Rh4BG145300

Biotin synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015317
Physical Location & Seq
Forward (+)
25996790 .. 26002699
5910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG145300.1

Sequence Viewer

Length: 1158 bp
ATGATGTTCTCCATTAGGTACATTCTGCGCCAAGGCCTCAGACCCTCGTCGTCGTCGTCGGCGGCGGCGGCGGCGGCATTTTACTCCTCTCTATCTTCATCTGCAGCTGCAATTGAAGCGGAGAGAACAGTCCTGCAAGGCCCCCGAAATGACTGGACTCGTGACGAGATCAGAGCCGTCTACGACTCTCCTGTTCTCGATCTCCTCTTCCATGGAGCTCAGGTTCACAGACATACGCATAATTTTAGAGAAGTGCAACAGTGCACTCTCCTCTCTATCAAGACTGGTGGCTGTAGTGAGGACTGCTCCTATTGCCCTCAGTCCTCTAGATACGATACCGGACTCAAGGCACAAAAGCTTATGACTAAGGACGATGTTATGAAAGCAGCTAAAAAGGCAAAAGAGGCTGGTAGCACACGCTTTTGCATGGGTGCTGCCTGGAGGGAGACAGTAGGAAGAAAGACAAACTTCAACCAGATCCTTGAATATGTGAAAGAAATAAGGAATTTGGGAATGGAAGTGTGCTGCACCTTAGGCATGATAGAACAGCAGCAAGCGTTAAAACTTAAGGAAGCTGGCCTGACAGCTTATAATCACAATCTAGACACCTCAAGGGAATATTATCCCAATGTCATTACCACAAGGACCTATGATGACCGCTTGGAAACAATTAAGTATGTCAGGGATGCAGGAATTAGTGTGTGTTCAGGAGGAATAATAGGGCTCGGAGAAGCAGAGGAGGATCGAGTTGGTTTGTTGCATACATTAGCAACACTTCCATCTCACCCAGAAAGTGTTCCCATAAACGCGCTGATTAGTGTGAAAGGGACACCTCTTCAGGATCAAAAGCCAGTTGAAATATGGGAGATGATACGCATGATTGCCACAGCACGTATAGTCATGCCAAAAGCCATGGTCAGACTGTCAGCTGGCAGAGTTCGTTTTTCAATGCCTGAGCAGGCACTGTGCTTTCTTGCTGGGGCAAATTCAATATTTACTGGTGAAAAACTATTGACCACACCTAACAATGATTTTGATGCCGATCAAGTTATGTTCAAGGTGCTTGGACTAATTCCAAAACCTCCAAGTTTCTCTGATGAATCAGCAAAGGCATCCGAGGCAGAAGCTTGTGAGGATGCTGTTTCCGTTTCAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
KEGG Pathways
Metabolic & Signaling
ko00780 ko01100 map00780 map01100
Pfam Domains
Protein Families
BATS Radical_SAM

Protein Analysis

385

Amino Acids

42.38

Weight (kDa)

6.67

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 93 - 255 4.4e-23 Radical SAM superfamily
BATS PF06968 267 - 357 6.7e-27 Biotin and Thiamin Synthesis associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015317)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43360
fragaria_vesca FvH4_4g11460 FvH4_4g11460
malus_domestica MD04G1037300.v1.1
prunus_persica Prupe.1G186700_v2.0.a1
pyrus_communis pycom04g03040
rosa_chinensis RchiOBHm_Chr4g0408131
rosa_laevigata RLG00000008670 RLG00000008673
rosa_multiflora Rmu_sc0002073.1_g000002
rosa_roxburghii Rroxscaffold_5G00352620
rosa_rugosa Rorug04G0083700
rosa_samantha Rh4AG148100 Rh4BG145300 Rh4DG141800
rosa_wichuraiana Rw4G012120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
AccI GTMKAC 1 cut(s) 180
AccII CGCG 1 cut(s) 809
AciI CCGC 7 cut(s) 62, 65, 68, 71, 74, 119, 658
AclWI GGATC 3 cut(s) 472, 750, 849
AcsI RAATTY 2 cut(s) 505, 985
AcuI CTGAAG 1 cut(s) 821
AfaI GTAC 1 cut(s) 20
AflII CTTAAG 1 cut(s) 566
AgsI TTSAA 7 cut(s) 116, 472, 485, 857, 948, 990, 1057
AjnI CCWGG 1 cut(s) 437
AloI GAACNNNNNNTCC 2 cut(s) 207, 239
AluBI AGCT 8 cut(s) 107, 218, 358, 389, 575, 587, 929, 1127
AluI AGCT 8 cut(s) 107, 218, 358, 389, 575, 587, 929, 1127
Alw21I GWGCWC 2 cut(s) 220, 266
Alw26I GTCTC 1 cut(s) 440
Alw44I GTGCAC 1 cut(s) 262
AlwI GGATC 3 cut(s) 472, 750, 849
AlwNI CAGNNNCTG 1 cut(s) 964
AoxI GGCC 3 cut(s) 34, 139, 577
ApaLI GTGCAC 1 cut(s) 262
ApeKI GCWGC 6 cut(s) 104, 107, 386, 434, 525, 550
ApoI RAATTY 2 cut(s) 505, 985
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 2 cut(s) 30, 811
AspS9I GGNCC 2 cut(s) 140, 645
AsuHPI GGTGA 2 cut(s) 776, 1013
AvaII GGWCC 1 cut(s) 645
AxyI CCTNAGG 1 cut(s) 532
BaeGI GKGCMC 1 cut(s) 266
BanII GRGCYC 2 cut(s) 220, 726
BauI CACGAG 1 cut(s) 159
Bbv12I GWGCWC 2 cut(s) 220, 266
BbvI GCAGC 6 cut(s) 94, 116, 398, 421, 512, 562
BccI CCATC 1 cut(s) 787
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 440
BfaI CTAG 2 cut(s) 327, 602
BfmI CTRYAG 2 cut(s) 102, 292
BfrI CTTAAG 1 cut(s) 566
Bme1390I CCNGG 1 cut(s) 439
Bme18I GGWCC 1 cut(s) 645
BmgT120I GGNCC 2 cut(s) 140, 645
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 1 cut(s) 439
BmsI GCATC 4 cut(s) 676, 1027, 1121, 1126
BoxI GACNNNNGTC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 1 cut(s) 460
Bpu10I CCTNAGC 2 cut(s) 219, 954
BpuEI CTTGAG 2 cut(s) 329, 595
BsaAI YACGTR 1 cut(s) 893
BsaBI GATNNNNATC 1 cut(s) 1041
BsaJI CCNNGG 4 cut(s) 31, 211, 912, 1116
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bse1I ACTGG 4 cut(s) 158, 289, 851, 1003
Bse21I CCTNAGG 1 cut(s) 532
Bse8I GATNNNNATC 1 cut(s) 1041
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 4 cut(s) 31, 211, 912, 1116
BseGI GGATG 3 cut(s) 691, 1112, 1141
BseJI GATNNNNATC 1 cut(s) 1041
BseMII CTCAG 4 cut(s) 52, 233, 332, 945
BseNI ACTGG 4 cut(s) 158, 289, 851, 1003
BseRI GAGGAG 4 cut(s) 76, 194, 260, 752
BseSI GKGCMC 1 cut(s) 266
BseXI GCAGC 6 cut(s) 94, 116, 398, 421, 512, 562
BseYI CCCAGC 1 cut(s) 977
BsgI GTGCAG 1 cut(s) 511
Bsh1236I CGCG 1 cut(s) 809
BshFI GGCC 3 cut(s) 36, 141, 579
BsiHKAI GWGCWC 2 cut(s) 220, 266
BsiSI CCGG 1 cut(s) 339
BslFI GGGAC 1 cut(s) 841
BsmAI GTCTC 1 cut(s) 440
BsmFI GGGAC 1 cut(s) 841
BsnI GGCC 3 cut(s) 36, 141, 579
Bsp1286I GDGCHC 3 cut(s) 220, 266, 726
Bsp143I GATC 6 cut(s) 168, 199, 477, 742, 841, 1042
Bsp19I CCATGG 2 cut(s) 211, 912
BspACI CCGC 7 cut(s) 62, 65, 68, 71, 74, 119, 658
BspANI GGCC 3 cut(s) 36, 141, 579
BspCNI CTCAG 4 cut(s) 51, 232, 331, 946
BspFNI CGCG 1 cut(s) 809
BspLI GGNNCC 1 cut(s) 142
BspMAI CTGCAG 1 cut(s) 106
BspPI GGATC 3 cut(s) 472, 750, 849
BspTI CTTAAG 1 cut(s) 566
BsrI ACTGG 4 cut(s) 158, 289, 851, 1003
BssECI CCNNGG 4 cut(s) 31, 211, 912, 1116
BssMI GATC 6 cut(s) 168, 199, 477, 742, 841, 1042
BssSI CACGAG 1 cut(s) 159
BssT1I CCWWGG 3 cut(s) 31, 211, 912
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 439
Bst4CI ACNGT 5 cut(s) 130, 261, 451, 924, 966
Bst6I CTCTTC 2 cut(s) 212, 840
BstAFI CTTAAG 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 893
BstC8I GCNNGC 4 cut(s) 555, 577, 931, 960
BstDEI CTNAG 6 cut(s) 38, 219, 318, 366, 532, 954
BstDSI CCRYGG 2 cut(s) 211, 912
BstF5I GGATG 3 cut(s) 691, 1112, 1141
BstFNI CGCG 1 cut(s) 809
BstHHI GCGC 2 cut(s) 30, 811
BstKTI GATC 6 cut(s) 171, 202, 480, 745, 844, 1045
BstMAI GTCTC 1 cut(s) 440
BstMBI GATC 6 cut(s) 168, 199, 477, 742, 841, 1042
BstMWI GCNNNNNNNGC 9 cut(s) 68, 71, 74, 116, 312, 395, 404, 534, 1118
BstNI CCWGG 1 cut(s) 439
BstPAI GACNNNNGTC 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 437
BstSFI CTRYAG 2 cut(s) 102, 292
BstSLI GKGCMC 1 cut(s) 266
BstUI CGCG 1 cut(s) 809
BstV1I GCAGC 6 cut(s) 94, 116, 398, 421, 512, 562
BstX2I RGATCY 1 cut(s) 477
BstYI RGATCY 1 cut(s) 477
Bsu36I CCTNAGG 1 cut(s) 532
BsuRI GGCC 3 cut(s) 36, 141, 579
BtgI CCRYGG 2 cut(s) 211, 912
BtsCI GGATG 3 cut(s) 691, 1112, 1141
BtsIMutI CAGTG 2 cut(s) 266, 962
Cac8I GCNNGC 4 cut(s) 555, 577, 931, 960
CaiI CAGNNNCTG 1 cut(s) 964
CfoI GCGC 2 cut(s) 30, 811
Cfr13I GGNCC 2 cut(s) 140, 645
Csp6I GTAC 1 cut(s) 19
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 6 cut(s) 212, 427, 538, 877, 901, 913
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 6 cut(s) 38, 219, 318, 366, 532, 954
DpnI GATC 6 cut(s) 170, 201, 479, 744, 843, 1044
DpnII GATC 6 cut(s) 168, 199, 477, 742, 841, 1042
Eam1104I CTCTTC 2 cut(s) 212, 840
EarI CTCTTC 2 cut(s) 212, 840
Ecl136II GAGCTC 1 cut(s) 218
Eco130I CCWWGG 3 cut(s) 31, 211, 912
Eco147I AGGCCT 1 cut(s) 36
Eco24I GRGCYC 2 cut(s) 220, 726
Eco47I GGWCC 1 cut(s) 645
Eco53kI GAGCTC 1 cut(s) 218
Eco57I CTGAAG 1 cut(s) 821
Eco81I CCTNAGG 1 cut(s) 532
EcoICRI GAGCTC 1 cut(s) 218
EcoO109I RGGNCCY 2 cut(s) 140, 645
EcoRII CCWGG 1 cut(s) 437
EcoT14I CCWWGG 3 cut(s) 31, 211, 912
EcoT38I GRGCYC 2 cut(s) 220, 726
ErhI CCWWGG 3 cut(s) 31, 211, 912
FaeI CATG 6 cut(s) 215, 430, 541, 880, 904, 916
FalI AAGNNNNNCTT 2 cut(s) 452, 484
FaqI GGGAC 1 cut(s) 841
FatI CATG 6 cut(s) 211, 426, 537, 876, 900, 912
FblI GTMKAC 1 cut(s) 180
FokI GGATG 3 cut(s) 698, 1099, 1148
FriOI GRGCYC 2 cut(s) 220, 726
FspBI CTAG 2 cut(s) 327, 602
GlaI GCGC 2 cut(s) 29, 810
GsaI CCCAGC 1 cut(s) 981
GsuI CTGGAG 1 cut(s) 460
HaeIII GGCC 3 cut(s) 36, 141, 579
HapII CCGG 1 cut(s) 339
HhaI GCGC 2 cut(s) 30, 811
Hin1II CATG 6 cut(s) 215, 430, 541, 880, 904, 916
Hin6I GCGC 2 cut(s) 28, 809
HinP1I GCGC 2 cut(s) 28, 809
HindIII AAGCTT 2 cut(s) 356, 1125
HinfI GANTC 4 cut(s) 157, 185, 342, 1100
HpaII CCGG 1 cut(s) 339
HphI GGTGA 2 cut(s) 776, 1013
Hpy166II GTNNAC 3 cut(s) 181, 226, 264
Hpy188I TCNGA 6 cut(s) 41, 173, 728, 920, 1096, 1117
Hpy188III TCNNGA 7 cut(s) 161, 197, 280, 327, 602, 708, 839
Hpy8I GTNNAC 3 cut(s) 181, 226, 264
Hpy99I CGWCG 4 cut(s) 52, 55, 58, 61
HpyCH4III ACNGT 5 cut(s) 130, 261, 451, 924, 966
HpyCH4IV ACGT 1 cut(s) 892
HpyCH4V TGCA 9 cut(s) 104, 110, 136, 256, 264, 426, 528, 689, 760
HpyF10VI GCNNNNNNNGC 9 cut(s) 68, 71, 74, 116, 312, 395, 404, 534, 1118
HpyF3I CTNAG 6 cut(s) 38, 219, 318, 366, 532, 954
HpySE526I ACGT 1 cut(s) 892
Hsp92II CATG 6 cut(s) 215, 430, 541, 880, 904, 916
HspAI GCGC 2 cut(s) 28, 809
Kzo9I GATC 6 cut(s) 168, 199, 477, 742, 841, 1042
LmnI GCTCC 2 cut(s) 215, 311
Lsp1109I GCAGC 6 cut(s) 94, 116, 398, 421, 512, 562
LweI GCATC 4 cut(s) 676, 1027, 1121, 1126
MaeI CTAG 2 cut(s) 327, 602
MaeII ACGT 1 cut(s) 892
MaeIII GTNAC 1 cut(s) 161
MalI GATC 6 cut(s) 170, 201, 479, 744, 843, 1044
MboI GATC 6 cut(s) 168, 199, 477, 742, 841, 1042
MboII GAAGA 4 cut(s) 87, 199, 468, 827
MfeI CAATTG 1 cut(s) 111
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 3 cut(s) 220, 266, 726
MluCI AATT 7 cut(s) 111, 241, 505, 669, 693, 985, 1071
MlyI GAGTC 3 cut(s) 151, 179, 336
MroXI GAANNNNTTC 1 cut(s) 795
MseI TTAA 3 cut(s) 560, 567, 672
MspA1I CMGCKG 2 cut(s) 107, 929
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 1 cut(s) 439
MunI CAATTG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 439
MvnI CGCG 1 cut(s) 809
MwoI GCNNNNNNNGC 9 cut(s) 68, 71, 74, 116, 312, 395, 404, 534, 1118
NcoI CCATGG 2 cut(s) 211, 912
NdeII GATC 6 cut(s) 168, 199, 477, 742, 841, 1042
NlaIII CATG 6 cut(s) 215, 430, 541, 880, 904, 916
NlaIV GGNNCC 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 161
PceI AGGCCT 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 1 cut(s) 1100
PleI GAGTC 3 cut(s) 151, 179, 336
PpsI GAGTC 3 cut(s) 151, 179, 336
Ppu21I YACGTR 1 cut(s) 893
PpuMI RGGWCCY 1 cut(s) 645
PshAI GACNNNNGTC 1 cut(s) 46
PsiI TTATAA 1 cut(s) 591
Psp124BI GAGCTC 1 cut(s) 220
Psp5II RGGWCCY 1 cut(s) 645
Psp6I CCWGG 1 cut(s) 437
PspFI CCCAGC 1 cut(s) 977
PspGI CCWGG 1 cut(s) 437
PspN4I GGNNCC 1 cut(s) 142
PspPI GGNCC 2 cut(s) 140, 645
PspPPI RGGWCCY 1 cut(s) 645
PstI CTGCAG 1 cut(s) 106
PstNI CAGNNNCTG 1 cut(s) 964
PsuI RGATCY 1 cut(s) 477
PvuII CAGCTG 2 cut(s) 107, 929
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
SacI GAGCTC 1 cut(s) 220
SaqAI TTAA 3 cut(s) 560, 567, 672
Sau3AI GATC 6 cut(s) 168, 199, 477, 742, 841, 1042
Sau96I GGNCC 2 cut(s) 140, 645
SchI GAGTC 3 cut(s) 151, 179, 336
ScrFI CCNGG 1 cut(s) 439
SduI GDGCHC 3 cut(s) 220, 266, 726
SfaNI GCATC 4 cut(s) 676, 1027, 1121, 1126
SfcI CTRYAG 2 cut(s) 102, 292
SinI GGWCC 1 cut(s) 645
SmlI CTYRAG 3 cut(s) 344, 566, 610
SmoI CTYRAG 3 cut(s) 344, 566, 610
Sse9I AATT 7 cut(s) 111, 241, 505, 669, 693, 985, 1071
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 7 cut(s) 62, 65, 68, 71, 74, 119, 658
SspI AATATT 2 cut(s) 620, 993
SspMI CTAG 2 cut(s) 327, 602
SstI GAGCTC 1 cut(s) 220
StuI AGGCCT 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 437
StyI CCWWGG 3 cut(s) 31, 211, 912
TaaI ACNGT 5 cut(s) 130, 261, 451, 924, 966
TaiI ACGT 1 cut(s) 895
TaqI TCGA 2 cut(s) 198, 745
TasI AATT 7 cut(s) 111, 241, 505, 669, 693, 985, 1071
TauI GCSGC 5 cut(s) 65, 68, 71, 74, 77
TfiI GAWTC 1 cut(s) 1100
Tru1I TTAA 3 cut(s) 560, 567, 672
Tru9I TTAA 3 cut(s) 560, 567, 672
TscAI CASTG 2 cut(s) 266, 969
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 6 cut(s) 104, 107, 386, 434, 525, 550
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 3 cut(s) 87, 395, 1113
TspGWI ACGGA 1 cut(s) 1135
TspRI CASTG 2 cut(s) 266, 969
Vha464I CTTAAG 1 cut(s) 566
VneI GTGCAC 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 645
XapI RAATTY 2 cut(s) 505, 985
XbaI TCTAGA 2 cut(s) 326, 601
XcmI CCANNNNNNNNNTGG 1 cut(s) 858
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 2 cut(s) 327, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.