Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008488

Physical Location & Seq

Genomic Coordinates Reverse (-)

14146364 .. 14153268

Gene Structure 6905 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1172500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3237 bp

ATGGCGAGAGCTTCGAAGCCTCAGGTTTCGTCCGACGAAGCCCTATCCAACGGCTCCAATTCGTCCGACGAAGAGCCGATCAACGACCAGGAGGAGGACGAGGTCGATGAGGAGGAGCTCGAAGCCGTGGCTCGCCCCGCTGACTCCGACGACGACGAGGTCGCCGCCGACGAGAACTCCCCTGGCTCCGACGATGACGTGCCGGTCGATGAGAACGATGATGGTTTGGATGGAGAAGAGGATGATGAAGCTAATTTAAGTAATGCTGAAATTGGAAAACGTGAGAAGGCGAGGCTCAGAGACATGCAAAATATGAAGAGACAAAAGGTTCAGGAAATATTGGATGCACAAAATGCAGCCATTGAGGCTGACATGAACAATAAGGGGAAGGGACGGTTGAAGTATCTTCTGCAGCAAACAGAGTTATTTGCCCATTTTGCTAAAGGTGACCCATCTGCATCTCAGAAGAAAGTGAAGGGAAAAGGTCGCCATGCTTCCAAAATAACTGAAGAGGAAGAGGATGAAGAATGCCTTAAGGAGGAAGAAGATGGTTTGGCTGGGGCTGGAACCACACGGCTTTTGACACAACCTTCTTGTATTCAGGGGAAGATGAGGGATTACCAACTGGCTGGATTGAACTGGCTCATACGACTCTATGAAAATGGTATAAATGGAATCCTTGCAGATGAAATGGGGCTTGGTAAAACATTGCAAACCATCTCCTTATTGGGATACCTGCATGAGTTTAGAGGAATAACTGGCCCTCATATGGTAGTTGCTCCAAAGTCTACTCTTGGAAACTGGATGAATGAGATTCGGCGTTTCTGCCCCATCTTGCGTGCTGTGAAGTTTCTTGGTAATCCTGATGAAAGAAAGCATATACGTGAGGACCTGCTGGTTGCCGGGAATTTTGATGTATGTGTTACAAGTTTTGAAATGGCCATCAAAGAGAAGACCTGCTTACGCCGTTTTAGCTGGCGTTATATAATAATTGATGAAGCTCATCGTATCAAGAATGAGAATTCACTGCTTTCAAAAACAATGAGGCTGTATAATACTAACTTCCGCCTCCTAATTACGGGTACACCACTTCAGAATAATCTTCACGAACTATGGTCTCTGCTTAACTTTCTCCTGCCAGAGATATTTAGTTCTGCTGAAACTTTTGATGAATGGTTTCAAATATCTGGTGAGAACGACCAGCAAGAGGTTGTTCAACAGCTTCACAAGGTCCTCCGGCCATTTCTTCTTAGAAGGTTGAAGTCAGATGTTGAGAAAGGTTTGCCTCCGAAAAAAGAAACAATACTCAAAGTAGGCATGTCACAAATGCAGAAACAGTACTACAGGGCTTTATTACAAAAAGATCTTGAAGTTGTTAATGCTGGTGGAGAACGTAAGCGCCTTCTGAACATAGCAATGCAGCTTCGTAAGTGCTGTAATCATCCTTATCTTTTTCAAGGTGCTGAGCCCGGGCCTCCTTATACGACTGGAGACCATCTCATCACAAATGCTGGTAAAATGGTTCTTTTGGATAAGTTGCTTCCAAAGTTAAAGGAGCGTGATTCTAGGGTCCTAATATTTTCACAGATGACTAGGCTGCTAGATATCCTGGAAGATTATTTAATGTTACGGGGGTACCTGTATTGTCGGATTGATGGCAATACTGGTGGAGAAGATCGTGATGCTTCTATTGATGCCTTCAACAAGCCTGGAAGTGAGAAATTTGTATTTTTGCTATCAACCAGAGCTGGAGGACTTGGTATTAATCTTGCCACTGCAGATGTTGTCATCCTTTATGACAGTGATTGGAACCCTCAAGTTGATTTGCAAGCACAAGATCGTGCTCACAGGATTGGTCAAAAGAAAGAAGTTCAAGTGTTCCGGTTCTGCACCGAGTATACTATTGAAGAAAAAGTTATTGAGAGGGCTTATAAAAAGCTTGCTCTTGATGCTTTGGTGATTCAACAAGGGCGCTTAGCAGAGCAAAAAACTGTTAATAAAGATGAGTTACTTCAAATGGTGAGGTTTGGTGCTGAAATGGTCTTCAGTTCCAAGGATAGCACCATTACAGATGAGGACATAGATAGGATTATTGCTAAAGGAGAAGAGACAACTGCTGAGCTTGATGCAAAGATGAAGAAATTTACTGAAGATGCTATCAAATTTAAGATGGATGATACTGCTGAATTGTATGACTTTGATGATGAAAAGGATGACAAGCTTGATTTCAAGAAAATTGTTAGCGATAACTGGATTGAACCACCAAAAAGAGAGCGGAAGCGCAATTACTCAGAGTCCGAATACTTTAAGCAAACAATGCGCCAAGGTGCTCCAGCAAAACCGAAAGAGCCTCGAATTCCACGCATGCCTCAATTACATGATTTCCAATTTTTCAACACACAGAGGTTGAATGAGCTGTATGAGAAGGAAGTACGGTTCCTCATGCAAACTCATCAGAAGAATCAGTTGAAAGATACAATAGAGGTGGAGGAACCAGAAGTTGGAGATCCTTTGACTGCTGAGGAGGTGGAAGAAAAGGAAAGCCTGTTAGAAGAGGGTTTCTCTACTTGGAGTAGAAGAGATTTTAATACCTTCATTAGGGGCTGTGAGAAGTATGGCCGTAATGACATAAAAAGCATTGCTGCTGAGATGGAAGGGAAAACAGAGGAGGAAGTTGAAAGATACGCAAAGGTTTTTAAAGAAAGATACAAGGAGTTAAATGATTATGACAGGATCATTAAGAACATTGAAAGAGGTGAGGCCAGAATTTCCCGCAAAGATGAGATCATGAAAGCCATTGGGAAGAAGTTGGATCGTTACAAGAATCCATGGCTGGAATTGAAAATCCAGTATGGTCAAAACAAGGGGAAGTTGTACAATGAAGAATGCGATCGTTTCATGATATGCATGGTTCACAAACTTGGGTATGGGAATTGGGACGAATTGAAGGCAGAATTCCGCACATCACCCTTGTTTCGTTTTGATTGGTTTGTGAAGTCTCGCACAACTCAGGAACTTGCTAGGAGGTGTGATACATTAATTAGATTGGTGGAGAAAGAGAACCAAGAATACGATGAGAGGGAGAGACAGGCCCGCAAAGAGAAGAAGCTTGCTAAGAGCATGACACCATCAAAGCGGGCCTTGGGTAGACAGCCAACTGAGAGCCCAAATACTGGAAAGAAGAGGAAGCAATTAACCATGGATGATTATGTCAGCTCGGGAAAAAGGAGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006323 GO:0006325 GO:0006333 GO:0006996 GO:0007275 GO:0008150 GO:0009553 GO:0009561 GO:0009987 GO:0016043 GO:0016584 GO:0022607 GO:0031497 GO:0032501 GO:0032502 GO:0034728 GO:0040034 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044464 GO:0048229 GO:0048506 GO:0048509 GO:0048510 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051276 GO:0065007 GO:0071103 GO:0071824 GO:0071840 GO:0080134 GO:0080135 GO:1900034 GO:1900036

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DBINO HAND Helicase_C SLIDE SNF2_N

Protein Analysis

1079

Amino Acids

124.68

Weight (kDa)

5.52

Isoelectric Point (pI)

41.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	203 - 363	3.9e-09	Type III restriction enzyme, res subunit
SNF2-rel_dom	PF00176	207 - 484	4.9e-99	SNF2-related domain
Helicase_C	PF00271	506 - 619	9.4e-25	Helicase conserved C-terminal domain
HAND	PF09110	767 - 851	1.7e-06	HAND
SLIDE	PF09111	910 - 1020	1.7e-43	SLIDE

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06400 AT3G06400 AT3G06400 AT5G18620 AT5G18620
fragaria_vesca	FvH4_4g17400 FvH4_4g17400
malus_domestica	MD13G1172500.v1.1 MD16G1173900.v1.1
prunus_persica	Prupe.1G140700_v2.0.a1 Prupe.1G140700_v2.0.a1 Prupe.1G140700_v2.0.a1
pyrus_communis	pycom13g14810
rosa_chinensis	RchiOBHm_Chr4g0421321
rosa_laevigata	RLG00000007697
rosa_multiflora	Rmu_sc0015713.1_g000006
rosa_roxburghii	Rroxscaffold_5G00363560
rosa_rugosa	Rorug04G0168900
rosa_samantha	Rh4AG230900 Rh4BG233900 Rh4CG245300 Rh4DG229500
rosa_wichuraiana	Rw4G020300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1932
AasI	GACNNNNNNGTC	2 cut(s)	158, 203
Acc36I	ACCTGC	3 cut(s)	744, 900, 965
Acc65I	GGTACC	1 cut(s)	1635
AccB1I	GGYRCC	1 cut(s)	1635
AccB7I	CCANNNNNTGG	2 cut(s)	2501, 3173
AccBSI	CCGCTC	1 cut(s)	2275
AccI	GTMKAC	3 cut(s)	788, 1898, 3148
AciI	CCGC	8 cut(s)	138, 165, 1066, 2275, 2773, 2959, 3094, 3136
AclWI	GGATC	3 cut(s)	2501, 2741, 2820
AcoI	YGGCCR	3 cut(s)	939, 1238, 2617
AcsI	RAATTY	8 cut(s)	907, 1021, 1721, 2141, 2162, 2355, 2766, 2954
AcuI	CTGAAG	4 cut(s)	528, 1076, 2029, 2169
AfaI	GTAC	5 cut(s)	1084, 1340, 1637, 2433, 2876
AfiI	CCNNNNNNNGG	8 cut(s)	94, 538, 769, 1078, 1207, 2501, 2598, 3173
AflII	CTTAAG	1 cut(s)	533
AjiI	CACGTC	1 cut(s)	199
AjnI	CCWGG	4 cut(s)	87, 181, 1608, 1708
AjuI	GAANNNNNNNTTGG	4 cut(s)	681, 713, 2484, 2516
AloI	GAACNNNNNNTCC	2 cut(s)	2420, 2452
Alw21I	GWGCWC	3 cut(s)	120, 1846, 2332
Alw26I	GTCTC	7 cut(s)	294, 313, 1122, 1485, 2102, 3003, 3079
AlwI	GGATC	3 cut(s)	2501, 2741, 2820
Ama87I	CYCGRG	2 cut(s)	1469, 3217
AoxI	GGCC	8 cut(s)	760, 939, 1238, 1472, 2617, 2760, 3090, 3138
ApeKI	GCWGC	5 cut(s)	356, 412, 1420, 1597, 2642
ApoI	RAATTY	8 cut(s)	907, 1021, 1721, 2141, 2162, 2355, 2766, 2954
AseI	ATTAAT	2 cut(s)	1764, 3038
Asp700I	GAANNNNTTC	1 cut(s)	1176
Asp718I	GGTACC	1 cut(s)	1635
AspLEI	GCGC	4 cut(s)	1401, 1974, 2283, 2322
AspS9I	GGNCC	7 cut(s)	761, 889, 1231, 1472, 1570, 3091, 3138
AsuC2I	CCSGG	3 cut(s)	904, 1470, 1471
AsuHPI	GGTGA	6 cut(s)	458, 1202, 1969, 2032, 2768, 2958
AsuII	TTCGAA	1 cut(s)	14
AvaI	CYCGRG	2 cut(s)	1469, 3217
AvaII	GGWCC	3 cut(s)	889, 1231, 1570
AxyI	CCTNAGG	1 cut(s)	21
BaeI	ACNNNNGTAYC	2 cut(s)	1619, 1652
BalI	TGGCCA	1 cut(s)	941
BanI	GGYRCC	1 cut(s)	1635
BanII	GRGCYC	3 cut(s)	120, 1470, 3167
BarI	GAAGNNNNNNTAC	2 cut(s)	2270, 2302
BbsI	GAAGAC	2 cut(s)	959, 2035
Bbv12I	GWGCWC	3 cut(s)	120, 1846, 2332
BbvCI	CCTCAGC	1 cut(s)	2520
BbvI	GCAGC	5 cut(s)	368, 424, 1432, 1584, 2629
BceAI	ACGGC	5 cut(s)	67, 110, 590, 951, 2604
BciT130I	CCWGG	4 cut(s)	89, 183, 1610, 1710
BciVI	GTATCC	1 cut(s)	725
BcnI	CCSGG	3 cut(s)	904, 1470, 1471
BcoDI	GTCTC	7 cut(s)	294, 313, 1122, 1485, 2102, 3003, 3079
BfaI	CTAG	4 cut(s)	1566, 1593, 1601, 3021
BfmI	CTRYAG	3 cut(s)	410, 1342, 1776
BfoI	RGCGCY	2 cut(s)	1402, 1975
BfrI	CTTAAG	1 cut(s)	533
BfuAI	ACCTGC	3 cut(s)	744, 900, 965
BfuI	GTATCC	1 cut(s)	725
BglI	GCCNNNNNGGC	1 cut(s)	365
BglII	AGATCT	1 cut(s)	1363
BisI	GCNGC	6 cut(s)	165, 357, 413, 1421, 1598, 2643
BlpI	GCTNAGC	3 cut(s)	1464, 1975, 2118
BlsI	GCNGC	6 cut(s)	166, 358, 414, 1422, 1599, 2644
BmcAI	AGTACT	1 cut(s)	1340
Bme1390I	CCNGG	7 cut(s)	89, 183, 904, 1470, 1471, 1610, 1710
Bme18I	GGWCC	3 cut(s)	889, 1231, 1570
BmeT110I	CYCGRG	2 cut(s)	1469, 3217
BmgBI	CACGTC	1 cut(s)	199
BmgT120I	GGNCC	7 cut(s)	761, 889, 1231, 1472, 1570, 3091, 3138
BmiI	GGNNCC	8 cut(s)	55, 187, 568, 1571, 1637, 1811, 2438, 2493
BmrFI	CCNGG	7 cut(s)	89, 183, 904, 1470, 1471, 1610, 1710
BmsI	GCATC	7 cut(s)	334, 467, 1672, 1684, 1939, 2116, 2143
BpiI	GAAGAC	2 cut(s)	959, 2035
BplI	GAGNNNNNCTC	4 cut(s)	1482, 1514, 2546, 2578
BpmI	CTGGAG	3 cut(s)	1509, 1770, 2316
Bpu10I	CCTNAGC	1 cut(s)	2520
Bpu1102I	GCTNAGC	3 cut(s)	1464, 1975, 2118
Bpu14I	TTCGAA	1 cut(s)	14
BpuEI	CTTGAG	1 cut(s)	1800
BpuMI	CCSGG	3 cut(s)	904, 1470, 1471
BsaAI	YACGTR	1 cut(s)	884
BsaI	GGTCTC	2 cut(s)	1122, 1485
BsaJI	CCNNGG	8 cut(s)	126, 181, 1469, 2052, 2323, 2828, 3141, 3198
BsaWI	WCCGGW	1 cut(s)	1881
BsaXI	ACNNNNNCTCC	2 cut(s)	161, 191
Bsc4I	CCNNNNNNNGG	8 cut(s)	94, 538, 769, 1078, 1207, 2501, 2598, 3173
Bse118I	RCCGGY	1 cut(s)	202
Bse1I	ACTGG	9 cut(s)	630, 644, 763, 806, 1492, 1669, 2255, 2848, 3178
Bse21I	CCTNAGG	1 cut(s)	21
Bse3DI	GCAATG	3 cut(s)	707, 1422, 2637
BseBI	CCWGG	4 cut(s)	89, 183, 1610, 1710
BseDI	CCNNGG	8 cut(s)	126, 181, 1469, 2052, 2323, 2828, 3141, 3198
BseLI	CCNNNNNNNGG	8 cut(s)	94, 538, 769, 1078, 1207, 2501, 2598, 3173
BseMI	GCAATG	3 cut(s)	707, 1422, 2637
BseNI	ACTGG	9 cut(s)	630, 644, 763, 806, 1492, 1669, 2255, 2848, 3178
BseRI	GAGGAG	5 cut(s)	107, 125, 128, 2537, 2681
BseXI	GCAGC	5 cut(s)	368, 424, 1432, 1584, 2629
BseYI	CCCAGC	1 cut(s)	557
BsgI	GTGCAG	1 cut(s)	1873
Bsh1285I	CGRYCG	2 cut(s)	207, 2893
BshFI	GGCC	8 cut(s)	762, 941, 1240, 1474, 2619, 2762, 3092, 3140
BshNI	GGYRCC	1 cut(s)	1635
BsiEI	CGRYCG	2 cut(s)	207, 2893
BsiHKAI	GWGCWC	3 cut(s)	120, 1846, 2332
BsiHKCI	CYCGRG	2 cut(s)	1469, 3217
BsiSI	CCGG	5 cut(s)	203, 903, 1237, 1470, 1882
BslFI	GGGAC	2 cut(s)	405, 2951
BslI	CCNNNNNNNGG	8 cut(s)	94, 538, 769, 1078, 1207, 2501, 2598, 3173
BsmAI	GTCTC	7 cut(s)	294, 313, 1122, 1485, 2102, 3003, 3079
BsmFI	GGGAC	2 cut(s)	405, 2951
BsmI	GAATGC	2 cut(s)	533, 2891
BsnI	GGCC	8 cut(s)	762, 941, 1240, 1474, 2619, 2762, 3092, 3140
Bso31I	GGTCTC	2 cut(s)	1122, 1485
BsoBI	CYCGRG	2 cut(s)	1469, 3217
Bsp119I	TTCGAA	1 cut(s)	14
Bsp1286I	GDGCHC	5 cut(s)	120, 1470, 1846, 2332, 3167
Bsp1407I	TGTACA	1 cut(s)	2874
Bsp143I	GATC	9 cut(s)	78, 1363, 1675, 1837, 2506, 2733, 2784, 2812, 2890
Bsp1720I	GCTNAGC	3 cut(s)	1464, 1975, 2118
Bsp19I	CCATGG	2 cut(s)	2828, 3198
BspACI	CCGC	8 cut(s)	138, 165, 1066, 2275, 2773, 2959, 3094, 3136
BspANI	GGCC	8 cut(s)	762, 941, 1240, 1474, 2619, 2762, 3092, 3140
BspHI	TCATGA	2 cut(s)	2787, 2898
BspLI	GGNNCC	8 cut(s)	55, 187, 568, 1571, 1637, 1811, 2438, 2493
BspMAI	CTGCAG	2 cut(s)	414, 1780
BspMI	ACCTGC	3 cut(s)	744, 900, 965
BspPI	GGATC	3 cut(s)	2501, 2741, 2820
BspQI	GCTCTTC	1 cut(s)	66
BspT104I	TTCGAA	1 cut(s)	14
BspT107I	GGYRCC	1 cut(s)	1635
BspTI	CTTAAG	1 cut(s)	533
BspTNI	GGTCTC	2 cut(s)	1122, 1485
BsrBI	CCGCTC	1 cut(s)	2275
BsrDI	GCAATG	3 cut(s)	707, 1422, 2637
BsrFI	RCCGGY	1 cut(s)	202
BsrGI	TGTACA	1 cut(s)	2874
BsrI	ACTGG	9 cut(s)	630, 644, 763, 806, 1492, 1669, 2255, 2848, 3178
BssAI	RCCGGY	1 cut(s)	202
BssECI	CCNNGG	8 cut(s)	126, 181, 1469, 2052, 2323, 2828, 3141, 3198
BssMI	GATC	9 cut(s)	78, 1363, 1675, 1837, 2506, 2733, 2784, 2812, 2890
BssNAI	GTATAC	1 cut(s)	1899
BssT1I	CCWWGG	5 cut(s)	2052, 2323, 2828, 3141, 3198
Bst1107I	GTATAC	1 cut(s)	1899
Bst2UI	CCWGG	4 cut(s)	89, 183, 1610, 1710
Bst4CI	ACNGT	5 cut(s)	396, 1338, 1802, 1993, 2436
Bst6I	CTCTTC	9 cut(s)	66, 231, 311, 504, 510, 2100, 2547, 2572, 3176
BstAFI	CTTAAG	1 cut(s)	533
BstAPI	GCANNNNNTGC	2 cut(s)	353, 2317
BstAUI	TGTACA	1 cut(s)	2874
BstBAI	YACGTR	1 cut(s)	884
BstBI	TTCGAA	1 cut(s)	14
BstC8I	GCNNGC	9 cut(s)	133, 840, 977, 1830, 1941, 2366, 3094, 3111, 3138
BstDSI	CCRYGG	3 cut(s)	126, 2828, 3198
BstEII	GGTNACC	1 cut(s)	446
BstENI	CCTNNNNNAGG	2 cut(s)	536, 2596
BstH2I	RGCGCY	2 cut(s)	1402, 1975
BstHHI	GCGC	4 cut(s)	1401, 1974, 2283, 2322
BstKTI	GATC	9 cut(s)	81, 1366, 1678, 1840, 2509, 2736, 2787, 2815, 2893
BstMAI	GTCTC	7 cut(s)	294, 313, 1122, 1485, 2102, 3003, 3079
BstMBI	GATC	9 cut(s)	78, 1363, 1675, 1837, 2506, 2733, 2784, 2812, 2890
BstMCI	CGRYCG	2 cut(s)	207, 2893
BstMWI	GCNNNNNNNGC	7 cut(s)	137, 353, 365, 437, 972, 1949, 2317
BstNI	CCWGG	4 cut(s)	89, 183, 1610, 1710
BstNSI	RCATGY	3 cut(s)	307, 1321, 2368
BstPI	GGTNACC	1 cut(s)	446
BstSCI	CCNGG	7 cut(s)	87, 181, 902, 1468, 1469, 1608, 1708
BstSFI	CTRYAG	3 cut(s)	410, 1342, 1776
BstV1I	GCAGC	5 cut(s)	368, 424, 1432, 1584, 2629
BstV2I	GAAGAC	2 cut(s)	959, 2035
BstX2I	RGATCY	2 cut(s)	1363, 2506
BstXI	CCANNNNNNTGG	1 cut(s)	629
BstYI	RGATCY	2 cut(s)	1363, 2506
BstZ17I	GTATAC	1 cut(s)	1899
Bsu36I	CCTNAGG	1 cut(s)	21
BsuI	GTATCC	1 cut(s)	725
BsuRI	GGCC	8 cut(s)	762, 941, 1240, 1474, 2619, 2762, 3092, 3140
BtgI	CCRYGG	3 cut(s)	126, 2828, 3198
BtrI	CACGTC	1 cut(s)	199
BtsI	GCAGTG	2 cut(s)	1025, 1773
BtsIMutI	CAGTG	3 cut(s)	1025, 1773, 1807
BveI	ACCTGC	3 cut(s)	744, 900, 965
Cac8I	GCNNGC	9 cut(s)	133, 840, 977, 1830, 1941, 2366, 3094, 3111, 3138
CciI	TCATGA	2 cut(s)	2787, 2898
CfoI	GCGC	4 cut(s)	1401, 1974, 2283, 2322
Cfr10I	RCCGGY	1 cut(s)	202
Cfr13I	GGNCC	7 cut(s)	761, 889, 1231, 1472, 1570, 3091, 3138
Cfr9I	CCCGGG	1 cut(s)	1469
Csp6I	GTAC	5 cut(s)	1083, 1339, 1636, 2432, 2875
CspCI	CAANNNNNGTGG	4 cut(s)	1648, 1683, 2466, 2501
CviQI	GTAC	5 cut(s)	1083, 1339, 1636, 2432, 2875
DpnI	GATC	9 cut(s)	80, 1365, 1677, 1839, 2508, 2735, 2786, 2814, 2892
DpnII	GATC	9 cut(s)	78, 1363, 1675, 1837, 2506, 2733, 2784, 2812, 2890
DraI	TTTAAA	1 cut(s)	2698
DrdI	GACNNNNNNGTC	2 cut(s)	158, 203
DseDI	GACNNNNNNGTC	2 cut(s)	158, 203
EaeI	YGGCCR	3 cut(s)	939, 1238, 2617
Eam1104I	CTCTTC	9 cut(s)	66, 231, 311, 504, 510, 2100, 2547, 2572, 3176
EarI	CTCTTC	9 cut(s)	66, 231, 311, 504, 510, 2100, 2547, 2572, 3176
EciI	GGCGGA	1 cut(s)	1055
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	5 cut(s)	2052, 2323, 2828, 3141, 3198
Eco24I	GRGCYC	3 cut(s)	120, 1470, 3167
Eco31I	GGTCTC	2 cut(s)	1122, 1485
Eco32I	GATATC	1 cut(s)	1606
Eco47I	GGWCC	3 cut(s)	889, 1231, 1570
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	4 cut(s)	528, 1076, 2029, 2169
Eco81I	CCTNAGG	1 cut(s)	21
Eco88I	CYCGRG	2 cut(s)	1469, 3217
Eco91I	GGTNACC	1 cut(s)	446
EcoICRI	GAGCTC	1 cut(s)	118
EcoNI	CCTNNNNNAGG	2 cut(s)	536, 2596
EcoO109I	RGGNCCY	3 cut(s)	889, 1231, 1570
EcoO65I	GGTNACC	1 cut(s)	446
EcoRI	GAATTC	3 cut(s)	1021, 2355, 2954
EcoRII	CCWGG	4 cut(s)	87, 181, 1608, 1708
EcoRV	GATATC	1 cut(s)	1606
EcoT14I	CCWWGG	5 cut(s)	2052, 2323, 2828, 3141, 3198
EcoT22I	ATGCAT	1 cut(s)	2909
EcoT38I	GRGCYC	3 cut(s)	120, 1470, 3167
ErhI	CCWWGG	5 cut(s)	2052, 2323, 2828, 3141, 3198
FalI	AAGNNNNNCTT	6 cut(s)	516, 548, 944, 976, 3125, 3157
FaqI	GGGAC	2 cut(s)	405, 2951
FauI	CCCGC	4 cut(s)	145, 2780, 3101, 3129
FauNDI	CATATG	1 cut(s)	768
FblI	GTMKAC	3 cut(s)	788, 1898, 3148
Fnu4HI	GCNGC	6 cut(s)	165, 357, 413, 1421, 1598, 2643
FriOI	GRGCYC	3 cut(s)	120, 1470, 3167
Fsp4HI	GCNGC	6 cut(s)	165, 357, 413, 1421, 1598, 2643
FspBI	CTAG	4 cut(s)	1566, 1593, 1601, 3021
GlaI	GCGC	4 cut(s)	1400, 1973, 2282, 2321
GluI	GCNGC	6 cut(s)	165, 357, 413, 1421, 1598, 2643
GsaI	CCCAGC	1 cut(s)	561
GsuI	CTGGAG	3 cut(s)	1509, 1770, 2316
HaeII	RGCGCY	2 cut(s)	1402, 1975
HaeIII	GGCC	8 cut(s)	762, 941, 1240, 1474, 2619, 2762, 3092, 3140
HapII	CCGG	5 cut(s)	203, 903, 1237, 1470, 1882
HhaI	GCGC	4 cut(s)	1401, 1974, 2283, 2322
Hin6I	GCGC	4 cut(s)	1399, 1972, 2281, 2320
HinP1I	GCGC	4 cut(s)	1399, 1972, 2281, 2320
HindIII	AAGCTT	3 cut(s)	1937, 2219, 3107
HinfI	GANTC	9 cut(s)	143, 651, 675, 814, 1562, 1960, 2294, 2461, 2824
HpaII	CCGG	5 cut(s)	203, 903, 1237, 1470, 1882
HphI	GGTGA	6 cut(s)	458, 1202, 1969, 2032, 2768, 2958
Hpy166II	GTNNAC	5 cut(s)	789, 1085, 1899, 2914, 3149
Hpy8I	GTNNAC	5 cut(s)	789, 1085, 1899, 2914, 3149
Hpy99I	CGWCG	7 cut(s)	38, 71, 152, 155, 158, 173, 194
HpyCH4III	ACNGT	5 cut(s)	396, 1338, 1802, 1993, 2436
HpyCH4IV	ACGT	4 cut(s)	198, 280, 883, 1393
HpyF10VI	GCNNNNNNNGC	7 cut(s)	137, 353, 365, 437, 972, 1949, 2317
HpySE526I	ACGT	4 cut(s)	198, 280, 883, 1393
HspAI	GCGC	4 cut(s)	1399, 1972, 2281, 2320
KpnI	GGTACC	1 cut(s)	1639
Kzo9I	GATC	9 cut(s)	78, 1363, 1675, 1837, 2506, 2733, 2784, 2812, 2890
LguI	GCTCTTC	1 cut(s)	66
LmnI	GCTCC	6 cut(s)	59, 115, 191, 784, 1555, 2335
Lsp1109I	GCAGC	5 cut(s)	368, 424, 1432, 1584, 2629
LweI	GCATC	7 cut(s)	334, 467, 1672, 1684, 1939, 2116, 2143
MaeI	CTAG	4 cut(s)	1566, 1593, 1601, 3021
MaeII	ACGT	4 cut(s)	198, 280, 883, 1393
MaeIII	GTNAC	6 cut(s)	446, 922, 1320, 1626, 2007, 2816
MalI	GATC	9 cut(s)	80, 1365, 1677, 1839, 2508, 2735, 2786, 2814, 2892
MbiI	CCGCTC	1 cut(s)	2275
MboI	GATC	9 cut(s)	78, 1363, 1675, 1837, 2506, 2733, 2784, 2812, 2890
MflI	RGATCY	2 cut(s)	1363, 2506
MhlI	GDGCHC	5 cut(s)	120, 1470, 1846, 2332, 3167
MlsI	TGGCCA	1 cut(s)	941
MluNI	TGGCCA	1 cut(s)	941
MlyI	GAGTC	3 cut(s)	137, 645, 2303
MmeI	TCCRAC	8 cut(s)	57, 72, 90, 171, 213, 1628, 2482, 2790
Mox20I	TGGCCA	1 cut(s)	941
Mph1103I	ATGCAT	1 cut(s)	2909
MroXI	GAANNNNTTC	1 cut(s)	1176
MscI	TGGCCA	1 cut(s)	941
MslI	CAYNNNNRTG	2 cut(s)	882, 1415
Msp20I	TGGCCA	1 cut(s)	941
MspA1I	CMGCKG	1 cut(s)	140
MspCI	CTTAAG	1 cut(s)	533
MspI	CCGG	5 cut(s)	203, 903, 1237, 1470, 1882
MspR9I	CCNGG	7 cut(s)	89, 183, 904, 1470, 1471, 1610, 1710
Mva1269I	GAATGC	2 cut(s)	533, 2891
MvaI	CCWGG	4 cut(s)	89, 183, 1610, 1710
MwoI	GCNNNNNNNGC	7 cut(s)	137, 353, 365, 437, 972, 1949, 2317
NciI	CCSGG	3 cut(s)	904, 1470, 1471
NcoI	CCATGG	2 cut(s)	2828, 3198
NdeI	CATATG	1 cut(s)	768
NdeII	GATC	9 cut(s)	78, 1363, 1675, 1837, 2506, 2733, 2784, 2812, 2890
NlaIV	GGNNCC	8 cut(s)	55, 187, 568, 1571, 1637, 1811, 2438, 2493
NmuCI	GTSAC	2 cut(s)	446, 1320
NsiI	ATGCAT	1 cut(s)	2909
NspI	RCATGY	3 cut(s)	307, 1321, 2368
NspV	TTCGAA	1 cut(s)	14
PaeI	GCATGC	1 cut(s)	2368
PagI	TCATGA	2 cut(s)	2787, 2898
PciSI	GCTCTTC	1 cut(s)	66
PcsI	WCGNNNNNNNCGW	3 cut(s)	168, 204, 213
PctI	GAATGC	2 cut(s)	533, 2891
PdmI	GAANNNNTTC	1 cut(s)	1176
PfeI	GAWTC	6 cut(s)	675, 814, 1562, 1960, 2461, 2824
PflFI	GACNNNGTC	2 cut(s)	101, 158
PflMI	CCANNNNNTGG	2 cut(s)	2501, 3173
PfoI	TCCNGGA	1 cut(s)	1608
PkrI	GCNGC	6 cut(s)	166, 358, 414, 1422, 1599, 2644
Ple19I	CGATCG	1 cut(s)	2893
PleI	GAGTC	3 cut(s)	137, 645, 2302
PpsI	GAGTC	3 cut(s)	137, 645, 2302
Ppu21I	YACGTR	1 cut(s)	884
PpuMI	RGGWCCY	3 cut(s)	889, 1231, 1570
PshBI	ATTAAT	2 cut(s)	1764, 3038
PsiI	TTATAA	1 cut(s)	1932
Psp124BI	GAGCTC	1 cut(s)	120
Psp5II	RGGWCCY	3 cut(s)	889, 1231, 1570
Psp6I	CCWGG	4 cut(s)	87, 181, 1608, 1708
PspEI	GGTNACC	1 cut(s)	446
PspFI	CCCAGC	1 cut(s)	557
PspGI	CCWGG	4 cut(s)	87, 181, 1608, 1708
PspN4I	GGNNCC	8 cut(s)	55, 187, 568, 1571, 1637, 1811, 2438, 2493
PspPI	GGNCC	7 cut(s)	761, 889, 1231, 1472, 1570, 3091, 3138
PspPPI	RGGWCCY	3 cut(s)	889, 1231, 1570
PsrI	GAACNNNNNNTAC	2 cut(s)	3053, 3085
PstI	CTGCAG	2 cut(s)	414, 1780
PsuI	RGATCY	2 cut(s)	1363, 2506
PsyI	GACNNNGTC	2 cut(s)	101, 158
PvuI	CGATCG	1 cut(s)	2893
RsaI	GTAC	5 cut(s)	1084, 1340, 1637, 2433, 2876
RsaNI	GTAC	5 cut(s)	1083, 1339, 1636, 2432, 2875
RseI	CAYNNNNRTG	2 cut(s)	882, 1415
SacI	GAGCTC	1 cut(s)	120
SapI	GCTCTTC	1 cut(s)	66
SatI	GCNGC	6 cut(s)	165, 357, 413, 1421, 1598, 2643
Sau3AI	GATC	9 cut(s)	78, 1363, 1675, 1837, 2506, 2733, 2784, 2812, 2890
Sau96I	GGNCC	7 cut(s)	761, 889, 1231, 1472, 1570, 3091, 3138
ScaI	AGTACT	1 cut(s)	1340
SchI	GAGTC	3 cut(s)	137, 645, 2303
ScrFI	CCNGG	7 cut(s)	89, 183, 904, 1470, 1471, 1610, 1710
SduI	GDGCHC	5 cut(s)	120, 1470, 1846, 2332, 3167
SfaNI	GCATC	7 cut(s)	334, 467, 1672, 1684, 1939, 2116, 2143
SfcI	CTRYAG	3 cut(s)	410, 1342, 1776
SfuI	TTCGAA	1 cut(s)	14
SinI	GGWCC	3 cut(s)	889, 1231, 1570
SmaI	CCCGGG	1 cut(s)	1471
SmiMI	CAYNNNNRTG	2 cut(s)	882, 1415
SmlI	CTYRAG	2 cut(s)	533, 1815
SmoI	CTYRAG	2 cut(s)	533, 1815
SphI	GCATGC	1 cut(s)	2368
SrfI	GCCCGGGC	1 cut(s)	1471
SsiI	CCGC	8 cut(s)	138, 165, 1066, 2275, 2773, 2959, 3094, 3136
SspI	AATATT	2 cut(s)	339, 1578
SspMI	CTAG	4 cut(s)	1566, 1593, 1601, 3021
SstI	GAGCTC	1 cut(s)	120
StyD4I	CCNGG	7 cut(s)	87, 181, 902, 1468, 1469, 1608, 1708
StyI	CCWWGG	5 cut(s)	2052, 2323, 2828, 3141, 3198
TaaI	ACNGT	5 cut(s)	396, 1338, 1802, 1993, 2436
TaiI	ACGT	4 cut(s)	201, 283, 886, 1396
TaqI	TCGA	5 cut(s)	14, 105, 120, 207, 2353
TatI	WGTACW	2 cut(s)	1338, 2874
TauI	GCSGC	1 cut(s)	167
TfiI	GAWTC	6 cut(s)	675, 814, 1562, 1960, 2461, 2824
TscAI	CASTG	3 cut(s)	1032, 1780, 1807
TseFI	GTSAC	2 cut(s)	446, 1320
TseI	GCWGC	5 cut(s)	356, 412, 1420, 1597, 2642
Tsp45I	GTSAC	2 cut(s)	446, 1320
TspMI	CCCGGG	1 cut(s)	1469
TspRI	CASTG	3 cut(s)	1032, 1780, 1807
Tth111I	GACNNNGTC	2 cut(s)	101, 158
Van91I	CCANNNNNTGG	2 cut(s)	2501, 3173
Vha464I	CTTAAG	1 cut(s)	533
VpaK11BI	GGWCC	3 cut(s)	889, 1231, 1570
VspI	ATTAAT	2 cut(s)	1764, 3038
XagI	CCTNNNNNAGG	2 cut(s)	536, 2596
XapI	RAATTY	8 cut(s)	907, 1021, 1721, 2141, 2162, 2355, 2766, 2954
XceI	RCATGY	3 cut(s)	307, 1321, 2368
XcmI	CCANNNNNNNNNTGG	1 cut(s)	724
XmaI	CCCGGG	1 cut(s)	1469
XmiI	GTMKAC	3 cut(s)	788, 1898, 3148
XmnI	GAANNNNTTC	1 cut(s)	1176
XspI	CTAG	4 cut(s)	1566, 1593, 1601, 3021
ZrmI	AGTACT	1 cut(s)	1340
Zsp2I	ATGCAT	1 cut(s)	2909

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.