Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008488

Physical Location & Seq

Genomic Coordinates Reverse (-)

14669398 .. 14677311

Gene Structure 7914 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1173900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3228 bp

ATGGCGAGAGCTTCGAAGCCGCAGGTGTCGTCTGACGAAGCCCCATCCAACGGCTTTAATTCGTCGGAGGAAGAGCCGATTAACGACCAGGAGGAGGAGGAGGTCGACGAGGAGGAGCTCGAAGCCGTGGCTCGCACCGCTGACTCCGACGACGACGAGAACTACCCCGGCTCCGACGATGACATGGTGGCCGATGAGAACGGCGATGATGGCTTGGAGGAGGAAGAGGACGATGAAGCTAGTTTGAGTAATGCTGAAATTGGGAAACGGGAAAAGGCTAGGCTTAGAGACATGCAAAATTTGAAGAGACAAAAGGTTCAAGAAATATTGGATGCACAAAATGCAGCCATTGAGGCTGACATGAATAACAAGGGGAAGGGGCGGTTGAAGTATCTTCTGCGGCAGACAGAGTTATTTGCCCATTTTGCTAAAGGTGACCAGTCTGCATCCCAGAAGAAAGTGAAGGGAAAAGGTCGTCATGCTTCAAAATTAACTGAAGAGGAAGAGGATGAAGAATGCCTTAAGGAGGAAGAAGATGGGTTGGCTGGGGCTGGAACCACGCGGCTGTTGACACAACCTTCTTGTATTCAGGGGAAGATGAGGGATTACCAACTGGCTGGATTGAACTGGCTCATACGACTCTATGAAAATGGTATAAATGGAATCCTTGCAGATGAAATGGGGCTTGGTAAAACATTGCAAACCATCTCCTTATTGGGATACCTGCATGAGTTTAGAGGAATAACTGGCCCTCATATGGTAGTTGCTCCAAAGTCAACTCTTGGAAACTGGATGAATGAGATTCGGCGTTTCTGCCCCATCTTGCGTGCTGTGAAATTTCTTGGTAATCCTGATGAAAGAAAGCATATACGTGAGGACCTTCTGGTTGCCGGGAATTTTGATGTATGTGTCACAAGTTTTGAAATGGCCATCAAAGAGAAGACTTGCTTACGCCGTTTTAGTTGGCGTTATATAATAATTGATGAAGCCCATCGTATTAAGAACGAGAATTCACTGCTTTCAAAAACCATGAGGCTGTATAATACCAACTTCCGCCTCCTAATTACGGGTACACCGCTTCAGAATAATCTTCACGAACTATGGTCTCTGCTTAACTTTCTTCTGCCAGAGATATTTAGTTCTGCTGAAACTTTTGATGAATGGTTTCAAATATCTGGTGAGAACGACCAGCAGGAGGTTGTTCAACAGCTTCACAAGGTCCTCCGGCCATTTCTTCTTCGAAGGTTGAAGTCAGATGTTGAGAAAGGTTTGCCTCCAAAAAAAGAAACAATTCTCAAAGTAGGCATGTCACAAATGCAGAAACAGTACTACAGGGCTTTACTACAAAAAGATCTTGAAGTTGTTAACGCTGGTGGAGAACGTAAACGCCTTCTGAACATAGCAATGCAGCTTCGTAAATGCTGTAATCATCCTTATCTTTTTCAAGGTGCTGAGCCTGGGCCTCCATATACGACCGGAGACCATCTTATCACAAATGCTGGTAAAATGGTTCTTTTGGATAAGTTGCTTCCAAAGTTAAAAGATCGTGATTCTAGGGTCCTAATATTTTCACAGATGACTAGGCTGCTAGATATCCTTGAAGATTATTTAATGTTGCGGGGGTACCTGTATTGTCGCATTGATGGCAATACTGGCGGAGAAGATCGTGATGCTTCCATTGATGCCTTCAACAAGCCTGGAAGTGAGAAATTTGTGTTTTTGCTATCGACCAGAGCTGGAGGACTTGGTATTAATCTTGCCACTGCAGATGTTGTCATCCTTTATGACAGTGATTGGAACCCTCAAGTTGATTTGCAAGCACAAGATCGTGCTCACAGGATTGGTCAGAAGAAAGAAGTTCAAGTGTTCCGGTTCTGCACTGAGTATACTATTGAGGAAAAAGTTATTGAGAGGGCTTATAAAAAGCTTGCTCTTGATGCTTTGGTGATTCAACAAGGGCGATTAGCGGAGCAAAAAACTGTTAATAAAGATGAATTACTTCAAATGGTGAGGTTTGGTGCTGAAATGGTCTTCAGTTCCAAGGATAGCACAATTACAGATGAGGACATTGATAGGATTATTGCTAAAGGAGAAGAGACAACTGCTGAGCTTGATGCAAAGATGAAGAAATTTACTGAAGATGCTATCAAATTTAAGATGGATGATACTGCTGAATTGTATGACTTTGATGATGAAAAGGATGACAAGCTTGATTTCAAGAAAATTGTTAGCGATAACTGGATTGAACCACCAAAAAGAGAGCGGAAGCGCAATTACTCAGAGTCTGAATACTTTAAGCAAACAATGCGCCAAGGTGCTCCAGCAAAACCAAGAGAGCCTCGAATTCCACGCATGCCTCAATTACATGATTTCCAATTTTTCCACACACAAAGGTTGAATGAGCTGTATGAAAAGGAAGTACGTTTCCTCATGCAAACTCATCAGAAGAATCAGTTGAAAGATACAATAGAGGTGGAGGAACCAGAAGAACTTGGAGATCCTTTGACTGCTGAGGAGGTGGAAGAAAAGGAAAGCCTGTTAGAAGAGGGTTTCTCTACTTGGAGTAGAAGAGATTTTAATACCTTCATTAGGGCCTGTGAGAAGTATGGCCGTAATGACATAAAAAGCATTGCTGCCGAGATGGAAGGGAAAACAGAGGAGGAAGTTGAAAGATACGCAAAGATTTTTAAAGAAAGATACAAGGAGTTAAATGATTATGATAGGATCATTAAGAACATTGAAAGAGGTGAGGCTAGAATTTCCCGCAAAGATGAGATCATGAAAGCCATTGGGAAGAAGTTGGATCGTTACAAGAATCCATGGCTGGAATTGAAGATCCAGTATGGTCAGAACAAGGGGAAGTTGTACAATGAAGAATGCGATCGTTTCATGTTATGCATGGTTCACAAACTTGGGTATGGGAATTGGGACGAGTTGAAGGCAGAATTCCGCACATCACCCTTGTTTCGTTTTGATTGGTTTGTGAAGTCTCGCACAACTCAGGAACTCGCTAGGAGGTGTGATACAATAATTAGGCTGGTGGAGAAAGAGAACCAAGAATACGACGAGAGGGAGAGGCAGGCCCGCAAAGAGAAGAAGCTTGCTAAGAGCATGGCGCCATCGAAGCGGGCCATGGGCAGGCAGCCGACTGAGAGCCCAAATTCCGGAAAGAAGAGGAAGCAACTAACCATGGATGATTATGTCAACTCGGGAAAAAAGAGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006323 GO:0006325 GO:0006333 GO:0006996 GO:0007275 GO:0008150 GO:0009553 GO:0009561 GO:0009987 GO:0016043 GO:0016584 GO:0022607 GO:0031497 GO:0032501 GO:0032502 GO:0034728 GO:0040034 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044464 GO:0048229 GO:0048506 GO:0048509 GO:0048510 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051276 GO:0065007 GO:0071103 GO:0071824 GO:0071840 GO:0080134 GO:0080135 GO:1900034 GO:1900036

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DBINO HAND Helicase_C SLIDE SNF2_N

Protein Analysis

1076

Amino Acids

124.69

Weight (kDa)

5.58

Isoelectric Point (pI)

43.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	199 - 359	3.8e-09	Type III restriction enzyme, res subunit
SNF2-rel_dom	PF00176	203 - 480	4.9e-99	SNF2-related domain
Helicase_C	PF00271	502 - 615	1.7e-24	Helicase conserved C-terminal domain
HAND	PF09110	763 - 844	3.2e-06	HAND
SLIDE	PF09111	907 - 1017	5.5e-43	SLIDE

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06400 AT3G06400 AT3G06400 AT5G18620 AT5G18620
fragaria_vesca	FvH4_4g17400 FvH4_4g17400
malus_domestica	MD13G1172500.v1.1 MD16G1173900.v1.1
prunus_persica	Prupe.1G140700_v2.0.a1 Prupe.1G140700_v2.0.a1 Prupe.1G140700_v2.0.a1
pyrus_communis	pycom13g14810
rosa_chinensis	RchiOBHm_Chr4g0421321
rosa_laevigata	RLG00000007697
rosa_multiflora	Rmu_sc0015713.1_g000006
rosa_roxburghii	Rroxscaffold_5G00363560
rosa_rugosa	Rorug04G0168900
rosa_samantha	Rh4AG230900 Rh4BG233900 Rh4CG245300 Rh4DG229500
rosa_wichuraiana	Rw4G020300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1920
AarI	CACCTGC	1 cut(s)	13
Acc36I	ACCTGC	2 cut(s)	13, 732
Acc65I	GGTACC	1 cut(s)	1623
AccB1I	GGYRCC	2 cut(s)	1623, 3115
AccBSI	CCGCTC	1 cut(s)	2263
AccI	GTMKAC	2 cut(s)	105, 1886
AccII	CGCG	1 cut(s)	562
AccIII	TCCGGA	1 cut(s)	3164
AclWI	GGATC	4 cut(s)	2492, 2732, 2811, 2830
AcoI	YGGCCR	4 cut(s)	189, 927, 1226, 2608
AcuI	CTGAAG	4 cut(s)	516, 1064, 2017, 2157
AcyI	GRCGYC	1 cut(s)	3116
AfaI	GTAC	5 cut(s)	1072, 1328, 1625, 2421, 2867
AfiI	CCNNNNNNNGG	9 cut(s)	50, 94, 526, 757, 1066, 1195, 2589, 3138, 3164
AflII	CTTAAG	1 cut(s)	521
AjnI	CCWGG	3 cut(s)	87, 1456, 1696
AjuI	GAANNNNNNNTTGG	2 cut(s)	669, 701
Alw21I	GWGCWC	3 cut(s)	120, 1834, 2320
Alw26I	GTCTC	6 cut(s)	282, 301, 1110, 1473, 2090, 2994
AlwI	GGATC	4 cut(s)	2492, 2732, 2811, 2830
AlwNI	CAGNNNCTG	1 cut(s)	2285
Ama87I	CYCGRG	1 cut(s)	3208
Aor13HI	TCCGGA	1 cut(s)	3164
AoxI	GGCC	9 cut(s)	189, 748, 927, 1226, 1460, 2592, 2608, 3081, 3129
ApeKI	GCWGC	5 cut(s)	344, 1408, 1585, 2633, 3142
AseI	ATTAAT	1 cut(s)	1752
Asp700I	GAANNNNTTC	3 cut(s)	1164, 1290, 1998
Asp718I	GGTACC	1 cut(s)	1623
AspLEI	GCGC	3 cut(s)	2271, 2310, 3118
AspS9I	GGNCC	8 cut(s)	749, 877, 1219, 1460, 1558, 2592, 3082, 3129
AsuC2I	CCSGG	2 cut(s)	168, 892
AsuHPI	GGTGA	6 cut(s)	446, 1190, 1957, 2020, 2759, 2949
AsuII	TTCGAA	2 cut(s)	14, 1240
AvaI	CYCGRG	1 cut(s)	3208
AvaII	GGWCC	3 cut(s)	877, 1219, 1558
BalI	TGGCCA	1 cut(s)	929
BanI	GGYRCC	2 cut(s)	1623, 3115
BanII	GRGCYC	2 cut(s)	120, 3158
BarI	GAAGNNNNNNTAC	2 cut(s)	2258, 2290
BbsI	GAAGAC	2 cut(s)	947, 2023
Bbv12I	GWGCWC	3 cut(s)	120, 1834, 2320
BbvCI	CCTCAGC	1 cut(s)	2511
BbvI	GCAGC	5 cut(s)	356, 1420, 1572, 2620, 3154
BceAI	ACGGC	5 cut(s)	67, 110, 217, 939, 2595
BciT130I	CCWGG	3 cut(s)	89, 1458, 1698
BciVI	GTATCC	1 cut(s)	713
BcnI	CCSGG	2 cut(s)	168, 892
BcoDI	GTCTC	6 cut(s)	282, 301, 1110, 1473, 2090, 2994
BfaI	CTAG	7 cut(s)	240, 279, 1554, 1581, 1589, 2754, 3012
BfmI	CTRYAG	2 cut(s)	1330, 1764
BfoI	RGCGCY	1 cut(s)	3119
BfrI	CTTAAG	1 cut(s)	521
BfuAI	ACCTGC	2 cut(s)	13, 732
BfuI	GTATCC	1 cut(s)	713
BglI	GCCNNNNNGGC	1 cut(s)	353
BglII	AGATCT	1 cut(s)	1351
BisI	GCNGC	8 cut(s)	20, 345, 401, 563, 1409, 1586, 2634, 3143
BlpI	GCTNAGC	2 cut(s)	1452, 2106
BlsI	GCNGC	8 cut(s)	21, 346, 402, 564, 1410, 1587, 2635, 3144
BmcAI	AGTACT	1 cut(s)	1328
Bme1390I	CCNGG	5 cut(s)	89, 168, 892, 1458, 1698
Bme18I	GGWCC	3 cut(s)	877, 1219, 1558
BmeT110I	CYCGRG	1 cut(s)	3208
BmgT120I	GGNCC	8 cut(s)	749, 877, 1219, 1460, 1558, 2592, 3082, 3129
BmiI	GGNNCC	7 cut(s)	172, 556, 1559, 1625, 1799, 2481, 3117
BmrFI	CCNGG	5 cut(s)	89, 168, 892, 1458, 1698
BmsI	GCATC	7 cut(s)	322, 455, 1660, 1672, 1927, 2104, 2131
BpiI	GAAGAC	2 cut(s)	947, 2023
BplI	GAGNNNNNCTC	2 cut(s)	2537, 2569
BpmI	CTGGAG	2 cut(s)	1758, 2304
Bpu10I	CCTNAGC	1 cut(s)	2511
Bpu1102I	GCTNAGC	2 cut(s)	1452, 2106
Bpu14I	TTCGAA	2 cut(s)	14, 1240
BpuEI	CTTGAG	1 cut(s)	1788
BpuMI	CCSGG	2 cut(s)	168, 892
BsaAI	YACGTR	1 cut(s)	872
BsaHI	GRCGYC	1 cut(s)	3116
BsaI	GGTCTC	2 cut(s)	1110, 1473
BsaJI	CCNNGG	8 cut(s)	126, 166, 1457, 2040, 2311, 2819, 3132, 3189
BsaWI	WCCGGW	3 cut(s)	1475, 1869, 3164
BsaXI	ACNNNNNCTCC	2 cut(s)	155, 185
Bsc4I	CCNNNNNNNGG	9 cut(s)	50, 94, 526, 757, 1066, 1195, 2589, 3138, 3164
Bse1I	ACTGG	8 cut(s)	439, 618, 632, 751, 794, 1657, 2243, 2839
Bse3DI	GCAATG	3 cut(s)	695, 1410, 2628
BseAI	TCCGGA	1 cut(s)	3164
BseBI	CCWGG	3 cut(s)	89, 1458, 1698
BseDI	CCNNGG	8 cut(s)	126, 166, 1457, 2040, 2311, 2819, 3132, 3189
BseLI	CCNNNNNNNGG	9 cut(s)	50, 94, 526, 757, 1066, 1195, 2589, 3138, 3164
BseMI	GCAATG	3 cut(s)	695, 1410, 2628
BseMII	CTCAG	7 cut(s)	1443, 1872, 2097, 2292, 2502, 3014, 3141
BseNI	ACTGG	8 cut(s)	439, 618, 632, 751, 794, 1657, 2243, 2839
BseRI	GAGGAG	8 cut(s)	107, 110, 113, 125, 128, 233, 2528, 2672
BseXI	GCAGC	5 cut(s)	356, 1420, 1572, 2620, 3154
BseYI	CCCAGC	1 cut(s)	545
BsgI	GTGCAG	1 cut(s)	1861
Bsh1236I	CGCG	1 cut(s)	562
Bsh1285I	CGRYCG	2 cut(s)	1476, 2884
BshFI	GGCC	9 cut(s)	191, 750, 929, 1228, 1462, 2594, 2610, 3083, 3131
BshNI	GGYRCC	2 cut(s)	1623, 3115
BsiEI	CGRYCG	2 cut(s)	1476, 2884
BsiHKAI	GWGCWC	3 cut(s)	120, 1834, 2320
BsiHKCI	CYCGRG	1 cut(s)	3208
BsiSI	CCGG	6 cut(s)	168, 891, 1225, 1476, 1870, 3165
BslFI	GGGAC	1 cut(s)	2942
BslI	CCNNNNNNNGG	9 cut(s)	50, 94, 526, 757, 1066, 1195, 2589, 3138, 3164
BsmAI	GTCTC	6 cut(s)	282, 301, 1110, 1473, 2090, 2994
BsmFI	GGGAC	1 cut(s)	2942
BsmI	GAATGC	2 cut(s)	521, 2882
BsnI	GGCC	9 cut(s)	191, 750, 929, 1228, 1462, 2594, 2610, 3083, 3131
Bso31I	GGTCTC	2 cut(s)	1110, 1473
BsoBI	CYCGRG	1 cut(s)	3208
Bsp119I	TTCGAA	2 cut(s)	14, 1240
Bsp1286I	GDGCHC	4 cut(s)	120, 1834, 2320, 3158
Bsp13I	TCCGGA	1 cut(s)	3164
Bsp1407I	TGTACA	1 cut(s)	2865
Bsp1720I	GCTNAGC	2 cut(s)	1452, 2106
Bsp19I	CCATGG	3 cut(s)	2819, 3132, 3189
BspANI	GGCC	9 cut(s)	191, 750, 929, 1228, 1462, 2594, 2610, 3083, 3131
BspCNI	CTCAG	7 cut(s)	1444, 1873, 2098, 2291, 2503, 3013, 3142
BspEI	TCCGGA	1 cut(s)	3164
BspFNI	CGCG	1 cut(s)	562
BspHI	TCATGA	1 cut(s)	2778
BspLI	GGNNCC	7 cut(s)	172, 556, 1559, 1625, 1799, 2481, 3117
BspMAI	CTGCAG	1 cut(s)	1768
BspMI	ACCTGC	2 cut(s)	13, 732
BspPI	GGATC	4 cut(s)	2492, 2732, 2811, 2830
BspQI	GCTCTTC	1 cut(s)	66
BspT104I	TTCGAA	2 cut(s)	14, 1240
BspT107I	GGYRCC	2 cut(s)	1623, 3115
BspTI	CTTAAG	1 cut(s)	521
BspTNI	GGTCTC	2 cut(s)	1110, 1473
BsrBI	CCGCTC	1 cut(s)	2263
BsrDI	GCAATG	3 cut(s)	695, 1410, 2628
BsrGI	TGTACA	1 cut(s)	2865
BsrI	ACTGG	8 cut(s)	439, 618, 632, 751, 794, 1657, 2243, 2839
BssECI	CCNNGG	8 cut(s)	126, 166, 1457, 2040, 2311, 2819, 3132, 3189
BssNAI	GTATAC	1 cut(s)	1887
BssNI	GRCGYC	1 cut(s)	3116
BssT1I	CCWWGG	5 cut(s)	2040, 2311, 2819, 3132, 3189
Bst1107I	GTATAC	1 cut(s)	1887
Bst2UI	CCWGG	3 cut(s)	89, 1458, 1698
Bst4CI	ACNGT	3 cut(s)	1326, 1790, 1981
Bst6I	CTCTTC	9 cut(s)	66, 219, 299, 492, 498, 2088, 2538, 2563, 3167
BstACI	GRCGYC	1 cut(s)	3116
BstAFI	CTTAAG	1 cut(s)	521
BstAPI	GCANNNNNTGC	2 cut(s)	341, 2305
BstAUI	TGTACA	1 cut(s)	2865
BstBAI	YACGTR	1 cut(s)	872
BstBI	TTCGAA	2 cut(s)	14, 1240
BstDEI	CTNAG	9 cut(s)	284, 1452, 1881, 2106, 2278, 2511, 3000, 3105, 3150
BstDSI	CCRYGG	4 cut(s)	126, 2819, 3132, 3189
BstEII	GGTNACC	1 cut(s)	434
BstENI	CCTNNNNNAGG	2 cut(s)	524, 2587
BstFNI	CGCG	1 cut(s)	562
BstH2I	RGCGCY	1 cut(s)	3119
BstHHI	GCGC	3 cut(s)	2271, 2310, 3118
BstMAI	GTCTC	6 cut(s)	282, 301, 1110, 1473, 2090, 2994
BstMCI	CGRYCG	2 cut(s)	1476, 2884
BstNI	CCWGG	3 cut(s)	89, 1458, 1698
BstNSI	RCATGY	3 cut(s)	295, 1309, 2356
BstPI	GGTNACC	1 cut(s)	434
BstSCI	CCNGG	5 cut(s)	87, 166, 890, 1456, 1696
BstSFI	CTRYAG	2 cut(s)	1330, 1764
BstUI	CGCG	1 cut(s)	562
BstV1I	GCAGC	5 cut(s)	356, 1420, 1572, 2620, 3154
BstV2I	GAAGAC	2 cut(s)	947, 2023
BstX2I	RGATCY	3 cut(s)	1351, 2497, 2835
BstXI	CCANNNNNNTGG	1 cut(s)	617
BstYI	RGATCY	3 cut(s)	1351, 2497, 2835
BstZ17I	GTATAC	1 cut(s)	1887
BsuI	GTATCC	1 cut(s)	713
BsuRI	GGCC	9 cut(s)	191, 750, 929, 1228, 1462, 2594, 2610, 3083, 3131
BtgI	CCRYGG	4 cut(s)	126, 2819, 3132, 3189
BtgZI	GCGATG	1 cut(s)	219
BtsI	GCAGTG	2 cut(s)	1013, 1761
BtsIMutI	CAGTG	4 cut(s)	1013, 1761, 1795, 1878
BveI	ACCTGC	2 cut(s)	13, 732
CaiI	CAGNNNCTG	1 cut(s)	2285
CciI	TCATGA	1 cut(s)	2778
CfoI	GCGC	3 cut(s)	2271, 2310, 3118
Cfr13I	GGNCC	8 cut(s)	749, 877, 1219, 1460, 1558, 2592, 3082, 3129
Csp6I	GTAC	5 cut(s)	1071, 1327, 1624, 2420, 2866
CspCI	CAANNNNNGTGG	2 cut(s)	2454, 2489
CviQI	GTAC	5 cut(s)	1071, 1327, 1624, 2420, 2866
DdeI	CTNAG	9 cut(s)	284, 1452, 1881, 2106, 2278, 2511, 3000, 3105, 3150
DinI	GGCGCC	1 cut(s)	3117
DraI	TTTAAA	1 cut(s)	2689
EaeI	YGGCCR	4 cut(s)	189, 927, 1226, 2608
Eam1104I	CTCTTC	9 cut(s)	66, 219, 299, 492, 498, 2088, 2538, 2563, 3167
EarI	CTCTTC	9 cut(s)	66, 219, 299, 492, 498, 2088, 2538, 2563, 3167
EciI	GGCGGA	2 cut(s)	1043, 1671
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	5 cut(s)	2040, 2311, 2819, 3132, 3189
Eco24I	GRGCYC	2 cut(s)	120, 3158
Eco31I	GGTCTC	2 cut(s)	1110, 1473
Eco32I	GATATC	1 cut(s)	1594
Eco47I	GGWCC	3 cut(s)	877, 1219, 1558
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	4 cut(s)	516, 1064, 2017, 2157
Eco88I	CYCGRG	1 cut(s)	3208
Eco91I	GGTNACC	1 cut(s)	434
EcoICRI	GAGCTC	1 cut(s)	118
EcoNI	CCTNNNNNAGG	2 cut(s)	524, 2587
EcoO109I	RGGNCCY	4 cut(s)	877, 1219, 1558, 2592
EcoO65I	GGTNACC	1 cut(s)	434
EcoRI	GAATTC	3 cut(s)	1009, 2343, 2945
EcoRII	CCWGG	3 cut(s)	87, 1456, 1696
EcoRV	GATATC	1 cut(s)	1594
EcoT14I	CCWWGG	5 cut(s)	2040, 2311, 2819, 3132, 3189
EcoT22I	ATGCAT	1 cut(s)	2900
EcoT38I	GRGCYC	2 cut(s)	120, 3158
EgeI	GGCGCC	1 cut(s)	3117
EheI	GGCGCC	1 cut(s)	3117
ErhI	CCWWGG	5 cut(s)	2040, 2311, 2819, 3132, 3189
FalI	AAGNNNNNCTT	4 cut(s)	504, 536, 932, 964
FaqI	GGGAC	1 cut(s)	2942
FauI	CCCGC	4 cut(s)	1611, 2771, 3092, 3120
FauNDI	CATATG	1 cut(s)	756
FblI	GTMKAC	2 cut(s)	105, 1886
Fnu4HI	GCNGC	8 cut(s)	20, 345, 401, 563, 1409, 1586, 2634, 3143
FriOI	GRGCYC	2 cut(s)	120, 3158
Fsp4HI	GCNGC	8 cut(s)	20, 345, 401, 563, 1409, 1586, 2634, 3143
FspBI	CTAG	7 cut(s)	240, 279, 1554, 1581, 1589, 2754, 3012
GlaI	GCGC	3 cut(s)	2270, 2309, 3117
GluI	GCNGC	8 cut(s)	20, 345, 401, 563, 1409, 1586, 2634, 3143
GsaI	CCCAGC	1 cut(s)	549
GsuI	CTGGAG	2 cut(s)	1758, 2304
HaeII	RGCGCY	1 cut(s)	3119
HaeIII	GGCC	9 cut(s)	191, 750, 929, 1228, 1462, 2594, 2610, 3083, 3131
HapII	CCGG	6 cut(s)	168, 891, 1225, 1476, 1870, 3165
HhaI	GCGC	3 cut(s)	2271, 2310, 3118
Hin1I	GRCGYC	1 cut(s)	3116
Hin6I	GCGC	3 cut(s)	2269, 2308, 3116
HinP1I	GCGC	3 cut(s)	2269, 2308, 3116
HincII	GTYRAC	5 cut(s)	106, 570, 777, 1366, 3205
HindII	GTYRAC	5 cut(s)	106, 570, 777, 1366, 3205
HindIII	AAGCTT	3 cut(s)	1925, 2207, 3098
HinfI	GANTC	9 cut(s)	143, 639, 663, 802, 1550, 1948, 2282, 2449, 2815
HpaI	GTTAAC	1 cut(s)	1366
HpaII	CCGG	6 cut(s)	168, 891, 1225, 1476, 1870, 3165
HphI	GGTGA	6 cut(s)	446, 1190, 1957, 2020, 2759, 2949
Hpy166II	GTNNAC	9 cut(s)	106, 570, 777, 1073, 1366, 1385, 1887, 2905, 3205
Hpy8I	GTNNAC	9 cut(s)	106, 570, 777, 1073, 1366, 1385, 1887, 2905, 3205
Hpy99I	CGWCG	7 cut(s)	67, 110, 152, 155, 158, 179, 3068
HpyCH4III	ACNGT	3 cut(s)	1326, 1790, 1981
HpyCH4IV	ACGT	3 cut(s)	871, 1381, 2422
HpyF3I	CTNAG	9 cut(s)	284, 1452, 1881, 2106, 2278, 2511, 3000, 3105, 3150
HpySE526I	ACGT	3 cut(s)	871, 1381, 2422
Hsp92I	GRCGYC	1 cut(s)	3116
HspAI	GCGC	3 cut(s)	2269, 2308, 3116
KasI	GGCGCC	1 cut(s)	3115
Kpn2I	TCCGGA	1 cut(s)	3164
KpnI	GGTACC	1 cut(s)	1627
KspAI	GTTAAC	1 cut(s)	1366
LguI	GCTCTTC	1 cut(s)	66
LmnI	GCTCC	5 cut(s)	115, 176, 772, 1969, 2323
Lsp1109I	GCAGC	5 cut(s)	356, 1420, 1572, 2620, 3154
LweI	GCATC	7 cut(s)	322, 455, 1660, 1672, 1927, 2104, 2131
MaeI	CTAG	7 cut(s)	240, 279, 1554, 1581, 1589, 2754, 3012
MaeII	ACGT	3 cut(s)	871, 1381, 2422
MaeIII	GTNAC	4 cut(s)	434, 910, 1308, 2807
MbiI	CCGCTC	1 cut(s)	2263
MflI	RGATCY	3 cut(s)	1351, 2497, 2835
MhlI	GDGCHC	4 cut(s)	120, 1834, 2320, 3158
MlsI	TGGCCA	1 cut(s)	929
MluNI	TGGCCA	1 cut(s)	929
Mly113I	GGCGCC	1 cut(s)	3116
MlyI	GAGTC	3 cut(s)	137, 633, 2291
MmeI	TCCRAC	5 cut(s)	45, 72, 171, 198, 2781
Mox20I	TGGCCA	1 cut(s)	929
Mph1103I	ATGCAT	1 cut(s)	2900
MroI	TCCGGA	1 cut(s)	3164
MroXI	GAANNNNTTC	3 cut(s)	1164, 1290, 1998
MscI	TGGCCA	1 cut(s)	929
MslI	CAYNNNNRTG	2 cut(s)	870, 1403
Msp20I	TGGCCA	1 cut(s)	929
MspA1I	CMGCKG	1 cut(s)	140
MspCI	CTTAAG	1 cut(s)	521
MspI	CCGG	6 cut(s)	168, 891, 1225, 1476, 1870, 3165
MspR9I	CCNGG	5 cut(s)	89, 168, 892, 1458, 1698
Mva1269I	GAATGC	2 cut(s)	521, 2882
MvaI	CCWGG	3 cut(s)	89, 1458, 1698
MvnI	CGCG	1 cut(s)	562
NarI	GGCGCC	1 cut(s)	3116
NciI	CCSGG	2 cut(s)	168, 892
NcoI	CCATGG	3 cut(s)	2819, 3132, 3189
NdeI	CATATG	1 cut(s)	756
NlaIV	GGNNCC	7 cut(s)	172, 556, 1559, 1625, 1799, 2481, 3117
NmeAIII	GCCGAG	1 cut(s)	2662
NmuCI	GTSAC	3 cut(s)	434, 910, 1308
NsiI	ATGCAT	1 cut(s)	2900
NspI	RCATGY	3 cut(s)	295, 1309, 2356
NspV	TTCGAA	2 cut(s)	14, 1240
PaeI	GCATGC	1 cut(s)	2356
PagI	TCATGA	1 cut(s)	2778
PaqCI	CACCTGC	1 cut(s)	13
PciSI	GCTCTTC	1 cut(s)	66
PctI	GAATGC	2 cut(s)	521, 2882
PdmI	GAANNNNTTC	3 cut(s)	1164, 1290, 1998
PfeI	GAWTC	6 cut(s)	663, 802, 1550, 1948, 2449, 2815
PkrI	GCNGC	8 cut(s)	21, 346, 402, 564, 1410, 1587, 2635, 3144
Ple19I	CGATCG	1 cut(s)	2884
PleI	GAGTC	3 cut(s)	137, 633, 2290
PluTI	GGCGCC	1 cut(s)	3119
PpsI	GAGTC	3 cut(s)	137, 633, 2290
Ppu21I	YACGTR	1 cut(s)	872
PpuMI	RGGWCCY	3 cut(s)	877, 1219, 1558
PshBI	ATTAAT	1 cut(s)	1752
PsiI	TTATAA	1 cut(s)	1920
Psp124BI	GAGCTC	1 cut(s)	120
Psp5II	RGGWCCY	3 cut(s)	877, 1219, 1558
Psp6I	CCWGG	3 cut(s)	87, 1456, 1696
PspEI	GGTNACC	1 cut(s)	434
PspFI	CCCAGC	1 cut(s)	545
PspGI	CCWGG	3 cut(s)	87, 1456, 1696
PspN4I	GGNNCC	7 cut(s)	172, 556, 1559, 1625, 1799, 2481, 3117
PspPI	GGNCC	8 cut(s)	749, 877, 1219, 1460, 1558, 2592, 3082, 3129
PspPPI	RGGWCCY	3 cut(s)	877, 1219, 1558
PsrI	GAACNNNNNNTAC	2 cut(s)	3044, 3076
PstI	CTGCAG	1 cut(s)	1768
PstNI	CAGNNNCTG	1 cut(s)	2285
PsuI	RGATCY	3 cut(s)	1351, 2497, 2835
PvuI	CGATCG	1 cut(s)	2884
RsaI	GTAC	5 cut(s)	1072, 1328, 1625, 2421, 2867
RsaNI	GTAC	5 cut(s)	1071, 1327, 1624, 2420, 2866
RseI	CAYNNNNRTG	2 cut(s)	870, 1403
SacI	GAGCTC	1 cut(s)	120
SalI	GTCGAC	1 cut(s)	104
SapI	GCTCTTC	1 cut(s)	66
SatI	GCNGC	8 cut(s)	20, 345, 401, 563, 1409, 1586, 2634, 3143
Sau96I	GGNCC	8 cut(s)	749, 877, 1219, 1460, 1558, 2592, 3082, 3129
ScaI	AGTACT	1 cut(s)	1328
SchI	GAGTC	3 cut(s)	137, 633, 2291
ScrFI	CCNGG	5 cut(s)	89, 168, 892, 1458, 1698
SduI	GDGCHC	4 cut(s)	120, 1834, 2320, 3158
SfaNI	GCATC	7 cut(s)	322, 455, 1660, 1672, 1927, 2104, 2131
SfcI	CTRYAG	2 cut(s)	1330, 1764
SfoI	GGCGCC	1 cut(s)	3117
SfuI	TTCGAA	2 cut(s)	14, 1240
SinI	GGWCC	3 cut(s)	877, 1219, 1558
SmiMI	CAYNNNNRTG	2 cut(s)	870, 1403
SmlI	CTYRAG	2 cut(s)	521, 1803
SmoI	CTYRAG	2 cut(s)	521, 1803
SphI	GCATGC	1 cut(s)	2356
SspDI	GGCGCC	1 cut(s)	3115
SspI	AATATT	2 cut(s)	327, 1566
SspMI	CTAG	7 cut(s)	240, 279, 1554, 1581, 1589, 2754, 3012
SstI	GAGCTC	1 cut(s)	120
StyD4I	CCNGG	5 cut(s)	87, 166, 890, 1456, 1696
StyI	CCWWGG	5 cut(s)	2040, 2311, 2819, 3132, 3189
TaaI	ACNGT	3 cut(s)	1326, 1790, 1981
TaiI	ACGT	3 cut(s)	874, 1384, 2425
TaqI	TCGA	7 cut(s)	14, 105, 120, 1240, 1727, 2341, 3122
TatI	WGTACW	2 cut(s)	1326, 2865
TauI	GCSGC	3 cut(s)	22, 403, 565
TfiI	GAWTC	6 cut(s)	663, 802, 1550, 1948, 2449, 2815
TscAI	CASTG	4 cut(s)	1020, 1768, 1795, 1885
TseFI	GTSAC	3 cut(s)	434, 910, 1308
TseI	GCWGC	5 cut(s)	344, 1408, 1585, 2633, 3142
Tsp45I	GTSAC	3 cut(s)	434, 910, 1308
TspRI	CASTG	4 cut(s)	1020, 1768, 1795, 1885
Vha464I	CTTAAG	1 cut(s)	521
VpaK11BI	GGWCC	3 cut(s)	877, 1219, 1558
VspI	ATTAAT	1 cut(s)	1752
XagI	CCTNNNNNAGG	2 cut(s)	524, 2587
XceI	RCATGY	3 cut(s)	295, 1309, 2356
XcmI	CCANNNNNNNNNTGG	1 cut(s)	712
XmiI	GTMKAC	2 cut(s)	105, 1886
XmnI	GAANNNNTTC	3 cut(s)	1164, 1290, 1998
XspI	CTAG	7 cut(s)	240, 279, 1554, 1581, 1589, 2754, 3012
ZrmI	AGTACT	1 cut(s)	1328
Zsp2I	ATGCAT	1 cut(s)	2900

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.