Rorug04G0158000

BSD domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009246
Physical Location & Seq
Reverse (-)
27544800 .. 27548144
3345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0158000.1

Sequence Viewer

Length: 423 bp
ATGGTTTCCTTCGAGATGAATGATCGGAAAAAGATCGGACTGGGATTGACAGGATTTGGCGTATTTTTCTCATTCCTGGGAATGGTTTTTTTCTTTGACAAGGGATTACTTGCAATGGGAAATATCCTCTTCTTCTCAGGGGTGACTTTAACAATTGGATTTAAGTCCACGCTGCAATTCTTCATGAAACGTCAAAATTTTAAGGGGTCAATTTCTTTTGGTGTTGGGTTCTTCTTTGTGCTCATAGGGTGGCCAATTGTGGGCATGATTTTGGAGGCATATGGTTTCGTTGTACTCTTCAGTGGCTTCTGGCCAACATTGGCAGTTTTTCTGCAGAAGATTCCAATTCTGGGATGGGTGGTCCAACAGCCATATATTAGATCGTTCTTTGACCGATACCGTGGTCGACGGGTGCCTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

140

Amino Acids

15.99

Weight (kDa)

10.54

Isoelectric Point (pI)

26.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 16 - 115 1.1e-13 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 412
AccI GTMKAC 1 cut(s) 406
AcoI YGGCCR 2 cut(s) 251, 311
AcsI RAATTY 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 283
AfaI GTAC 1 cut(s) 294
AfiI CCNNNNNNNGG 3 cut(s) 82, 260, 350
AjnI CCWGG 1 cut(s) 75
Alw21I GWGCWC 1 cut(s) 243
AoxI GGCC 2 cut(s) 251, 311
ApeKI GCWGC 1 cut(s) 172
ApoI RAATTY 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 361
AsuHPI GGTGA 1 cut(s) 154
AvaII GGWCC 1 cut(s) 361
BaeI ACNNNNGTAYC 2 cut(s) 388, 421
BalI TGGCCA 2 cut(s) 253, 313
BanI GGYRCC 1 cut(s) 412
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 159
BccI CCATC 1 cut(s) 348
BciT130I CCWGG 1 cut(s) 77
BfmI CTRYAG 1 cut(s) 332
BisI GCNGC 1 cut(s) 173
BlsI GCNGC 1 cut(s) 174
Bme1390I CCNGG 1 cut(s) 77
Bme18I GGWCC 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 414
BmrFI CCNGG 1 cut(s) 77
BmrI ACTGGG 1 cut(s) 50
BmuI ACTGGG 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 76, 400
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 260, 350
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 120
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 2 cut(s) 76, 400
BseGI GGATG 1 cut(s) 359
BseLI CCNNNNNNNGG 3 cut(s) 82, 260, 350
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 1 cut(s) 45
BseXI GCAGC 1 cut(s) 159
BshFI GGCC 2 cut(s) 253, 313
BshNI GGYRCC 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 243
BslI CCNNNNNNNGG 3 cut(s) 82, 260, 350
BsnI GGCC 2 cut(s) 253, 313
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 3 cut(s) 22, 33, 380
BspANI GGCC 2 cut(s) 253, 313
BspCNI CTCAG 1 cut(s) 149
BspHI TCATGA 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 414
BspMAI CTGCAG 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 412
BsrDI GCAATG 1 cut(s) 120
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 76, 400
BssMI GATC 3 cut(s) 22, 33, 380
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 401
Bst6I CTCTTC 2 cut(s) 134, 302
BstDEI CTNAG 1 cut(s) 136
BstDSI CCRYGG 1 cut(s) 400
BstF5I GGATG 1 cut(s) 359
BstKTI GATC 3 cut(s) 25, 36, 383
BstMBI GATC 3 cut(s) 22, 33, 380
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 75
BstSFI CTRYAG 1 cut(s) 332
BstV1I GCAGC 1 cut(s) 159
BsuRI GGCC 2 cut(s) 253, 313
BtgI CCRYGG 1 cut(s) 400
BtsCI GGATG 1 cut(s) 359
BtsIMutI CAGTG 1 cut(s) 307
CciI TCATGA 1 cut(s) 183
Cfr13I GGNCC 1 cut(s) 361
Csp6I GTAC 1 cut(s) 293
CviAII CATG 2 cut(s) 184, 265
CviJI RGCY 4 cut(s) 253, 306, 313, 370
CviKI_1 RGCY 4 cut(s) 253, 306, 313, 370
CviQI GTAC 1 cut(s) 293
DdeI CTNAG 1 cut(s) 136
DpnI GATC 3 cut(s) 24, 35, 382
DpnII GATC 3 cut(s) 22, 33, 380
EaeI YGGCCR 2 cut(s) 251, 311
Eam1104I CTCTTC 2 cut(s) 134, 302
EarI CTCTTC 2 cut(s) 134, 302
Eco47I GGWCC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 75
FaeI CATG 2 cut(s) 187, 268
FaiI YATR 7 cut(s) 185, 245, 266, 280, 282, 373, 375
FatI CATG 2 cut(s) 183, 264
FauNDI CATATG 1 cut(s) 280
FblI GTMKAC 1 cut(s) 406
Fnu4HI GCNGC 1 cut(s) 173
FokI GGATG 1 cut(s) 366
Fsp4HI GCNGC 1 cut(s) 173
GluI GCNGC 1 cut(s) 173
HaeIII GGCC 2 cut(s) 253, 313
Hin1II CATG 2 cut(s) 187, 268
HincII GTYRAC 1 cut(s) 407
HindII GTYRAC 1 cut(s) 407
HinfI GANTC 1 cut(s) 340
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 2 cut(s) 168, 407
Hpy188I TCNGA 2 cut(s) 27, 38
Hpy188III TCNNGA 2 cut(s) 13, 184
Hpy8I GTNNAC 2 cut(s) 168, 407
Hpy99I CGWCG 1 cut(s) 411
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 1 cut(s) 401
HpyCH4IV ACGT 1 cut(s) 190
HpyCH4V TGCA 3 cut(s) 113, 175, 334
HpyF3I CTNAG 1 cut(s) 136
HpySE526I ACGT 1 cut(s) 190
Hsp92II CATG 2 cut(s) 187, 268
Kzo9I GATC 3 cut(s) 22, 33, 380
LpnPI CCDG 7 cut(s) 26, 36, 62, 89, 123, 295, 335
Lsp1109I GCAGC 1 cut(s) 159
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 1 cut(s) 142
MalI GATC 3 cut(s) 24, 35, 382
MboI GATC 3 cut(s) 22, 33, 380
MboII GAAGA 6 cut(s) 121, 124, 172, 223, 289, 349
MfeI CAATTG 2 cut(s) 153, 255
MhlI GDGCHC 1 cut(s) 243
MlsI TGGCCA 2 cut(s) 253, 313
MluCI AATT 6 cut(s) 153, 176, 196, 210, 255, 345
MluNI TGGCCA 2 cut(s) 253, 313
MmeI TCCRAC 1 cut(s) 388
MnlI CCTC 2 cut(s) 137, 268
Mox20I TGGCCA 2 cut(s) 253, 313
MscI TGGCCA 2 cut(s) 253, 313
MseI TTAA 3 cut(s) 149, 162, 201
Msp20I TGGCCA 2 cut(s) 253, 313
MspR9I CCNGG 1 cut(s) 77
MunI CAATTG 2 cut(s) 153, 255
MvaI CCWGG 1 cut(s) 77
NdeI CATATG 1 cut(s) 280
NdeII GATC 3 cut(s) 22, 33, 380
NlaIII CATG 2 cut(s) 187, 268
NlaIV GGNNCC 1 cut(s) 414
NmuCI GTSAC 1 cut(s) 142
PagI TCATGA 1 cut(s) 183
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 1 cut(s) 361
PstI CTGCAG 1 cut(s) 336
RsaI GTAC 1 cut(s) 294
RsaNI GTAC 1 cut(s) 293
SalI GTCGAC 1 cut(s) 405
SaqAI TTAA 3 cut(s) 149, 162, 201
SatI GCNGC 1 cut(s) 173
Sau3AI GATC 3 cut(s) 22, 33, 380
Sau96I GGNCC 1 cut(s) 361
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 1 cut(s) 243
SetI ASST 1 cut(s) 193
SfcI CTRYAG 1 cut(s) 332
SinI GGWCC 1 cut(s) 361
Sse9I AATT 6 cut(s) 153, 176, 196, 210, 255, 345
StyD4I CCNGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 401
TaiI ACGT 1 cut(s) 193
TaqI TCGA 2 cut(s) 12, 406
TaqII GACCGA 1 cut(s) 408
TasI AATT 6 cut(s) 153, 176, 196, 210, 255, 345
TatI WGTACW 1 cut(s) 292
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 3 cut(s) 149, 162, 201
Tru9I TTAA 3 cut(s) 149, 162, 201
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 3 cut(s) 32, 172, 200
TspRI CASTG 1 cut(s) 307
VpaK11BI GGWCC 1 cut(s) 361
XapI RAATTY 1 cut(s) 196
XcmI CCANNNNNNNNNTGG 1 cut(s) 351
XmiI GTMKAC 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.