Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0008978

Physical Location & Seq

Genomic Coordinates Reverse (-)

46451161 .. 46456091

Gene Structure 4931 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00365130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2202 bp

ATGGGTAGCAAAGCGAAGGGAAGGACTGTTCTTGAGGTTGGAGCTGATGGGGTGGCGCTCATCACCATCATCAACCCTCCTGTCAATTCCCTCTCCTTTGATGTGTTATTTGGCTTGAAAGAGAGCTATGAGGAGGCTCTGAGGAGAGAAGATGTCAAGGCAATTGTCGTTACGGGTGCAAAAGGGAAATTCTCTGGCGGATTTGATATTACTGCTTTTGGTGGACTTCAAGGAGGGCAAAAAGCGGAGCCGAAGCCTGGTTATATATCTGTGGAGGTTATCACTAACACTTTAGAAGGCTTGCCATGCTCGAATATCAACTCCGACTGCCCAATTAGGCTTGCCAGAACTTCAGCTTGGACTCATCCCTGGATTTGGAGGTTTTCTCAAATATTGCCTTCACATGGACTGGTGATGGGGGCTGTCTCATGCTCTGAACGCAAACGGCTTCCTCGTCTTGTTGGAATCTCAAAGGCACTTGAAATGATGCTGACTTCAAAACCCGTTAAAGGGGAAGAGGCTCATGATTTAGGCCTTGTTGATGCTATAGTTTCACCTAATGAGTTGGTAAGCACTGCACGTCGGTGGGCCTTGGATATCTTGGAGCGTCGGAGACCATGGGTTGCTAGTCTTCATAAGACTGACAAGTTAGAACCCCTTGGCGAAGCTAGGGAAATACTTAAATTTGCAAGAGCTCAAGTACGGAGGCAGGCTCCCAATCTCAAACATCCATTGGTTTGCATTGATGTTGTTGAAGAGGGTATAGCTTCTGGTGGCCGGGCTGGACTTTGGAAGGAAGCTCAAGAATTTCAAGGACTTCTGCGTGCTGACACTTGCAAGAGCTTGGTCCACATCTTTTTTGCTCAACGTGGAACACCAAAGGTACCTGGGGTTACTGATCGAGGTTTGGTGCCTAGACGAGTGACTAAGGTTGCTGTCATTGGTGGAGGCTTGATGGGCTCTGGAATAACTACAGCATTGATTCTTAGTAATTATCCAGTGGTCTTGAAAGAAGTAAATGAGAAGTTCTTGCAGGCTGGGATTGGTAGAGTCAAAGCCAATCTGCAGAGCCGTGTCAGAAAAGGGAAAATGACTGAAGAGAAGTTTGAGAAGACACTCTCTCTTCTTAAAGGTACCCTGGACTATGAAGGCTTTAGAGATGTTGACATGGTCATAGAGGCAGTTATTGAGAAGGTTTCTTTAAAGCAACAAATTTTTGCTGACCTTGAAAAATATTGCCCACAACACTGCATACTTGCTAGTAACACTTCCACAATTGACTTGAACCTTATTGGAGAGAAGACCAAATCCCATGATCGAATTGTTGGAGCCCATTTCTTTAGTCCAGCACATGTCATGCCACTTTTGGAAATTGTTCGCACTAATCAGACATCTCCACAAGTTATTGTTGATTTGCTAGAAGTTGGGAAGAAGATAAAGAAAACCCCAGTTGTTGTTGGGAACTGCACTGGATTTGCTGTTAACAGAATGTTCTTTCCTTATACCCAAGCTGGTCTTCTGCTTATTGAGCGTGGTGCAGATGTATATCAGATTGACAGGGCAATCACCAAATTTGGAATGCCCATGGGCCCATTCAGATTGGCTGATCTGGTTGGCTTTGGTGTGGCAATTGCAACTGGCATGCAATTTATTGAGAATTTTCCTGAGCGGACATATAAGTCGATGCTTATTCCTATGATGCAGGAGGATGGGAGAGCAGGTGAAACTACAAAAAGAGGGTTTTATGTTTATGATGATAAACGCAAAGCTAAGCCAGATCCTGAATTGAAGAAATACATTGAGAAGTCAAGAAGCATTTCTGGTCTTTCAATAGATGCCAAGCTAGTGAAGTTACCAGAAAAGGACATTGTGGAAATGATCTTTTTCCCTGTTGTGAATGAGGCCTGCAGAGTGTATGCTGAAGGTATTGCTGTAAAAGCTGCTGACCTTGATATTGCTTCAGTCATGGGCATGGGGTTTCCACCTTACAGGGGAGGTATCATGTTCTGGGCCGATTTACTTGGATCCAAATACATATATTCAAGACTGGAGCAATGGTCAAACATTTATGGTGAATTTTTCAAGCCTTGTGCCTATTTGGCTGATAGGGCTGCACAGGGAGCTCCTCTGGAACGTTTAAATAATGTTCGAGGAGATGGCGATTGTTGTTACAGTGATCGCTTGGAGAATAACTGCCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004165 GO:0004300 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009062 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

733

Amino Acids

80.99

Weight (kDa)

9.05

Isoelectric Point (pI)

33.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	16 - 85	9e-07	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	146 - 212	2.4e-09	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	310 - 488	1.7e-61	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	491 - 584	3e-21	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008978)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06860
fragaria_vesca	FvH4_4g18500 FvH4_4g18500 FvH4_4g18500
malus_domestica	MD13G1166700.v1.1 MD16G1166400.v1.1
prunus_persica	Prupe.1G133000_v2.0.a1
pyrus_communis	pycom13g14220 pycom16g14060
rosa_chinensis	RchiOBHm_Chr4g0423051
rosa_laevigata	RLG00000007590
rosa_multiflora	Rmu_co8121856.1_g000001 Rmu_sc0006639.1_g000001 Rmu_sc0023965.1_g000001 Rmu_sc0028464.1_g000006
rosa_roxburghii	Rroxscaffold_5G00365130
rosa_rugosa	Rorug04G0183300.1
rosa_samantha	Rh4AG243100 Rh4BG247800 Rh4CG257600 Rh4DG242800
rosa_wichuraiana	Rw4G020970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1709
AasI	GACNNNNNNGTC	1 cut(s)	1678
Acc36I	ACCTGC	1 cut(s)	1709
Acc65I	GGTACC	2 cut(s)	883, 1133
AccB1I	GGYRCC	3 cut(s)	883, 910, 1133
AccBSI	CCGCTC	1 cut(s)	1669
AciI	CCGC	3 cut(s)	198, 245, 1669
AclI	AACGTT	1 cut(s)	2134
AclWI	GGATC	3 cut(s)	1772, 2019, 2032
AcoI	YGGCCR	1 cut(s)	775
AcsI	RAATTY	7 cut(s)	188, 683, 806, 1212, 1571, 1657, 2075
AcuI	CTGAAG	4 cut(s)	336, 1116, 1941, 1944
AfaI	GTAC	3 cut(s)	702, 885, 1135
AfiI	CCNNNNNNNGG	6 cut(s)	257, 375, 404, 509, 510, 1991
AflIII	ACRYGT	1 cut(s)	1351
AjiI	CACGTC	1 cut(s)	581
AjnI	CCWGG	4 cut(s)	256, 368, 886, 1137
AjuI	GAANNNNNNNTTGG	4 cut(s)	340, 372, 1500, 1532
AleI	CACNNNNGTG	1 cut(s)	583
AloI	GAACNNNNNNTCC	2 cut(s)	12, 44
Alw21I	GWGCWC	2 cut(s)	697, 2125
Alw26I	GTCTC	2 cut(s)	430, 607
AlwI	GGATC	3 cut(s)	1772, 2019, 2032
AlwNI	CAGNNNCTG	1 cut(s)	1781
AoxI	GGCC	6 cut(s)	532, 588, 775, 1588, 1902, 2010
ApaI	GGGCCC	1 cut(s)	1592
ApeKI	GCWGC	2 cut(s)	1940, 2111
ApoI	RAATTY	7 cut(s)	188, 683, 806, 1212, 1571, 1657, 2075
Asp700I	GAANNNNTTC	2 cut(s)	1374, 1816
Asp718I	GGTACC	2 cut(s)	883, 1133
AspLEI	GCGC	1 cut(s)	58
AspS9I	GGNCC	5 cut(s)	588, 847, 1588, 1589, 2010
AsuC2I	CCSGG	1 cut(s)	779
AsuHPI	GGTGA	6 cut(s)	55, 424, 546, 1558, 1733, 2084
AvaII	GGWCC	1 cut(s)	847
BaeGI	GKGCMC	1 cut(s)	1592
BamHI	GGATCC	1 cut(s)	2024
BanI	GGYRCC	3 cut(s)	883, 910, 1133
BanII	GRGCYC	5 cut(s)	697, 962, 1333, 1592, 2125
BbsI	GAAGAC	4 cut(s)	623, 1118, 1307, 1508
Bbv12I	GWGCWC	2 cut(s)	697, 2125
BbvI	GCAGC	2 cut(s)	1927, 2098
BccI	CCATC	6 cut(s)	41, 74, 409, 949, 1703, 2150
BceAI	ACGGC	2 cut(s)	461, 1056
BciT130I	CCWGG	4 cut(s)	258, 370, 888, 1139
BcnI	CCSGG	1 cut(s)	779
BcoDI	GTCTC	2 cut(s)	430, 607
BfaI	CTAG	7 cut(s)	627, 669, 915, 1260, 1418, 1844, 2200
BfmI	CTRYAG	4 cut(s)	546, 972, 1064, 1906
BfoI	RGCGCY	1 cut(s)	59
BfuAI	ACCTGC	1 cut(s)	1709
BglI	GCCNNNNNGGC	1 cut(s)	2099
BisI	GCNGC	2 cut(s)	1941, 2112
BlpI	GCTNAGC	1 cut(s)	1770
BlsI	GCNGC	2 cut(s)	1942, 2113
Bme1390I	CCNGG	5 cut(s)	258, 370, 779, 888, 1139
Bme18I	GGWCC	1 cut(s)	847
BmgBI	CACGTC	1 cut(s)	581
BmgT120I	GGNCC	5 cut(s)	588, 847, 1588, 1589, 2010
BmiI	GGNNCC	8 cut(s)	249, 714, 885, 912, 1135, 1330, 1590, 2026
BmrFI	CCNGG	5 cut(s)	258, 370, 779, 888, 1139
BmrI	ACTGGG	1 cut(s)	1442
BmsI	GCATC	5 cut(s)	477, 532, 1674, 1689, 1825
BmuI	ACTGGG	1 cut(s)	1442
BpiI	GAAGAC	4 cut(s)	623, 1118, 1307, 1508
BplI	GAGNNNNNCTC	4 cut(s)	370, 402, 697, 729
BpmI	CTGGAG	1 cut(s)	2069
Bpu10I	CCTNAGC	1 cut(s)	1665
Bpu1102I	GCTNAGC	1 cut(s)	1770
BpuEI	CTTGAG	3 cut(s)	53, 681, 786
BpuMI	CCSGG	1 cut(s)	779
BsaBI	GATNNNNATC	1 cut(s)	1545
BsaI	GGTCTC	1 cut(s)	607
BsaJI	CCNNGG	7 cut(s)	368, 591, 617, 658, 887, 1137, 1584
BsaXI	ACNNNNNCTCC	4 cut(s)	1705, 1735, 2042, 2072
Bsc4I	CCNNNNNNNGG	6 cut(s)	257, 375, 404, 509, 510, 1991
Bse1I	ACTGG	6 cut(s)	414, 998, 1448, 1474, 1642, 2052
Bse3DI	GCAATG	1 cut(s)	2060
Bse8I	GATNNNNATC	1 cut(s)	1545
BseBI	CCWGG	4 cut(s)	258, 370, 888, 1139
BseDI	CCNNGG	7 cut(s)	368, 591, 617, 658, 887, 1137, 1584
BseGI	GGATG	3 cut(s)	364, 727, 1714
BseJI	GATNNNNATC	1 cut(s)	1545
BseLI	CCNNNNNNNGG	6 cut(s)	257, 375, 404, 509, 510, 1991
BseMI	GCAATG	1 cut(s)	2060
BseMII	CTCAG	2 cut(s)	131, 1656
BseNI	ACTGG	6 cut(s)	414, 998, 1448, 1474, 1642, 2052
BseRI	GAGGAG	4 cut(s)	146, 157, 2115, 2166
BseSI	GKGCMC	1 cut(s)	1592
BseXI	GCAGC	2 cut(s)	1927, 2098
BseYI	CCCAGC	1 cut(s)	1037
BsgI	GTGCAG	4 cut(s)	561, 1450, 1557, 2097
BshFI	GGCC	6 cut(s)	534, 590, 777, 1590, 1904, 2012
BshNI	GGYRCC	3 cut(s)	883, 910, 1133
BsiHKAI	GWGCWC	2 cut(s)	697, 2125
BsiSI	CCGG	1 cut(s)	778
BslI	CCNNNNNNNGG	6 cut(s)	257, 375, 404, 509, 510, 1991
BsmAI	GTCTC	2 cut(s)	430, 607
BsmI	GAATGC	1 cut(s)	1584
BsnI	GGCC	6 cut(s)	534, 590, 777, 1590, 1904, 2012
Bso31I	GGTCTC	1 cut(s)	607
Bsp120I	GGGCCC	1 cut(s)	1588
Bsp1286I	GDGCHC	5 cut(s)	697, 962, 1333, 1592, 2125
Bsp143I	GATC	7 cut(s)	898, 1315, 1606, 1777, 1878, 2024, 2176
Bsp1720I	GCTNAGC	1 cut(s)	1770
Bsp19I	CCATGG	2 cut(s)	617, 1584
BspACI	CCGC	3 cut(s)	198, 245, 1669
BspANI	GGCC	6 cut(s)	534, 590, 777, 1590, 1904, 2012
BspCNI	CTCAG	2 cut(s)	132, 1657
BspHI	TCATGA	1 cut(s)	523
BspLI	GGNNCC	8 cut(s)	249, 714, 885, 912, 1135, 1330, 1590, 2026
BspMAI	CTGCAG	2 cut(s)	1068, 1910
BspMI	ACCTGC	1 cut(s)	1709
BspPI	GGATC	3 cut(s)	1772, 2019, 2032
BspT107I	GGYRCC	3 cut(s)	883, 910, 1133
BspTNI	GGTCTC	1 cut(s)	607
BsrBI	CCGCTC	1 cut(s)	1669
BsrDI	GCAATG	1 cut(s)	2060
BsrI	ACTGG	6 cut(s)	414, 998, 1448, 1474, 1642, 2052
BssECI	CCNNGG	7 cut(s)	368, 591, 617, 658, 887, 1137, 1584
BssMI	GATC	7 cut(s)	898, 1315, 1606, 1777, 1878, 2024, 2176
BssT1I	CCWWGG	4 cut(s)	591, 617, 658, 1584
Bst2UI	CCWGG	4 cut(s)	258, 370, 888, 1139
Bst4CI	ACNGT	2 cut(s)	28, 2174
Bst6I	CTCTTC	4 cut(s)	510, 750, 1092, 1128
BstC8I	GCNNGC	7 cut(s)	302, 342, 711, 825, 1035, 1643, 1906
BstDEI	CTNAG	5 cut(s)	140, 927, 986, 1665, 1770
BstDSI	CCRYGG	2 cut(s)	617, 1584
BstF5I	GGATG	3 cut(s)	364, 727, 1714
BstH2I	RGCGCY	1 cut(s)	59
BstHHI	GCGC	1 cut(s)	58
BstKTI	GATC	7 cut(s)	901, 1318, 1609, 1780, 1881, 2027, 2179
BstMAI	GTCTC	2 cut(s)	430, 607
BstMBI	GATC	7 cut(s)	898, 1315, 1606, 1777, 1878, 2024, 2176
BstMWI	GCNNNNNNNGC	8 cut(s)	306, 438, 957, 1528, 1937, 2099, 2108, 2120
BstNI	CCWGG	4 cut(s)	258, 370, 888, 1139
BstNSI	RCATGY	2 cut(s)	1355, 1645
BstSCI	CCNGG	5 cut(s)	256, 368, 777, 886, 1137
BstSFI	CTRYAG	4 cut(s)	546, 972, 1064, 1906
BstSLI	GKGCMC	1 cut(s)	1592
BstV1I	GCAGC	2 cut(s)	1927, 2098
BstV2I	GAAGAC	4 cut(s)	623, 1118, 1307, 1508
BstX2I	RGATCY	2 cut(s)	1777, 2024
BstYI	RGATCY	2 cut(s)	1777, 2024
BsuRI	GGCC	6 cut(s)	534, 590, 777, 1590, 1904, 2012
BtgI	CCRYGG	2 cut(s)	617, 1584
BtrI	CACGTC	1 cut(s)	581
BtsCI	GGATG	3 cut(s)	364, 727, 1714
BtsI	GCAGTG	2 cut(s)	573, 1246
BtsIMutI	CAGTG	5 cut(s)	573, 1005, 1246, 1467, 2179
BveI	ACCTGC	1 cut(s)	1709
Cac8I	GCNNGC	7 cut(s)	302, 342, 711, 825, 1035, 1643, 1906
CaiI	CAGNNNCTG	1 cut(s)	1781
CciI	TCATGA	1 cut(s)	523
CfoI	GCGC	1 cut(s)	58
Cfr13I	GGNCC	5 cut(s)	588, 847, 1588, 1589, 2010
CseI	GACGC	1 cut(s)	596
Csp6I	GTAC	3 cut(s)	701, 884, 1134
CviQI	GTAC	3 cut(s)	701, 884, 1134
DdeI	CTNAG	5 cut(s)	140, 927, 986, 1665, 1770
DpnI	GATC	7 cut(s)	900, 1317, 1608, 1779, 1880, 2026, 2178
DpnII	GATC	7 cut(s)	898, 1315, 1606, 1777, 1878, 2024, 2176
DraI	TTTAAA	2 cut(s)	1203, 2139
DrdI	GACNNNNNNGTC	1 cut(s)	1678
DseDI	GACNNNNNNGTC	1 cut(s)	1678
EaeI	YGGCCR	1 cut(s)	775
Eam1104I	CTCTTC	4 cut(s)	510, 750, 1092, 1128
EarI	CTCTTC	4 cut(s)	510, 750, 1092, 1128
EciI	GGCGGA	1 cut(s)	213
Ecl136II	GAGCTC	2 cut(s)	695, 2123
Eco130I	CCWWGG	4 cut(s)	591, 617, 658, 1584
Eco147I	AGGCCT	2 cut(s)	534, 1904
Eco24I	GRGCYC	5 cut(s)	697, 962, 1333, 1592, 2125
Eco31I	GGTCTC	1 cut(s)	607
Eco32I	GATATC	1 cut(s)	598
Eco47I	GGWCC	1 cut(s)	847
Eco53kI	GAGCTC	2 cut(s)	695, 2123
Eco57I	CTGAAG	4 cut(s)	336, 1116, 1941, 1944
EcoICRI	GAGCTC	2 cut(s)	695, 2123
EcoRII	CCWGG	4 cut(s)	256, 368, 886, 1137
EcoRV	GATATC	1 cut(s)	598
EcoT14I	CCWWGG	4 cut(s)	591, 617, 658, 1584
EcoT38I	GRGCYC	5 cut(s)	697, 962, 1333, 1592, 2125
ErhI	CCWWGG	4 cut(s)	591, 617, 658, 1584
FalI	AAGNNNNNCTT	2 cut(s)	1500, 1532
Fnu4HI	GCNGC	2 cut(s)	1941, 2112
FokI	GGATG	3 cut(s)	351, 714, 1721
FriOI	GRGCYC	5 cut(s)	697, 962, 1333, 1592, 2125
Fsp4HI	GCNGC	2 cut(s)	1941, 2112
FspBI	CTAG	7 cut(s)	627, 669, 915, 1260, 1418, 1844, 2200
GlaI	GCGC	1 cut(s)	57
GluI	GCNGC	2 cut(s)	1941, 2112
GsaI	CCCAGC	1 cut(s)	1041
GsuI	CTGGAG	1 cut(s)	2069
HaeII	RGCGCY	1 cut(s)	59
HaeIII	GGCC	6 cut(s)	534, 590, 777, 1590, 1904, 2012
HapII	CCGG	1 cut(s)	778
HgaI	GACGC	1 cut(s)	596
HhaI	GCGC	1 cut(s)	58
Hin6I	GCGC	1 cut(s)	56
HinP1I	GCGC	1 cut(s)	56
HincII	GTYRAC	2 cut(s)	1165, 1483
HindII	GTYRAC	2 cut(s)	1165, 1483
HinfI	GANTC	4 cut(s)	361, 465, 982, 1050
HpaI	GTTAAC	1 cut(s)	1483
HpaII	CCGG	1 cut(s)	778
HphI	GGTGA	6 cut(s)	55, 424, 546, 1558, 1733, 2084
Hpy166II	GTNNAC	4 cut(s)	224, 850, 1165, 1483
Hpy188I	TCNGA	8 cut(s)	141, 325, 436, 612, 1079, 1389, 1551, 1598
Hpy8I	GTNNAC	4 cut(s)	224, 850, 1165, 1483
Hpy99I	CGWCG	2 cut(s)	585, 612
HpyAV	CCTTC	8 cut(s)	10, 15, 290, 408, 787, 1142, 1186, 1916
HpyCH4III	ACNGT	2 cut(s)	28, 2174
HpyCH4IV	ACGT	3 cut(s)	580, 868, 2134
HpyF10VI	GCNNNNNNNGC	8 cut(s)	306, 438, 957, 1528, 1937, 2099, 2108, 2120
HpyF3I	CTNAG	5 cut(s)	140, 927, 986, 1665, 1770
HpySE526I	ACGT	3 cut(s)	580, 868, 2134
HspAI	GCGC	1 cut(s)	56
KpnI	GGTACC	2 cut(s)	887, 1137
KspAI	GTTAAC	1 cut(s)	1483
Kzo9I	GATC	7 cut(s)	898, 1315, 1606, 1777, 1878, 2024, 2176
LmnI	GCTCC	8 cut(s)	41, 247, 604, 718, 1328, 2050, 2120, 2128
Lsp1109I	GCAGC	2 cut(s)	1927, 2098
LweI	GCATC	5 cut(s)	477, 532, 1674, 1689, 1825
MaeI	CTAG	7 cut(s)	627, 669, 915, 1260, 1418, 1844, 2200
MaeII	ACGT	3 cut(s)	580, 868, 2134
MaeIII	GTNAC	6 cut(s)	169, 892, 922, 1262, 1851, 2168
MalI	GATC	7 cut(s)	900, 1317, 1608, 1779, 1880, 2026, 2178
MbiI	CCGCTC	1 cut(s)	1669
MboI	GATC	7 cut(s)	898, 1315, 1606, 1777, 1878, 2024, 2176
MfeI	CAATTG	3 cut(s)	162, 1275, 1629
MflI	RGATCY	2 cut(s)	1777, 2024
MhlI	GDGCHC	5 cut(s)	697, 962, 1333, 1592, 2125
MlyI	GAGTC	2 cut(s)	355, 1059
MmeI	TCCRAC	5 cut(s)	19, 348, 442, 590, 1306
MroXI	GAANNNNTTC	2 cut(s)	1374, 1816
MseI	TTAA	6 cut(s)	507, 681, 1128, 1202, 1482, 2138
MslI	CAYNNNNRTG	2 cut(s)	583, 1970
MspI	CCGG	1 cut(s)	778
MspR9I	CCNGG	5 cut(s)	258, 370, 779, 888, 1139
MunI	CAATTG	3 cut(s)	162, 1275, 1629
Mva1269I	GAATGC	1 cut(s)	1584
MvaI	CCWGG	4 cut(s)	258, 370, 888, 1139
MwoI	GCNNNNNNNGC	8 cut(s)	306, 438, 957, 1528, 1937, 2099, 2108, 2120
NciI	CCSGG	1 cut(s)	779
NcoI	CCATGG	2 cut(s)	617, 1584
NdeII	GATC	7 cut(s)	898, 1315, 1606, 1777, 1878, 2024, 2176
NlaIV	GGNNCC	8 cut(s)	249, 714, 885, 912, 1135, 1330, 1590, 2026
NmuCI	GTSAC	1 cut(s)	922
NspI	RCATGY	2 cut(s)	1355, 1645
OliI	CACNNNNGTG	1 cut(s)	583
PaeI	GCATGC	1 cut(s)	1645
PagI	TCATGA	1 cut(s)	523
PaqCI	CACCTGC	1 cut(s)	1709
PceI	AGGCCT	2 cut(s)	534, 1904
PciI	ACATGT	1 cut(s)	1351
PcsI	WCGNNNNNNNCGW	1 cut(s)	451
PctI	GAATGC	1 cut(s)	1584
PdmI	GAANNNNTTC	2 cut(s)	1374, 1816
PfeI	GAWTC	2 cut(s)	465, 982
PflFI	GACNNNGTC	1 cut(s)	1169
PkrI	GCNGC	2 cut(s)	1942, 2113
PleI	GAGTC	2 cut(s)	355, 1058
PpsI	GAGTC	2 cut(s)	355, 1058
PscI	ACATGT	1 cut(s)	1351
Psp124BI	GAGCTC	2 cut(s)	697, 2125
Psp1406I	AACGTT	1 cut(s)	2134
Psp6I	CCWGG	4 cut(s)	256, 368, 886, 1137
PspFI	CCCAGC	1 cut(s)	1037
PspGI	CCWGG	4 cut(s)	256, 368, 886, 1137
PspN4I	GGNNCC	8 cut(s)	249, 714, 885, 912, 1135, 1330, 1590, 2026
PspOMI	GGGCCC	1 cut(s)	1588
PspPI	GGNCC	5 cut(s)	588, 847, 1588, 1589, 2010
PstI	CTGCAG	2 cut(s)	1068, 1910
PstNI	CAGNNNCTG	1 cut(s)	1781
PsuI	RGATCY	2 cut(s)	1777, 2024
PsyI	GACNNNGTC	1 cut(s)	1169
RsaI	GTAC	3 cut(s)	702, 885, 1135
RsaNI	GTAC	3 cut(s)	701, 884, 1134
RseI	CAYNNNNRTG	2 cut(s)	583, 1970
SacI	GAGCTC	2 cut(s)	697, 2125
SaqAI	TTAA	6 cut(s)	507, 681, 1128, 1202, 1482, 2138
SatI	GCNGC	2 cut(s)	1941, 2112
Sau3AI	GATC	7 cut(s)	898, 1315, 1606, 1777, 1878, 2024, 2176
Sau96I	GGNCC	5 cut(s)	588, 847, 1588, 1589, 2010
SchI	GAGTC	2 cut(s)	355, 1059
ScrFI	CCNGG	5 cut(s)	258, 370, 779, 888, 1139
SduI	GDGCHC	5 cut(s)	697, 962, 1333, 1592, 2125
SfaNI	GCATC	5 cut(s)	477, 532, 1674, 1689, 1825
SfcI	CTRYAG	4 cut(s)	546, 972, 1064, 1906
SinI	GGWCC	1 cut(s)	847
SmiMI	CAYNNNNRTG	2 cut(s)	583, 1970
SmlI	CTYRAG	3 cut(s)	32, 696, 801
SmoI	CTYRAG	3 cut(s)	32, 696, 801
SphI	GCATGC	1 cut(s)	1645
SseBI	AGGCCT	2 cut(s)	534, 1904
SsiI	CCGC	3 cut(s)	198, 245, 1669
SspI	AATATT	2 cut(s)	393, 1235
SspMI	CTAG	7 cut(s)	627, 669, 915, 1260, 1418, 1844, 2200
SstI	GAGCTC	2 cut(s)	697, 2125
StuI	AGGCCT	2 cut(s)	534, 1904
StyD4I	CCNGG	5 cut(s)	256, 368, 777, 886, 1137
StyI	CCWWGG	4 cut(s)	591, 617, 658, 1584
TaaI	ACNGT	2 cut(s)	28, 2174
TaiI	ACGT	3 cut(s)	583, 871, 2137
TaqI	TCGA	5 cut(s)	311, 901, 1318, 1682, 2149
TfiI	GAWTC	2 cut(s)	465, 982
Tru1I	TTAA	6 cut(s)	507, 681, 1128, 1202, 1482, 2138
Tru9I	TTAA	6 cut(s)	507, 681, 1128, 1202, 1482, 2138
TscAI	CASTG	5 cut(s)	580, 1005, 1253, 1474, 2179
TseFI	GTSAC	1 cut(s)	922
TseI	GCWGC	2 cut(s)	1940, 2111
Tsp45I	GTSAC	1 cut(s)	922
TspDTI	ATGAA	2 cut(s)	623, 1161
TspGWI	ACGGA	1 cut(s)	718
TspRI	CASTG	5 cut(s)	580, 1005, 1253, 1474, 2179
Tth111I	GACNNNGTC	1 cut(s)	1169
VpaK11BI	GGWCC	1 cut(s)	847
XapI	RAATTY	7 cut(s)	188, 683, 806, 1212, 1571, 1657, 2075
XceI	RCATGY	2 cut(s)	1355, 1645
XmnI	GAANNNNTTC	2 cut(s)	1374, 1816
XspI	CTAG	7 cut(s)	627, 669, 915, 1260, 1418, 1844, 2200

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.