Rroxscaffold_5G00363560

chromatin-remodeling complex ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008488
Physical Location & Seq
Forward (+)
44830288 .. 44837944
7657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00363560.1

Sequence Viewer

Length: 3210 bp
ATGGCGCGAGGCCCGAAGCCCCAGGTCTCTTCCGACGAAGCCCTATCCAATTCCGAGGAGGAGGAGCCGATCAACGAGGAAGAGGACGAGGAGGAGCTCGAGGCCGTCGCTCGGCCCGCCGACTCCGACGACGAGGACGCGGCCGAGGACAACTCCCGCGGCTCCGATGACGAGGCCGCGCCGGTCGAGAACGACGATGACTTTGAAGAAGAGGATGGAGGTAATGAAAGCAATGCTGAAGTGAGCAAACGCGAGAAGGCCAGGCTTAAACGCATGGAACAAATGAAGAAACAAAAGATTCAGGAAATATTGGATGCACAAAATGCAGCTGTGGATGCTGACATGAACAATCGAGGGAAGGGTCGGTTGCAATATCTTCTGCAGCAAACGGAGTTGTTTGCCCATTTTGCCAAAGGTGACCCGTCTGCATCCCTGAAGAAGGTGAAGGGAAGGGGGCGCCATGCTTCAAAAAAGACTGAAGAGGAAGAGGATGAAGAATGCCTAAAAGAGGAAGAAGATGGTTTATCTGGGACCGGAACGACACGGCTATTGACGCAACCTTCCTGTATTCAGGGGAAGATGAGGGATTACCAACTTGCTGGATTAAACTGGCTTATACGCCTCTATGAAAATGGCATAAATGGAATCCTTGCAGATGAGATGGGTCTTGGTAAAACTTTGCAAACCATATCCTTGTTGGGTTACCTGCATGAATTCAGAGGAATAACCGGCCCTCATATGGTTGTGGCTCCAAAGTCAACCCTTGGAAACTGGATGAATGAGATTCGGCGTTTCTGTCCCATTTTGCGTGCTGTGAAGTTTCTTGGTAATCCTGATGAAAGAAAGCATATACGTGAGGACTTGCTGGTTGCTGGGAAGTTTGATGTTTGTGTTACAAGTTTTGAAATGGCCATCAAAGAGAAGTCTTGCTTACGCCGTTTCAGCTGGCGATATATAATCATTGATGAAGCCCATCGAATCAAGAATGAGAATTCACTTCTTTCGAAAACAATGAGGCTGTATAATACCAACTACCGTCTTCTAATCACGGGCACACCGCTTCAGAATAATCTTCATGAACTATGGTCTCTGCTTAACTTTCTTCTGCCAGAGATATTTAGTTCTGCTGAAACTTTTGATGAATGGTTTCAAATATCTGGTGAAAATGACCAGCAGGAGGTTGTTCAACAGCTTCACAAGGTCCTCCGGCCATTCCTTCTCCGAAGGTTGAAGTCAGATGTTGAGAAAGGTTTGCCTCCGAAAAAAGAAACAATACTCAAAGTAGGCATGTCACAAATGCAGAAACAGTACTATAAGGCTTTACTGCAGAAAGATCTTGAAGTTGTTAATGCTGGTGGAGAACGTAAGCGGCTTCTGAATATAGCTATGCAGCTTCGTAAATGCTGTAATCATCCATATCTTTTCCAAGGTGCTGAGCCTGGGCCTCCTTATACAACTGGAGACCATCTTATCACAAATGCTGGTAAAATGGTGCTTTTGGACAAGTTACTTCCAAAGTTAAAAGAGCGTGATTCTAGGGTCTTAATATTTTCACAGATGACAAGGCTGCTAGATATTCTCGAAGATTATTTAATGTTTAAAGGATACCTTTATTGTCGGATTGATGGAAATACTGGTGGTGAAGACCGTGATGCTTCCATCGATGCCTTCAATAAGCCTGGAAGTGAGAAATTTGTATTCTTGCTATCAACCAGAGCCGGAGGACTTGGTATCAATCTTGCTACTGCAGATGTCAAGAAAGAAGTTCAAGTGTTCCGGTTCTGCACTGAGTATACCATTGAGGAAAAAGTGATTGAGAGGGCTTATAAAAAGCTTGCCCTTGATGCTTTGGTGATTCAACAAGGACGATTAGCTGAGCAAAAAACTGTTAATAAAGATGAGCTACTTCAAATGGTGAGGTTTGGTGCTGAAATGGTTTTTAGTTCCAAGGATAGCACAATTACCGATGAGGACATCGATAGAATCATCGCGAAAGGAGAAGAGGCAACTGCTGAGCTTGATGCGAAGATGAAGAAATTTACTGAGGATGCGATTAAATTTAAGATGGATGATACTGCTGAATTGTATGACTTTGATGATGAGAAGGATGAGAAGTTTGACTTCAAGAAGATTGTTAGTGAAAACTGGATTGAACCACCAAAAAGAGAGCGGAAGCGCAATTACTCAGAATCAGAATACTTTAAGCAAACAATGCGCCAAGGTGCTCCAGCAAAACCCAAAGAGCCTCGAATTCCACGCATGCCTCAATTACATGATTTTCAATTTTTCAACACACAAAGGCTGAGTGAGCTGTATGAAAAGGAAGTACGCTACCTCATGCAAAATCATCAGAAGAATCAGTTGAAGGACACGACTATAGAAGTGGATGAACCAGAAGAAGTTGGAGATCCATTGACTGCTGAAGAGCTGGAAGAAAAGGAACGTCTATTAGAAGAGGGATTCTCTTCATGGAGTAGAAGAGATTTCAATACCTTCATCAGGGCCTGTGAGAAGTATGGGCGGAGTGACATACAAAGCATTGTTGCTGAGATGGACGGGAAAACAGAGGAGGAAGTTGAAAGATATGCTAAGGTTTTCAAAGAGAGATACAAGGAATTAAATGCTTTTGTTAATCAAGCACCCTCTTTAAGCCTCTTCTACCCCCACCCTGAGAGATCTGAGCGAGCTAGGATCATTAAGAACATTGAAAGAGGAGAGGCTAGAATCTCTCGTAAGGATGAGATAATGAAAGCTATTGGGAAGAAGTTGGATCGTTACAAGAATCCATGGCTAGAACTGAAGATCCTGTATGGTCAGAACAAGGGAAAGTTGTACAATGAAGAATGTGACCGTTTCATGATATGCATGGTTCACAAGCTTGGGTATGGAAACTGGGATGAGTTGAAAGCAGCATTTCGCACGTCACCTTTGTTTCGTTTTGATTGGTTTGTGAAGTCGCGCACAACTCAGGAACTTGCTAGGAGGTGTGATACATTAATTCGATTGGTGGAGAAAGAGAACCAAGAAGTTGATGAGAGGAGAGACAAGCTCGCAAAGAGAAGAAGCTTGCTAAGGTTTGATCTCTGCTTCAACATGACACCATCAAAGCGTGGCACGGGGAGGCAGCCAACTGAGAGCCCCTCTTCTTTGAAGAAGAGGAAGCAATTAACTATGGACGATTATGTTAGCACGGGAAAGAGGAGGAGATGA

Protein Analysis

1069

Amino Acids

123.96

Weight (kDa)

5.88

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 193 - 353 6.7e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 197 - 474 7.3e-101 SNF2-related domain
Helicase_C PF00271 496 - 585 5.3e-13 Helicase conserved C-terminal domain
HAND PF09110 732 - 819 9.2e-07 HAND
SLIDE PF09111 897 - 1006 3.1e-42 SLIDE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1827
Acc36I ACCTGC 1 cut(s) 714
AccB1I GGYRCC 1 cut(s) 456
AccBSI CCGCTC 1 cut(s) 2170
AccI GTMKAC 1 cut(s) 1793
AccII CGCG 7 cut(s) 7, 140, 159, 179, 252, 1991, 2959
AciI CCGC 9 cut(s) 117, 140, 157, 159, 177, 1058, 1369, 2170, 2521
AclWI GGATC 4 cut(s) 2402, 2699, 2778, 2797
AcoI YGGCCR 3 cut(s) 141, 909, 1208
AcsI RAATTY 6 cut(s) 713, 991, 1691, 2036, 2057, 2250
AcuI CTGAAG 6 cut(s) 258, 455, 498, 1046, 2442, 2819
AcyI GRCGYC 1 cut(s) 457
AfaI GTAC 3 cut(s) 1310, 2328, 2834
AfiI CCNNNNNNNGG 6 cut(s) 111, 439, 508, 739, 1177, 2499
AjiI CACGTC 1 cut(s) 2922
AjnI CCWGG 4 cut(s) 21, 260, 1438, 1678
AloI GAACNNNNNNTCC 2 cut(s) 2787, 2819
Alw21I GWGCWC 2 cut(s) 99, 2227
Alw26I GTCTC 4 cut(s) 31, 1092, 1455, 3036
AlwI GGATC 4 cut(s) 2402, 2699, 2778, 2797
AlwNI CAGNNNCTG 1 cut(s) 2505
Ama87I CYCGRG 1 cut(s) 98
ApeKI GCWGC 6 cut(s) 326, 382, 1390, 1567, 2909, 3124
ApoI RAATTY 6 cut(s) 713, 991, 1691, 2036, 2057, 2250
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AseI ATTAAT 1 cut(s) 2996
Asp700I GAANNNNTTC 1 cut(s) 1146
AspLEI GCGC 6 cut(s) 7, 181, 459, 2178, 2217, 2961
AspS9I GGNCC 7 cut(s) 11, 114, 531, 731, 1201, 1442, 2502
AsuHPI GGTGA 7 cut(s) 428, 454, 1172, 1652, 1864, 1927, 2916
AsuII TTCGAA 1 cut(s) 1004
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 2 cut(s) 531, 1201
BaeGI GKGCMC 1 cut(s) 1055
BalI TGGCCA 1 cut(s) 911
BanI GGYRCC 1 cut(s) 456
BanII GRGCYC 2 cut(s) 99, 3140
BarI GAAGNNNNNNTAC 2 cut(s) 2165, 2197
BbsI GAAGAC 2 cut(s) 1031, 1650
Bbv12I GWGCWC 2 cut(s) 99, 2227
BbvI GCAGC 6 cut(s) 338, 394, 1402, 1554, 2921, 3136
BceAI ACGGC 3 cut(s) 89, 560, 921
BciT130I CCWGG 4 cut(s) 23, 262, 1440, 1680
BciVI GTATCC 1 cut(s) 1598
BcoDI GTCTC 4 cut(s) 31, 1092, 1455, 3036
BfaI CTAG 6 cut(s) 1536, 1571, 2688, 2721, 2792, 2979
BfmI CTRYAG 4 cut(s) 380, 1325, 1746, 2376
BfoI RGCGCY 1 cut(s) 460
BfuAI ACCTGC 1 cut(s) 714
BfuI GTATCC 1 cut(s) 1598
BglII AGATCT 2 cut(s) 1333, 2675
BlpI GCTNAGC 3 cut(s) 1434, 1875, 2013
BmcAI AGTACT 1 cut(s) 1310
Bme1390I CCNGG 4 cut(s) 23, 262, 1440, 1680
Bme18I GGWCC 2 cut(s) 531, 1201
BmeT110I CYCGRG 1 cut(s) 98
BmgBI CACGTC 1 cut(s) 2922
BmgT120I GGNCC 7 cut(s) 11, 114, 531, 731, 1201, 1442, 2502
BmiI GGNNCC 5 cut(s) 66, 163, 458, 532, 750
BmrFI CCNGG 4 cut(s) 23, 262, 1440, 1680
BmrI ACTGGG 1 cut(s) 2902
BmsI GCATC 8 cut(s) 304, 325, 437, 1642, 1654, 1834, 2011, 2038
BmuI ACTGGG 1 cut(s) 2902
BpiI GAAGAC 2 cut(s) 1031, 1650
BplI GAGNNNNNCTC 8 cut(s) 137, 169, 2447, 2479, 3033, 3065, 3125, 3157
BpmI CTGGAG 2 cut(s) 1479, 2211
Bpu10I CCTNAGC 2 cut(s) 2589, 3071
Bpu1102I GCTNAGC 3 cut(s) 1434, 1875, 2013
Bpu14I TTCGAA 1 cut(s) 1004
Bsa29I ATCGAT 2 cut(s) 1662, 1977
BsaAI YACGTR 1 cut(s) 854
BsaHI GRCGYC 1 cut(s) 457
BsaI GGTCTC 3 cut(s) 31, 1092, 1455
BsaWI WCCGGW 2 cut(s) 533, 1776
Bsc4I CCNNNNNNNGG 6 cut(s) 111, 439, 508, 739, 1177, 2499
Bse118I RCCGGY 2 cut(s) 181, 728
Bse1I ACTGG 6 cut(s) 614, 776, 1462, 1639, 2150, 2897
Bse3DI GCAATG 1 cut(s) 238
BseBI CCWGG 4 cut(s) 23, 262, 1440, 1680
BseCI ATCGAT 2 cut(s) 1662, 1977
BseLI CCNNNNNNNGG 6 cut(s) 111, 439, 508, 739, 1177, 2499
BseMI GCAATG 1 cut(s) 238
BseNI ACTGG 6 cut(s) 614, 776, 1462, 1639, 2150, 2897
BseRI GAGGAG 8 cut(s) 71, 74, 77, 104, 107, 2582, 2727, 3052
BseSI GKGCMC 1 cut(s) 1055
BseX3I CGGCCG 1 cut(s) 141
BseXI GCAGC 6 cut(s) 338, 394, 1402, 1554, 2921, 3136
BseYI CCCAGC 1 cut(s) 872
BsgI GTGCAG 1 cut(s) 1768
Bsh1236I CGCG 7 cut(s) 7, 140, 159, 179, 252, 1991, 2959
Bsh1285I CGRYCG 2 cut(s) 144, 186
BshNI GGYRCC 1 cut(s) 456
BshVI ATCGAT 2 cut(s) 1662, 1977
BsiEI CGRYCG 2 cut(s) 144, 186
BsiHKAI GWGCWC 2 cut(s) 99, 2227
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 6 cut(s) 182, 534, 729, 1207, 1719, 1777
BslFI GGGAC 2 cut(s) 544, 783
BslI CCNNNNNNNGG 6 cut(s) 111, 439, 508, 739, 1177, 2499
BsmAI GTCTC 4 cut(s) 31, 1092, 1455, 3036
BsmFI GGGAC 2 cut(s) 544, 783
BsmI GAATGC 1 cut(s) 503
Bso31I GGTCTC 3 cut(s) 31, 1092, 1455
BsoBI CYCGRG 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 1004
Bsp1286I GDGCHC 4 cut(s) 99, 1055, 2227, 3140
Bsp1407I TGTACA 1 cut(s) 2832
Bsp143I GATC 8 cut(s) 69, 1333, 2407, 2675, 2691, 2770, 2802, 3079
Bsp1720I GCTNAGC 3 cut(s) 1434, 1875, 2013
Bsp19I CCATGG 1 cut(s) 2786
Bsp68I TCGCGA 1 cut(s) 1991
BspACI CCGC 9 cut(s) 117, 140, 157, 159, 177, 1058, 1369, 2170, 2521
BspDI ATCGAT 2 cut(s) 1662, 1977
BspFNI CGCG 7 cut(s) 7, 140, 159, 179, 252, 1991, 2959
BspHI TCATGA 2 cut(s) 1075, 2856
BspLI GGNNCC 5 cut(s) 66, 163, 458, 532, 750
BspMAI CTGCAG 3 cut(s) 384, 1329, 1750
BspMI ACCTGC 1 cut(s) 714
BspPI GGATC 4 cut(s) 2402, 2699, 2778, 2797
BspQI GCTCTTC 1 cut(s) 2418
BspT104I TTCGAA 1 cut(s) 1004
BspT107I GGYRCC 1 cut(s) 456
BspTNI GGTCTC 3 cut(s) 31, 1092, 1455
BsrBI CCGCTC 1 cut(s) 2170
BsrDI GCAATG 1 cut(s) 238
BsrFI RCCGGY 2 cut(s) 181, 728
BsrGI TGTACA 1 cut(s) 2832
BsrI ACTGG 6 cut(s) 614, 776, 1462, 1639, 2150, 2897
BssAI RCCGGY 2 cut(s) 181, 728
BssMI GATC 8 cut(s) 69, 1333, 2407, 2675, 2691, 2770, 2802, 3079
BssNAI GTATAC 1 cut(s) 1794
BssNI GRCGYC 1 cut(s) 457
BssT1I CCWWGG 5 cut(s) 763, 1426, 1947, 2218, 2786
Bst1107I GTATAC 1 cut(s) 1794
Bst2UI CCWGG 4 cut(s) 23, 262, 1440, 1680
Bst4CI ACNGT 5 cut(s) 1037, 1308, 1649, 1888, 2852
BstACI GRCGYC 1 cut(s) 457
BstAPI GCANNNNNTGC 2 cut(s) 323, 2212
BstAUI TGTACA 1 cut(s) 2832
BstBAI YACGTR 1 cut(s) 854
BstBI TTCGAA 1 cut(s) 1004
BstC8I GCNNGC 8 cut(s) 117, 810, 947, 1836, 2261, 2685, 3051, 3068
BstDSI CCRYGG 2 cut(s) 157, 2786
BstEII GGTNACC 2 cut(s) 416, 701
BstENI CCTNNNNNAGG 3 cut(s) 437, 506, 2497
BstFNI CGCG 7 cut(s) 7, 140, 159, 179, 252, 1991, 2959
BstH2I RGCGCY 1 cut(s) 460
BstHHI GCGC 6 cut(s) 7, 181, 459, 2178, 2217, 2961
BstKTI GATC 8 cut(s) 72, 1336, 2410, 2678, 2694, 2773, 2805, 3082
BstMAI GTCTC 4 cut(s) 31, 1092, 1455, 3036
BstMBI GATC 8 cut(s) 69, 1333, 2407, 2675, 2691, 2770, 2802, 3079
BstMCI CGRYCG 2 cut(s) 144, 186
BstMWI GCNNNNNNNGC 9 cut(s) 116, 323, 335, 407, 553, 942, 1844, 2212, 2308
BstNI CCWGG 4 cut(s) 23, 262, 1440, 1680
BstNSI RCATGY 2 cut(s) 1291, 2263
BstPI GGTNACC 2 cut(s) 416, 701
BstSCI CCNGG 4 cut(s) 21, 260, 1438, 1678
BstSFI CTRYAG 4 cut(s) 380, 1325, 1746, 2376
BstSLI GKGCMC 1 cut(s) 1055
BstUI CGCG 7 cut(s) 7, 140, 159, 179, 252, 1991, 2959
BstV1I GCAGC 6 cut(s) 338, 394, 1402, 1554, 2921, 3136
BstV2I GAAGAC 2 cut(s) 1031, 1650
BstX2I RGATCY 4 cut(s) 1333, 2407, 2675, 2802
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 4 cut(s) 1333, 2407, 2675, 2802
BstZ17I GTATAC 1 cut(s) 1794
BstZI CGGCCG 1 cut(s) 141
Bsu15I ATCGAT 2 cut(s) 1662, 1977
BsuI GTATCC 1 cut(s) 1598
BsuTUI ATCGAT 2 cut(s) 1662, 1977
BtgI CCRYGG 2 cut(s) 157, 2786
BtgZI GCGATG 1 cut(s) 1972
BtrI CACGTC 1 cut(s) 2922
BtsIMutI CAGTG 1 cut(s) 1785
BtuMI TCGCGA 1 cut(s) 1991
BveI ACCTGC 1 cut(s) 714
Cac8I GCNNGC 8 cut(s) 117, 810, 947, 1836, 2261, 2685, 3051, 3068
CaiI CAGNNNCTG 1 cut(s) 2505
CciI TCATGA 2 cut(s) 1075, 2856
CfoI GCGC 6 cut(s) 7, 181, 459, 2178, 2217, 2961
Cfr10I RCCGGY 2 cut(s) 181, 728
Cfr13I GGNCC 7 cut(s) 11, 114, 531, 731, 1201, 1442, 2502
Cfr42I CCGCGG 1 cut(s) 160
ClaI ATCGAT 2 cut(s) 1662, 1977
CseI GACGC 2 cut(s) 146, 562
Csp6I GTAC 3 cut(s) 1309, 2327, 2833
CviQI GTAC 3 cut(s) 1309, 2327, 2833
DinI GGCGCC 1 cut(s) 458
DpnI GATC 8 cut(s) 71, 1335, 2409, 2677, 2693, 2772, 2804, 3081
DpnII GATC 8 cut(s) 69, 1333, 2407, 2675, 2691, 2770, 2802, 3079
DraI TTTAAA 1 cut(s) 1600
EaeI YGGCCR 3 cut(s) 141, 909, 1208
EagI CGGCCG 1 cut(s) 141
EciI GGCGGA 1 cut(s) 2536
Ecl136II GAGCTC 1 cut(s) 97
EclXI CGGCCG 1 cut(s) 141
Eco130I CCWWGG 5 cut(s) 763, 1426, 1947, 2218, 2786
Eco24I GRGCYC 2 cut(s) 99, 3140
Eco31I GGTCTC 3 cut(s) 31, 1092, 1455
Eco47I GGWCC 2 cut(s) 531, 1201
Eco52I CGGCCG 1 cut(s) 141
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 6 cut(s) 258, 455, 498, 1046, 2442, 2819
Eco88I CYCGRG 1 cut(s) 98
Eco91I GGTNACC 2 cut(s) 416, 701
EcoICRI GAGCTC 1 cut(s) 97
EcoNI CCTNNNNNAGG 3 cut(s) 437, 506, 2497
EcoO109I RGGNCCY 2 cut(s) 1201, 2502
EcoO65I GGTNACC 2 cut(s) 416, 701
EcoRI GAATTC 3 cut(s) 713, 991, 2250
EcoRII CCWGG 4 cut(s) 21, 260, 1438, 1678
EcoT14I CCWWGG 5 cut(s) 763, 1426, 1947, 2218, 2786
EcoT22I ATGCAT 1 cut(s) 2867
EcoT38I GRGCYC 2 cut(s) 99, 3140
EgeI GGCGCC 1 cut(s) 458
EheI GGCGCC 1 cut(s) 458
ErhI CCWWGG 5 cut(s) 763, 1426, 1947, 2218, 2786
FalI AAGNNNNNCTT 6 cut(s) 914, 946, 1593, 1625, 2105, 2137
FaqI GGGAC 2 cut(s) 544, 783
FauI CCCGC 2 cut(s) 124, 164
FauNDI CATATG 1 cut(s) 738
FblI GTMKAC 1 cut(s) 1793
FriOI GRGCYC 2 cut(s) 99, 3140
FspBI CTAG 6 cut(s) 1536, 1571, 2688, 2721, 2792, 2979
GlaI GCGC 6 cut(s) 6, 180, 458, 2177, 2216, 2960
GsaI CCCAGC 1 cut(s) 876
GsuI CTGGAG 2 cut(s) 1479, 2211
HaeII RGCGCY 1 cut(s) 460
HapII CCGG 6 cut(s) 182, 534, 729, 1207, 1719, 1777
HgaI GACGC 2 cut(s) 146, 562
HhaI GCGC 6 cut(s) 7, 181, 459, 2178, 2217, 2961
Hin1I GRCGYC 1 cut(s) 457
Hin6I GCGC 6 cut(s) 5, 179, 457, 2176, 2215, 2959
HinP1I GCGC 6 cut(s) 5, 179, 457, 2176, 2215, 2959
HincII GTYRAC 1 cut(s) 759
HindII GTYRAC 1 cut(s) 759
HindIII AAGCTT 3 cut(s) 1832, 2876, 3064
HpaII CCGG 6 cut(s) 182, 534, 729, 1207, 1719, 1777
HphI GGTGA 7 cut(s) 428, 454, 1172, 1652, 1864, 1927, 2916
Hpy166II GTNNAC 3 cut(s) 759, 1794, 2872
Hpy8I GTNNAC 3 cut(s) 759, 1794, 2872
Hpy99I CGWCG 5 cut(s) 38, 110, 131, 134, 197
HpyCH4III ACNGT 5 cut(s) 1037, 1308, 1649, 1888, 2852
HpyCH4IV ACGT 4 cut(s) 853, 1363, 2443, 2921
HpyF10VI GCNNNNNNNGC 9 cut(s) 116, 323, 335, 407, 553, 942, 1844, 2212, 2308
HpySE526I ACGT 4 cut(s) 853, 1363, 2443, 2921
Hsp92I GRCGYC 1 cut(s) 457
HspAI GCGC 6 cut(s) 5, 179, 457, 2176, 2215, 2959
KasI GGCGCC 1 cut(s) 456
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 8 cut(s) 69, 1333, 2407, 2675, 2691, 2770, 2802, 3079
LguI GCTCTTC 1 cut(s) 2418
LmnI GCTCC 5 cut(s) 64, 94, 167, 754, 2230
Lsp1109I GCAGC 6 cut(s) 338, 394, 1402, 1554, 2921, 3136
LweI GCATC 8 cut(s) 304, 325, 437, 1642, 1654, 1834, 2011, 2038
MaeI CTAG 6 cut(s) 1536, 1571, 2688, 2721, 2792, 2979
MaeII ACGT 4 cut(s) 853, 1363, 2443, 2921
MaeIII GTNAC 9 cut(s) 416, 701, 892, 1290, 1506, 2525, 2774, 2846, 2922
MalI GATC 8 cut(s) 71, 1335, 2409, 2677, 2693, 2772, 2804, 3081
MbiI CCGCTC 1 cut(s) 2170
MboI GATC 8 cut(s) 69, 1333, 2407, 2675, 2691, 2770, 2802, 3079
MflI RGATCY 4 cut(s) 1333, 2407, 2675, 2802
MhlI GDGCHC 4 cut(s) 99, 1055, 2227, 3140
MlsI TGGCCA 1 cut(s) 911
MluNI TGGCCA 1 cut(s) 911
Mly113I GGCGCC 1 cut(s) 457
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 5 cut(s) 57, 150, 1598, 2383, 2748
Mox20I TGGCCA 1 cut(s) 911
Mph1103I ATGCAT 1 cut(s) 2867
MroXI GAANNNNTTC 1 cut(s) 1146
MscI TGGCCA 1 cut(s) 911
MslI CAYNNNNRTG 1 cut(s) 852
Msp20I TGGCCA 1 cut(s) 911
MspA1I CMGCKG 3 cut(s) 159, 329, 945
MspI CCGG 6 cut(s) 182, 534, 729, 1207, 1719, 1777
MspR9I CCNGG 4 cut(s) 23, 262, 1440, 1680
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 4 cut(s) 23, 262, 1440, 1680
MvnI CGCG 7 cut(s) 7, 140, 159, 179, 252, 1991, 2959
MwoI GCNNNNNNNGC 9 cut(s) 116, 323, 335, 407, 553, 942, 1844, 2212, 2308
NarI GGCGCC 1 cut(s) 457
NcoI CCATGG 1 cut(s) 2786
NdeI CATATG 1 cut(s) 738
NdeII GATC 8 cut(s) 69, 1333, 2407, 2675, 2691, 2770, 2802, 3079
NlaIV GGNNCC 5 cut(s) 66, 163, 458, 532, 750
NmeAIII GCCGAG 2 cut(s) 91, 169
NmuCI GTSAC 5 cut(s) 416, 1290, 2525, 2846, 2922
NruI TCGCGA 1 cut(s) 1991
NsiI ATGCAT 1 cut(s) 2867
NspI RCATGY 2 cut(s) 1291, 2263
NspV TTCGAA 1 cut(s) 1004
PaeI GCATGC 1 cut(s) 2263
PaeR7I CTCGAG 1 cut(s) 98
PagI TCATGA 2 cut(s) 1075, 2856
PciSI GCTCTTC 1 cut(s) 2418
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 1 cut(s) 503
PdmI GAANNNNTTC 1 cut(s) 1146
PleI GAGTC 1 cut(s) 116
PluTI GGCGCC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 116
Ppu21I YACGTR 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 1201
PshBI ATTAAT 1 cut(s) 2996
PsiI TTATAA 1 cut(s) 1827
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 1201
Psp6I CCWGG 4 cut(s) 21, 260, 1438, 1678
PspEI GGTNACC 2 cut(s) 416, 701
PspFI CCCAGC 1 cut(s) 872
PspGI CCWGG 4 cut(s) 21, 260, 1438, 1678
PspN4I GGNNCC 5 cut(s) 66, 163, 458, 532, 750
PspPI GGNCC 7 cut(s) 11, 114, 531, 731, 1201, 1442, 2502
PspPPI RGGWCCY 1 cut(s) 1201
PspXI VCTCGAGB 1 cut(s) 98
PstI CTGCAG 3 cut(s) 384, 1329, 1750
PstNI CAGNNNCTG 1 cut(s) 2505
PsuI RGATCY 4 cut(s) 1333, 2407, 2675, 2802
PvuII CAGCTG 2 cut(s) 329, 945
RruI TCGCGA 1 cut(s) 1991
RsaI GTAC 3 cut(s) 1310, 2328, 2834
RsaNI GTAC 3 cut(s) 1309, 2327, 2833
RseI CAYNNNNRTG 1 cut(s) 852
SacI GAGCTC 1 cut(s) 99
SacII CCGCGG 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 2418
Sau3AI GATC 8 cut(s) 69, 1333, 2407, 2675, 2691, 2770, 2802, 3079
Sau96I GGNCC 7 cut(s) 11, 114, 531, 731, 1201, 1442, 2502
ScaI AGTACT 1 cut(s) 1310
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 4 cut(s) 23, 262, 1440, 1680
SduI GDGCHC 4 cut(s) 99, 1055, 2227, 3140
SfaNI GCATC 8 cut(s) 304, 325, 437, 1642, 1654, 1834, 2011, 2038
SfcI CTRYAG 4 cut(s) 380, 1325, 1746, 2376
SfoI GGCGCC 1 cut(s) 458
Sfr274I CTCGAG 1 cut(s) 98
Sfr303I CCGCGG 1 cut(s) 160
SfuI TTCGAA 1 cut(s) 1004
SgrBI CCGCGG 1 cut(s) 160
SinI GGWCC 2 cut(s) 531, 1201
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
SphI GCATGC 1 cut(s) 2263
SsiI CCGC 9 cut(s) 117, 140, 157, 159, 177, 1058, 1369, 2170, 2521
SspDI GGCGCC 1 cut(s) 456
SspI AATATT 2 cut(s) 309, 1548
SspMI CTAG 6 cut(s) 1536, 1571, 2688, 2721, 2792, 2979
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 4 cut(s) 21, 260, 1438, 1678
StyI CCWWGG 5 cut(s) 763, 1426, 1947, 2218, 2786
TaaI ACNGT 5 cut(s) 1037, 1308, 1649, 1888, 2852
TaiI ACGT 4 cut(s) 856, 1366, 2446, 2924
TatI WGTACW 2 cut(s) 1308, 2832
TauI GCSGC 4 cut(s) 143, 162, 179, 1372
TscAI CASTG 1 cut(s) 1792
TseFI GTSAC 5 cut(s) 416, 1290, 2525, 2846, 2922
TseI GCWGC 6 cut(s) 326, 382, 1390, 1567, 2909, 3124
Tsp45I GTSAC 5 cut(s) 416, 1290, 2525, 2846, 2922
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 1 cut(s) 1792
VpaK11BI GGWCC 2 cut(s) 531, 1201
VspI ATTAAT 1 cut(s) 2996
XagI CCTNNNNNAGG 3 cut(s) 437, 506, 2497
XapI RAATTY 6 cut(s) 713, 991, 1691, 2036, 2057, 2250
XceI RCATGY 2 cut(s) 1291, 2263
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XhoI CTCGAG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 1793
XmnI GAANNNNTTC 1 cut(s) 1146
XspI CTAG 6 cut(s) 1536, 1571, 2688, 2721, 2792, 2979
ZrmI AGTACT 1 cut(s) 1310
Zsp2I ATGCAT 1 cut(s) 2867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.