pycom13g15100

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005136
Physical Location & Seq
Reverse (-)
10977759 .. 10980389
2631 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g15100.1

Sequence Viewer

Length: 945 bp
ATGGCCAATTCCGTATCGCCATTTTCTTGGAAAAGATGTACCTCTCTTCCTGTTACGCCGGCGTCAAATTTGTCCCCCTCGGTTGCTACACCTATCTCCGCAAGGATGTATAGTGAAGCGCACAAGCAACATAAAGAAACTACAACAGTTTCGAGGTCCCTTTCTATGCCTGGACGAAATAAAGTCATTGTAAGATCCGTATCTTTTGCAACTAGAAATGGGCAGACTCAAACGGATCCTAGTGATGATCAAATAACTCCAGTTCCGGTGGAGGCCAATGATGAAGAAATTCCTGAAGAAGAAGCAGTGTGCAGGATATGTCTTGATGTATGTGATGAACAAAATACGCTCAAAATGGAATGCTTTTGCAAAGGTGCTCTCAGACTTCTACATGAAGAGTGCGCCATTAAGTGGTTTAGCACAAAAGGAAACAAGAAATGTGATGTATGTGGTCAAGAGGTTCAGAATTTACCTGTAACATTGCTTCGGGTTCAGAGCACTGCTCAAAGGGGTAGCAGACAGCAGCACAACCAACAGGGTTTGCATCCAGAAACGACAAGTGCCTGGCAGGACTTTGTGGTACTGGTTTTAATTAGCACCATATGCTATTTCTTCTTCCTTGAGCAGCTACTGATTCGTGACTTGAAAAATCAAGCGATTGTAATTGCAGCACCATTTGCATTTACATTGGGCCTCTTGGCATCCATATTCGCCATCATCCTTGCAATCAGGGAGTATATATGGACATATGCAGCTCTTGAGTTTGCGCTTGTCGCTCTAATTGTCCACCTGTTCTACAGCACACTTCAGCTGACTGCCATTTACTCGGTATTGCTCGCTGCGGTATTGGGTTTCGGGATAGCGATGAGCCTCAACTCATTGTATATCCAATATTACACTTGGAGAGTTCAAGTTGCACAAACTCAAAGCTCTAGCCCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

315

Amino Acids

35.02

Weight (kDa)

6.59

Isoelectric Point (pI)

55.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 104 - 150 1.4e-12 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 411
AciI CCGC 2 cut(s) 99, 842
AclWI GGATC 3 cut(s) 189, 230, 243
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 67, 288, 466
AcuI CTGAAG 2 cut(s) 315, 791
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 2 cut(s) 40, 582
AfiI CCNNNNNNNGG 1 cut(s) 411
AgsI TTSAA 2 cut(s) 646, 911
AjnI CCWGG 2 cut(s) 169, 563
AluBI AGCT 4 cut(s) 628, 755, 811, 930
AluI AGCT 4 cut(s) 628, 755, 811, 930
Alw21I GWGCWC 2 cut(s) 379, 500
AlwI GGATC 3 cut(s) 189, 230, 243
AlwNI CAGNNNCTG 1 cut(s) 631
AoxI GGCC 3 cut(s) 3, 273, 691
ApeKI GCWGC 5 cut(s) 523, 625, 668, 752, 839
ApoI RAATTY 3 cut(s) 67, 288, 466
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 3 cut(s) 121, 404, 769
AspS9I GGNCC 2 cut(s) 156, 691
AvaII GGWCC 1 cut(s) 156
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 235
BarI GAAGNNNNNNTAC 2 cut(s) 468, 500
Bbv12I GWGCWC 2 cut(s) 379, 500
BbvI GCAGC 5 cut(s) 535, 637, 680, 764, 826
BccI CCATC 1 cut(s) 722
BciT130I CCWGG 2 cut(s) 171, 565
BclI TGATCA 1 cut(s) 247
BfaI CTAG 3 cut(s) 213, 240, 933
BfmI CTRYAG 1 cut(s) 796
BisI GCNGC 5 cut(s) 524, 626, 669, 753, 840
BlsI GCNGC 5 cut(s) 525, 627, 670, 754, 841
Bme1390I CCNGG 2 cut(s) 171, 565
Bme18I GGWCC 1 cut(s) 156
BmgT120I GGNCC 2 cut(s) 156, 691
BmiI GGNNCC 2 cut(s) 158, 237
BmrFI CCNGG 2 cut(s) 171, 565
BmsI GCATC 2 cut(s) 553, 710
BplI GAGNNNNNCTC 2 cut(s) 487, 519
BpmI CTGGAG 1 cut(s) 243
BpuEI CTTGAG 2 cut(s) 641, 779
BsaBI GATNNNNATC 1 cut(s) 199
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 78
BsaWI WCCGGW 1 cut(s) 265
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 2 cut(s) 260, 588
Bse3DI GCAATG 1 cut(s) 479
Bse8I GATNNNNATC 1 cut(s) 199
BseBI CCWGG 2 cut(s) 171, 565
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 4 cut(s) 111, 544, 701, 717
BseJI GATNNNNATC 1 cut(s) 199
BseLI CCNNNNNNNGG 1 cut(s) 411
BseMI GCAATG 1 cut(s) 479
BseMII CTCAG 1 cut(s) 394
BseNI ACTGG 2 cut(s) 260, 588
BseXI GCAGC 5 cut(s) 535, 637, 680, 764, 826
BsgI GTGCAG 1 cut(s) 331
BshFI GGCC 3 cut(s) 5, 275, 693
BsiHKAI GWGCWC 2 cut(s) 379, 500
BsiSI CCGG 2 cut(s) 59, 266
BslFI GGGAC 2 cut(s) 58, 142
BslI CCNNNNNNNGG 1 cut(s) 411
BsmFI GGGAC 2 cut(s) 58, 142
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 3 cut(s) 5, 275, 693
Bsp1286I GDGCHC 2 cut(s) 379, 500
Bsp143I GATC 3 cut(s) 194, 235, 247
BspACI CCGC 2 cut(s) 99, 842
BspANI GGCC 3 cut(s) 5, 275, 693
BspCNI CTCAG 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 158, 237
BspPI GGATC 3 cut(s) 189, 230, 243
BsrDI GCAATG 1 cut(s) 479
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 2 cut(s) 260, 588
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 3 cut(s) 194, 235, 247
BssNI GRCGYC 1 cut(s) 62
Bst2UI CCWGG 2 cut(s) 171, 565
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 2 cut(s) 51, 390
BstACI GRCGYC 1 cut(s) 62
BstAPI GCANNNNNTGC 2 cut(s) 603, 677
BstC8I GCNNGC 2 cut(s) 60, 837
BstDEI CTNAG 1 cut(s) 380
BstF5I GGATG 4 cut(s) 111, 544, 701, 717
BstHHI GCGC 3 cut(s) 121, 404, 769
BstKTI GATC 3 cut(s) 197, 238, 250
BstMBI GATC 3 cut(s) 194, 235, 247
BstMWI GCNNNNNNNGC 3 cut(s) 603, 677, 773
BstNI CCWGG 2 cut(s) 171, 565
BstSCI CCNGG 2 cut(s) 169, 563
BstSFI CTRYAG 1 cut(s) 796
BstV1I GCAGC 5 cut(s) 535, 637, 680, 764, 826
BstX2I RGATCY 2 cut(s) 194, 235
BstXI CCANNNNNNTGG 1 cut(s) 27
BstYI RGATCY 2 cut(s) 194, 235
BsuRI GGCC 3 cut(s) 5, 275, 693
BtgZI GCGATG 1 cut(s) 878
BtsCI GGATG 4 cut(s) 111, 544, 701, 717
BtsI GCAGTG 2 cut(s) 312, 498
BtsIMutI CAGTG 2 cut(s) 312, 498
Cac8I GCNNGC 2 cut(s) 60, 837
CaiI CAGNNNCTG 1 cut(s) 631
CfoI GCGC 3 cut(s) 121, 404, 769
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 2 cut(s) 156, 691
CseI GACGC 1 cut(s) 51
Csp6I GTAC 2 cut(s) 39, 581
CviAII CATG 1 cut(s) 392
CviJI RGCY 9 cut(s) 5, 275, 628, 693, 755, 811, 870, 930, 936
CviKI_1 RGCY 9 cut(s) 5, 275, 628, 693, 755, 811, 870, 930, 936
CviQI GTAC 2 cut(s) 39, 581
DdeI CTNAG 1 cut(s) 380
DpnI GATC 3 cut(s) 196, 237, 249
DpnII GATC 3 cut(s) 194, 235, 247
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 51, 390
EarI CTCTTC 2 cut(s) 51, 390
Eco47I GGWCC 1 cut(s) 156
Eco57I CTGAAG 2 cut(s) 315, 791
EcoO109I RGGNCCY 1 cut(s) 156
EcoRII CCWGG 2 cut(s) 169, 563
FaeI CATG 1 cut(s) 395
FaqI GGGAC 2 cut(s) 58, 142
FatI CATG 1 cut(s) 391
FauNDI CATATG 2 cut(s) 602, 748
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 5 cut(s) 524, 626, 669, 753, 840
FokI GGATG 4 cut(s) 118, 531, 688, 704
Fsp4HI GCNGC 5 cut(s) 524, 626, 669, 753, 840
FspBI CTAG 3 cut(s) 213, 240, 933
GlaI GCGC 3 cut(s) 120, 403, 768
GluI GCNGC 5 cut(s) 524, 626, 669, 753, 840
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 3 cut(s) 5, 275, 693
HapII CCGG 2 cut(s) 59, 266
HgaI GACGC 1 cut(s) 51
HhaI GCGC 3 cut(s) 121, 404, 769
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 1 cut(s) 395
Hin6I GCGC 3 cut(s) 119, 402, 767
HinP1I GCGC 3 cut(s) 119, 402, 767
HinfI GANTC 2 cut(s) 226, 634
HpaII CCGG 2 cut(s) 59, 266
Hpy166II GTNNAC 1 cut(s) 787
Hpy188I TCNGA 3 cut(s) 383, 465, 495
Hpy188III TCNNGA 7 cut(s) 293, 323, 455, 548, 638, 758, 856
Hpy8I GTNNAC 1 cut(s) 787
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4V TGCA 9 cut(s) 209, 312, 369, 544, 668, 680, 725, 752, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 603, 677, 773
HpyF3I CTNAG 1 cut(s) 380
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 1 cut(s) 395
HspAI GCGC 3 cut(s) 119, 402, 767
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 3 cut(s) 194, 235, 247
Lsp1109I GCAGC 5 cut(s) 535, 637, 680, 764, 826
LweI GCATC 2 cut(s) 553, 710
MaeI CTAG 3 cut(s) 213, 240, 933
MaeIII GTNAC 3 cut(s) 52, 475, 638
MalI GATC 3 cut(s) 196, 237, 249
MboI GATC 3 cut(s) 194, 235, 247
MboII GAAGA 7 cut(s) 38, 296, 308, 311, 407, 604, 607
MflI RGATCY 2 cut(s) 194, 235
MhlI GDGCHC 2 cut(s) 379, 500
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 7, 67, 288, 466, 591, 663, 780
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 220
MnlI CCTC 7 cut(s) 52, 88, 147, 265, 451, 704, 881
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 58
MroNI GCCGGC 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 288
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 408, 590
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 811
MspI CCGG 2 cut(s) 59, 266
MspR9I CCNGG 2 cut(s) 171, 565
Mva1269I GAATGC 1 cut(s) 365
MvaI CCWGG 2 cut(s) 171, 565
MwoI GCNNNNNNNGC 3 cut(s) 603, 677, 773
NaeI GCCGGC 1 cut(s) 60
NdeI CATATG 2 cut(s) 602, 748
NdeII GATC 3 cut(s) 194, 235, 247
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 1 cut(s) 395
NlaIV GGNNCC 2 cut(s) 158, 237
NmuCI GTSAC 1 cut(s) 638
PctI GAATGC 1 cut(s) 365
PdiI GCCGGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 1 cut(s) 634
PflMI CCANNNNNTGG 1 cut(s) 411
PkrI GCNGC 5 cut(s) 525, 627, 670, 754, 841
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 169, 563
PspGI CCWGG 2 cut(s) 169, 563
PspN4I GGNNCC 2 cut(s) 158, 237
PspPI GGNCC 2 cut(s) 156, 691
PspPPI RGGWCCY 1 cut(s) 156
PstNI CAGNNNCTG 1 cut(s) 631
PsuI RGATCY 2 cut(s) 194, 235
PvuII CAGCTG 1 cut(s) 811
RsaI GTAC 2 cut(s) 40, 582
RsaNI GTAC 2 cut(s) 39, 581
SaqAI TTAA 2 cut(s) 408, 590
SatI GCNGC 5 cut(s) 524, 626, 669, 753, 840
Sau3AI GATC 3 cut(s) 194, 235, 247
Sau96I GGNCC 2 cut(s) 156, 691
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 2 cut(s) 171, 565
SduI GDGCHC 2 cut(s) 379, 500
SfaNI GCATC 2 cut(s) 553, 710
SfcI CTRYAG 1 cut(s) 796
SgrAI CRCCGGYG 1 cut(s) 58
SinI GGWCC 1 cut(s) 156
SmlI CTYRAG 2 cut(s) 620, 758
SmoI CTYRAG 2 cut(s) 620, 758
Sse9I AATT 7 cut(s) 7, 67, 288, 466, 591, 663, 780
SsiI CCGC 2 cut(s) 99, 842
SspI AATATT 1 cut(s) 893
SspMI CTAG 3 cut(s) 213, 240, 933
StyD4I CCNGG 2 cut(s) 169, 563
TaaI ACNGT 1 cut(s) 148
TaqI TCGA 1 cut(s) 152
TasI AATT 7 cut(s) 7, 67, 288, 466, 591, 663, 780
TfiI GAWTC 1 cut(s) 634
Tru1I TTAA 2 cut(s) 408, 590
Tru9I TTAA 2 cut(s) 408, 590
TscAI CASTG 2 cut(s) 312, 505
TseFI GTSAC 1 cut(s) 638
TseI GCWGC 5 cut(s) 523, 625, 668, 752, 839
Tsp45I GTSAC 1 cut(s) 638
TspDTI ATGAA 3 cut(s) 297, 351, 408
TspGWI ACGGA 2 cut(s) 187, 248
TspRI CASTG 2 cut(s) 312, 505
Van91I CCANNNNNTGG 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 156
XapI RAATTY 3 cut(s) 67, 288, 466
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 3 cut(s) 213, 240, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.