Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008978

Physical Location & Seq

Genomic Coordinates Forward (+)

13861791 .. 13867503

Gene Structure 5713 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1166400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGGGCAGCAGCGGAAAGGGCCGGACAGTTCTGGAGGTGGGAGCCGACGGCGTCGCTCTCATCACCATCATCAACCCACCTGTCAATTCCCTCTCGGTCGATGTGTTGTACAGCTTGAAAGACAGTTATGAGGAGGCCCTGAGAAGAAACGATGTGAAGGCAATTGTTATAACAGGTGCTAAAGGAAAATTTTCGGGTGGTTTCGATATTTCTGCTTTCGGTGGACACCAAGAGGAGCAGAAGCCCGGTTTTATATCCGTGGAGGTAATCACTGATATTTTTGAAGGTGCAAGGAAACCTTCAGTTGCCGCCATTGATGGCCTTGCACTGGGGGGAGGTTTGGAGGTGGCAATGGCATGTCATGCCCGAATATCGACTCCTACTGCACAATTGGGGTTGCCCGAGCTGCAGCTTGGACTCATTCCCGGTTTTGGAGGAACGCAACGGCTTCCACGTCTTGTTGGAATCTCAAGGGCACTTGAGATGATGCTGACATCAAAACCAGTTAAAGGGGAAGAGGCTCATGATTTAGGCCTTGTAGATGCTATTGTTTCACCCGATCAGTTGGTAAGCACTGCACGTAAGTGGGTGTTGGATATCTTGGAGCGTAAAAGACCTTGGGTTGCTAGTCTTCACAAAACCGACAAGTTAGAGCCCCTTGGAGAAGCTAGGGAAATACTTAAATTTGCAAGAGCTCAAGTCCGGAAGCAGGCTCCCAATCTCAAACACCCATTGGTTTGCATTGATGTTGTTGAGGAGGGTATAGCTTCTGGGGGTCGGTCTGGACTTTGGAAGGAGGCTGAATCATTTCAAGGACTTCTGCAGTCAGACACTTGCAAGAGCTTGGTCCATGTCTTCTTTGCTCAACGTGGAACTTCAAAGGTACCTGGGGTTACTGATCGTGGTTTGATGCCTAGACGAGTGACTAAGGTTGCTGTCATTGGTGGAGGGTTAATGGGGTCTGGAATAGCTACCGCATTGATTCTTAGTGGTTATCCAGTGATCCTGAAAGAAGTAAACGATAAGTTCTTGCAGGCTGGGATTGGTAGAGTCAGAGCCAATCTGCAGAGCCGCGTCAAGAAAGGGAAAATGACTCAAGAAAAGTTTGAGAAGACCCTCTCTCTTCTTAAGGGTGTCCTGGATTATGAAAACTTTAGAGATGTAGATATGGTCATAGAGGCCGTTATTGAGAAGGTTTCTCTAAAGCAGCAGATTTTTGCTGATCTTGAAAAATATTGCCCACAACACTGCATACTTGCAAGTAATACTTCCACAATCGACTTGAACCTCATTGGAGAGAAGACCAAGTCTCATGATCGGATTATTGGTGCCCATTTCTTTAGCCCGGCACATGTCATGCCACTTCTGGAAATTGTTCGTACTAATCAGACATCTCCCCAAGCTATTGTTGATTTGCTAGAAGTTGGGAAGAGAATAAAGAAAACACCAGTTGTTGTTGGGAATTGCACAGGATTTGCTGTGAACAGAGTGTTCTTTCCTTATACGCAAGCTGCTCTCTTGCTTGTTGAACGAGGTGCTGATGTTTATCAGATTGATAGGGTAATTGCCAAATTTGGAATGCCTATGGGCCCATTCAGATTGGTTGATCTGGTTGGGTTTGGCGTGGCAATTGCAACCGGCACGCAATTTGTTGTGAACTTTCCTGAGCGGACGTATAAATCAATGCTCATCCCTTTGATGCAGGAGGATGGGAGGGCAGGTGAAACTACTCGAAAAGGGTTCTATACTTATGATGATAGGCGCAAAGCTACCCCAGATACTCAATTGAAAGGATATATTGAAAAGTCAAGAAGCATTTCGGGTGTTTCCATAGATCCTAAGCTACTCAAGTTATCAGAACAGGACATAGTGGAGATGATATTTTTCCCTGTTGTAAATGAGGCATGCCGGGTGCTTGCTGAAGGCATTGCAGTAAAAGCTGCCGACCTTGACATTGCTGCAATCATGGGAATGGGTTTCCCACCATACAGGGGAGGTCTCTTGTTCTGGGCTGATTTACTTGGATCCAAGTACATATATTCAAGATTGGAGGAATGGTCAAATACTTATGGTGAATTTTTCAAGCCTTGCGCCTATTTGGCTGAAAGAGCTGCAAACGGGGCTCCTCTGAGTTCTCAGTCGAACCAAGCAAAATCACGGTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004165 GO:0004300 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009062 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

722

Amino Acids

78.59

Weight (kDa)

9.01

Isoelectric Point (pI)

30.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	16 - 213	1.4e-41	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	28 - 191	4.9e-24	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	310 - 488	3.6e-62	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	491 - 584	7e-21	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008978)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06860
fragaria_vesca	FvH4_4g18500 FvH4_4g18500 FvH4_4g18500
malus_domestica	MD13G1166700.v1.1 MD16G1166400.v1.1
prunus_persica	Prupe.1G133000_v2.0.a1
pyrus_communis	pycom13g14220 pycom16g14060
rosa_chinensis	RchiOBHm_Chr4g0423051
rosa_laevigata	RLG00000007590
rosa_multiflora	Rmu_co8121856.1_g000001 Rmu_sc0006639.1_g000001 Rmu_sc0023965.1_g000001 Rmu_sc0028464.1_g000006
rosa_roxburghii	Rroxscaffold_5G00365130
rosa_rugosa	Rorug04G0183300.1
rosa_samantha	Rh4AG243100 Rh4BG247800 Rh4CG257600 Rh4DG242800
rosa_wichuraiana	Rw4G020970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	170, 2164
AarI	CACCTGC	1 cut(s)	1709
Acc36I	ACCTGC	1 cut(s)	1709
Acc65I	GGTACC	1 cut(s)	883
AccB1I	GGYRCC	2 cut(s)	883, 1328
AccBSI	CCGCTC	1 cut(s)	1669
AccII	CGCG	1 cut(s)	1074
AccIII	TCCGGA	1 cut(s)	702
AciI	CCGC	5 cut(s)	12, 309, 975, 1072, 1669
AclWI	GGATC	4 cut(s)	997, 1829, 2019, 2032
AcsI	RAATTY	4 cut(s)	188, 683, 1571, 2075
AcuI	CTGAAG	2 cut(s)	285, 1941
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	4 cut(s)	110, 885, 1381, 2033
AfiI	CCNNNNNNNGG	5 cut(s)	328, 431, 509, 709, 1991
AflII	CTTAAG	1 cut(s)	1127
AflIII	ACRYGT	1 cut(s)	1351
AjiI	CACGTC	1 cut(s)	455
AjnI	CCWGG	2 cut(s)	886, 1137
AleI	CACNNNNGTG	1 cut(s)	583
Alw21I	GWGCWC	1 cut(s)	697
Alw26I	GTCTC	2 cut(s)	1314, 2003
AlwI	GGATC	4 cut(s)	997, 1829, 2019, 2032
Ama87I	CYCGRG	1 cut(s)	401
Aor13HI	TCCGGA	1 cut(s)	702
AoxI	GGCC	6 cut(s)	19, 135, 319, 532, 1179, 1588
ApaI	GGGCCC	1 cut(s)	1592
ApeKI	GCWGC	9 cut(s)	6, 9, 406, 409, 1207, 1511, 1940, 1958, 2111
ApoI	RAATTY	4 cut(s)	188, 683, 1571, 2075
Asp700I	GAANNNNTTC	4 cut(s)	190, 807, 1374, 1816
Asp718I	GGTACC	1 cut(s)	883
AspLEI	GCGC	2 cut(s)	1764, 2093
AspS9I	GGNCC	5 cut(s)	19, 136, 847, 1588, 1589
AsuC2I	CCSGG	4 cut(s)	246, 426, 1346, 1910
AsuHPI	GGTGA	4 cut(s)	55, 546, 1733, 2084
AvaI	CYCGRG	1 cut(s)	401
AvaII	GGWCC	1 cut(s)	847
BaeGI	GKGCMC	3 cut(s)	478, 1333, 1592
BamHI	GGATCC	1 cut(s)	2024
BanI	GGYRCC	2 cut(s)	883, 1328
BanII	GRGCYC	4 cut(s)	657, 697, 1592, 2125
BbsI	GAAGAC	4 cut(s)	623, 847, 1118, 1307
Bbv12I	GWGCWC	1 cut(s)	697
BbvI	GCAGC	9 cut(s)	18, 21, 393, 421, 1219, 1498, 1927, 1945, 2098
BccI	CCATC	3 cut(s)	74, 311, 1703
BceAI	ACGGC	3 cut(s)	64, 461, 1166
BcgI	CGANNNNNNTGC	2 cut(s)	194, 228
BciT130I	CCWGG	2 cut(s)	888, 1139
BcnI	CCSGG	4 cut(s)	246, 426, 1346, 1910
BcoDI	GTCTC	2 cut(s)	1314, 2003
BfaI	CTAG	4 cut(s)	627, 669, 915, 1418
BfmI	CTRYAG	3 cut(s)	407, 821, 1064
BfrI	CTTAAG	1 cut(s)	1127
BfuAI	ACCTGC	1 cut(s)	1709
BglI	GCCNNNNNGGC	1 cut(s)	2099
Bme1390I	CCNGG	6 cut(s)	246, 426, 888, 1139, 1346, 1910
Bme18I	GGWCC	1 cut(s)	847
BmeT110I	CYCGRG	1 cut(s)	401
BmgBI	CACGTC	1 cut(s)	455
BmgT120I	GGNCC	5 cut(s)	19, 136, 847, 1588, 1589
BmiI	GGNNCC	7 cut(s)	43, 714, 885, 1330, 1590, 2026, 2124
BmrFI	CCNGG	6 cut(s)	246, 426, 888, 1139, 1346, 1910
BmrI	ACTGGG	1 cut(s)	338
BmsI	GCATC	4 cut(s)	477, 532, 900, 1689
BmuI	ACTGGG	1 cut(s)	338
BpiI	GAAGAC	4 cut(s)	623, 847, 1118, 1307
BpmI	CTGGAG	1 cut(s)	53
Bpu10I	CCTNAGC	2 cut(s)	1665, 1839
BpuEI	CTTGAG	5 cut(s)	454, 500, 681, 1080, 1832
BpuMI	CCSGG	4 cut(s)	246, 426, 1346, 1910
BsaAI	YACGTR	1 cut(s)	581
BsaBI	GATNNNNATC	1 cut(s)	1545
BsaHI	GRCGYC	1 cut(s)	51
BsaI	GGTCTC	1 cut(s)	2003
BsaJI	CCNNGG	4 cut(s)	258, 617, 658, 887
BsaWI	WCCGGW	1 cut(s)	702
BsaXI	ACNNNNNCTCC	4 cut(s)	1705, 1735, 2042, 2072
Bsc4I	CCNNNNNNNGG	5 cut(s)	328, 431, 509, 709, 1991
Bse118I	RCCGGY	1 cut(s)	1637
Bse1I	ACTGG	4 cut(s)	333, 503, 998, 1448
Bse3DI	GCAATG	3 cut(s)	357, 1926, 1953
Bse8I	GATNNNNATC	1 cut(s)	1545
BseAI	TCCGGA	1 cut(s)	702
BseBI	CCWGG	2 cut(s)	888, 1139
BseDI	CCNNGG	4 cut(s)	258, 617, 658, 887
BseGI	GGATG	2 cut(s)	1689, 1714
BseJI	GATNNNNATC	1 cut(s)	1545
BseLI	CCNNNNNNNGG	5 cut(s)	328, 431, 509, 709, 1991
BseMI	GCAATG	3 cut(s)	357, 1926, 1953
BseMII	CTCAG	4 cut(s)	131, 1656, 2120, 2150
BseNI	ACTGG	4 cut(s)	333, 503, 998, 1448
BseRI	GAGGAG	4 cut(s)	146, 248, 770, 2115
BseSI	GKGCMC	3 cut(s)	478, 1333, 1592
BseXI	GCAGC	9 cut(s)	18, 21, 393, 421, 1219, 1498, 1927, 1945, 2098
BseYI	CCCAGC	1 cut(s)	1037
BsgI	GTGCAG	2 cut(s)	369, 561
Bsh1236I	CGCG	1 cut(s)	1074
Bsh1285I	CGRYCG	1 cut(s)	99
BshFI	GGCC	6 cut(s)	21, 137, 321, 534, 1181, 1590
BshNI	GGYRCC	2 cut(s)	883, 1328
BsiEI	CGRYCG	1 cut(s)	99
BsiHKAI	GWGCWC	1 cut(s)	697
BsiHKCI	CYCGRG	1 cut(s)	401
BsiSI	CCGG	7 cut(s)	22, 246, 426, 703, 1346, 1638, 1909
BslI	CCNNNNNNNGG	5 cut(s)	328, 431, 509, 709, 1991
BsmAI	GTCTC	2 cut(s)	1314, 2003
BsmI	GAATGC	1 cut(s)	1584
BsnI	GGCC	6 cut(s)	21, 137, 321, 534, 1181, 1590
Bso31I	GGTCTC	1 cut(s)	2003
BsoBI	CYCGRG	1 cut(s)	401
Bsp120I	GGGCCC	1 cut(s)	1588
Bsp1286I	GDGCHC	6 cut(s)	478, 657, 697, 1333, 1592, 2125
Bsp13I	TCCGGA	1 cut(s)	702
Bsp1407I	TGTACA	1 cut(s)	108
Bsp143I	GATC	8 cut(s)	559, 898, 1002, 1222, 1315, 1606, 1834, 2024
BspACI	CCGC	5 cut(s)	12, 309, 975, 1072, 1669
BspANI	GGCC	6 cut(s)	21, 137, 321, 534, 1181, 1590
BspCNI	CTCAG	4 cut(s)	132, 1657, 2121, 2149
BspEI	TCCGGA	1 cut(s)	702
BspFNI	CGCG	1 cut(s)	1074
BspHI	TCATGA	2 cut(s)	523, 1312
BspLI	GGNNCC	7 cut(s)	43, 714, 885, 1330, 1590, 2026, 2124
BspMAI	CTGCAG	3 cut(s)	411, 825, 1068
BspMI	ACCTGC	1 cut(s)	1709
BspPI	GGATC	4 cut(s)	997, 1829, 2019, 2032
BspT107I	GGYRCC	2 cut(s)	883, 1328
BspTI	CTTAAG	1 cut(s)	1127
BspTNI	GGTCTC	1 cut(s)	2003
BsrBI	CCGCTC	1 cut(s)	1669
BsrDI	GCAATG	3 cut(s)	357, 1926, 1953
BsrFI	RCCGGY	1 cut(s)	1637
BsrGI	TGTACA	1 cut(s)	108
BsrI	ACTGG	4 cut(s)	333, 503, 998, 1448
BssAI	RCCGGY	1 cut(s)	1637
BssECI	CCNNGG	4 cut(s)	258, 617, 658, 887
BssMI	GATC	8 cut(s)	559, 898, 1002, 1222, 1315, 1606, 1834, 2024
BssNI	GRCGYC	1 cut(s)	51
BssT1I	CCWWGG	2 cut(s)	617, 658
Bst2UI	CCWGG	2 cut(s)	888, 1139
Bst4CI	ACNGT	3 cut(s)	28, 125, 2160
Bst6I	CTCTTC	3 cut(s)	510, 1128, 1424
BstACI	GRCGYC	1 cut(s)	51
BstAFI	CTTAAG	1 cut(s)	1127
BstAPI	GCANNNNNTGC	1 cut(s)	362
BstAUI	TGTACA	1 cut(s)	108
BstBAI	YACGTR	1 cut(s)	581
BstC8I	GCNNGC	6 cut(s)	711, 1035, 1509, 1643, 1906, 1917
BstDEI	CTNAG	7 cut(s)	140, 927, 986, 1665, 1839, 2129, 2136
BstDSI	CCRYGG	1 cut(s)	258
BstF5I	GGATG	2 cut(s)	1689, 1714
BstFNI	CGCG	1 cut(s)	1074
BstHHI	GCGC	2 cut(s)	1764, 2093
BstKTI	GATC	8 cut(s)	562, 901, 1005, 1225, 1318, 1609, 1837, 2027
BstMAI	GTCTC	2 cut(s)	1314, 2003
BstMBI	GATC	8 cut(s)	559, 898, 1002, 1222, 1315, 1606, 1834, 2024
BstMCI	CGRYCG	1 cut(s)	99
BstMWI	GCNNNNNNNGC	7 cut(s)	18, 362, 406, 1937, 2099, 2108, 2120
BstNI	CCWGG	2 cut(s)	888, 1139
BstNSI	RCATGY	3 cut(s)	360, 1355, 1908
BstSCI	CCNGG	6 cut(s)	244, 424, 886, 1137, 1344, 1908
BstSFI	CTRYAG	3 cut(s)	407, 821, 1064
BstSLI	GKGCMC	3 cut(s)	478, 1333, 1592
BstUI	CGCG	1 cut(s)	1074
BstV1I	GCAGC	9 cut(s)	18, 21, 393, 421, 1219, 1498, 1927, 1945, 2098
BstV2I	GAAGAC	4 cut(s)	623, 847, 1118, 1307
BstX2I	RGATCY	2 cut(s)	1834, 2024
BstYI	RGATCY	2 cut(s)	1834, 2024
BsuRI	GGCC	6 cut(s)	21, 137, 321, 534, 1181, 1590
BtgI	CCRYGG	1 cut(s)	258
BtrI	CACGTC	1 cut(s)	455
BtsCI	GGATG	2 cut(s)	1689, 1714
BtsI	GCAGTG	2 cut(s)	573, 1246
BtsIMutI	CAGTG	5 cut(s)	270, 326, 573, 1005, 1246
BveI	ACCTGC	1 cut(s)	1709
Cac8I	GCNNGC	6 cut(s)	711, 1035, 1509, 1643, 1906, 1917
CciI	TCATGA	2 cut(s)	523, 1312
CfoI	GCGC	2 cut(s)	1764, 2093
Cfr10I	RCCGGY	1 cut(s)	1637
Cfr13I	GGNCC	5 cut(s)	19, 136, 847, 1588, 1589
CseI	GACGC	2 cut(s)	40, 1063
Csp6I	GTAC	4 cut(s)	109, 884, 1380, 2032
CviAII	CATG	9 cut(s)	357, 362, 524, 851, 1313, 1352, 1357, 1905, 1966
CviQI	GTAC	4 cut(s)	109, 884, 1380, 2032
DdeI	CTNAG	7 cut(s)	140, 927, 986, 1665, 1839, 2129, 2136
DpnI	GATC	8 cut(s)	561, 900, 1004, 1224, 1317, 1608, 1836, 2026
DpnII	GATC	8 cut(s)	559, 898, 1002, 1222, 1315, 1606, 1834, 2024
Eam1104I	CTCTTC	3 cut(s)	510, 1128, 1424
EarI	CTCTTC	3 cut(s)	510, 1128, 1424
Ecl136II	GAGCTC	1 cut(s)	695
Eco130I	CCWWGG	2 cut(s)	617, 658
Eco147I	AGGCCT	1 cut(s)	534
Eco24I	GRGCYC	4 cut(s)	657, 697, 1592, 2125
Eco31I	GGTCTC	1 cut(s)	2003
Eco32I	GATATC	1 cut(s)	598
Eco47I	GGWCC	1 cut(s)	847
Eco53kI	GAGCTC	1 cut(s)	695
Eco57I	CTGAAG	2 cut(s)	285, 1941
Eco88I	CYCGRG	1 cut(s)	401
EcoICRI	GAGCTC	1 cut(s)	695
EcoO109I	RGGNCCY	1 cut(s)	136
EcoRII	CCWGG	2 cut(s)	886, 1137
EcoRV	GATATC	1 cut(s)	598
EcoT14I	CCWWGG	2 cut(s)	617, 658
EcoT38I	GRGCYC	4 cut(s)	657, 697, 1592, 2125
ErhI	CCWWGG	2 cut(s)	617, 658
FaeI	CATG	9 cut(s)	360, 365, 527, 854, 1316, 1355, 1360, 1908, 1969
FalI	AAGNNNNNCTT	4 cut(s)	283, 315, 1252, 1284
FatI	CATG	9 cut(s)	356, 361, 523, 850, 1312, 1351, 1356, 1904, 1965
FokI	GGATG	2 cut(s)	1676, 1721
FriOI	GRGCYC	4 cut(s)	657, 697, 1592, 2125
FspBI	CTAG	4 cut(s)	627, 669, 915, 1418
GlaI	GCGC	2 cut(s)	1763, 2092
GsaI	CCCAGC	1 cut(s)	1041
GsuI	CTGGAG	1 cut(s)	53
HaeIII	GGCC	6 cut(s)	21, 137, 321, 534, 1181, 1590
HapII	CCGG	7 cut(s)	22, 246, 426, 703, 1346, 1638, 1909
HgaI	GACGC	2 cut(s)	40, 1063
HhaI	GCGC	2 cut(s)	1764, 2093
Hin1I	GRCGYC	1 cut(s)	51
Hin1II	CATG	9 cut(s)	360, 365, 527, 854, 1316, 1355, 1360, 1908, 1969
Hin6I	GCGC	2 cut(s)	1762, 2091
HinP1I	GCGC	2 cut(s)	1762, 2091
HinfI	GANTC	7 cut(s)	376, 417, 465, 803, 982, 1050, 1093
HpaII	CCGG	7 cut(s)	22, 246, 426, 703, 1346, 1638, 1909
HphI	GGTGA	4 cut(s)	55, 546, 1733, 2084
Hpy166II	GTNNAC	4 cut(s)	224, 1018, 1483, 1657
Hpy188I	TCNGA	8 cut(s)	829, 1055, 1320, 1389, 1551, 1598, 1858, 2130
Hpy8I	GTNNAC	4 cut(s)	224, 1018, 1483, 1657
Hpy99I	CGWCG	2 cut(s)	50, 56
HpyAV	CCTTC	6 cut(s)	151, 278, 309, 787, 1186, 1916
HpyCH4III	ACNGT	3 cut(s)	28, 125, 2160
HpyCH4IV	ACGT	4 cut(s)	454, 580, 868, 1673
HpyF10VI	GCNNNNNNNGC	7 cut(s)	18, 362, 406, 1937, 2099, 2108, 2120
HpyF3I	CTNAG	7 cut(s)	140, 927, 986, 1665, 1839, 2129, 2136
HpySE526I	ACGT	4 cut(s)	454, 580, 868, 1673
Hsp92I	GRCGYC	1 cut(s)	51
Hsp92II	CATG	9 cut(s)	360, 365, 527, 854, 1316, 1355, 1360, 1908, 1969
HspAI	GCGC	2 cut(s)	1762, 2091
Kpn2I	TCCGGA	1 cut(s)	702
KpnI	GGTACC	1 cut(s)	887
Kzo9I	GATC	8 cut(s)	559, 898, 1002, 1222, 1315, 1606, 1834, 2024
LmnI	GCTCC	5 cut(s)	41, 235, 604, 718, 2128
Lsp1109I	GCAGC	9 cut(s)	18, 21, 393, 421, 1219, 1498, 1927, 1945, 2098
LweI	GCATC	4 cut(s)	477, 532, 900, 1689
MaeI	CTAG	4 cut(s)	627, 669, 915, 1418
MaeII	ACGT	4 cut(s)	454, 580, 868, 1673
MaeIII	GTNAC	2 cut(s)	892, 922
MalI	GATC	8 cut(s)	561, 900, 1004, 1224, 1317, 1608, 1836, 2026
MbiI	CCGCTC	1 cut(s)	1669
MboI	GATC	8 cut(s)	559, 898, 1002, 1222, 1315, 1606, 1834, 2024
MboII	GAAGA	8 cut(s)	156, 527, 623, 847, 1115, 1123, 1312, 1441
MfeI	CAATTG	4 cut(s)	162, 389, 1629, 1784
MflI	RGATCY	2 cut(s)	1834, 2024
MhlI	GDGCHC	6 cut(s)	478, 657, 697, 1333, 1592, 2125
MlyI	GAGTC	4 cut(s)	370, 411, 1059, 1087
MmeI	TCCRAC	2 cut(s)	442, 573
MroI	TCCGGA	1 cut(s)	702
MroXI	GAANNNNTTC	4 cut(s)	190, 807, 1374, 1816
MseI	TTAA	4 cut(s)	507, 681, 953, 1128
MslI	CAYNNNNRTG	2 cut(s)	583, 1970
MspA1I	CMGCKG	1 cut(s)	12
MspCI	CTTAAG	1 cut(s)	1127
MspI	CCGG	7 cut(s)	22, 246, 426, 703, 1346, 1638, 1909
MspR9I	CCNGG	6 cut(s)	246, 426, 888, 1139, 1346, 1910
MunI	CAATTG	4 cut(s)	162, 389, 1629, 1784
Mva1269I	GAATGC	1 cut(s)	1584
MvaI	CCWGG	2 cut(s)	888, 1139
MvnI	CGCG	1 cut(s)	1074
MwoI	GCNNNNNNNGC	7 cut(s)	18, 362, 406, 1937, 2099, 2108, 2120
NciI	CCSGG	4 cut(s)	246, 426, 1346, 1910
NdeII	GATC	8 cut(s)	559, 898, 1002, 1222, 1315, 1606, 1834, 2024
NlaIII	CATG	9 cut(s)	360, 365, 527, 854, 1316, 1355, 1360, 1908, 1969
NlaIV	GGNNCC	7 cut(s)	43, 714, 885, 1330, 1590, 2026, 2124
NmuCI	GTSAC	1 cut(s)	922
NspI	RCATGY	3 cut(s)	360, 1355, 1908
OliI	CACNNNNGTG	1 cut(s)	583
PaeI	GCATGC	1 cut(s)	1908
PagI	TCATGA	2 cut(s)	523, 1312
PaqCI	CACCTGC	1 cut(s)	1709
PceI	AGGCCT	1 cut(s)	534
PciI	ACATGT	1 cut(s)	1351
PcsI	WCGNNNNNNNCGW	1 cut(s)	451
PctI	GAATGC	1 cut(s)	1584
PdmI	GAANNNNTTC	4 cut(s)	190, 807, 1374, 1816
PfeI	GAWTC	3 cut(s)	465, 803, 982
PflFI	GACNNNGTC	2 cut(s)	50, 1306
PfoI	TCCNGGA	1 cut(s)	1137
PleI	GAGTC	4 cut(s)	370, 411, 1058, 1087
PpsI	GAGTC	4 cut(s)	370, 411, 1058, 1087
Ppu21I	YACGTR	1 cut(s)	581
PscI	ACATGT	1 cut(s)	1351
PsiI	TTATAA	2 cut(s)	170, 2164
Psp124BI	GAGCTC	1 cut(s)	697
Psp6I	CCWGG	2 cut(s)	886, 1137
PspFI	CCCAGC	1 cut(s)	1037
PspGI	CCWGG	2 cut(s)	886, 1137
PspN4I	GGNNCC	7 cut(s)	43, 714, 885, 1330, 1590, 2026, 2124
PspOMI	GGGCCC	1 cut(s)	1588
PspPI	GGNCC	5 cut(s)	19, 136, 847, 1588, 1589
PstI	CTGCAG	3 cut(s)	411, 825, 1068
PsuI	RGATCY	2 cut(s)	1834, 2024
PsyI	GACNNNGTC	2 cut(s)	50, 1306
RsaI	GTAC	4 cut(s)	110, 885, 1381, 2033
RsaNI	GTAC	4 cut(s)	109, 884, 1380, 2032
RseI	CAYNNNNRTG	2 cut(s)	583, 1970
SacI	GAGCTC	1 cut(s)	697
SaqAI	TTAA	4 cut(s)	507, 681, 953, 1128
Sau3AI	GATC	8 cut(s)	559, 898, 1002, 1222, 1315, 1606, 1834, 2024
Sau96I	GGNCC	5 cut(s)	19, 136, 847, 1588, 1589
SchI	GAGTC	4 cut(s)	370, 411, 1059, 1087
ScrFI	CCNGG	6 cut(s)	246, 426, 888, 1139, 1346, 1910
SduI	GDGCHC	6 cut(s)	478, 657, 697, 1333, 1592, 2125
SfaNI	GCATC	4 cut(s)	477, 532, 900, 1689
SfcI	CTRYAG	3 cut(s)	407, 821, 1064
SinI	GGWCC	1 cut(s)	847
SmiMI	CAYNNNNRTG	2 cut(s)	583, 1970
SmlI	CTYRAG	6 cut(s)	469, 479, 696, 1095, 1127, 1847
SmoI	CTYRAG	6 cut(s)	469, 479, 696, 1095, 1127, 1847
SphI	GCATGC	1 cut(s)	1908
SseBI	AGGCCT	1 cut(s)	534
SsiI	CCGC	5 cut(s)	12, 309, 975, 1072, 1669
SspI	AATATT	1 cut(s)	1235
SspMI	CTAG	4 cut(s)	627, 669, 915, 1418
SstI	GAGCTC	1 cut(s)	697
StuI	AGGCCT	1 cut(s)	534
StyD4I	CCNGG	6 cut(s)	244, 424, 886, 1137, 1344, 1908
StyI	CCWWGG	2 cut(s)	617, 658
TaaI	ACNGT	3 cut(s)	28, 125, 2160
TaiI	ACGT	4 cut(s)	457, 583, 871, 1676
TaqI	TCGA	6 cut(s)	99, 204, 374, 1278, 1732, 2141
TaqII	GACCGA	2 cut(s)	85, 768
TatI	WGTACW	2 cut(s)	108, 2031
TauI	GCSGC	2 cut(s)	311, 1074
TfiI	GAWTC	3 cut(s)	465, 803, 982
Tru1I	TTAA	4 cut(s)	507, 681, 953, 1128
Tru9I	TTAA	4 cut(s)	507, 681, 953, 1128
TscAI	CASTG	5 cut(s)	277, 333, 580, 1005, 1253
TseFI	GTSAC	1 cut(s)	922
TseI	GCWGC	9 cut(s)	6, 9, 406, 409, 1207, 1511, 1940, 1958, 2111
Tsp45I	GTSAC	1 cut(s)	922
TspDTI	ATGAA	1 cut(s)	1161
TspGWI	ACGGA	1 cut(s)	247
TspRI	CASTG	5 cut(s)	277, 333, 580, 1005, 1253
Tth111I	GACNNNGTC	2 cut(s)	50, 1306
Vha464I	CTTAAG	1 cut(s)	1127
VpaK11BI	GGWCC	1 cut(s)	847
XapI	RAATTY	4 cut(s)	188, 683, 1571, 2075
XceI	RCATGY	3 cut(s)	360, 1355, 1908
XmnI	GAANNNNTTC	4 cut(s)	190, 807, 1374, 1816
XspI	CTAG	4 cut(s)	627, 669, 915, 1418

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.