWCC 3 cut(s) 18, 119, 624
AxyI CCTNAGG 1 cut(s) 511
BaeGI GKGCMC 1 cut(s) 245
BanII GRGCYC 2 cut(s) 199, 705
Bbv12I GWGCWC 2 cut(s) 199, 245
BbvI GCAGC 5 cut(s) 73, 95, 377, 400, 541
BccI CCATC 1 cut(s) 766
BceAI ACGGC 1 cut(s) 140
BciT130I CCWGG 1 cut(s) 418
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 2 cut(s) 306, 581
BfmI CTRYAG 1 cut(s) 271
BfrI CTTAAG 1 cut(s) 545
BfuAI ACCTGC 1 cut(s) 377
BisI GCNGC 6 cut(s) 54, 84, 87, 366, 414, 530
BlsI GCNGC 6 cut(s) 55, 85, 88, 367, 415, 531
Bme1390I CCNGG 1 cut(s) 418
Bme18I GGWCC 3 cut(s) 18, 119, 624
BmgT120I GGNCC 3 cut(s) 18, 119, 624
BmrFI CCNGG 1 cut(s) 418
BmrI ACTGGG 1 cut(s) 1059
BmsI GCATC 4 cut(s) 655, 1006, 1100, 1105
BmuI ACTGGG 1 cut(s) 1059
BoxI GACNNNNGTC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 269, 301
BpmI CTGGAG 1 cut(s) 439
Bpu10I CCTNAGC 2 cut(s) 198, 933
BpuEI CTTGAG 2 cut(s) 308, 574
BsaAI YACGTR 1 cut(s) 872
BsaBI GATNNNNATC 1 cut(s) 1020
BsaJI CCNNGG 4 cut(s) 31, 190, 891, 1095
BsaWI WCCGGW 1 cut(s) 317
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bse1I ACTGG 5 cut(s) 137, 268, 830, 982, 1065
Bse21I CCTNAGG 1 cut(s) 511
Bse8I GATNNNNATC 1 cut(s) 1020
BseBI CCWGG 1 cut(s) 418
BseDI CCNNGG 4 cut(s) 31, 190, 891, 1095
BseGI GGATG 3 cut(s) 670, 1091, 1120
BseJI GATNNNNATC 1 cut(s) 1020
BseMII CTCAG 4 cut(s) 52, 212, 311, 924
BseNI ACTGG 5 cut(s) 137, 268, 830, 982, 1065
BseRI GAGGAG 3 cut(s) 55, 239, 731
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 5 cut(s) 73, 95, 377, 400, 541
BseYI CCCAGC 1 cut(s) 956
Bsh1236I CGCG 2 cut(s) 141, 788
BshFI GGCC 2 cut(s) 36, 558
BsiHKAI GWGCWC 2 cut(s) 199, 245
BsiSI CCGG 1 cut(s) 318
BslFI GGGAC 1 cut(s) 820
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 820
BsnI GGCC 2 cut(s) 36, 558
Bsp1286I GDGCHC 3 cut(s) 199, 245, 705
Bsp143I GATC 6 cut(s) 147, 178, 456, 721, 820, 1021
Bsp19I CCATGG 2 cut(s) 190, 891
Bsp68I TCGCGA 1 cut(s) 141
BspACI CCGC 3 cut(s) 53, 98, 637
BspANI GGCC 2 cut(s) 36, 558
BspCNI CTCAG 4 cut(s) 51, 211, 310, 925
BspFNI CGCG 2 cut(s) 141, 788
BspMI ACCTGC 1 cut(s) 377
BspPI GGATC 3 cut(s) 451, 729, 828
BspTI CTTAAG 1 cut(s) 545
BsrI ACTGG 5 cut(s) 137, 268, 830, 982, 1065
BssECI CCNNGG 4 cut(s) 31, 190, 891, 1095
BssMI GATC 6 cut(s) 147, 178, 456, 721, 820, 1021
BssT1I CCWWGG 3 cut(s) 31, 190, 891
Bst2UI CCWGG 1 cut(s) 418
Bst4CI ACNGT 5 cut(s) 45, 240, 430, 903, 945
Bst6I CTCTTC 2 cut(s) 191, 819
BstAFI CTTAAG 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 872
BstC8I GCNNGC 4 cut(s) 534, 556, 910, 939
BstDEI CTNAG 5 cut(s) 38, 198, 297, 511, 933
BstDSI CCRYGG 2 cut(s) 190, 891
BstF5I GGATG 3 cut(s) 670, 1091, 1120
BstFNI CGCG 2 cut(s) 141, 788
BstHHI GCGC 3 cut(s) 30, 331, 790
BstKTI GATC 6 cut(s) 150, 181, 459, 724, 823, 1024
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 6 cut(s) 147, 178, 456, 721, 820, 1021
BstMWI GCNNNNNNNGC 7 cut(s) 95, 104, 291, 374, 383, 513, 1097
BstNI CCWGG 1 cut(s) 418
BstPAI GACNNNNGTC 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 416
BstSFI CTRYAG 1 cut(s) 271
BstSLI GKGCMC 1 cut(s) 245
BstUI CGCG 2 cut(s) 141, 788
BstV1I GCAGC 5 cut(s) 73, 95, 377, 400, 541
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
Bsu36I CCTNAGG 1 cut(s) 511
BsuRI GGCC 2 cut(s) 36, 558
BtgI CCRYGG 2 cut(s) 190, 891
BtsCI GGATG 3 cut(s) 670, 1091, 1120
BtsIMutI CAGTG 2 cut(s) 245, 941
BtuMI TCGCGA 1 cut(s) 141
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 4 cut(s) 534, 556, 910, 939
CaiI CAGNNNCTG 2 cut(s) 86, 943
CfoI GCGC 3 cut(s) 30, 331, 790
Cfr13I GGNCC 3 cut(s) 18, 119, 624
CspCI CAANNNNNGTGG 2 cut(s) 247, 282
CviAII CATG 6 cut(s) 191, 406, 517, 856, 880, 892
DdeI CTNAG 5 cut(s) 38, 198, 297, 511, 933
DpnI GATC 6 cut(s) 149, 180, 458, 723, 822, 1023
DpnII GATC 6 cut(s) 147, 178, 456, 721, 820, 1021
Eam1104I CTCTTC 2 cut(s) 191, 819
EarI CTCTTC 2 cut(s) 191, 819
Ecl136II GAGCTC 1 cut(s) 197
Eco130I CCWWGG 3 cut(s) 31, 190, 891
Eco147I AGGCCT 1 cut(s) 36
Eco24I GRGCYC 2 cut(s) 199, 705
Eco47I GGWCC 3 cut(s) 18, 119, 624
Eco53kI GAGCTC 1 cut(s) 197
Eco57I CTGAAG 1 cut(s) 800
Eco81I CCTNAGG 1 cut(s) 511
EcoICRI GAGCTC 1 cut(s) 197
EcoO109I RGGNCCY 2 cut(s) 119, 624
EcoRII CCWGG 1 cut(s) 416
EcoT14I CCWWGG 3 cut(s) 31, 190, 891
EcoT38I GRGCYC 2 cut(s) 199, 705
ErhI CCWWGG 3 cut(s) 31, 190, 891
FaeI CATG 6 cut(s) 194, 409, 520, 859, 883, 895
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FaqI GGGAC 1 cut(s) 820
FatI CATG 6 cut(s) 190, 405, 516, 855, 879, 891
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 6 cut(s) 54, 84, 87, 366, 414, 530
FokI GGATG 3 cut(s) 677, 1078, 1127
FriOI GRGCYC 2 cut(s) 199, 705
Fsp4HI GCNGC 6 cut(s) 54, 84, 87, 366, 414, 530
FspBI CTAG 2 cut(s) 306, 581
GlaI GCGC 3 cut(s) 29, 330, 789
GluI GCNGC 6 cut(s) 54, 84, 87, 366, 414, 530
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 2 cut(s) 36, 558
HapII CCGG 1 cut(s) 318
HhaI GCGC 3 cut(s) 30, 331, 790
Hin1II CATG 6 cut(s) 194, 409, 520, 859, 883, 895
Hin6I GCGC 3 cut(s) 28, 329, 788
HinP1I GCGC 3 cut(s) 28, 329, 788
HindIII AAGCTT 2 cut(s) 335, 1104
HinfI GANTC 3 cut(s) 136, 164, 321
HpaII CCGG 1 cut(s) 318
HphI GGTGA 2 cut(s) 755, 992
Hpy166II GTNNAC 3 cut(s) 160, 205, 243
Hpy188I TCNGA 6 cut(s) 41, 152, 707, 899, 1075, 1096
Hpy188III TCNNGA 7 cut(s) 140, 176, 259, 306, 581, 687, 818
Hpy8I GTNNAC 3 cut(s) 160, 205, 243
Hpy99I CGWCG 3 cut(s) 49, 52, 146
HpyCH4III ACNGT 5 cut(s) 45, 240, 430, 903, 945
HpyCH4IV ACGT 1 cut(s) 871
HpyCH4V TGCA 7 cut(s) 89, 235, 243, 405, 507, 668, 739
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 104, 291, 374, 383, 513, 1097
HpyF3I CTNAG 5 cut(s) 38, 198, 297, 511, 933
HpySE526I ACGT 1 cut(s) 871
Hsp92II CATG 6 cut(s) 194, 409, 520, 859, 883, 895
HspAI GCGC 3 cut(s) 28, 329, 788
Kzo9I GATC 6 cut(s) 147, 178, 456, 721, 820, 1021
LmnI GCTCC 2 cut(s) 194, 290
Lsp1109I GCAGC 5 cut(s) 73, 95, 377, 400, 541
LweI GCATC 4 cut(s) 655, 1006, 1100, 1105
MaeI CTAG 2 cut(s) 306, 581
MaeII ACGT 1 cut(s) 871
MalI GATC 6 cut(s) 149, 180, 458, 723, 822, 1023
MboI GATC 6 cut(s) 147, 178, 456, 721, 820, 1021
MboII GAAGA 5 cut(s) 66, 173, 178, 447, 806
MfeI CAATTG 1 cut(s) 90
MflI RGATCY 1 cut(s) 456
MhlI GDGCHC 3 cut(s) 199, 245, 705
MluCI AATT 7 cut(s) 90, 220, 484, 648, 672, 964, 1050
MlyI GAGTC 3 cut(s) 130, 158, 315
MroXI GAANNNNTTC 1 cut(s) 774
MseI TTAA 4 cut(s) 450, 539, 546, 651
MspA1I CMGCKG 2 cut(s) 86, 908
MspCI CTTAAG 1 cut(s) 545
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 1 cut(s) 418
MunI CAATTG 1 cut(s) 90
MvaI CCWGG 1 cut(s) 418
MvnI CGCG 2 cut(s) 141, 788
MwoI GCNNNNNNNGC 7 cut(s) 95, 104, 291, 374, 383, 513, 1097
NcoI CCATGG 2 cut(s) 190, 891
NdeII GATC 6 cut(s) 147, 178, 456, 721, 820, 1021
NlaIII CATG 6 cut(s) 194, 409, 520, 859, 883, 895
NruI TCGCGA 1 cut(s) 141
PceI AGGCCT 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 774
PkrI GCNGC 6 cut(s) 55, 85, 88, 367, 415, 531
PleI GAGTC 3 cut(s) 130, 158, 315
PpsI GAGTC 3 cut(s) 130, 158, 315
Ppu21I YACGTR 1 cut(s) 872
PpuMI RGGWCCY 2 cut(s) 119, 624
PshAI GACNNNNGTC 1 cut(s) 46
PsiI TTATAA 1 cut(s) 570
Psp124BI GAGCTC 1 cut(s) 199
Psp5II RGGWCCY 2 cut(s) 119, 624
Psp6I CCWGG 1 cut(s) 416
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 1 cut(s) 416
PspPI GGNCC 3 cut(s) 18, 119, 624
PspPPI RGGWCCY 2 cut(s) 119, 624
PstNI CAGNNNCTG 2 cut(s) 86, 943
PsuI RGATCY 1 cut(s) 456
PvuII CAGCTG 2 cut(s) 86, 908
RruI TCGCGA 1 cut(s) 141
SacI GAGCTC 1 cut(s) 199
SaqAI TTAA 4 cut(s) 450, 539, 546, 651
SatI GCNGC 6 cut(s) 54, 84, 87, 366, 414, 530
Sau3AI GATC 6 cut(s) 147, 178, 456, 721, 820, 1021
Sau96I GGNCC 3 cut(s) 18, 119, 624
SchI GAGTC 3 cut(s) 130, 158, 315
ScrFI CCNGG 1 cut(s) 418
SduI GDGCHC 3 cut(s) 199, 245, 705
SfaNI GCATC 4 cut(s) 655, 1006, 1100, 1105
SfcI CTRYAG 1 cut(s) 271
SinI GGWCC 3 cut(s) 18, 119, 624
SmlI CTYRAG 3 cut(s) 323, 545, 589
SmoI CTYRAG 3 cut(s) 323, 545, 589
Sse9I AATT 7 cut(s) 90, 220, 484, 648, 672, 964, 1050
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 3 cut(s) 53, 98, 637
SspI AATATT 2 cut(s) 599, 972
SspMI CTAG 2 cut(s) 306, 581
SstI GAGCTC 1 cut(s) 199
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 416
StyI CCWWGG 3 cut(s) 31, 190, 891
TaaI ACNGT 5 cut(s) 45, 240, 430, 903, 945
TaiI ACGT 1 cut(s) 874
TaqI TCGA 3 cut(s) 124, 177, 724
TasI AATT 7 cut(s) 90, 220, 484, 648, 672, 964, 1050
TauI GCSGC 1 cut(s) 56
Tru1I TTAA 4 cut(s) 450, 539, 546, 651
Tru9I TTAA 4 cut(s) 450, 539, 546, 651
TscAI CASTG 2 cut(s) 245, 948
TseI GCWGC 5 cut(s) 83, 86, 365, 413, 529
TspDTI ATGAA 4 cut(s) 66, 356, 374, 1092
TspGWI ACGGA 1 cut(s) 1114
TspRI CASTG 2 cut(s) 245, 948
Vha464I CTTAAG 1 cut(s) 545
VneI GTGCAC 1 cut(s) 241
VpaK11BI GGWCC 3 cut(s) 18, 119, 624
XapI RAATTY 2 cut(s) 484, 964
XbaI TCTAGA 2 cut(s) 305, 580
XcmI CCANNNNNNNNNTGG 1 cut(s) 837
XmiI GTMKAC 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 774
XspI CTAG 2 cut(s) 306, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.