Rh4CG241900

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005136
Physical Location & Seq
Forward (+)
48857941 .. 48861950
4010 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG241900.1

Sequence Viewer

Length: 1284 bp
ATGCAAGCAACTGAAGCAGTGAGTAATGGGGTTACAGAAGCTCCTCCTCCTCAGGCTGGTGCTCAAAATCTCAAGTCCATACAGGAAGAACCCACAGAACCAGGGCATAGTGGAAGACGCCCCGATATATCACTTCAAATACCCCCAAGACATGCGGCGTTTGGCAGTAGCCGGAGCGGCAAGGGTTTACTCCAGTCTCAAGGTTCCTCTAAGGGTAGTCCACTTTCAGGAGGGTTATTCCGGGCTTTAAGCTTCAAGAAGAAAGGTATACTGCCGGATGGTGAGAAAAGTTTCCTCCTTAATTCAGACTCCAGTACAGCTCCGGGAAGTCCAGTAGTGGCCAATAATGCACCAGCATTTTCTTGGACAAAGTGTGCCTCTCTTCCTGTTACACCAGCATCGAATTTGTCACCATCCATTTCTACACCAGTTTCTGCAAGGATGTATAATGAACGGCACAAGCCACATAAAGAAACTGTTAAGGCTACAGTTTCAAGGTCCCTGTCTGTGCCTGGACAAAATATAGTTATTGTTAGATCCGTATCTTTTGCTGCTCGAAATGAGAACATCCAGACAGATCCTAGTGATGATCAAATAACTCCGGTTCCAGTTGAGCCTGATGATGAAGAAATTCCTGAAGAAGAAGCAGTATGCAGGATTTGTCTTGATGAATGTGAAGAACAAAATACACTCAAAATGGAATGCAGTTGCAAAGGTGCTCTCAGACTTGTACATGAACATTGTGCAATTAAGTGGTTTAGCACAAGAGGAAACAGGACATGCGAGGTATGTGGGCAGGAGGTTCAGAATTTACCTGTAACCTTGCTTCGGGTGCAGAGCTCTGTTCAAAGGGCCAGCAGACAGAACCAGAACCAACAGAGTTTGAATCCAGAAATGATAAGTGCATGGCAGGACTTCGTGATACTGGTATTAATTAGCACAATATGCTACTTCTTCTTCCTTGAGCAACTACTGATTCGTGACTTGAAAACTCAAGCGATTGTAGTTGCAGCACCATTTGCATTTACATTGGGTCTCTTGGCATCCATATTTGCCATTGTCCTTGCAATCAAGGAGTATATATGGACATATGCGGCTCTCGAGTTTGGACTTGTAGCGCTCACCGTACACCTTTTATATTCTACATTTCATCTGCCTCCTATTTATTCAATATTGCTTGCATCAGTAGTGGGTTTTGGGATATCAATGACCCTCAACTCCTTGTATATGCAATACTTCACTTGGAGAGTTCAAGTTTCTCAAAACAACTCTAGCCCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

427

Amino Acids

46.63

Weight (kDa)

6.47

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 218 - 264 1.2e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 177
AccI GTMKAC 1 cut(s) 268
AciI CCGC 3 cut(s) 155, 177, 1094
AclWI GGATC 2 cut(s) 531, 572
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 3 cut(s) 403, 630, 808
AcuI CTGAAG 2 cut(s) 33, 657
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 3 cut(s) 316, 732, 1128
AfeI AGCGCT 1 cut(s) 1119
AfiI CCNNNNNNNGG 3 cut(s) 56, 227, 828
AgsI TTSAA 8 cut(s) 137, 256, 495, 848, 886, 988, 1170, 1253
AjnI CCWGG 2 cut(s) 100, 511
AluBI AGCT 4 cut(s) 41, 252, 320, 840
AluI AGCT 4 cut(s) 41, 252, 320, 840
Alw21I GWGCWC 3 cut(s) 64, 721, 842
Alw26I GTCTC 2 cut(s) 201, 1040
AlwI GGATC 2 cut(s) 531, 572
AlwNI CAGNNNCTG 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 1100
Aor51HI AGCGCT 1 cut(s) 1119
AoxI GGCC 2 cut(s) 339, 852
ApeKI GCWGC 2 cut(s) 551, 1010
ApoI RAATTY 3 cut(s) 403, 630, 808
AseI ATTAAT 1 cut(s) 932
Asp700I GAANNNNTTC 2 cut(s) 290, 630
AspLEI GCGC 1 cut(s) 1120
AspS9I GGNCC 2 cut(s) 498, 852
AsuC2I CCSGG 2 cut(s) 242, 324
AsuHPI GGTGA 3 cut(s) 293, 402, 1114
AvaI CYCGRG 1 cut(s) 1100
AvaII GGWCC 1 cut(s) 498
AxyI CCTNAGG 1 cut(s) 51
BalI TGGCCA 1 cut(s) 341
BanII GRGCYC 1 cut(s) 842
BarI GAAGNNNNNNTAC 2 cut(s) 810, 842
BbsI GAAGAC 1 cut(s) 121
Bbv12I GWGCWC 3 cut(s) 64, 721, 842
BbvI GCAGC 2 cut(s) 538, 1022
BccI CCATC 2 cut(s) 272, 421
BceAI ACGGC 1 cut(s) 470
BciT130I CCWGG 2 cut(s) 102, 513
BclI TGATCA 1 cut(s) 589
BcnI CCSGG 2 cut(s) 242, 324
BcoDI GTCTC 2 cut(s) 201, 1040
BfaI CTAG 2 cut(s) 582, 1272
BfmI CTRYAG 1 cut(s) 486
BfoI RGCGCY 1 cut(s) 1121
BglI GCCNNNNNGGC 1 cut(s) 177
BisI GCNGC 5 cut(s) 156, 178, 552, 1011, 1095
BlsI GCNGC 5 cut(s) 157, 179, 553, 1012, 1096
Bme1390I CCNGG 4 cut(s) 102, 242, 324, 513
Bme18I GGWCC 1 cut(s) 498
BmeT110I CYCGRG 1 cut(s) 1100
BmgT120I GGNCC 2 cut(s) 498, 852
BmiI GGNNCC 3 cut(s) 205, 500, 606
BmrFI CCNGG 4 cut(s) 102, 242, 324, 513
BmsI GCATC 3 cut(s) 407, 1052, 1190
BpiI GAAGAC 1 cut(s) 121
BpmI CTGGAG 2 cut(s) 176, 295
BpuEI CTTGAG 4 cut(s) 56, 183, 978, 983
BpuMI CCSGG 2 cut(s) 242, 324
BsaBI GATNNNNATC 1 cut(s) 541
BsaHI GRCGYC 1 cut(s) 118
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 1 cut(s) 101
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 2 cut(s) 25, 55
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 227, 828
Bse1I ACTGG 6 cut(s) 193, 312, 332, 428, 608, 930
Bse21I CCTNAGG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 541
BseBI CCWGG 2 cut(s) 102, 513
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 5 cut(s) 283, 413, 447, 567, 1043
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 3 cut(s) 56, 227, 828
BseMII CTCAG 2 cut(s) 65, 736
BseNI ACTGG 6 cut(s) 193, 312, 332, 428, 608, 930
BseRI GAGGAG 3 cut(s) 33, 36, 39
BseXI GCAGC 2 cut(s) 538, 1022
BseYI CCCAGC 1 cut(s) 1276
BsgI GTGCAG 1 cut(s) 854
BshFI GGCC 2 cut(s) 341, 854
BsiHKAI GWGCWC 3 cut(s) 64, 721, 842
BsiHKCI CYCGRG 1 cut(s) 1100
BsiSI CCGG 5 cut(s) 172, 241, 275, 323, 602
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 3 cut(s) 56, 227, 828
BsmAI GTCTC 2 cut(s) 201, 1040
BsmFI GGGAC 1 cut(s) 484
BsmI GAATGC 1 cut(s) 707
BsnI GGCC 2 cut(s) 341, 854
Bso31I GGTCTC 1 cut(s) 1040
BsoBI CYCGRG 1 cut(s) 1100
Bsp1286I GDGCHC 3 cut(s) 64, 721, 842
Bsp1407I TGTACA 1 cut(s) 730
Bsp143I GATC 3 cut(s) 536, 577, 589
BspACI CCGC 3 cut(s) 155, 177, 1094
BspANI GGCC 2 cut(s) 341, 854
BspCNI CTCAG 2 cut(s) 64, 735
BspLI GGNNCC 3 cut(s) 205, 500, 606
BspPI GGATC 2 cut(s) 531, 572
BspTNI GGTCTC 1 cut(s) 1040
BsrBI CCGCTC 1 cut(s) 177
BsrGI TGTACA 1 cut(s) 730
BsrI ACTGG 6 cut(s) 193, 312, 332, 428, 608, 930
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 3 cut(s) 536, 577, 589
BssNAI GTATAC 1 cut(s) 269
BssNI GRCGYC 1 cut(s) 118
Bst1107I GTATAC 1 cut(s) 269
Bst2UI CCWGG 2 cut(s) 102, 513
Bst4CI ACNGT 3 cut(s) 478, 490, 1126
Bst6I CTCTTC 1 cut(s) 387
BstACI GRCGYC 1 cut(s) 118
BstAPI GCANNNNNTGC 2 cut(s) 945, 1019
BstAUI TGTACA 1 cut(s) 730
BstC8I GCNNGC 3 cut(s) 6, 856, 1179
BstDEI CTNAG 3 cut(s) 51, 210, 722
BstF5I GGATG 5 cut(s) 283, 413, 447, 567, 1043
BstH2I RGCGCY 1 cut(s) 1121
BstHHI GCGC 1 cut(s) 1120
BstKTI GATC 3 cut(s) 539, 580, 592
BstMAI GTCTC 2 cut(s) 201, 1040
BstMBI GATC 3 cut(s) 536, 577, 589
BstMWI GCNNNNNNNGC 6 cut(s) 14, 177, 347, 832, 945, 1019
BstNI CCWGG 2 cut(s) 102, 513
BstNSI RCATGY 2 cut(s) 155, 783
BstSCI CCNGG 4 cut(s) 100, 240, 322, 511
BstSFI CTRYAG 1 cut(s) 486
BstV1I GCAGC 2 cut(s) 538, 1022
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 2 cut(s) 536, 577
BstYI RGATCY 2 cut(s) 536, 577
BstZ17I GTATAC 1 cut(s) 269
Bsu36I CCTNAGG 1 cut(s) 51
BsuRI GGCC 2 cut(s) 341, 854
BtsCI GGATG 5 cut(s) 283, 413, 447, 567, 1043
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 3 cut(s) 6, 856, 1179
CaiI CAGNNNCTG 1 cut(s) 434
CfoI GCGC 1 cut(s) 1120
Cfr13I GGNCC 2 cut(s) 498, 852
CseI GACGC 1 cut(s) 126
Csp6I GTAC 3 cut(s) 315, 731, 1127
CviAII CATG 5 cut(s) 152, 734, 780, 906, 1281
CviQI GTAC 3 cut(s) 315, 731, 1127
DdeI CTNAG 3 cut(s) 51, 210, 722
DpnI GATC 3 cut(s) 538, 579, 591
DpnII GATC 3 cut(s) 536, 577, 589
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
Ecl136II GAGCTC 1 cut(s) 840
Eco24I GRGCYC 1 cut(s) 842
Eco31I GGTCTC 1 cut(s) 1040
Eco32I GATATC 1 cut(s) 1203
Eco47I GGWCC 1 cut(s) 498
Eco47III AGCGCT 1 cut(s) 1119
Eco53kI GAGCTC 1 cut(s) 840
Eco57I CTGAAG 2 cut(s) 33, 657
Eco81I CCTNAGG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 1100
EcoICRI GAGCTC 1 cut(s) 840
EcoO109I RGGNCCY 1 cut(s) 498
EcoRII CCWGG 2 cut(s) 100, 511
EcoRV GATATC 1 cut(s) 1203
EcoT38I GRGCYC 1 cut(s) 842
FaeI CATG 5 cut(s) 155, 737, 783, 909, 1284
FaqI GGGAC 1 cut(s) 484
FatI CATG 5 cut(s) 151, 733, 779, 905, 1280
FauNDI CATATG 1 cut(s) 1090
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 1 cut(s) 268
Fnu4HI GCNGC 5 cut(s) 156, 178, 552, 1011, 1095
FokI GGATG 5 cut(s) 290, 400, 454, 554, 1030
FriOI GRGCYC 1 cut(s) 842
Fsp4HI GCNGC 5 cut(s) 156, 178, 552, 1011, 1095
FspBI CTAG 2 cut(s) 582, 1272
GlaI GCGC 1 cut(s) 1119
GluI GCNGC 5 cut(s) 156, 178, 552, 1011, 1095
GsaI CCCAGC 1 cut(s) 1280
GsuI CTGGAG 2 cut(s) 176, 295
HaeII RGCGCY 1 cut(s) 1121
HaeIII GGCC 2 cut(s) 341, 854
HapII CCGG 5 cut(s) 172, 241, 275, 323, 602
HgaI GACGC 1 cut(s) 126
HhaI GCGC 1 cut(s) 1120
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 5 cut(s) 155, 737, 783, 909, 1284
Hin6I GCGC 1 cut(s) 1118
HinP1I GCGC 1 cut(s) 1118
HindIII AAGCTT 1 cut(s) 250
HinfI GANTC 3 cut(s) 308, 886, 976
HpaII CCGG 5 cut(s) 172, 241, 275, 323, 602
HphI GGTGA 3 cut(s) 293, 402, 1114
Hpy166II GTNNAC 4 cut(s) 188, 221, 269, 1129
Hpy188I TCNGA 3 cut(s) 307, 725, 807
Hpy188III TCNNGA 9 cut(s) 228, 256, 571, 635, 665, 890, 919, 980, 1100
Hpy8I GTNNAC 4 cut(s) 188, 221, 269, 1129
HpyCH4III ACNGT 3 cut(s) 478, 490, 1126
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 177, 347, 832, 945, 1019
HpyF3I CTNAG 3 cut(s) 51, 210, 722
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 5 cut(s) 155, 737, 783, 909, 1284
HspAI GCGC 1 cut(s) 1118
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 3 cut(s) 536, 577, 589
LmnI GCTCC 3 cut(s) 46, 174, 325
Lsp1109I GCAGC 2 cut(s) 538, 1022
LweI GCATC 3 cut(s) 407, 1052, 1190
MaeI CTAG 2 cut(s) 582, 1272
MaeIII GTNAC 5 cut(s) 31, 388, 408, 817, 980
MalI GATC 3 cut(s) 538, 579, 591
MbiI CCGCTC 1 cut(s) 177
MboI GATC 3 cut(s) 536, 577, 589
MflI RGATCY 2 cut(s) 536, 577
MhlI GDGCHC 3 cut(s) 64, 721, 842
MlsI TGGCCA 1 cut(s) 341
MluCI AATT 6 cut(s) 301, 403, 630, 747, 808, 933
MluNI TGGCCA 1 cut(s) 341
MlyI GAGTC 1 cut(s) 302
Mox20I TGGCCA 1 cut(s) 341
MroXI GAANNNNTTC 2 cut(s) 290, 630
MscI TGGCCA 1 cut(s) 341
MseI TTAA 5 cut(s) 248, 300, 480, 750, 932
Msp20I TGGCCA 1 cut(s) 341
MspI CCGG 5 cut(s) 172, 241, 275, 323, 602
MspR9I CCNGG 4 cut(s) 102, 242, 324, 513
Mva1269I GAATGC 1 cut(s) 707
MvaI CCWGG 2 cut(s) 102, 513
MwoI GCNNNNNNNGC 6 cut(s) 14, 177, 347, 832, 945, 1019
NciI CCSGG 2 cut(s) 242, 324
NdeI CATATG 1 cut(s) 1090
NdeII GATC 3 cut(s) 536, 577, 589
NlaIII CATG 5 cut(s) 155, 737, 783, 909, 1284
NlaIV GGNNCC 3 cut(s) 205, 500, 606
NmuCI GTSAC 2 cut(s) 408, 980
NspI RCATGY 2 cut(s) 155, 783
PaeR7I CTCGAG 1 cut(s) 1100
PctI GAATGC 1 cut(s) 707
PdmI GAANNNNTTC 2 cut(s) 290, 630
PfeI GAWTC 2 cut(s) 886, 976
PfoI TCCNGGA 1 cut(s) 322
PkrI GCNGC 5 cut(s) 157, 179, 553, 1012, 1096
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PpuMI RGGWCCY 1 cut(s) 498
PshBI ATTAAT 1 cut(s) 932
Psp124BI GAGCTC 1 cut(s) 842
Psp5II RGGWCCY 1 cut(s) 498
Psp6I CCWGG 2 cut(s) 100, 511
PspFI CCCAGC 1 cut(s) 1276
PspGI CCWGG 2 cut(s) 100, 511
PspN4I GGNNCC 3 cut(s) 205, 500, 606
PspPI GGNCC 2 cut(s) 498, 852
PspPPI RGGWCCY 1 cut(s) 498
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 2 cut(s) 536, 577
RsaI GTAC 3 cut(s) 316, 732, 1128
RsaNI GTAC 3 cut(s) 315, 731, 1127
SacI GAGCTC 1 cut(s) 842
SaqAI TTAA 5 cut(s) 248, 300, 480, 750, 932
SatI GCNGC 5 cut(s) 156, 178, 552, 1011, 1095
Sau3AI GATC 3 cut(s) 536, 577, 589
Sau96I GGNCC 2 cut(s) 498, 852
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 4 cut(s) 102, 242, 324, 513
SduI GDGCHC 3 cut(s) 64, 721, 842
SfaNI GCATC 3 cut(s) 407, 1052, 1190
SfcI CTRYAG 1 cut(s) 486
Sfr274I CTCGAG 1 cut(s) 1100
SinI GGWCC 1 cut(s) 498
SlaI CTCGAG 1 cut(s) 1100
SmlI CTYRAG 5 cut(s) 71, 198, 962, 993, 1100
SmoI CTYRAG 5 cut(s) 71, 198, 962, 993, 1100
Sse9I AATT 6 cut(s) 301, 403, 630, 747, 808, 933
SsiI CCGC 3 cut(s) 155, 177, 1094
SspI AATATT 1 cut(s) 1173
SspMI CTAG 2 cut(s) 582, 1272
SstI GAGCTC 1 cut(s) 842
StyD4I CCNGG 4 cut(s) 100, 240, 322, 511
TaaI ACNGT 3 cut(s) 478, 490, 1126
TaqI TCGA 3 cut(s) 401, 556, 1101
TasI AATT 6 cut(s) 301, 403, 630, 747, 808, 933
TatI WGTACW 2 cut(s) 314, 730
TauI GCSGC 3 cut(s) 158, 180, 1097
TfiI GAWTC 2 cut(s) 886, 976
Tru1I TTAA 5 cut(s) 248, 300, 480, 750, 932
Tru9I TTAA 5 cut(s) 248, 300, 480, 750, 932
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 2 cut(s) 408, 980
TseI GCWGC 2 cut(s) 551, 1010
Tsp45I GTSAC 2 cut(s) 408, 980
TspDTI ATGAA 5 cut(s) 465, 639, 684, 750, 1139
TspGWI ACGGA 1 cut(s) 529
TspRI CASTG 1 cut(s) 24
VpaK11BI GGWCC 1 cut(s) 498
VspI ATTAAT 1 cut(s) 932
XapI RAATTY 3 cut(s) 403, 630, 808
XceI RCATGY 2 cut(s) 155, 783
XcmI CCANNNNNNNNNTGG 1 cut(s) 360
XhoI CTCGAG 1 cut(s) 1100
XmiI GTMKAC 1 cut(s) 268
XmnI GAANNNNTTC 2 cut(s) 290, 630
XspI CTAG 2 cut(s) 582, 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.