MD08G1119900.v1.1

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005136
Physical Location & Seq
Reverse (-)
11020419 .. 11021608
1190 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1119900.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGTTGTGATTAATTGTGTGCAGAAAGAAACTACAACAGTTTCGAGGTCCCTTTCCATGCCTGGACGTAATAAAGTCATTGTAAGATCCGTATATTTTGCAACTCGAAATGAGCAGACTCAAGCAGAACCTAGTGATGATCAAATAACTCCAGTTCCAGTGGAGGCTAATGATGAAGAAATTCCTGAAGAAGAAGCAGTGTGCAGGATATGTCTTGATGTATGTGATGAACAAAATACGCTCAAAATGGAATGTTTTTGCAAAGGTGCTCTTAGACTTCTACATGAAGAGTGCGCCATTAAGTGGTTCAGCACAAAAGGAAACAAGAAATGTGATGTTTGTGGTCAAGAGGTTCAGAATTTACCTGTAACATTGCTTTGGGTGCAGAGCACTGCTCAAAGGGGTAGTGGACAGCAGCACAACCAACAGGGTTTGCATCCAGAAACGATAAGTGCCTGGCAGGACTTTGTGGTAATGTTCTTGATTAGCACAATATGCTATTTCTTCTTCCTTGAGCAGCTACTGGTATGCCATTCTTCTGGTGAAAAAAACTTTAGAACTAAGAATTTGATTTCCGCTAAACTAGAACTTGTTTGTGTCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

202

Amino Acids

22.74

Weight (kDa)

5.29

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 68 - 114 6.8e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 303
AciI CCGC 1 cut(s) 576
AclWI GGATC 1 cut(s) 81
AcsI RAATTY 3 cut(s) 180, 358, 565
AcuI CTGAAG 1 cut(s) 207
AfiI CCNNNNNNNGG 1 cut(s) 303
AjnI CCWGG 2 cut(s) 61, 455
AluBI AGCT 1 cut(s) 520
AluI AGCT 1 cut(s) 520
Alw21I GWGCWC 2 cut(s) 271, 392
AlwI GGATC 1 cut(s) 81
AlwNI CAGNNNCTG 1 cut(s) 523
ApeKI GCWGC 2 cut(s) 415, 517
ApoI RAATTY 3 cut(s) 180, 358, 565
AseI ATTAAT 1 cut(s) 12
Asp700I GAANNNNTTC 1 cut(s) 180
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 48
AsuHPI GGTGA 1 cut(s) 554
AvaII GGWCC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 271, 392
BbvI GCAGC 2 cut(s) 427, 529
BciT130I CCWGG 2 cut(s) 63, 457
BclI TGATCA 1 cut(s) 139
BfaI CTAG 2 cut(s) 132, 584
BisI GCNGC 2 cut(s) 416, 518
BlsI GCNGC 2 cut(s) 417, 519
Bme1390I CCNGG 2 cut(s) 63, 457
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 1 cut(s) 50
BmrFI CCNGG 2 cut(s) 63, 457
BmsI GCATC 1 cut(s) 445
BplI GAGNNNNNCTC 2 cut(s) 379, 411
BpmI CTGGAG 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 105, 533
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse1I ACTGG 3 cut(s) 152, 158, 528
Bse3DI GCAATG 1 cut(s) 371
BseBI CCWGG 2 cut(s) 63, 457
BseGI GGATG 1 cut(s) 436
BseLI CCNNNNNNNGG 1 cut(s) 303
BseMI GCAATG 1 cut(s) 371
BseNI ACTGG 3 cut(s) 152, 158, 528
BseXI GCAGC 2 cut(s) 427, 529
BsgI GTGCAG 3 cut(s) 41, 223, 404
BsiHKAI GWGCWC 2 cut(s) 271, 392
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 34
Bsp1286I GDGCHC 2 cut(s) 271, 392
Bsp143I GATC 2 cut(s) 86, 139
BspACI CCGC 1 cut(s) 576
BspLI GGNNCC 1 cut(s) 50
BspPI GGATC 1 cut(s) 81
BsrDI GCAATG 1 cut(s) 371
BsrI ACTGG 3 cut(s) 152, 158, 528
BssMI GATC 2 cut(s) 86, 139
Bst2UI CCWGG 2 cut(s) 63, 457
Bst4CI ACNGT 1 cut(s) 40
Bst6I CTCTTC 1 cut(s) 282
BstAPI GCANNNNNTGC 1 cut(s) 495
BstDEI CTNAG 2 cut(s) 272, 561
BstF5I GGATG 1 cut(s) 436
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 2 cut(s) 89, 142
BstMBI GATC 2 cut(s) 86, 139
BstMWI GCNNNNNNNGC 2 cut(s) 382, 495
BstNI CCWGG 2 cut(s) 63, 457
BstSCI CCNGG 2 cut(s) 61, 455
BstV1I GCAGC 2 cut(s) 427, 529
BstX2I RGATCY 1 cut(s) 86
BstXI CCANNNNNNTGG 1 cut(s) 539
BstYI RGATCY 1 cut(s) 86
BtsCI GGATG 1 cut(s) 436
BtsI GCAGTG 2 cut(s) 204, 390
BtsIMutI CAGTG 3 cut(s) 165, 204, 390
CaiI CAGNNNCTG 1 cut(s) 523
CfoI GCGC 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 48
CviAII CATG 2 cut(s) 58, 284
CviJI RGCY 2 cut(s) 167, 520
CviKI_1 RGCY 2 cut(s) 167, 520
DdeI CTNAG 2 cut(s) 272, 561
DpnI GATC 2 cut(s) 88, 141
DpnII GATC 2 cut(s) 86, 139
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 207
EcoO109I RGGNCCY 1 cut(s) 48
EcoRII CCWGG 2 cut(s) 61, 455
FaeI CATG 2 cut(s) 61, 287
FaiI YATR 7 cut(s) 59, 94, 211, 223, 285, 496, 529
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 57, 283
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 416, 518
FokI GGATG 1 cut(s) 423
Fsp4HI GCNGC 2 cut(s) 416, 518
FspBI CTAG 2 cut(s) 132, 584
GlaI GCGC 1 cut(s) 295
GluI GCNGC 2 cut(s) 416, 518
GsuI CTGGAG 1 cut(s) 135
HhaI GCGC 1 cut(s) 296
Hin1II CATG 2 cut(s) 61, 287
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HinfI GANTC 1 cut(s) 118
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 1 cut(s) 410
Hpy188I TCNGA 1 cut(s) 357
Hpy188III TCNNGA 6 cut(s) 185, 215, 347, 440, 481, 603
Hpy8I GTNNAC 1 cut(s) 410
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 6 cut(s) 22, 101, 204, 261, 385, 436
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 495
HpyF3I CTNAG 2 cut(s) 272, 561
HpySE526I ACGT 1 cut(s) 67
Hsp92II CATG 2 cut(s) 61, 287
HspAI GCGC 1 cut(s) 294
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 2 cut(s) 86, 139
Lsp1109I GCAGC 2 cut(s) 427, 529
LweI GCATC 1 cut(s) 445
MaeI CTAG 2 cut(s) 132, 584
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 1 cut(s) 367
MalI GATC 2 cut(s) 88, 141
MboI GATC 2 cut(s) 86, 139
MboII GAAGA 7 cut(s) 188, 200, 203, 299, 496, 499, 528
MflI RGATCY 1 cut(s) 86
MhlI GDGCHC 2 cut(s) 271, 392
MluCI AATT 4 cut(s) 13, 180, 358, 565
MlyI GAGTC 1 cut(s) 112
MnlI CCTC 3 cut(s) 39, 157, 343
MroXI GAANNNNTTC 1 cut(s) 180
MseI TTAA 2 cut(s) 12, 300
MspR9I CCNGG 2 cut(s) 63, 457
MvaI CCWGG 2 cut(s) 63, 457
MwoI GCNNNNNNNGC 2 cut(s) 382, 495
NdeII GATC 2 cut(s) 86, 139
NlaIII CATG 2 cut(s) 61, 287
NlaIV GGNNCC 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 180
PflMI CCANNNNNTGG 1 cut(s) 303
PkrI GCNGC 2 cut(s) 417, 519
PleI GAGTC 1 cut(s) 112
PpsI GAGTC 1 cut(s) 112
PpuMI RGGWCCY 1 cut(s) 48
PshBI ATTAAT 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 48
Psp6I CCWGG 2 cut(s) 61, 455
PspGI CCWGG 2 cut(s) 61, 455
PspN4I GGNNCC 1 cut(s) 50
PspPI GGNCC 1 cut(s) 48
PspPPI RGGWCCY 1 cut(s) 48
PstNI CAGNNNCTG 1 cut(s) 523
PsuI RGATCY 1 cut(s) 86
SaqAI TTAA 2 cut(s) 12, 300
SatI GCNGC 2 cut(s) 416, 518
Sau3AI GATC 2 cut(s) 86, 139
Sau96I GGNCC 1 cut(s) 48
SchI GAGTC 1 cut(s) 112
ScrFI CCNGG 2 cut(s) 63, 457
SduI GDGCHC 2 cut(s) 271, 392
SetI ASST 7 cut(s) 50, 70, 133, 268, 354, 367, 522
SfaNI GCATC 1 cut(s) 445
SinI GGWCC 1 cut(s) 48
SmlI CTYRAG 2 cut(s) 120, 512
SmoI CTYRAG 2 cut(s) 120, 512
Sse9I AATT 4 cut(s) 13, 180, 358, 565
SsiI CCGC 1 cut(s) 576
SspMI CTAG 2 cut(s) 132, 584
StyD4I CCNGG 2 cut(s) 61, 455
TaaI ACNGT 1 cut(s) 40
TaiI ACGT 1 cut(s) 70
TaqI TCGA 2 cut(s) 44, 106
TasI AATT 4 cut(s) 13, 180, 358, 565
Tru1I TTAA 2 cut(s) 12, 300
Tru9I TTAA 2 cut(s) 12, 300
TscAI CASTG 3 cut(s) 165, 204, 397
TseI GCWGC 2 cut(s) 415, 517
TspDTI ATGAA 3 cut(s) 189, 243, 300
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 3 cut(s) 165, 204, 397
Van91I CCANNNNNTGG 1 cut(s) 303
VpaK11BI GGWCC 1 cut(s) 48
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 3 cut(s) 180, 358, 565
XmnI GAANNNNTTC 1 cut(s) 180
XspI CTAG 2 cut(s) 132, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.