FvH4_4g18620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012224
Physical Location & Seq
Reverse (-)
22484860 .. 22486290
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18620.t1

Sequence Viewer

Length: 552 bp
ATGTCGAACAAGTCACCCATTTTCCCGATGCCGGAGCCCCAGCACTTCAGCGACTACGGCTTCGACCCCCAAATGGACTATTTCCAGGTCCTTGAAGAAGCCAGAAGGCACAAGCGTGACGGACCAAGATCCATAGACTCGATTCACTTCAAGCTCCAGAAGCCCATTTCTAAAGACGACCACTACTCCAAGAAGCAGCCTCACAATAAGAAGAAGCGCTGGTGGAAGAGCGCTTTGCTTTTCTTCAAATGGGGCTGGACCCACCACCACCGCCACACCCGCCCCGGCGGCCGGGAGGACGTGCACCAGGCCAGGGCCAAGGCTTTCCGGGGTTCGATTTCTGGGCCGGTTTATATCACAGAAAGCAGAAGTGGGTCGAGCACGCCGTACCGGACAGCGAGCCGGCCGTCGTCGGGGCCGCTGGCTGGGACTATGTCGCCGGTGAGCAAAGGGGAGATGGAGATTCCGTATCTGAGCCTCAGGGAGCTTAACATGGAGCAGCAACAGAGGGTGTCTACCTCATCAGCCATGCCCATTTACTTGGTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

21.17

Weight (kDa)

10.03

Isoelectric Point (pI)

71.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06840
fragaria_vesca FvH4_4g18620
malus_domestica MD13G1165100.v1.1 MD13G1165200.v1.1 MD16G1164800.v1.1
prunus_persica Prupe.1G124100_v2.0.a1
pyrus_communis pycom13g14120 pycom13g14150 pycom16g13950
rosa_chinensis RchiOBHm_Chr4g0423321
rosa_laevigata RLG00000007569
rosa_multiflora Rmu_co8278673.1_g000001 Rmu_sc0005307.1_g000001
rosa_roxburghii Rroxscaffold_5G00365500
rosa_rugosa Rorug04G0186500
rosa_samantha Rh4AG245700 Rh4BG251100 Rh4CG259500
rosa_wichuraiana Rw4G021110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 515
AciI CCGC 4 cut(s) 271, 280, 288, 419
AclWI GGATC 1 cut(s) 123
AcoI YGGCCR 2 cut(s) 289, 404
AcuI CTGAAG 1 cut(s) 31
AfaI GTAC 1 cut(s) 389
AfeI AGCGCT 2 cut(s) 218, 232
AfiI CCNNNNNNNGG 6 cut(s) 31, 73, 291, 313, 413, 425
AgsI TTSAA 3 cut(s) 95, 151, 247
AjiI CACGTC 1 cut(s) 301
AjnI CCWGG 3 cut(s) 84, 306, 311
AleI CACNNNNGTG 1 cut(s) 114
AluBI AGCT 2 cut(s) 154, 487
AluI AGCT 2 cut(s) 154, 487
Alw21I GWGCWC 2 cut(s) 306, 383
Alw44I GTGCAC 1 cut(s) 302
AlwI GGATC 1 cut(s) 123
Aor51HI AGCGCT 2 cut(s) 218, 232
AoxI GGCC 6 cut(s) 289, 309, 315, 344, 404, 416
ApaLI GTGCAC 1 cut(s) 302
ApeKI GCWGC 2 cut(s) 196, 499
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 2 cut(s) 219, 233
AspS9I GGNCC 6 cut(s) 88, 122, 258, 315, 344, 416
AsuC2I CCSGG 3 cut(s) 285, 293, 329
AsuHPI GGTGA 2 cut(s) 6, 454
AvaII GGWCC 3 cut(s) 88, 122, 258
AxyI CCTNAGG 1 cut(s) 479
BaeGI GKGCMC 1 cut(s) 306
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 306, 383
BbvI GCAGC 2 cut(s) 208, 511
BccI CCATC 1 cut(s) 451
BceAI ACGGC 3 cut(s) 73, 370, 391
BciT130I CCWGG 3 cut(s) 86, 308, 313
BcnI CCSGG 3 cut(s) 285, 293, 329
BfoI RGCGCY 2 cut(s) 220, 234
BglI GCCNNNNNGGC 1 cut(s) 288
BisI GCNGC 4 cut(s) 197, 289, 419, 500
BlsI GCNGC 4 cut(s) 198, 290, 420, 501
Bme1390I CCNGG 6 cut(s) 86, 285, 293, 308, 313, 329
Bme18I GGWCC 3 cut(s) 88, 122, 258
BmgBI CACGTC 1 cut(s) 301
BmgT120I GGNCC 6 cut(s) 88, 122, 258, 315, 344, 416
BmiI GGNNCC 3 cut(s) 36, 260, 417
BmrFI CCNGG 6 cut(s) 86, 285, 293, 308, 313, 329
BmsI GCATC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 140
BpuMI CCSGG 3 cut(s) 285, 293, 329
BsaJI CCNNGG 4 cut(s) 283, 312, 318, 328
BsaWI WCCGGW 1 cut(s) 390
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 452, 482
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 73, 291, 313, 413, 425
Bse118I RCCGGY 3 cut(s) 346, 402, 439
Bse21I CCTNAGG 1 cut(s) 479
BseBI CCWGG 3 cut(s) 86, 308, 313
BseDI CCNNGG 4 cut(s) 283, 312, 318, 328
BseLI CCNNNNNNNGG 6 cut(s) 31, 73, 291, 313, 413, 425
BseMII CTCAG 2 cut(s) 464, 493
BseSI GKGCMC 1 cut(s) 306
BseX3I CGGCCG 2 cut(s) 289, 404
BseXI GCAGC 2 cut(s) 208, 511
BseYI CCCAGC 2 cut(s) 39, 425
Bsh1285I CGRYCG 2 cut(s) 292, 407
BshFI GGCC 6 cut(s) 291, 311, 317, 346, 406, 418
BsiEI CGRYCG 2 cut(s) 292, 407
BsiHKAI GWGCWC 2 cut(s) 306, 383
BsiSI CCGG 8 cut(s) 32, 285, 292, 328, 347, 391, 403, 440
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 6 cut(s) 31, 73, 291, 313, 413, 425
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 6 cut(s) 291, 311, 317, 346, 406, 418
Bsp1286I GDGCHC 3 cut(s) 39, 306, 383
Bsp143I GATC 1 cut(s) 128
BspACI CCGC 4 cut(s) 271, 280, 288, 419
BspANI GGCC 6 cut(s) 291, 311, 317, 346, 406, 418
BspCNI CTCAG 2 cut(s) 465, 492
BspLI GGNNCC 3 cut(s) 36, 260, 417
BspPI GGATC 1 cut(s) 123
BspQI GCTCTTC 1 cut(s) 221
BsrFI RCCGGY 3 cut(s) 346, 402, 439
BssAI RCCGGY 3 cut(s) 346, 402, 439
BssECI CCNNGG 4 cut(s) 283, 312, 318, 328
BssMI GATC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 3 cut(s) 86, 308, 313
Bst6I CTCTTC 1 cut(s) 221
BstC8I GCNNGC 4 cut(s) 383, 400, 404, 423
BstDEI CTNAG 2 cut(s) 473, 479
BstH2I RGCGCY 2 cut(s) 220, 234
BstHHI GCGC 2 cut(s) 219, 233
BstKTI GATC 1 cut(s) 131
BstMBI GATC 1 cut(s) 128
BstMCI CGRYCG 2 cut(s) 292, 407
BstMWI GCNNNNNNNGC 4 cut(s) 57, 160, 279, 288
BstNI CCWGG 3 cut(s) 86, 308, 313
BstSCI CCNGG 6 cut(s) 84, 283, 291, 306, 311, 327
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 208, 511
BstX2I RGATCY 1 cut(s) 128
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 1 cut(s) 128
BstZI CGGCCG 2 cut(s) 289, 404
Bsu36I CCTNAGG 1 cut(s) 479
BsuRI GGCC 6 cut(s) 291, 311, 317, 346, 406, 418
BtrI CACGTC 1 cut(s) 301
Cac8I GCNNGC 4 cut(s) 383, 400, 404, 423
CfoI GCGC 2 cut(s) 219, 233
Cfr10I RCCGGY 3 cut(s) 346, 402, 439
Cfr13I GGNCC 6 cut(s) 88, 122, 258, 315, 344, 416
Csp6I GTAC 1 cut(s) 388
CviAII CATG 2 cut(s) 493, 529
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 2 cut(s) 473, 479
DpnI GATC 1 cut(s) 130
DpnII GATC 1 cut(s) 128
EaeI YGGCCR 2 cut(s) 289, 404
EagI CGGCCG 2 cut(s) 289, 404
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
EclXI CGGCCG 2 cut(s) 289, 404
Eco130I CCWWGG 1 cut(s) 318
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 3 cut(s) 88, 122, 258
Eco47III AGCGCT 2 cut(s) 218, 232
Eco52I CGGCCG 2 cut(s) 289, 404
Eco57I CTGAAG 1 cut(s) 31
Eco81I CCTNAGG 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 88
EcoRII CCWGG 3 cut(s) 84, 306, 311
EcoT14I CCWWGG 1 cut(s) 318
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 2 cut(s) 496, 532
FaiI YATR 5 cut(s) 134, 354, 434, 494, 530
FaqI GGGAC 1 cut(s) 442
FatI CATG 2 cut(s) 492, 528
FauI CCCGC 1 cut(s) 287
FblI GTMKAC 1 cut(s) 515
Fnu4HI GCNGC 4 cut(s) 197, 289, 419, 500
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 4 cut(s) 197, 289, 419, 500
GlaI GCGC 2 cut(s) 218, 232
GluI GCNGC 4 cut(s) 197, 289, 419, 500
GsaI CCCAGC 2 cut(s) 43, 429
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 2 cut(s) 220, 234
HaeIII GGCC 6 cut(s) 291, 311, 317, 346, 406, 418
HapII CCGG 8 cut(s) 32, 285, 292, 328, 347, 391, 403, 440
HhaI GCGC 2 cut(s) 219, 233
Hin1II CATG 2 cut(s) 496, 532
Hin6I GCGC 2 cut(s) 217, 231
HinP1I GCGC 2 cut(s) 217, 231
HinfI GANTC 3 cut(s) 137, 142, 463
HpaII CCGG 8 cut(s) 32, 285, 292, 328, 347, 391, 403, 440
HphI GGTGA 2 cut(s) 6, 454
Hpy166II GTNNAC 2 cut(s) 304, 516
Hpy188I TCNGA 1 cut(s) 474
Hpy188III TCNNGA 2 cut(s) 25, 157
Hpy8I GTNNAC 2 cut(s) 304, 516
Hpy99I CGWCG 2 cut(s) 412, 415
HpyAV CCTTC 1 cut(s) 99
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 160, 279, 288
HpyF3I CTNAG 2 cut(s) 473, 479
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 2 cut(s) 496, 532
HspAI GCGC 2 cut(s) 217, 231
KroI GCCGGC 1 cut(s) 402
KroNI GCCGGC 1 cut(s) 404
Kzo9I GATC 1 cut(s) 128
LguI GCTCTTC 1 cut(s) 221
LmnI GCTCC 4 cut(s) 34, 159, 484, 496
Lsp1109I GCAGC 2 cut(s) 208, 511
LweI GCATC 1 cut(s) 18
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 3 cut(s) 12, 116, 544
MalI GATC 1 cut(s) 130
MboI GATC 1 cut(s) 128
MboII GAAGA 4 cut(s) 107, 223, 235, 238
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 3 cut(s) 39, 306, 383
MlyI GAGTC 1 cut(s) 131
MnlI CCTC 5 cut(s) 210, 289, 488, 501, 529
MroNI GCCGGC 1 cut(s) 402
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 114
MspA1I CMGCKG 1 cut(s) 421
MspI CCGG 8 cut(s) 32, 285, 292, 328, 347, 391, 403, 440
MspR9I CCNGG 6 cut(s) 86, 285, 293, 308, 313, 329
MvaI CCWGG 3 cut(s) 86, 308, 313
MwoI GCNNNNNNNGC 4 cut(s) 57, 160, 279, 288
NaeI GCCGGC 1 cut(s) 404
NciI CCSGG 3 cut(s) 285, 293, 329
NdeII GATC 1 cut(s) 128
NgoMIV GCCGGC 1 cut(s) 402
NlaIII CATG 2 cut(s) 496, 532
NlaIV GGNNCC 3 cut(s) 36, 260, 417
NmuCI GTSAC 3 cut(s) 12, 116, 544
OliI CACNNNNGTG 1 cut(s) 114
PciSI GCTCTTC 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PdiI GCCGGC 1 cut(s) 404
PfeI GAWTC 2 cut(s) 142, 463
PflFI GACNNNGTC 1 cut(s) 433
PkrI GCNGC 4 cut(s) 198, 290, 420, 501
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PpuMI RGGWCCY 1 cut(s) 88
Psp5II RGGWCCY 1 cut(s) 88
Psp6I CCWGG 3 cut(s) 84, 306, 311
PspFI CCCAGC 2 cut(s) 39, 425
PspGI CCWGG 3 cut(s) 84, 306, 311
PspN4I GGNNCC 3 cut(s) 36, 260, 417
PspPI GGNCC 6 cut(s) 88, 122, 258, 315, 344, 416
PspPPI RGGWCCY 1 cut(s) 88
PsuI RGATCY 1 cut(s) 128
PsyI GACNNNGTC 1 cut(s) 433
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 1 cut(s) 114
SapI GCTCTTC 1 cut(s) 221
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 4 cut(s) 197, 289, 419, 500
Sau3AI GATC 1 cut(s) 128
Sau96I GGNCC 6 cut(s) 88, 122, 258, 315, 344, 416
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 6 cut(s) 86, 285, 293, 308, 313, 329
SduI GDGCHC 3 cut(s) 39, 306, 383
SetI ASST 5 cut(s) 90, 156, 303, 489, 521
SfaNI GCATC 1 cut(s) 18
SgrAI CRCCGGYG 1 cut(s) 439
SinI GGWCC 3 cut(s) 88, 122, 258
SmiMI CAYNNNNRTG 1 cut(s) 114
SsiI CCGC 4 cut(s) 271, 280, 288, 419
StyD4I CCNGG 6 cut(s) 84, 283, 291, 306, 311, 327
StyI CCWWGG 1 cut(s) 318
TaiI ACGT 1 cut(s) 303
TaqI TCGA 5 cut(s) 5, 63, 140, 335, 377
TauI GCSGC 2 cut(s) 291, 421
TfiI GAWTC 2 cut(s) 142, 463
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseFI GTSAC 3 cut(s) 12, 116, 544
TseI GCWGC 2 cut(s) 196, 499
Tsp45I GTSAC 3 cut(s) 12, 116, 544
TspGWI ACGGA 2 cut(s) 135, 456
Tth111I GACNNNGTC 1 cut(s) 433
VneI GTGCAC 1 cut(s) 302
VpaK11BI GGWCC 3 cut(s) 88, 122, 258
XmiI GTMKAC 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.