MD13G1175100.v1.1

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005136
Physical Location & Seq
Reverse (-)
14451757 .. 14455742
3986 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1175100.v1.1.491

Sequence Viewer

Length: 1311 bp
ATGCAAGCAGCTCAATCAGTCTCCAATGGAGCTGTAGAATCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCCCCTCAGGGTGGTCTCCAAAATCTGAAGACAGTAAAGGAAGAAGGCACCGAACCAGAACCTCCCGGGAGGCGCCCGGATATATCACTTCAGATACCCCCAAGGCCTGCAGGTTTTGGCAGTAGCCGTAGCGGAAAGGGTTTACTACAGTCTCAAGGTTCCTCTAAGGGTAGCTCGCTATCAGGCGGCCTCTTCCGGGGTTTAAGCTTCAAGAAAAAAGGTAACCAACCGGATGGTGAGAAAAGCTCCCTCCTTAATTCAGGCCCCAGTAGAGCTCCTGAAAGTCCTACAATGGCCAATTCAGTATCGCCATTTTCTTGGAAAAGATGTACCTCTCTTCCTGTTACGCCGGCATCAAATTTGTCCCCCTCTGTTGCTACGCCTATATCCGCAAGGATGTATAATGAAGCGCACAAGCAACATAAAGAAACTACAACAGTTTCGAGATCCCTTTCTATGCCTGGACAAAATAAAGTCATTGTAAGATCCGTATCTTTTGCAACTAGAAATGGGCAGACTCAAACAGATCCTAGTGATGATCAAATAACTCCAGTTCCGGTGGAGGCCAATGATGAAGAAATTCCTGAAGAAGAAGCGGTGTGCAGGATATGTCTTGATGTATGTGATGAACAAAATACGCTCAAAATGGAATGCTTTTGCAAAGGTGCTCTCAGACTTCTACATGAAGAGTGCGCCATTAAGTGGTTTAGCACAAAAGGAAACAAGAAATGTGATGTATGTGGTCAAGAGGTTCAGAATTTACCTGTAACATTGCTTCGGGTGCAGAGCACTGCTCAAAGGGGTAGCAGACAGCAGCACAACCAACAGGGTTTGCATCCAGAAACGATAAGTGCCTGGCAGGACTTTGTGGTACTGGTTTTAATTAGCACCATATGCTATTTCTTCTTCCTTGAGCAGCTACTGATTCGTGACATGAAAAATCAAGCCATTGTAATTGCAGCACCATTTGCATTTACATTGAGCCTCTTGGCATCCATATTCGCCATCATCCTTGCAATCAGGGAGTATATATGGACATATGCAGCTCTTGAGTTTGCGCTTGTCGCCCTAATCGTCCACCTGTTCTACAGCACACTTCATCTGACTGCCATTTACTCGATATTGCTCGCTGCGGTATTGGGTTTCGGGATAGCGATGAGCCTCAACTCATTGTATATCCAATATTACACCTGGAGAGTTCAAGTCGCACAAACTCAAAGCTCTAGCCCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

437

Amino Acids

47.52

Weight (kDa)

8.27

Isoelectric Point (pI)

63.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 226 - 272 2.2e-12 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 176
AccB1I GGYRCC 2 cut(s) 122, 147
AccB7I CCANNNNNTGG 1 cut(s) 777
AciI CCGC 5 cut(s) 207, 261, 465, 671, 1208
AclWI GGATC 3 cut(s) 516, 555, 596
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 3 cut(s) 433, 654, 832
AcuI CTGAAG 3 cut(s) 122, 149, 681
AcyI GRCGYC 1 cut(s) 148
AfaI GTAC 2 cut(s) 406, 948
AfiI CCNNNNNNNGG 4 cut(s) 83, 86, 271, 777
AgsI TTSAA 2 cut(s) 286, 1277
AjnI CCWGG 3 cut(s) 535, 929, 1265
AluBI AGCT 9 cut(s) 11, 32, 249, 282, 321, 350, 994, 1121, 1296
AluI AGCT 9 cut(s) 11, 32, 249, 282, 321, 350, 994, 1121, 1296
Alw21I GWGCWC 3 cut(s) 352, 745, 866
Alw26I GTCTC 3 cut(s) 25, 95, 231
AlwI GGATC 3 cut(s) 516, 555, 596
AlwNI CAGNNNCTG 1 cut(s) 997
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 5 cut(s) 179, 262, 337, 369, 639
ApeKI GCWGC 6 cut(s) 8, 889, 991, 1034, 1118, 1205
ApoI RAATTY 3 cut(s) 433, 654, 832
Asp700I GAANNNNTTC 1 cut(s) 654
AspLEI GCGC 4 cut(s) 150, 487, 770, 1135
AspS9I GGNCC 1 cut(s) 338
AsuC2I CCSGG 4 cut(s) 141, 142, 152, 272
AsuHPI GGTGA 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 140
AxyI CCTNAGG 1 cut(s) 81
BalI TGGCCA 1 cut(s) 371
BanI GGYRCC 2 cut(s) 122, 147
BanII GRGCYC 1 cut(s) 352
BarI GAAGNNNNNNTAC 2 cut(s) 834, 866
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 3 cut(s) 352, 745, 866
BbvI GCAGC 6 cut(s) 20, 901, 1003, 1046, 1130, 1192
BccI CCATC 2 cut(s) 302, 1088
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 3 cut(s) 537, 931, 1267
BclI TGATCA 1 cut(s) 613
BcnI CCSGG 4 cut(s) 141, 142, 152, 272
BcoDI GTCTC 3 cut(s) 25, 95, 231
BfaI CTAG 3 cut(s) 579, 606, 1299
BfmI CTRYAG 4 cut(s) 33, 183, 221, 1162
BfoI RGCGCY 1 cut(s) 151
BfuAI ACCTGC 1 cut(s) 176
BisI GCNGC 7 cut(s) 9, 262, 890, 992, 1035, 1119, 1206
BlsI GCNGC 7 cut(s) 10, 263, 891, 993, 1036, 1120, 1207
Bme1390I CCNGG 7 cut(s) 141, 142, 152, 272, 537, 931, 1267
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 4 cut(s) 124, 149, 235, 340
BmrFI CCNGG 7 cut(s) 141, 142, 152, 272, 537, 931, 1267
BmrI ACTGGG 1 cut(s) 336
BmsI GCATC 3 cut(s) 437, 919, 1076
BmuI ACTGGG 1 cut(s) 336
BpiI GAAGAC 1 cut(s) 110
BplI GAGNNNNNCTC 4 cut(s) 305, 337, 853, 885
BpmI CTGGAG 2 cut(s) 609, 1288
BpuEI CTTGAG 3 cut(s) 213, 1007, 1145
BpuMI CCSGG 4 cut(s) 141, 142, 152, 272
BsaBI GATNNNNATC 1 cut(s) 565
BsaHI GRCGYC 1 cut(s) 148
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 140, 176, 271
BsaWI WCCGGW 2 cut(s) 304, 631
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 86, 271, 777
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 3 cut(s) 342, 626, 954
Bse21I CCTNAGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 845
Bse8I GATNNNNATC 1 cut(s) 565
BseBI CCWGG 3 cut(s) 537, 931, 1267
BseDI CCNNGG 3 cut(s) 140, 176, 271
BseGI GGATG 5 cut(s) 313, 477, 910, 1067, 1083
BseJI GATNNNNATC 1 cut(s) 565
BseLI CCNNNNNNNGG 4 cut(s) 83, 86, 271, 777
BseMI GCAATG 1 cut(s) 845
BseMII CTCAG 2 cut(s) 95, 760
BseNI ACTGG 3 cut(s) 342, 626, 954
BseXI GCAGC 6 cut(s) 20, 901, 1003, 1046, 1130, 1192
BsgI GTGCAG 2 cut(s) 697, 878
BshFI GGCC 5 cut(s) 181, 264, 339, 371, 641
BshNI GGYRCC 2 cut(s) 122, 147
BsiHKAI GWGCWC 3 cut(s) 352, 745, 866
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 6 cut(s) 141, 152, 271, 305, 425, 632
BslFI GGGAC 1 cut(s) 424
BslI CCNNNNNNNGG 4 cut(s) 83, 86, 271, 777
BsmAI GTCTC 3 cut(s) 25, 95, 231
BsmFI GGGAC 1 cut(s) 424
BsmI GAATGC 1 cut(s) 731
BsnI GGCC 5 cut(s) 181, 264, 339, 371, 641
Bso31I GGTCTC 1 cut(s) 95
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 3 cut(s) 352, 745, 866
Bsp143I GATC 4 cut(s) 521, 560, 601, 613
BspACI CCGC 5 cut(s) 207, 261, 465, 671, 1208
BspANI GGCC 5 cut(s) 181, 264, 339, 371, 641
BspCNI CTCAG 2 cut(s) 94, 759
BspLI GGNNCC 4 cut(s) 124, 149, 235, 340
BspMAI CTGCAG 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 176
BspPI GGATC 3 cut(s) 516, 555, 596
BspT107I GGYRCC 2 cut(s) 122, 147
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 845
BsrFI RCCGGY 1 cut(s) 424
BsrI ACTGG 3 cut(s) 342, 626, 954
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 3 cut(s) 140, 176, 271
BssMI GATC 4 cut(s) 521, 560, 601, 613
BssNI GRCGYC 1 cut(s) 148
BssT1I CCWWGG 1 cut(s) 176
Bst2UI CCWGG 3 cut(s) 537, 931, 1267
Bst4CI ACNGT 3 cut(s) 109, 225, 514
Bst6I CTCTTC 3 cut(s) 272, 417, 756
BstACI GRCGYC 1 cut(s) 148
BstAPI GCANNNNNTGC 2 cut(s) 969, 1043
BstC8I GCNNGC 5 cut(s) 6, 183, 251, 426, 1203
BstDEI CTNAG 3 cut(s) 81, 240, 746
BstEII GGTNACC 1 cut(s) 296
BstF5I GGATG 5 cut(s) 313, 477, 910, 1067, 1083
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 4 cut(s) 150, 487, 770, 1135
BstKTI GATC 4 cut(s) 524, 563, 604, 616
BstMAI GTCTC 3 cut(s) 25, 95, 231
BstMBI GATC 4 cut(s) 521, 560, 601, 613
BstMWI GCNNNNNNNGC 4 cut(s) 856, 969, 1043, 1139
BstNI CCWGG 3 cut(s) 537, 931, 1267
BstPI GGTNACC 1 cut(s) 296
BstSCI CCNGG 7 cut(s) 139, 140, 150, 270, 535, 929, 1265
BstSFI CTRYAG 4 cut(s) 33, 183, 221, 1162
BstV1I GCAGC 6 cut(s) 20, 901, 1003, 1046, 1130, 1192
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 3 cut(s) 521, 560, 601
BstXI CCANNNNNNTGG 2 cut(s) 308, 393
BstYI RGATCY 3 cut(s) 521, 560, 601
Bsu36I CCTNAGG 1 cut(s) 81
BsuRI GGCC 5 cut(s) 181, 264, 339, 371, 641
BtgZI GCGATG 1 cut(s) 1244
BtsCI GGATG 5 cut(s) 313, 477, 910, 1067, 1083
BtsI GCAGTG 1 cut(s) 864
BtsIMutI CAGTG 1 cut(s) 864
BveI ACCTGC 1 cut(s) 176
Cac8I GCNNGC 5 cut(s) 6, 183, 251, 426, 1203
CaiI CAGNNNCTG 1 cut(s) 997
CfoI GCGC 4 cut(s) 150, 487, 770, 1135
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 338
Cfr9I CCCGGG 1 cut(s) 140
Csp6I GTAC 2 cut(s) 405, 947
CviAII CATG 2 cut(s) 758, 1009
CviQI GTAC 2 cut(s) 405, 947
DdeI CTNAG 3 cut(s) 81, 240, 746
DinI GGCGCC 1 cut(s) 149
DpnI GATC 4 cut(s) 523, 562, 603, 615
DpnII GATC 4 cut(s) 521, 560, 601, 613
EaeI YGGCCR 1 cut(s) 369
Eam1104I CTCTTC 3 cut(s) 272, 417, 756
EarI CTCTTC 3 cut(s) 272, 417, 756
Ecl136II GAGCTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 176
Eco147I AGGCCT 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 352
Eco31I GGTCTC 1 cut(s) 95
Eco53kI GAGCTC 1 cut(s) 350
Eco57I CTGAAG 3 cut(s) 122, 149, 681
Eco81I CCTNAGG 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 140
Eco91I GGTNACC 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 338
EcoO65I GGTNACC 1 cut(s) 296
EcoRII CCWGG 3 cut(s) 535, 929, 1265
EcoT14I CCWWGG 1 cut(s) 176
EcoT38I GRGCYC 1 cut(s) 352
EgeI GGCGCC 1 cut(s) 149
EheI GGCGCC 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 2 cut(s) 761, 1012
FaqI GGGAC 1 cut(s) 424
FatI CATG 2 cut(s) 757, 1008
FauNDI CATATG 2 cut(s) 968, 1114
FbaI TGATCA 1 cut(s) 613
Fnu4HI GCNGC 7 cut(s) 9, 262, 890, 992, 1035, 1119, 1206
FokI GGATG 5 cut(s) 320, 484, 897, 1054, 1070
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 7 cut(s) 9, 262, 890, 992, 1035, 1119, 1206
FspBI CTAG 3 cut(s) 579, 606, 1299
GlaI GCGC 4 cut(s) 149, 486, 769, 1134
GluI GCNGC 7 cut(s) 9, 262, 890, 992, 1035, 1119, 1206
GsuI CTGGAG 2 cut(s) 609, 1288
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 5 cut(s) 181, 264, 339, 371, 641
HapII CCGG 6 cut(s) 141, 152, 271, 305, 425, 632
HhaI GCGC 4 cut(s) 150, 487, 770, 1135
Hin1I GRCGYC 1 cut(s) 148
Hin1II CATG 2 cut(s) 761, 1012
Hin6I GCGC 4 cut(s) 148, 485, 768, 1133
HinP1I GCGC 4 cut(s) 148, 485, 768, 1133
HindIII AAGCTT 1 cut(s) 280
HinfI GANTC 3 cut(s) 38, 592, 1000
HpaII CCGG 6 cut(s) 141, 152, 271, 305, 425, 632
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 2 cut(s) 218, 1153
Hpy188I TCNGA 5 cut(s) 102, 168, 749, 831, 1179
Hpy8I GTNNAC 2 cut(s) 218, 1153
HpyAV CCTTC 1 cut(s) 113
HpyCH4III ACNGT 3 cut(s) 109, 225, 514
HpyF10VI GCNNNNNNNGC 4 cut(s) 856, 969, 1043, 1139
HpyF3I CTNAG 3 cut(s) 81, 240, 746
Hsp92I GRCGYC 1 cut(s) 148
Hsp92II CATG 2 cut(s) 761, 1012
HspAI GCGC 4 cut(s) 148, 485, 768, 1133
KasI GGCGCC 1 cut(s) 147
KroI GCCGGC 1 cut(s) 424
KroNI GCCGGC 1 cut(s) 426
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 4 cut(s) 521, 560, 601, 613
LmnI GCTCC 3 cut(s) 29, 326, 355
Lsp1109I GCAGC 6 cut(s) 20, 901, 1003, 1046, 1130, 1192
LweI GCATC 3 cut(s) 437, 919, 1076
MaeI CTAG 3 cut(s) 579, 606, 1299
MaeIII GTNAC 4 cut(s) 296, 418, 841, 1004
MalI GATC 4 cut(s) 523, 562, 603, 615
MboI GATC 4 cut(s) 521, 560, 601, 613
MflI RGATCY 3 cut(s) 521, 560, 601
MhlI GDGCHC 3 cut(s) 352, 745, 866
MlsI TGGCCA 1 cut(s) 371
MluCI AATT 7 cut(s) 331, 373, 433, 654, 832, 957, 1029
MluNI TGGCCA 1 cut(s) 371
Mly113I GGCGCC 1 cut(s) 148
MlyI GAGTC 1 cut(s) 586
Mox20I TGGCCA 1 cut(s) 371
MroNI GCCGGC 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 654
MscI TGGCCA 1 cut(s) 371
MseI TTAA 4 cut(s) 278, 330, 774, 956
Msp20I TGGCCA 1 cut(s) 371
MspI CCGG 6 cut(s) 141, 152, 271, 305, 425, 632
MspR9I CCNGG 7 cut(s) 141, 142, 152, 272, 537, 931, 1267
Mva1269I GAATGC 1 cut(s) 731
MvaI CCWGG 3 cut(s) 537, 931, 1267
MwoI GCNNNNNNNGC 4 cut(s) 856, 969, 1043, 1139
NaeI GCCGGC 1 cut(s) 426
NarI GGCGCC 1 cut(s) 148
NciI CCSGG 4 cut(s) 141, 142, 152, 272
NdeI CATATG 2 cut(s) 968, 1114
NdeII GATC 4 cut(s) 521, 560, 601, 613
NgoMIV GCCGGC 1 cut(s) 424
NlaIII CATG 2 cut(s) 761, 1012
NlaIV GGNNCC 4 cut(s) 124, 149, 235, 340
NmuCI GTSAC 1 cut(s) 1004
PceI AGGCCT 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 1146
PctI GAATGC 1 cut(s) 731
PdiI GCCGGC 1 cut(s) 426
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 2 cut(s) 38, 1000
PflMI CCANNNNNTGG 1 cut(s) 777
PkrI GCNGC 7 cut(s) 10, 263, 891, 993, 1036, 1120, 1207
PleI GAGTC 1 cut(s) 586
PluTI GGCGCC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 586
Psp124BI GAGCTC 1 cut(s) 352
Psp6I CCWGG 3 cut(s) 535, 929, 1265
PspEI GGTNACC 1 cut(s) 296
PspGI CCWGG 3 cut(s) 535, 929, 1265
PspN4I GGNNCC 4 cut(s) 124, 149, 235, 340
PspPI GGNCC 1 cut(s) 338
PstI CTGCAG 1 cut(s) 187
PstNI CAGNNNCTG 1 cut(s) 997
PsuI RGATCY 3 cut(s) 521, 560, 601
RsaI GTAC 2 cut(s) 406, 948
RsaNI GTAC 2 cut(s) 405, 947
SacI GAGCTC 1 cut(s) 352
SaqAI TTAA 4 cut(s) 278, 330, 774, 956
SatI GCNGC 7 cut(s) 9, 262, 890, 992, 1035, 1119, 1206
Sau3AI GATC 4 cut(s) 521, 560, 601, 613
Sau96I GGNCC 1 cut(s) 338
SbfI CCTGCAGG 1 cut(s) 187
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 7 cut(s) 141, 142, 152, 272, 537, 931, 1267
SdaI CCTGCAGG 1 cut(s) 187
SduI GDGCHC 3 cut(s) 352, 745, 866
SfaNI GCATC 3 cut(s) 437, 919, 1076
SfcI CTRYAG 4 cut(s) 33, 183, 221, 1162
SfoI GGCGCC 1 cut(s) 149
SmaI CCCGGG 1 cut(s) 142
SmlI CTYRAG 3 cut(s) 228, 986, 1124
SmoI CTYRAG 3 cut(s) 228, 986, 1124
Sse8387I CCTGCAGG 1 cut(s) 187
Sse9I AATT 7 cut(s) 331, 373, 433, 654, 832, 957, 1029
SseBI AGGCCT 1 cut(s) 181
SsiI CCGC 5 cut(s) 207, 261, 465, 671, 1208
SspDI GGCGCC 1 cut(s) 147
SspI AATATT 1 cut(s) 1259
SspMI CTAG 3 cut(s) 579, 606, 1299
SstI GAGCTC 1 cut(s) 352
StuI AGGCCT 1 cut(s) 181
StyD4I CCNGG 7 cut(s) 139, 140, 150, 270, 535, 929, 1265
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 3 cut(s) 109, 225, 514
TaqI TCGA 2 cut(s) 518, 1193
TasI AATT 7 cut(s) 331, 373, 433, 654, 832, 957, 1029
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 2 cut(s) 38, 1000
Tru1I TTAA 4 cut(s) 278, 330, 774, 956
Tru9I TTAA 4 cut(s) 278, 330, 774, 956
TscAI CASTG 1 cut(s) 871
TseFI GTSAC 1 cut(s) 1004
TseI GCWGC 6 cut(s) 8, 889, 991, 1034, 1118, 1205
Tsp45I GTSAC 1 cut(s) 1004
TspDTI ATGAA 6 cut(s) 495, 663, 717, 774, 1025, 1163
TspGWI ACGGA 1 cut(s) 553
TspMI CCCGGG 1 cut(s) 140
TspRI CASTG 1 cut(s) 871
Van91I CCANNNNNTGG 1 cut(s) 777
XapI RAATTY 3 cut(s) 433, 654, 832
XmaI CCCGGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 3 cut(s) 579, 606, 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.