Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008978

Physical Location & Seq

Genomic Coordinates Forward (+)

13335536 .. 13341436

Gene Structure 5901 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1166700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGGGCAGCAGCGGAAAGGGCCGGACAGTTCTGGAAGTGGGAGCCGATGGCGTGGCTCTCATCACCATCATCAACCCACCTGTCAATTCCCTCTCAATCGATGTGTTATTCAGCTTGAAAGACAGTTATGAGGAGGCCTTGCGAAGAAATGATGTGAAGGCGATTGTTGTAACAGGTGCTAAAGGAAAATTTTCGGGGGGTTTCGATATTACTGCTTTTGGTGGACTCCAAGAGGGGCAGAAGCAGGAGCCGAAGCCCGGTTTTATATCCGTGGAGGTTATCACTGACATTGTTGAAGGTGCTAGGAAACCGTCAGTTGCCGCCATTGATGGCCTTGCACTGGGGGGAGGTTTGGAGGTGGCAATGGCATGCCATGCCCGAATATCGACTCCTACTGCACAATTAGGGTTGCCCGAGCTGCAGCTTGGGCTCATTCCTGGATTTGGAGGAACACAACGGCTTCCACGTCTTGTTGGAATCTCAAAGGCACTTGAGATGATGCTGACATCAAAACCAGTTAAAGGGGAAGAGGCTCATGATTTAGGCCTTGTCGATGCTATTGTTTCACCTGATCAGTTGGTAAGCACTGCACGTAGGTGGGCGTTGGATATCCTGGAGCGTAGAAGACCGTGGGTTGCTAGTCTTCACAAAACTGACAAGTTAGAGCCCCTTGGAGAAGCTAGGGAAATACTTAAATTTGCAAGAGCTCAAGTCCGGAAGCAGGCTCCCAATCTCAAACACCCATTGGTTTGTATTGATGTTGTTGAGGAGGGTATAGCTTCTGGTGGTCGGTCTGGACTTTGGAAGGAGGCTGAATCATTTCAAGGACTTCTGCGGTCTGACACTTGCAAGAGCTTAGTCCACGTCTTCTTTGCTCAACGTGGAACATCAAAGGTACCTGGGGTTACTGATCGTGGTTTGATGCCTAGACGAGTGACTAAAGTTGCTGTCATTGGTGGAGGGTTAATGGGATCTGGAATAGCTACTGCATTGATTCTTAGTGGTTATCCTGTGATCCTGAAAGAAGTAAATGATAAGTTCTTGCAGGCTGGGATTAGTAGAGTCAGAGCCAATCTGCAGAGCCGCGTCAAGAAGGGGAAAATGACCCAAGAAAAGTTTGAGAAGACGACCTCTATTCTTAAGGGTGTCCTGGATTATGATAGCTTTAGAGATGTGGATATGGTCATAGAGGCCGTTATTGAGAAGGTTTCTCTAAAGCAGCAGATTTTTGCTGATCTTGAAAAATATTGCCCACAACACTGCATACTCGCAAGTAATACTTCCACAATCGACTTGAACCTCATTGGAGAGAAAACCAAGTGTCATGATCGGATTATTGGTGCCCATTTCTTTAGTCCGGCACATGTCATGCCGCTTCTGGAAATTGTTCGTACTAATCAGACATCTCCCCAAGTTATTGTTGATTTGCTAGAAGTTGGGAAAAGAATAAAGAAAACCCCAGTGGTTGTTGGGAATTGCACAGGATTTGCTGTGAACAGAATGTTCTTTCCTTATACGCAGGCTGCTCTCTTGCTTGTTGAACATGGTGCCGATGTTTATCAGATTGATAGGGTAATCACCAAATTCGGAATGCCTATGGGCCCATTCAGATTGGCTGATCTGGTTGGGTTTGGCGTGGCAATTGCAACTGGCACACAATTTGTTGTGAACTTTCCTGAGCGGACATATAAATCATTGCTAATCCCTTTGATGCAGGAGGATGGGAGGGCAGGTGAAACTACACGAAAGGGGTTCTATACTTATGATGACAGGCGCAAAGCTAGCCCAGATCCTCAGTTGAAAGGATATATTGAGAAGTCAAGGAGCATTTCGGGTGTTTCCATAGATTCTAAGCTGCTGAAGTTATCAGAAAAGGACATTGTTGAGATGATATTTTTCCCTGTCGTAAATGAGGCATGCCGGGTGCTTGCTGAAGGCATTGCTGTGAAAGCTGCCGACCTTGACATTGCTGCAATTATGGGCATGGGTTTCCCACCATACAGGGGAGGTATCTTGTTCTGGGCTGATTTACTTGGATCCGAATATATATATTCACGATTGGAGGAATGGTCAAATACTTATGGTGAATTTTTCAAGCCTTGCGCCTATTTGGCTGAAAGAGCTTCAAACGGGGCTTCTCTGAGTTCTCCGTTGAAGCAATCAAAATCTCGGTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004165 GO:0004300 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009062 GO:0009514 GO:0009987 GO:0016042 GO:0016054 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0030258 GO:0032787 GO:0034440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0055114 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

726

Amino Acids

78.98

Weight (kDa)

9.04

Isoelectric Point (pI)

36.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	16 - 216	1.6e-41	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	28 - 196	3.2e-24	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	314 - 492	4.6e-61	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	495 - 588	3.3e-21	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008978)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06860
fragaria_vesca	FvH4_4g18500 FvH4_4g18500 FvH4_4g18500
malus_domestica	MD13G1166700.v1.1 MD16G1166400.v1.1
prunus_persica	Prupe.1G133000_v2.0.a1
pyrus_communis	pycom13g14220 pycom16g14060
rosa_chinensis	RchiOBHm_Chr4g0423051
rosa_laevigata	RLG00000007590
rosa_multiflora	Rmu_co8121856.1_g000001 Rmu_sc0006639.1_g000001 Rmu_sc0023965.1_g000001 Rmu_sc0028464.1_g000006
rosa_roxburghii	Rroxscaffold_5G00365130
rosa_rugosa	Rorug04G0183300.1
rosa_samantha	Rh4AG243100 Rh4BG247800 Rh4CG257600 Rh4DG242800
rosa_wichuraiana	Rw4G020970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2176
AarI	CACCTGC	1 cut(s)	1721
Acc36I	ACCTGC	1 cut(s)	1721
Acc65I	GGTACC	1 cut(s)	895
AccB1I	GGYRCC	3 cut(s)	895, 1340, 1547
AccBSI	CCGCTC	1 cut(s)	1681
AccII	CGCG	1 cut(s)	1086
AccIII	TCCGGA	1 cut(s)	714
AciI	CCGC	6 cut(s)	12, 321, 835, 1084, 1373, 1681
AclWI	GGATC	5 cut(s)	979, 1009, 1784, 2031, 2044
AcsI	RAATTY	4 cut(s)	188, 695, 1583, 2087
AcuI	CTGAAG	2 cut(s)	1880, 1953
AfaI	GTAC	2 cut(s)	897, 1393
AfiI	CCNNNNNNNGG	6 cut(s)	257, 340, 443, 521, 721, 2003
AflII	CTTAAG	1 cut(s)	1139
AflIII	ACRYGT	1 cut(s)	1363
AjiI	CACGTC	2 cut(s)	467, 865
AjnI	CCWGG	4 cut(s)	436, 612, 898, 1149
AleI	CACNNNNGTG	1 cut(s)	595
Alw21I	GWGCWC	1 cut(s)	709
AlwI	GGATC	5 cut(s)	979, 1009, 1784, 2031, 2044
Ama87I	CYCGRG	1 cut(s)	413
Aor13HI	TCCGGA	1 cut(s)	714
AoxI	GGCC	6 cut(s)	19, 135, 331, 544, 1191, 1600
ApaI	GGGCCC	1 cut(s)	1604
ApeKI	GCWGC	9 cut(s)	6, 9, 418, 421, 1219, 1523, 1855, 1952, 1970
ApoI	RAATTY	4 cut(s)	188, 695, 1583, 2087
Asp700I	GAANNNNTTC	3 cut(s)	190, 819, 1386
Asp718I	GGTACC	1 cut(s)	895
AspLEI	GCGC	2 cut(s)	1776, 2105
AspS9I	GGNCC	3 cut(s)	19, 1600, 1601
AsuC2I	CCSGG	2 cut(s)	258, 1922
AsuHPI	GGTGA	5 cut(s)	55, 558, 1570, 1745, 2096
AsuNHI	GCTAGC	1 cut(s)	1781
AvaI	CYCGRG	1 cut(s)	413
BaeGI	GKGCMC	2 cut(s)	1345, 1604
BamHI	GGATCC	1 cut(s)	2036
BanI	GGYRCC	3 cut(s)	895, 1340, 1547
BanII	GRGCYC	4 cut(s)	432, 669, 709, 1604
BbsI	GAAGAC	4 cut(s)	631, 635, 859, 1130
Bbv12I	GWGCWC	1 cut(s)	709
BbvI	GCAGC	9 cut(s)	18, 21, 405, 433, 1231, 1510, 1842, 1939, 1957
BccI	CCATC	4 cut(s)	41, 74, 323, 1715
BceAI	ACGGC	2 cut(s)	473, 1178
BcgI	CGANNNNNNTGC	2 cut(s)	194, 228
BciT130I	CCWGG	4 cut(s)	438, 614, 900, 1151
BclI	TGATCA	1 cut(s)	571
BcnI	CCSGG	2 cut(s)	258, 1922
BfaI	CTAG	6 cut(s)	303, 639, 681, 927, 1430, 1782
BfmI	CTRYAG	2 cut(s)	419, 1076
BfrI	CTTAAG	1 cut(s)	1139
BfuAI	ACCTGC	1 cut(s)	1721
BglI	GCCNNNNNGGC	1 cut(s)	2111
Bme1390I	CCNGG	6 cut(s)	258, 438, 614, 900, 1151, 1922
BmeT110I	CYCGRG	1 cut(s)	413
BmgBI	CACGTC	2 cut(s)	467, 865
BmgT120I	GGNCC	3 cut(s)	19, 1600, 1601
BmiI	GGNNCC	8 cut(s)	43, 249, 726, 897, 1342, 1549, 1602, 2038
BmrFI	CCNGG	6 cut(s)	258, 438, 614, 900, 1151, 1922
BmrI	ACTGGG	2 cut(s)	350, 1454
BmsI	GCATC	4 cut(s)	489, 544, 912, 1701
BmtI	GCTAGC	1 cut(s)	1785
BmuI	ACTGGG	2 cut(s)	350, 1454
BpiI	GAAGAC	4 cut(s)	631, 635, 859, 1130
BpmI	CTGGAG	1 cut(s)	635
Bpu10I	CCTNAGC	1 cut(s)	1677
BpuEI	CTTGAG	2 cut(s)	512, 693
BpuMI	CCSGG	2 cut(s)	258, 1922
Bsa29I	ATCGAT	1 cut(s)	99
BsaAI	YACGTR	1 cut(s)	593
BsaBI	GATNNNNATC	1 cut(s)	1557
BsaJI	CCNNGG	4 cut(s)	270, 629, 670, 899
BsaWI	WCCGGW	1 cut(s)	714
BsaXI	ACNNNNNCTCC	4 cut(s)	1717, 1747, 2054, 2084
Bsc4I	CCNNNNNNNGG	6 cut(s)	257, 340, 443, 521, 721, 2003
Bse1I	ACTGG	4 cut(s)	345, 515, 1460, 1654
Bse3DI	GCAATG	4 cut(s)	369, 1694, 1938, 1965
Bse8I	GATNNNNATC	1 cut(s)	1557
BseAI	TCCGGA	1 cut(s)	714
BseBI	CCWGG	4 cut(s)	438, 614, 900, 1151
BseCI	ATCGAT	1 cut(s)	99
BseDI	CCNNGG	4 cut(s)	270, 629, 670, 899
BseGI	GGATG	1 cut(s)	1726
BseJI	GATNNNNATC	1 cut(s)	1557
BseLI	CCNNNNNNNGG	6 cut(s)	257, 340, 443, 521, 721, 2003
BseMI	GCAATG	4 cut(s)	369, 1694, 1938, 1965
BseMII	CTCAG	3 cut(s)	1668, 1808, 2132
BseNI	ACTGG	4 cut(s)	345, 515, 1460, 1654
BseRI	GAGGAG	2 cut(s)	146, 782
BseSI	GKGCMC	2 cut(s)	1345, 1604
BseXI	GCAGC	9 cut(s)	18, 21, 405, 433, 1231, 1510, 1842, 1939, 1957
BseYI	CCCAGC	1 cut(s)	1049
BsgI	GTGCAG	2 cut(s)	381, 573
Bsh1236I	CGCG	1 cut(s)	1086
BshFI	GGCC	6 cut(s)	21, 137, 333, 546, 1193, 1602
BshNI	GGYRCC	3 cut(s)	895, 1340, 1547
BshVI	ATCGAT	1 cut(s)	99
BsiHKAI	GWGCWC	1 cut(s)	709
BsiHKCI	CYCGRG	1 cut(s)	413
BsiSI	CCGG	5 cut(s)	22, 258, 715, 1358, 1921
BslI	CCNNNNNNNGG	6 cut(s)	257, 340, 443, 521, 721, 2003
BsmI	GAATGC	1 cut(s)	1596
BsnI	GGCC	6 cut(s)	21, 137, 333, 546, 1193, 1602
BsoBI	CYCGRG	1 cut(s)	413
Bsp120I	GGGCCC	1 cut(s)	1600
Bsp1286I	GDGCHC	5 cut(s)	432, 669, 709, 1345, 1604
Bsp13I	TCCGGA	1 cut(s)	714
Bsp143I	GATC	9 cut(s)	571, 910, 971, 1014, 1234, 1327, 1618, 1789, 2036
BspACI	CCGC	6 cut(s)	12, 321, 835, 1084, 1373, 1681
BspANI	GGCC	6 cut(s)	21, 137, 333, 546, 1193, 1602
BspCNI	CTCAG	3 cut(s)	1669, 1807, 2133
BspDI	ATCGAT	1 cut(s)	99
BspEI	TCCGGA	1 cut(s)	714
BspFNI	CGCG	1 cut(s)	1086
BspHI	TCATGA	2 cut(s)	535, 1324
BspLI	GGNNCC	8 cut(s)	43, 249, 726, 897, 1342, 1549, 1602, 2038
BspMAI	CTGCAG	2 cut(s)	423, 1080
BspMI	ACCTGC	1 cut(s)	1721
BspOI	GCTAGC	1 cut(s)	1785
BspPI	GGATC	5 cut(s)	979, 1009, 1784, 2031, 2044
BspT107I	GGYRCC	3 cut(s)	895, 1340, 1547
BspTI	CTTAAG	1 cut(s)	1139
BsrBI	CCGCTC	1 cut(s)	1681
BsrDI	GCAATG	4 cut(s)	369, 1694, 1938, 1965
BsrI	ACTGG	4 cut(s)	345, 515, 1460, 1654
BssECI	CCNNGG	4 cut(s)	270, 629, 670, 899
BssMI	GATC	9 cut(s)	571, 910, 971, 1014, 1234, 1327, 1618, 1789, 2036
BssT1I	CCWWGG	1 cut(s)	670
Bst2UI	CCWGG	4 cut(s)	438, 614, 900, 1151
Bst4CI	ACNGT	4 cut(s)	28, 125, 312, 630
Bst6I	CTCTTC	1 cut(s)	522
BstAFI	CTTAAG	1 cut(s)	1139
BstAPI	GCANNNNNTGC	1 cut(s)	374
BstBAI	YACGTR	1 cut(s)	593
BstC8I	GCNNGC	7 cut(s)	370, 723, 1047, 1521, 1783, 1918, 1929
BstDEI	CTNAG	6 cut(s)	856, 998, 1677, 1794, 1851, 2141
BstDSI	CCRYGG	2 cut(s)	270, 629
BstF5I	GGATG	1 cut(s)	1726
BstFNI	CGCG	1 cut(s)	1086
BstHHI	GCGC	2 cut(s)	1776, 2105
BstKTI	GATC	9 cut(s)	574, 913, 974, 1017, 1237, 1330, 1621, 1792, 2039
BstMBI	GATC	9 cut(s)	571, 910, 971, 1014, 1234, 1327, 1618, 1789, 2036
BstMWI	GCNNNNNNNGC	8 cut(s)	18, 374, 418, 427, 1782, 1949, 2111, 2120
BstNI	CCWGG	4 cut(s)	438, 614, 900, 1151
BstNSI	RCATGY	3 cut(s)	372, 1367, 1920
BstSCI	CCNGG	6 cut(s)	256, 436, 612, 898, 1149, 1920
BstSFI	CTRYAG	2 cut(s)	419, 1076
BstSLI	GKGCMC	2 cut(s)	1345, 1604
BstUI	CGCG	1 cut(s)	1086
BstV1I	GCAGC	9 cut(s)	18, 21, 405, 433, 1231, 1510, 1842, 1939, 1957
BstV2I	GAAGAC	4 cut(s)	631, 635, 859, 1130
BstX2I	RGATCY	3 cut(s)	971, 1789, 2036
BstYI	RGATCY	3 cut(s)	971, 1789, 2036
Bsu15I	ATCGAT	1 cut(s)	99
BsuRI	GGCC	6 cut(s)	21, 137, 333, 546, 1193, 1602
BsuTUI	ATCGAT	1 cut(s)	99
BtgI	CCRYGG	2 cut(s)	270, 629
BtrI	CACGTC	2 cut(s)	467, 865
BtsCI	GGATG	1 cut(s)	1726
BtsI	GCAGTG	2 cut(s)	585, 1258
BtsIMutI	CAGTG	5 cut(s)	282, 338, 585, 1258, 1467
BveI	ACCTGC	1 cut(s)	1721
Cac8I	GCNNGC	7 cut(s)	370, 723, 1047, 1521, 1783, 1918, 1929
CciI	TCATGA	2 cut(s)	535, 1324
CfoI	GCGC	2 cut(s)	1776, 2105
Cfr13I	GGNCC	3 cut(s)	19, 1600, 1601
ClaI	ATCGAT	1 cut(s)	99
CseI	GACGC	1 cut(s)	1075
Csp6I	GTAC	2 cut(s)	896, 1392
CviAII	CATG	9 cut(s)	369, 374, 536, 1325, 1364, 1369, 1544, 1917, 1984
CviQI	GTAC	2 cut(s)	896, 1392
DdeI	CTNAG	6 cut(s)	856, 998, 1677, 1794, 1851, 2141
DpnI	GATC	9 cut(s)	573, 912, 973, 1016, 1236, 1329, 1620, 1791, 2038
DpnII	GATC	9 cut(s)	571, 910, 971, 1014, 1234, 1327, 1618, 1789, 2036
Eam1104I	CTCTTC	1 cut(s)	522
EarI	CTCTTC	1 cut(s)	522
Ecl136II	GAGCTC	1 cut(s)	707
Eco130I	CCWWGG	1 cut(s)	670
Eco147I	AGGCCT	2 cut(s)	137, 546
Eco24I	GRGCYC	4 cut(s)	432, 669, 709, 1604
Eco32I	GATATC	1 cut(s)	610
Eco53kI	GAGCTC	1 cut(s)	707
Eco57I	CTGAAG	2 cut(s)	1880, 1953
Eco88I	CYCGRG	1 cut(s)	413
EcoICRI	GAGCTC	1 cut(s)	707
EcoRII	CCWGG	4 cut(s)	436, 612, 898, 1149
EcoRV	GATATC	1 cut(s)	610
EcoT14I	CCWWGG	1 cut(s)	670
EcoT38I	GRGCYC	4 cut(s)	432, 669, 709, 1604
ErhI	CCWWGG	1 cut(s)	670
FaeI	CATG	9 cut(s)	372, 377, 539, 1328, 1367, 1372, 1547, 1920, 1987
FalI	AAGNNNNNCTT	2 cut(s)	1264, 1296
FatI	CATG	9 cut(s)	368, 373, 535, 1324, 1363, 1368, 1543, 1916, 1983
FbaI	TGATCA	1 cut(s)	571
FokI	GGATG	1 cut(s)	1733
FriOI	GRGCYC	4 cut(s)	432, 669, 709, 1604
FspBI	CTAG	6 cut(s)	303, 639, 681, 927, 1430, 1782
GlaI	GCGC	2 cut(s)	1775, 2104
GsaI	CCCAGC	1 cut(s)	1053
GsuI	CTGGAG	1 cut(s)	635
HaeIII	GGCC	6 cut(s)	21, 137, 333, 546, 1193, 1602
HapII	CCGG	5 cut(s)	22, 258, 715, 1358, 1921
HgaI	GACGC	1 cut(s)	1075
HhaI	GCGC	2 cut(s)	1776, 2105
Hin1II	CATG	9 cut(s)	372, 377, 539, 1328, 1367, 1372, 1547, 1920, 1987
Hin6I	GCGC	2 cut(s)	1774, 2103
HinP1I	GCGC	2 cut(s)	1774, 2103
HinfI	GANTC	7 cut(s)	225, 388, 477, 815, 994, 1062, 1847
HpaII	CCGG	5 cut(s)	22, 258, 715, 1358, 1921
HphI	GGTGA	5 cut(s)	55, 558, 1570, 1745, 2096
Hpy166II	GTNNAC	4 cut(s)	224, 862, 1495, 1669
Hpy8I	GTNNAC	4 cut(s)	224, 862, 1495, 1669
HpyAV	CCTTC	6 cut(s)	151, 290, 799, 1087, 1198, 1928
HpyCH4III	ACNGT	4 cut(s)	28, 125, 312, 630
HpyCH4IV	ACGT	4 cut(s)	466, 592, 864, 880
HpyF10VI	GCNNNNNNNGC	8 cut(s)	18, 374, 418, 427, 1782, 1949, 2111, 2120
HpyF3I	CTNAG	6 cut(s)	856, 998, 1677, 1794, 1851, 2141
HpySE526I	ACGT	4 cut(s)	466, 592, 864, 880
Hsp92II	CATG	9 cut(s)	372, 377, 539, 1328, 1367, 1372, 1547, 1920, 1987
HspAI	GCGC	2 cut(s)	1774, 2103
Kpn2I	TCCGGA	1 cut(s)	714
KpnI	GGTACC	1 cut(s)	899
Ksp22I	TGATCA	1 cut(s)	571
Kzo9I	GATC	9 cut(s)	571, 910, 971, 1014, 1234, 1327, 1618, 1789, 2036
LmnI	GCTCC	5 cut(s)	41, 247, 616, 730, 1824
Lsp1109I	GCAGC	9 cut(s)	18, 21, 405, 433, 1231, 1510, 1842, 1939, 1957
LweI	GCATC	4 cut(s)	489, 544, 912, 1701
MaeI	CTAG	6 cut(s)	303, 639, 681, 927, 1430, 1782
MaeII	ACGT	4 cut(s)	466, 592, 864, 880
MaeIII	GTNAC	3 cut(s)	169, 904, 934
MalI	GATC	9 cut(s)	573, 912, 973, 1016, 1236, 1329, 1620, 1791, 2038
MbiI	CCGCTC	1 cut(s)	1681
MboI	GATC	9 cut(s)	571, 910, 971, 1014, 1234, 1327, 1618, 1789, 2036
MboII	GAAGA	6 cut(s)	156, 539, 635, 636, 859, 1135
MfeI	CAATTG	1 cut(s)	1641
MflI	RGATCY	3 cut(s)	971, 1789, 2036
MhlI	GDGCHC	5 cut(s)	432, 669, 709, 1345, 1604
MlyI	GAGTC	3 cut(s)	219, 382, 1071
MmeI	TCCRAC	2 cut(s)	454, 585
MroI	TCCGGA	1 cut(s)	714
MroXI	GAANNNNTTC	3 cut(s)	190, 819, 1386
MseI	TTAA	4 cut(s)	519, 693, 965, 1140
MslI	CAYNNNNRTG	2 cut(s)	595, 1943
MspA1I	CMGCKG	1 cut(s)	12
MspCI	CTTAAG	1 cut(s)	1139
MspI	CCGG	5 cut(s)	22, 258, 715, 1358, 1921
MspR9I	CCNGG	6 cut(s)	258, 438, 614, 900, 1151, 1922
MunI	CAATTG	1 cut(s)	1641
Mva1269I	GAATGC	1 cut(s)	1596
MvaI	CCWGG	4 cut(s)	438, 614, 900, 1151
MvnI	CGCG	1 cut(s)	1086
MwoI	GCNNNNNNNGC	8 cut(s)	18, 374, 418, 427, 1782, 1949, 2111, 2120
NciI	CCSGG	2 cut(s)	258, 1922
NdeII	GATC	9 cut(s)	571, 910, 971, 1014, 1234, 1327, 1618, 1789, 2036
NheI	GCTAGC	1 cut(s)	1781
NlaIII	CATG	9 cut(s)	372, 377, 539, 1328, 1367, 1372, 1547, 1920, 1987
NlaIV	GGNNCC	8 cut(s)	43, 249, 726, 897, 1342, 1549, 1602, 2038
NmuCI	GTSAC	1 cut(s)	934
NspI	RCATGY	3 cut(s)	372, 1367, 1920
OliI	CACNNNNGTG	1 cut(s)	595
PaeI	GCATGC	2 cut(s)	372, 1920
PagI	TCATGA	1 cut(s)	535
PaqCI	CACCTGC	1 cut(s)	1721
PceI	AGGCCT	2 cut(s)	137, 546
PciI	ACATGT	1 cut(s)	1363
PcsI	WCGNNNNNNNCGW	1 cut(s)	463
PctI	GAATGC	1 cut(s)	1596
PdmI	GAANNNNTTC	3 cut(s)	190, 819, 1386
PfeI	GAWTC	4 cut(s)	477, 815, 994, 1847
PfoI	TCCNGGA	3 cut(s)	436, 612, 1149
PleI	GAGTC	3 cut(s)	219, 382, 1070
PpsI	GAGTC	3 cut(s)	219, 382, 1070
Ppu21I	YACGTR	1 cut(s)	593
PscI	ACATGT	1 cut(s)	1363
PsiI	TTATAA	1 cut(s)	2176
Psp124BI	GAGCTC	1 cut(s)	709
Psp6I	CCWGG	4 cut(s)	436, 612, 898, 1149
PspFI	CCCAGC	1 cut(s)	1049
PspGI	CCWGG	4 cut(s)	436, 612, 898, 1149
PspN4I	GGNNCC	8 cut(s)	43, 249, 726, 897, 1342, 1549, 1602, 2038
PspOMI	GGGCCC	1 cut(s)	1600
PspPI	GGNCC	3 cut(s)	19, 1600, 1601
PstI	CTGCAG	2 cut(s)	423, 1080
PsuI	RGATCY	3 cut(s)	971, 1789, 2036
RsaI	GTAC	2 cut(s)	897, 1393
RsaNI	GTAC	2 cut(s)	896, 1392
RseI	CAYNNNNRTG	2 cut(s)	595, 1943
SacI	GAGCTC	1 cut(s)	709
SaqAI	TTAA	4 cut(s)	519, 693, 965, 1140
Sau3AI	GATC	9 cut(s)	571, 910, 971, 1014, 1234, 1327, 1618, 1789, 2036
Sau96I	GGNCC	3 cut(s)	19, 1600, 1601
SchI	GAGTC	3 cut(s)	219, 382, 1071
ScrFI	CCNGG	6 cut(s)	258, 438, 614, 900, 1151, 1922
SduI	GDGCHC	5 cut(s)	432, 669, 709, 1345, 1604
SfaNI	GCATC	4 cut(s)	489, 544, 912, 1701
SfcI	CTRYAG	2 cut(s)	419, 1076
SmiMI	CAYNNNNRTG	2 cut(s)	595, 1943
SmlI	CTYRAG	3 cut(s)	491, 708, 1139
SmoI	CTYRAG	3 cut(s)	491, 708, 1139
SphI	GCATGC	2 cut(s)	372, 1920
SseBI	AGGCCT	2 cut(s)	137, 546
SsiI	CCGC	6 cut(s)	12, 321, 835, 1084, 1373, 1681
SspI	AATATT	1 cut(s)	1247
SspMI	CTAG	6 cut(s)	303, 639, 681, 927, 1430, 1782
SstI	GAGCTC	1 cut(s)	709
StuI	AGGCCT	2 cut(s)	137, 546
StyD4I	CCNGG	6 cut(s)	256, 436, 612, 898, 1149, 1920
StyI	CCWWGG	1 cut(s)	670
TaaI	ACNGT	4 cut(s)	28, 125, 312, 630
TaiI	ACGT	4 cut(s)	469, 595, 867, 883
TaqI	TCGA	5 cut(s)	99, 204, 386, 552, 1290
TaqII	GACCGA	1 cut(s)	780
TauI	GCSGC	3 cut(s)	323, 1086, 1375
TfiI	GAWTC	4 cut(s)	477, 815, 994, 1847
Tru1I	TTAA	4 cut(s)	519, 693, 965, 1140
Tru9I	TTAA	4 cut(s)	519, 693, 965, 1140
TscAI	CASTG	5 cut(s)	289, 345, 592, 1265, 1467
TseFI	GTSAC	1 cut(s)	934
TseI	GCWGC	9 cut(s)	6, 9, 418, 421, 1219, 1523, 1855, 1952, 1970
Tsp45I	GTSAC	1 cut(s)	934
TspGWI	ACGGA	2 cut(s)	259, 2139
TspRI	CASTG	5 cut(s)	289, 345, 592, 1265, 1467
Vha464I	CTTAAG	1 cut(s)	1139
XapI	RAATTY	4 cut(s)	188, 695, 1583, 2087
XceI	RCATGY	3 cut(s)	372, 1367, 1920
XmnI	GAANNNNTTC	3 cut(s)	190, 819, 1386
XspI	CTAG	6 cut(s)	303, 639, 681, 927, 1430, 1782

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.