FvH4_4g17000

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005136
Physical Location & Seq
Forward (+)
20902320 .. 20907249
4930 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17000.t2

Sequence Viewer

Length: 1284 bp
ATGCAAGCGACTCAAGGAGTGAGTAATGGAGTTACAGAAGCTCCTGCTGCTCAGGCTGGTGCTGAGAATCTGAAGTCCATACAGGAAGAAGGTGGAGAATCGGGACATAGTGGAAGACGCCCTGATATATCACTTCAAATACCCCCAAGGCATGCAGGGTTTGGCAGTAGCCGAAGCGGGAAGGGTTTACTCCAGTCTCAAGGTTCCTCTAAGGGTAGTCCACTATCAGGAGGCTTGTTCCGGGCTTTAAGCTTCAAGAAGAAAGGCACATTGCCGGATGGTGAGAGAAGTTCCCTCCTTAATTCAGACTCCAATACAGCTCCTGGAAGTCCAGTAGTGAACAATAATGGACCAGCATTTTCTTGGACAAAGTGTGCCTCTCTTCCTGTTACACCAGCATCAAATATCTCACCATCCATATCTACACCTGCTTCTGCAAGGACGTATAACGAAAGGCACAAGGCACATAAAGAGAATGTGAAGCCTACAGTTTCAAGGTCTCTGTCCGTGCCTGGACGGAATATTGTTATTGTCAGATCCATATCTTTTTCTGCTCGAAATGAGAACATCGAAACAGATCCTAGTGATGATCAAATAACTCCGGTTCCAGTCGAGGCTGAAGATGAAGAAATTCCTGAAGAAGAAGCAGTATGTAGGATTTGTTTTGATGAATGTGAAGAACAAAACACTCTCAAAATGGAATGCAGTTGCAAAGGTGCTCTCAGACTTGTACATGAACAGTGTGCAATTAAGTGGTTTAGCACAAGAGGAAACAGGACATGTGAGGTATGTGGGCAGGAGGTTCAGAATTTACCTGTAACCTTGCTGCGGGTGCAGAGTTCTGTTCAAAGGGCCAGCGGACAGAACCAAAATCAACAGAGTCTGCATCCAGAAACAATAAGTGCATGGCAGGACTTCGTAATACTGGTATTAATTAGCACAATATGCTATTTCTTCTTCCTTGAGCAACTACTGATCCGGGACTTGAAAGCTCAAGCTATTGTAGTTGCAGCACCATTTGCATTTACATTGGGTCTCTTAGCATCCATATTCGCCATTGTCCTTGCAATCAAGGAGTATATATGGACATATGCAGCTCTCGAGTTTGGCCTTGTAGCACTCTCCGTGCACCTTTTCTATTCTACATTTCATCTGCCAGCCATATATTCAATACTGCTTGCATCAGTAGTGGGTTTTGGGATATCAATGAGCCTCAACTCATTGTATATGCAGTACTTCAGTTGGAGAGTTCAAGTTGCTCAGAACAACTCTAGCCCAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

428

Amino Acids

46.34

Weight (kDa)

6.27

Isoelectric Point (pI)

55.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 218 - 264 1.6e-14 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 436
Acc36I ACCTGC 1 cut(s) 436
AciI CCGC 3 cut(s) 177, 829, 858
AclWI GGATC 3 cut(s) 531, 572, 970
AcsI RAATTY 2 cut(s) 630, 808
AcuI CTGAAG 4 cut(s) 92, 639, 657, 1222
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 2 cut(s) 732, 1235
AfiI CCNNNNNNNGG 2 cut(s) 227, 828
AflIII ACRYGT 1 cut(s) 779
AgsI TTSAA 7 cut(s) 137, 256, 495, 848, 988, 1170, 1253
AjnI CCWGG 2 cut(s) 322, 511
AluBI AGCT 6 cut(s) 41, 252, 320, 992, 998, 1097
AluI AGCT 6 cut(s) 41, 252, 320, 992, 998, 1097
Alw21I GWGCWC 2 cut(s) 721, 1131
Alw26I GTCTC 3 cut(s) 201, 504, 1040
Alw44I GTGCAC 1 cut(s) 1127
AlwI GGATC 3 cut(s) 531, 572, 970
AlwNI CAGNNNCTG 2 cut(s) 323, 883
Ama87I CYCGRG 1 cut(s) 1100
AoxI GGCC 2 cut(s) 852, 1108
ApaLI GTGCAC 1 cut(s) 1127
ApeKI GCWGC 4 cut(s) 47, 826, 1010, 1094
ApoI RAATTY 2 cut(s) 630, 808
AseI ATTAAT 1 cut(s) 932
Asp700I GAANNNNTTC 1 cut(s) 630
AspS9I GGNCC 2 cut(s) 350, 852
AsuC2I CCSGG 2 cut(s) 242, 980
AsuHPI GGTGA 2 cut(s) 293, 402
AvaI CYCGRG 1 cut(s) 1100
AvaII GGWCC 1 cut(s) 350
BaeGI GKGCMC 1 cut(s) 1131
BbsI GAAGAC 1 cut(s) 121
Bbv12I GWGCWC 2 cut(s) 721, 1131
BbvI GCAGC 4 cut(s) 34, 813, 1022, 1106
BccI CCATC 2 cut(s) 272, 421
BciT130I CCWGG 2 cut(s) 324, 513
BclI TGATCA 1 cut(s) 589
BcnI CCSGG 2 cut(s) 242, 980
BcoDI GTCTC 3 cut(s) 201, 504, 1040
BfaI CTAG 2 cut(s) 582, 1272
BfmI CTRYAG 1 cut(s) 486
BfuAI ACCTGC 1 cut(s) 436
BisI GCNGC 4 cut(s) 48, 827, 1011, 1095
BlsI GCNGC 4 cut(s) 49, 828, 1012, 1096
BmcAI AGTACT 1 cut(s) 1235
Bme1390I CCNGG 4 cut(s) 242, 324, 513, 980
Bme18I GGWCC 1 cut(s) 350
BmeT110I CYCGRG 1 cut(s) 1100
BmgT120I GGNCC 2 cut(s) 350, 852
BmiI GGNNCC 2 cut(s) 205, 606
BmrFI CCNGG 4 cut(s) 242, 324, 513, 980
BmrI ACTGGG 1 cut(s) 1271
BmsI GCATC 4 cut(s) 407, 895, 1052, 1190
BmuI ACTGGG 1 cut(s) 1271
BpiI GAAGAC 1 cut(s) 121
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 1 cut(s) 51
BpuEI CTTGAG 3 cut(s) 183, 978, 983
BpuMI CCSGG 2 cut(s) 242, 980
BsaBI GATNNNNATC 1 cut(s) 541
BsaHI GRCGYC 1 cut(s) 118
BsaI GGTCTC 2 cut(s) 504, 1040
BsaJI CCNNGG 1 cut(s) 146
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 2 cut(s) 25, 55
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 828
Bse1I ACTGG 5 cut(s) 193, 332, 608, 930, 1277
Bse3DI GCAATG 1 cut(s) 269
Bse8I GATNNNNATC 1 cut(s) 541
BseBI CCWGG 2 cut(s) 324, 513
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 4 cut(s) 283, 413, 886, 1043
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 2 cut(s) 227, 828
BseMI GCAATG 1 cut(s) 269
BseMII CTCAG 4 cut(s) 54, 65, 736, 1274
BseNI ACTGG 5 cut(s) 193, 332, 608, 930, 1277
BseSI GKGCMC 1 cut(s) 1131
BseXI GCAGC 4 cut(s) 34, 813, 1022, 1106
BsgI GTGCAG 1 cut(s) 854
BshFI GGCC 2 cut(s) 854, 1110
BsiHKAI GWGCWC 2 cut(s) 721, 1131
BsiHKCI CYCGRG 1 cut(s) 1100
BsiSI CCGG 4 cut(s) 241, 275, 602, 979
BslFI GGGAC 2 cut(s) 117, 995
BslI CCNNNNNNNGG 2 cut(s) 227, 828
BsmAI GTCTC 3 cut(s) 201, 504, 1040
BsmFI GGGAC 2 cut(s) 117, 995
BsmI GAATGC 1 cut(s) 707
BsnI GGCC 2 cut(s) 854, 1110
Bso31I GGTCTC 2 cut(s) 504, 1040
BsoBI CYCGRG 1 cut(s) 1100
Bsp1286I GDGCHC 2 cut(s) 721, 1131
Bsp1407I TGTACA 1 cut(s) 730
Bsp143I GATC 4 cut(s) 536, 577, 589, 975
BspACI CCGC 3 cut(s) 177, 829, 858
BspANI GGCC 2 cut(s) 854, 1110
BspCNI CTCAG 4 cut(s) 55, 64, 735, 1273
BspLI GGNNCC 2 cut(s) 205, 606
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 3 cut(s) 531, 572, 970
BspTNI GGTCTC 2 cut(s) 504, 1040
BsrDI GCAATG 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 730
BsrI ACTGG 5 cut(s) 193, 332, 608, 930, 1277
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 4 cut(s) 536, 577, 589, 975
BssNI GRCGYC 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 2 cut(s) 324, 513
Bst4CI ACNGT 2 cut(s) 490, 741
Bst6I CTCTTC 1 cut(s) 387
BstACI GRCGYC 1 cut(s) 118
BstAPI GCANNNNNTGC 2 cut(s) 945, 1019
BstAUI TGTACA 1 cut(s) 730
BstC8I GCNNGC 5 cut(s) 6, 153, 856, 1158, 1179
BstDEI CTNAG 6 cut(s) 51, 63, 210, 722, 1039, 1260
BstF5I GGATG 4 cut(s) 283, 413, 886, 1043
BstKTI GATC 4 cut(s) 539, 580, 592, 978
BstMAI GTCTC 3 cut(s) 201, 504, 1040
BstMBI GATC 4 cut(s) 536, 577, 589, 975
BstMWI GCNNNNNNNGC 5 cut(s) 47, 53, 832, 945, 1019
BstNI CCWGG 2 cut(s) 324, 513
BstNSI RCATGY 2 cut(s) 155, 783
BstSCI CCNGG 4 cut(s) 240, 322, 511, 978
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 1131
BstV1I GCAGC 4 cut(s) 34, 813, 1022, 1106
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 2 cut(s) 536, 577
BstYI RGATCY 2 cut(s) 536, 577
BsuRI GGCC 2 cut(s) 854, 1110
BtsCI GGATG 4 cut(s) 283, 413, 886, 1043
BtsIMutI CAGTG 1 cut(s) 746
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 5 cut(s) 6, 153, 856, 1158, 1179
CaiI CAGNNNCTG 2 cut(s) 323, 883
Cfr13I GGNCC 2 cut(s) 350, 852
CseI GACGC 1 cut(s) 126
Csp6I GTAC 2 cut(s) 731, 1234
CviAII CATG 4 cut(s) 152, 734, 780, 906
CviQI GTAC 2 cut(s) 731, 1234
DdeI CTNAG 6 cut(s) 51, 63, 210, 722, 1039, 1260
DpnI GATC 4 cut(s) 538, 579, 591, 977
DpnII GATC 4 cut(s) 536, 577, 589, 975
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 146
Eco31I GGTCTC 2 cut(s) 504, 1040
Eco32I GATATC 1 cut(s) 1203
Eco47I GGWCC 1 cut(s) 350
Eco57I CTGAAG 4 cut(s) 92, 639, 657, 1222
Eco88I CYCGRG 1 cut(s) 1100
EcoRII CCWGG 2 cut(s) 322, 511
EcoRV GATATC 1 cut(s) 1203
EcoT14I CCWWGG 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 4 cut(s) 155, 737, 783, 909
FaqI GGGAC 2 cut(s) 117, 995
FatI CATG 4 cut(s) 151, 733, 779, 905
FauI CCCGC 2 cut(s) 170, 822
FauNDI CATATG 1 cut(s) 1090
FbaI TGATCA 1 cut(s) 589
Fnu4HI GCNGC 4 cut(s) 48, 827, 1011, 1095
FokI GGATG 4 cut(s) 290, 400, 873, 1030
Fsp4HI GCNGC 4 cut(s) 48, 827, 1011, 1095
FspBI CTAG 2 cut(s) 582, 1272
GluI GCNGC 4 cut(s) 48, 827, 1011, 1095
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 2 cut(s) 854, 1110
HapII CCGG 4 cut(s) 241, 275, 602, 979
HgaI GACGC 1 cut(s) 126
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 4 cut(s) 155, 737, 783, 909
HindIII AAGCTT 1 cut(s) 250
HinfI GANTC 5 cut(s) 10, 67, 98, 308, 880
HpaII CCGG 4 cut(s) 241, 275, 602, 979
HphI GGTGA 2 cut(s) 293, 402
Hpy166II GTNNAC 4 cut(s) 188, 221, 340, 1129
Hpy188I TCNGA 6 cut(s) 72, 307, 536, 725, 807, 1263
Hpy188III TCNNGA 6 cut(s) 102, 228, 256, 635, 890, 1100
Hpy8I GTNNAC 4 cut(s) 188, 221, 340, 1129
HpyAV CCTTC 2 cut(s) 83, 175
HpyCH4III ACNGT 2 cut(s) 490, 741
HpyCH4IV ACGT 1 cut(s) 443
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 53, 832, 945, 1019
HpyF3I CTNAG 6 cut(s) 51, 63, 210, 722, 1039, 1260
HpySE526I ACGT 1 cut(s) 443
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 4 cut(s) 155, 737, 783, 909
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 4 cut(s) 536, 577, 589, 975
LmnI GCTCC 2 cut(s) 46, 325
Lsp1109I GCAGC 4 cut(s) 34, 813, 1022, 1106
LweI GCATC 4 cut(s) 407, 895, 1052, 1190
MaeI CTAG 2 cut(s) 582, 1272
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 3 cut(s) 31, 388, 817
MalI GATC 4 cut(s) 538, 579, 591, 977
MboI GATC 4 cut(s) 536, 577, 589, 975
MflI RGATCY 2 cut(s) 536, 577
MhlI GDGCHC 2 cut(s) 721, 1131
MluCI AATT 5 cut(s) 301, 630, 747, 808, 933
MlyI GAGTC 3 cut(s) 4, 302, 889
MmeI TCCRAC 1 cut(s) 1223
MnlI CCTC 9 cut(s) 217, 224, 305, 388, 607, 761, 778, 793, 1223
MroXI GAANNNNTTC 1 cut(s) 630
MseI TTAA 4 cut(s) 248, 300, 750, 932
MspA1I CMGCKG 1 cut(s) 858
MspI CCGG 4 cut(s) 241, 275, 602, 979
MspR9I CCNGG 4 cut(s) 242, 324, 513, 980
Mva1269I GAATGC 1 cut(s) 707
MvaI CCWGG 2 cut(s) 324, 513
MwoI GCNNNNNNNGC 5 cut(s) 47, 53, 832, 945, 1019
NciI CCSGG 2 cut(s) 242, 980
NdeI CATATG 1 cut(s) 1090
NdeII GATC 4 cut(s) 536, 577, 589, 975
NlaIII CATG 4 cut(s) 155, 737, 783, 909
NlaIV GGNNCC 2 cut(s) 205, 606
NspI RCATGY 2 cut(s) 155, 783
PaeI GCATGC 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 1100
PaqCI CACCTGC 1 cut(s) 436
PciI ACATGT 1 cut(s) 779
PctI GAATGC 1 cut(s) 707
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 67, 98
PfoI TCCNGGA 2 cut(s) 322, 978
PkrI GCNGC 4 cut(s) 49, 828, 1012, 1096
PleI GAGTC 3 cut(s) 4, 302, 888
PpsI GAGTC 3 cut(s) 4, 302, 888
PscI ACATGT 1 cut(s) 779
PshBI ATTAAT 1 cut(s) 932
Psp6I CCWGG 2 cut(s) 322, 511
PspGI CCWGG 2 cut(s) 322, 511
PspN4I GGNNCC 2 cut(s) 205, 606
PspPI GGNCC 2 cut(s) 350, 852
PstNI CAGNNNCTG 2 cut(s) 323, 883
PsuI RGATCY 2 cut(s) 536, 577
RsaI GTAC 2 cut(s) 732, 1235
RsaNI GTAC 2 cut(s) 731, 1234
SaqAI TTAA 4 cut(s) 248, 300, 750, 932
SatI GCNGC 4 cut(s) 48, 827, 1011, 1095
Sau3AI GATC 4 cut(s) 536, 577, 589, 975
Sau96I GGNCC 2 cut(s) 350, 852
ScaI AGTACT 1 cut(s) 1235
SchI GAGTC 3 cut(s) 4, 302, 889
ScrFI CCNGG 4 cut(s) 242, 324, 513, 980
SduI GDGCHC 2 cut(s) 721, 1131
SfaNI GCATC 4 cut(s) 407, 895, 1052, 1190
SfcI CTRYAG 1 cut(s) 486
Sfr274I CTCGAG 1 cut(s) 1100
SinI GGWCC 1 cut(s) 350
SlaI CTCGAG 1 cut(s) 1100
SmlI CTYRAG 5 cut(s) 12, 198, 962, 993, 1100
SmoI CTYRAG 5 cut(s) 12, 198, 962, 993, 1100
SphI GCATGC 1 cut(s) 155
Sse9I AATT 5 cut(s) 301, 630, 747, 808, 933
SsiI CCGC 3 cut(s) 177, 829, 858
SspI AATATT 1 cut(s) 523
SspMI CTAG 2 cut(s) 582, 1272
StyD4I CCNGG 4 cut(s) 240, 322, 511, 978
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 2 cut(s) 490, 741
TaiI ACGT 1 cut(s) 446
TaqI TCGA 4 cut(s) 556, 570, 612, 1101
TasI AATT 5 cut(s) 301, 630, 747, 808, 933
TatI WGTACW 2 cut(s) 730, 1233
TfiI GAWTC 2 cut(s) 67, 98
Tru1I TTAA 4 cut(s) 248, 300, 750, 932
Tru9I TTAA 4 cut(s) 248, 300, 750, 932
TscAI CASTG 1 cut(s) 746
TseI GCWGC 4 cut(s) 47, 826, 1010, 1094
TspDTI ATGAA 4 cut(s) 639, 684, 750, 1139
TspGWI ACGGA 3 cut(s) 496, 532, 1114
TspRI CASTG 1 cut(s) 746
VneI GTGCAC 1 cut(s) 1127
VpaK11BI GGWCC 1 cut(s) 350
VspI ATTAAT 1 cut(s) 932
XapI RAATTY 2 cut(s) 630, 808
XceI RCATGY 2 cut(s) 155, 783
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XhoI CTCGAG 1 cut(s) 1100
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 2 cut(s) 582, 1272
ZrmI AGTACT 1 cut(s) 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.