MD13G1179400.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009246
Physical Location & Seq
Forward (+)
14953333 .. 14957587
4255 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1179400.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGTCGTTCTCGTTCTTCAAGCCGTCGAGGCCCAAAACCCCACAGGAGGTGGCCAAGGCCATCAACGACAGCCTGTCAGCCCTCGACACCCAGACCGTCGTCGAAGTCAAAGCCCTCGAAAAGGCTTTGGAAGAAGTTGAAAAGAATTTCACAACAATGAAATGTTTGCTTTCTGGAGATGGGGAGACCGAACCAAATATGGATCAAGTTTCGCAGCTTGCGCTCGAGATTTGTAAGGAGGGTGTTTTTGACCTTCTAATTCACAAGTTACCTATACTGGGATGGGAAGCAAGAAAGGATTTGGTCCATTGTTGGTCCATATTGATGAAGCAAAAGGTTGAATTGACTTATTGCTGCGCAGAATACATGGAGAACCATTTGGAATTATTAGACTTTCTTGTCGTGTGCTATGATAACAAGGAAATTGCTTTGAATTGTGGAAGTATGTTGAGGGATTGCATTAGATTCCCTGCACTTGCAAAATACATACTGGAGTCTGCGAGCTTTGAGTTGTTTTTTAAATTTGTGGAGCTGCCTAACTTTGATGTTGCTTCTGATGCATTCTCTACCTTTAAGGATCTACTTACTAAACATGGAACGGTGGTATCTGAATTTTTGACTGCTCACTATGATGAGTTCTTTGATCTATATGAGAAGCTCTTGACATCTCCAAATTATGTGACCAGGAGGCAATCTTTGAAGCTTCTCTCCGAATTCCTCTTGGAGACTCCAAATTCACATATAATGAAGCGCTACATCTTAGAAGTTCGGTACTTGAAAGTCATGATGACGTTGCTCAAGGATTCAAGTAAAAACATCCAAATCTCTGCCTTCCACATCTTCAAGGTCTTTGTTGCGAATCCTAATAAGCCACGGGAAGTAAAACTTATCCTGGCTAAAAACCATGAAAAGTTGACGGACTTGCTTCAGAATCTTTCTGCTGGAAAAGGCGATGAAGACGAGCAATTTGAAGAGGAAAAGGAGCTGATCATCAAGGAAATCGAAGGAGTATATCAGCTAGTTAATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

344

Amino Acids

39.54

Weight (kDa)

5.22

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 5 - 335 7.3e-126 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
Acc16I TGCGCA 1 cut(s) 358
AclWI GGATC 2 cut(s) 210, 585
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 5 cut(s) 145, 521, 611, 713, 733
AcuI CTGAAG 1 cut(s) 911
AfaI GTAC 1 cut(s) 773
AfeI AGCGCT 1 cut(s) 752
AfiI CCNNNNNNNGG 3 cut(s) 46, 121, 278
AgsI TTSAA 9 cut(s) 19, 140, 341, 433, 700, 778, 807, 844, 971
AhdI GACNNNNNGTC 1 cut(s) 73
AjnI CCWGG 2 cut(s) 683, 891
AluBI AGCT 7 cut(s) 217, 504, 532, 658, 703, 985, 1018
AluI AGCT 7 cut(s) 217, 504, 532, 658, 703, 985, 1018
Alw26I GTCTC 2 cut(s) 179, 719
AlwI GGATC 2 cut(s) 210, 585
Ama87I CYCGRG 1 cut(s) 224
Aor51HI AGCGCT 1 cut(s) 752
AoxI GGCC 3 cut(s) 29, 51, 57
ApeKI GCWGC 3 cut(s) 214, 354, 532
ApoI RAATTY 5 cut(s) 145, 521, 611, 713, 733
ArsI GACNNNNNNTTYG 2 cut(s) 950, 982
AspLEI GCGC 3 cut(s) 223, 359, 753
AspS9I GGNCC 3 cut(s) 30, 304, 315
AvaI CYCGRG 1 cut(s) 224
AvaII GGWCC 2 cut(s) 304, 315
BaeI ACNNNNGTAYC 2 cut(s) 588, 621
BalI TGGCCA 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 963
BbvI GCAGC 3 cut(s) 226, 341, 519
BccI CCATC 3 cut(s) 68, 173, 276
BceAI ACGGC 1 cut(s) 7
BciT130I CCWGG 2 cut(s) 685, 893
BclI TGATCA 1 cut(s) 987
BcoDI GTCTC 2 cut(s) 179, 719
BfaI CTAG 1 cut(s) 1019
BfmI CTRYAG 1 cut(s) 1028
BfoI RGCGCY 1 cut(s) 754
BglI GCCNNNNNGGC 1 cut(s) 28
BisI GCNGC 3 cut(s) 215, 355, 533
BlsI GCNGC 3 cut(s) 216, 356, 534
Bme1390I CCNGG 2 cut(s) 685, 893
Bme18I GGWCC 2 cut(s) 304, 315
BmeRI GACNNNNNGTC 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 224
BmgT120I GGNCC 3 cut(s) 30, 304, 315
BmrFI CCNGG 2 cut(s) 685, 893
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 1 cut(s) 547
BmuI ACTGGG 1 cut(s) 287
BoxI GACNNNNGTC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 963
BpmI CTGGAG 2 cut(s) 195, 512
BpuEI CTTGAG 1 cut(s) 782
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 54, 872
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 121, 278
Bse1I ACTGG 2 cut(s) 282, 495
BseBI CCWGG 2 cut(s) 685, 893
BseDI CCNNGG 2 cut(s) 54, 872
BseGI GGATG 2 cut(s) 287, 816
BseLI CCNNNNNNNGG 3 cut(s) 46, 121, 278
BseNI ACTGG 2 cut(s) 282, 495
BseXI GCAGC 3 cut(s) 226, 341, 519
BsgI GTGCAG 1 cut(s) 456
BshFI GGCC 3 cut(s) 31, 53, 59
BsiHKCI CYCGRG 1 cut(s) 224
BslI CCNNNNNNNGG 3 cut(s) 46, 121, 278
BsmAI GTCTC 2 cut(s) 179, 719
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 3 cut(s) 31, 53, 59
Bso31I GGTCTC 1 cut(s) 179
BsoBI CYCGRG 1 cut(s) 224
Bsp143I GATC 4 cut(s) 202, 577, 643, 987
BspANI GGCC 3 cut(s) 31, 53, 59
BspHI TCATGA 1 cut(s) 783
BspPI GGATC 2 cut(s) 210, 585
BspTNI GGTCTC 1 cut(s) 179
BsrI ACTGG 2 cut(s) 282, 495
BssECI CCNNGG 2 cut(s) 54, 872
BssMI GATC 4 cut(s) 202, 577, 643, 987
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 2 cut(s) 685, 893
Bst4CI ACNGT 2 cut(s) 97, 601
Bst6I CTCTTC 1 cut(s) 966
BstC8I GCNNGC 2 cut(s) 219, 502
BstDEI CTNAG 1 cut(s) 760
BstDSI CCRYGG 1 cut(s) 872
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 2 cut(s) 287, 816
BstH2I RGCGCY 1 cut(s) 754
BstHHI GCGC 3 cut(s) 223, 359, 753
BstKTI GATC 4 cut(s) 205, 580, 646, 990
BstMAI GTCTC 2 cut(s) 179, 719
BstMBI GATC 4 cut(s) 202, 577, 643, 987
BstMWI GCNNNNNNNGC 3 cut(s) 28, 220, 557
BstNI CCWGG 2 cut(s) 685, 893
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 683, 891
BstSFI CTRYAG 1 cut(s) 1028
BstV1I GCAGC 3 cut(s) 226, 341, 519
BstV2I GAAGAC 1 cut(s) 963
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BsuRI GGCC 3 cut(s) 31, 53, 59
BtgI CCRYGG 1 cut(s) 872
BtgZI GCGATG 1 cut(s) 966
BtsCI GGATG 2 cut(s) 287, 816
Cac8I GCNNGC 2 cut(s) 219, 502
CciI TCATGA 1 cut(s) 783
CfoI GCGC 3 cut(s) 223, 359, 753
Cfr13I GGNCC 3 cut(s) 30, 304, 315
Csp6I GTAC 1 cut(s) 772
CviAII CATG 4 cut(s) 367, 593, 784, 905
CviQI GTAC 1 cut(s) 772
DdeI CTNAG 1 cut(s) 760
DpnI GATC 4 cut(s) 204, 579, 645, 989
DpnII GATC 4 cut(s) 202, 577, 643, 987
DraI TTTAAA 1 cut(s) 520
DrdI GACNNNNNNGTC 1 cut(s) 398
DriI GACNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 398
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 966
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 966
Eco130I CCWWGG 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 2 cut(s) 304, 315
Eco47III AGCGCT 1 cut(s) 752
Eco57I CTGAAG 1 cut(s) 911
Eco88I CYCGRG 1 cut(s) 224
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 2 cut(s) 683, 891
EcoT14I CCWWGG 1 cut(s) 54
EcoT22I ATGCAT 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 4 cut(s) 370, 596, 787, 908
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FatI CATG 4 cut(s) 366, 592, 783, 904
FbaI TGATCA 1 cut(s) 987
Fnu4HI GCNGC 3 cut(s) 215, 355, 533
FokI GGATG 2 cut(s) 294, 803
Fsp4HI GCNGC 3 cut(s) 215, 355, 533
FspBI CTAG 1 cut(s) 1019
FspI TGCGCA 1 cut(s) 358
GlaI GCGC 3 cut(s) 222, 358, 752
GluI GCNGC 3 cut(s) 215, 355, 533
GsuI CTGGAG 2 cut(s) 195, 512
HaeII RGCGCY 1 cut(s) 754
HaeIII GGCC 3 cut(s) 31, 53, 59
HhaI GCGC 3 cut(s) 223, 359, 753
Hin1II CATG 4 cut(s) 370, 596, 787, 908
Hin6I GCGC 3 cut(s) 221, 357, 751
HinP1I GCGC 3 cut(s) 221, 357, 751
HincII GTYRAC 1 cut(s) 915
HindII GTYRAC 1 cut(s) 915
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 6 cut(s) 465, 494, 727, 803, 859, 931
Hpy166II GTNNAC 1 cut(s) 915
Hpy188I TCNGA 4 cut(s) 556, 610, 712, 930
Hpy188III TCNNGA 4 cut(s) 174, 226, 661, 784
Hpy8I GTNNAC 1 cut(s) 915
Hpy99I CGWCG 3 cut(s) 28, 101, 104
HpyAV CCTTC 3 cut(s) 263, 841, 998
HpyCH4III ACNGT 2 cut(s) 97, 601
HpyCH4IV ACGT 1 cut(s) 791
HpyCH4V TGCA 4 cut(s) 459, 473, 479, 560
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 220, 557
HpyF3I CTNAG 1 cut(s) 760
HpySE526I ACGT 1 cut(s) 791
Hsp92II CATG 4 cut(s) 370, 596, 787, 908
HspAI GCGC 3 cut(s) 221, 357, 751
Ksp22I TGATCA 1 cut(s) 987
Kzo9I GATC 4 cut(s) 202, 577, 643, 987
LmnI GCTCC 2 cut(s) 529, 982
Lsp1109I GCAGC 3 cut(s) 226, 341, 519
LweI GCATC 1 cut(s) 547
MaeI CTAG 1 cut(s) 1019
MaeII ACGT 1 cut(s) 791
MaeIII GTNAC 2 cut(s) 267, 679
MalI GATC 4 cut(s) 204, 579, 645, 989
MboI GATC 4 cut(s) 202, 577, 643, 987
MboII GAAGA 5 cut(s) 7, 143, 832, 968, 983
MflI RGATCY 1 cut(s) 577
MlsI TGGCCA 1 cut(s) 53
MluNI TGGCCA 1 cut(s) 53
MlyI GAGTC 2 cut(s) 503, 721
MnlI CCTC 9 cut(s) 21, 40, 92, 125, 232, 444, 681, 728, 967
Mox20I TGGCCA 1 cut(s) 53
Mph1103I ATGCAT 1 cut(s) 562
MscI TGGCCA 1 cut(s) 53
MseI TTAA 3 cut(s) 519, 573, 1023
MslI CAYNNNNRTG 3 cut(s) 155, 323, 630
Msp20I TGGCCA 1 cut(s) 53
MspR9I CCNGG 2 cut(s) 685, 893
Mva1269I GAATGC 1 cut(s) 560
MvaI CCWGG 2 cut(s) 685, 893
MwoI GCNNNNNNNGC 3 cut(s) 28, 220, 557
NdeII GATC 4 cut(s) 202, 577, 643, 987
NlaIII CATG 4 cut(s) 370, 596, 787, 908
NmuCI GTSAC 1 cut(s) 679
NsbI TGCGCA 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 562
PaeR7I CTCGAG 1 cut(s) 224
PagI TCATGA 1 cut(s) 783
PctI GAATGC 1 cut(s) 560
PfeI GAWTC 4 cut(s) 465, 803, 859, 931
PkrI GCNGC 3 cut(s) 216, 356, 534
PleI GAGTC 2 cut(s) 502, 721
PpsI GAGTC 2 cut(s) 502, 721
PshAI GACNNNNGTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 683, 891
PspGI CCWGG 2 cut(s) 683, 891
PspPI GGNCC 3 cut(s) 30, 304, 315
PsuI RGATCY 1 cut(s) 577
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 3 cut(s) 155, 323, 630
SaqAI TTAA 3 cut(s) 519, 573, 1023
SatI GCNGC 3 cut(s) 215, 355, 533
Sau3AI GATC 4 cut(s) 202, 577, 643, 987
Sau96I GGNCC 3 cut(s) 30, 304, 315
SchI GAGTC 2 cut(s) 503, 721
ScrFI CCNGG 2 cut(s) 685, 893
SfaNI GCATC 1 cut(s) 547
SfcI CTRYAG 1 cut(s) 1028
Sfr274I CTCGAG 1 cut(s) 224
SinI GGWCC 2 cut(s) 304, 315
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 3 cut(s) 155, 323, 630
SmlI CTYRAG 2 cut(s) 224, 797
SmoI CTYRAG 2 cut(s) 224, 797
SspMI CTAG 1 cut(s) 1019
StyD4I CCNGG 2 cut(s) 683, 891
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 2 cut(s) 97, 601
TaiI ACGT 1 cut(s) 794
TaqI TCGA 6 cut(s) 26, 84, 102, 117, 225, 1002
TaqII GACCGA 1 cut(s) 203
TfiI GAWTC 4 cut(s) 465, 803, 859, 931
Tru1I TTAA 3 cut(s) 519, 573, 1023
Tru9I TTAA 3 cut(s) 519, 573, 1023
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 3 cut(s) 214, 354, 532
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 5 cut(s) 173, 341, 761, 921, 969
TspGWI ACGGA 1 cut(s) 932
VpaK11BI GGWCC 2 cut(s) 304, 315
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 5 cut(s) 145, 521, 611, 713, 733
XhoI CTCGAG 1 cut(s) 224
XspI CTAG 1 cut(s) 1019
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.