RchiOBHm_Chr4g0419281

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009246
Physical Location & Seq
Reverse (-)
44731595 .. 44736162
4568 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38919

Sequence Viewer

Length: 1038 bp
ATGTCGTTCTCGTTCTTCAAGCCGTCGAGGCCCAAAACCCCTCAGGAGGTCTCCAAGGCCATTCACGACAGCCTCACTGCCCTCGACACCCAGACCGTCGCCGAAGTTAAAGCCCTCGAAAAGGCGTTGGAAGAGGTTGAAAAGAATTTCGGGATTATGAGATGTATGCTTGTCGGGGATGCGGAGACGGAACCTAATATGGAACAAGTTTCGCAGCTTGTGCTCGAGATATGTAAGGAGGATGTTCTTGCTCTTCTAGTTCACAAGTTACCTATACTGGGATGGGAAGCAAGAAAAGATTTGGTCCATTGTTGGTCCGTGTTATTGAAGCAAAAGGTTGAGGATACTTATTGCTGCGAACAATACATTGAGGACCATACTGAGTTGCTAGACTTTCTGGTCGTGAGCTATGATAACAAGGAAATTGCCTTAAATTGTGGAGCTATGTTGAGGGATTGCATCAGATTCCCTATTCTTGCAAAATACATATTGGAGTCTGCAAGCTTTGTTTTGTTCTTCAAATTTGTGGAGTTGCCTAACTTTGATGTAGCTTCTGATGCATTTTCTACCTTTAAGGACTTACTTACTAAACATGGAATGGTGGTATCTGAATTTTTGACTGCACACTATGATGAGTTCTTCGATCTGTATGAAAAACTGTTAACATCTCCTAACTACGTGACTAGGAGGCAATCTTTAAAGCTTCTTACGGATTTCCTATTGGAACCTCCAAATTCTCATATAATGAAACGCTACATTTTAGAAGTTCGGTTCTTGAAAGTCATGATGATGCTGCTGAAGGATTCAAGCAAAAACATCCAGATTGCTGCCTTCCACATTTTCAAGGTCTTTGTTGCTAATCCTAATAAGCCTCGAGAAGTCAAGGTTATTCTGGCTAAGAACCATGCAAAATTGACGGAGTTGCTCCAGAATCTTTCTGCTGGAAAAGGTGATGAAGATGACCAATTTGAAGAGGAAAAGGAGCTAATCATCAAGGAAATTGAATTAGTAACCCAACAACCTAATCTTGGATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

345

Amino Acids

39.77

Weight (kDa)

5.27

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 5 - 335 5.4e-123 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
AciI CCGC 1 cut(s) 182
AcsI RAATTY 4 cut(s) 145, 521, 611, 733
AcuI CTGAAG 1 cut(s) 818
AfiI CCNNNNNNNGG 4 cut(s) 46, 121, 278, 1028
AgsI TTSAA 9 cut(s) 19, 140, 328, 520, 778, 807, 844, 971, 1004
AluBI AGCT 7 cut(s) 217, 408, 443, 504, 551, 703, 985
AluI AGCT 7 cut(s) 217, 408, 443, 504, 551, 703, 985
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 2 cut(s) 55, 179
Ama87I CYCGRG 2 cut(s) 224, 873
AoxI GGCC 2 cut(s) 29, 57
ApeKI GCWGC 4 cut(s) 214, 354, 793, 827
ApoI RAATTY 4 cut(s) 145, 521, 611, 733
AspS9I GGNCC 4 cut(s) 30, 304, 315, 373
AsuHPI GGTGA 1 cut(s) 962
AvaI CYCGRG 2 cut(s) 224, 873
AvaII GGWCC 3 cut(s) 304, 315, 373
AxyI CCTNAGG 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 4 cut(s) 226, 341, 780, 814
BccI CCATC 1 cut(s) 276
BceAI ACGGC 1 cut(s) 7
BciVI GTATCC 2 cut(s) 337, 1025
BcoDI GTCTC 2 cut(s) 55, 179
BfaI CTAG 4 cut(s) 257, 389, 684, 1036
BfuI GTATCC 2 cut(s) 337, 1025
BglI GCCNNNNNGGC 1 cut(s) 28
BisI GCNGC 4 cut(s) 215, 355, 794, 828
BlsI GCNGC 4 cut(s) 216, 356, 795, 829
Bme18I GGWCC 3 cut(s) 304, 315, 373
BmeT110I CYCGRG 2 cut(s) 224, 873
BmgT120I GGNCC 4 cut(s) 30, 304, 315, 373
BmiI GGNNCC 2 cut(s) 192, 726
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 4 cut(s) 169, 468, 547, 780
BmuI ACTGGG 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 911
BsaAI YACGTR 1 cut(s) 679
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 54
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 121, 278, 1028
Bse1I ACTGG 1 cut(s) 282
Bse21I CCTNAGG 1 cut(s) 42
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 4 cut(s) 184, 247, 287, 816
BseLI CCNNNNNNNGG 4 cut(s) 46, 121, 278, 1028
BseMII CTCAG 2 cut(s) 56, 372
BseNI ACTGG 1 cut(s) 282
BseXI GCAGC 4 cut(s) 226, 341, 780, 814
BsgI GTGCAG 1 cut(s) 606
BshFI GGCC 2 cut(s) 31, 59
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 2 cut(s) 224, 873
BslI CCNNNNNNNGG 4 cut(s) 46, 121, 278, 1028
BsmAI GTCTC 2 cut(s) 55, 179
BsmBI CGTCTC 1 cut(s) 179
BsnI GGCC 2 cut(s) 31, 59
Bso31I GGTCTC 1 cut(s) 55
BsoBI CYCGRG 2 cut(s) 224, 873
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 1 cut(s) 643
BspACI CCGC 1 cut(s) 182
BspANI GGCC 2 cut(s) 31, 59
BspCNI CTCAG 2 cut(s) 55, 373
BspHI TCATGA 1 cut(s) 783
BspLI GGNNCC 2 cut(s) 192, 726
BspQI GCTCTTC 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 55
BsrI ACTGG 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 1 cut(s) 643
BssT1I CCWWGG 1 cut(s) 54
Bst4CI ACNGT 2 cut(s) 97, 660
Bst6I CTCTTC 3 cut(s) 126, 258, 966
BstAPI GCANNNNNTGC 1 cut(s) 220
BstBAI YACGTR 1 cut(s) 679
BstC8I GCNNGC 1 cut(s) 502
BstDEI CTNAG 3 cut(s) 42, 381, 897
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 4 cut(s) 184, 247, 287, 816
BstKTI GATC 1 cut(s) 646
BstMAI GTCTC 2 cut(s) 55, 179
BstMBI GATC 1 cut(s) 643
BstMWI GCNNNNNNNGC 3 cut(s) 28, 220, 557
BstV1I GCAGC 4 cut(s) 226, 341, 780, 814
Bsu36I CCTNAGG 1 cut(s) 42
BsuI GTATCC 2 cut(s) 337, 1025
BsuRI GGCC 2 cut(s) 31, 59
BtsCI GGATG 4 cut(s) 184, 247, 287, 816
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 502
CciI TCATGA 1 cut(s) 783
Cfr13I GGNCC 4 cut(s) 30, 304, 315, 373
CviAII CATG 3 cut(s) 593, 784, 905
DdeI CTNAG 3 cut(s) 42, 381, 897
DpnI GATC 1 cut(s) 645
DpnII GATC 1 cut(s) 643
DraI TTTAAA 1 cut(s) 699
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
Eam1104I CTCTTC 3 cut(s) 126, 258, 966
EarI CTCTTC 3 cut(s) 126, 258, 966
Eco130I CCWWGG 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 55
Eco47I GGWCC 3 cut(s) 304, 315, 373
Eco57I CTGAAG 1 cut(s) 818
Eco81I CCTNAGG 1 cut(s) 42
Eco88I CYCGRG 2 cut(s) 224, 873
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoT14I CCWWGG 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 3 cut(s) 596, 787, 908
FatI CATG 3 cut(s) 592, 783, 904
Fnu4HI GCNGC 4 cut(s) 215, 355, 794, 828
FokI GGATG 4 cut(s) 191, 254, 294, 803
Fsp4HI GCNGC 4 cut(s) 215, 355, 794, 828
FspBI CTAG 4 cut(s) 257, 389, 684, 1036
GluI GCNGC 4 cut(s) 215, 355, 794, 828
GsuI CTGGAG 1 cut(s) 911
HaeIII GGCC 2 cut(s) 31, 59
Hin1II CATG 3 cut(s) 596, 787, 908
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HindIII AAGCTT 2 cut(s) 502, 701
HinfI GANTC 4 cut(s) 465, 494, 803, 931
HpaI GTTAAC 1 cut(s) 663
HphI GGTGA 1 cut(s) 962
Hpy166II GTNNAC 2 cut(s) 262, 663
Hpy188I TCNGA 3 cut(s) 464, 556, 610
Hpy8I GTNNAC 2 cut(s) 262, 663
Hpy99I CGWCG 2 cut(s) 28, 101
HpyAV CCTTC 2 cut(s) 793, 841
HpyCH4III ACNGT 2 cut(s) 97, 660
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 6 cut(s) 459, 479, 500, 560, 623, 908
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 220, 557
HpyF3I CTNAG 3 cut(s) 42, 381, 897
HpySE526I ACGT 1 cut(s) 678
Hsp92II CATG 3 cut(s) 596, 787, 908
KspAI GTTAAC 1 cut(s) 663
Kzo9I GATC 1 cut(s) 643
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 3 cut(s) 440, 930, 982
LpnPI CCDG 8 cut(s) 29, 104, 263, 383, 833, 878, 927, 941
Lsp1109I GCAGC 4 cut(s) 226, 341, 780, 814
LweI GCATC 4 cut(s) 169, 468, 547, 780
MaeI CTAG 4 cut(s) 257, 389, 684, 1036
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 3 cut(s) 267, 679, 1009
MalI GATC 1 cut(s) 645
MboI GATC 1 cut(s) 643
MboII GAAGA 7 cut(s) 7, 143, 245, 508, 631, 968, 983
MhlI GDGCHC 1 cut(s) 225
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 1 cut(s) 108
Mph1103I ATGCAT 1 cut(s) 562
MseI TTAA 5 cut(s) 108, 431, 573, 662, 698
MslI CAYNNNNRTG 2 cut(s) 630, 788
MwoI GCNNNNNNNGC 3 cut(s) 28, 220, 557
NdeII GATC 1 cut(s) 643
NlaIII CATG 3 cut(s) 596, 787, 908
NlaIV GGNNCC 2 cut(s) 192, 726
NmuCI GTSAC 1 cut(s) 679
NsiI ATGCAT 1 cut(s) 562
PaeR7I CTCGAG 2 cut(s) 224, 873
PagI TCATGA 1 cut(s) 783
PciSI GCTCTTC 1 cut(s) 258
PfeI GAWTC 3 cut(s) 465, 803, 931
PkrI GCNGC 4 cut(s) 216, 356, 795, 829
PleI GAGTC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 502
Ppu21I YACGTR 1 cut(s) 679
PspN4I GGNNCC 2 cut(s) 192, 726
PspPI GGNCC 4 cut(s) 30, 304, 315, 373
RseI CAYNNNNRTG 2 cut(s) 630, 788
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 5 cut(s) 108, 431, 573, 662, 698
SatI GCNGC 4 cut(s) 215, 355, 794, 828
Sau3AI GATC 1 cut(s) 643
Sau96I GGNCC 4 cut(s) 30, 304, 315, 373
SchI GAGTC 1 cut(s) 503
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 4 cut(s) 169, 468, 547, 780
Sfr274I CTCGAG 2 cut(s) 224, 873
SinI GGWCC 3 cut(s) 304, 315, 373
SlaI CTCGAG 2 cut(s) 224, 873
SmiMI CAYNNNNRTG 2 cut(s) 630, 788
SmlI CTYRAG 2 cut(s) 224, 873
SmoI CTYRAG 2 cut(s) 224, 873
SsiI CCGC 1 cut(s) 182
SspMI CTAG 4 cut(s) 257, 389, 684, 1036
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 2 cut(s) 97, 660
TaiI ACGT 1 cut(s) 681
TaqI TCGA 6 cut(s) 26, 84, 117, 225, 642, 874
TfiI GAWTC 3 cut(s) 465, 803, 931
Tru1I TTAA 5 cut(s) 108, 431, 573, 662, 698
Tru9I TTAA 5 cut(s) 108, 431, 573, 662, 698
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 4 cut(s) 214, 354, 793, 827
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 3 cut(s) 666, 761, 969
TspGWI ACGGA 4 cut(s) 203, 307, 725, 932
TspRI CASTG 1 cut(s) 82
VpaK11BI GGWCC 3 cut(s) 304, 315, 373
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 4 cut(s) 145, 521, 611, 733
XhoI CTCGAG 2 cut(s) 224, 873
XspI CTAG 4 cut(s) 257, 389, 684, 1036
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.