MD16G1176800.v1.1

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005136
Physical Location & Seq
Reverse (-)
15044691 .. 15049142
4452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1176800.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGCAAGCTGCTCAATCAGTCTCCAACGGAGCTGCAGAACGTCCTCCTTCTCCTCAGGCTGATTTCCAAAATCTGAAGACAGTAAATGAAGAAGCTACCGACCTGGAACCTACTGGGAGGCGGCCAGACATATCACTTCAGATACCCCGAAGGCCTGTGGGTTTTGGCAGTAGCCGTAGTGGAAAGGGTTTACTGCAGTCTCAAGGTTCCTTTAAGGGCAGCTCGCTATCAGGGGGCCTTTTCCGGGGTTTAAGCTTCAAGAAAAAAGGCAATCAACCAGATGGTGAGAGAAGCTCCCTCCTTAATTCGGACCCCAGTAGAGCTCCTGAAAGTTGTACCTCTCTTCCTTTTACACCGATATCAAATTTGTCCCCCTCTGTAGCTACACCCAGCTCTGCGAGGATGTATAATGAAGTGCACAAGCCACATAAAGAAACTACAACAGTTTCGAGGTCCCTTTCCATGCCTGGACGTAATAAAGTCATTGTAAGATCCGTATCTTTTGCAACTCGAAATGAGCAGACTCAAGCAGAACCTAGTGATGATCAAATAACTCCAGTTCCAGTGGAGGCTAATGATGAAGAAATTCCTGAAGAAGAAGCAGTGTGCAGGATATGTCTTGATGTATGTGATGAACAAAATACGCTCAAAATGGAATGTCTTTGCAAAGGTGCTCTTAGACTTCTACATGAAGAGTGCGCCATTAAGTGGTTCAGCACAAAAGGAAACAAGAAATGTGATGTTTGTGGTCAAGAGGTTCAGAATTTACCTGTAACATTGCTTCGGGTGCAGAGCACTGCTCAAAGGGGTAGTGGACAGCAGCACAACCAACAGGGTTTGCATCCAGAAACGATAAGTGCCTGGCAGGACTTTGTGGTAATGGTCTTGATTAGCACAATATGCTATTTCTTCTTCCTTGAGCAGCTACTGATTCGTGACTTGAAAACTAGAGCGATTGTAATTACAGCACCGTTTGCATTTACATTGGGTCTCTTGGCATCCACTTTTGCCATCATCCTTGCAATCAGGGAGTATATATGGACATATGCAGCTCTGGAGTTTGCGCTTGTCGCCCTCATCGTCCACCTGTTCTACAGCATACTTCATCTGATGGCCATTTACGCGATATTGCTTGCTGCGGTGTTGGGTTTCGGATTAGCGATGAGCCTCAACTCATTGTACATGAAATATTTCACCTGGAGGGTTCAAGTTGCACAATCTCAAAGCTCTAGCCCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

414

Amino Acids

45.46

Weight (kDa)

7.55

Isoelectric Point (pI)

62.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 203 - 249 1.6e-12 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 708
AccII CGCG 1 cut(s) 1124
AciI CCGC 2 cut(s) 121, 1139
AclWI GGATC 1 cut(s) 486
AcoI YGGCCR 2 cut(s) 122, 1113
AcsI RAATTY 3 cut(s) 364, 585, 763
AcuI CTGAAG 3 cut(s) 95, 122, 612
AfaI GTAC 2 cut(s) 337, 1181
AfiI CCNNNNNNNGG 3 cut(s) 244, 307, 708
AgsI TTSAA 3 cut(s) 259, 943, 1208
AjnI CCWGG 4 cut(s) 102, 466, 860, 1196
Alw21I GWGCWC 4 cut(s) 325, 420, 676, 797
Alw26I GTCTC 3 cut(s) 25, 204, 995
Alw44I GTGCAC 1 cut(s) 416
AlwI GGATC 1 cut(s) 486
AlwNI CAGNNNCTG 1 cut(s) 928
AoxI GGCC 4 cut(s) 122, 152, 235, 1113
ApaLI GTGCAC 1 cut(s) 416
ApeKI GCWGC 7 cut(s) 8, 32, 219, 820, 922, 1049, 1136
ApoI RAATTY 3 cut(s) 364, 585, 763
Asp700I GAANNNNTTC 2 cut(s) 585, 1190
AspLEI GCGC 2 cut(s) 701, 1066
AspS9I GGNCC 3 cut(s) 235, 310, 453
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 296, 1186
AvaII GGWCC 2 cut(s) 310, 453
AxyI CCTNAGG 1 cut(s) 54
BaeGI GKGCMC 1 cut(s) 420
BalI TGGCCA 1 cut(s) 1115
BanII GRGCYC 1 cut(s) 325
BarI GAAGNNNNNNTAC 2 cut(s) 765, 797
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 4 cut(s) 325, 420, 676, 797
BbvI GCAGC 6 cut(s) 19, 231, 832, 934, 1061, 1123
BccI CCATC 3 cut(s) 275, 1019, 1105
BceAI ACGGC 1 cut(s) 159
BciT130I CCWGG 4 cut(s) 104, 468, 862, 1198
BclI TGATCA 1 cut(s) 544
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 3 cut(s) 25, 204, 995
BfaI CTAG 3 cut(s) 537, 948, 1230
BfmI CTRYAG 4 cut(s) 33, 194, 378, 1093
BisI GCNGC 8 cut(s) 9, 33, 122, 220, 821, 923, 1050, 1137
BlsI GCNGC 8 cut(s) 10, 34, 123, 221, 822, 924, 1051, 1138
Bme1390I CCNGG 5 cut(s) 104, 245, 468, 862, 1198
Bme18I GGWCC 2 cut(s) 310, 453
BmgT120I GGNCC 3 cut(s) 235, 310, 453
BmiI GGNNCC 5 cut(s) 108, 208, 236, 312, 455
BmrFI CCNGG 5 cut(s) 104, 245, 468, 862, 1198
BmrI ACTGGG 2 cut(s) 123, 309
BmsI GCATC 2 cut(s) 850, 1007
BmuI ACTGGG 2 cut(s) 123, 309
BpiI GAAGAC 1 cut(s) 83
BplI GAGNNNNNCTC 4 cut(s) 278, 310, 784, 816
BpmI CTGGAG 3 cut(s) 540, 1076, 1219
BpuEI CTTGAG 3 cut(s) 186, 510, 938
BpuMI CCSGG 1 cut(s) 245
BsaBI GATNNNNATC 1 cut(s) 496
BsaI GGTCTC 1 cut(s) 995
BsaJI CCNNGG 1 cut(s) 244
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 307, 708
Bse1I ACTGG 4 cut(s) 118, 315, 557, 563
Bse21I CCTNAGG 1 cut(s) 54
Bse3DI GCAATG 1 cut(s) 776
Bse8I GATNNNNATC 1 cut(s) 496
BseBI CCWGG 4 cut(s) 104, 468, 862, 1198
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 4 cut(s) 408, 841, 998, 1014
BseJI GATNNNNATC 1 cut(s) 496
BseLI CCNNNNNNNGG 3 cut(s) 244, 307, 708
BseMI GCAATG 1 cut(s) 776
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 4 cut(s) 118, 315, 557, 563
BseRI GAGGAG 1 cut(s) 42
BseSI GKGCMC 1 cut(s) 420
BseXI GCAGC 6 cut(s) 19, 231, 832, 934, 1061, 1123
BseYI CCCAGC 1 cut(s) 389
BsgI GTGCAG 2 cut(s) 628, 809
Bsh1236I CGCG 1 cut(s) 1124
BshFI GGCC 4 cut(s) 124, 154, 237, 1115
BsiHKAI GWGCWC 4 cut(s) 325, 420, 676, 797
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 2 cut(s) 355, 439
BslI CCNNNNNNNGG 3 cut(s) 244, 307, 708
BsmAI GTCTC 3 cut(s) 25, 204, 995
BsmFI GGGAC 2 cut(s) 355, 439
BsnI GGCC 4 cut(s) 124, 154, 237, 1115
Bso31I GGTCTC 1 cut(s) 995
Bsp1286I GDGCHC 4 cut(s) 325, 420, 676, 797
Bsp1407I TGTACA 1 cut(s) 1179
Bsp143I GATC 2 cut(s) 491, 544
BspACI CCGC 2 cut(s) 121, 1139
BspANI GGCC 4 cut(s) 124, 154, 237, 1115
BspCNI CTCAG 1 cut(s) 67
BspFNI CGCG 1 cut(s) 1124
BspLI GGNNCC 5 cut(s) 108, 208, 236, 312, 455
BspMAI CTGCAG 2 cut(s) 37, 198
BspPI GGATC 1 cut(s) 486
BspTNI GGTCTC 1 cut(s) 995
BsrDI GCAATG 1 cut(s) 776
BsrGI TGTACA 1 cut(s) 1179
BsrI ACTGG 4 cut(s) 118, 315, 557, 563
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 2 cut(s) 491, 544
Bst2UI CCWGG 4 cut(s) 104, 468, 862, 1198
Bst4CI ACNGT 3 cut(s) 82, 445, 972
Bst6I CTCTTC 2 cut(s) 348, 687
BstAPI GCANNNNNTGC 2 cut(s) 900, 974
BstAUI TGTACA 1 cut(s) 1179
BstC8I GCNNGC 3 cut(s) 6, 224, 1134
BstDEI CTNAG 2 cut(s) 54, 677
BstF5I GGATG 4 cut(s) 408, 841, 998, 1014
BstFNI CGCG 1 cut(s) 1124
BstHHI GCGC 2 cut(s) 701, 1066
BstKTI GATC 2 cut(s) 494, 547
BstMAI GTCTC 3 cut(s) 25, 204, 995
BstMBI GATC 2 cut(s) 491, 544
BstMWI GCNNNNNNNGC 5 cut(s) 787, 900, 974, 1070, 1121
BstNI CCWGG 4 cut(s) 104, 468, 862, 1198
BstSCI CCNGG 5 cut(s) 102, 243, 466, 860, 1196
BstSFI CTRYAG 4 cut(s) 33, 194, 378, 1093
BstSLI GKGCMC 1 cut(s) 420
BstUI CGCG 1 cut(s) 1124
BstV1I GCAGC 6 cut(s) 19, 231, 832, 934, 1061, 1123
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 1 cut(s) 491
BstYI RGATCY 1 cut(s) 491
Bsu36I CCTNAGG 1 cut(s) 54
BsuRI GGCC 4 cut(s) 124, 154, 237, 1115
BtgZI GCGATG 1 cut(s) 1175
BtsCI GGATG 4 cut(s) 408, 841, 998, 1014
BtsI GCAGTG 2 cut(s) 609, 795
BtsIMutI CAGTG 3 cut(s) 570, 609, 795
Cac8I GCNNGC 3 cut(s) 6, 224, 1134
CaiI CAGNNNCTG 1 cut(s) 928
CfoI GCGC 2 cut(s) 701, 1066
Cfr13I GGNCC 3 cut(s) 235, 310, 453
Csp6I GTAC 2 cut(s) 336, 1180
CviAII CATG 3 cut(s) 463, 689, 1183
CviQI GTAC 2 cut(s) 336, 1180
DdeI CTNAG 2 cut(s) 54, 677
DpnI GATC 2 cut(s) 493, 546
DpnII GATC 2 cut(s) 491, 544
EaeI YGGCCR 2 cut(s) 122, 1113
Eam1104I CTCTTC 2 cut(s) 348, 687
EarI CTCTTC 2 cut(s) 348, 687
Ecl136II GAGCTC 1 cut(s) 323
Eco147I AGGCCT 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 995
Eco32I GATATC 1 cut(s) 360
Eco47I GGWCC 2 cut(s) 310, 453
Eco53kI GAGCTC 1 cut(s) 323
Eco57I CTGAAG 3 cut(s) 95, 122, 612
Eco81I CCTNAGG 1 cut(s) 54
EcoICRI GAGCTC 1 cut(s) 323
EcoO109I RGGNCCY 2 cut(s) 235, 453
EcoRII CCWGG 4 cut(s) 102, 466, 860, 1196
EcoRV GATATC 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 325
FaeI CATG 3 cut(s) 466, 692, 1186
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 2 cut(s) 355, 439
FatI CATG 3 cut(s) 462, 688, 1182
FauNDI CATATG 1 cut(s) 1045
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 8 cut(s) 9, 33, 122, 220, 821, 923, 1050, 1137
FokI GGATG 4 cut(s) 415, 828, 985, 1001
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 8 cut(s) 9, 33, 122, 220, 821, 923, 1050, 1137
FspBI CTAG 3 cut(s) 537, 948, 1230
GlaI GCGC 2 cut(s) 700, 1065
GluI GCNGC 8 cut(s) 9, 33, 122, 220, 821, 923, 1050, 1137
GsaI CCCAGC 1 cut(s) 393
GsuI CTGGAG 3 cut(s) 540, 1076, 1219
HaeIII GGCC 4 cut(s) 124, 154, 237, 1115
HapII CCGG 1 cut(s) 244
HhaI GCGC 2 cut(s) 701, 1066
Hin1II CATG 3 cut(s) 466, 692, 1186
Hin6I GCGC 2 cut(s) 699, 1064
HinP1I GCGC 2 cut(s) 699, 1064
HindIII AAGCTT 1 cut(s) 253
HinfI GANTC 2 cut(s) 523, 931
HpaII CCGG 1 cut(s) 244
HphI GGTGA 2 cut(s) 296, 1186
Hpy166II GTNNAC 4 cut(s) 191, 418, 815, 1084
Hpy188I TCNGA 6 cut(s) 75, 141, 310, 762, 1110, 1154
Hpy188III TCNNGA 9 cut(s) 259, 326, 590, 620, 752, 845, 886, 935, 1055
Hpy8I GTNNAC 4 cut(s) 191, 418, 815, 1084
HpyAV CCTTC 2 cut(s) 57, 144
HpyCH4III ACNGT 3 cut(s) 82, 445, 972
HpyCH4IV ACGT 2 cut(s) 40, 472
HpyF10VI GCNNNNNNNGC 5 cut(s) 787, 900, 974, 1070, 1121
HpyF3I CTNAG 2 cut(s) 54, 677
HpySE526I ACGT 2 cut(s) 40, 472
Hsp92II CATG 3 cut(s) 466, 692, 1186
HspAI GCGC 2 cut(s) 699, 1064
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 2 cut(s) 491, 544
LmnI GCTCC 3 cut(s) 29, 299, 328
Lsp1109I GCAGC 6 cut(s) 19, 231, 832, 934, 1061, 1123
LweI GCATC 2 cut(s) 850, 1007
MaeI CTAG 3 cut(s) 537, 948, 1230
MaeII ACGT 2 cut(s) 40, 472
MaeIII GTNAC 2 cut(s) 772, 935
MalI GATC 2 cut(s) 493, 546
MboI GATC 2 cut(s) 491, 544
MboII GAAGA 9 cut(s) 88, 101, 335, 593, 605, 608, 704, 901, 904
MflI RGATCY 1 cut(s) 491
MhlI GDGCHC 4 cut(s) 325, 420, 676, 797
MlsI TGGCCA 1 cut(s) 1115
MluCI AATT 5 cut(s) 304, 364, 585, 763, 960
MluNI TGGCCA 1 cut(s) 1115
MlyI GAGTC 1 cut(s) 517
MmeI TCCRAC 1 cut(s) 48
Mox20I TGGCCA 1 cut(s) 1115
MroXI GAANNNNTTC 2 cut(s) 585, 1190
MscI TGGCCA 1 cut(s) 1115
MseI TTAA 4 cut(s) 213, 251, 303, 705
Msp20I TGGCCA 1 cut(s) 1115
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 5 cut(s) 104, 245, 468, 862, 1198
MvaI CCWGG 4 cut(s) 104, 468, 862, 1198
MvnI CGCG 1 cut(s) 1124
MwoI GCNNNNNNNGC 5 cut(s) 787, 900, 974, 1070, 1121
NciI CCSGG 1 cut(s) 245
NdeI CATATG 1 cut(s) 1045
NdeII GATC 2 cut(s) 491, 544
NlaIII CATG 3 cut(s) 466, 692, 1186
NlaIV GGNNCC 5 cut(s) 108, 208, 236, 312, 455
NmuCI GTSAC 1 cut(s) 935
PceI AGGCCT 1 cut(s) 154
PcsI WCGNNNNNNNCGW 1 cut(s) 1077
PdmI GAANNNNTTC 2 cut(s) 585, 1190
PfeI GAWTC 1 cut(s) 931
PflMI CCANNNNNTGG 1 cut(s) 708
PkrI GCNGC 8 cut(s) 10, 34, 123, 221, 822, 924, 1051, 1138
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
PpuMI RGGWCCY 1 cut(s) 453
Psp124BI GAGCTC 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 453
Psp6I CCWGG 4 cut(s) 102, 466, 860, 1196
PspFI CCCAGC 1 cut(s) 389
PspGI CCWGG 4 cut(s) 102, 466, 860, 1196
PspN4I GGNNCC 5 cut(s) 108, 208, 236, 312, 455
PspPI GGNCC 3 cut(s) 235, 310, 453
PspPPI RGGWCCY 1 cut(s) 453
PstI CTGCAG 2 cut(s) 37, 198
PstNI CAGNNNCTG 1 cut(s) 928
PsuI RGATCY 1 cut(s) 491
RsaI GTAC 2 cut(s) 337, 1181
RsaNI GTAC 2 cut(s) 336, 1180
SacI GAGCTC 1 cut(s) 325
SaqAI TTAA 4 cut(s) 213, 251, 303, 705
SatI GCNGC 8 cut(s) 9, 33, 122, 220, 821, 923, 1050, 1137
Sau3AI GATC 2 cut(s) 491, 544
Sau96I GGNCC 3 cut(s) 235, 310, 453
SchI GAGTC 1 cut(s) 517
ScrFI CCNGG 5 cut(s) 104, 245, 468, 862, 1198
SduI GDGCHC 4 cut(s) 325, 420, 676, 797
SfaNI GCATC 2 cut(s) 850, 1007
SfcI CTRYAG 4 cut(s) 33, 194, 378, 1093
SinI GGWCC 2 cut(s) 310, 453
SmlI CTYRAG 3 cut(s) 201, 525, 917
SmoI CTYRAG 3 cut(s) 201, 525, 917
Sse9I AATT 5 cut(s) 304, 364, 585, 763, 960
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 2 cut(s) 121, 1139
SspI AATATT 1 cut(s) 1190
SspMI CTAG 3 cut(s) 537, 948, 1230
SstI GAGCTC 1 cut(s) 325
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 5 cut(s) 102, 243, 466, 860, 1196
TaaI ACNGT 3 cut(s) 82, 445, 972
TaiI ACGT 2 cut(s) 43, 475
TaqI TCGA 2 cut(s) 449, 511
TasI AATT 5 cut(s) 304, 364, 585, 763, 960
TatI WGTACW 1 cut(s) 1179
TauI GCSGC 1 cut(s) 124
TfiI GAWTC 1 cut(s) 931
Tru1I TTAA 4 cut(s) 213, 251, 303, 705
Tru9I TTAA 4 cut(s) 213, 251, 303, 705
TscAI CASTG 3 cut(s) 570, 609, 802
TseFI GTSAC 1 cut(s) 935
TseI GCWGC 7 cut(s) 8, 32, 219, 820, 922, 1049, 1136
Tsp45I GTSAC 1 cut(s) 935
TspDTI ATGAA 7 cut(s) 102, 426, 594, 648, 705, 1094, 1199
TspGWI ACGGA 2 cut(s) 42, 484
TspRI CASTG 3 cut(s) 570, 609, 802
Van91I CCANNNNNTGG 1 cut(s) 708
VneI GTGCAC 1 cut(s) 416
VpaK11BI GGWCC 2 cut(s) 310, 453
XapI RAATTY 3 cut(s) 364, 585, 763
XmnI GAANNNNTTC 2 cut(s) 585, 1190
XspI CTAG 3 cut(s) 537, 948, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.