FvH4_4g14070

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009246
Physical Location & Seq
Forward (+)
17593916 .. 17597960
4045 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g14070.t5

Sequence Viewer

Length: 1038 bp
ATGTCGTTCTCGTTCTTCAAGCCGTCGAGGCCCAAAACCCCACAGGAGGTCGCCAAGGCCATCAGCGACAGCCTCTCTGCCCTCGACTCCCAGACCGTCGCCGAAGTCAAATCCCTCGAAAAGGCGTTGGAAGAGGTGGAAAAGAATTTCGGGATTATGAGATGTATGCTTGTCGGAGATGGGGAGACGGAAGCTAATACGGAACAAGTTTCACAGCTTGTGTTCGAGATATGCAAGGAGGATGTTCTTGCTCTTCTAGTTCACAAGTTACCAATACTGGGATGGGAAGCAAGAAAAGATTTGGTCCATTGTTGGTCCATCTTGTTGAAGCAAAAGGTTGAGGACACTTTTTGCTGCGAACAATACATTGAGAACCATTATGAGTTGCTAGATTTTCTTGTTGCGAGCTATGATAACAAGGAAATTGCCTTGAATTGTGGAGCTATGTTGAGGGATTGCATCAGATTCCCTACTCTTGCAAAATACATATTGCAGTCTGCAAGCTTCGTTTTGTTCTTCAAATTTGTGGAGTTGCCTAACTTTGATGTAGCTTCTGATGCATTTTCTACCTTTAAGGATTTACTTACTAAACATGGAACGGTGGTATCTGAATTTTTGACCGCGCACTATGATGAGTTCTTCGATCTGTATGAAAAACTGTTGACATCTCCTAACTACGTGACTAGGAGGCAGTCTTTAAAGCTTCTTACGGATTTCCTATTGGAGCCTCCTAATTCTCATATAATGAAATGCTACATTTTAGAAGTTCGGTTCTTGAAAGTCATGATGATGCTGCTGAAGGATTCAAGCAAAAACATCCAAATCGCTGCCTTCCACATTTTCAAGGTCTTTGTTGCTAATCCTAATAAGCCCCGAGAAGTCAAGGTTATTCTGGCCAAGAACCATGAAAAATTGACAGAGTTGCTCCAGAATCTTTCTGCTGGAAAAGGTGATGAAGATGACCAATTTGAAGAGGAAAAGGAGCTAATCATCAAGGAAATTGAATTAGTAACCCAACAACCTAATCTTGGACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

346

Amino Acids

39.62

Weight (kDa)

5.27

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 5 - 217 6.1e-67 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 623
AciI CCGC 1 cut(s) 621
AcoI YGGCCR 1 cut(s) 894
AcsI RAATTY 3 cut(s) 145, 521, 611
AcuI CTGAAG 1 cut(s) 818
AfiI CCNNNNNNNGG 4 cut(s) 46, 121, 278, 1028
AgsI TTSAA 9 cut(s) 19, 328, 433, 520, 778, 807, 844, 971, 1004
AluBI AGCT 8 cut(s) 194, 217, 408, 443, 504, 551, 703, 985
AluI AGCT 8 cut(s) 194, 217, 408, 443, 504, 551, 703, 985
Alw26I GTCTC 1 cut(s) 179
Ama87I CYCGRG 1 cut(s) 873
AoxI GGCC 3 cut(s) 29, 57, 894
ApeKI GCWGC 3 cut(s) 354, 793, 827
ApoI RAATTY 3 cut(s) 145, 521, 611
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 3 cut(s) 30, 304, 315
AsuHPI GGTGA 1 cut(s) 962
AvaI CYCGRG 1 cut(s) 873
AvaII GGWCC 2 cut(s) 304, 315
BaeI ACNNNNGTAYC 2 cut(s) 588, 621
BalI TGGCCA 1 cut(s) 896
BbvI GCAGC 3 cut(s) 341, 780, 814
BccI CCATC 4 cut(s) 68, 173, 276, 326
BceAI ACGGC 1 cut(s) 7
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 4 cut(s) 257, 389, 684, 1036
BglI GCCNNNNNGGC 1 cut(s) 28
BisI GCNGC 3 cut(s) 355, 794, 828
BlsI GCNGC 3 cut(s) 356, 795, 829
Bme18I GGWCC 2 cut(s) 304, 315
BmeT110I CYCGRG 1 cut(s) 873
BmgT120I GGNCC 3 cut(s) 30, 304, 315
BmiI GGNNCC 1 cut(s) 726
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 3 cut(s) 468, 547, 780
BmuI ACTGGG 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 911
BsaAI YACGTR 1 cut(s) 679
BsaJI CCNNGG 1 cut(s) 54
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 121, 278, 1028
Bse1I ACTGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 3 cut(s) 247, 287, 816
BseLI CCNNNNNNNGG 4 cut(s) 46, 121, 278, 1028
BseNI ACTGG 1 cut(s) 282
BseXI GCAGC 3 cut(s) 341, 780, 814
Bsh1236I CGCG 1 cut(s) 623
BshFI GGCC 3 cut(s) 31, 59, 896
BsiHKCI CYCGRG 1 cut(s) 873
BslI CCNNNNNNNGG 4 cut(s) 46, 121, 278, 1028
BsmAI GTCTC 1 cut(s) 179
BsmBI CGTCTC 1 cut(s) 179
BsnI GGCC 3 cut(s) 31, 59, 896
BsoBI CYCGRG 1 cut(s) 873
Bsp143I GATC 1 cut(s) 643
BspACI CCGC 1 cut(s) 621
BspANI GGCC 3 cut(s) 31, 59, 896
BspFNI CGCG 1 cut(s) 623
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 1 cut(s) 726
BspQI GCTCTTC 1 cut(s) 258
BsrI ACTGG 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 1 cut(s) 643
BssT1I CCWWGG 1 cut(s) 54
Bst4CI ACNGT 3 cut(s) 97, 601, 660
Bst6I CTCTTC 3 cut(s) 126, 258, 966
BstBAI YACGTR 1 cut(s) 679
BstC8I GCNNGC 2 cut(s) 406, 502
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 3 cut(s) 247, 287, 816
BstFNI CGCG 1 cut(s) 623
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 1 cut(s) 646
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 1 cut(s) 643
BstMWI GCNNNNNNNGC 2 cut(s) 28, 557
BstUI CGCG 1 cut(s) 623
BstV1I GCAGC 3 cut(s) 341, 780, 814
BsuRI GGCC 3 cut(s) 31, 59, 896
BtsCI GGATG 3 cut(s) 247, 287, 816
Cac8I GCNNGC 2 cut(s) 406, 502
CciI TCATGA 1 cut(s) 783
CfoI GCGC 1 cut(s) 625
Cfr13I GGNCC 3 cut(s) 30, 304, 315
CviAII CATG 3 cut(s) 593, 784, 905
DpnI GATC 1 cut(s) 645
DpnII GATC 1 cut(s) 643
DraI TTTAAA 1 cut(s) 699
EaeI YGGCCR 1 cut(s) 894
Eam1104I CTCTTC 3 cut(s) 126, 258, 966
EarI CTCTTC 3 cut(s) 126, 258, 966
Eco130I CCWWGG 1 cut(s) 54
Eco47I GGWCC 2 cut(s) 304, 315
Eco57I CTGAAG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 873
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoT14I CCWWGG 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 3 cut(s) 596, 787, 908
FatI CATG 3 cut(s) 592, 783, 904
Fnu4HI GCNGC 3 cut(s) 355, 794, 828
FokI GGATG 3 cut(s) 254, 294, 803
Fsp4HI GCNGC 3 cut(s) 355, 794, 828
FspBI CTAG 4 cut(s) 257, 389, 684, 1036
GlaI GCGC 1 cut(s) 624
GluI GCNGC 3 cut(s) 355, 794, 828
GsuI CTGGAG 1 cut(s) 911
HaeIII GGCC 3 cut(s) 31, 59, 896
HhaI GCGC 1 cut(s) 625
Hin1II CATG 3 cut(s) 596, 787, 908
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HindIII AAGCTT 2 cut(s) 502, 701
HinfI GANTC 4 cut(s) 86, 465, 803, 931
HphI GGTGA 1 cut(s) 962
Hpy166II GTNNAC 2 cut(s) 262, 663
Hpy188I TCNGA 4 cut(s) 176, 464, 556, 610
Hpy188III TCNNGA 5 cut(s) 151, 226, 775, 784, 928
Hpy8I GTNNAC 2 cut(s) 262, 663
Hpy99I CGWCG 2 cut(s) 28, 101
HpyAV CCTTC 2 cut(s) 793, 841
HpyCH4III ACNGT 3 cut(s) 97, 601, 660
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 6 cut(s) 234, 459, 479, 493, 500, 560
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 557
HpySE526I ACGT 1 cut(s) 678
Hsp92II CATG 3 cut(s) 596, 787, 908
HspAI GCGC 1 cut(s) 623
Kzo9I GATC 1 cut(s) 643
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 4 cut(s) 440, 724, 930, 982
LpnPI CCDG 6 cut(s) 29, 104, 263, 878, 927, 941
Lsp1109I GCAGC 3 cut(s) 341, 780, 814
LweI GCATC 3 cut(s) 468, 547, 780
MaeI CTAG 4 cut(s) 257, 389, 684, 1036
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 3 cut(s) 267, 679, 1009
MalI GATC 1 cut(s) 645
MboI GATC 1 cut(s) 643
MboII GAAGA 7 cut(s) 7, 143, 245, 508, 631, 968, 983
MlsI TGGCCA 1 cut(s) 896
MluNI TGGCCA 1 cut(s) 896
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 2 cut(s) 108, 154
Mox20I TGGCCA 1 cut(s) 896
Mph1103I ATGCAT 1 cut(s) 562
MscI TGGCCA 1 cut(s) 896
MseI TTAA 2 cut(s) 573, 698
MslI CAYNNNNRTG 2 cut(s) 630, 788
Msp20I TGGCCA 1 cut(s) 896
MvnI CGCG 1 cut(s) 623
MwoI GCNNNNNNNGC 2 cut(s) 28, 557
NdeII GATC 1 cut(s) 643
NlaIII CATG 3 cut(s) 596, 787, 908
NlaIV GGNNCC 1 cut(s) 726
NmuCI GTSAC 1 cut(s) 679
NsiI ATGCAT 1 cut(s) 562
PagI TCATGA 1 cut(s) 783
PciSI GCTCTTC 1 cut(s) 258
PfeI GAWTC 3 cut(s) 465, 803, 931
PkrI GCNGC 3 cut(s) 356, 795, 829
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 679
PspN4I GGNNCC 1 cut(s) 726
PspPI GGNCC 3 cut(s) 30, 304, 315
RseI CAYNNNNRTG 2 cut(s) 630, 788
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 2 cut(s) 573, 698
SatI GCNGC 3 cut(s) 355, 794, 828
Sau3AI GATC 1 cut(s) 643
Sau96I GGNCC 3 cut(s) 30, 304, 315
SchI GAGTC 1 cut(s) 80
SfaNI GCATC 3 cut(s) 468, 547, 780
SinI GGWCC 2 cut(s) 304, 315
SmiMI CAYNNNNRTG 2 cut(s) 630, 788
SsiI CCGC 1 cut(s) 621
SspMI CTAG 4 cut(s) 257, 389, 684, 1036
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 3 cut(s) 97, 601, 660
TaiI ACGT 1 cut(s) 681
TaqI TCGA 5 cut(s) 26, 84, 117, 225, 642
TfiI GAWTC 3 cut(s) 465, 803, 931
Tru1I TTAA 2 cut(s) 573, 698
Tru9I TTAA 2 cut(s) 573, 698
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 3 cut(s) 354, 793, 827
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 4 cut(s) 666, 761, 921, 969
TspGWI ACGGA 3 cut(s) 203, 215, 725
VpaK11BI GGWCC 2 cut(s) 304, 315
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 3 cut(s) 145, 521, 611
XcmI CCANNNNNNNNNTGG 1 cut(s) 279
XspI CTAG 4 cut(s) 257, 389, 684, 1036
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.