Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0008488

Physical Location & Seq

Genomic Coordinates Reverse (-)

10722392 .. 10728866

Gene Structure 6475 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom13g14810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3141 bp

ATGGCGAGAGCTTCGAAGCCGCAGGTTTCGTCCGACGAAGCCCTATCCAACGGCTCCAATTCGTCCGACGAAGAGCCGATCAACGACCAGGAGGAGGACGAGGTCGATGAGGAGGAGCTCGAAGCCGTGGCTCGCCCCGCTGACTCCGACGACGACGAGGTCGCCGCCGACGAGAACTCCCCTGGCTCCGACGATGACGTGCCGGTCGATGAGAACGACGATGATGGTTTGGATGAAGAAGAGGATGATGAAGCTAATTTGAGTAATGCTGAAATTGGAAAACGTGAGAAGGCGAGGCTCAGAGACATGCAAAATATGAAGAGACAAAAGAACAATAAGGGGAAGGGACGGTTGAAGTATCTTCTGCAGCAAACAGAGTTATTTGCCCATTTTGCTAAAGGTGACCCATCTGCATCTCAGAAGAAAGTGAAAGGAAAAGGTCGCCATGCTTCCAAAATAACTGAAGAGGAAGAGGATGAAGAATGCCTGAAGGAGGAAGAAGATGGTTTGGCTGGGGCTGGAACCACACGGCTTTTGACACAACCTTCTTGTATTCAGGGGAAGATGAGGGATTACCAACTGGCTGGATTGAACTGGCTCATACGACTCTATGAAAATGGTATAAATGGAATCCTTGCAGATGAAATGGGGCTTGGTAAAACATTGCAAACCATCTCCTTATTGGGATACCTGCATGAGTTTAGAGGAATAACTGGTCCTCATATGGTAGTTGCTCCAAAGTCGACTCTTGGAAACTGGATGAATGAGATTCGGCGTTTCTGCCCCATCTTGCGTGCTGTGAAGTTTCTTGGTAATCCTGATGAAAGAAAGCATATACGTGAGGACCTGCTGGTTGCCGGGAATTTTGATGTATGTGTTACAAGTTTTGAAATGGCCATCAAAGAGAAGACCTGCTTACGCCGTTTTAGCTGGCGTTATATAATAATTGATGAAGCTCATCGTATCAAGAATGAGAATTCACTGCTTTCAAAAACAATGAGGCTGTATAATACTAACTTCCGCCTCCTAATTACGGGTACACCACTTCAGAATAATCTTCACGAACTATGGTCTCTGCTTAACTTTCTCCTGCCAGAGATATTTAGTTCTGCTGAAACTTTTGATGAATGGTTTCAAATATCTGGTGAGAACGACCAGCAAGAGGTTGTTCAACAGCTTCACAAGGTCCTCCGGCCATTTCTTCTTAGAAGGTTGAAGTCAGACGTTGAGAAAGGTTTGCCTCCGAAAAAAGAAACAATACTCAAAGTAGGTATGTCACAAATGCAGAAACAGTACTACAAGGCTTTATTACAAAAAGATCTTGAAGTTGTTAATGCTGGTGGAGAACGTAAGCGCCTTCTGAACATAGCAATGCAGCTTCGTAAGTGCTGTAATCATCCTTATCTTTTTCAAGGTGCTGAGCCCGGGCCTCCTTATACGACTGGAGACCATCTCATCACAAATGCTGGTAAAATGGTTCTTTTGGATAAGTTGCTTCCAAAGTTAAAGGAGCGTGATTCTAGGGTCCTAATATTTTCACAGATGACTAGGCTGCTAGATATCCTTGAAGATTATTTAATGTTACGGGGTTACCTGTATTGTCGGATTGATGGCAATACTGGCGGAGAAGATCGTGATGCTTCTATTGATGCCTTCAACAAGCCTGGAAGTGAGAAATTTGTATTTTTGCTATCGACCAGAGCTGGAGGACTTGGTATTAATCTTGCCACTGCAGATGTTGTCATCCTTTATGATAGTGATTGGAACCCTCAAGTTGATTTGCAAGCACAAGATCGTGCTCACAGGATTGGTCAAAAGAAAGAAGTTCAAGTGTTCCGGTTCTGCACTGAGTATACTATTGAAGAAAAAGTTATTGAGAGGGCTTATAAAAAGCTTGCTCTTGATGCTTTGGTGATTCAACAAGGGCGCTTAGCAGAGCAAAAAACTGTTAATAAAGATGAGTTACTTCAAATGGTGAGGTTTGGTGCTGAAATGGTCTTCAGTTCCAAGGATAGCACCATTACAGATGAGGACATAGATAGGATTATTGCTAAAGGAGAAGAGACAACTGCTGAGCTTGATGCAAAGATGAAAAAATTTACTGAAGATGCTATCAAATTTAAGATGGATGATAGTATGTTTGATGACAAGCTTGATTTCAAGAAAATTGTTAGCGATAACTGGATTGAACCACCAAAAAGAGAGCGGAAGCGCAATTACTCAGAGTCTGAATACTTTAAGCAAACAATGCGCCAAGGTGCTCCAGCAAAACCGAAAGAGCCTCGAATTCCACGCATGCCTCAATTGTTGAATGAGCTGTATGAAAAGGAAGTACGGTTCCTCATGCAAACTCATCAGAAGAATCAGTTGAAAGATACAATAGAGGTGGAGGAACCAGAAGAAGTTGGAGATCCCTTGACTGCTGAGGAGGTGGAAGAAAAGGAAAGCCTGTTAGAAGAGGGTTTCTCTACTTGGAGTAGAAGAGATTTTAATACCTTCATTAGGGGCTGTGAGAAGTATGGCCGTAATGATATAAAAAGCATTGCTGCCGAGATGGAAGGGAAAACAGAGGAGGAAGTTGAAAGATACGCAAAGGTTTTTAAAGAAAGATACAAGGAGTTAAATGATTATGATAGGATCATTAAGAACATTGAAAGAGGTGAGGCTAGAATTTCCCGCAAAGATGAGATCATGAAAGCCATTGGGAAGAAGTTGGATCGTTACAAGAATCCATGGCTGGAATTGAAAATCCAGTATGGTCAAAACAAGGGGAAGTTGTACAATGAAGAATGCGATCGTTTCATGATATGCATGGTTCACAAACTTGGGTATGGGAATTGGGACGAATTGAAGGCAGAATTCCGCACATCACCCTTGTTTCGTTTTGATTGGTTTGTGAAGTCTCGCACAACTCAGGAACTCGCTAGGAGGTGTGATACATTAATTAGATTGGTGGAGAAAGAGAACCAAGAATACGATGAGAGGGAGAGACAGGCCCGCAAAGAGAAGAAGCTTGCTAAGAGCATGACACCATCAAAGCGGGCCTTGGGTAGACAGCCAACTGAGAGCCCAAATTCTGGAAAGAAGAGGAAGCAATTAACCATGGATGATTATGTCAGCTCGGGAAAAAGGAGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006323 GO:0006325 GO:0006333 GO:0006996 GO:0007275 GO:0008150 GO:0009553 GO:0009561 GO:0009987 GO:0016043 GO:0016584 GO:0022607 GO:0031497 GO:0032501 GO:0032502 GO:0034728 GO:0040034 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044464 GO:0048229 GO:0048506 GO:0048509 GO:0048510 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051276 GO:0065007 GO:0071103 GO:0071824 GO:0071840 GO:0080134 GO:0080135 GO:1900034 GO:1900036

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DBINO HAND Helicase_C SLIDE SNF2_N

Protein Analysis

1047

Amino Acids

120.96

Weight (kDa)

5.66

Isoelectric Point (pI)

43.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	188 - 348	3.7e-09	Type III restriction enzyme, res subunit
SNF2-rel_dom	PF00176	192 - 469	1.7e-99	SNF2-related domain
Helicase_C	PF00271	491 - 604	9e-25	Helicase conserved C-terminal domain
SLIDE	PF09111	878 - 988	1.6e-43	SLIDE

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06400 AT3G06400 AT3G06400 AT5G18620 AT5G18620
fragaria_vesca	FvH4_4g17400 FvH4_4g17400
malus_domestica	MD13G1172500.v1.1 MD16G1173900.v1.1
prunus_persica	Prupe.1G140700_v2.0.a1 Prupe.1G140700_v2.0.a1 Prupe.1G140700_v2.0.a1
pyrus_communis	pycom13g14810
rosa_chinensis	RchiOBHm_Chr4g0421321
rosa_laevigata	RLG00000007697
rosa_multiflora	Rmu_sc0015713.1_g000006
rosa_roxburghii	Rroxscaffold_5G00363560
rosa_rugosa	Rorug04G0168900
rosa_samantha	Rh4AG230900 Rh4BG233900 Rh4CG245300 Rh4DG229500
rosa_wichuraiana	Rw4G020300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1887
AasI	GACNNNNNNGTC	2 cut(s)	158, 203
Acc36I	ACCTGC	4 cut(s)	13, 699, 855, 920
AccB7I	CCANNNNNTGG	1 cut(s)	3077
AccBSI	CCGCTC	1 cut(s)	2206
AccI	GTMKAC	3 cut(s)	743, 1853, 3052
AclWI	GGATC	3 cut(s)	2405, 2645, 2724
AcoI	YGGCCR	3 cut(s)	894, 1193, 2521
AcsI	RAATTY	9 cut(s)	862, 976, 1676, 2096, 2117, 2286, 2670, 2858, 3073
AcuI	CTGAAG	5 cut(s)	483, 509, 1031, 1984, 2124
AfaI	GTAC	4 cut(s)	1039, 1295, 2334, 2780
AfiI	CCNNNNNNNGG	6 cut(s)	94, 493, 1033, 1162, 2502, 3077
AjiI	CACGTC	1 cut(s)	199
AjnI	CCWGG	3 cut(s)	87, 181, 1663
AjuI	GAANNNNNNNTTGG	2 cut(s)	636, 668
AloI	GAACNNNNNNTCC	2 cut(s)	2321, 2353
Alw21I	GWGCWC	3 cut(s)	120, 1801, 2263
Alw26I	GTCTC	7 cut(s)	297, 316, 1077, 1440, 2057, 2907, 2983
AlwI	GGATC	3 cut(s)	2405, 2645, 2724
AlwNI	CAGNNNCTG	1 cut(s)	2228
Ama87I	CYCGRG	2 cut(s)	1424, 3121
AoxI	GGCC	6 cut(s)	894, 1193, 1427, 2521, 2994, 3042
ApeKI	GCWGC	4 cut(s)	367, 1375, 1552, 2546
ApoI	RAATTY	9 cut(s)	862, 976, 1676, 2096, 2117, 2286, 2670, 2858, 3073
AseI	ATTAAT	2 cut(s)	1719, 2942
Asp700I	GAANNNNTTC	1 cut(s)	1131
AspLEI	GCGC	4 cut(s)	1356, 1929, 2214, 2253
AspS9I	GGNCC	7 cut(s)	716, 844, 1186, 1427, 1525, 2995, 3042
AsuC2I	CCSGG	3 cut(s)	859, 1425, 1426
AsuHPI	GGTGA	6 cut(s)	413, 1157, 1924, 1987, 2672, 2862
AsuII	TTCGAA	1 cut(s)	14
AvaI	CYCGRG	2 cut(s)	1424, 3121
AvaII	GGWCC	4 cut(s)	716, 844, 1186, 1525
BalI	TGGCCA	1 cut(s)	896
BanII	GRGCYC	3 cut(s)	120, 1425, 3071
BarI	GAAGNNNNNNTAC	2 cut(s)	2201, 2233
BbsI	GAAGAC	2 cut(s)	914, 1990
Bbv12I	GWGCWC	3 cut(s)	120, 1801, 2263
BbvCI	CCTCAGC	1 cut(s)	2424
BbvI	GCAGC	4 cut(s)	379, 1387, 1539, 2533
BceAI	ACGGC	5 cut(s)	67, 110, 545, 906, 2508
BciT130I	CCWGG	3 cut(s)	89, 183, 1665
BciVI	GTATCC	1 cut(s)	680
BcnI	CCSGG	3 cut(s)	859, 1425, 1426
BcoDI	GTCTC	7 cut(s)	297, 316, 1077, 1440, 2057, 2907, 2983
BfaI	CTAG	5 cut(s)	1521, 1548, 1556, 2667, 2925
BfmI	CTRYAG	2 cut(s)	365, 1731
BfoI	RGCGCY	2 cut(s)	1357, 1930
BfuAI	ACCTGC	4 cut(s)	13, 699, 855, 920
BfuI	GTATCC	1 cut(s)	680
BglII	AGATCT	1 cut(s)	1318
BisI	GCNGC	6 cut(s)	20, 165, 368, 1376, 1553, 2547
BlpI	GCTNAGC	3 cut(s)	1419, 1930, 2073
BlsI	GCNGC	6 cut(s)	21, 166, 369, 1377, 1554, 2548
BmcAI	AGTACT	1 cut(s)	1295
Bme1390I	CCNGG	6 cut(s)	89, 183, 859, 1425, 1426, 1665
Bme18I	GGWCC	4 cut(s)	716, 844, 1186, 1525
BmeT110I	CYCGRG	2 cut(s)	1424, 3121
BmgBI	CACGTC	1 cut(s)	199
BmgT120I	GGNCC	7 cut(s)	716, 844, 1186, 1427, 1525, 2995, 3042
BmiI	GGNNCC	7 cut(s)	55, 187, 523, 1526, 1766, 2339, 2394
BmrFI	CCNGG	6 cut(s)	89, 183, 859, 1425, 1426, 1665
BmsI	GCATC	6 cut(s)	422, 1627, 1639, 1894, 2071, 2098
BpiI	GAAGAC	2 cut(s)	914, 1990
BplI	GAGNNNNNCTC	4 cut(s)	1437, 1469, 2450, 2482
BpmI	CTGGAG	3 cut(s)	1464, 1725, 2247
Bpu10I	CCTNAGC	1 cut(s)	2424
Bpu1102I	GCTNAGC	3 cut(s)	1419, 1930, 2073
Bpu14I	TTCGAA	1 cut(s)	14
BpuEI	CTTGAG	1 cut(s)	1755
BpuMI	CCSGG	3 cut(s)	859, 1425, 1426
BsaAI	YACGTR	1 cut(s)	839
BsaI	GGTCTC	2 cut(s)	1077, 1440
BsaJI	CCNNGG	8 cut(s)	126, 181, 1424, 2007, 2254, 2732, 3045, 3102
BsaWI	WCCGGW	1 cut(s)	1836
BsaXI	ACNNNNNCTCC	2 cut(s)	161, 191
Bsc4I	CCNNNNNNNGG	6 cut(s)	94, 493, 1033, 1162, 2502, 3077
Bse118I	RCCGGY	1 cut(s)	202
Bse1I	ACTGG	8 cut(s)	585, 599, 718, 761, 1447, 1624, 2186, 2752
Bse3DI	GCAATG	3 cut(s)	662, 1377, 2541
BseBI	CCWGG	3 cut(s)	89, 183, 1665
BseDI	CCNNGG	8 cut(s)	126, 181, 1424, 2007, 2254, 2732, 3045, 3102
BseGI	GGATG	8 cut(s)	238, 250, 481, 765, 1396, 1743, 2134, 3112
BseLI	CCNNNNNNNGG	6 cut(s)	94, 493, 1033, 1162, 2502, 3077
BseMI	GCAATG	3 cut(s)	662, 1377, 2541
BseMII	CTCAG	9 cut(s)	313, 431, 1410, 1839, 2064, 2235, 2415, 2927, 3054
BseNI	ACTGG	8 cut(s)	585, 599, 718, 761, 1447, 1624, 2186, 2752
BseRI	GAGGAG	5 cut(s)	107, 125, 128, 2441, 2585
BseXI	GCAGC	4 cut(s)	379, 1387, 1539, 2533
BseYI	CCCAGC	1 cut(s)	512
BsgI	GTGCAG	1 cut(s)	1828
Bsh1285I	CGRYCG	2 cut(s)	207, 2797
BshFI	GGCC	6 cut(s)	896, 1195, 1429, 2523, 2996, 3044
BsiEI	CGRYCG	2 cut(s)	207, 2797
BsiHKAI	GWGCWC	3 cut(s)	120, 1801, 2263
BsiHKCI	CYCGRG	2 cut(s)	1424, 3121
BsiSI	CCGG	5 cut(s)	203, 858, 1192, 1425, 1837
BslFI	GGGAC	2 cut(s)	360, 2855
BslI	CCNNNNNNNGG	6 cut(s)	94, 493, 1033, 1162, 2502, 3077
BsmAI	GTCTC	7 cut(s)	297, 316, 1077, 1440, 2057, 2907, 2983
BsmFI	GGGAC	2 cut(s)	360, 2855
BsmI	GAATGC	2 cut(s)	488, 2795
BsnI	GGCC	6 cut(s)	896, 1195, 1429, 2523, 2996, 3044
Bso31I	GGTCTC	2 cut(s)	1077, 1440
BsoBI	CYCGRG	2 cut(s)	1424, 3121
Bsp119I	TTCGAA	1 cut(s)	14
Bsp1286I	GDGCHC	5 cut(s)	120, 1425, 1801, 2263, 3071
Bsp1407I	TGTACA	1 cut(s)	2778
Bsp143I	GATC	9 cut(s)	78, 1318, 1630, 1792, 2410, 2637, 2688, 2716, 2794
Bsp1720I	GCTNAGC	3 cut(s)	1419, 1930, 2073
Bsp19I	CCATGG	2 cut(s)	2732, 3102
BspANI	GGCC	6 cut(s)	896, 1195, 1429, 2523, 2996, 3044
BspCNI	CTCAG	9 cut(s)	312, 430, 1411, 1840, 2065, 2234, 2416, 2926, 3055
BspHI	TCATGA	2 cut(s)	2691, 2802
BspLI	GGNNCC	7 cut(s)	55, 187, 523, 1526, 1766, 2339, 2394
BspMAI	CTGCAG	2 cut(s)	369, 1735
BspMI	ACCTGC	4 cut(s)	13, 699, 855, 920
BspPI	GGATC	3 cut(s)	2405, 2645, 2724
BspQI	GCTCTTC	1 cut(s)	66
BspT104I	TTCGAA	1 cut(s)	14
BspTNI	GGTCTC	2 cut(s)	1077, 1440
BsrBI	CCGCTC	1 cut(s)	2206
BsrDI	GCAATG	3 cut(s)	662, 1377, 2541
BsrFI	RCCGGY	1 cut(s)	202
BsrGI	TGTACA	1 cut(s)	2778
BsrI	ACTGG	8 cut(s)	585, 599, 718, 761, 1447, 1624, 2186, 2752
BssAI	RCCGGY	1 cut(s)	202
BssECI	CCNNGG	8 cut(s)	126, 181, 1424, 2007, 2254, 2732, 3045, 3102
BssMI	GATC	9 cut(s)	78, 1318, 1630, 1792, 2410, 2637, 2688, 2716, 2794
BssNAI	GTATAC	1 cut(s)	1854
BssT1I	CCWWGG	5 cut(s)	2007, 2254, 2732, 3045, 3102
Bst1107I	GTATAC	1 cut(s)	1854
Bst2UI	CCWGG	3 cut(s)	89, 183, 1665
Bst4CI	ACNGT	4 cut(s)	351, 1293, 1948, 2337
Bst6I	CTCTTC	9 cut(s)	66, 234, 314, 459, 465, 2055, 2451, 2476, 3080
BstAPI	GCANNNNNTGC	1 cut(s)	2248
BstAUI	TGTACA	1 cut(s)	2778
BstBAI	YACGTR	1 cut(s)	839
BstBI	TTCGAA	1 cut(s)	14
BstC8I	GCNNGC	9 cut(s)	133, 795, 932, 1785, 1896, 2297, 2998, 3015, 3042
BstDSI	CCRYGG	3 cut(s)	126, 2732, 3102
BstEII	GGTNACC	2 cut(s)	401, 1589
BstENI	CCTNNNNNAGG	2 cut(s)	491, 2500
BstF5I	GGATG	8 cut(s)	238, 250, 481, 765, 1396, 1743, 2134, 3112
BstH2I	RGCGCY	2 cut(s)	1357, 1930
BstHHI	GCGC	4 cut(s)	1356, 1929, 2214, 2253
BstKTI	GATC	9 cut(s)	81, 1321, 1633, 1795, 2413, 2640, 2691, 2719, 2797
BstMAI	GTCTC	7 cut(s)	297, 316, 1077, 1440, 2057, 2907, 2983
BstMBI	GATC	9 cut(s)	78, 1318, 1630, 1792, 2410, 2637, 2688, 2716, 2794
BstMCI	CGRYCG	2 cut(s)	207, 2797
BstMWI	GCNNNNNNNGC	6 cut(s)	137, 392, 927, 1620, 1904, 2248
BstNI	CCWGG	3 cut(s)	89, 183, 1665
BstNSI	RCATGY	2 cut(s)	310, 2299
BstPI	GGTNACC	2 cut(s)	401, 1589
BstSCI	CCNGG	6 cut(s)	87, 181, 857, 1423, 1424, 1663
BstSFI	CTRYAG	2 cut(s)	365, 1731
BstV1I	GCAGC	4 cut(s)	379, 1387, 1539, 2533
BstV2I	GAAGAC	2 cut(s)	914, 1990
BstX2I	RGATCY	2 cut(s)	1318, 2410
BstXI	CCANNNNNNTGG	1 cut(s)	584
BstYI	RGATCY	2 cut(s)	1318, 2410
BstZ17I	GTATAC	1 cut(s)	1854
BsuI	GTATCC	1 cut(s)	680
BsuRI	GGCC	6 cut(s)	896, 1195, 1429, 2523, 2996, 3044
BtgI	CCRYGG	3 cut(s)	126, 2732, 3102
BtrI	CACGTC	1 cut(s)	199
BtsCI	GGATG	8 cut(s)	238, 250, 481, 765, 1396, 1743, 2134, 3112
BtsI	GCAGTG	2 cut(s)	980, 1728
BtsIMutI	CAGTG	3 cut(s)	980, 1728, 1845
BveI	ACCTGC	4 cut(s)	13, 699, 855, 920
Cac8I	GCNNGC	9 cut(s)	133, 795, 932, 1785, 1896, 2297, 2998, 3015, 3042
CaiI	CAGNNNCTG	1 cut(s)	2228
CciI	TCATGA	2 cut(s)	2691, 2802
CfoI	GCGC	4 cut(s)	1356, 1929, 2214, 2253
Cfr10I	RCCGGY	1 cut(s)	202
Cfr13I	GGNCC	7 cut(s)	716, 844, 1186, 1427, 1525, 2995, 3042
Cfr9I	CCCGGG	1 cut(s)	1424
Csp6I	GTAC	4 cut(s)	1038, 1294, 2333, 2779
CspCI	CAANNNNNGTGG	2 cut(s)	2367, 2402
CviQI	GTAC	4 cut(s)	1038, 1294, 2333, 2779
DpnI	GATC	9 cut(s)	80, 1320, 1632, 1794, 2412, 2639, 2690, 2718, 2796
DpnII	GATC	9 cut(s)	78, 1318, 1630, 1792, 2410, 2637, 2688, 2716, 2794
DraI	TTTAAA	1 cut(s)	2602
DrdI	GACNNNNNNGTC	2 cut(s)	158, 203
DseDI	GACNNNNNNGTC	2 cut(s)	158, 203
EaeI	YGGCCR	3 cut(s)	894, 1193, 2521
Eam1104I	CTCTTC	9 cut(s)	66, 234, 314, 459, 465, 2055, 2451, 2476, 3080
EarI	CTCTTC	9 cut(s)	66, 234, 314, 459, 465, 2055, 2451, 2476, 3080
EciI	GGCGGA	2 cut(s)	1010, 1638
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	5 cut(s)	2007, 2254, 2732, 3045, 3102
Eco24I	GRGCYC	3 cut(s)	120, 1425, 3071
Eco31I	GGTCTC	2 cut(s)	1077, 1440
Eco32I	GATATC	1 cut(s)	1561
Eco47I	GGWCC	4 cut(s)	716, 844, 1186, 1525
Eco53kI	GAGCTC	1 cut(s)	118
Eco57I	CTGAAG	5 cut(s)	483, 509, 1031, 1984, 2124
Eco88I	CYCGRG	2 cut(s)	1424, 3121
Eco91I	GGTNACC	2 cut(s)	401, 1589
EcoICRI	GAGCTC	1 cut(s)	118
EcoNI	CCTNNNNNAGG	2 cut(s)	491, 2500
EcoO109I	RGGNCCY	3 cut(s)	844, 1186, 1525
EcoO65I	GGTNACC	2 cut(s)	401, 1589
EcoRI	GAATTC	3 cut(s)	976, 2286, 2858
EcoRII	CCWGG	3 cut(s)	87, 181, 1663
EcoRV	GATATC	1 cut(s)	1561
EcoT14I	CCWWGG	5 cut(s)	2007, 2254, 2732, 3045, 3102
EcoT22I	ATGCAT	1 cut(s)	2813
EcoT38I	GRGCYC	3 cut(s)	120, 1425, 3071
ErhI	CCWWGG	5 cut(s)	2007, 2254, 2732, 3045, 3102
FalI	AAGNNNNNCTT	4 cut(s)	899, 931, 3029, 3061
FaqI	GGGAC	2 cut(s)	360, 2855
FauI	CCCGC	4 cut(s)	145, 2684, 3005, 3033
FauNDI	CATATG	1 cut(s)	723
FblI	GTMKAC	3 cut(s)	743, 1853, 3052
Fnu4HI	GCNGC	6 cut(s)	20, 165, 368, 1376, 1553, 2547
FokI	GGATG	8 cut(s)	245, 257, 488, 772, 1383, 1730, 2141, 3119
FriOI	GRGCYC	3 cut(s)	120, 1425, 3071
Fsp4HI	GCNGC	6 cut(s)	20, 165, 368, 1376, 1553, 2547
FspBI	CTAG	5 cut(s)	1521, 1548, 1556, 2667, 2925
GlaI	GCGC	4 cut(s)	1355, 1928, 2213, 2252
GluI	GCNGC	6 cut(s)	20, 165, 368, 1376, 1553, 2547
GsaI	CCCAGC	1 cut(s)	516
GsuI	CTGGAG	3 cut(s)	1464, 1725, 2247
HaeII	RGCGCY	2 cut(s)	1357, 1930
HaeIII	GGCC	6 cut(s)	896, 1195, 1429, 2523, 2996, 3044
HapII	CCGG	5 cut(s)	203, 858, 1192, 1425, 1837
HhaI	GCGC	4 cut(s)	1356, 1929, 2214, 2253
Hin6I	GCGC	4 cut(s)	1354, 1927, 2212, 2251
HinP1I	GCGC	4 cut(s)	1354, 1927, 2212, 2251
HincII	GTYRAC	1 cut(s)	744
HindII	GTYRAC	1 cut(s)	744
HindIII	AAGCTT	3 cut(s)	1892, 2150, 3011
HpaII	CCGG	5 cut(s)	203, 858, 1192, 1425, 1837
HphI	GGTGA	6 cut(s)	413, 1157, 1924, 1987, 2672, 2862
Hpy166II	GTNNAC	5 cut(s)	744, 1040, 1854, 2818, 3053
Hpy8I	GTNNAC	5 cut(s)	744, 1040, 1854, 2818, 3053
Hpy99I	CGWCG	8 cut(s)	38, 71, 152, 155, 158, 173, 194, 221
HpyCH4III	ACNGT	4 cut(s)	351, 1293, 1948, 2337
HpyCH4IV	ACGT	5 cut(s)	198, 283, 838, 1224, 1348
HpyF10VI	GCNNNNNNNGC	6 cut(s)	137, 392, 927, 1620, 1904, 2248
HpySE526I	ACGT	5 cut(s)	198, 283, 838, 1224, 1348
HspAI	GCGC	4 cut(s)	1354, 1927, 2212, 2251
Kzo9I	GATC	9 cut(s)	78, 1318, 1630, 1792, 2410, 2637, 2688, 2716, 2794
LguI	GCTCTTC	1 cut(s)	66
LmnI	GCTCC	6 cut(s)	59, 115, 191, 739, 1510, 2266
Lsp1109I	GCAGC	4 cut(s)	379, 1387, 1539, 2533
LweI	GCATC	6 cut(s)	422, 1627, 1639, 1894, 2071, 2098
MaeI	CTAG	5 cut(s)	1521, 1548, 1556, 2667, 2925
MaeII	ACGT	5 cut(s)	198, 283, 838, 1224, 1348
MaeIII	GTNAC	7 cut(s)	401, 877, 1275, 1581, 1589, 1962, 2720
MalI	GATC	9 cut(s)	80, 1320, 1632, 1794, 2412, 2639, 2690, 2718, 2796
MbiI	CCGCTC	1 cut(s)	2206
MboI	GATC	9 cut(s)	78, 1318, 1630, 1792, 2410, 2637, 2688, 2716, 2794
MfeI	CAATTG	1 cut(s)	2303
MflI	RGATCY	2 cut(s)	1318, 2410
MhlI	GDGCHC	5 cut(s)	120, 1425, 1801, 2263, 3071
MlsI	TGGCCA	1 cut(s)	896
MluNI	TGGCCA	1 cut(s)	896
MlyI	GAGTC	4 cut(s)	137, 600, 739, 2234
MmeI	TCCRAC	8 cut(s)	57, 72, 90, 171, 213, 1583, 2386, 2694
Mox20I	TGGCCA	1 cut(s)	896
Mph1103I	ATGCAT	1 cut(s)	2813
MroXI	GAANNNNTTC	1 cut(s)	1131
MscI	TGGCCA	1 cut(s)	896
MslI	CAYNNNNRTG	2 cut(s)	837, 1370
Msp20I	TGGCCA	1 cut(s)	896
MspA1I	CMGCKG	1 cut(s)	140
MspI	CCGG	5 cut(s)	203, 858, 1192, 1425, 1837
MspR9I	CCNGG	6 cut(s)	89, 183, 859, 1425, 1426, 1665
MunI	CAATTG	1 cut(s)	2303
Mva1269I	GAATGC	2 cut(s)	488, 2795
MvaI	CCWGG	3 cut(s)	89, 183, 1665
MwoI	GCNNNNNNNGC	6 cut(s)	137, 392, 927, 1620, 1904, 2248
NciI	CCSGG	3 cut(s)	859, 1425, 1426
NcoI	CCATGG	2 cut(s)	2732, 3102
NdeI	CATATG	1 cut(s)	723
NdeII	GATC	9 cut(s)	78, 1318, 1630, 1792, 2410, 2637, 2688, 2716, 2794
NlaIV	GGNNCC	7 cut(s)	55, 187, 523, 1526, 1766, 2339, 2394
NmeAIII	GCCGAG	1 cut(s)	2575
NmuCI	GTSAC	2 cut(s)	401, 1275
NsiI	ATGCAT	1 cut(s)	2813
NspI	RCATGY	2 cut(s)	310, 2299
NspV	TTCGAA	1 cut(s)	14
PaeI	GCATGC	1 cut(s)	2299
PagI	TCATGA	2 cut(s)	2691, 2802
PciSI	GCTCTTC	1 cut(s)	66
PcsI	WCGNNNNNNNCGW	3 cut(s)	168, 204, 213
PctI	GAATGC	2 cut(s)	488, 2795
PdmI	GAANNNNTTC	1 cut(s)	1131
PfeI	GAWTC	6 cut(s)	630, 769, 1517, 1915, 2362, 2728
PflFI	GACNNNGTC	2 cut(s)	101, 158
PflMI	CCANNNNNTGG	1 cut(s)	3077
PkrI	GCNGC	6 cut(s)	21, 166, 369, 1377, 1554, 2548
Ple19I	CGATCG	1 cut(s)	2797
PleI	GAGTC	4 cut(s)	137, 600, 739, 2233
PpsI	GAGTC	4 cut(s)	137, 600, 739, 2233
Ppu21I	YACGTR	1 cut(s)	839
PpuMI	RGGWCCY	3 cut(s)	844, 1186, 1525
PshBI	ATTAAT	2 cut(s)	1719, 2942
PsiI	TTATAA	1 cut(s)	1887
Psp124BI	GAGCTC	1 cut(s)	120
Psp5II	RGGWCCY	3 cut(s)	844, 1186, 1525
Psp6I	CCWGG	3 cut(s)	87, 181, 1663
PspEI	GGTNACC	2 cut(s)	401, 1589
PspFI	CCCAGC	1 cut(s)	512
PspGI	CCWGG	3 cut(s)	87, 181, 1663
PspN4I	GGNNCC	7 cut(s)	55, 187, 523, 1526, 1766, 2339, 2394
PspPI	GGNCC	7 cut(s)	716, 844, 1186, 1427, 1525, 2995, 3042
PspPPI	RGGWCCY	3 cut(s)	844, 1186, 1525
PsrI	GAACNNNNNNTAC	2 cut(s)	2957, 2989
PstI	CTGCAG	2 cut(s)	369, 1735
PstNI	CAGNNNCTG	1 cut(s)	2228
PsuI	RGATCY	2 cut(s)	1318, 2410
PsyI	GACNNNGTC	2 cut(s)	101, 158
PvuI	CGATCG	1 cut(s)	2797
RsaI	GTAC	4 cut(s)	1039, 1295, 2334, 2780
RsaNI	GTAC	4 cut(s)	1038, 1294, 2333, 2779
RseI	CAYNNNNRTG	2 cut(s)	837, 1370
SacI	GAGCTC	1 cut(s)	120
SalI	GTCGAC	1 cut(s)	742
SapI	GCTCTTC	1 cut(s)	66
SatI	GCNGC	6 cut(s)	20, 165, 368, 1376, 1553, 2547
Sau3AI	GATC	9 cut(s)	78, 1318, 1630, 1792, 2410, 2637, 2688, 2716, 2794
Sau96I	GGNCC	7 cut(s)	716, 844, 1186, 1427, 1525, 2995, 3042
ScaI	AGTACT	1 cut(s)	1295
SchI	GAGTC	4 cut(s)	137, 600, 739, 2234
ScrFI	CCNGG	6 cut(s)	89, 183, 859, 1425, 1426, 1665
SduI	GDGCHC	5 cut(s)	120, 1425, 1801, 2263, 3071
SfaNI	GCATC	6 cut(s)	422, 1627, 1639, 1894, 2071, 2098
SfcI	CTRYAG	2 cut(s)	365, 1731
SfuI	TTCGAA	1 cut(s)	14
SinI	GGWCC	4 cut(s)	716, 844, 1186, 1525
SmaI	CCCGGG	1 cut(s)	1426
SmiMI	CAYNNNNRTG	2 cut(s)	837, 1370
SmlI	CTYRAG	1 cut(s)	1770
SmoI	CTYRAG	1 cut(s)	1770
SphI	GCATGC	1 cut(s)	2299
SrfI	GCCCGGGC	1 cut(s)	1426
SspI	AATATT	1 cut(s)	1533
SspMI	CTAG	5 cut(s)	1521, 1548, 1556, 2667, 2925
SstI	GAGCTC	1 cut(s)	120
StyD4I	CCNGG	6 cut(s)	87, 181, 857, 1423, 1424, 1663
StyI	CCWWGG	5 cut(s)	2007, 2254, 2732, 3045, 3102
TaaI	ACNGT	4 cut(s)	351, 1293, 1948, 2337
TaiI	ACGT	5 cut(s)	201, 286, 841, 1227, 1351
TaqI	TCGA	7 cut(s)	14, 105, 120, 207, 743, 1694, 2284
TatI	WGTACW	2 cut(s)	1293, 2778
TauI	GCSGC	2 cut(s)	22, 167
TfiI	GAWTC	6 cut(s)	630, 769, 1517, 1915, 2362, 2728
TscAI	CASTG	3 cut(s)	987, 1735, 1852
TseFI	GTSAC	2 cut(s)	401, 1275
TseI	GCWGC	4 cut(s)	367, 1375, 1552, 2546
Tsp45I	GTSAC	2 cut(s)	401, 1275
TspMI	CCCGGG	1 cut(s)	1424
TspRI	CASTG	3 cut(s)	987, 1735, 1852
Tth111I	GACNNNGTC	2 cut(s)	101, 158
Van91I	CCANNNNNTGG	1 cut(s)	3077
VpaK11BI	GGWCC	4 cut(s)	716, 844, 1186, 1525
VspI	ATTAAT	2 cut(s)	1719, 2942
XagI	CCTNNNNNAGG	2 cut(s)	491, 2500
XapI	RAATTY	9 cut(s)	862, 976, 1676, 2096, 2117, 2286, 2670, 2858, 3073
XceI	RCATGY	2 cut(s)	310, 2299
XcmI	CCANNNNNNNNNTGG	1 cut(s)	679
XmaI	CCCGGG	1 cut(s)	1424
XmiI	GTMKAC	3 cut(s)	743, 1853, 3052
XmnI	GAANNNNTTC	1 cut(s)	1131
XspI	CTAG	5 cut(s)	1521, 1548, 1556, 2667, 2925
ZrmI	AGTACT	1 cut(s)	1295
Zsp2I	ATGCAT	1 cut(s)	2813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.