Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0008978

Physical Location & Seq

Genomic Coordinates Forward (+)

18746404 .. 18751844

Gene Structure 5441 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000007590

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGGGTAGCAAAGCGAAGGGAAGGACTGTTCTTGAGGTTGGAGCTGATGGGGTGGCGCTCATCACCATCATCAACCCTCCCGTCAACTCCCTCTCCTTTGATGTGTTATTTGGCTTGAAAGAGAGCTACGAGGAGGCTCTGAGGAGAGAAGATGTCAAGGCAATTGTCGTTACAGGTGCAAAAGGGAAATTCTCTGGTGGATTTGATATTACTGCTTTTGGTGGACTTCAAGGAGGGCAGAAAGCGGAGCCGAAGCCTGGTTACATATCTGTGGAGGTTATCACTAACGGTGTAGAAGGTGCTAGGAAACCTTCAGTTGCTGCCATTGATGGCCTAGCATTGGGGGGAGGATTAGAGGTTGCAATGGCTTGCCATGCTCGAATATCAACTCCGACTGCCCAATTAGGCTTGCCAGAACTTCAGCTTGGACTCATCCCTGGATTTGGAGGAACGCAACGGCTTCCGCGTCTTGTTGGAATCTCAAAGGCACTTGAAATGATGCTGACTTCAAAACCAGTTAAAGGGGAAGAGGCTCATGATTTAGGCCTTGTTGATGCCATAGTTTCACCTGATGAGTTGGTAAGCACTGCACGTCGGTGGGCCTTGGATATCTTGGAGCGTCGGAGACCATGGGTTGCTAGTCTTCATAAGACTGACAAGTTAGAACCCCTTGGCGAAGCTAGGGAAATACTTAAATTTGCAAGAGCTCAAGTACGGAGGCAGGCTCCCAATCTCAAACATCCATTGGTTTGCATTGATGTTGTTGAAGAGGGGATAGCTTCTGGTGGCCGGGCTGGACTTTGGAAGGAAGCTCAAGAATTTCAAGGACTTCTGCGTGCTGACACTTGCAAGAGCTTGGTCCACATCTTCTTTGCTCAACGTGGAACATCAAAGGTACCTGGGGTTACTGATCGAGGTTTGGTGCCTAGACGAGTGACTAAGGTTGCTGTCATTGGTGGAGGCTTGATGGGCTCTGGAATAACTACAGCATTGATTCTTAGTAATTATCCAGTGATCCTGAAAGAAGTAAATGAGAAGTTCTTGCAGGCTGGGATTGGTAGAGTCAAAGCCAATCTGCAGAGCCGCGTCAAAAAAGGGAAAATGACTGAAGAGAAGTTTGAGAAGACACTCTCTCTTCTTAAAGGTACCCTGGACTATGAAAGCTTTAGAGATGTTGACATGGTCATAGAGGCAGTTATTGAGAAGGTTTCTTTAAAGCAACAAATTTTTGCTGACCTTGAAAAATATTGCCCACAACACTGCATACTTGCTAGTAACACTTCTACAATTGACTTGAACCTTATTGGAGAGAAGACCAAATCCCATGATCGAATTATTGGAGCCCATTTCTTTAGTCCAGCACATGTCATGCCACTTTTGGAAATTGTTCGCACTAATCAGACATCTCCACAAGTTATTGTTGATTTGCTAGAAGTTGGGAAGAAGATAAAGAAAACCCCAGTTGTTGTTGGGAACTGCACTGGATTTGCTGTTAACAGAATGTTCTTTCCTTATACCCAAGCTGGTCTTCTGCTTATTGAGCGTGGTGCAGATGTATATCAGATTGACAGGGCAATCACCAAATTTGGAATGCCTATGGGCCCATTCAGATTGGCTGATCTGGTTGGCTTTGGTGTGGCGATTGCAACTGGCATGCAATTTATTGAGAATTTTCCTGAGCGGACATATAAGTCGATGCTTATTCCTATGATGCAGGAGGATGGGAGAGTAGGTGAAACTACAAAAAGAGGGTTTTATGTTTATGATGATAAACGCAAAGCTAAGCCAGATCCTGAATTGAAGAAATACATTGAGAAGTCAAGAAGCATTTCTGGTCTTTCAATAGATGCCAAGCTAGTGAAGTTACCAGAAAAGGACATTGTGGAAATGATTTTTTTCCCTGTTGTGAATGAGGCCTGCAGAGTGTATGTTGAAGGTATTGCTGTAAAAGCTGCTGACCTTGATATTGCTTCAGTCATGGGCATGGGGTTTCCACCTTACAGGGGAGGTATCATGTTCTGGGCCGATTCACTTGGATCCAAATACATATATTCAAGACTGGAGCAATGGTCAAACATTTATGGTGAATTTTTCAAGCCTTGTGCCTATTTGGCTGAAAGGGCTGCACAGGGAGCTCCTCTGAGCTCTCCTTTGAAGCAATCAAAATCTCGGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004165 GO:0004300 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009062 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

726

Amino Acids

79.24

Weight (kDa)

9.21

Isoelectric Point (pI)

35.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	16 - 216	3.1e-40	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	28 - 195	1.2e-22	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	314 - 492	6.6e-62	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	495 - 588	2.6e-21	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008978)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06860
fragaria_vesca	FvH4_4g18500 FvH4_4g18500 FvH4_4g18500
malus_domestica	MD13G1166700.v1.1 MD16G1166400.v1.1
prunus_persica	Prupe.1G133000_v2.0.a1
pyrus_communis	pycom13g14220 pycom16g14060
rosa_chinensis	RchiOBHm_Chr4g0423051
rosa_laevigata	RLG00000007590
rosa_multiflora	Rmu_co8121856.1_g000001 Rmu_sc0006639.1_g000001 Rmu_sc0023965.1_g000001 Rmu_sc0028464.1_g000006
rosa_roxburghii	Rroxscaffold_5G00365130
rosa_rugosa	Rorug04G0183300.1
rosa_samantha	Rh4AG243100 Rh4BG247800 Rh4CG257600 Rh4DG242800
rosa_wichuraiana	Rw4G020970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1690
Acc65I	GGTACC	2 cut(s)	895, 1145
AccB1I	GGYRCC	3 cut(s)	895, 922, 1145
AccBSI	CCGCTC	1 cut(s)	1681
AccII	CGCG	2 cut(s)	466, 1086
AciI	CCGC	4 cut(s)	245, 464, 1084, 1681
AclWI	GGATC	4 cut(s)	1009, 1784, 2031, 2044
AcoI	YGGCCR	1 cut(s)	787
AcsI	RAATTY	7 cut(s)	188, 695, 818, 1224, 1583, 1669, 2087
AcuI	CTGAAG	4 cut(s)	297, 404, 1128, 1956
AfaI	GTAC	3 cut(s)	714, 897, 1147
AfiI	CCNNNNNNNGG	5 cut(s)	257, 340, 443, 521, 2003
AflIII	ACRYGT	1 cut(s)	1363
AjiI	CACGTC	1 cut(s)	593
AjnI	CCWGG	4 cut(s)	256, 436, 898, 1149
AjuI	GAANNNNNNNTTGG	4 cut(s)	408, 440, 1512, 1544
AleI	CACNNNNGTG	1 cut(s)	595
AloI	GAACNNNNNNTCC	2 cut(s)	12, 44
Alw21I	GWGCWC	3 cut(s)	709, 2137, 2147
Alw26I	GTCTC	1 cut(s)	619
AlwI	GGATC	4 cut(s)	1009, 1784, 2031, 2044
AlwNI	CAGNNNCTG	2 cut(s)	320, 1793
AoxI	GGCC	7 cut(s)	331, 544, 600, 787, 1600, 1914, 2022
ApaI	GGGCCC	1 cut(s)	1604
ApeKI	GCWGC	3 cut(s)	320, 1952, 2123
ApoI	RAATTY	7 cut(s)	188, 695, 818, 1224, 1583, 1669, 2087
Asp700I	GAANNNNTTC	2 cut(s)	1386, 1828
Asp718I	GGTACC	2 cut(s)	895, 1145
AspLEI	GCGC	1 cut(s)	58
AspS9I	GGNCC	5 cut(s)	600, 859, 1600, 1601, 2022
AsuC2I	CCSGG	1 cut(s)	791
AsuHPI	GGTGA	5 cut(s)	55, 558, 1570, 1745, 2096
AvaII	GGWCC	1 cut(s)	859
BaeGI	GKGCMC	1 cut(s)	1604
BamHI	GGATCC	1 cut(s)	2036
BanI	GGYRCC	3 cut(s)	895, 922, 1145
BanII	GRGCYC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
BarI	GAAGNNNNNNTAC	2 cut(s)	245, 277
BbsI	GAAGAC	4 cut(s)	635, 1130, 1319, 1520
Bbv12I	GWGCWC	3 cut(s)	709, 2137, 2147
BbvI	GCAGC	3 cut(s)	307, 1939, 2110
BccI	CCATC	5 cut(s)	41, 74, 323, 961, 1715
BceAI	ACGGC	1 cut(s)	473
BciT130I	CCWGG	4 cut(s)	258, 438, 900, 1151
BcnI	CCSGG	1 cut(s)	791
BcoDI	GTCTC	1 cut(s)	619
BfaI	CTAG	8 cut(s)	303, 335, 639, 681, 927, 1272, 1430, 1856
BfmI	CTRYAG	3 cut(s)	984, 1076, 1918
BfoI	RGCGCY	1 cut(s)	59
BglI	GCCNNNNNGGC	1 cut(s)	2111
BisI	GCNGC	4 cut(s)	321, 1084, 1953, 2124
BlpI	GCTNAGC	1 cut(s)	1782
BlsI	GCNGC	4 cut(s)	322, 1085, 1954, 2125
Bme1390I	CCNGG	5 cut(s)	258, 438, 791, 900, 1151
Bme18I	GGWCC	1 cut(s)	859
BmgBI	CACGTC	1 cut(s)	593
BmgT120I	GGNCC	5 cut(s)	600, 859, 1600, 1601, 2022
BmiI	GGNNCC	8 cut(s)	249, 726, 897, 924, 1147, 1342, 1602, 2038
BmrFI	CCNGG	5 cut(s)	258, 438, 791, 900, 1151
BmrI	ACTGGG	1 cut(s)	1454
BmsI	GCATC	5 cut(s)	489, 544, 1686, 1701, 1837
BmuI	ACTGGG	1 cut(s)	1454
BpiI	GAAGAC	4 cut(s)	635, 1130, 1319, 1520
BplI	GAGNNNNNCTC	2 cut(s)	709, 741
BpmI	CTGGAG	1 cut(s)	2081
Bpu10I	CCTNAGC	1 cut(s)	1677
Bpu1102I	GCTNAGC	1 cut(s)	1782
BpuEI	CTTGAG	3 cut(s)	53, 693, 798
BpuMI	CCSGG	1 cut(s)	791
BsaBI	GATNNNNATC	1 cut(s)	1557
BsaI	GGTCTC	1 cut(s)	619
BsaJI	CCNNGG	6 cut(s)	436, 603, 629, 670, 899, 1149
BsaXI	ACNNNNNCTCC	6 cut(s)	77, 107, 1717, 1747, 2054, 2084
Bsc4I	CCNNNNNNNGG	5 cut(s)	257, 340, 443, 521, 2003
Bse1I	ACTGG	6 cut(s)	515, 1010, 1460, 1486, 1654, 2064
Bse3DI	GCAATG	2 cut(s)	369, 2072
Bse8I	GATNNNNATC	1 cut(s)	1557
BseBI	CCWGG	4 cut(s)	258, 438, 900, 1151
BseDI	CCNNGG	6 cut(s)	436, 603, 629, 670, 899, 1149
BseGI	GGATG	3 cut(s)	432, 739, 1726
BseJI	GATNNNNATC	1 cut(s)	1557
BseLI	CCNNNNNNNGG	5 cut(s)	257, 340, 443, 521, 2003
BseMI	GCAATG	2 cut(s)	369, 2072
BseMII	CTCAG	3 cut(s)	131, 1668, 2132
BseNI	ACTGG	6 cut(s)	515, 1010, 1460, 1486, 1654, 2064
BseRI	GAGGAG	3 cut(s)	146, 157, 2127
BseSI	GKGCMC	1 cut(s)	1604
BseXI	GCAGC	3 cut(s)	307, 1939, 2110
BseYI	CCCAGC	1 cut(s)	1049
BsgI	GTGCAG	4 cut(s)	573, 1462, 1569, 2109
Bsh1236I	CGCG	2 cut(s)	466, 1086
BshFI	GGCC	7 cut(s)	333, 546, 602, 789, 1602, 1916, 2024
BshNI	GGYRCC	3 cut(s)	895, 922, 1145
BsiHKAI	GWGCWC	3 cut(s)	709, 2137, 2147
BsiSI	CCGG	1 cut(s)	790
BslI	CCNNNNNNNGG	5 cut(s)	257, 340, 443, 521, 2003
BsmAI	GTCTC	1 cut(s)	619
BsmI	GAATGC	1 cut(s)	1596
BsnI	GGCC	7 cut(s)	333, 546, 602, 789, 1602, 1916, 2024
Bso31I	GGTCTC	1 cut(s)	619
Bsp120I	GGGCCC	1 cut(s)	1600
Bsp1286I	GDGCHC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
Bsp143I	GATC	6 cut(s)	910, 1014, 1327, 1618, 1789, 2036
Bsp1720I	GCTNAGC	1 cut(s)	1782
Bsp19I	CCATGG	1 cut(s)	629
BspACI	CCGC	4 cut(s)	245, 464, 1084, 1681
BspANI	GGCC	7 cut(s)	333, 546, 602, 789, 1602, 1916, 2024
BspCNI	CTCAG	3 cut(s)	132, 1669, 2133
BspFNI	CGCG	2 cut(s)	466, 1086
BspHI	TCATGA	1 cut(s)	535
BspLI	GGNNCC	8 cut(s)	249, 726, 897, 924, 1147, 1342, 1602, 2038
BspMAI	CTGCAG	2 cut(s)	1080, 1922
BspPI	GGATC	4 cut(s)	1009, 1784, 2031, 2044
BspT107I	GGYRCC	3 cut(s)	895, 922, 1145
BspTNI	GGTCTC	1 cut(s)	619
BsrBI	CCGCTC	1 cut(s)	1681
BsrDI	GCAATG	2 cut(s)	369, 2072
BsrI	ACTGG	6 cut(s)	515, 1010, 1460, 1486, 1654, 2064
BssECI	CCNNGG	6 cut(s)	436, 603, 629, 670, 899, 1149
BssMI	GATC	6 cut(s)	910, 1014, 1327, 1618, 1789, 2036
BssT1I	CCWWGG	3 cut(s)	603, 629, 670
Bst2UI	CCWGG	4 cut(s)	258, 438, 900, 1151
Bst4CI	ACNGT	2 cut(s)	28, 290
Bst6I	CTCTTC	4 cut(s)	522, 762, 1104, 1140
BstC8I	GCNNGC	7 cut(s)	370, 410, 723, 837, 1047, 1655, 1918
BstDEI	CTNAG	6 cut(s)	140, 939, 998, 1677, 1782, 2141
BstDSI	CCRYGG	1 cut(s)	629
BstF5I	GGATG	3 cut(s)	432, 739, 1726
BstFNI	CGCG	2 cut(s)	466, 1086
BstH2I	RGCGCY	1 cut(s)	59
BstHHI	GCGC	1 cut(s)	58
BstKTI	GATC	6 cut(s)	913, 1017, 1330, 1621, 1792, 2039
BstMAI	GTCTC	1 cut(s)	619
BstMBI	GATC	6 cut(s)	910, 1014, 1327, 1618, 1789, 2036
BstMWI	GCNNNNNNNGC	7 cut(s)	374, 969, 1540, 1949, 2111, 2120, 2132
BstNI	CCWGG	4 cut(s)	258, 438, 900, 1151
BstNSI	RCATGY	2 cut(s)	1367, 1657
BstSCI	CCNGG	5 cut(s)	256, 436, 789, 898, 1149
BstSFI	CTRYAG	3 cut(s)	984, 1076, 1918
BstSLI	GKGCMC	1 cut(s)	1604
BstUI	CGCG	2 cut(s)	466, 1086
BstV1I	GCAGC	3 cut(s)	307, 1939, 2110
BstV2I	GAAGAC	4 cut(s)	635, 1130, 1319, 1520
BstX2I	RGATCY	2 cut(s)	1789, 2036
BstYI	RGATCY	2 cut(s)	1789, 2036
BsuRI	GGCC	7 cut(s)	333, 546, 602, 789, 1602, 1916, 2024
BtgI	CCRYGG	1 cut(s)	629
BtrI	CACGTC	1 cut(s)	593
BtsCI	GGATG	3 cut(s)	432, 739, 1726
BtsI	GCAGTG	2 cut(s)	585, 1258
BtsIMutI	CAGTG	4 cut(s)	585, 1017, 1258, 1479
Cac8I	GCNNGC	7 cut(s)	370, 410, 723, 837, 1047, 1655, 1918
CaiI	CAGNNNCTG	2 cut(s)	320, 1793
CciI	TCATGA	1 cut(s)	535
CfoI	GCGC	1 cut(s)	58
Cfr13I	GGNCC	5 cut(s)	600, 859, 1600, 1601, 2022
CseI	GACGC	3 cut(s)	455, 608, 1075
Csp6I	GTAC	3 cut(s)	713, 896, 1146
CviQI	GTAC	3 cut(s)	713, 896, 1146
DdeI	CTNAG	6 cut(s)	140, 939, 998, 1677, 1782, 2141
DpnI	GATC	6 cut(s)	912, 1016, 1329, 1620, 1791, 2038
DpnII	GATC	6 cut(s)	910, 1014, 1327, 1618, 1789, 2036
DraI	TTTAAA	1 cut(s)	1215
DrdI	GACNNNNNNGTC	1 cut(s)	1690
DseDI	GACNNNNNNGTC	1 cut(s)	1690
EaeI	YGGCCR	1 cut(s)	787
Eam1104I	CTCTTC	4 cut(s)	522, 762, 1104, 1140
EarI	CTCTTC	4 cut(s)	522, 762, 1104, 1140
Ecl136II	GAGCTC	3 cut(s)	707, 2135, 2145
Eco130I	CCWWGG	3 cut(s)	603, 629, 670
Eco147I	AGGCCT	2 cut(s)	546, 1916
Eco24I	GRGCYC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
Eco31I	GGTCTC	1 cut(s)	619
Eco32I	GATATC	1 cut(s)	610
Eco47I	GGWCC	1 cut(s)	859
Eco53kI	GAGCTC	3 cut(s)	707, 2135, 2145
Eco57I	CTGAAG	4 cut(s)	297, 404, 1128, 1956
EcoICRI	GAGCTC	3 cut(s)	707, 2135, 2145
EcoRII	CCWGG	4 cut(s)	256, 436, 898, 1149
EcoRV	GATATC	1 cut(s)	610
EcoT14I	CCWWGG	3 cut(s)	603, 629, 670
EcoT38I	GRGCYC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
ErhI	CCWWGG	3 cut(s)	603, 629, 670
FalI	AAGNNNNNCTT	2 cut(s)	1512, 1544
Fnu4HI	GCNGC	4 cut(s)	321, 1084, 1953, 2124
FokI	GGATG	3 cut(s)	419, 726, 1733
FriOI	GRGCYC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
Fsp4HI	GCNGC	4 cut(s)	321, 1084, 1953, 2124
FspBI	CTAG	8 cut(s)	303, 335, 639, 681, 927, 1272, 1430, 1856
GlaI	GCGC	1 cut(s)	57
GluI	GCNGC	4 cut(s)	321, 1084, 1953, 2124
GsaI	CCCAGC	1 cut(s)	1053
GsuI	CTGGAG	1 cut(s)	2081
HaeII	RGCGCY	1 cut(s)	59
HaeIII	GGCC	7 cut(s)	333, 546, 602, 789, 1602, 1916, 2024
HapII	CCGG	1 cut(s)	790
HgaI	GACGC	3 cut(s)	455, 608, 1075
HhaI	GCGC	1 cut(s)	58
Hin6I	GCGC	1 cut(s)	56
HinP1I	GCGC	1 cut(s)	56
HincII	GTYRAC	3 cut(s)	85, 1177, 1495
HindII	GTYRAC	3 cut(s)	85, 1177, 1495
HindIII	AAGCTT	1 cut(s)	1162
HinfI	GANTC	5 cut(s)	429, 477, 994, 1062, 2027
HpaI	GTTAAC	1 cut(s)	1495
HpaII	CCGG	1 cut(s)	790
HphI	GGTGA	5 cut(s)	55, 558, 1570, 1745, 2096
Hpy166II	GTNNAC	5 cut(s)	85, 224, 862, 1177, 1495
Hpy188I	TCNGA	7 cut(s)	141, 393, 624, 1401, 1563, 1610, 2142
Hpy188III	TCNNGA	9 cut(s)	32, 536, 815, 975, 1018, 1676, 1793, 1821, 2055
Hpy8I	GTNNAC	5 cut(s)	85, 224, 862, 1177, 1495
Hpy99I	CGWCG	2 cut(s)	597, 624
HpyAV	CCTTC	7 cut(s)	10, 15, 290, 321, 799, 1198, 1928
HpyCH4III	ACNGT	2 cut(s)	28, 290
HpyCH4IV	ACGT	2 cut(s)	592, 880
HpyF10VI	GCNNNNNNNGC	7 cut(s)	374, 969, 1540, 1949, 2111, 2120, 2132
HpyF3I	CTNAG	6 cut(s)	140, 939, 998, 1677, 1782, 2141
HpySE526I	ACGT	2 cut(s)	592, 880
HspAI	GCGC	1 cut(s)	56
KpnI	GGTACC	2 cut(s)	899, 1149
KspAI	GTTAAC	1 cut(s)	1495
Kzo9I	GATC	6 cut(s)	910, 1014, 1327, 1618, 1789, 2036
LmnI	GCTCC	8 cut(s)	41, 247, 616, 730, 1340, 2062, 2132, 2140
Lsp1109I	GCAGC	3 cut(s)	307, 1939, 2110
LweI	GCATC	5 cut(s)	489, 544, 1686, 1701, 1837
MaeI	CTAG	8 cut(s)	303, 335, 639, 681, 927, 1272, 1430, 1856
MaeII	ACGT	2 cut(s)	592, 880
MaeIII	GTNAC	6 cut(s)	169, 260, 904, 934, 1274, 1863
MalI	GATC	6 cut(s)	912, 1016, 1329, 1620, 1791, 2038
MbiI	CCGCTC	1 cut(s)	1681
MboI	GATC	6 cut(s)	910, 1014, 1327, 1618, 1789, 2036
MfeI	CAATTG	2 cut(s)	162, 1287
MflI	RGATCY	2 cut(s)	1789, 2036
MhlI	GDGCHC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
MlyI	GAGTC	2 cut(s)	423, 1071
MmeI	TCCRAC	4 cut(s)	19, 416, 454, 602
MroXI	GAANNNNTTC	2 cut(s)	1386, 1828
MseI	TTAA	5 cut(s)	519, 693, 1140, 1214, 1494
MslI	CAYNNNNRTG	3 cut(s)	269, 595, 1982
MspI	CCGG	1 cut(s)	790
MspR9I	CCNGG	5 cut(s)	258, 438, 791, 900, 1151
MunI	CAATTG	2 cut(s)	162, 1287
Mva1269I	GAATGC	1 cut(s)	1596
MvaI	CCWGG	4 cut(s)	258, 438, 900, 1151
MvnI	CGCG	2 cut(s)	466, 1086
MwoI	GCNNNNNNNGC	7 cut(s)	374, 969, 1540, 1949, 2111, 2120, 2132
NciI	CCSGG	1 cut(s)	791
NcoI	CCATGG	1 cut(s)	629
NdeII	GATC	6 cut(s)	910, 1014, 1327, 1618, 1789, 2036
NlaIV	GGNNCC	8 cut(s)	249, 726, 897, 924, 1147, 1342, 1602, 2038
NmuCI	GTSAC	1 cut(s)	934
NspI	RCATGY	2 cut(s)	1367, 1657
OliI	CACNNNNGTG	1 cut(s)	595
PaeI	GCATGC	1 cut(s)	1657
PagI	TCATGA	1 cut(s)	535
PceI	AGGCCT	2 cut(s)	546, 1916
PciI	ACATGT	1 cut(s)	1363
PcsI	WCGNNNNNNNCGW	1 cut(s)	463
PctI	GAATGC	1 cut(s)	1596
PdmI	GAANNNNTTC	2 cut(s)	1386, 1828
PfeI	GAWTC	3 cut(s)	477, 994, 2027
PflFI	GACNNNGTC	1 cut(s)	1181
PkrI	GCNGC	4 cut(s)	322, 1085, 1954, 2125
PleI	GAGTC	2 cut(s)	423, 1070
PpsI	GAGTC	2 cut(s)	423, 1070
PscI	ACATGT	1 cut(s)	1363
Psp124BI	GAGCTC	3 cut(s)	709, 2137, 2147
Psp6I	CCWGG	4 cut(s)	256, 436, 898, 1149
PspFI	CCCAGC	1 cut(s)	1049
PspGI	CCWGG	4 cut(s)	256, 436, 898, 1149
PspN4I	GGNNCC	8 cut(s)	249, 726, 897, 924, 1147, 1342, 1602, 2038
PspOMI	GGGCCC	1 cut(s)	1600
PspPI	GGNCC	5 cut(s)	600, 859, 1600, 1601, 2022
PstI	CTGCAG	2 cut(s)	1080, 1922
PstNI	CAGNNNCTG	2 cut(s)	320, 1793
PsuI	RGATCY	2 cut(s)	1789, 2036
PsyI	GACNNNGTC	1 cut(s)	1181
RsaI	GTAC	3 cut(s)	714, 897, 1147
RsaNI	GTAC	3 cut(s)	713, 896, 1146
RseI	CAYNNNNRTG	3 cut(s)	269, 595, 1982
SacI	GAGCTC	3 cut(s)	709, 2137, 2147
SaqAI	TTAA	5 cut(s)	519, 693, 1140, 1214, 1494
SatI	GCNGC	4 cut(s)	321, 1084, 1953, 2124
Sau3AI	GATC	6 cut(s)	910, 1014, 1327, 1618, 1789, 2036
Sau96I	GGNCC	5 cut(s)	600, 859, 1600, 1601, 2022
SchI	GAGTC	2 cut(s)	423, 1071
ScrFI	CCNGG	5 cut(s)	258, 438, 791, 900, 1151
SduI	GDGCHC	6 cut(s)	709, 974, 1345, 1604, 2137, 2147
SfaNI	GCATC	5 cut(s)	489, 544, 1686, 1701, 1837
SfcI	CTRYAG	3 cut(s)	984, 1076, 1918
SinI	GGWCC	1 cut(s)	859
SmiMI	CAYNNNNRTG	3 cut(s)	269, 595, 1982
SmlI	CTYRAG	3 cut(s)	32, 708, 813
SmoI	CTYRAG	3 cut(s)	32, 708, 813
SphI	GCATGC	1 cut(s)	1657
SseBI	AGGCCT	2 cut(s)	546, 1916
SsiI	CCGC	4 cut(s)	245, 464, 1084, 1681
SspI	AATATT	1 cut(s)	1247
SspMI	CTAG	8 cut(s)	303, 335, 639, 681, 927, 1272, 1430, 1856
SstI	GAGCTC	3 cut(s)	709, 2137, 2147
StuI	AGGCCT	2 cut(s)	546, 1916
StyD4I	CCNGG	5 cut(s)	256, 436, 789, 898, 1149
StyI	CCWWGG	3 cut(s)	603, 629, 670
TaaI	ACNGT	2 cut(s)	28, 290
TaiI	ACGT	2 cut(s)	595, 883
TaqI	TCGA	4 cut(s)	379, 913, 1330, 1694
TauI	GCSGC	1 cut(s)	1086
TfiI	GAWTC	3 cut(s)	477, 994, 2027
Tru1I	TTAA	5 cut(s)	519, 693, 1140, 1214, 1494
Tru9I	TTAA	5 cut(s)	519, 693, 1140, 1214, 1494
TscAI	CASTG	4 cut(s)	592, 1017, 1265, 1486
TseFI	GTSAC	1 cut(s)	934
TseI	GCWGC	3 cut(s)	320, 1952, 2123
Tsp45I	GTSAC	1 cut(s)	934
TspDTI	ATGAA	2 cut(s)	635, 1173
TspGWI	ACGGA	1 cut(s)	730
TspRI	CASTG	4 cut(s)	592, 1017, 1265, 1486
Tth111I	GACNNNGTC	1 cut(s)	1181
VpaK11BI	GGWCC	1 cut(s)	859
XapI	RAATTY	7 cut(s)	188, 695, 818, 1224, 1583, 1669, 2087
XceI	RCATGY	2 cut(s)	1367, 1657
XmnI	GAANNNNTTC	2 cut(s)	1386, 1828
XspI	CTAG	8 cut(s)	303, 335, 639, 681, 927, 1272, 1430, 1856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.