Prupe.1G148000_v2.0.a1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0009246
Physical Location & Seq
Forward (+)
11611099 .. 11615405
4307 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G148000.1

Sequence Viewer

Length: 1032 bp
ATGTCGTTCTCGTTCTTCAAGCCGTCGAGGCCCAAAACTCCACAGGAGGTGGCGAAGGCCATCAACGACAGCCTCATGGCCCTCGACACCCAGACCGTCGTCGAAGTTAGAGCCCTCGAGAAGGCTTTGGAAGAAGTTGAAAAGAATTTTGGGACAATGAAGTGTATGGTTTCTGGAGATGGGGAGACCGAACCAAATATGGACCAAGTTTCGCAGCTGGCTCTTGAAATTTGTAAGGAGGGCGTTCTTGATCTTCTGATTCACAAGTTACCTATACTGGGATGGGAAGCAAGAAAAGATTTGGTCCATTGTTGGTCCATATTATTGAAGCAAAAGGTTGACTCTACTTATTGCTGCGCAGAATACATTGAGAACCATTTGGAGTTACTAGACTTTCTTGTCGTGTGCTACGACAACAAGGAGATTGCCTTGAATTGTGGAAATATGTTGAGGGATTGCATTAGATTCCCTACACTTGCAAAATACATATTGGAGTCTGCGAGCTTTGTGTTGTTTTTTAAATTTGTGGAATTGCCTAACTTTGATGTTGCTTCTGATGCGTTCTCTACCTTCAAGGATCTACTAACTAAACATGAAACAGTGGTATCTGAATTTCTGACTGCTCACTATGATGAGTTCTTTGATCTATATGAAAAACTCCTGACATCTCCTAATTATGTGACCAGGAGGCAATCTTTAAAGCTTCTCTCCGAATTCCTTTTGGAGACTCCAAATTCTCATATAATGAAGCGCTACATTCTAGAAGTTCGGTACTTGAAAGTCATGATGACATTGCTCAAGGATTCAAGCAAAAACATCCAAATCTCTGCCTTCCACATCTTTAAGATCTTTGTTGCTAATCCCAATAAGCCACGGGAAGTAAAGGTTATCCTGGCTAAAAACCATGAAAAGTTGACAGAGTTGCTTGAGAATCTCTCTGCCGGCAAAGGTGATGAAGATGAGCAATTTGAAGAGGAAAAGGAGCTGATCATCGCGGAAATCGAAAGAGTAGCCCAACTATCTAATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

344

Amino Acids

39.55

Weight (kDa)

5.17

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
Acc16I TGCGCA 1 cut(s) 358
AccII CGCG 1 cut(s) 995
AciI CCGC 1 cut(s) 995
AclWI GGATC 1 cut(s) 585
AcsI RAATTY 6 cut(s) 145, 228, 521, 611, 713, 733
AfaI GTAC 1 cut(s) 773
AfeI AGCGCT 1 cut(s) 752
AfiI CCNNNNNNNGG 2 cut(s) 121, 278
AgsI TTSAA 9 cut(s) 19, 140, 227, 328, 433, 574, 778, 807, 971
AjnI CCWGG 2 cut(s) 683, 891
AjuI GAANNNNNNNTTGG 2 cut(s) 132, 164
AluBI AGCT 4 cut(s) 217, 504, 703, 985
AluI AGCT 4 cut(s) 217, 504, 703, 985
Alw26I GTCTC 2 cut(s) 179, 719
AlwI GGATC 1 cut(s) 585
Ama87I CYCGRG 1 cut(s) 116
Aor51HI AGCGCT 1 cut(s) 752
AoxI GGCC 3 cut(s) 29, 57, 78
ApeKI GCWGC 2 cut(s) 214, 354
ApoI RAATTY 6 cut(s) 145, 228, 521, 611, 713, 733
AspLEI GCGC 2 cut(s) 359, 753
AspS9I GGNCC 5 cut(s) 30, 79, 202, 304, 315
AsuHPI GGTGA 1 cut(s) 962
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 3 cut(s) 202, 304, 315
BaeI ACNNNNGTAYC 2 cut(s) 588, 621
BanII GRGCYC 1 cut(s) 115
BbvI GCAGC 2 cut(s) 226, 341
BccI CCATC 3 cut(s) 68, 173, 276
BceAI ACGGC 1 cut(s) 7
BciT130I CCWGG 2 cut(s) 685, 893
BclI TGATCA 1 cut(s) 987
BcoDI GTCTC 2 cut(s) 179, 719
BfaI CTAG 2 cut(s) 389, 761
BfoI RGCGCY 1 cut(s) 754
BglI GCCNNNNNGGC 1 cut(s) 28
BglII AGATCT 1 cut(s) 846
BisI GCNGC 2 cut(s) 215, 355
BlsI GCNGC 2 cut(s) 216, 356
Bme1390I CCNGG 2 cut(s) 685, 893
Bme18I GGWCC 3 cut(s) 202, 304, 315
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 5 cut(s) 30, 79, 202, 304, 315
BmrFI CCNGG 2 cut(s) 685, 893
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 1 cut(s) 547
BmuI ACTGGG 1 cut(s) 287
BoxI GACNNNNGTC 1 cut(s) 98
BplI GAGNNNNNCTC 2 cut(s) 920, 952
BpmI CTGGAG 1 cut(s) 195
BpuEI CTTGAG 2 cut(s) 782, 947
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 872
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 278
Bse118I RCCGGY 1 cut(s) 941
Bse1I ACTGG 1 cut(s) 282
Bse3DI GCAATG 1 cut(s) 791
BseBI CCWGG 2 cut(s) 685, 893
BseDI CCNNGG 1 cut(s) 872
BseGI GGATG 2 cut(s) 287, 816
BseLI CCNNNNNNNGG 2 cut(s) 121, 278
BseMI GCAATG 1 cut(s) 791
BseNI ACTGG 1 cut(s) 282
BseXI GCAGC 2 cut(s) 226, 341
Bsh1236I CGCG 1 cut(s) 995
BshFI GGCC 3 cut(s) 31, 59, 80
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 1 cut(s) 942
BslFI GGGAC 1 cut(s) 166
BslI CCNNNNNNNGG 2 cut(s) 121, 278
BsmAI GTCTC 2 cut(s) 179, 719
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 3 cut(s) 31, 59, 80
Bso31I GGTCTC 1 cut(s) 179
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 5 cut(s) 250, 577, 643, 846, 987
BspACI CCGC 1 cut(s) 995
BspANI GGCC 3 cut(s) 31, 59, 80
BspFNI CGCG 1 cut(s) 995
BspHI TCATGA 1 cut(s) 783
BspPI GGATC 1 cut(s) 585
BspTNI GGTCTC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 791
BsrFI RCCGGY 1 cut(s) 941
BsrI ACTGG 1 cut(s) 282
BssAI RCCGGY 1 cut(s) 941
BssECI CCNNGG 1 cut(s) 872
BssMI GATC 5 cut(s) 250, 577, 643, 846, 987
Bst2UI CCWGG 2 cut(s) 685, 893
Bst4CI ACNGT 2 cut(s) 97, 601
Bst6I CTCTTC 1 cut(s) 966
BstC8I GCNNGC 3 cut(s) 219, 502, 943
BstDSI CCRYGG 1 cut(s) 872
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 2 cut(s) 287, 816
BstFNI CGCG 1 cut(s) 995
BstH2I RGCGCY 1 cut(s) 754
BstHHI GCGC 2 cut(s) 359, 753
BstKTI GATC 5 cut(s) 253, 580, 646, 849, 990
BstMAI GTCTC 2 cut(s) 179, 719
BstMBI GATC 5 cut(s) 250, 577, 643, 846, 987
BstMWI GCNNNNNNNGC 2 cut(s) 28, 557
BstNI CCWGG 2 cut(s) 685, 893
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 683, 891
BstUI CGCG 1 cut(s) 995
BstV1I GCAGC 2 cut(s) 226, 341
BstX2I RGATCY 2 cut(s) 577, 846
BstYI RGATCY 2 cut(s) 577, 846
BsuRI GGCC 3 cut(s) 31, 59, 80
BtgI CCRYGG 1 cut(s) 872
BtgZI GCGATG 1 cut(s) 976
BtsCI GGATG 2 cut(s) 287, 816
BtsIMutI CAGTG 1 cut(s) 606
Cac8I GCNNGC 3 cut(s) 219, 502, 943
CciI TCATGA 1 cut(s) 783
CfoI GCGC 2 cut(s) 359, 753
Cfr10I RCCGGY 1 cut(s) 941
Cfr13I GGNCC 5 cut(s) 30, 79, 202, 304, 315
Csp6I GTAC 1 cut(s) 772
CviAII CATG 4 cut(s) 76, 593, 784, 905
CviQI GTAC 1 cut(s) 772
DpnI GATC 5 cut(s) 252, 579, 645, 848, 989
DpnII GATC 5 cut(s) 250, 577, 643, 846, 987
DraI TTTAAA 2 cut(s) 520, 699
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 966
EarI CTCTTC 1 cut(s) 966
Eco24I GRGCYC 1 cut(s) 115
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 3 cut(s) 202, 304, 315
Eco47III AGCGCT 1 cut(s) 752
Eco88I CYCGRG 1 cut(s) 116
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 2 cut(s) 683, 891
EcoT38I GRGCYC 1 cut(s) 115
FaeI CATG 4 cut(s) 79, 596, 787, 908
FaqI GGGAC 1 cut(s) 166
FatI CATG 4 cut(s) 75, 592, 783, 904
FbaI TGATCA 1 cut(s) 987
Fnu4HI GCNGC 2 cut(s) 215, 355
FokI GGATG 2 cut(s) 294, 803
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 2 cut(s) 215, 355
FspBI CTAG 2 cut(s) 389, 761
FspI TGCGCA 1 cut(s) 358
GlaI GCGC 2 cut(s) 358, 752
GluI GCNGC 2 cut(s) 215, 355
GsuI CTGGAG 1 cut(s) 195
HaeII RGCGCY 1 cut(s) 754
HaeIII GGCC 3 cut(s) 31, 59, 80
HapII CCGG 1 cut(s) 942
HhaI GCGC 2 cut(s) 359, 753
Hin1II CATG 4 cut(s) 79, 596, 787, 908
Hin6I GCGC 2 cut(s) 357, 751
HinP1I GCGC 2 cut(s) 357, 751
HincII GTYRAC 2 cut(s) 340, 915
HindII GTYRAC 2 cut(s) 340, 915
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 7 cut(s) 259, 341, 465, 494, 727, 803, 931
HpaII CCGG 1 cut(s) 942
HphI GGTGA 1 cut(s) 962
Hpy166II GTNNAC 2 cut(s) 340, 915
Hpy188I TCNGA 5 cut(s) 258, 556, 610, 618, 712
Hpy188III TCNNGA 7 cut(s) 118, 174, 224, 248, 661, 761, 784
Hpy8I GTNNAC 2 cut(s) 340, 915
Hpy99I CGWCG 3 cut(s) 28, 101, 104
HpyAV CCTTC 4 cut(s) 49, 115, 580, 841
HpyCH4III ACNGT 2 cut(s) 97, 601
HpyCH4V TGCA 2 cut(s) 459, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 557
Hsp92II CATG 4 cut(s) 79, 596, 787, 908
HspAI GCGC 2 cut(s) 357, 751
KroI GCCGGC 1 cut(s) 941
KroNI GCCGGC 1 cut(s) 943
Ksp22I TGATCA 1 cut(s) 987
Kzo9I GATC 5 cut(s) 250, 577, 643, 846, 987
LmnI GCTCC 1 cut(s) 982
Lsp1109I GCAGC 2 cut(s) 226, 341
LweI GCATC 1 cut(s) 547
MaeI CTAG 2 cut(s) 389, 761
MaeIII GTNAC 3 cut(s) 267, 384, 679
MalI GATC 5 cut(s) 252, 579, 645, 848, 989
MboI GATC 5 cut(s) 250, 577, 643, 846, 987
MboII GAAGA 5 cut(s) 7, 143, 245, 968, 983
MflI RGATCY 2 cut(s) 577, 846
MhlI GDGCHC 1 cut(s) 115
MlyI GAGTC 3 cut(s) 335, 503, 721
MnlI CCTC 9 cut(s) 21, 40, 83, 92, 125, 232, 444, 681, 967
MroNI GCCGGC 1 cut(s) 941
MseI TTAA 3 cut(s) 519, 698, 843
MslI CAYNNNNRTG 1 cut(s) 630
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 1 cut(s) 942
MspR9I CCNGG 2 cut(s) 685, 893
MvaI CCWGG 2 cut(s) 685, 893
MvnI CGCG 1 cut(s) 995
MwoI GCNNNNNNNGC 2 cut(s) 28, 557
NaeI GCCGGC 1 cut(s) 943
NdeII GATC 5 cut(s) 250, 577, 643, 846, 987
NgoMIV GCCGGC 1 cut(s) 941
NlaIII CATG 4 cut(s) 79, 596, 787, 908
NmuCI GTSAC 1 cut(s) 679
NsbI TGCGCA 1 cut(s) 358
PaeR7I CTCGAG 1 cut(s) 116
PagI TCATGA 1 cut(s) 783
PcsI WCGNNNNNNNCGW 2 cut(s) 408, 999
PdiI GCCGGC 1 cut(s) 943
PfeI GAWTC 4 cut(s) 259, 465, 803, 931
PkrI GCNGC 2 cut(s) 216, 356
PleI GAGTC 3 cut(s) 335, 502, 721
PpsI GAGTC 3 cut(s) 335, 502, 721
PshAI GACNNNNGTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 683, 891
PspGI CCWGG 2 cut(s) 683, 891
PspPI GGNCC 5 cut(s) 30, 79, 202, 304, 315
PsuI RGATCY 2 cut(s) 577, 846
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 1 cut(s) 630
SaqAI TTAA 3 cut(s) 519, 698, 843
SatI GCNGC 2 cut(s) 215, 355
Sau3AI GATC 5 cut(s) 250, 577, 643, 846, 987
Sau96I GGNCC 5 cut(s) 30, 79, 202, 304, 315
SchI GAGTC 3 cut(s) 335, 503, 721
ScrFI CCNGG 2 cut(s) 685, 893
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 1 cut(s) 547
Sfr274I CTCGAG 1 cut(s) 116
SinI GGWCC 3 cut(s) 202, 304, 315
SlaI CTCGAG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 630
SmlI CTYRAG 3 cut(s) 116, 797, 926
SmoI CTYRAG 3 cut(s) 116, 797, 926
SsiI CCGC 1 cut(s) 995
SspMI CTAG 2 cut(s) 389, 761
StyD4I CCNGG 2 cut(s) 683, 891
TaaI ACNGT 2 cut(s) 97, 601
TaqI TCGA 5 cut(s) 26, 84, 102, 117, 1002
TaqII GACCGA 1 cut(s) 203
TfiI GAWTC 4 cut(s) 259, 465, 803, 931
Tru1I TTAA 3 cut(s) 519, 698, 843
Tru9I TTAA 3 cut(s) 519, 698, 843
TscAI CASTG 1 cut(s) 606
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 2 cut(s) 214, 354
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 6 cut(s) 173, 609, 666, 761, 921, 969
TspRI CASTG 1 cut(s) 606
VpaK11BI GGWCC 3 cut(s) 202, 304, 315
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 6 cut(s) 145, 228, 521, 611, 713, 733
XbaI TCTAGA 1 cut(s) 760
XhoI CTCGAG 1 cut(s) 116
XspI CTAG 2 cut(s) 389, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.