Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0008978

Physical Location & Seq

Genomic Coordinates Reverse (-)

22350043 .. 22355418

Gene Structure 5376 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g18500.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGGGTAGCAACGTTAAAGGAAGGACGGTTCTTGAGGTGGGAGCTGATGGGGTGGCTCTCATCACCATCATCAACCCTCCTGTCAATTCCCTCTCCTTTGATGTGTTGTATGGCTTGAAAGACAGCTATGAGGAGGCGATGAGGAGAGATGATGTCAAGGCAATTGTCGTTACAGGTGCGAAAGGGAAATTCTCTGGCGGATTTGATATTAGTGCTTTCGGTGGGCTACAAGGCGGGCATAAAGCGGAGCCGAAGCCTGGTTATATATCTGTGGAGGTTATCACTAACGCTGTCGAGGGTGCTAGGAAACCTTCAGTTGCTGCCATTGATGGCCTGGCCTTGGGGGGAGGACTAGAGGTTGCAATGGCTTGCCATGCCCGGATATCTACTCCGACTGCGCAATTAGGCTTGCCAGAACTTCAGCTTGGACTCATTCCTGGATTTGGAGGAACACAACGGCTTCCACGCCTTGTTGGAATCTCAAAGGCACTTGAAATGATGCTGACTTCAAAACCGGTTAAAGGGGAAGAGGCTCATGATTTAGGCCTTGTTGATGCTTTAGTTTCACCTGATGAGTTGGTAAGCACTGCACGTAGGTGGGCCTTGGATATCTTGGAGCGTCGGAGACCATGGGTTGCTAGTCTTCATAAGACTGACAAGTTAGAACCTCTCGGTGAAGCTAGGGAAATACTTAAATTTGCAAGAGCTCAAGTACAGAAGCAGGCTCCCAATCTCAAACATCCATTGGTTTGCATCGATGTTGTTGAAGAGGGTATAGCTTCTGGTGGCCGGGCTGGGCTTTGGAAGGAAGCTGAAGAATTTCAAGGACTTCTACGTGCTGACACTTGCAAGAGCTTGGTCCACATCTTCTTTGCTCAACGTGGAACCTCAAAGGTACCTGGAATTACTGATCGAGGTTTAATGCCGAGACGAGTGACTAAGGTTGCTGTCATTGGTGGAGGGTTGATGGGCTCTGGAATAACCACAGCGTTGATTCTTAGTAATTATCCAGTGATCCTAAAAGAAGTAAATGAGAAGTTCTTGCAGGCTGGGATTGGTAGAGTCAAAGCCAATCTGCAGAGCCGTGTCAAGAAAGGGAAAATGACTCCAGAAAAGTTTGAAAAGACATTTGCTCTTCTTAAAGGTGCCCTGGACTATGAAAGCTTTAGGGATGTTGACATGGTCATAGAGGCGGTTATTGAGAAGGTTTCTTTAAAGCAAGAAATTTTTGCTGACCTCGAAAAGTATTGCCCACAACACTGCATACTTGCTAGTAACACTTCCACAATCGACTTGAACCTTATCGGAGAAAAGACCAAATCCCATGATAGAATTATTGGAGCCCATTTCTTTAGTCCAGCACATATCATGCCACTTTTGGAAATTGTTCGCACTAATAGGACATCTCCACAAGTTATTGTTGATTTGCTAGAAGTTGGGAAGAAGATAAAGAAAACCCCGGTTGTTGTTGGGAACTGCACTGGATTTGCTGTTAACAGAATGTTCTTTCCTTATACCCAAGCTGGTCTTCTGCTTATTGAGCGTGGTGCAGATGTATATCAGATTGACAGGGCGATCACCAAATTTGGAATGCCCATGGGCCCATTCAGATTGGCTGATTTGGTTGGCTTTGGTGTGGCGATTGCAACTGGCATGCAATTTATTGAGAATTTTCCTGAGCGGACATATAAGTCGATGCTTATCCCCATGATGCAGGAGGATGGGAGAGCAGGTGAAACTACAAAAAGAGGGTTCTATGTTTATGATGACAAACGCCAAGCTAAACCAGATCCTGAATTGAAGAAATACATTGAGAAGTCAAGGAGCATTTCTGGGCTTTCAGTAGATGCTAAGCTAGTGAAGTTACCAGAAAAGGACATCGTGGAAATGATCTTTTTCCCTGTTGTGAACGAGGCTTGCAGAGTGTATGCTGAAGGTATTGCCGTAAAAGCTGCTGACCTTGATATTGCTTCAGTCATGGGCATGGGTTTTCCACCTTACAGGGGAGGTATCATGTTCTGGGCCGATTTAGTTGGATCCAAATACATATACTCAAGATTGGAGCAATGGTCAAATACCTACGGGGAATTTTTCAAGCCTTGTGCCTATTTGGCTGAAAGAGCTGCACAGGGAGCTCCTCTGAGCTCTCCTTTGAAGCAATCAAAATCTCGGTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004165 GO:0004300 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009062 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

726

Amino Acids

79.19

Weight (kDa)

9.07

Isoelectric Point (pI)

35.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	16 - 217	3.7e-40	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	28 - 196	1.3e-23	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	314 - 492	1.5e-60	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	495 - 588	3e-21	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008978)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06860
fragaria_vesca	FvH4_4g18500 FvH4_4g18500 FvH4_4g18500
malus_domestica	MD13G1166700.v1.1 MD16G1166400.v1.1
prunus_persica	Prupe.1G133000_v2.0.a1
pyrus_communis	pycom13g14220 pycom16g14060
rosa_chinensis	RchiOBHm_Chr4g0423051
rosa_laevigata	RLG00000007590
rosa_multiflora	Rmu_co8121856.1_g000001 Rmu_sc0006639.1_g000001 Rmu_sc0023965.1_g000001 Rmu_sc0028464.1_g000006
rosa_roxburghii	Rroxscaffold_5G00365130
rosa_rugosa	Rorug04G0183300.1
rosa_samantha	Rh4AG243100 Rh4BG247800 Rh4CG257600 Rh4DG242800
rosa_wichuraiana	Rw4G020970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2176
AarI	CACCTGC	1 cut(s)	1721
AasI	GACNNNNNNGTC	1 cut(s)	1690
Acc16I	TGCGCA	1 cut(s)	399
Acc36I	ACCTGC	1 cut(s)	1721
Acc65I	GGTACC	1 cut(s)	895
AccB1I	GGYRCC	2 cut(s)	895, 1145
AccBSI	CCGCTC	1 cut(s)	1681
AciI	CCGC	5 cut(s)	198, 234, 245, 1193, 1681
AclI	AACGTT	1 cut(s)	12
AclWI	GGATC	4 cut(s)	1009, 1784, 2031, 2044
AcoI	YGGCCR	1 cut(s)	787
AcsI	RAATTY	7 cut(s)	188, 695, 818, 1224, 1583, 1669, 2087
AcuI	CTGAAG	5 cut(s)	297, 404, 834, 1953, 1956
AfaI	GTAC	2 cut(s)	714, 897
AfiI	CCNNNNNNNGG	5 cut(s)	257, 340, 443, 521, 2003
AgeI	ACCGGT	1 cut(s)	514
AjnI	CCWGG	5 cut(s)	256, 333, 436, 898, 1149
AjuI	GAANNNNNNNTTGG	4 cut(s)	408, 440, 1512, 1544
AleI	CACNNNNGTG	1 cut(s)	595
AloI	GAACNNNNNNTCC	2 cut(s)	12, 44
Alw21I	GWGCWC	3 cut(s)	709, 2137, 2147
Alw26I	GTCTC	2 cut(s)	619, 922
AlwI	GGATC	4 cut(s)	1009, 1784, 2031, 2044
AlwNI	CAGNNNCTG	2 cut(s)	320, 1793
AoxI	GGCC	7 cut(s)	331, 336, 544, 600, 787, 1600, 2022
ApaI	GGGCCC	1 cut(s)	1604
ApeKI	GCWGC	3 cut(s)	320, 1952, 2123
ApoI	RAATTY	7 cut(s)	188, 695, 818, 1224, 1583, 1669, 2087
AsiGI	ACCGGT	1 cut(s)	514
Asp700I	GAANNNNTTC	2 cut(s)	819, 1386
Asp718I	GGTACC	1 cut(s)	895
AspLEI	GCGC	1 cut(s)	400
AspS9I	GGNCC	5 cut(s)	600, 859, 1600, 1601, 2022
AsuC2I	CCSGG	3 cut(s)	379, 791, 1460
AsuHPI	GGTGA	5 cut(s)	55, 558, 686, 1570, 1745
AvaII	GGWCC	1 cut(s)	859
BaeGI	GKGCMC	2 cut(s)	1150, 1604
BamHI	GGATCC	1 cut(s)	2036
BanI	GGYRCC	2 cut(s)	895, 1145
BanII	GRGCYC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
BbsI	GAAGAC	2 cut(s)	635, 1520
Bbv12I	GWGCWC	3 cut(s)	709, 2137, 2147
BbvI	GCAGC	3 cut(s)	307, 1939, 2110
BccI	CCATC	5 cut(s)	41, 74, 323, 961, 1715
BceAI	ACGGC	3 cut(s)	473, 1068, 1928
BciT130I	CCWGG	5 cut(s)	258, 335, 438, 900, 1151
BcnI	CCSGG	3 cut(s)	379, 791, 1460
BcoDI	GTCTC	2 cut(s)	619, 922
BfaI	CTAG	7 cut(s)	303, 353, 639, 681, 1272, 1430, 1856
BfmI	CTRYAG	1 cut(s)	1076
BfuAI	ACCTGC	1 cut(s)	1721
BglI	GCCNNNNNGGC	1 cut(s)	2111
BisI	GCNGC	3 cut(s)	321, 1953, 2124
BlpI	GCTNAGC	1 cut(s)	1851
BlsI	GCNGC	3 cut(s)	322, 1954, 2125
Bme1390I	CCNGG	8 cut(s)	258, 335, 379, 438, 791, 900, 1151, 1460
Bme18I	GGWCC	1 cut(s)	859
BmgT120I	GGNCC	5 cut(s)	600, 859, 1600, 1601, 2022
BmiI	GGNNCC	8 cut(s)	249, 726, 886, 897, 1147, 1342, 1602, 2038
BmrFI	CCNGG	8 cut(s)	258, 335, 379, 438, 791, 900, 1151, 1460
BmsI	GCATC	6 cut(s)	489, 544, 762, 1686, 1701, 1837
BpiI	GAAGAC	2 cut(s)	635, 1520
BpmI	CTGGAG	1 cut(s)	1092
Bpu10I	CCTNAGC	1 cut(s)	1677
Bpu1102I	GCTNAGC	1 cut(s)	1851
BpuEI	CTTGAG	3 cut(s)	53, 693, 2038
BpuMI	CCSGG	3 cut(s)	379, 791, 1460
Bsa29I	ATCGAT	1 cut(s)	756
BsaAI	YACGTR	2 cut(s)	593, 836
BsaBI	GATNNNNATC	2 cut(s)	1557, 1700
BsaI	GGTCTC	1 cut(s)	619
BsaJI	CCNNGG	6 cut(s)	339, 603, 629, 1149, 1458, 1596
BsaWI	WCCGGW	1 cut(s)	514
BsaXI	ACNNNNNCTCC	4 cut(s)	1717, 1747, 2054, 2084
Bsc4I	CCNNNNNNNGG	5 cut(s)	257, 340, 443, 521, 2003
Bse118I	RCCGGY	1 cut(s)	514
Bse1I	ACTGG	3 cut(s)	1010, 1486, 1654
Bse3DI	GCAATG	2 cut(s)	369, 2072
Bse8I	GATNNNNATC	2 cut(s)	1557, 1700
BseBI	CCWGG	5 cut(s)	258, 335, 438, 900, 1151
BseCI	ATCGAT	1 cut(s)	756
BseDI	CCNNGG	6 cut(s)	339, 603, 629, 1149, 1458, 1596
BseGI	GGATG	3 cut(s)	739, 1177, 1726
BseJI	GATNNNNATC	2 cut(s)	1557, 1700
BseLI	CCNNNNNNNGG	5 cut(s)	257, 340, 443, 521, 2003
BseMI	GCAATG	2 cut(s)	369, 2072
BseMII	CTCAG	2 cut(s)	1668, 2132
BseNI	ACTGG	3 cut(s)	1010, 1486, 1654
BseRI	GAGGAG	3 cut(s)	146, 157, 2127
BseSI	GKGCMC	2 cut(s)	1150, 1604
BseXI	GCAGC	3 cut(s)	307, 1939, 2110
BseYI	CCCAGC	2 cut(s)	794, 1049
BsgI	GTGCAG	4 cut(s)	573, 1462, 1569, 2109
BshFI	GGCC	7 cut(s)	333, 338, 546, 602, 789, 1602, 2024
BshNI	GGYRCC	2 cut(s)	895, 1145
BshTI	ACCGGT	1 cut(s)	514
BshVI	ATCGAT	1 cut(s)	756
BsiHKAI	GWGCWC	3 cut(s)	709, 2137, 2147
BsiSI	CCGG	4 cut(s)	379, 515, 790, 1460
BslI	CCNNNNNNNGG	5 cut(s)	257, 340, 443, 521, 2003
BsmAI	GTCTC	2 cut(s)	619, 922
BsmBI	CGTCTC	1 cut(s)	922
BsmI	GAATGC	1 cut(s)	1596
BsnI	GGCC	7 cut(s)	333, 338, 546, 602, 789, 1602, 2024
Bso31I	GGTCTC	1 cut(s)	619
Bsp120I	GGGCCC	1 cut(s)	1600
Bsp1286I	GDGCHC	7 cut(s)	709, 974, 1150, 1345, 1604, 2137, 2147
Bsp143I	GATC	6 cut(s)	910, 1014, 1575, 1789, 1890, 2036
Bsp1720I	GCTNAGC	1 cut(s)	1851
Bsp19I	CCATGG	2 cut(s)	629, 1596
BspACI	CCGC	5 cut(s)	198, 234, 245, 1193, 1681
BspANI	GGCC	7 cut(s)	333, 338, 546, 602, 789, 1602, 2024
BspCNI	CTCAG	2 cut(s)	1669, 2133
BspDI	ATCGAT	1 cut(s)	756
BspHI	TCATGA	1 cut(s)	535
BspLI	GGNNCC	8 cut(s)	249, 726, 886, 897, 1147, 1342, 1602, 2038
BspMAI	CTGCAG	1 cut(s)	1080
BspMI	ACCTGC	1 cut(s)	1721
BspPI	GGATC	4 cut(s)	1009, 1784, 2031, 2044
BspQI	GCTCTTC	1 cut(s)	1140
BspT107I	GGYRCC	2 cut(s)	895, 1145
BspTNI	GGTCTC	1 cut(s)	619
BsrBI	CCGCTC	1 cut(s)	1681
BsrDI	GCAATG	2 cut(s)	369, 2072
BsrFI	RCCGGY	1 cut(s)	514
BsrI	ACTGG	3 cut(s)	1010, 1486, 1654
BssAI	RCCGGY	1 cut(s)	514
BssECI	CCNNGG	6 cut(s)	339, 603, 629, 1149, 1458, 1596
BssMI	GATC	6 cut(s)	910, 1014, 1575, 1789, 1890, 2036
BssT1I	CCWWGG	4 cut(s)	339, 603, 629, 1596
Bst2UI	CCWGG	5 cut(s)	258, 335, 438, 900, 1151
Bst4CI	ACNGT	1 cut(s)	28
Bst6I	CTCTTC	3 cut(s)	522, 762, 1140
BstBAI	YACGTR	2 cut(s)	593, 836
BstC8I	GCNNGC	7 cut(s)	236, 370, 410, 723, 1047, 1655, 1918
BstDEI	CTNAG	5 cut(s)	939, 998, 1677, 1851, 2141
BstDSI	CCRYGG	2 cut(s)	629, 1596
BstF5I	GGATG	3 cut(s)	739, 1177, 1726
BstHHI	GCGC	1 cut(s)	400
BstKTI	GATC	6 cut(s)	913, 1017, 1578, 1792, 1893, 2039
BstMAI	GTCTC	2 cut(s)	619, 922
BstMBI	GATC	6 cut(s)	910, 1014, 1575, 1789, 1890, 2036
BstMWI	GCNNNNNNNGC	6 cut(s)	374, 1540, 1949, 2111, 2120, 2132
BstNI	CCWGG	5 cut(s)	258, 335, 438, 900, 1151
BstNSI	RCATGY	1 cut(s)	1657
BstSCI	CCNGG	8 cut(s)	256, 333, 377, 436, 789, 898, 1149, 1458
BstSFI	CTRYAG	1 cut(s)	1076
BstSLI	GKGCMC	2 cut(s)	1150, 1604
BstV1I	GCAGC	3 cut(s)	307, 1939, 2110
BstV2I	GAAGAC	2 cut(s)	635, 1520
BstX2I	RGATCY	2 cut(s)	1789, 2036
BstYI	RGATCY	2 cut(s)	1789, 2036
Bsu15I	ATCGAT	1 cut(s)	756
BsuRI	GGCC	7 cut(s)	333, 338, 546, 602, 789, 1602, 2024
BsuTUI	ATCGAT	1 cut(s)	756
BtgI	CCRYGG	2 cut(s)	629, 1596
BtgZI	GCGATG	1 cut(s)	152
BtsCI	GGATG	3 cut(s)	739, 1177, 1726
BtsI	GCAGTG	2 cut(s)	585, 1258
BtsIMutI	CAGTG	4 cut(s)	585, 1017, 1258, 1479
BveI	ACCTGC	1 cut(s)	1721
Cac8I	GCNNGC	7 cut(s)	236, 370, 410, 723, 1047, 1655, 1918
CaiI	CAGNNNCTG	2 cut(s)	320, 1793
CciI	TCATGA	1 cut(s)	535
CfoI	GCGC	1 cut(s)	400
Cfr10I	RCCGGY	1 cut(s)	514
Cfr13I	GGNCC	5 cut(s)	600, 859, 1600, 1601, 2022
ClaI	ATCGAT	1 cut(s)	756
CseI	GACGC	1 cut(s)	608
Csp6I	GTAC	2 cut(s)	713, 896
CspAI	ACCGGT	1 cut(s)	514
CviQI	GTAC	2 cut(s)	713, 896
DdeI	CTNAG	5 cut(s)	939, 998, 1677, 1851, 2141
DpnI	GATC	6 cut(s)	912, 1016, 1577, 1791, 1892, 2038
DpnII	GATC	6 cut(s)	910, 1014, 1575, 1789, 1890, 2036
DraI	TTTAAA	1 cut(s)	1215
DrdI	GACNNNNNNGTC	1 cut(s)	1690
DseDI	GACNNNNNNGTC	1 cut(s)	1690
EaeI	YGGCCR	1 cut(s)	787
Eam1104I	CTCTTC	3 cut(s)	522, 762, 1140
EarI	CTCTTC	3 cut(s)	522, 762, 1140
EciI	GGCGGA	1 cut(s)	213
Ecl136II	GAGCTC	3 cut(s)	707, 2135, 2145
Eco130I	CCWWGG	4 cut(s)	339, 603, 629, 1596
Eco147I	AGGCCT	1 cut(s)	546
Eco24I	GRGCYC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
Eco31I	GGTCTC	1 cut(s)	619
Eco32I	GATATC	2 cut(s)	384, 610
Eco47I	GGWCC	1 cut(s)	859
Eco53kI	GAGCTC	3 cut(s)	707, 2135, 2145
Eco57I	CTGAAG	5 cut(s)	297, 404, 834, 1953, 1956
EcoICRI	GAGCTC	3 cut(s)	707, 2135, 2145
EcoRII	CCWGG	5 cut(s)	256, 333, 436, 898, 1149
EcoRV	GATATC	2 cut(s)	384, 610
EcoT14I	CCWWGG	4 cut(s)	339, 603, 629, 1596
EcoT38I	GRGCYC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
ErhI	CCWWGG	4 cut(s)	339, 603, 629, 1596
Esp3I	CGTCTC	1 cut(s)	922
FalI	AAGNNNNNCTT	2 cut(s)	1512, 1544
FauI	CCCGC	1 cut(s)	227
Fnu4HI	GCNGC	3 cut(s)	321, 1953, 2124
FokI	GGATG	3 cut(s)	726, 1184, 1733
FriOI	GRGCYC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
Fsp4HI	GCNGC	3 cut(s)	321, 1953, 2124
FspBI	CTAG	7 cut(s)	303, 353, 639, 681, 1272, 1430, 1856
FspI	TGCGCA	1 cut(s)	399
GlaI	GCGC	1 cut(s)	399
GluI	GCNGC	3 cut(s)	321, 1953, 2124
GsaI	CCCAGC	2 cut(s)	798, 1053
GsuI	CTGGAG	1 cut(s)	1092
HaeIII	GGCC	7 cut(s)	333, 338, 546, 602, 789, 1602, 2024
HapII	CCGG	4 cut(s)	379, 515, 790, 1460
HgaI	GACGC	1 cut(s)	608
HhaI	GCGC	1 cut(s)	400
Hin6I	GCGC	1 cut(s)	398
HinP1I	GCGC	1 cut(s)	398
HincII	GTYRAC	2 cut(s)	1177, 1495
HindII	GTYRAC	2 cut(s)	1177, 1495
HindIII	AAGCTT	1 cut(s)	1162
HinfI	GANTC	5 cut(s)	429, 477, 994, 1062, 1105
HpaI	GTTAAC	1 cut(s)	1495
HpaII	CCGG	4 cut(s)	379, 515, 790, 1460
HphI	GGTGA	5 cut(s)	55, 558, 686, 1570, 1745
Hpy166II	GTNNAC	4 cut(s)	862, 1177, 1495, 1909
Hpy188I	TCNGA	6 cut(s)	393, 624, 1307, 1563, 1610, 2142
Hpy188III	TCNNGA	8 cut(s)	32, 536, 975, 1090, 1109, 1676, 1793, 2055
Hpy8I	GTNNAC	4 cut(s)	862, 1177, 1495, 1909
Hpy99I	CGWCG	1 cut(s)	624
HpyAV	CCTTC	5 cut(s)	15, 321, 799, 1198, 1928
HpyCH4III	ACNGT	1 cut(s)	28
HpyCH4IV	ACGT	4 cut(s)	12, 592, 835, 880
HpyF10VI	GCNNNNNNNGC	6 cut(s)	374, 1540, 1949, 2111, 2120, 2132
HpyF3I	CTNAG	5 cut(s)	939, 998, 1677, 1851, 2141
HpySE526I	ACGT	4 cut(s)	12, 592, 835, 880
HspAI	GCGC	1 cut(s)	398
KpnI	GGTACC	1 cut(s)	899
KspAI	GTTAAC	1 cut(s)	1495
Kzo9I	GATC	6 cut(s)	910, 1014, 1575, 1789, 1890, 2036
LguI	GCTCTTC	1 cut(s)	1140
LmnI	GCTCC	9 cut(s)	41, 247, 616, 730, 1340, 1824, 2062, 2132, 2140
Lsp1109I	GCAGC	3 cut(s)	307, 1939, 2110
LweI	GCATC	6 cut(s)	489, 544, 762, 1686, 1701, 1837
MaeI	CTAG	7 cut(s)	303, 353, 639, 681, 1272, 1430, 1856
MaeII	ACGT	4 cut(s)	12, 592, 835, 880
MaeIII	GTNAC	4 cut(s)	169, 934, 1274, 1863
MalI	GATC	6 cut(s)	912, 1016, 1577, 1791, 1892, 2038
MbiI	CCGCTC	1 cut(s)	1681
MboI	GATC	6 cut(s)	910, 1014, 1575, 1789, 1890, 2036
MfeI	CAATTG	1 cut(s)	162
MflI	RGATCY	2 cut(s)	1789, 2036
MhlI	GDGCHC	7 cut(s)	709, 974, 1150, 1345, 1604, 2137, 2147
MlyI	GAGTC	3 cut(s)	423, 1071, 1099
MmeI	TCCRAC	4 cut(s)	416, 454, 602, 2014
MroXI	GAANNNNTTC	2 cut(s)	819, 1386
MseI	TTAA	7 cut(s)	15, 519, 693, 920, 1140, 1214, 1494
MslI	CAYNNNNRTG	2 cut(s)	595, 1982
MspI	CCGG	4 cut(s)	379, 515, 790, 1460
MspR9I	CCNGG	8 cut(s)	258, 335, 379, 438, 791, 900, 1151, 1460
MunI	CAATTG	1 cut(s)	162
Mva1269I	GAATGC	1 cut(s)	1596
MvaI	CCWGG	5 cut(s)	258, 335, 438, 900, 1151
MwoI	GCNNNNNNNGC	6 cut(s)	374, 1540, 1949, 2111, 2120, 2132
NciI	CCSGG	3 cut(s)	379, 791, 1460
NcoI	CCATGG	2 cut(s)	629, 1596
NdeII	GATC	6 cut(s)	910, 1014, 1575, 1789, 1890, 2036
NlaIV	GGNNCC	8 cut(s)	249, 726, 886, 897, 1147, 1342, 1602, 2038
NmeAIII	GCCGAG	1 cut(s)	951
NmuCI	GTSAC	1 cut(s)	934
NsbI	TGCGCA	1 cut(s)	399
NspI	RCATGY	1 cut(s)	1657
OliI	CACNNNNGTG	1 cut(s)	595
PaeI	GCATGC	1 cut(s)	1657
PagI	TCATGA	1 cut(s)	535
PaqCI	CACCTGC	1 cut(s)	1721
PceI	AGGCCT	1 cut(s)	546
PciSI	GCTCTTC	1 cut(s)	1140
PctI	GAATGC	1 cut(s)	1596
PdmI	GAANNNNTTC	2 cut(s)	819, 1386
PfeI	GAWTC	2 cut(s)	477, 994
PflFI	GACNNNGTC	1 cut(s)	1181
PfoI	TCCNGGA	1 cut(s)	436
PinAI	ACCGGT	1 cut(s)	514
PkrI	GCNGC	3 cut(s)	322, 1954, 2125
PleI	GAGTC	3 cut(s)	423, 1070, 1099
PpsI	GAGTC	3 cut(s)	423, 1070, 1099
Ppu21I	YACGTR	2 cut(s)	593, 836
PsiI	TTATAA	1 cut(s)	2176
Psp124BI	GAGCTC	3 cut(s)	709, 2137, 2147
Psp1406I	AACGTT	1 cut(s)	12
Psp6I	CCWGG	5 cut(s)	256, 333, 436, 898, 1149
PspFI	CCCAGC	2 cut(s)	794, 1049
PspGI	CCWGG	5 cut(s)	256, 333, 436, 898, 1149
PspN4I	GGNNCC	8 cut(s)	249, 726, 886, 897, 1147, 1342, 1602, 2038
PspOMI	GGGCCC	1 cut(s)	1600
PspPI	GGNCC	5 cut(s)	600, 859, 1600, 1601, 2022
PstI	CTGCAG	1 cut(s)	1080
PstNI	CAGNNNCTG	2 cut(s)	320, 1793
PsuI	RGATCY	2 cut(s)	1789, 2036
PsyI	GACNNNGTC	1 cut(s)	1181
RsaI	GTAC	2 cut(s)	714, 897
RsaNI	GTAC	2 cut(s)	713, 896
RseI	CAYNNNNRTG	2 cut(s)	595, 1982
SacI	GAGCTC	3 cut(s)	709, 2137, 2147
SapI	GCTCTTC	1 cut(s)	1140
SaqAI	TTAA	7 cut(s)	15, 519, 693, 920, 1140, 1214, 1494
SatI	GCNGC	3 cut(s)	321, 1953, 2124
Sau3AI	GATC	6 cut(s)	910, 1014, 1575, 1789, 1890, 2036
Sau96I	GGNCC	5 cut(s)	600, 859, 1600, 1601, 2022
SchI	GAGTC	3 cut(s)	423, 1071, 1099
ScrFI	CCNGG	8 cut(s)	258, 335, 379, 438, 791, 900, 1151, 1460
SduI	GDGCHC	7 cut(s)	709, 974, 1150, 1345, 1604, 2137, 2147
SfaNI	GCATC	6 cut(s)	489, 544, 762, 1686, 1701, 1837
SfcI	CTRYAG	1 cut(s)	1076
SinI	GGWCC	1 cut(s)	859
SmiMI	CAYNNNNRTG	2 cut(s)	595, 1982
SmlI	CTYRAG	3 cut(s)	32, 708, 2053
SmoI	CTYRAG	3 cut(s)	32, 708, 2053
SphI	GCATGC	1 cut(s)	1657
SseBI	AGGCCT	1 cut(s)	546
SsiI	CCGC	5 cut(s)	198, 234, 245, 1193, 1681
SspMI	CTAG	7 cut(s)	303, 353, 639, 681, 1272, 1430, 1856
SstI	GAGCTC	3 cut(s)	709, 2137, 2147
StuI	AGGCCT	1 cut(s)	546
StyD4I	CCNGG	8 cut(s)	256, 333, 377, 436, 789, 898, 1149, 1458
StyI	CCWWGG	4 cut(s)	339, 603, 629, 1596
TaaI	ACNGT	1 cut(s)	28
TaiI	ACGT	4 cut(s)	15, 595, 838, 883
TaqI	TCGA	6 cut(s)	294, 756, 913, 1239, 1290, 1694
TatI	WGTACW	1 cut(s)	712
TfiI	GAWTC	2 cut(s)	477, 994
Tru1I	TTAA	7 cut(s)	15, 519, 693, 920, 1140, 1214, 1494
Tru9I	TTAA	7 cut(s)	15, 519, 693, 920, 1140, 1214, 1494
TscAI	CASTG	4 cut(s)	592, 1017, 1265, 1486
TseFI	GTSAC	1 cut(s)	934
TseI	GCWGC	3 cut(s)	320, 1952, 2123
Tsp45I	GTSAC	1 cut(s)	934
TspDTI	ATGAA	2 cut(s)	635, 1173
TspRI	CASTG	4 cut(s)	592, 1017, 1265, 1486
Tth111I	GACNNNGTC	1 cut(s)	1181
VpaK11BI	GGWCC	1 cut(s)	859
XapI	RAATTY	7 cut(s)	188, 695, 818, 1224, 1583, 1669, 2087
XceI	RCATGY	1 cut(s)	1657
XcmI	CCANNNNNNNNNTGG	1 cut(s)	331
XmnI	GAANNNNTTC	2 cut(s)	819, 1386
XspI	CTAG	7 cut(s)	303, 353, 639, 681, 1272, 1430, 1856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.