Rh4BG221300

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009246
Physical Location & Seq
Reverse (-)
38202595 .. 38206807
4213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG221300.1

Sequence Viewer

Length: 837 bp
ATGCTGTTAACGGATCCGCACTGGCACGAAAAAGACCGGGGCTCTTTTCCCAATCGTCCTTCTTCACCAAACTCACAGCTAGCAGCTGTAAATTTCTTCCACAGAGAAAGAGACAGAGCCAGAGAGAAAGAGTGGGAATTTGATTCCCCATCGATCATGTCGTTCTCGTTCTTCAAGCCTTCAAGGCCCAAAACCCCTCAGGAGGTCGCCAAGGCCATTCACGACAGCCTCACTGCCCTCGACACCCAGACCGTCGCCGAAGTTAAAGCCCTCGAAAAGGCGTTGGAAGAGGTTGAAAAGAATTTCGGGATTATGAGATGTATGCTTGTTGGAGATGCGGAGACAGAACCTAATATGGAACAAGTTTCGCAGCTTGTGCTCGAGATATGTAAGGAGGATGTTCTTGCTCTTCTAGTTCACAAGTTACCTATACTGGGATGGGAAGCAAGAAAAGATTTGGTCCATTGTTGGTCCGTGTTATTGAAGCAAAAGGTTGAGGATACTTATTGCTGCGAACAATACATTGAGGACCATACTGAGTTGCTAGACTTTCTGGTCGTGAGCTATGATAACAAGGAAATTGCCTTAAATTGTGGAGCTATGTTGAGGGATTGCATCAGATTCCCTATTCTTGCAAAATACATATTGGAGTCTGCAAGCTTTGTTTTGTTCTTCAAATTTGTGGAGTTGCCTAACTTTGATGTAGCTTCTGATGCATTTTCTACCTTTAAGGACTTGCTTACTAAACATGGAACGGTGGTATCTGAATTTTTGACTGCGCACTATGATGAGGTTTGCTCAAACTTTGTCTTAATATATTTTTCTGTAATAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

278

Amino Acids

31.97

Weight (kDa)

5.05

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 57 - 269 1.4e-66 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
Acc16I TGCGCA 1 cut(s) 780
AciI CCGC 2 cut(s) 17, 338
AclWI GGATC 2 cut(s) 8, 21
AcsI RAATTY 5 cut(s) 91, 137, 301, 677, 767
AfiI CCNNNNNNNGG 3 cut(s) 202, 277, 434
AgsI TTSAA 5 cut(s) 175, 183, 296, 484, 676
AluBI AGCT 7 cut(s) 79, 86, 373, 564, 599, 660, 707
AluI AGCT 7 cut(s) 79, 86, 373, 564, 599, 660, 707
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 2 cut(s) 105, 335
AlwI GGATC 2 cut(s) 8, 21
Ama87I CYCGRG 1 cut(s) 380
AoxI GGCC 2 cut(s) 185, 213
ApeKI GCWGC 3 cut(s) 83, 370, 510
ApoI RAATTY 5 cut(s) 91, 137, 301, 677, 767
AspLEI GCGC 1 cut(s) 781
AspS9I GGNCC 4 cut(s) 186, 460, 471, 529
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 1 cut(s) 57
AsuNHI GCTAGC 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 3 cut(s) 460, 471, 529
AxyI CCTNAGG 1 cut(s) 198
BaeI ACNNNNGTAYC 2 cut(s) 744, 777
BamHI GGATCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 3 cut(s) 95, 382, 497
BccI CCATC 2 cut(s) 157, 432
BciVI GTATCC 1 cut(s) 493
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 2 cut(s) 105, 335
BfaI CTAG 4 cut(s) 80, 413, 545, 835
BfuI GTATCC 1 cut(s) 493
BglI GCCNNNNNGGC 1 cut(s) 184
BisI GCNGC 3 cut(s) 84, 371, 511
BlsI GCNGC 3 cut(s) 85, 372, 512
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 3 cut(s) 460, 471, 529
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 4 cut(s) 186, 460, 471, 529
BmiI GGNNCC 1 cut(s) 15
BmrFI CCNGG 1 cut(s) 38
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 3 cut(s) 325, 624, 703
BmtI GCTAGC 1 cut(s) 83
BmuI ACTGGG 1 cut(s) 443
BplI GAGNNNNNCTC 2 cut(s) 782, 814
BpuMI CCSGG 1 cut(s) 38
Bsa29I ATCGAT 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 37, 210
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 277, 434
Bse1I ACTGG 2 cut(s) 26, 438
Bse21I CCTNAGG 1 cut(s) 198
BseCI ATCGAT 1 cut(s) 152
BseDI CCNNGG 2 cut(s) 37, 210
BseGI GGATG 2 cut(s) 403, 443
BseLI CCNNNNNNNGG 3 cut(s) 202, 277, 434
BseMII CTCAG 2 cut(s) 212, 528
BseNI ACTGG 2 cut(s) 26, 438
BseXI GCAGC 3 cut(s) 95, 382, 497
BshFI GGCC 2 cut(s) 187, 215
BshVI ATCGAT 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 1 cut(s) 37
BslI CCNNNNNNNGG 3 cut(s) 202, 277, 434
BsmAI GTCTC 2 cut(s) 105, 335
BsnI GGCC 2 cut(s) 187, 215
BsoBI CYCGRG 1 cut(s) 380
Bsp1286I GDGCHC 2 cut(s) 44, 381
Bsp143I GATC 2 cut(s) 13, 153
BspACI CCGC 2 cut(s) 17, 338
BspANI GGCC 2 cut(s) 187, 215
BspCNI CTCAG 2 cut(s) 211, 529
BspDI ATCGAT 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 15
BspOI GCTAGC 1 cut(s) 83
BspPI GGATC 2 cut(s) 8, 21
BspQI GCTCTTC 1 cut(s) 414
BsrI ACTGG 2 cut(s) 26, 438
BssECI CCNNGG 2 cut(s) 37, 210
BssMI GATC 2 cut(s) 13, 153
BssT1I CCWWGG 1 cut(s) 210
Bst4CI ACNGT 2 cut(s) 253, 757
Bst6I CTCTTC 2 cut(s) 282, 414
BstAPI GCANNNNNTGC 1 cut(s) 376
BstC8I GCNNGC 2 cut(s) 81, 658
BstDEI CTNAG 2 cut(s) 198, 537
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 2 cut(s) 403, 443
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 2 cut(s) 16, 156
BstMAI GTCTC 2 cut(s) 105, 335
BstMBI GATC 2 cut(s) 13, 153
BstMWI GCNNNNNNNGC 3 cut(s) 184, 376, 713
BstSCI CCNGG 1 cut(s) 36
BstV1I GCAGC 3 cut(s) 95, 382, 497
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
Bsu15I ATCGAT 1 cut(s) 152
Bsu36I CCTNAGG 1 cut(s) 198
BsuI GTATCC 1 cut(s) 493
BsuRI GGCC 2 cut(s) 187, 215
BsuTUI ATCGAT 1 cut(s) 152
BtsCI GGATG 2 cut(s) 403, 443
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 2 cut(s) 19, 231
Cac8I GCNNGC 2 cut(s) 81, 658
CfoI GCGC 1 cut(s) 781
Cfr13I GGNCC 4 cut(s) 186, 460, 471, 529
ClaI ATCGAT 1 cut(s) 152
CviAII CATG 2 cut(s) 157, 749
DdeI CTNAG 2 cut(s) 198, 537
DpnI GATC 2 cut(s) 15, 155
DpnII GATC 2 cut(s) 13, 153
DrdI GACNNNNNNGTC 1 cut(s) 554
DseDI GACNNNNNNGTC 1 cut(s) 554
Eam1104I CTCTTC 2 cut(s) 282, 414
EarI CTCTTC 2 cut(s) 282, 414
Eco130I CCWWGG 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 3 cut(s) 460, 471, 529
Eco81I CCTNAGG 1 cut(s) 198
Eco88I CYCGRG 1 cut(s) 380
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoT14I CCWWGG 1 cut(s) 210
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 2 cut(s) 160, 752
FatI CATG 2 cut(s) 156, 748
Fnu4HI GCNGC 3 cut(s) 84, 371, 511
FokI GGATG 2 cut(s) 410, 450
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 3 cut(s) 84, 371, 511
FspBI CTAG 4 cut(s) 80, 413, 545, 835
FspI TGCGCA 1 cut(s) 780
GlaI GCGC 1 cut(s) 780
GluI GCNGC 3 cut(s) 84, 371, 511
HaeIII GGCC 2 cut(s) 187, 215
HapII CCGG 1 cut(s) 37
HhaI GCGC 1 cut(s) 781
Hin1II CATG 2 cut(s) 160, 752
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HindIII AAGCTT 1 cut(s) 658
HinfI GANTC 3 cut(s) 143, 621, 650
HpaI GTTAAC 1 cut(s) 9
HpaII CCGG 1 cut(s) 37
HphI GGTGA 1 cut(s) 57
Hpy166II GTNNAC 2 cut(s) 9, 418
Hpy188I TCNGA 3 cut(s) 620, 712, 766
Hpy188III TCNNGA 5 cut(s) 200, 221, 307, 382, 559
Hpy8I GTNNAC 2 cut(s) 9, 418
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 2 cut(s) 69, 189
HpyCH4III ACNGT 2 cut(s) 253, 757
HpyCH4V TGCA 4 cut(s) 615, 635, 656, 716
HpyF10VI GCNNNNNNNGC 3 cut(s) 184, 376, 713
HpyF3I CTNAG 2 cut(s) 198, 537
Hsp92II CATG 2 cut(s) 160, 752
HspAI GCGC 1 cut(s) 779
KspAI GTTAAC 1 cut(s) 9
Kzo9I GATC 2 cut(s) 13, 153
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 596
LpnPI CCDG 7 cut(s) 7, 50, 133, 185, 260, 419, 539
Lsp1109I GCAGC 3 cut(s) 95, 382, 497
LweI GCATC 3 cut(s) 325, 624, 703
MaeI CTAG 4 cut(s) 80, 413, 545, 835
MaeIII GTNAC 1 cut(s) 423
MalI GATC 2 cut(s) 15, 155
MboI GATC 2 cut(s) 13, 153
MboII GAAGA 6 cut(s) 54, 88, 163, 299, 401, 664
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 2 cut(s) 44, 381
MluCI AATT 7 cut(s) 91, 137, 301, 579, 589, 677, 767
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 2 cut(s) 264, 310
Mph1103I ATGCAT 1 cut(s) 718
MseI TTAA 5 cut(s) 8, 264, 587, 729, 812
MslI CAYNNNNRTG 1 cut(s) 786
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 184, 376, 713
NciI CCSGG 1 cut(s) 38
NdeII GATC 2 cut(s) 13, 153
NheI GCTAGC 1 cut(s) 79
NlaIII CATG 2 cut(s) 160, 752
NlaIV GGNNCC 1 cut(s) 15
NsbI TGCGCA 1 cut(s) 780
NsiI ATGCAT 1 cut(s) 718
PaeR7I CTCGAG 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PfeI GAWTC 2 cut(s) 143, 621
PkrI GCNGC 3 cut(s) 85, 372, 512
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
PspN4I GGNNCC 1 cut(s) 15
PspPI GGNCC 4 cut(s) 186, 460, 471, 529
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 86
RseI CAYNNNNRTG 1 cut(s) 786
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 5 cut(s) 8, 264, 587, 729, 812
SatI GCNGC 3 cut(s) 84, 371, 511
Sau3AI GATC 2 cut(s) 13, 153
Sau96I GGNCC 4 cut(s) 186, 460, 471, 529
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 2 cut(s) 44, 381
SfaNI GCATC 3 cut(s) 325, 624, 703
Sfr274I CTCGAG 1 cut(s) 380
SinI GGWCC 3 cut(s) 460, 471, 529
SlaI CTCGAG 1 cut(s) 380
SmiMI CAYNNNNRTG 1 cut(s) 786
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 7 cut(s) 91, 137, 301, 579, 589, 677, 767
SsiI CCGC 2 cut(s) 17, 338
SspMI CTAG 4 cut(s) 80, 413, 545, 835
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 2 cut(s) 253, 757
TaqI TCGA 4 cut(s) 152, 240, 273, 381
TasI AATT 7 cut(s) 91, 137, 301, 579, 589, 677, 767
TfiI GAWTC 2 cut(s) 143, 621
Tru1I TTAA 5 cut(s) 8, 264, 587, 729, 812
Tru9I TTAA 5 cut(s) 8, 264, 587, 729, 812
TscAI CASTG 2 cut(s) 26, 238
TseI GCWGC 3 cut(s) 83, 370, 510
TspGWI ACGGA 2 cut(s) 26, 463
TspRI CASTG 2 cut(s) 26, 238
VpaK11BI GGWCC 3 cut(s) 460, 471, 529
XagI CCTNNNNNAGG 1 cut(s) 275
XapI RAATTY 5 cut(s) 91, 137, 301, 677, 767
XhoI CTCGAG 1 cut(s) 380
XspI CTAG 4 cut(s) 80, 413, 545, 835
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.