Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0008978

Physical Location & Seq

Genomic Coordinates Reverse (-)

51032866 .. 51038066

Gene Structure 5201 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG257600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1887 bp

ATGGGTAGCAACGCGAAGGGAAGGACTGTGCTTGAGGTTGGCGCTGATGGGGTGGCTCTCATCACCATCATCAACCCTCCTGTTAATTCCCTCTCCTTTGATGTGTTATTTGGCTTGAAAGAGAGCTATGAGGAGGCTCTGAGGAGAGAAGATGTCAAGGCAATTGTCGTTACAGGTGCAAGAGGGAAATTCTCTGGCGGATTTGATATTACTGCTTTTGGTGGACTTCAAGGAGGGCACAAAGCGGAGCCGAAGCCTGGTTATATATCTGTGGAGATTATCACAAACAGTGTAGAAGGTGCTAGGAAACCTTCAGTTGCTGCCATTGATGGCCTAGCATTGGGGGGAGGATTAGAGGTTGCAATGGCTTGCCATGCTCGAATATCAACTCCGACTGCCCAATTAGGCTTGCCGGAACTTCAGCTTGGACTCATCCCTGGATTTGGAGGAACGCAACGGCTTCCGCGTCTTGTTGGAATTTCAAAGGCACTTGAAATGATGCTGACTTCAAAACCGGTTAAAGGGGAAGAGGCTCATGATTTAGGCCTTGTTGATGCTATAGTTTCACCTGATGAGTTGGTAAGCACTGCACGTCGGTGGGCCTTGGATATCTTGGAGCGTCGGAGACCATGGGTTGCTAGTCTTCATAAGACTGACAAGTTAGAACCCCTTGGCGAAGCTAGGGAAATACTTAAATTTGCAAGAGCTCAAGTACGGAAGCAGGCTCCCAATCTCAAACATCCATTGGTTTGCATTGATGTTGTTGAAGAGGGTATAGCTTCTGGTGGCCGGGCTGGACTTTGGAAGGAAGCTCAAGAATTTCAAGGACTTCTACGTGCTGACACTTGCAAGAGCTTGGTCCACATCTTCTTTGCTCAACGTGGAACATCAAAGGTACCTGGGGTTACTGATCAAGGTTTGGTGCCTAGACGAGTGACTAAGGTTGCTGTTATTGGTGGAGGCTTGATGGGTTCTGGAATAACTACAGCGTTGATTCTTAGTAATTATCCAGTGATCCTGAAAGAAGTAAATGAGAAGTTCTTGCAGGCTGGGATTGGCAGAGTCAAAGCCAATCTGCAGAGCCGCGTCAAAAAAGGGAAAATGACTGAAGAGAAGTTTGAGAAGACACTCTCTCTTCTTGAAGGTACCCTGGACTATGAAAGCTTTAGAGATGTTGACATGGTCATAGAGGCAGTTATTGAGAAGGTTTCTTTAAAGCAACAAATTTTTGCTGACCTTGAAAAATATTGCCCACAACACTGCATACTTGCTAGTAACACTTCCACAATTGACTTGAATCTTATTGGAGATAAGACCAAATCCCATGATCGAATTATTGGAGCCCATTTCTTTAGTCCAGCACATGTCATGCCACTTTTGGAGATTGTTCGCACTAATCAGACATCTCCACAAGTTATTGTTGACTTGCTAGAAGTTGGGAAGAAGATAAAGAAAACCCCAATTGTTGTTGGTAACTGCACTGGATTTGCTGTTAACAGAACATTCTTTCCTTATGCCCAAGCTGGTCTTCTGCTTATTGAGCGTGGTGCAGATGTATATCAGATTGACAGGGCAATCACCAAATTTGGAATGCCCATGGGCCCATTCAGATTGGCTGATCTGGTTGGCTTTGATGTGGCGATTGCAACTGGCATGCAATATATTGAGAATTTTCCTGAGCGGACATATAAGTCGATGCTTATTCCTATGATGCAGGAGGATGGGAGAGCAGGTGAAACTACGAAAAGAGGGTTTTATGTTTATGATGATAAACGCAAAGCTAAGCCAGATCCTGAATTGAAAAAATACATTGAGAAGTCAAGGAGCATTTCTGGTCTTTCAATAGATGCCAAGGTATCTCTCTTAGATTTAGTCTGCACACCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004165 GO:0004300 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009062 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

628

Amino Acids

68.36

Weight (kDa)

8.95

Isoelectric Point (pI)

37.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	16 - 216	1.6e-40	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	28 - 195	7.3e-23	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	314 - 492	1.6e-61	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	495 - 588	7.8e-22	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008978)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06860
fragaria_vesca	FvH4_4g18500 FvH4_4g18500 FvH4_4g18500
malus_domestica	MD13G1166700.v1.1 MD16G1166400.v1.1
prunus_persica	Prupe.1G133000_v2.0.a1
pyrus_communis	pycom13g14220 pycom16g14060
rosa_chinensis	RchiOBHm_Chr4g0423051
rosa_laevigata	RLG00000007590
rosa_multiflora	Rmu_co8121856.1_g000001 Rmu_sc0006639.1_g000001 Rmu_sc0023965.1_g000001 Rmu_sc0028464.1_g000006
rosa_roxburghii	Rroxscaffold_5G00365130
rosa_rugosa	Rorug04G0183300.1
rosa_samantha	Rh4AG243100 Rh4BG247800 Rh4CG257600 Rh4DG242800
rosa_wichuraiana	Rw4G020970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1721
AasI	GACNNNNNNGTC	1 cut(s)	1690
Acc36I	ACCTGC	1 cut(s)	1721
Acc65I	GGTACC	2 cut(s)	895, 1145
AccB1I	GGYRCC	3 cut(s)	895, 922, 1145
AccBSI	CCGCTC	1 cut(s)	1681
AccII	CGCG	3 cut(s)	14, 466, 1086
AciI	CCGC	5 cut(s)	198, 245, 464, 1084, 1681
AclWI	GGATC	2 cut(s)	1009, 1784
AcoI	YGGCCR	1 cut(s)	787
AcsI	RAATTY	7 cut(s)	188, 477, 695, 818, 1224, 1583, 1669
AcuI	CTGAAG	3 cut(s)	297, 404, 1128
AfaI	GTAC	3 cut(s)	714, 897, 1147
AfiI	CCNNNNNNNGG	4 cut(s)	257, 340, 443, 521
AflIII	ACRYGT	1 cut(s)	1363
AgeI	ACCGGT	1 cut(s)	514
AjiI	CACGTC	1 cut(s)	593
AjnI	CCWGG	4 cut(s)	256, 436, 898, 1149
AjuI	GAANNNNNNNTTGG	4 cut(s)	408, 440, 1512, 1544
AleI	CACNNNNGTG	1 cut(s)	595
AloI	GAACNNNNNNTCC	2 cut(s)	1492, 1524
Alw21I	GWGCWC	1 cut(s)	709
Alw26I	GTCTC	1 cut(s)	619
AlwI	GGATC	2 cut(s)	1009, 1784
AlwNI	CAGNNNCTG	2 cut(s)	320, 1793
AoxI	GGCC	5 cut(s)	331, 544, 600, 787, 1600
ApaI	GGGCCC	1 cut(s)	1604
ApeKI	GCWGC	1 cut(s)	320
ApoI	RAATTY	7 cut(s)	188, 477, 695, 818, 1224, 1583, 1669
AsiGI	ACCGGT	1 cut(s)	514
Asp718I	GGTACC	2 cut(s)	895, 1145
AspLEI	GCGC	1 cut(s)	44
AspS9I	GGNCC	4 cut(s)	600, 859, 1600, 1601
AsuC2I	CCSGG	1 cut(s)	791
AsuHPI	GGTGA	4 cut(s)	55, 558, 1570, 1745
AvaII	GGWCC	1 cut(s)	859
BaeGI	GKGCMC	2 cut(s)	240, 1604
BanI	GGYRCC	3 cut(s)	895, 922, 1145
BanII	GRGCYC	3 cut(s)	709, 1345, 1604
BbsI	GAAGAC	3 cut(s)	635, 1130, 1520
Bbv12I	GWGCWC	1 cut(s)	709
BbvI	GCAGC	1 cut(s)	307
BccI	CCATC	5 cut(s)	41, 74, 323, 961, 1715
BceAI	ACGGC	1 cut(s)	473
BciT130I	CCWGG	4 cut(s)	258, 438, 900, 1151
BclI	TGATCA	1 cut(s)	910
BcnI	CCSGG	1 cut(s)	791
BcoDI	GTCTC	1 cut(s)	619
BfaI	CTAG	8 cut(s)	303, 335, 639, 681, 927, 1272, 1430, 1885
BfmI	CTRYAG	3 cut(s)	558, 984, 1076
BfoI	RGCGCY	1 cut(s)	45
BfuAI	ACCTGC	1 cut(s)	1721
BisI	GCNGC	2 cut(s)	321, 1084
BlpI	GCTNAGC	1 cut(s)	1782
BlsI	GCNGC	2 cut(s)	322, 1085
Bme1390I	CCNGG	5 cut(s)	258, 438, 791, 900, 1151
Bme18I	GGWCC	1 cut(s)	859
BmgBI	CACGTC	1 cut(s)	593
BmgT120I	GGNCC	4 cut(s)	600, 859, 1600, 1601
BmiI	GGNNCC	7 cut(s)	249, 726, 897, 924, 1147, 1342, 1602
BmrFI	CCNGG	5 cut(s)	258, 438, 791, 900, 1151
BmsI	GCATC	5 cut(s)	489, 544, 1686, 1701, 1837
BpiI	GAAGAC	3 cut(s)	635, 1130, 1520
Bpu10I	CCTNAGC	1 cut(s)	1677
Bpu1102I	GCTNAGC	1 cut(s)	1782
BpuEI	CTTGAG	3 cut(s)	53, 693, 798
BpuMI	CCSGG	1 cut(s)	791
BsaAI	YACGTR	1 cut(s)	836
BsaBI	GATNNNNATC	1 cut(s)	1557
BsaI	GGTCTC	1 cut(s)	619
BsaJI	CCNNGG	8 cut(s)	436, 603, 629, 670, 899, 1149, 1596, 1851
BsaWI	WCCGGW	1 cut(s)	514
BsaXI	ACNNNNNCTCC	2 cut(s)	1717, 1747
Bsc4I	CCNNNNNNNGG	4 cut(s)	257, 340, 443, 521
Bse118I	RCCGGY	1 cut(s)	514
Bse1I	ACTGG	3 cut(s)	1010, 1486, 1654
Bse3DI	GCAATG	1 cut(s)	369
Bse8I	GATNNNNATC	1 cut(s)	1557
BseBI	CCWGG	4 cut(s)	258, 438, 900, 1151
BseDI	CCNNGG	8 cut(s)	436, 603, 629, 670, 899, 1149, 1596, 1851
BseGI	GGATG	3 cut(s)	432, 739, 1726
BseJI	GATNNNNATC	1 cut(s)	1557
BseLI	CCNNNNNNNGG	4 cut(s)	257, 340, 443, 521
BseMI	GCAATG	1 cut(s)	369
BseMII	CTCAG	2 cut(s)	131, 1668
BseNI	ACTGG	3 cut(s)	1010, 1486, 1654
BseRI	GAGGAG	2 cut(s)	146, 157
BseSI	GKGCMC	2 cut(s)	240, 1604
BseXI	GCAGC	1 cut(s)	307
BseYI	CCCAGC	1 cut(s)	1049
BsgI	GTGCAG	4 cut(s)	573, 1462, 1569, 1861
Bsh1236I	CGCG	3 cut(s)	14, 466, 1086
BshFI	GGCC	5 cut(s)	333, 546, 602, 789, 1602
BshNI	GGYRCC	3 cut(s)	895, 922, 1145
BshTI	ACCGGT	1 cut(s)	514
BsiHKAI	GWGCWC	1 cut(s)	709
BsiSI	CCGG	3 cut(s)	413, 515, 790
BslI	CCNNNNNNNGG	4 cut(s)	257, 340, 443, 521
BsmAI	GTCTC	1 cut(s)	619
BsmI	GAATGC	1 cut(s)	1596
BsnI	GGCC	5 cut(s)	333, 546, 602, 789, 1602
Bso31I	GGTCTC	1 cut(s)	619
Bsp120I	GGGCCC	1 cut(s)	1600
Bsp1286I	GDGCHC	4 cut(s)	240, 709, 1345, 1604
Bsp143I	GATC	5 cut(s)	910, 1014, 1327, 1618, 1789
Bsp1720I	GCTNAGC	1 cut(s)	1782
Bsp19I	CCATGG	2 cut(s)	629, 1596
BspACI	CCGC	5 cut(s)	198, 245, 464, 1084, 1681
BspANI	GGCC	5 cut(s)	333, 546, 602, 789, 1602
BspCNI	CTCAG	2 cut(s)	132, 1669
BspFNI	CGCG	3 cut(s)	14, 466, 1086
BspHI	TCATGA	1 cut(s)	535
BspLI	GGNNCC	7 cut(s)	249, 726, 897, 924, 1147, 1342, 1602
BspMAI	CTGCAG	1 cut(s)	1080
BspMI	ACCTGC	1 cut(s)	1721
BspPI	GGATC	2 cut(s)	1009, 1784
BspT107I	GGYRCC	3 cut(s)	895, 922, 1145
BspTNI	GGTCTC	1 cut(s)	619
BsrBI	CCGCTC	1 cut(s)	1681
BsrDI	GCAATG	1 cut(s)	369
BsrFI	RCCGGY	1 cut(s)	514
BsrI	ACTGG	3 cut(s)	1010, 1486, 1654
BssAI	RCCGGY	1 cut(s)	514
BssECI	CCNNGG	8 cut(s)	436, 603, 629, 670, 899, 1149, 1596, 1851
BssMI	GATC	5 cut(s)	910, 1014, 1327, 1618, 1789
BssT1I	CCWWGG	5 cut(s)	603, 629, 670, 1596, 1851
Bst2UI	CCWGG	4 cut(s)	258, 438, 900, 1151
Bst4CI	ACNGT	2 cut(s)	28, 290
Bst6I	CTCTTC	4 cut(s)	522, 762, 1104, 1140
BstBAI	YACGTR	1 cut(s)	836
BstC8I	GCNNGC	5 cut(s)	370, 410, 723, 1047, 1655
BstDEI	CTNAG	6 cut(s)	140, 939, 998, 1677, 1782, 1864
BstDSI	CCRYGG	2 cut(s)	629, 1596
BstF5I	GGATG	3 cut(s)	432, 739, 1726
BstFNI	CGCG	3 cut(s)	14, 466, 1086
BstH2I	RGCGCY	1 cut(s)	45
BstHHI	GCGC	1 cut(s)	44
BstKTI	GATC	5 cut(s)	913, 1017, 1330, 1621, 1792
BstMAI	GTCTC	1 cut(s)	619
BstMBI	GATC	5 cut(s)	910, 1014, 1327, 1618, 1789
BstMWI	GCNNNNNNNGC	2 cut(s)	374, 1540
BstNI	CCWGG	4 cut(s)	258, 438, 900, 1151
BstNSI	RCATGY	2 cut(s)	1367, 1657
BstSCI	CCNGG	5 cut(s)	256, 436, 789, 898, 1149
BstSFI	CTRYAG	3 cut(s)	558, 984, 1076
BstSLI	GKGCMC	2 cut(s)	240, 1604
BstUI	CGCG	3 cut(s)	14, 466, 1086
BstV1I	GCAGC	1 cut(s)	307
BstV2I	GAAGAC	3 cut(s)	635, 1130, 1520
BstX2I	RGATCY	1 cut(s)	1789
BstYI	RGATCY	1 cut(s)	1789
BsuRI	GGCC	5 cut(s)	333, 546, 602, 789, 1602
BtgI	CCRYGG	2 cut(s)	629, 1596
BtrI	CACGTC	1 cut(s)	593
BtsCI	GGATG	3 cut(s)	432, 739, 1726
BtsI	GCAGTG	2 cut(s)	585, 1258
BtsIMutI	CAGTG	5 cut(s)	295, 585, 1017, 1258, 1479
BveI	ACCTGC	1 cut(s)	1721
Cac8I	GCNNGC	5 cut(s)	370, 410, 723, 1047, 1655
CaiI	CAGNNNCTG	2 cut(s)	320, 1793
CciI	TCATGA	1 cut(s)	535
CfoI	GCGC	1 cut(s)	44
Cfr10I	RCCGGY	1 cut(s)	514
Cfr13I	GGNCC	4 cut(s)	600, 859, 1600, 1601
CseI	GACGC	3 cut(s)	455, 608, 1075
Csp6I	GTAC	3 cut(s)	713, 896, 1146
CspAI	ACCGGT	1 cut(s)	514
CviAII	CATG	9 cut(s)	374, 536, 630, 1180, 1325, 1364, 1369, 1597, 1654
CviQI	GTAC	3 cut(s)	713, 896, 1146
DdeI	CTNAG	6 cut(s)	140, 939, 998, 1677, 1782, 1864
DpnI	GATC	5 cut(s)	912, 1016, 1329, 1620, 1791
DpnII	GATC	5 cut(s)	910, 1014, 1327, 1618, 1789
DraI	TTTAAA	1 cut(s)	1215
DrdI	GACNNNNNNGTC	1 cut(s)	1690
DseDI	GACNNNNNNGTC	1 cut(s)	1690
EaeI	YGGCCR	1 cut(s)	787
Eam1104I	CTCTTC	4 cut(s)	522, 762, 1104, 1140
EarI	CTCTTC	4 cut(s)	522, 762, 1104, 1140
EciI	GGCGGA	1 cut(s)	213
Ecl136II	GAGCTC	1 cut(s)	707
Eco130I	CCWWGG	5 cut(s)	603, 629, 670, 1596, 1851
Eco147I	AGGCCT	1 cut(s)	546
Eco24I	GRGCYC	3 cut(s)	709, 1345, 1604
Eco31I	GGTCTC	1 cut(s)	619
Eco32I	GATATC	1 cut(s)	610
Eco47I	GGWCC	1 cut(s)	859
Eco53kI	GAGCTC	1 cut(s)	707
Eco57I	CTGAAG	3 cut(s)	297, 404, 1128
EcoICRI	GAGCTC	1 cut(s)	707
EcoRII	CCWGG	4 cut(s)	256, 436, 898, 1149
EcoRV	GATATC	1 cut(s)	610
EcoT14I	CCWWGG	5 cut(s)	603, 629, 670, 1596, 1851
EcoT38I	GRGCYC	3 cut(s)	709, 1345, 1604
ErhI	CCWWGG	5 cut(s)	603, 629, 670, 1596, 1851
FaeI	CATG	9 cut(s)	377, 539, 633, 1183, 1328, 1367, 1372, 1600, 1657
FalI	AAGNNNNNCTT	2 cut(s)	1512, 1544
FatI	CATG	9 cut(s)	373, 535, 629, 1179, 1324, 1363, 1368, 1596, 1653
FbaI	TGATCA	1 cut(s)	910
Fnu4HI	GCNGC	2 cut(s)	321, 1084
FokI	GGATG	3 cut(s)	419, 726, 1733
FriOI	GRGCYC	3 cut(s)	709, 1345, 1604
Fsp4HI	GCNGC	2 cut(s)	321, 1084
FspBI	CTAG	8 cut(s)	303, 335, 639, 681, 927, 1272, 1430, 1885
GlaI	GCGC	1 cut(s)	43
GluI	GCNGC	2 cut(s)	321, 1084
GsaI	CCCAGC	1 cut(s)	1053
HaeII	RGCGCY	1 cut(s)	45
HaeIII	GGCC	5 cut(s)	333, 546, 602, 789, 1602
HapII	CCGG	3 cut(s)	413, 515, 790
HgaI	GACGC	3 cut(s)	455, 608, 1075
HhaI	GCGC	1 cut(s)	44
Hin1II	CATG	9 cut(s)	377, 539, 633, 1183, 1328, 1367, 1372, 1600, 1657
Hin6I	GCGC	1 cut(s)	42
HinP1I	GCGC	1 cut(s)	42
HincII	GTYRAC	3 cut(s)	1177, 1423, 1495
HindII	GTYRAC	3 cut(s)	1177, 1423, 1495
HindIII	AAGCTT	1 cut(s)	1162
HinfI	GANTC	4 cut(s)	429, 994, 1062, 1297
HpaI	GTTAAC	1 cut(s)	1495
HpaII	CCGG	3 cut(s)	413, 515, 790
HphI	GGTGA	4 cut(s)	55, 558, 1570, 1745
Hpy166II	GTNNAC	5 cut(s)	224, 862, 1177, 1423, 1495
Hpy188I	TCNGA	6 cut(s)	141, 393, 624, 1401, 1563, 1610
Hpy188III	TCNNGA	7 cut(s)	536, 815, 975, 1018, 1139, 1676, 1793
Hpy8I	GTNNAC	5 cut(s)	224, 862, 1177, 1423, 1495
Hpy99I	CGWCG	2 cut(s)	597, 624
HpyAV	CCTTC	7 cut(s)	10, 15, 290, 321, 799, 1136, 1198
HpyCH4III	ACNGT	2 cut(s)	28, 290
HpyCH4IV	ACGT	3 cut(s)	592, 835, 880
HpyF10VI	GCNNNNNNNGC	2 cut(s)	374, 1540
HpyF3I	CTNAG	6 cut(s)	140, 939, 998, 1677, 1782, 1864
HpySE526I	ACGT	3 cut(s)	592, 835, 880
Hsp92II	CATG	9 cut(s)	377, 539, 633, 1183, 1328, 1367, 1372, 1600, 1657
HspAI	GCGC	1 cut(s)	42
KpnI	GGTACC	2 cut(s)	899, 1149
Ksp22I	TGATCA	1 cut(s)	910
KspAI	GTTAAC	1 cut(s)	1495
Kzo9I	GATC	5 cut(s)	910, 1014, 1327, 1618, 1789
LmnI	GCTCC	5 cut(s)	247, 616, 730, 1340, 1824
Lsp1109I	GCAGC	1 cut(s)	307
LweI	GCATC	5 cut(s)	489, 544, 1686, 1701, 1837
MaeI	CTAG	8 cut(s)	303, 335, 639, 681, 927, 1272, 1430, 1885
MaeII	ACGT	3 cut(s)	592, 835, 880
MaeIII	GTNAC	5 cut(s)	169, 904, 934, 1274, 1472
MalI	GATC	5 cut(s)	912, 1016, 1329, 1620, 1791
MbiI	CCGCTC	1 cut(s)	1681
MboI	GATC	5 cut(s)	910, 1014, 1327, 1618, 1789
MfeI	CAATTG	3 cut(s)	162, 1287, 1461
MflI	RGATCY	1 cut(s)	1789
MhlI	GDGCHC	4 cut(s)	240, 709, 1345, 1604
MlyI	GAGTC	2 cut(s)	423, 1071
MmeI	TCCRAC	3 cut(s)	416, 454, 602
MseI	TTAA	5 cut(s)	84, 519, 693, 1214, 1494
MslI	CAYNNNNRTG	1 cut(s)	595
MspI	CCGG	3 cut(s)	413, 515, 790
MspR9I	CCNGG	5 cut(s)	258, 438, 791, 900, 1151
MunI	CAATTG	3 cut(s)	162, 1287, 1461
Mva1269I	GAATGC	1 cut(s)	1596
MvaI	CCWGG	4 cut(s)	258, 438, 900, 1151
MvnI	CGCG	3 cut(s)	14, 466, 1086
MwoI	GCNNNNNNNGC	2 cut(s)	374, 1540
NciI	CCSGG	1 cut(s)	791
NcoI	CCATGG	2 cut(s)	629, 1596
NdeII	GATC	5 cut(s)	910, 1014, 1327, 1618, 1789
NlaIII	CATG	9 cut(s)	377, 539, 633, 1183, 1328, 1367, 1372, 1600, 1657
NlaIV	GGNNCC	7 cut(s)	249, 726, 897, 924, 1147, 1342, 1602
NmuCI	GTSAC	1 cut(s)	934
NspI	RCATGY	2 cut(s)	1367, 1657
OliI	CACNNNNGTG	1 cut(s)	595
PaeI	GCATGC	1 cut(s)	1657
PagI	TCATGA	1 cut(s)	535
PaqCI	CACCTGC	1 cut(s)	1721
PceI	AGGCCT	1 cut(s)	546
PciI	ACATGT	1 cut(s)	1363
PcsI	WCGNNNNNNNCGW	1 cut(s)	463
PctI	GAATGC	1 cut(s)	1596
PfeI	GAWTC	2 cut(s)	994, 1297
PflFI	GACNNNGTC	1 cut(s)	1181
PinAI	ACCGGT	1 cut(s)	514
PkrI	GCNGC	2 cut(s)	322, 1085
PleI	GAGTC	2 cut(s)	423, 1070
PpsI	GAGTC	2 cut(s)	423, 1070
Ppu21I	YACGTR	1 cut(s)	836
PscI	ACATGT	1 cut(s)	1363
Psp124BI	GAGCTC	1 cut(s)	709
Psp6I	CCWGG	4 cut(s)	256, 436, 898, 1149
PspFI	CCCAGC	1 cut(s)	1049
PspGI	CCWGG	4 cut(s)	256, 436, 898, 1149
PspN4I	GGNNCC	7 cut(s)	249, 726, 897, 924, 1147, 1342, 1602
PspOMI	GGGCCC	1 cut(s)	1600
PspPI	GGNCC	4 cut(s)	600, 859, 1600, 1601
PstI	CTGCAG	1 cut(s)	1080
PstNI	CAGNNNCTG	2 cut(s)	320, 1793
PsuI	RGATCY	1 cut(s)	1789
PsyI	GACNNNGTC	1 cut(s)	1181
RsaI	GTAC	3 cut(s)	714, 897, 1147
RsaNI	GTAC	3 cut(s)	713, 896, 1146
RseI	CAYNNNNRTG	1 cut(s)	595
SacI	GAGCTC	1 cut(s)	709
SaqAI	TTAA	5 cut(s)	84, 519, 693, 1214, 1494
SatI	GCNGC	2 cut(s)	321, 1084
Sau3AI	GATC	5 cut(s)	910, 1014, 1327, 1618, 1789
Sau96I	GGNCC	4 cut(s)	600, 859, 1600, 1601
SchI	GAGTC	2 cut(s)	423, 1071
ScrFI	CCNGG	5 cut(s)	258, 438, 791, 900, 1151
SduI	GDGCHC	4 cut(s)	240, 709, 1345, 1604
SfaNI	GCATC	5 cut(s)	489, 544, 1686, 1701, 1837
SfcI	CTRYAG	3 cut(s)	558, 984, 1076
SinI	GGWCC	1 cut(s)	859
SmiMI	CAYNNNNRTG	1 cut(s)	595
SmlI	CTYRAG	3 cut(s)	32, 708, 813
SmoI	CTYRAG	3 cut(s)	32, 708, 813
SphI	GCATGC	1 cut(s)	1657
SseBI	AGGCCT	1 cut(s)	546
SsiI	CCGC	5 cut(s)	198, 245, 464, 1084, 1681
SspI	AATATT	1 cut(s)	1247
SspMI	CTAG	8 cut(s)	303, 335, 639, 681, 927, 1272, 1430, 1885
SstI	GAGCTC	1 cut(s)	709
StuI	AGGCCT	1 cut(s)	546
StyD4I	CCNGG	5 cut(s)	256, 436, 789, 898, 1149
StyI	CCWWGG	5 cut(s)	603, 629, 670, 1596, 1851
TaaI	ACNGT	2 cut(s)	28, 290
TaiI	ACGT	3 cut(s)	595, 838, 883
TaqI	TCGA	3 cut(s)	379, 1330, 1694
TauI	GCSGC	1 cut(s)	1086
TfiI	GAWTC	2 cut(s)	994, 1297
Tru1I	TTAA	5 cut(s)	84, 519, 693, 1214, 1494
Tru9I	TTAA	5 cut(s)	84, 519, 693, 1214, 1494
TscAI	CASTG	5 cut(s)	295, 592, 1017, 1265, 1486
TseFI	GTSAC	1 cut(s)	934
TseI	GCWGC	1 cut(s)	320
Tsp45I	GTSAC	1 cut(s)	934
TspDTI	ATGAA	2 cut(s)	635, 1173
TspGWI	ACGGA	1 cut(s)	730
TspRI	CASTG	5 cut(s)	295, 592, 1017, 1265, 1486
Tth111I	GACNNNGTC	1 cut(s)	1181
VpaK11BI	GGWCC	1 cut(s)	859
XapI	RAATTY	7 cut(s)	188, 477, 695, 818, 1224, 1583, 1669
XceI	RCATGY	2 cut(s)	1367, 1657
XspI	CTAG	8 cut(s)	303, 335, 639, 681, 927, 1272, 1430, 1885

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.