Rh4DG218800

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009246
Physical Location & Seq
Reverse (-)
41917805 .. 41921836
4032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG218800.1

Sequence Viewer

Length: 1194 bp
ATGCTGTTAACGGATCCGCACTGGCACGAAAAAGACCGGGGCTCTTTTCCCAATCGTCCTTCTTCACCAAACTCACAGCTAGCAGCTGTAAATTTCTTCCACAGAGAAAGAGACAGAGCCAGAGAGAAAGAGAGGGAATTTGATTCCCCATCGATCATGTCGTTCTCGTTCTTCAAGCCTTCGAGGCCCAAAACCCCTCAGGAGGTCGCCAAGGCCATTCACGACAGCCTCACTGCCCTCGACACCCAGACCGTCGCCGAAGTTAAAGCCCTTGAAAAGGCGTTGGAAGAGGTTGAAAAGAATTTCGGGATTATGAGATGTATGCTTGTTGGAGATGCGGAGACAGAACCTAATATGGAACAAGTTTCGCAGCTTGTGCTCGAGATATGTAAGGAGGATGTTCTTGCTCTTCTAGTTCACAAGTTACCTATACTGGGATGGGAAGCAAGAAAAGATTTGGTCCATTGTTGGTCCGTGTTATTGAAGCAAAAGGTTGAGGATACTTATTGCTGCGAACAATACATTGAGGACCATACTGAGTTGCTAGACTTTCTGGTCGTGAGCTATGATAACAAGGAAATTGCCTTAAATTGTGGAGCTATGTTGAGGGATTGCATCAGATTCCCTATTCTTGCAAAATACATATTGGAGTCTGCAAGCTTTGTTTTGTTCTTCAAATTTGTGGAGTTGCCTAACTTTGATGTAGCTTCTGATGCATTTTCTACCTTTAAGGACTTACTTACTAAACATGGAACGGTGGTATCTGAATTTTTGACTGCGCACTATGATGAGTTCTTCGATCTGTATGAAAAACTGTTGACATCTCCTAACTACGTGACTAGGAGGCAATCTTTAAAGCTTCTTACGGATTTCCTATTGGAACCTCCAAATTCTCATATAATGAAACGCTACATTTTAGAAGTTCGGTTCTTGAAAGTCATGATGATGCTGCTGAAGGATTCAAGCAAAAACATCCAGATCGCTGCCTTTCACATTTTCAAGGTCTTTGTTGCTAATCCTAATAAGCCTCGAGAAGTCAAGGTTATTCTGGCTAAGAACCATGCAAAATTGACGGAGTTGCTCCAGAATCTTTCTGCTGGAAAAGGTGATGAAGATGACCAATTTGAAGAGGAAAAGGAGCTAATCATCAAGGAAATTGAATTAGTAACCCAACAACCTAATCTTGGATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

397

Amino Acids

45.9

Weight (kDa)

5.49

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 57 - 387 2.5e-123 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
Acc16I TGCGCA 1 cut(s) 780
AciI CCGC 2 cut(s) 17, 338
AclWI GGATC 2 cut(s) 8, 21
AcsI RAATTY 6 cut(s) 91, 137, 301, 677, 767, 889
AcuI CTGAAG 1 cut(s) 974
AfiI CCNNNNNNNGG 4 cut(s) 202, 277, 434, 1184
AluBI AGCT 9 cut(s) 79, 86, 373, 564, 599, 660, 707, 859, 1141
AluI AGCT 9 cut(s) 79, 86, 373, 564, 599, 660, 707, 859, 1141
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 2 cut(s) 105, 335
AlwI GGATC 2 cut(s) 8, 21
Ama87I CYCGRG 2 cut(s) 380, 1029
AoxI GGCC 2 cut(s) 185, 213
ApeKI GCWGC 5 cut(s) 83, 370, 510, 949, 983
ApoI RAATTY 6 cut(s) 91, 137, 301, 677, 767, 889
AspLEI GCGC 1 cut(s) 781
AspS9I GGNCC 4 cut(s) 186, 460, 471, 529
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 57, 1118
AsuNHI GCTAGC 1 cut(s) 79
AvaI CYCGRG 2 cut(s) 380, 1029
AvaII GGWCC 3 cut(s) 460, 471, 529
AxyI CCTNAGG 1 cut(s) 198
BaeI ACNNNNGTAYC 2 cut(s) 744, 777
BamHI GGATCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 5 cut(s) 95, 382, 497, 936, 970
BccI CCATC 2 cut(s) 157, 432
BciVI GTATCC 2 cut(s) 493, 1181
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 2 cut(s) 105, 335
BfaI CTAG 5 cut(s) 80, 413, 545, 840, 1192
BfuI GTATCC 2 cut(s) 493, 1181
BglI GCCNNNNNGGC 1 cut(s) 184
BisI GCNGC 5 cut(s) 84, 371, 511, 950, 984
BlsI GCNGC 5 cut(s) 85, 372, 512, 951, 985
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 3 cut(s) 460, 471, 529
BmeT110I CYCGRG 2 cut(s) 380, 1029
BmgT120I GGNCC 4 cut(s) 186, 460, 471, 529
BmiI GGNNCC 2 cut(s) 15, 882
BmrFI CCNGG 1 cut(s) 38
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 4 cut(s) 325, 624, 703, 936
BmtI GCTAGC 1 cut(s) 83
BmuI ACTGGG 1 cut(s) 443
BpmI CTGGAG 1 cut(s) 1067
BpuMI CCSGG 1 cut(s) 38
Bsa29I ATCGAT 1 cut(s) 152
BsaAI YACGTR 1 cut(s) 835
BsaJI CCNNGG 2 cut(s) 37, 210
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 277, 434, 1184
Bse1I ACTGG 2 cut(s) 26, 438
Bse21I CCTNAGG 1 cut(s) 198
BseCI ATCGAT 1 cut(s) 152
BseDI CCNNGG 2 cut(s) 37, 210
BseGI GGATG 3 cut(s) 403, 443, 972
BseLI CCNNNNNNNGG 4 cut(s) 202, 277, 434, 1184
BseMII CTCAG 2 cut(s) 212, 528
BseNI ACTGG 2 cut(s) 26, 438
BseXI GCAGC 5 cut(s) 95, 382, 497, 936, 970
BshFI GGCC 2 cut(s) 187, 215
BshVI ATCGAT 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 2 cut(s) 380, 1029
BsiSI CCGG 1 cut(s) 37
BslI CCNNNNNNNGG 4 cut(s) 202, 277, 434, 1184
BsmAI GTCTC 2 cut(s) 105, 335
BsnI GGCC 2 cut(s) 187, 215
BsoBI CYCGRG 2 cut(s) 380, 1029
Bsp1286I GDGCHC 2 cut(s) 44, 381
Bsp143I GATC 4 cut(s) 13, 153, 799, 978
BspACI CCGC 2 cut(s) 17, 338
BspANI GGCC 2 cut(s) 187, 215
BspCNI CTCAG 2 cut(s) 211, 529
BspDI ATCGAT 1 cut(s) 152
BspHI TCATGA 1 cut(s) 939
BspLI GGNNCC 2 cut(s) 15, 882
BspOI GCTAGC 1 cut(s) 83
BspPI GGATC 2 cut(s) 8, 21
BspQI GCTCTTC 1 cut(s) 414
BsrI ACTGG 2 cut(s) 26, 438
BssECI CCNNGG 2 cut(s) 37, 210
BssMI GATC 4 cut(s) 13, 153, 799, 978
BssT1I CCWWGG 1 cut(s) 210
Bst4CI ACNGT 3 cut(s) 253, 757, 816
Bst6I CTCTTC 3 cut(s) 282, 414, 1122
BstAPI GCANNNNNTGC 1 cut(s) 376
BstBAI YACGTR 1 cut(s) 835
BstC8I GCNNGC 2 cut(s) 81, 658
BstDEI CTNAG 3 cut(s) 198, 537, 1053
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 3 cut(s) 403, 443, 972
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 4 cut(s) 16, 156, 802, 981
BstMAI GTCTC 2 cut(s) 105, 335
BstMBI GATC 4 cut(s) 13, 153, 799, 978
BstMWI GCNNNNNNNGC 3 cut(s) 184, 376, 713
BstSCI CCNGG 1 cut(s) 36
BstV1I GCAGC 5 cut(s) 95, 382, 497, 936, 970
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
Bsu15I ATCGAT 1 cut(s) 152
Bsu36I CCTNAGG 1 cut(s) 198
BsuI GTATCC 2 cut(s) 493, 1181
BsuRI GGCC 2 cut(s) 187, 215
BsuTUI ATCGAT 1 cut(s) 152
BtsCI GGATG 3 cut(s) 403, 443, 972
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 2 cut(s) 19, 231
Cac8I GCNNGC 2 cut(s) 81, 658
CciI TCATGA 1 cut(s) 939
CfoI GCGC 1 cut(s) 781
Cfr13I GGNCC 4 cut(s) 186, 460, 471, 529
ClaI ATCGAT 1 cut(s) 152
CviAII CATG 4 cut(s) 157, 749, 940, 1061
DdeI CTNAG 3 cut(s) 198, 537, 1053
DpnI GATC 4 cut(s) 15, 155, 801, 980
DpnII GATC 4 cut(s) 13, 153, 799, 978
DraI TTTAAA 1 cut(s) 855
DrdI GACNNNNNNGTC 1 cut(s) 554
DseDI GACNNNNNNGTC 1 cut(s) 554
Eam1104I CTCTTC 3 cut(s) 282, 414, 1122
EarI CTCTTC 3 cut(s) 282, 414, 1122
Eco130I CCWWGG 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 3 cut(s) 460, 471, 529
Eco57I CTGAAG 1 cut(s) 974
Eco81I CCTNAGG 1 cut(s) 198
Eco88I CYCGRG 2 cut(s) 380, 1029
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoT14I CCWWGG 1 cut(s) 210
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 4 cut(s) 160, 752, 943, 1064
FatI CATG 4 cut(s) 156, 748, 939, 1060
Fnu4HI GCNGC 5 cut(s) 84, 371, 511, 950, 984
FokI GGATG 3 cut(s) 410, 450, 959
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 5 cut(s) 84, 371, 511, 950, 984
FspBI CTAG 5 cut(s) 80, 413, 545, 840, 1192
FspI TGCGCA 1 cut(s) 780
GlaI GCGC 1 cut(s) 780
GluI GCNGC 5 cut(s) 84, 371, 511, 950, 984
GsuI CTGGAG 1 cut(s) 1067
HaeIII GGCC 2 cut(s) 187, 215
HapII CCGG 1 cut(s) 37
HhaI GCGC 1 cut(s) 781
Hin1II CATG 4 cut(s) 160, 752, 943, 1064
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HincII GTYRAC 2 cut(s) 9, 819
HindII GTYRAC 2 cut(s) 9, 819
HindIII AAGCTT 2 cut(s) 658, 857
HinfI GANTC 5 cut(s) 143, 621, 650, 959, 1087
HpaI GTTAAC 1 cut(s) 9
HpaII CCGG 1 cut(s) 37
HphI GGTGA 2 cut(s) 57, 1118
Hpy166II GTNNAC 3 cut(s) 9, 418, 819
Hpy188I TCNGA 3 cut(s) 620, 712, 766
Hpy8I GTNNAC 3 cut(s) 9, 418, 819
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 3 cut(s) 69, 189, 949
HpyCH4III ACNGT 3 cut(s) 253, 757, 816
HpyCH4IV ACGT 1 cut(s) 834
HpyCH4V TGCA 5 cut(s) 615, 635, 656, 716, 1064
HpyF10VI GCNNNNNNNGC 3 cut(s) 184, 376, 713
HpyF3I CTNAG 3 cut(s) 198, 537, 1053
HpySE526I ACGT 1 cut(s) 834
Hsp92II CATG 4 cut(s) 160, 752, 943, 1064
HspAI GCGC 1 cut(s) 779
KspAI GTTAAC 1 cut(s) 9
Kzo9I GATC 4 cut(s) 13, 153, 799, 978
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 3 cut(s) 596, 1086, 1138
Lsp1109I GCAGC 5 cut(s) 95, 382, 497, 936, 970
LweI GCATC 4 cut(s) 325, 624, 703, 936
MaeI CTAG 5 cut(s) 80, 413, 545, 840, 1192
MaeII ACGT 1 cut(s) 834
MaeIII GTNAC 3 cut(s) 423, 835, 1165
MalI GATC 4 cut(s) 15, 155, 801, 980
MboI GATC 4 cut(s) 13, 153, 799, 978
MboII GAAGA 9 cut(s) 54, 88, 163, 299, 401, 664, 787, 1124, 1139
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 2 cut(s) 44, 381
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 2 cut(s) 264, 310
Mph1103I ATGCAT 1 cut(s) 718
MseI TTAA 5 cut(s) 8, 264, 587, 729, 854
MslI CAYNNNNRTG 2 cut(s) 786, 944
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 184, 376, 713
NciI CCSGG 1 cut(s) 38
NdeII GATC 4 cut(s) 13, 153, 799, 978
NheI GCTAGC 1 cut(s) 79
NlaIII CATG 4 cut(s) 160, 752, 943, 1064
NlaIV GGNNCC 2 cut(s) 15, 882
NmuCI GTSAC 1 cut(s) 835
NsbI TGCGCA 1 cut(s) 780
NsiI ATGCAT 1 cut(s) 718
PaeR7I CTCGAG 2 cut(s) 380, 1029
PagI TCATGA 1 cut(s) 939
PciSI GCTCTTC 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PfeI GAWTC 4 cut(s) 143, 621, 959, 1087
PkrI GCNGC 5 cut(s) 85, 372, 512, 951, 985
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
Ppu21I YACGTR 1 cut(s) 835
PspN4I GGNNCC 2 cut(s) 15, 882
PspPI GGNCC 4 cut(s) 186, 460, 471, 529
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 86
RseI CAYNNNNRTG 2 cut(s) 786, 944
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 5 cut(s) 8, 264, 587, 729, 854
SatI GCNGC 5 cut(s) 84, 371, 511, 950, 984
Sau3AI GATC 4 cut(s) 13, 153, 799, 978
Sau96I GGNCC 4 cut(s) 186, 460, 471, 529
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 2 cut(s) 44, 381
SfaNI GCATC 4 cut(s) 325, 624, 703, 936
Sfr274I CTCGAG 2 cut(s) 380, 1029
SinI GGWCC 3 cut(s) 460, 471, 529
SlaI CTCGAG 2 cut(s) 380, 1029
SmiMI CAYNNNNRTG 2 cut(s) 786, 944
SmlI CTYRAG 2 cut(s) 380, 1029
SmoI CTYRAG 2 cut(s) 380, 1029
SsiI CCGC 2 cut(s) 17, 338
SspMI CTAG 5 cut(s) 80, 413, 545, 840, 1192
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 3 cut(s) 253, 757, 816
TaiI ACGT 1 cut(s) 837
TaqI TCGA 6 cut(s) 152, 182, 240, 381, 798, 1030
TfiI GAWTC 4 cut(s) 143, 621, 959, 1087
Tru1I TTAA 5 cut(s) 8, 264, 587, 729, 854
Tru9I TTAA 5 cut(s) 8, 264, 587, 729, 854
TscAI CASTG 2 cut(s) 26, 238
TseFI GTSAC 1 cut(s) 835
TseI GCWGC 5 cut(s) 83, 370, 510, 949, 983
Tsp45I GTSAC 1 cut(s) 835
TspDTI ATGAA 3 cut(s) 822, 917, 1125
TspGWI ACGGA 4 cut(s) 26, 463, 881, 1088
TspRI CASTG 2 cut(s) 26, 238
VpaK11BI GGWCC 3 cut(s) 460, 471, 529
XagI CCTNNNNNAGG 1 cut(s) 275
XapI RAATTY 6 cut(s) 91, 137, 301, 677, 767, 889
XhoI CTCGAG 2 cut(s) 380, 1029
XspI CTAG 5 cut(s) 80, 413, 545, 840, 1192
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.