Rroxscaffold_5G00362270

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009246
Physical Location & Seq
Reverse (-)
43165145 .. 43169118
3974 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00362270.1

Sequence Viewer

Length: 1038 bp
ATGTCGTTCTCGTTCTTCAAGCCATCGAGGCCCAAAACCCTCAGGAGGTCGCCAAGGCCATTCACGACACCCTCACTGCCCTCGACACCCAGACTGTCGCAGAAGTTAAAGCCCTCGAAAAGTTATGTTCTTGCTGTGAATTTGCTTCTTCCATTGTATATAATTGATGCGTTGGAAGAGGTTGAAAAGAATTTCGGGATTATGAGATGTATGCTTGTGGGAGATGCGGAGACGGAACCTAATATGGAACAAGTTTCGCAGCTTGTGCTCGAGATATGTAAGGAGGATGTTCTTGCTCTTCTAGTTCACAAGTTACCTATACTGGGATGGGAAGCAAGAAAAGATTTGGTCCATTGTTGGTCCGTGTTATTGAAGCAAAAGGTTGAGGATACTTATTGCTGCGAACAATACATTGAGAACCATACTGAGTTGCTAGACTTTCTGGTCGTGAGCTATGATAACAAGGAAATTGCCTTAAATTGTGGAGCTATGTTGAGGGATTGCATCAGATTCCCTATTCTTGCAAAATTCATATTGGAGTCTGCAAGCTTTGTTTTGTTCTTCAAATTTGTGGAGTTGCCTAACTTTGATGTAGCTTCTGATGCATTTTCTACCTTTAAGGACTTACTTACTAAACATGGAACAGTGGTATCTGAATTTTTGACTGCGCACTATGATGAGTTCTTTGATCTGTATGAAAAACTGTTGACATCTCCTAACTACGTGACTAGGAGGCAATCTTTAAAGCTTCTTACGGATTTCCTATTGGAGCCTCCAAATTCTCATATAATGAAACGCTACATTTTAGAAGTTCGGTTCTTGAAAGTCATGATGATGCTGCTGAAGGTCTTTGTTGCTAATCCTAATAAGCCTCGAGAAGTCAAGGTTATTCTGGCTAAGAACCATGCAAAATTGACGGAGTTGCTCCAGAATCTTTCTGCTGGAAAAGGTGATGAAGATGACCAATTCGAAGAGGAAAAGGAGCTAATCATCAAGGAAATTGAATTAGTAACCCAACAACCTAATCTTGGATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

345

Amino Acids

39.88

Weight (kDa)

5.9

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 57 - 335 1.2e-96 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 443
Acc16I TGCGCA 1 cut(s) 669
AciI CCGC 1 cut(s) 227
AcsI RAATTY 6 cut(s) 139, 190, 527, 566, 656, 778
AcuI CTGAAG 1 cut(s) 863
AfiI CCNNNNNNNGG 3 cut(s) 45, 323, 1028
AgsI TTSAA 6 cut(s) 19, 185, 373, 565, 823, 1004
AluBI AGCT 7 cut(s) 262, 453, 488, 549, 596, 748, 985
AluI AGCT 7 cut(s) 262, 453, 488, 549, 596, 748, 985
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 224
Ama87I CYCGRG 2 cut(s) 269, 873
AoxI GGCC 2 cut(s) 29, 56
ApeKI GCWGC 3 cut(s) 259, 399, 838
ApoI RAATTY 6 cut(s) 139, 190, 527, 566, 656, 778
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 3 cut(s) 30, 349, 360
AsuHPI GGTGA 1 cut(s) 962
AsuII TTCGAA 1 cut(s) 969
AvaI CYCGRG 2 cut(s) 269, 873
AvaII GGWCC 2 cut(s) 349, 360
AxyI CCTNAGG 1 cut(s) 41
BaeI ACNNNNGTAYC 2 cut(s) 633, 666
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 271, 386, 825
BccI CCATC 2 cut(s) 31, 321
BciVI GTATCC 2 cut(s) 382, 1025
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 4 cut(s) 302, 434, 729, 1036
BfuI GTATCC 2 cut(s) 382, 1025
BglI GCCNNNNNGGC 1 cut(s) 28
BisI GCNGC 3 cut(s) 260, 400, 839
BlsI GCNGC 3 cut(s) 261, 401, 840
Bme18I GGWCC 2 cut(s) 349, 360
BmeT110I CYCGRG 2 cut(s) 269, 873
BmgT120I GGNCC 3 cut(s) 30, 349, 360
BmiI GGNNCC 2 cut(s) 237, 771
BmrI ACTGGG 1 cut(s) 332
BmsI GCATC 5 cut(s) 157, 214, 513, 592, 825
BmuI ACTGGG 1 cut(s) 332
BpmI CTGGAG 1 cut(s) 911
Bpu14I TTCGAA 1 cut(s) 969
BsaAI YACGTR 1 cut(s) 724
BsaJI CCNNGG 1 cut(s) 53
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 323, 1028
Bse1I ACTGG 1 cut(s) 327
Bse21I CCTNAGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 53
BseGI GGATG 2 cut(s) 292, 332
BseLI CCNNNNNNNGG 3 cut(s) 45, 323, 1028
BseMII CTCAG 2 cut(s) 55, 417
BseNI ACTGG 1 cut(s) 327
BseXI GCAGC 3 cut(s) 271, 386, 825
BshFI GGCC 2 cut(s) 31, 58
BsiHKAI GWGCWC 1 cut(s) 270
BsiHKCI CYCGRG 2 cut(s) 269, 873
BslI CCNNNNNNNGG 3 cut(s) 45, 323, 1028
BsmAI GTCTC 1 cut(s) 224
BsmBI CGTCTC 1 cut(s) 224
BsnI GGCC 2 cut(s) 31, 58
BsoBI CYCGRG 2 cut(s) 269, 873
Bsp119I TTCGAA 1 cut(s) 969
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 1 cut(s) 688
BspACI CCGC 1 cut(s) 227
BspANI GGCC 2 cut(s) 31, 58
BspCNI CTCAG 2 cut(s) 54, 418
BspHI TCATGA 1 cut(s) 828
BspLI GGNNCC 2 cut(s) 237, 771
BspQI GCTCTTC 1 cut(s) 303
BspT104I TTCGAA 1 cut(s) 969
BsrI ACTGG 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 53
BssMI GATC 1 cut(s) 688
BssT1I CCWWGG 1 cut(s) 53
Bst4CI ACNGT 3 cut(s) 96, 646, 705
Bst6I CTCTTC 3 cut(s) 171, 303, 966
BstAPI GCANNNNNTGC 1 cut(s) 265
BstBAI YACGTR 1 cut(s) 724
BstBI TTCGAA 1 cut(s) 969
BstC8I GCNNGC 1 cut(s) 547
BstDEI CTNAG 3 cut(s) 41, 426, 897
BstF5I GGATG 2 cut(s) 292, 332
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 1 cut(s) 691
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 1 cut(s) 688
BstMWI GCNNNNNNNGC 3 cut(s) 28, 265, 602
BstV1I GCAGC 3 cut(s) 271, 386, 825
Bsu36I CCTNAGG 1 cut(s) 41
BsuI GTATCC 2 cut(s) 382, 1025
BsuRI GGCC 2 cut(s) 31, 58
BtsCI GGATG 2 cut(s) 292, 332
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 2 cut(s) 74, 651
Cac8I GCNNGC 1 cut(s) 547
CciI TCATGA 1 cut(s) 828
CfoI GCGC 1 cut(s) 670
Cfr13I GGNCC 3 cut(s) 30, 349, 360
CviAII CATG 3 cut(s) 638, 829, 905
DdeI CTNAG 3 cut(s) 41, 426, 897
DpnI GATC 1 cut(s) 690
DpnII GATC 1 cut(s) 688
DraI TTTAAA 1 cut(s) 744
DrdI GACNNNNNNGTC 1 cut(s) 443
DseDI GACNNNNNNGTC 1 cut(s) 443
Eam1104I CTCTTC 3 cut(s) 171, 303, 966
EarI CTCTTC 3 cut(s) 171, 303, 966
Eco130I CCWWGG 1 cut(s) 53
Eco47I GGWCC 2 cut(s) 349, 360
Eco57I CTGAAG 1 cut(s) 863
Eco81I CCTNAGG 1 cut(s) 41
Eco88I CYCGRG 2 cut(s) 269, 873
EcoT14I CCWWGG 1 cut(s) 53
EcoT22I ATGCAT 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 53
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 3 cut(s) 641, 832, 908
FatI CATG 3 cut(s) 637, 828, 904
Fnu4HI GCNGC 3 cut(s) 260, 400, 839
FokI GGATG 2 cut(s) 299, 339
Fsp4HI GCNGC 3 cut(s) 260, 400, 839
FspBI CTAG 4 cut(s) 302, 434, 729, 1036
FspI TGCGCA 1 cut(s) 669
GlaI GCGC 1 cut(s) 669
GluI GCNGC 3 cut(s) 260, 400, 839
GsuI CTGGAG 1 cut(s) 911
HaeIII GGCC 2 cut(s) 31, 58
HhaI GCGC 1 cut(s) 670
Hin1II CATG 3 cut(s) 641, 832, 908
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HincII GTYRAC 1 cut(s) 708
HindII GTYRAC 1 cut(s) 708
HindIII AAGCTT 2 cut(s) 547, 746
HinfI GANTC 3 cut(s) 510, 539, 931
HphI GGTGA 1 cut(s) 962
Hpy166II GTNNAC 2 cut(s) 307, 708
Hpy188I TCNGA 3 cut(s) 509, 601, 655
Hpy188III TCNNGA 9 cut(s) 43, 64, 196, 271, 448, 820, 829, 875, 928
Hpy8I GTNNAC 2 cut(s) 307, 708
HpyAV CCTTC 1 cut(s) 838
HpyCH4III ACNGT 3 cut(s) 96, 646, 705
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 5 cut(s) 504, 524, 545, 605, 908
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 265, 602
HpyF3I CTNAG 3 cut(s) 41, 426, 897
HpySE526I ACGT 1 cut(s) 723
Hsp92II CATG 3 cut(s) 641, 832, 908
HspAI GCGC 1 cut(s) 668
Kzo9I GATC 1 cut(s) 688
LguI GCTCTTC 1 cut(s) 303
LmnI GCTCC 4 cut(s) 485, 769, 930, 982
LpnPI CCDG 7 cut(s) 28, 103, 308, 428, 878, 927, 941
Lsp1109I GCAGC 3 cut(s) 271, 386, 825
LweI GCATC 5 cut(s) 157, 214, 513, 592, 825
MaeI CTAG 4 cut(s) 302, 434, 729, 1036
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 3 cut(s) 312, 724, 1009
MalI GATC 1 cut(s) 690
MboI GATC 1 cut(s) 688
MboII GAAGA 7 cut(s) 7, 140, 188, 290, 553, 968, 983
MhlI GDGCHC 1 cut(s) 270
MlyI GAGTC 1 cut(s) 548
MmeI TCCRAC 1 cut(s) 153
Mph1103I ATGCAT 1 cut(s) 607
MseI TTAA 4 cut(s) 107, 476, 618, 743
MslI CAYNNNNRTG 2 cut(s) 675, 833
MwoI GCNNNNNNNGC 3 cut(s) 28, 265, 602
NdeII GATC 1 cut(s) 688
NlaIII CATG 3 cut(s) 641, 832, 908
NlaIV GGNNCC 2 cut(s) 237, 771
NmuCI GTSAC 1 cut(s) 724
NsbI TGCGCA 1 cut(s) 669
NsiI ATGCAT 1 cut(s) 607
NspV TTCGAA 1 cut(s) 969
PaeR7I CTCGAG 2 cut(s) 269, 873
PagI TCATGA 1 cut(s) 828
PciSI GCTCTTC 1 cut(s) 303
PfeI GAWTC 2 cut(s) 510, 931
PkrI GCNGC 3 cut(s) 261, 401, 840
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
Ppu21I YACGTR 1 cut(s) 724
PspN4I GGNNCC 2 cut(s) 237, 771
PspPI GGNCC 3 cut(s) 30, 349, 360
RseI CAYNNNNRTG 2 cut(s) 675, 833
SapI GCTCTTC 1 cut(s) 303
SaqAI TTAA 4 cut(s) 107, 476, 618, 743
SatI GCNGC 3 cut(s) 260, 400, 839
Sau3AI GATC 1 cut(s) 688
Sau96I GGNCC 3 cut(s) 30, 349, 360
SchI GAGTC 1 cut(s) 548
SduI GDGCHC 1 cut(s) 270
SfaNI GCATC 5 cut(s) 157, 214, 513, 592, 825
Sfr274I CTCGAG 2 cut(s) 269, 873
SfuI TTCGAA 1 cut(s) 969
SinI GGWCC 2 cut(s) 349, 360
SlaI CTCGAG 2 cut(s) 269, 873
SmiMI CAYNNNNRTG 2 cut(s) 675, 833
SmlI CTYRAG 2 cut(s) 269, 873
SmoI CTYRAG 2 cut(s) 269, 873
SsiI CCGC 1 cut(s) 227
SspMI CTAG 4 cut(s) 302, 434, 729, 1036
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 3 cut(s) 96, 646, 705
TaiI ACGT 1 cut(s) 726
TaqI TCGA 6 cut(s) 26, 83, 116, 270, 874, 969
TfiI GAWTC 2 cut(s) 510, 931
Tru1I TTAA 4 cut(s) 107, 476, 618, 743
Tru9I TTAA 4 cut(s) 107, 476, 618, 743
TscAI CASTG 2 cut(s) 81, 651
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 3 cut(s) 259, 399, 838
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 4 cut(s) 520, 711, 806, 969
TspGWI ACGGA 4 cut(s) 248, 352, 770, 932
TspRI CASTG 2 cut(s) 81, 651
VpaK11BI GGWCC 2 cut(s) 349, 360
XapI RAATTY 6 cut(s) 139, 190, 527, 566, 656, 778
XhoI CTCGAG 2 cut(s) 269, 873
XspI CTAG 4 cut(s) 302, 434, 729, 1036
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.