Rw4G020300

chromatin-remodeling complex ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008488
Physical Location & Seq
Reverse (-)
44132979 .. 44140817
7839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020300.1

Sequence Viewer

Length: 3213 bp
ATGGCGCGAGCCCCGAAGCCCCAGGTCTCTTCCGACGAAGCCCTATCCAATTCCGAGGAGGAGGAGCCGATCAACGAGGAAGAGGACGAGGAGGAGCTCGAGGCCGTCGCTCGGCCCGCCGACTCCGACGACGAGGACGCCGCCGAGGACAACTCCCGCGGCTCCGATGACGAGGCCGCGCCGGTCGAGAACGACGATGACTTTGAAGAAGAGGATGGAGGTAATGAAAGCAATGCTGAAGTCAGCAAACGCGAGAAGGCCAGGCTTAAACGCATGGAACAAATGAAGAAACAAAAGATTCAGGAAATATTGGATGCACAAAATGCAGCTGTGGATGCTGACATGAACAATCGAGGGAAGGGTCGGTTGCAATATCTTCTGCAGCAAACAGAGTTGTTTGCCCATTTTGCCAAAGGTGACTCGTCTGCATCCCTGAAGAAGGTGAAGGGAAGGGGGCGCCATGCTTCAAAAAAGACTGAAGAGGAAGAGGATGAAGAATGCCTAAAAGAGGAAGAAGATGGTTTATCTGGGACCGGAACGACACGGCTATTGACGCAACCTTCCTGTATTCAGGGGAAGATGAGGGATTACCAACTTGCTGGATTAAACTGGCTCATACGCCTCTATGAAAATGGCATAAATGGAATCCTTGCAGATGAGATGGGTCTTGGTAAAACTTTGCAAACCATATCCTTGTTGGGTTACCTGCATGAATTCAGAGGAATAACCGGCCCTCATATGGTTGTGGCTCCAAAGTCAACCCTTGGAAACTGGATGAATGAGATTCGGCGTTTCTGTCCCATTTTGCGTGCTGTGAAGTTTCTTGGTAATCCTGATGAAAGAAAGCATATACGTGAGGAATTGCTGGTTGCTGGGAAGTTTGATGTTTGTGTTACAAGTTTTGAAATGGCCATCAAAGAGAAGTCTTGCTTACGCCGTTTCAGCTGGCGATATATAATCATTGATGAAGCCCATCGAATCAAGAATGAGAATTCACTTCTTTCGAAAACAATGAGGCTGTATAATACCAACTACCGTCTTCTAATCACAGGCACACCGCTTCAGAATAATCTTCATGAACTATGGTCTCTGCTTAACTTTCTTCTGCCAGAGATATTTAGTTCTGCTGAAACTTTTGATGAATGGTTTCAAATATCTGGTGAAAATGACCAGCAGGAGGTTGTTCAACAGCTTCACAAGGTCCTCCGGCCATTCCTTCTCCGAAGGTTGAAGTCAGATGTTGAGAAAGGTTTACCTCCAAAAAAAGAAACAATACTCAAAGTAGGCATGTCACAAATGCAGAAACAGTACTATAAGGCTTTACTGCAGAAAGATCTTGAAGTTGTTAATGCTGGTGGAGAACGTAAGCGGCTTCTGAATATAGCTATGCAGCTTCGTAAATGCTGTAATCATCCATATCTCTTCCAAGGTGCTGAGCCTGGGCCTCCTTATACAACTGGAGACCATCTTATCACAAATGCTGGTAAAATGGTGCTTTTGGACAAGTTACTTCCAAAGTTAAAAGAGCGTGATTCTAGGGTCTTAATATTTTCACAGATGACAAGGCTGCTAGATATTCTCGAAGATTATTTAATGTTTAAAGGATACCTTTATTGTCGGATTGATGGAAATACTGGTGGTGAAGACCGTGATGCTTCCATCGATGCCTTCAATAAGCCTGGAAGTGAGAAATTTGTATTCTTGCTATCAACCAGAGCTGGAGGACTTGGTATCAATCTTGCTACTGCAGATGTCGTTATTCTTTATGATAGTGATTGGAACCCTCAAGTTGATTTGCAAGCACAAGATCGTGCTCACAGGATTGGTCAGAAGAAAGAAGTTCAAGTGTTCCGGTTCTGCACTGAGTATACCATTGAGGAAAAAGTGATTGAGAGGGCTTATAAAAAGCTTGCCCTTGATGCTTTGGTGATTCAACAAGGACGATTAGCTGAGCAAAAAACTGTTAATAAAGATGAGCTACTTCAAATGGTGAGGTTTGGTGCTGAAATGGTTTTTAGTTCCAAGGATAGCACAATTACCGATGAGGACATTGATAGAATCATCGCGAAAGGAGAAGAGGCAACTGCTGAGCTTGATGCAAAGATGAAGAAATTTACTGAGGATGCGATTAAATTTAAGATGGATGATACTGCTGAATTGTATGACTTTGATGATGAGAAGGATGAGAAGTTTGACTTCAAGAAGATTGTTAGTGAAAACTGGATTGAACCACCAAAAAGAGAGCGGAAGCGCAATTACTCAGAATCAGAATACTTTAAGCAAACAATGCGCCAAGGTGCTCCAGCAAAACCCAAAGAGCCTCGAATTCCACGCATGCCTCAATTACATGATTTTCAATTTTTCAACACACAAAGGCTGAGTGAGCTGTATGAAAAGGAAGTACGCTACCTCATGCAAAATCATCAGAAGAATCAGTTGAAGGACACGACTATAGAAGTGGATGAACCAGAAGAAGTTGGAGATCCATTGACTGCTGAAGAGCTGGAAGAAAAGGAACGTCTATTAGAAGAGGGATTCTCTTCGTGGAGTAGAAGAGATTTCAATACCTTCATCAGGGCCTGTGAGAAGTATGGGCGAAGTGACATACAAAGCATTGTTGCTGAGATGGACGGGAAAACAGAGGAGGAAGTCGAAAGATATGCTAAGGTTTTCAAAGAGAGATACAAGGAATTAAATGATTATGATAGGATCATTAAGAACATTGAAAGAGGAGAGGCTAGAATCTCTCGTAAGGATGAGATAATGAAAGCTATTGGGAAGAAGTTGGATCGTTACAAGAATCCATGGCTAGAACTGAAGATCCTTTATGGTCAGAACAAGGGAAAGTTGTACAATGAAGAATGTGACCGTTTCATGATATGCATGGTTCACAAGCTTGGGTATGGAAACTGGGATGAGTTGAAAGCAGCATTTCGCACGTCACCTTTGTTTCGTTTTGATTGGTTTGTGAAGTCGCGCACAACTCAGGAACTTGCTAGGAGGTGTGATACATTAATTCGATTGGTGGAGAAAGAGAACCAAGAAGTTGATGAGAGGGAGAGACAAGCTCGCAAAGAGAAGAAGCTTGCTAAGAACATGACACCATCAAAGCGTGGTACGGGGAGGCAGCCAACTGAGAGCCCCTCTTCTTTGAAGAAGAGGAAGCAATTAACTATGGACGATTATGTTAGCACGGGAAAGAGGAGGAGATGA

Protein Analysis

1070

Amino Acids

124.14

Weight (kDa)

5.7

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 193 - 353 6.8e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 197 - 474 8.1e-101 SNF2-related domain
Helicase_C PF00271 496 - 609 9.2e-25 Helicase conserved C-terminal domain
HAND PF09110 757 - 844 9.2e-07 HAND
SLIDE PF09111 902 - 1012 1.9e-42 SLIDE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1902
Acc36I ACCTGC 1 cut(s) 714
AccB1I GGYRCC 1 cut(s) 456
AccBSI CCGCTC 1 cut(s) 2245
AccI GTMKAC 1 cut(s) 1868
AccII CGCG 6 cut(s) 7, 159, 179, 252, 2066, 2977
AciI CCGC 8 cut(s) 117, 141, 157, 159, 177, 1058, 1369, 2245
AclWI GGATC 4 cut(s) 2477, 2717, 2796, 2815
AcoI YGGCCR 2 cut(s) 909, 1208
AcsI RAATTY 6 cut(s) 713, 991, 1691, 2111, 2132, 2325
AcuI CTGAAG 6 cut(s) 258, 455, 498, 1046, 2517, 2837
AcyI GRCGYC 2 cut(s) 138, 457
AfaI GTAC 4 cut(s) 1310, 2403, 2852, 3118
AfiI CCNNNNNNNGG 6 cut(s) 111, 439, 508, 739, 1177, 2574
AjiI CACGTC 1 cut(s) 2940
AjnI CCWGG 4 cut(s) 21, 260, 1438, 1678
AloI GAACNNNNNNTCC 2 cut(s) 2805, 2837
Alw21I GWGCWC 3 cut(s) 99, 1816, 2302
Alw26I GTCTC 4 cut(s) 31, 1092, 1455, 3055
AlwI GGATC 4 cut(s) 2477, 2717, 2796, 2815
AlwNI CAGNNNCTG 1 cut(s) 2580
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 9 cut(s) 102, 113, 174, 258, 730, 909, 1208, 1442, 2577
ApeKI GCWGC 6 cut(s) 326, 382, 1390, 1567, 2927, 3127
ApoI RAATTY 6 cut(s) 713, 991, 1691, 2111, 2132, 2325
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AseI ATTAAT 1 cut(s) 3014
Asp700I GAANNNNTTC 1 cut(s) 1146
AspLEI GCGC 6 cut(s) 7, 181, 459, 2253, 2292, 2979
AspS9I GGNCC 6 cut(s) 114, 531, 731, 1201, 1442, 2577
AsuHPI GGTGA 7 cut(s) 428, 454, 1172, 1652, 1939, 2002, 2934
AsuII TTCGAA 1 cut(s) 1004
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 2 cut(s) 531, 1201
BalI TGGCCA 1 cut(s) 911
BanI GGYRCC 1 cut(s) 456
BanII GRGCYC 3 cut(s) 13, 99, 3143
BarI GAAGNNNNNNTAC 2 cut(s) 2240, 2272
BbsI GAAGAC 2 cut(s) 1031, 1650
Bbv12I GWGCWC 3 cut(s) 99, 1816, 2302
BbvI GCAGC 6 cut(s) 338, 394, 1402, 1554, 2939, 3139
BceAI ACGGC 3 cut(s) 89, 560, 921
BcgI CGANNNNNNTGC 2 cut(s) 2642, 2676
BciT130I CCWGG 4 cut(s) 23, 262, 1440, 1680
BciVI GTATCC 1 cut(s) 1598
BcoDI GTCTC 4 cut(s) 31, 1092, 1455, 3055
BfaI CTAG 5 cut(s) 1536, 1571, 2739, 2810, 2997
BfmI CTRYAG 4 cut(s) 380, 1325, 1746, 2451
BfoI RGCGCY 1 cut(s) 460
BfuAI ACCTGC 1 cut(s) 714
BfuI GTATCC 1 cut(s) 1598
BglII AGATCT 1 cut(s) 1333
BlpI GCTNAGC 3 cut(s) 1434, 1950, 2088
BmcAI AGTACT 1 cut(s) 1310
Bme1390I CCNGG 4 cut(s) 23, 262, 1440, 1680
Bme18I GGWCC 2 cut(s) 531, 1201
BmeT110I CYCGRG 1 cut(s) 98
BmgBI CACGTC 1 cut(s) 2940
BmgT120I GGNCC 6 cut(s) 114, 531, 731, 1201, 1442, 2577
BmiI GGNNCC 6 cut(s) 66, 163, 458, 532, 750, 1781
BmrFI CCNGG 4 cut(s) 23, 262, 1440, 1680
BmrI ACTGGG 1 cut(s) 2920
BmsI GCATC 8 cut(s) 304, 325, 437, 1642, 1654, 1909, 2086, 2113
BmuI ACTGGG 1 cut(s) 2920
BpiI GAAGAC 2 cut(s) 1031, 1650
BplI GAGNNNNNCTC 8 cut(s) 137, 169, 2522, 2554, 3052, 3084, 3128, 3160
BpmI CTGGAG 3 cut(s) 1479, 1740, 2286
Bpu10I CCTNAGC 1 cut(s) 2664
Bpu1102I GCTNAGC 3 cut(s) 1434, 1950, 2088
Bpu14I TTCGAA 1 cut(s) 1004
BpuEI CTTGAG 1 cut(s) 1770
Bsa29I ATCGAT 1 cut(s) 1662
BsaAI YACGTR 1 cut(s) 854
BsaHI GRCGYC 2 cut(s) 138, 457
BsaI GGTCTC 3 cut(s) 31, 1092, 1455
BsaWI WCCGGW 2 cut(s) 533, 1851
Bsc4I CCNNNNNNNGG 6 cut(s) 111, 439, 508, 739, 1177, 2574
Bse118I RCCGGY 2 cut(s) 181, 728
Bse1I ACTGG 6 cut(s) 614, 776, 1462, 1639, 2225, 2915
Bse3DI GCAATG 1 cut(s) 238
BseBI CCWGG 4 cut(s) 23, 262, 1440, 1680
BseCI ATCGAT 1 cut(s) 1662
BseLI CCNNNNNNNGG 6 cut(s) 111, 439, 508, 739, 1177, 2574
BseMI GCAATG 1 cut(s) 238
BseNI ACTGG 6 cut(s) 614, 776, 1462, 1639, 2225, 2915
BseRI GAGGAG 7 cut(s) 71, 74, 77, 104, 107, 2657, 2745
BseXI GCAGC 6 cut(s) 338, 394, 1402, 1554, 2939, 3139
BseYI CCCAGC 1 cut(s) 872
BsgI GTGCAG 1 cut(s) 1843
Bsh1236I CGCG 6 cut(s) 7, 159, 179, 252, 2066, 2977
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 9 cut(s) 104, 115, 176, 260, 732, 911, 1210, 1444, 2579
BshNI GGYRCC 1 cut(s) 456
BshVI ATCGAT 1 cut(s) 1662
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 3 cut(s) 99, 1816, 2302
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 5 cut(s) 182, 534, 729, 1207, 1852
BslFI GGGAC 2 cut(s) 544, 783
BslI CCNNNNNNNGG 6 cut(s) 111, 439, 508, 739, 1177, 2574
BsmAI GTCTC 4 cut(s) 31, 1092, 1455, 3055
BsmFI GGGAC 2 cut(s) 544, 783
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 9 cut(s) 104, 115, 176, 260, 732, 911, 1210, 1444, 2579
Bso31I GGTCTC 3 cut(s) 31, 1092, 1455
BsoBI CYCGRG 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 1004
Bsp1286I GDGCHC 5 cut(s) 13, 99, 1816, 2302, 3143
Bsp1407I TGTACA 1 cut(s) 2850
Bsp143I GATC 7 cut(s) 69, 1333, 1807, 2482, 2709, 2788, 2820
Bsp1720I GCTNAGC 3 cut(s) 1434, 1950, 2088
Bsp19I CCATGG 1 cut(s) 2804
Bsp68I TCGCGA 1 cut(s) 2066
BspACI CCGC 8 cut(s) 117, 141, 157, 159, 177, 1058, 1369, 2245
BspANI GGCC 9 cut(s) 104, 115, 176, 260, 732, 911, 1210, 1444, 2579
BspDI ATCGAT 1 cut(s) 1662
BspFNI CGCG 6 cut(s) 7, 159, 179, 252, 2066, 2977
BspHI TCATGA 2 cut(s) 1075, 2874
BspLI GGNNCC 6 cut(s) 66, 163, 458, 532, 750, 1781
BspMAI CTGCAG 3 cut(s) 384, 1329, 1750
BspMI ACCTGC 1 cut(s) 714
BspPI GGATC 4 cut(s) 2477, 2717, 2796, 2815
BspQI GCTCTTC 1 cut(s) 2493
BspT104I TTCGAA 1 cut(s) 1004
BspT107I GGYRCC 1 cut(s) 456
BspTNI GGTCTC 3 cut(s) 31, 1092, 1455
BsrBI CCGCTC 1 cut(s) 2245
BsrDI GCAATG 1 cut(s) 238
BsrFI RCCGGY 2 cut(s) 181, 728
BsrGI TGTACA 1 cut(s) 2850
BsrI ACTGG 6 cut(s) 614, 776, 1462, 1639, 2225, 2915
BssAI RCCGGY 2 cut(s) 181, 728
BssMI GATC 7 cut(s) 69, 1333, 1807, 2482, 2709, 2788, 2820
BssNAI GTATAC 1 cut(s) 1869
BssNI GRCGYC 2 cut(s) 138, 457
BssT1I CCWWGG 5 cut(s) 763, 1426, 2022, 2293, 2804
Bst1107I GTATAC 1 cut(s) 1869
Bst2UI CCWGG 4 cut(s) 23, 262, 1440, 1680
Bst4CI ACNGT 5 cut(s) 1037, 1308, 1649, 1963, 2870
BstACI GRCGYC 2 cut(s) 138, 457
BstAPI GCANNNNNTGC 2 cut(s) 323, 2287
BstAUI TGTACA 1 cut(s) 2850
BstBAI YACGTR 1 cut(s) 854
BstBI TTCGAA 1 cut(s) 1004
BstC8I GCNNGC 9 cut(s) 9, 117, 810, 947, 1800, 1911, 2336, 3070, 3087
BstDSI CCRYGG 2 cut(s) 157, 2804
BstEII GGTNACC 1 cut(s) 701
BstENI CCTNNNNNAGG 3 cut(s) 437, 506, 2572
BstFNI CGCG 6 cut(s) 7, 159, 179, 252, 2066, 2977
BstH2I RGCGCY 1 cut(s) 460
BstHHI GCGC 6 cut(s) 7, 181, 459, 2253, 2292, 2979
BstKTI GATC 7 cut(s) 72, 1336, 1810, 2485, 2712, 2791, 2823
BstMAI GTCTC 4 cut(s) 31, 1092, 1455, 3055
BstMBI GATC 7 cut(s) 69, 1333, 1807, 2482, 2709, 2788, 2820
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 9 cut(s) 116, 323, 335, 407, 553, 942, 1919, 2287, 2383
BstNI CCWGG 4 cut(s) 23, 262, 1440, 1680
BstNSI RCATGY 2 cut(s) 1291, 2338
BstPI GGTNACC 1 cut(s) 701
BstSCI CCNGG 4 cut(s) 21, 260, 1438, 1678
BstSFI CTRYAG 4 cut(s) 380, 1325, 1746, 2451
BstUI CGCG 6 cut(s) 7, 159, 179, 252, 2066, 2977
BstV1I GCAGC 6 cut(s) 338, 394, 1402, 1554, 2939, 3139
BstV2I GAAGAC 2 cut(s) 1031, 1650
BstX2I RGATCY 3 cut(s) 1333, 2482, 2820
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 3 cut(s) 1333, 2482, 2820
BstZ17I GTATAC 1 cut(s) 1869
Bsu15I ATCGAT 1 cut(s) 1662
BsuI GTATCC 1 cut(s) 1598
BsuRI GGCC 9 cut(s) 104, 115, 176, 260, 732, 911, 1210, 1444, 2579
BsuTUI ATCGAT 1 cut(s) 1662
BtgI CCRYGG 2 cut(s) 157, 2804
BtgZI GCGATG 1 cut(s) 2047
BtrI CACGTC 1 cut(s) 2940
BtsIMutI CAGTG 1 cut(s) 1860
BtuMI TCGCGA 1 cut(s) 2066
BveI ACCTGC 1 cut(s) 714
Cac8I GCNNGC 9 cut(s) 9, 117, 810, 947, 1800, 1911, 2336, 3070, 3087
CaiI CAGNNNCTG 1 cut(s) 2580
CciI TCATGA 2 cut(s) 1075, 2874
CfoI GCGC 6 cut(s) 7, 181, 459, 2253, 2292, 2979
Cfr10I RCCGGY 2 cut(s) 181, 728
Cfr13I GGNCC 6 cut(s) 114, 531, 731, 1201, 1442, 2577
Cfr42I CCGCGG 1 cut(s) 160
ClaI ATCGAT 1 cut(s) 1662
CseI GACGC 2 cut(s) 146, 562
Csp6I GTAC 4 cut(s) 1309, 2402, 2851, 3117
CviQI GTAC 4 cut(s) 1309, 2402, 2851, 3117
DinI GGCGCC 1 cut(s) 458
DpnI GATC 7 cut(s) 71, 1335, 1809, 2484, 2711, 2790, 2822
DpnII GATC 7 cut(s) 69, 1333, 1807, 2482, 2709, 2788, 2820
DraI TTTAAA 1 cut(s) 1600
EaeI YGGCCR 2 cut(s) 909, 1208
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 5 cut(s) 763, 1426, 2022, 2293, 2804
Eco24I GRGCYC 3 cut(s) 13, 99, 3143
Eco31I GGTCTC 3 cut(s) 31, 1092, 1455
Eco47I GGWCC 2 cut(s) 531, 1201
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 6 cut(s) 258, 455, 498, 1046, 2517, 2837
Eco88I CYCGRG 1 cut(s) 98
Eco91I GGTNACC 1 cut(s) 701
EcoICRI GAGCTC 1 cut(s) 97
EcoNI CCTNNNNNAGG 3 cut(s) 437, 506, 2572
EcoO109I RGGNCCY 2 cut(s) 1201, 2577
EcoO65I GGTNACC 1 cut(s) 701
EcoRI GAATTC 3 cut(s) 713, 991, 2325
EcoRII CCWGG 4 cut(s) 21, 260, 1438, 1678
EcoT14I CCWWGG 5 cut(s) 763, 1426, 2022, 2293, 2804
EcoT22I ATGCAT 1 cut(s) 2885
EcoT38I GRGCYC 3 cut(s) 13, 99, 3143
EgeI GGCGCC 1 cut(s) 458
EheI GGCGCC 1 cut(s) 458
ErhI CCWWGG 5 cut(s) 763, 1426, 2022, 2293, 2804
FalI AAGNNNNNCTT 6 cut(s) 914, 946, 1593, 1625, 2180, 2212
FaqI GGGAC 2 cut(s) 544, 783
FauI CCCGC 2 cut(s) 124, 164
FauNDI CATATG 1 cut(s) 738
FblI GTMKAC 1 cut(s) 1868
FriOI GRGCYC 3 cut(s) 13, 99, 3143
FspBI CTAG 5 cut(s) 1536, 1571, 2739, 2810, 2997
GlaI GCGC 6 cut(s) 6, 180, 458, 2252, 2291, 2978
GsaI CCCAGC 1 cut(s) 876
GsuI CTGGAG 3 cut(s) 1479, 1740, 2286
HaeII RGCGCY 1 cut(s) 460
HaeIII GGCC 9 cut(s) 104, 115, 176, 260, 732, 911, 1210, 1444, 2579
HapII CCGG 5 cut(s) 182, 534, 729, 1207, 1852
HgaI GACGC 2 cut(s) 146, 562
HhaI GCGC 6 cut(s) 7, 181, 459, 2253, 2292, 2979
Hin1I GRCGYC 2 cut(s) 138, 457
Hin6I GCGC 6 cut(s) 5, 179, 457, 2251, 2290, 2977
HinP1I GCGC 6 cut(s) 5, 179, 457, 2251, 2290, 2977
HincII GTYRAC 1 cut(s) 759
HindII GTYRAC 1 cut(s) 759
HindIII AAGCTT 3 cut(s) 1907, 2894, 3083
HpaII CCGG 5 cut(s) 182, 534, 729, 1207, 1852
HphI GGTGA 7 cut(s) 428, 454, 1172, 1652, 1939, 2002, 2934
Hpy166II GTNNAC 4 cut(s) 759, 1253, 1869, 2890
Hpy8I GTNNAC 4 cut(s) 759, 1253, 1869, 2890
Hpy99I CGWCG 5 cut(s) 38, 110, 131, 134, 197
HpyCH4III ACNGT 5 cut(s) 1037, 1308, 1649, 1963, 2870
HpyCH4IV ACGT 4 cut(s) 853, 1363, 2518, 2939
HpyF10VI GCNNNNNNNGC 9 cut(s) 116, 323, 335, 407, 553, 942, 1919, 2287, 2383
HpySE526I ACGT 4 cut(s) 853, 1363, 2518, 2939
Hsp92I GRCGYC 2 cut(s) 138, 457
HspAI GCGC 6 cut(s) 5, 179, 457, 2251, 2290, 2977
KasI GGCGCC 1 cut(s) 456
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 7 cut(s) 69, 1333, 1807, 2482, 2709, 2788, 2820
LguI GCTCTTC 1 cut(s) 2493
LmnI GCTCC 5 cut(s) 64, 94, 167, 754, 2305
Lsp1109I GCAGC 6 cut(s) 338, 394, 1402, 1554, 2939, 3139
LweI GCATC 8 cut(s) 304, 325, 437, 1642, 1654, 1909, 2086, 2113
MaeI CTAG 5 cut(s) 1536, 1571, 2739, 2810, 2997
MaeII ACGT 4 cut(s) 853, 1363, 2518, 2939
MaeIII GTNAC 9 cut(s) 416, 701, 892, 1290, 1506, 2600, 2792, 2864, 2940
MalI GATC 7 cut(s) 71, 1335, 1809, 2484, 2711, 2790, 2822
MbiI CCGCTC 1 cut(s) 2245
MboI GATC 7 cut(s) 69, 1333, 1807, 2482, 2709, 2788, 2820
MflI RGATCY 3 cut(s) 1333, 2482, 2820
MhlI GDGCHC 5 cut(s) 13, 99, 1816, 2302, 3143
MlsI TGGCCA 1 cut(s) 911
MluNI TGGCCA 1 cut(s) 911
Mly113I GGCGCC 1 cut(s) 457
MlyI GAGTC 2 cut(s) 116, 413
MmeI TCCRAC 5 cut(s) 57, 150, 1598, 2458, 2766
Mox20I TGGCCA 1 cut(s) 911
Mph1103I ATGCAT 1 cut(s) 2885
MroXI GAANNNNTTC 1 cut(s) 1146
MscI TGGCCA 1 cut(s) 911
MslI CAYNNNNRTG 1 cut(s) 852
Msp20I TGGCCA 1 cut(s) 911
MspA1I CMGCKG 3 cut(s) 159, 329, 945
MspI CCGG 5 cut(s) 182, 534, 729, 1207, 1852
MspR9I CCNGG 4 cut(s) 23, 262, 1440, 1680
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 4 cut(s) 23, 262, 1440, 1680
MvnI CGCG 6 cut(s) 7, 159, 179, 252, 2066, 2977
MwoI GCNNNNNNNGC 9 cut(s) 116, 323, 335, 407, 553, 942, 1919, 2287, 2383
NarI GGCGCC 1 cut(s) 457
NcoI CCATGG 1 cut(s) 2804
NdeI CATATG 1 cut(s) 738
NdeII GATC 7 cut(s) 69, 1333, 1807, 2482, 2709, 2788, 2820
NlaIV GGNNCC 6 cut(s) 66, 163, 458, 532, 750, 1781
NmeAIII GCCGAG 2 cut(s) 91, 169
NmuCI GTSAC 5 cut(s) 416, 1290, 2600, 2864, 2940
NruI TCGCGA 1 cut(s) 2066
NsiI ATGCAT 1 cut(s) 2885
NspI RCATGY 2 cut(s) 1291, 2338
NspV TTCGAA 1 cut(s) 1004
PaeI GCATGC 1 cut(s) 2338
PaeR7I CTCGAG 1 cut(s) 98
PagI TCATGA 2 cut(s) 1075, 2874
PciSI GCTCTTC 1 cut(s) 2493
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 1 cut(s) 503
PdmI GAANNNNTTC 1 cut(s) 1146
PleI GAGTC 2 cut(s) 116, 413
PluTI GGCGCC 1 cut(s) 460
PpsI GAGTC 2 cut(s) 116, 413
Ppu21I YACGTR 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 1201
PshBI ATTAAT 1 cut(s) 3014
PsiI TTATAA 1 cut(s) 1902
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 1201
Psp6I CCWGG 4 cut(s) 21, 260, 1438, 1678
PspEI GGTNACC 1 cut(s) 701
PspFI CCCAGC 1 cut(s) 872
PspGI CCWGG 4 cut(s) 21, 260, 1438, 1678
PspN4I GGNNCC 6 cut(s) 66, 163, 458, 532, 750, 1781
PspPI GGNCC 6 cut(s) 114, 531, 731, 1201, 1442, 2577
PspPPI RGGWCCY 1 cut(s) 1201
PspXI VCTCGAGB 1 cut(s) 98
PstI CTGCAG 3 cut(s) 384, 1329, 1750
PstNI CAGNNNCTG 1 cut(s) 2580
PsuI RGATCY 3 cut(s) 1333, 2482, 2820
PvuII CAGCTG 2 cut(s) 329, 945
RruI TCGCGA 1 cut(s) 2066
RsaI GTAC 4 cut(s) 1310, 2403, 2852, 3118
RsaNI GTAC 4 cut(s) 1309, 2402, 2851, 3117
RseI CAYNNNNRTG 1 cut(s) 852
SacI GAGCTC 1 cut(s) 99
SacII CCGCGG 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 2493
Sau3AI GATC 7 cut(s) 69, 1333, 1807, 2482, 2709, 2788, 2820
Sau96I GGNCC 6 cut(s) 114, 531, 731, 1201, 1442, 2577
ScaI AGTACT 1 cut(s) 1310
SchI GAGTC 2 cut(s) 116, 413
ScrFI CCNGG 4 cut(s) 23, 262, 1440, 1680
SduI GDGCHC 5 cut(s) 13, 99, 1816, 2302, 3143
SfaNI GCATC 8 cut(s) 304, 325, 437, 1642, 1654, 1909, 2086, 2113
SfcI CTRYAG 4 cut(s) 380, 1325, 1746, 2451
SfoI GGCGCC 1 cut(s) 458
Sfr274I CTCGAG 1 cut(s) 98
Sfr303I CCGCGG 1 cut(s) 160
SfuI TTCGAA 1 cut(s) 1004
SgrBI CCGCGG 1 cut(s) 160
SinI GGWCC 2 cut(s) 531, 1201
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 2 cut(s) 98, 1785
SmoI CTYRAG 2 cut(s) 98, 1785
SphI GCATGC 1 cut(s) 2338
SsiI CCGC 8 cut(s) 117, 141, 157, 159, 177, 1058, 1369, 2245
SspDI GGCGCC 1 cut(s) 456
SspI AATATT 2 cut(s) 309, 1548
SspMI CTAG 5 cut(s) 1536, 1571, 2739, 2810, 2997
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 4 cut(s) 21, 260, 1438, 1678
StyI CCWWGG 5 cut(s) 763, 1426, 2022, 2293, 2804
TaaI ACNGT 5 cut(s) 1037, 1308, 1649, 1963, 2870
TaiI ACGT 4 cut(s) 856, 1366, 2521, 2942
TatI WGTACW 2 cut(s) 1308, 2850
TauI GCSGC 4 cut(s) 143, 162, 179, 1372
TscAI CASTG 1 cut(s) 1867
TseFI GTSAC 5 cut(s) 416, 1290, 2600, 2864, 2940
TseI GCWGC 6 cut(s) 326, 382, 1390, 1567, 2927, 3127
Tsp45I GTSAC 5 cut(s) 416, 1290, 2600, 2864, 2940
TspRI CASTG 1 cut(s) 1867
VpaK11BI GGWCC 2 cut(s) 531, 1201
VspI ATTAAT 1 cut(s) 3014
XagI CCTNNNNNAGG 3 cut(s) 437, 506, 2572
XapI RAATTY 6 cut(s) 713, 991, 1691, 2111, 2132, 2325
XceI RCATGY 2 cut(s) 1291, 2338
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XhoI CTCGAG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 1868
XmnI GAANNNNTTC 1 cut(s) 1146
XspI CTAG 5 cut(s) 1536, 1571, 2739, 2810, 2997
ZrmI AGTACT 1 cut(s) 1310
Zsp2I ATGCAT 1 cut(s) 2885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.