Rh4CG232600

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009246
Physical Location & Seq
Reverse (-)
47142932 .. 47147479
4548 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG232600.1

Sequence Viewer

Length: 969 bp
ATGCTGTTAACGGATCCGCACTGGCACGAAAAAGACCGGGGCTCTTTTCACAATCGTCCTTCTTCACCAAACTCCCAGCTAGCAGCTGTAAATTTCTTCCACAGAGAGAGAGAAAGAGCTAGAGAGAGGGAATTTGATTCCCCATCGATCATGTCGTTCTCGTTCTTCAAGCCGTCGAGGCCCAAAACCCCTCAGGAGGTCGCCAAGGCCATTCACGACAGCCTCACTGCCCTCGACACCCAGACCGTCGCCGAAGTTAAAGCCCTCGAAAAGGCGTTGGAAGAGGTTGAAAAGAATTTCGGGATTATGAGATGTATGCTTGTTGGAGATGCGGAGACAGAACCTAATATGGAACAAGTTTCGCAGCTTGTGCTCGAGATATGTAAGGAGGATGTTCTTGCTCTTCTAGTTCACAAGTTACCTATACTGGGATGGGAAGCAAGAAAAGATTTGGTCCATTGTTGGTCCGTGTTATTGAAGCAAAAGGTTGAGGATACTTATTGCTGCGAACAATACATTGAGGACCATACTGAGTTGCTAGACTTTCTGGTCGTGAGCTATGATAACAAGGAAATTGCCTTAAATTGTGGAGCTATGTTGAGGGATTGCATCAGATTCCCTATTCTTGCAAAATACATATTGGAGTCTGCAAGCTTTGTTTTGTTCTTCAAATTTGTGGAGTTGCCTAACTTTGATGTAGCTTCTGATGCATTTTCTACGTTTAAGGACTTACTTACTAAACATGGAATGGTGGTATCTGAATTTTTGACTGCGCACTATGATGAGTTCTTCGATCTGTATGAAAAACTGTTGACATCTCCTAACTACGTGACTAGGAGGCAATCTTTAAAGCTTCTTACGGATTTCCTATTGGAGCCTCCAAATTCTCATATAATGAAACGCTACATTTTAGAAGTTCGGTTCTTGAAAGTCATGATGATGCTGCTGAAGGTATTGACTTTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

322

Amino Acids

37.5

Weight (kDa)

5.62

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 55 - 317 6.3e-91 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
Acc16I TGCGCA 1 cut(s) 774
AciI CCGC 2 cut(s) 17, 332
AclWI GGATC 2 cut(s) 8, 21
AcsI RAATTY 6 cut(s) 91, 131, 295, 671, 761, 883
AcuI CTGAAG 1 cut(s) 968
AfiI CCNNNNNNNGG 3 cut(s) 196, 271, 428
AgsI TTSAA 5 cut(s) 169, 290, 478, 670, 928
AluBI AGCT 9 cut(s) 79, 86, 119, 367, 558, 593, 654, 701, 853
AluI AGCT 9 cut(s) 79, 86, 119, 367, 558, 593, 654, 701, 853
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 1 cut(s) 329
AlwI GGATC 2 cut(s) 8, 21
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 2 cut(s) 179, 207
ApeKI GCWGC 4 cut(s) 83, 364, 504, 943
ApoI RAATTY 6 cut(s) 91, 131, 295, 671, 761, 883
AspLEI GCGC 1 cut(s) 775
AspS9I GGNCC 4 cut(s) 180, 454, 465, 523
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 1 cut(s) 57
AsuNHI GCTAGC 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 3 cut(s) 454, 465, 523
AxyI CCTNAGG 1 cut(s) 192
BamHI GGATCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 375
BbvI GCAGC 4 cut(s) 95, 376, 491, 930
BccI CCATC 2 cut(s) 151, 426
BceAI ACGGC 1 cut(s) 157
BciVI GTATCC 1 cut(s) 487
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 5 cut(s) 80, 120, 407, 539, 834
BfuI GTATCC 1 cut(s) 487
BglI GCCNNNNNGGC 1 cut(s) 178
BisI GCNGC 4 cut(s) 84, 365, 505, 944
BlsI GCNGC 4 cut(s) 85, 366, 506, 945
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 3 cut(s) 454, 465, 523
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 4 cut(s) 180, 454, 465, 523
BmiI GGNNCC 2 cut(s) 15, 876
BmrFI CCNGG 1 cut(s) 38
BmrI ACTGGG 1 cut(s) 437
BmsI GCATC 4 cut(s) 319, 618, 697, 930
BmtI GCTAGC 1 cut(s) 83
BmuI ACTGGG 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 38
Bsa29I ATCGAT 1 cut(s) 146
BsaAI YACGTR 1 cut(s) 829
BsaJI CCNNGG 2 cut(s) 37, 204
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 271, 428
Bse1I ACTGG 2 cut(s) 26, 432
Bse21I CCTNAGG 1 cut(s) 192
BseCI ATCGAT 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 37, 204
BseGI GGATG 2 cut(s) 397, 437
BseLI CCNNNNNNNGG 3 cut(s) 196, 271, 428
BseMII CTCAG 2 cut(s) 206, 522
BseNI ACTGG 2 cut(s) 26, 432
BseXI GCAGC 4 cut(s) 95, 376, 491, 930
BseYI CCCAGC 1 cut(s) 75
BshFI GGCC 2 cut(s) 181, 209
BshVI ATCGAT 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 1 cut(s) 37
BslI CCNNNNNNNGG 3 cut(s) 196, 271, 428
BsmAI GTCTC 1 cut(s) 329
BsnI GGCC 2 cut(s) 181, 209
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 44, 375
Bsp143I GATC 3 cut(s) 13, 147, 793
BspACI CCGC 2 cut(s) 17, 332
BspANI GGCC 2 cut(s) 181, 209
BspCNI CTCAG 2 cut(s) 205, 523
BspDI ATCGAT 1 cut(s) 146
BspHI TCATGA 1 cut(s) 933
BspLI GGNNCC 2 cut(s) 15, 876
BspOI GCTAGC 1 cut(s) 83
BspPI GGATC 2 cut(s) 8, 21
BspQI GCTCTTC 1 cut(s) 408
BsrI ACTGG 2 cut(s) 26, 432
BssECI CCNNGG 2 cut(s) 37, 204
BssMI GATC 3 cut(s) 13, 147, 793
BssT1I CCWWGG 1 cut(s) 204
Bst4CI ACNGT 2 cut(s) 247, 810
Bst6I CTCTTC 2 cut(s) 276, 408
BstAPI GCANNNNNTGC 1 cut(s) 370
BstBAI YACGTR 1 cut(s) 829
BstC8I GCNNGC 2 cut(s) 81, 652
BstDEI CTNAG 2 cut(s) 192, 531
BstENI CCTNNNNNAGG 1 cut(s) 269
BstF5I GGATG 2 cut(s) 397, 437
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 3 cut(s) 16, 150, 796
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 3 cut(s) 13, 147, 793
BstMWI GCNNNNNNNGC 3 cut(s) 178, 370, 707
BstSCI CCNGG 1 cut(s) 36
BstV1I GCAGC 4 cut(s) 95, 376, 491, 930
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
Bsu15I ATCGAT 1 cut(s) 146
Bsu36I CCTNAGG 1 cut(s) 192
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 2 cut(s) 181, 209
BsuTUI ATCGAT 1 cut(s) 146
BtsCI GGATG 2 cut(s) 397, 437
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 2 cut(s) 19, 225
Cac8I GCNNGC 2 cut(s) 81, 652
CciI TCATGA 1 cut(s) 933
CfoI GCGC 1 cut(s) 775
Cfr13I GGNCC 4 cut(s) 180, 454, 465, 523
ClaI ATCGAT 1 cut(s) 146
CviAII CATG 3 cut(s) 151, 743, 934
DdeI CTNAG 2 cut(s) 192, 531
DpnI GATC 3 cut(s) 15, 149, 795
DpnII GATC 3 cut(s) 13, 147, 793
DraI TTTAAA 1 cut(s) 849
DrdI GACNNNNNNGTC 1 cut(s) 548
DseDI GACNNNNNNGTC 1 cut(s) 548
Eam1104I CTCTTC 2 cut(s) 276, 408
EarI CTCTTC 2 cut(s) 276, 408
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 3 cut(s) 454, 465, 523
Eco57I CTGAAG 1 cut(s) 968
Eco81I CCTNAGG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 374
EcoNI CCTNNNNNAGG 1 cut(s) 269
EcoT14I CCWWGG 1 cut(s) 204
EcoT22I ATGCAT 1 cut(s) 712
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 3 cut(s) 154, 746, 937
FatI CATG 3 cut(s) 150, 742, 933
Fnu4HI GCNGC 4 cut(s) 84, 365, 505, 944
FokI GGATG 2 cut(s) 404, 444
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 4 cut(s) 84, 365, 505, 944
FspBI CTAG 5 cut(s) 80, 120, 407, 539, 834
FspI TGCGCA 1 cut(s) 774
GlaI GCGC 1 cut(s) 774
GluI GCNGC 4 cut(s) 84, 365, 505, 944
GsaI CCCAGC 1 cut(s) 79
HaeIII GGCC 2 cut(s) 181, 209
HapII CCGG 1 cut(s) 37
HhaI GCGC 1 cut(s) 775
Hin1II CATG 3 cut(s) 154, 746, 937
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HincII GTYRAC 2 cut(s) 9, 813
HindII GTYRAC 2 cut(s) 9, 813
HindIII AAGCTT 2 cut(s) 652, 851
HinfI GANTC 3 cut(s) 137, 615, 644
HpaI GTTAAC 1 cut(s) 9
HpaII CCGG 1 cut(s) 37
HphI GGTGA 1 cut(s) 57
Hpy166II GTNNAC 3 cut(s) 9, 412, 813
Hpy188I TCNGA 3 cut(s) 614, 706, 760
Hpy188III TCNNGA 7 cut(s) 194, 215, 301, 376, 553, 925, 934
Hpy8I GTNNAC 3 cut(s) 9, 412, 813
Hpy99I CGWCG 2 cut(s) 178, 251
HpyAV CCTTC 2 cut(s) 69, 943
HpyCH4III ACNGT 2 cut(s) 247, 810
HpyCH4IV ACGT 2 cut(s) 719, 828
HpyCH4V TGCA 4 cut(s) 609, 629, 650, 710
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 370, 707
HpyF3I CTNAG 2 cut(s) 192, 531
HpySE526I ACGT 2 cut(s) 719, 828
Hsp92II CATG 3 cut(s) 154, 746, 937
HspAI GCGC 1 cut(s) 773
KspAI GTTAAC 1 cut(s) 9
Kzo9I GATC 3 cut(s) 13, 147, 793
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 2 cut(s) 590, 874
LpnPI CCDG 7 cut(s) 7, 50, 89, 179, 254, 413, 533
Lsp1109I GCAGC 4 cut(s) 95, 376, 491, 930
LweI GCATC 4 cut(s) 319, 618, 697, 930
MaeI CTAG 5 cut(s) 80, 120, 407, 539, 834
MaeII ACGT 2 cut(s) 719, 828
MaeIII GTNAC 2 cut(s) 417, 829
MalI GATC 3 cut(s) 15, 149, 795
MboI GATC 3 cut(s) 13, 147, 793
MboII GAAGA 7 cut(s) 54, 88, 157, 293, 395, 658, 781
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 2 cut(s) 44, 375
MluCI AATT 8 cut(s) 91, 131, 295, 573, 583, 671, 761, 883
MlyI GAGTC 1 cut(s) 653
MmeI TCCRAC 2 cut(s) 258, 304
Mph1103I ATGCAT 1 cut(s) 712
MseI TTAA 5 cut(s) 8, 258, 581, 723, 848
MslI CAYNNNNRTG 2 cut(s) 780, 938
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 178, 370, 707
NciI CCSGG 1 cut(s) 38
NdeII GATC 3 cut(s) 13, 147, 793
NheI GCTAGC 1 cut(s) 79
NlaIII CATG 3 cut(s) 154, 746, 937
NlaIV GGNNCC 2 cut(s) 15, 876
NmuCI GTSAC 1 cut(s) 829
NsbI TGCGCA 1 cut(s) 774
NsiI ATGCAT 1 cut(s) 712
PaeR7I CTCGAG 1 cut(s) 374
PagI TCATGA 1 cut(s) 933
PciSI GCTCTTC 1 cut(s) 408
PcsI WCGNNNNNNNCGW 1 cut(s) 152
PfeI GAWTC 2 cut(s) 137, 615
PkrI GCNGC 4 cut(s) 85, 366, 506, 945
PleI GAGTC 1 cut(s) 652
PpsI GAGTC 1 cut(s) 652
Ppu21I YACGTR 1 cut(s) 829
PspFI CCCAGC 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 15, 876
PspPI GGNCC 4 cut(s) 180, 454, 465, 523
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 86
RseI CAYNNNNRTG 2 cut(s) 780, 938
SapI GCTCTTC 1 cut(s) 408
SaqAI TTAA 5 cut(s) 8, 258, 581, 723, 848
SatI GCNGC 4 cut(s) 84, 365, 505, 944
Sau3AI GATC 3 cut(s) 13, 147, 793
Sau96I GGNCC 4 cut(s) 180, 454, 465, 523
SchI GAGTC 1 cut(s) 653
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 2 cut(s) 44, 375
SfaNI GCATC 4 cut(s) 319, 618, 697, 930
Sfr274I CTCGAG 1 cut(s) 374
SinI GGWCC 3 cut(s) 454, 465, 523
SlaI CTCGAG 1 cut(s) 374
SmiMI CAYNNNNRTG 2 cut(s) 780, 938
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 8 cut(s) 91, 131, 295, 573, 583, 671, 761, 883
SsiI CCGC 2 cut(s) 17, 332
SspMI CTAG 5 cut(s) 80, 120, 407, 539, 834
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 2 cut(s) 247, 810
TaiI ACGT 2 cut(s) 722, 831
TaqI TCGA 6 cut(s) 146, 176, 234, 267, 375, 792
TasI AATT 8 cut(s) 91, 131, 295, 573, 583, 671, 761, 883
TfiI GAWTC 2 cut(s) 137, 615
Tru1I TTAA 5 cut(s) 8, 258, 581, 723, 848
Tru9I TTAA 5 cut(s) 8, 258, 581, 723, 848
TscAI CASTG 2 cut(s) 26, 232
TseFI GTSAC 1 cut(s) 829
TseI GCWGC 4 cut(s) 83, 364, 504, 943
Tsp45I GTSAC 1 cut(s) 829
TspDTI ATGAA 2 cut(s) 816, 911
TspGWI ACGGA 3 cut(s) 26, 457, 875
TspRI CASTG 2 cut(s) 26, 232
VpaK11BI GGWCC 3 cut(s) 454, 465, 523
XagI CCTNNNNNAGG 1 cut(s) 269
XapI RAATTY 6 cut(s) 91, 131, 295, 671, 761, 883
XhoI CTCGAG 1 cut(s) 374
XspI CTAG 5 cut(s) 80, 120, 407, 539, 834
Zsp2I ATGCAT 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.