Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0008488

Physical Location & Seq

Genomic Coordinates Forward (+)

21341696 .. 21350012

Gene Structure 8317 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g17400.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3219 bp

ATGGCGCGACCCCCGAAGCCGGTCTCTTCCGACGAAGCCATGTCCAATTCCGAGGAGGAGGAGCCGATCAACGACGAAGAGGACGAGGAGGAGCTCGAGGCCGTCGCTCGCTCCGCCGACTCCGAAGACGAAGAGGCCGCCGATGACAACTCCCGCGGCTCCGACGACGAGGCGGCCCCGGCTGAGAACGACGACGAGTTGGAAGAGGATGAGGATGGAGGTAATGAAAGCAATGCTGAAGTGAGCAAACGTGAGAAGGCCAGGCTTAAACGCATGGAACAGATGAAGAAGCAAAAAATTCAGGAAATATTAGACGCGCAAAATGCAGCTGTGGATGCTGACATGAACAATAGGGGGAAGGGACGGTTGCAGTATCTTCTGCAGCAAACAGAGTTGTTTGCCCATTTTGCCAAAGGTGATCCGTCTGCATCCCAGAAGAAGGGGAAGGGAAGGGGGCGCCATGCTTCAAAAAAGACTGAAGAAGAAGAAGATGAAGAATGCCTCAAAGAGGAAGAAGATGAATTAACTGGGACCGGAACCACACGGCTCTTGACACAACCTTCATGTGAGAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTACACTTGGGCCTCTATGAAAATGGCATAAATGGAATCCTTGCTGATGAGATGGGTCTTGGTAAAACTTTGCAAACCATATCCTTGTTGGGTTACCTGCATGAATTCAGAGGAATAACTGGCCCTCATATGGTTGTCGCTCCAAAGTCAACCCTTGGAAACTGGATGAATGAGATTCGGCGTTTCTGTCCTGTGTTGCGTGCTGTGAAGTTTCTCGGTAATCCTGATGAAAGAAAACACATACGTGAGGACTTGCTGGTTGCTGGGAAGTTTGATGTTTGTGTTACAAGTTTTGAAATGGCCATCAAAGAAAAGTCTTGCTTACGACGTTTCAGCTGGCGATATATAATCATCGATGAAGCCCATCGAATCAAGAATGAGAATTCACTGCTTTCAAAAACAATGAGGCTGTATAATACCAACTACCGTCTTCTAATCACGGGCACACCGCTTCAGAATAATCTTCATGAACTATGGTCTCTGCTCAACTTTCTTCTGCCAGAGATTTTTAGTTCTGCCGAAACGTTTGATGAATGGTTTCAAATATCTGGTGAAAATGACCAGCAGGAGGTTGTTCAACAGCTTCACAAGGTCCTCCGGCCATTCCTTCTCCGAAGGTTGAAGTCAGATGTTGAGAAAGGTTTGCCTCCAAAAAAGGAAACTATACTTAAAGTAGGCATGTCGCAAATGCAGAAACAGTACTATAAGGCTTTACTGCAGAAAGATCTTGAAGTTGTTAATGCTGGCGGAGAACGTAAGCGTCTTTTGAATATAGCTATGCAGCTTCGTAAATGCTGTAATCATCCATATCTTTTCCAAGGTGCTGAGCCTGGGCCTCCTTATACAACTGGAGACCATCTTATTACAAATGCTGGTAAAATGGTTCTTTTGGACAAGTTACTTCCCAAGTTGAAAGAGCGTGATTCTAGGGTCCTAATATTTTCTCAGATGACAAGGCTGCTAGATATTCTCGAAGATTATCTAATGTTTAAAGGATACCTCTATTGTCGGATTGATGGAAATACCGGTGGTGAAGACCGTGATGCTTCAATCGATGCCTTCAATAAGCCTGGAAGTGAGAAATTTGTATTCTTGCTATCAACTAGAGCTGGAGGACTTGGTATCAATCTTGCTACTGCAGACGTCGTCATCCTTTATGATAGTGATTGGAACCCTCAAGTTGATCTCCAAGCACAAGATCGTGCTCACAGGATTGGTCAGAAGAAAGAAGTTCAAGTGTTCCGGTTCTGCACTGAGTATACCATTGAGGAAAAAGTGATAGAGAGGGCTTATAAAAAGCTTGCCCTTGATGCTCTGGTGATTCAACAAGGACGATTAGCTGAGCAAAAAACTGTTAATAAAGATGAGCTACTTCAAATGGTAAGGTTTGGTGCTGAAATGGTTTTTAGTTCCAAGGATAGCACAATTACCGATGAGGACATTGATAGAATCATTGCGAAAGGAGAAGAGGCAACTGCTGAACTTGATGCGAAGATGAAGAAATTTACCGAGGACGCGATTAAATTTAAGATGGATGATACTGCTGAATTGTACGACTTTGATGACGAGAAGGATGATAAGTTTGACTTCAAGAAGATAGTTAGTGAAAACTGGATTGAACCACCAAAGAGAGAGCGGAAGCGGAATTACTCAGAATCAGAATACTTTAAGCAAACAATGCGCCAAGGTGCTCCAGTAAAACCCAAAGAGCCTCGAATTCCACGCATGCCTCAGTTACATGATTTTCAATTTTTCAACACACAAAGGCTGAGTGAGCTGTATGAAAAGGAAGTACGCTACCTCATGCAAAATCATCAGAAGAATCAGTTGAAGGACACAACTATAGAAGTGGATGAACCAGAAGAAGTTGGAGATCCATTGACTGCTGAAGAACTGGAAGAAAAGGAACGTCTATTAGAAGAGGGATTCTCTTCTTGGAGTAGAAGAGATTTCAATACCTTCATCAGGGCCTGTGAGAAGTATGGGCGAAGTGATATACAAAGCATTGTTGCCGAGATGGAGGGGAAAACAGAGGAGGAAGTAGAAAGATATGCAAAGGTTTTCAAAGAGAGATACAAGGAATTAAATGATTATGATAGGATCATCAAGAACATTGAAAGAGGAGAGGCTCGAATTTCTCGCAAGGATGAGATAATGAAAGCTATTGGGAAAAAGTTGGATCGCTACAAGAATCCATGGCTAGAACTGAAGATCTCATATGGTCAGAACAAGGGGAAGTTGTACAATGAAGAATGTGACCGTTTCATGATATGCATGGTTCACAAGCTTGGGTATGGCAACTGGGATGAGTTGAAAGCAGCATTTCGCACGTCACCCTTGTTTCGGTTTGATTGGTTTGTGAAGTCACGCACAACTCAGGAACTTGCGAGGAGATGTGATACACTAATTCGATTGGTGGAGAAAGAGAACCAAGAAGTTGATGAGAGGGAGAGACAGGCTCGCAAAGAGAAGAAGCTTGCTAAGAACATGACACCATCAAAGCGTGGCACGGGTAGGCAGCCAACTGAGAGCCCCTCTTCCATGAAGAAGAGGAAGCAATTAACTATGGATGATTATGTTAGCACGGGAAAGAGGAGGAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006323 GO:0006325 GO:0006333 GO:0006996 GO:0007275 GO:0008150 GO:0009553 GO:0009561 GO:0009987 GO:0016043 GO:0016584 GO:0022607 GO:0031497 GO:0032501 GO:0032502 GO:0034728 GO:0040034 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044464 GO:0048229 GO:0048506 GO:0048509 GO:0048510 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051276 GO:0065007 GO:0071103 GO:0071824 GO:0071840 GO:0080134 GO:0080135 GO:1900034 GO:1900036

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DBINO HAND Helicase_C SLIDE SNF2_N

Protein Analysis

1073

Amino Acids

124.75

Weight (kDa)

5.59

Isoelectric Point (pI)

45.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNF2-rel_dom	PF00176	209 - 476	3.3e-96	SNF2-related domain
ResIII	PF04851	216 - 355	3.2e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	498 - 611	9.2e-25	Helicase conserved C-terminal domain
HAND	PF09110	759 - 846	1.4e-06	HAND
SLIDE	PF09111	904 - 1014	6.9e-43	SLIDE

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06400 AT3G06400 AT3G06400 AT5G18620 AT5G18620
fragaria_vesca	FvH4_4g17400 FvH4_4g17400
malus_domestica	MD13G1172500.v1.1 MD16G1173900.v1.1
prunus_persica	Prupe.1G140700_v2.0.a1 Prupe.1G140700_v2.0.a1 Prupe.1G140700_v2.0.a1
pyrus_communis	pycom13g14810
rosa_chinensis	RchiOBHm_Chr4g0421321
rosa_laevigata	RLG00000007697
rosa_multiflora	Rmu_sc0015713.1_g000006
rosa_roxburghii	Rroxscaffold_5G00363560
rosa_rugosa	Rorug04G0168900
rosa_samantha	Rh4AG230900 Rh4BG233900 Rh4CG245300 Rh4DG229500
rosa_wichuraiana	Rw4G020300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1908
AatII	GACGTC	1 cut(s)	1761
Acc36I	ACCTGC	1 cut(s)	720
AccB1I	GGYRCC	1 cut(s)	456
AccBSI	CCGCTC	1 cut(s)	2251
AccI	GTMKAC	1 cut(s)	1874
AccII	CGCG	4 cut(s)	7, 156, 317, 2132
AciI	CCGC	9 cut(s)	114, 138, 154, 156, 173, 1064, 1362, 2251, 2257
AclI	AACGTT	1 cut(s)	1139
AclWI	GGATC	4 cut(s)	413, 2483, 2723, 2802
AcoI	YGGCCR	2 cut(s)	915, 1214
AcsI	RAATTY	8 cut(s)	297, 719, 997, 1697, 2117, 2138, 2331, 2748
AcuI	CTGAAG	5 cut(s)	258, 498, 1052, 2523, 2843
AcyI	GRCGYC	2 cut(s)	457, 1758
AfaI	GTAC	4 cut(s)	1316, 2168, 2409, 2858
AfiI	CCNNNNNNNGG	7 cut(s)	19, 439, 508, 745, 1183, 2580, 2958
AgeI	ACCGGT	1 cut(s)	1640
AjiI	CACGTC	1 cut(s)	2946
AjnI	CCWGG	3 cut(s)	260, 1444, 1684
AleI	CACNNNNGTG	1 cut(s)	858
Alw21I	GWGCWC	3 cut(s)	96, 1822, 2308
Alw26I	GTCTC	4 cut(s)	28, 1098, 1461, 3061
AlwI	GGATC	4 cut(s)	413, 2483, 2723, 2802
AlwNI	CAGNNNCTG	1 cut(s)	2586
Ama87I	CYCGRG	1 cut(s)	95
ApeKI	GCWGC	6 cut(s)	326, 382, 1396, 1573, 2933, 3133
ApoI	RAATTY	8 cut(s)	297, 719, 997, 1697, 2117, 2138, 2331, 2748
AsiGI	ACCGGT	1 cut(s)	1640
Asp700I	GAANNNNTTC	1 cut(s)	1152
AspLEI	GCGC	4 cut(s)	7, 319, 459, 2298
AspS9I	GGNCC	8 cut(s)	175, 531, 625, 737, 1207, 1448, 1546, 2583
AsuC2I	CCSGG	1 cut(s)	179
AsuHPI	GGTGA	5 cut(s)	428, 1178, 1658, 1945, 2940
AvaI	CYCGRG	1 cut(s)	95
AvaII	GGWCC	3 cut(s)	531, 1207, 1546
BaeGI	GKGCMC	1 cut(s)	1061
BalI	TGGCCA	1 cut(s)	917
BanI	GGYRCC	1 cut(s)	456
BanII	GRGCYC	2 cut(s)	96, 3149
BarI	GAAGNNNNNNTAC	2 cut(s)	2246, 2278
BbsI	GAAGAC	3 cut(s)	132, 1037, 1656
Bbv12I	GWGCWC	3 cut(s)	96, 1822, 2308
BbvI	GCAGC	6 cut(s)	338, 394, 1408, 1560, 2945, 3145
BccI	CCATC	9 cut(s)	209, 661, 926, 987, 1479, 1625, 2140, 2626, 3118
BceAI	ACGGC	2 cut(s)	86, 560
BciT130I	CCWGG	3 cut(s)	262, 1446, 1686
BciVI	GTATCC	1 cut(s)	1604
BcnI	CCSGG	1 cut(s)	179
BcoDI	GTCTC	4 cut(s)	28, 1098, 1461, 3061
BfaI	CTAG	4 cut(s)	1542, 1577, 1719, 2816
BfmI	CTRYAG	4 cut(s)	380, 1331, 1752, 2457
BfoI	RGCGCY	1 cut(s)	460
BfuAI	ACCTGC	1 cut(s)	720
BfuI	GTATCC	1 cut(s)	1604
BglII	AGATCT	2 cut(s)	1339, 2826
BisI	GCNGC	9 cut(s)	138, 157, 174, 327, 383, 1397, 1574, 2934, 3134
BlpI	GCTNAGC	2 cut(s)	1440, 1956
BlsI	GCNGC	9 cut(s)	139, 158, 175, 328, 384, 1398, 1575, 2935, 3135
BmcAI	AGTACT	1 cut(s)	1316
Bme1390I	CCNGG	4 cut(s)	179, 262, 1446, 1686
Bme18I	GGWCC	3 cut(s)	531, 1207, 1546
BmeT110I	CYCGRG	1 cut(s)	95
BmgBI	CACGTC	1 cut(s)	2946
BmgT120I	GGNCC	8 cut(s)	175, 531, 625, 737, 1207, 1448, 1546, 2583
BmiI	GGNNCC	8 cut(s)	63, 160, 177, 458, 532, 538, 1547, 1787
BmrFI	CCNGG	4 cut(s)	179, 262, 1446, 1686
BmrI	ACTGGG	2 cut(s)	537, 2926
BmsI	GCATC	6 cut(s)	325, 437, 1648, 1660, 1915, 2092
BmuI	ACTGGG	2 cut(s)	537, 2926
BpiI	GAAGAC	3 cut(s)	132, 1037, 1656
BplI	GAGNNNNNCTC	6 cut(s)	2528, 2560, 3058, 3090, 3134, 3166
BpmI	CTGGAG	3 cut(s)	1485, 1746, 2292
Bpu1102I	GCTNAGC	2 cut(s)	1440, 1956
BpuEI	CTTGAG	1 cut(s)	1776
BpuMI	CCSGG	1 cut(s)	179
Bsa29I	ATCGAT	2 cut(s)	969, 1668
BsaAI	YACGTR	1 cut(s)	860
BsaHI	GRCGYC	2 cut(s)	457, 1758
BsaI	GGTCTC	3 cut(s)	28, 1098, 1461
BsaWI	WCCGGW	3 cut(s)	533, 1640, 1857
Bsc4I	CCNNNNNNNGG	7 cut(s)	19, 439, 508, 745, 1183, 2580, 2958
Bse118I	RCCGGY	2 cut(s)	19, 1640
Bse1I	ACTGG	8 cut(s)	532, 739, 782, 1468, 2231, 2309, 2514, 2921
Bse3DI	GCAATG	2 cut(s)	238, 2067
BseBI	CCWGG	3 cut(s)	262, 1446, 1686
BseCI	ATCGAT	2 cut(s)	969, 1668
BseLI	CCNNNNNNNGG	7 cut(s)	19, 439, 508, 745, 1183, 2580, 2958
BseMI	GCAATG	2 cut(s)	238, 2067
BseNI	ACTGG	8 cut(s)	532, 739, 782, 1468, 2231, 2309, 2514, 2921
BseRI	GAGGAG	8 cut(s)	68, 71, 74, 101, 104, 2663, 2751, 3019
BseSI	GKGCMC	1 cut(s)	1061
BseXI	GCAGC	6 cut(s)	338, 394, 1408, 1560, 2945, 3145
BseYI	CCCAGC	1 cut(s)	878
BsgI	GTGCAG	1 cut(s)	1849
Bsh1236I	CGCG	4 cut(s)	7, 156, 317, 2132
BshNI	GGYRCC	1 cut(s)	456
BshTI	ACCGGT	1 cut(s)	1640
BshVI	ATCGAT	2 cut(s)	969, 1668
BsiHKAI	GWGCWC	3 cut(s)	96, 1822, 2308
BsiHKCI	CYCGRG	1 cut(s)	95
BsiSI	CCGG	6 cut(s)	20, 179, 534, 1213, 1641, 1858
BslFI	GGGAC	2 cut(s)	375, 544
BslI	CCNNNNNNNGG	7 cut(s)	19, 439, 508, 745, 1183, 2580, 2958
BsmAI	GTCTC	4 cut(s)	28, 1098, 1461, 3061
BsmFI	GGGAC	2 cut(s)	375, 544
BsmI	GAATGC	1 cut(s)	503
Bso31I	GGTCTC	3 cut(s)	28, 1098, 1461
BsoBI	CYCGRG	1 cut(s)	95
Bsp1286I	GDGCHC	5 cut(s)	96, 1061, 1822, 2308, 3149
Bsp1407I	TGTACA	1 cut(s)	2856
Bsp143I	GATC	9 cut(s)	66, 418, 1339, 1798, 1813, 2488, 2715, 2794, 2826
Bsp1720I	GCTNAGC	2 cut(s)	1440, 1956
Bsp19I	CCATGG	1 cut(s)	2810
BspACI	CCGC	9 cut(s)	114, 138, 154, 156, 173, 1064, 1362, 2251, 2257
BspDI	ATCGAT	2 cut(s)	969, 1668
BspFNI	CGCG	4 cut(s)	7, 156, 317, 2132
BspHI	TCATGA	2 cut(s)	1081, 2880
BspLI	GGNNCC	8 cut(s)	63, 160, 177, 458, 532, 538, 1547, 1787
BspMAI	CTGCAG	3 cut(s)	384, 1335, 1756
BspMI	ACCTGC	1 cut(s)	720
BspPI	GGATC	4 cut(s)	413, 2483, 2723, 2802
BspT107I	GGYRCC	1 cut(s)	456
BspTNI	GGTCTC	3 cut(s)	28, 1098, 1461
BsrBI	CCGCTC	1 cut(s)	2251
BsrDI	GCAATG	2 cut(s)	238, 2067
BsrFI	RCCGGY	2 cut(s)	19, 1640
BsrGI	TGTACA	1 cut(s)	2856
BsrI	ACTGG	8 cut(s)	532, 739, 782, 1468, 2231, 2309, 2514, 2921
BssAI	RCCGGY	2 cut(s)	19, 1640
BssMI	GATC	9 cut(s)	66, 418, 1339, 1798, 1813, 2488, 2715, 2794, 2826
BssNAI	GTATAC	1 cut(s)	1875
BssNI	GRCGYC	2 cut(s)	457, 1758
BssT1I	CCWWGG	5 cut(s)	769, 1432, 2028, 2299, 2810
Bst1107I	GTATAC	1 cut(s)	1875
Bst2UI	CCWGG	3 cut(s)	262, 1446, 1686
Bst4CI	ACNGT	6 cut(s)	366, 1043, 1314, 1655, 1969, 2876
BstACI	GRCGYC	2 cut(s)	457, 1758
BstAPI	GCANNNNNTGC	1 cut(s)	2293
BstAUI	TGTACA	1 cut(s)	2856
BstBAI	YACGTR	1 cut(s)	860
BstC8I	GCNNGC	8 cut(s)	109, 816, 953, 1360, 1917, 2342, 3076, 3093
BstDSI	CCRYGG	2 cut(s)	154, 2810
BstEII	GGTNACC	1 cut(s)	707
BstENI	CCTNNNNNAGG	2 cut(s)	506, 2578
BstFNI	CGCG	4 cut(s)	7, 156, 317, 2132
BstH2I	RGCGCY	1 cut(s)	460
BstHHI	GCGC	4 cut(s)	7, 319, 459, 2298
BstKTI	GATC	9 cut(s)	69, 421, 1342, 1801, 1816, 2491, 2718, 2797, 2829
BstMAI	GTCTC	4 cut(s)	28, 1098, 1461, 3061
BstMBI	GATC	9 cut(s)	66, 418, 1339, 1798, 1813, 2488, 2715, 2794, 2826
BstMWI	GCNNNNNNNGC	8 cut(s)	113, 179, 323, 335, 407, 1925, 2293, 2389
BstNI	CCWGG	3 cut(s)	262, 1446, 1686
BstNSI	RCATGY	2 cut(s)	1297, 2344
BstPI	GGTNACC	1 cut(s)	707
BstSCI	CCNGG	4 cut(s)	177, 260, 1444, 1684
BstSFI	CTRYAG	4 cut(s)	380, 1331, 1752, 2457
BstSLI	GKGCMC	1 cut(s)	1061
BstUI	CGCG	4 cut(s)	7, 156, 317, 2132
BstV1I	GCAGC	6 cut(s)	338, 394, 1408, 1560, 2945, 3145
BstV2I	GAAGAC	3 cut(s)	132, 1037, 1656
BstX2I	RGATCY	3 cut(s)	1339, 2488, 2826
BstYI	RGATCY	3 cut(s)	1339, 2488, 2826
BstZ17I	GTATAC	1 cut(s)	1875
Bsu15I	ATCGAT	2 cut(s)	969, 1668
BsuI	GTATCC	1 cut(s)	1604
BsuTUI	ATCGAT	2 cut(s)	969, 1668
BtgI	CCRYGG	2 cut(s)	154, 2810
BtrI	CACGTC	1 cut(s)	2946
BtsI	GCAGTG	1 cut(s)	1001
BtsIMutI	CAGTG	2 cut(s)	1001, 1866
BveI	ACCTGC	1 cut(s)	720
Cac8I	GCNNGC	8 cut(s)	109, 816, 953, 1360, 1917, 2342, 3076, 3093
CaiI	CAGNNNCTG	1 cut(s)	2586
CciI	TCATGA	2 cut(s)	1081, 2880
CfoI	GCGC	4 cut(s)	7, 319, 459, 2298
Cfr10I	RCCGGY	2 cut(s)	19, 1640
Cfr13I	GGNCC	8 cut(s)	175, 531, 625, 737, 1207, 1448, 1546, 2583
Cfr42I	CCGCGG	1 cut(s)	157
ClaI	ATCGAT	2 cut(s)	969, 1668
CseI	GACGC	3 cut(s)	323, 1364, 2138
Csp6I	GTAC	4 cut(s)	1315, 2167, 2408, 2857
CspAI	ACCGGT	1 cut(s)	1640
CviQI	GTAC	4 cut(s)	1315, 2167, 2408, 2857
DinI	GGCGCC	1 cut(s)	458
DpnI	GATC	9 cut(s)	68, 420, 1341, 1800, 1815, 2490, 2717, 2796, 2828
DpnII	GATC	9 cut(s)	66, 418, 1339, 1798, 1813, 2488, 2715, 2794, 2826
DraI	TTTAAA	1 cut(s)	1606
EaeI	YGGCCR	2 cut(s)	915, 1214
EciI	GGCGGA	2 cut(s)	103, 1377
Ecl136II	GAGCTC	1 cut(s)	94
Eco130I	CCWWGG	5 cut(s)	769, 1432, 2028, 2299, 2810
Eco24I	GRGCYC	2 cut(s)	96, 3149
Eco31I	GGTCTC	3 cut(s)	28, 1098, 1461
Eco47I	GGWCC	3 cut(s)	531, 1207, 1546
Eco53kI	GAGCTC	1 cut(s)	94
Eco57I	CTGAAG	5 cut(s)	258, 498, 1052, 2523, 2843
Eco88I	CYCGRG	1 cut(s)	95
Eco91I	GGTNACC	1 cut(s)	707
EcoICRI	GAGCTC	1 cut(s)	94
EcoNI	CCTNNNNNAGG	2 cut(s)	506, 2578
EcoO109I	RGGNCCY	3 cut(s)	1207, 1546, 2583
EcoO65I	GGTNACC	1 cut(s)	707
EcoRI	GAATTC	3 cut(s)	719, 997, 2331
EcoRII	CCWGG	3 cut(s)	260, 1444, 1684
EcoT14I	CCWWGG	5 cut(s)	769, 1432, 2028, 2299, 2810
EcoT22I	ATGCAT	1 cut(s)	2891
EcoT38I	GRGCYC	2 cut(s)	96, 3149
EgeI	GGCGCC	1 cut(s)	458
EheI	GGCGCC	1 cut(s)	458
ErhI	CCWWGG	5 cut(s)	769, 1432, 2028, 2299, 2810
FalI	AAGNNNNNCTT	4 cut(s)	920, 952, 2186, 2218
FaqI	GGGAC	2 cut(s)	375, 544
FauI	CCCGC	1 cut(s)	161
FauNDI	CATATG	2 cut(s)	744, 2833
FblI	GTMKAC	1 cut(s)	1874
Fnu4HI	GCNGC	9 cut(s)	138, 157, 174, 327, 383, 1397, 1574, 2934, 3134
FriOI	GRGCYC	2 cut(s)	96, 3149
Fsp4HI	GCNGC	9 cut(s)	138, 157, 174, 327, 383, 1397, 1574, 2934, 3134
FspBI	CTAG	4 cut(s)	1542, 1577, 1719, 2816
GlaI	GCGC	4 cut(s)	6, 318, 458, 2297
GluI	GCNGC	9 cut(s)	138, 157, 174, 327, 383, 1397, 1574, 2934, 3134
GsaI	CCCAGC	1 cut(s)	882
GsuI	CTGGAG	3 cut(s)	1485, 1746, 2292
HaeII	RGCGCY	1 cut(s)	460
HapII	CCGG	6 cut(s)	20, 179, 534, 1213, 1641, 1858
HgaI	GACGC	3 cut(s)	323, 1364, 2138
HhaI	GCGC	4 cut(s)	7, 319, 459, 2298
Hin1I	GRCGYC	2 cut(s)	457, 1758
Hin6I	GCGC	4 cut(s)	5, 317, 457, 2296
HinP1I	GCGC	4 cut(s)	5, 317, 457, 2296
HincII	GTYRAC	1 cut(s)	765
HindII	GTYRAC	1 cut(s)	765
HindIII	AAGCTT	3 cut(s)	1913, 2900, 3089
HpaII	CCGG	6 cut(s)	20, 179, 534, 1213, 1641, 1858
HphI	GGTGA	5 cut(s)	428, 1178, 1658, 1945, 2940
Hpy166II	GTNNAC	3 cut(s)	765, 1875, 2896
Hpy8I	GTNNAC	3 cut(s)	765, 1875, 2896
Hpy99I	CGWCG	9 cut(s)	35, 77, 107, 167, 170, 194, 197, 945, 1763
HpyCH4III	ACNGT	6 cut(s)	366, 1043, 1314, 1655, 1969, 2876
HpyCH4IV	ACGT	8 cut(s)	250, 859, 943, 1139, 1369, 1758, 2524, 2945
HpyF10VI	GCNNNNNNNGC	8 cut(s)	113, 179, 323, 335, 407, 1925, 2293, 2389
HpySE526I	ACGT	8 cut(s)	250, 859, 943, 1139, 1369, 1758, 2524, 2945
Hsp92I	GRCGYC	2 cut(s)	457, 1758
HspAI	GCGC	4 cut(s)	5, 317, 457, 2296
KasI	GGCGCC	1 cut(s)	456
KspI	CCGCGG	1 cut(s)	157
Kzo9I	GATC	9 cut(s)	66, 418, 1339, 1798, 1813, 2488, 2715, 2794, 2826
LmnI	GCTCC	6 cut(s)	61, 91, 116, 164, 760, 2311
Lsp1109I	GCAGC	6 cut(s)	338, 394, 1408, 1560, 2945, 3145
LweI	GCATC	6 cut(s)	325, 437, 1648, 1660, 1915, 2092
MaeI	CTAG	4 cut(s)	1542, 1577, 1719, 2816
MaeII	ACGT	8 cut(s)	250, 859, 943, 1139, 1369, 1758, 2524, 2945
MaeIII	GTNAC	8 cut(s)	615, 707, 898, 1512, 2349, 2870, 2946, 2979
MalI	GATC	9 cut(s)	68, 420, 1341, 1800, 1815, 2490, 2717, 2796, 2828
MbiI	CCGCTC	1 cut(s)	2251
MboI	GATC	9 cut(s)	66, 418, 1339, 1798, 1813, 2488, 2715, 2794, 2826
MflI	RGATCY	3 cut(s)	1339, 2488, 2826
MhlI	GDGCHC	5 cut(s)	96, 1061, 1822, 2308, 3149
MlsI	TGGCCA	1 cut(s)	917
MluNI	TGGCCA	1 cut(s)	917
Mly113I	GGCGCC	1 cut(s)	457
MlyI	GAGTC	1 cut(s)	113
MmeI	TCCRAC	6 cut(s)	54, 180, 186, 1604, 2464, 2772
Mox20I	TGGCCA	1 cut(s)	917
Mph1103I	ATGCAT	1 cut(s)	2891
MroXI	GAANNNNTTC	1 cut(s)	1152
MscI	TGGCCA	1 cut(s)	917
MslI	CAYNNNNRTG	1 cut(s)	858
Msp20I	TGGCCA	1 cut(s)	917
MspA1I	CMGCKG	3 cut(s)	156, 329, 951
MspI	CCGG	6 cut(s)	20, 179, 534, 1213, 1641, 1858
MspR9I	CCNGG	4 cut(s)	179, 262, 1446, 1686
Mva1269I	GAATGC	1 cut(s)	503
MvaI	CCWGG	3 cut(s)	262, 1446, 1686
MvnI	CGCG	4 cut(s)	7, 156, 317, 2132
MwoI	GCNNNNNNNGC	8 cut(s)	113, 179, 323, 335, 407, 1925, 2293, 2389
NarI	GGCGCC	1 cut(s)	457
NciI	CCSGG	1 cut(s)	179
NcoI	CCATGG	1 cut(s)	2810
NdeI	CATATG	2 cut(s)	744, 2833
NdeII	GATC	9 cut(s)	66, 418, 1339, 1798, 1813, 2488, 2715, 2794, 2826
NlaIV	GGNNCC	8 cut(s)	63, 160, 177, 458, 532, 538, 1547, 1787
NmeAIII	GCCGAG	1 cut(s)	2653
NmuCI	GTSAC	3 cut(s)	2870, 2946, 2979
NsiI	ATGCAT	1 cut(s)	2891
NspI	RCATGY	2 cut(s)	1297, 2344
OliI	CACNNNNGTG	1 cut(s)	858
PaeI	GCATGC	1 cut(s)	2344
PaeR7I	CTCGAG	1 cut(s)	95
PagI	TCATGA	2 cut(s)	1081, 2880
PcsI	WCGNNNNNNNCGW	1 cut(s)	81
PctI	GAATGC	1 cut(s)	503
PdmI	GAANNNNTTC	1 cut(s)	1152
PflFI	GACNNNGTC	1 cut(s)	1760
PinAI	ACCGGT	1 cut(s)	1640
PkrI	GCNGC	9 cut(s)	139, 158, 175, 328, 384, 1398, 1575, 2935, 3135
PleI	GAGTC	1 cut(s)	113
PluTI	GGCGCC	1 cut(s)	460
PpsI	GAGTC	1 cut(s)	113
Ppu21I	YACGTR	1 cut(s)	860
PpuMI	RGGWCCY	2 cut(s)	1207, 1546
PsiI	TTATAA	1 cut(s)	1908
Psp124BI	GAGCTC	1 cut(s)	96
Psp1406I	AACGTT	1 cut(s)	1139
Psp5II	RGGWCCY	2 cut(s)	1207, 1546
Psp6I	CCWGG	3 cut(s)	260, 1444, 1684
PspEI	GGTNACC	1 cut(s)	707
PspFI	CCCAGC	1 cut(s)	878
PspGI	CCWGG	3 cut(s)	260, 1444, 1684
PspN4I	GGNNCC	8 cut(s)	63, 160, 177, 458, 532, 538, 1547, 1787
PspPI	GGNCC	8 cut(s)	175, 531, 625, 737, 1207, 1448, 1546, 2583
PspPPI	RGGWCCY	2 cut(s)	1207, 1546
PspXI	VCTCGAGB	1 cut(s)	95
PstI	CTGCAG	3 cut(s)	384, 1335, 1756
PstNI	CAGNNNCTG	1 cut(s)	2586
PsuI	RGATCY	3 cut(s)	1339, 2488, 2826
PsyI	GACNNNGTC	1 cut(s)	1760
PvuII	CAGCTG	2 cut(s)	329, 951
RsaI	GTAC	4 cut(s)	1316, 2168, 2409, 2858
RsaNI	GTAC	4 cut(s)	1315, 2167, 2408, 2857
RseI	CAYNNNNRTG	1 cut(s)	858
SacI	GAGCTC	1 cut(s)	96
SacII	CCGCGG	1 cut(s)	157
SatI	GCNGC	9 cut(s)	138, 157, 174, 327, 383, 1397, 1574, 2934, 3134
Sau3AI	GATC	9 cut(s)	66, 418, 1339, 1798, 1813, 2488, 2715, 2794, 2826
Sau96I	GGNCC	8 cut(s)	175, 531, 625, 737, 1207, 1448, 1546, 2583
ScaI	AGTACT	1 cut(s)	1316
SchI	GAGTC	1 cut(s)	113
ScrFI	CCNGG	4 cut(s)	179, 262, 1446, 1686
SduI	GDGCHC	5 cut(s)	96, 1061, 1822, 2308, 3149
SfaNI	GCATC	6 cut(s)	325, 437, 1648, 1660, 1915, 2092
SfcI	CTRYAG	4 cut(s)	380, 1331, 1752, 2457
SfoI	GGCGCC	1 cut(s)	458
Sfr274I	CTCGAG	1 cut(s)	95
Sfr303I	CCGCGG	1 cut(s)	157
SgrBI	CCGCGG	1 cut(s)	157
SinI	GGWCC	3 cut(s)	531, 1207, 1546
SlaI	CTCGAG	1 cut(s)	95
SmiMI	CAYNNNNRTG	1 cut(s)	858
SmlI	CTYRAG	2 cut(s)	95, 1791
SmoI	CTYRAG	2 cut(s)	95, 1791
SphI	GCATGC	1 cut(s)	2344
SsiI	CCGC	9 cut(s)	114, 138, 154, 156, 173, 1064, 1362, 2251, 2257
SspDI	GGCGCC	1 cut(s)	456
SspI	AATATT	2 cut(s)	309, 1554
SspMI	CTAG	4 cut(s)	1542, 1577, 1719, 2816
SstI	GAGCTC	1 cut(s)	96
StyD4I	CCNGG	4 cut(s)	177, 260, 1444, 1684
StyI	CCWWGG	5 cut(s)	769, 1432, 2028, 2299, 2810
TaaI	ACNGT	6 cut(s)	366, 1043, 1314, 1655, 1969, 2876
TaiI	ACGT	8 cut(s)	253, 862, 946, 1142, 1372, 1761, 2527, 2948
TaqI	TCGA	8 cut(s)	96, 969, 982, 1587, 1668, 2329, 2746, 3025
TatI	WGTACW	2 cut(s)	1314, 2856
TauI	GCSGC	3 cut(s)	140, 159, 176
TscAI	CASTG	2 cut(s)	1008, 1873
TseFI	GTSAC	3 cut(s)	2870, 2946, 2979
TseI	GCWGC	6 cut(s)	326, 382, 1396, 1573, 2933, 3133
Tsp45I	GTSAC	3 cut(s)	2870, 2946, 2979
TspGWI	ACGGA	1 cut(s)	411
TspRI	CASTG	2 cut(s)	1008, 1873
Tth111I	GACNNNGTC	1 cut(s)	1760
VpaK11BI	GGWCC	3 cut(s)	531, 1207, 1546
XagI	CCTNNNNNAGG	2 cut(s)	506, 2578
XapI	RAATTY	8 cut(s)	297, 719, 997, 1697, 2117, 2138, 2331, 2748
XceI	RCATGY	2 cut(s)	1297, 2344
XcmI	CCANNNNNNNNNTGG	1 cut(s)	700
XhoI	CTCGAG	1 cut(s)	95
XmiI	GTMKAC	1 cut(s)	1874
XmnI	GAANNNNTTC	1 cut(s)	1152
XspI	CTAG	4 cut(s)	1542, 1577, 1719, 2816
ZraI	GACGTC	1 cut(s)	1759
ZrmI	AGTACT	1 cut(s)	1316
Zsp2I	ATGCAT	1 cut(s)	2891

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.