Rh4AG219800

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009246
Physical Location & Seq
Reverse (-)
51759758 .. 51763465
3708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG219800.1

Sequence Viewer

Length: 1182 bp
ATGCTGTTAACGGATCCGCACTGGCACGAAAAAGACCGGGGCTCTTTTCACAATCGTCCTTCTTCACCAAACTCCCAGCTAGCAGCTGTAAATTTCTTCCACAGAGCCAGAGAGAAAGAGAGGGAATTTTATTCCCCATCGATCATGTCGTTCTCGTTCTTCAAGCCTTCGAGGCCCAAAACCCCTCAGGAGGTCGCCAAGGCCATTCACGACAGCCTCACTGCCCTCGACACCCAGACCGTCGCCGAAGTTAAAGCCCTCGAAAAGGCGTTGGAAGAGGTTGAAAAGAATTTCGGGATTATGAGATGTATGCTTGTTGGAGATGCGGAGACAGAACCTAATATGGAACAAGTTTCGCAGCTTGTGCTCGAGATATGTAAGGAGGATGTTCTTGCTCTTCTAGTTCACAAGTTACCTATACTGGGATGGGAAGCAAGAAAAGATTTGGTCCATTGTTGGTCCGTGTTATTGAAGCAAAAGGTTGAGGATACTTATTGCTGCGAACAATACATTGAGGACCATACTGAGTTGCTAGACTTTCTGGTCGTGAGCTATGATAACAAGGAAATTGCCTTAAATTGTGGAGCTATGTTGAGGGATTGCATCAGATTCCCTATTCTTGCAAAATACATATTGGAGTCTGCAAGCTTTGTTTTGTTCTTCAAATTTGTGGAGTTGCCTAACTTTGATGTAGCTTCTGATGCATTTTCTACCTTTAAGGACTTACTTACTAAACATGGAACGGTGGTATCTGAATTTTTGACTGCGCACTATGATGAGTTCTTCGATCTGTATGAAAAACTGTTGACATCTCCTAACTACGTGACTAGGAGGCAATCTTTAAAGCTTCTTACGGATTTCCTATTGGAACCTCCAAATTCTCATATAATGAAACGCTACATTTTAGAAGTTCGGTTCTTGAAAGTCATGATGATGCTGCTGAAGGATTCAAGCAAAAACATCCAGATCGCTGCCTTTCACATTTTCAAGGTCTTTGTTGCTAATCCGAATAAGCCTCGAGAAGTCAAGGTTATTCTGGCTAAGAACCATGCAAAATTGACGGAGTTGCTCCAGAATCTTTCTGCTGGAAAAGGTGATGAAGATGACCAATTTGAAGAGGAAAAGGAGTTAATCATCAAGGAAATTGAATTAGTAACCCAACAACCTAATCTTGGATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

393

Amino Acids

45.43

Weight (kDa)

5.61

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 53 - 383 2.4e-123 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 542
Acc16I TGCGCA 1 cut(s) 768
AciI CCGC 2 cut(s) 17, 326
AclWI GGATC 2 cut(s) 8, 21
AcsI RAATTY 6 cut(s) 91, 125, 289, 665, 755, 877
AcuI CTGAAG 1 cut(s) 962
AfiI CCNNNNNNNGG 4 cut(s) 190, 265, 422, 1172
AgsI TTSAA 9 cut(s) 163, 284, 472, 664, 922, 951, 988, 1115, 1148
AluBI AGCT 8 cut(s) 79, 86, 361, 552, 587, 648, 695, 847
AluI AGCT 8 cut(s) 79, 86, 361, 552, 587, 648, 695, 847
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 1 cut(s) 323
AlwI GGATC 2 cut(s) 8, 21
Ama87I CYCGRG 2 cut(s) 368, 1017
AoxI GGCC 2 cut(s) 173, 201
ApeKI GCWGC 5 cut(s) 83, 358, 498, 937, 971
ApoI RAATTY 6 cut(s) 91, 125, 289, 665, 755, 877
AspLEI GCGC 1 cut(s) 769
AspS9I GGNCC 4 cut(s) 174, 448, 459, 517
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 57, 1106
AsuNHI GCTAGC 1 cut(s) 79
AvaI CYCGRG 2 cut(s) 368, 1017
AvaII GGWCC 3 cut(s) 448, 459, 517
AxyI CCTNAGG 1 cut(s) 186
BaeI ACNNNNGTAYC 2 cut(s) 732, 765
BamHI GGATCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 5 cut(s) 95, 370, 485, 924, 958
BccI CCATC 2 cut(s) 145, 420
BciVI GTATCC 2 cut(s) 481, 1169
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 5 cut(s) 80, 401, 533, 828, 1180
BfuI GTATCC 2 cut(s) 481, 1169
BglI GCCNNNNNGGC 1 cut(s) 172
BisI GCNGC 5 cut(s) 84, 359, 499, 938, 972
BlsI GCNGC 5 cut(s) 85, 360, 500, 939, 973
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 3 cut(s) 448, 459, 517
BmeT110I CYCGRG 2 cut(s) 368, 1017
BmgT120I GGNCC 4 cut(s) 174, 448, 459, 517
BmiI GGNNCC 2 cut(s) 15, 870
BmrFI CCNGG 1 cut(s) 38
BmrI ACTGGG 1 cut(s) 431
BmsI GCATC 4 cut(s) 313, 612, 691, 924
BmtI GCTAGC 1 cut(s) 83
BmuI ACTGGG 1 cut(s) 431
BpmI CTGGAG 1 cut(s) 1055
BpuMI CCSGG 1 cut(s) 38
Bsa29I ATCGAT 1 cut(s) 140
BsaAI YACGTR 1 cut(s) 823
BsaJI CCNNGG 2 cut(s) 37, 198
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 265, 422, 1172
Bse1I ACTGG 2 cut(s) 26, 426
Bse21I CCTNAGG 1 cut(s) 186
BseCI ATCGAT 1 cut(s) 140
BseDI CCNNGG 2 cut(s) 37, 198
BseGI GGATG 3 cut(s) 391, 431, 960
BseLI CCNNNNNNNGG 4 cut(s) 190, 265, 422, 1172
BseMII CTCAG 2 cut(s) 200, 516
BseNI ACTGG 2 cut(s) 26, 426
BseXI GCAGC 5 cut(s) 95, 370, 485, 924, 958
BseYI CCCAGC 1 cut(s) 75
BshFI GGCC 2 cut(s) 175, 203
BshVI ATCGAT 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 2 cut(s) 368, 1017
BsiSI CCGG 1 cut(s) 37
BslI CCNNNNNNNGG 4 cut(s) 190, 265, 422, 1172
BsmAI GTCTC 1 cut(s) 323
BsnI GGCC 2 cut(s) 175, 203
BsoBI CYCGRG 2 cut(s) 368, 1017
Bsp1286I GDGCHC 2 cut(s) 44, 369
Bsp143I GATC 4 cut(s) 13, 141, 787, 966
BspACI CCGC 2 cut(s) 17, 326
BspANI GGCC 2 cut(s) 175, 203
BspCNI CTCAG 2 cut(s) 199, 517
BspDI ATCGAT 1 cut(s) 140
BspHI TCATGA 1 cut(s) 927
BspLI GGNNCC 2 cut(s) 15, 870
BspOI GCTAGC 1 cut(s) 83
BspPI GGATC 2 cut(s) 8, 21
BspQI GCTCTTC 1 cut(s) 402
BsrI ACTGG 2 cut(s) 26, 426
BssECI CCNNGG 2 cut(s) 37, 198
BssMI GATC 4 cut(s) 13, 141, 787, 966
BssT1I CCWWGG 1 cut(s) 198
Bst4CI ACNGT 3 cut(s) 241, 745, 804
Bst6I CTCTTC 3 cut(s) 270, 402, 1110
BstAPI GCANNNNNTGC 1 cut(s) 364
BstBAI YACGTR 1 cut(s) 823
BstC8I GCNNGC 2 cut(s) 81, 646
BstDEI CTNAG 3 cut(s) 186, 525, 1041
BstENI CCTNNNNNAGG 1 cut(s) 263
BstF5I GGATG 3 cut(s) 391, 431, 960
BstHHI GCGC 1 cut(s) 769
BstKTI GATC 4 cut(s) 16, 144, 790, 969
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 4 cut(s) 13, 141, 787, 966
BstMWI GCNNNNNNNGC 3 cut(s) 172, 364, 701
BstSCI CCNGG 1 cut(s) 36
BstV1I GCAGC 5 cut(s) 95, 370, 485, 924, 958
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
Bsu15I ATCGAT 1 cut(s) 140
Bsu36I CCTNAGG 1 cut(s) 186
BsuI GTATCC 2 cut(s) 481, 1169
BsuRI GGCC 2 cut(s) 175, 203
BsuTUI ATCGAT 1 cut(s) 140
BtsCI GGATG 3 cut(s) 391, 431, 960
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 2 cut(s) 19, 219
Cac8I GCNNGC 2 cut(s) 81, 646
CciI TCATGA 1 cut(s) 927
CfoI GCGC 1 cut(s) 769
Cfr13I GGNCC 4 cut(s) 174, 448, 459, 517
ClaI ATCGAT 1 cut(s) 140
CviAII CATG 4 cut(s) 145, 737, 928, 1049
DdeI CTNAG 3 cut(s) 186, 525, 1041
DpnI GATC 4 cut(s) 15, 143, 789, 968
DpnII GATC 4 cut(s) 13, 141, 787, 966
DraI TTTAAA 1 cut(s) 843
DrdI GACNNNNNNGTC 1 cut(s) 542
DseDI GACNNNNNNGTC 1 cut(s) 542
Eam1104I CTCTTC 3 cut(s) 270, 402, 1110
EarI CTCTTC 3 cut(s) 270, 402, 1110
Eco130I CCWWGG 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 3 cut(s) 448, 459, 517
Eco57I CTGAAG 1 cut(s) 962
Eco81I CCTNAGG 1 cut(s) 186
Eco88I CYCGRG 2 cut(s) 368, 1017
EcoNI CCTNNNNNAGG 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 198
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 4 cut(s) 148, 740, 931, 1052
FatI CATG 4 cut(s) 144, 736, 927, 1048
Fnu4HI GCNGC 5 cut(s) 84, 359, 499, 938, 972
FokI GGATG 3 cut(s) 398, 438, 947
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 5 cut(s) 84, 359, 499, 938, 972
FspBI CTAG 5 cut(s) 80, 401, 533, 828, 1180
FspI TGCGCA 1 cut(s) 768
GlaI GCGC 1 cut(s) 768
GluI GCNGC 5 cut(s) 84, 359, 499, 938, 972
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 1055
HaeIII GGCC 2 cut(s) 175, 203
HapII CCGG 1 cut(s) 37
HhaI GCGC 1 cut(s) 769
Hin1II CATG 4 cut(s) 148, 740, 931, 1052
Hin6I GCGC 1 cut(s) 767
HinP1I GCGC 1 cut(s) 767
HincII GTYRAC 2 cut(s) 9, 807
HindII GTYRAC 2 cut(s) 9, 807
HindIII AAGCTT 2 cut(s) 646, 845
HinfI GANTC 4 cut(s) 609, 638, 947, 1075
HpaI GTTAAC 1 cut(s) 9
HpaII CCGG 1 cut(s) 37
HphI GGTGA 2 cut(s) 57, 1106
Hpy166II GTNNAC 3 cut(s) 9, 406, 807
Hpy188I TCNGA 4 cut(s) 608, 700, 754, 1008
Hpy8I GTNNAC 3 cut(s) 9, 406, 807
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 3 cut(s) 69, 177, 937
HpyCH4III ACNGT 3 cut(s) 241, 745, 804
HpyCH4IV ACGT 1 cut(s) 822
HpyCH4V TGCA 5 cut(s) 603, 623, 644, 704, 1052
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 364, 701
HpyF3I CTNAG 3 cut(s) 186, 525, 1041
HpySE526I ACGT 1 cut(s) 822
Hsp92II CATG 4 cut(s) 148, 740, 931, 1052
HspAI GCGC 1 cut(s) 767
KspAI GTTAAC 1 cut(s) 9
Kzo9I GATC 4 cut(s) 13, 141, 787, 966
LguI GCTCTTC 1 cut(s) 402
LmnI GCTCC 2 cut(s) 584, 1074
Lsp1109I GCAGC 5 cut(s) 95, 370, 485, 924, 958
LweI GCATC 4 cut(s) 313, 612, 691, 924
MaeI CTAG 5 cut(s) 80, 401, 533, 828, 1180
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 3 cut(s) 411, 823, 1153
MalI GATC 4 cut(s) 15, 143, 789, 968
MboI GATC 4 cut(s) 13, 141, 787, 966
MboII GAAGA 9 cut(s) 54, 88, 151, 287, 389, 652, 775, 1112, 1127
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 2 cut(s) 44, 369
MlyI GAGTC 1 cut(s) 647
MmeI TCCRAC 2 cut(s) 252, 298
Mph1103I ATGCAT 1 cut(s) 706
MseI TTAA 6 cut(s) 8, 252, 575, 717, 842, 1130
MslI CAYNNNNRTG 2 cut(s) 774, 932
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 172, 364, 701
NciI CCSGG 1 cut(s) 38
NdeII GATC 4 cut(s) 13, 141, 787, 966
NheI GCTAGC 1 cut(s) 79
NlaIII CATG 4 cut(s) 148, 740, 931, 1052
NlaIV GGNNCC 2 cut(s) 15, 870
NmuCI GTSAC 1 cut(s) 823
NsbI TGCGCA 1 cut(s) 768
NsiI ATGCAT 1 cut(s) 706
PaeR7I CTCGAG 2 cut(s) 368, 1017
PagI TCATGA 1 cut(s) 927
PciSI GCTCTTC 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PfeI GAWTC 3 cut(s) 609, 947, 1075
PkrI GCNGC 5 cut(s) 85, 360, 500, 939, 973
PleI GAGTC 1 cut(s) 646
PpsI GAGTC 1 cut(s) 646
Ppu21I YACGTR 1 cut(s) 823
PspFI CCCAGC 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 15, 870
PspPI GGNCC 4 cut(s) 174, 448, 459, 517
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 86
RseI CAYNNNNRTG 2 cut(s) 774, 932
SapI GCTCTTC 1 cut(s) 402
SaqAI TTAA 6 cut(s) 8, 252, 575, 717, 842, 1130
SatI GCNGC 5 cut(s) 84, 359, 499, 938, 972
Sau3AI GATC 4 cut(s) 13, 141, 787, 966
Sau96I GGNCC 4 cut(s) 174, 448, 459, 517
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 2 cut(s) 44, 369
SfaNI GCATC 4 cut(s) 313, 612, 691, 924
Sfr274I CTCGAG 2 cut(s) 368, 1017
SinI GGWCC 3 cut(s) 448, 459, 517
SlaI CTCGAG 2 cut(s) 368, 1017
SmiMI CAYNNNNRTG 2 cut(s) 774, 932
SmlI CTYRAG 2 cut(s) 368, 1017
SmoI CTYRAG 2 cut(s) 368, 1017
SsiI CCGC 2 cut(s) 17, 326
SspMI CTAG 5 cut(s) 80, 401, 533, 828, 1180
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 3 cut(s) 241, 745, 804
TaiI ACGT 1 cut(s) 825
TaqI TCGA 7 cut(s) 140, 170, 228, 261, 369, 786, 1018
TfiI GAWTC 3 cut(s) 609, 947, 1075
Tru1I TTAA 6 cut(s) 8, 252, 575, 717, 842, 1130
Tru9I TTAA 6 cut(s) 8, 252, 575, 717, 842, 1130
TscAI CASTG 2 cut(s) 26, 226
TseFI GTSAC 1 cut(s) 823
TseI GCWGC 5 cut(s) 83, 358, 498, 937, 971
Tsp45I GTSAC 1 cut(s) 823
TspDTI ATGAA 3 cut(s) 810, 905, 1113
TspGWI ACGGA 4 cut(s) 26, 451, 869, 1076
TspRI CASTG 2 cut(s) 26, 226
VpaK11BI GGWCC 3 cut(s) 448, 459, 517
XagI CCTNNNNNAGG 1 cut(s) 263
XapI RAATTY 6 cut(s) 91, 125, 289, 665, 755, 877
XhoI CTCGAG 2 cut(s) 368, 1017
XspI CTAG 5 cut(s) 80, 401, 533, 828, 1180
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.