MD13G1165200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012224
Physical Location & Seq
Forward (+)
13059468 .. 13060439
972 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1165200.v1.1.491

Sequence Viewer

Length: 567 bp
ATGTCCAACAAGTCACCAATCTTTCCTATGCCACAACCCCAACACTTCAGCGACTACGGCTTCGACCCCCAAGTCGACTACTTTCAGGTTTTGGAGGAAGCAAGGAGGCACAAGCGGGAGAAGACGAGATCCATAGACTCCAAACACTTCAAGCTCCAAAAGCGCATCTCAAAAGACGACTCCAGAAAGCCCCACCACAACAAAACCAATAAGAAGCGCTGGTGGAAAAGCGCTATGCTTTTCTTCAAATGGAACAAGCTGACACCTCACCACCACCACCACCATGTCTCCGCCATCCGCGGCGGCGATGAAGATGTTCACCACGCCAGGGCCAAAGCCTTCCGGGGATCGATTTGTGGGCCGGTTTATATCACTGAGAGCAGAAGCGGGTCGTGCACTCCTTGCCTGAGTACTAGCCGGCCGTCGTCTCGGGCGCTCGCCGGGACTATGTCTCCGGCGAGTAAGGATGAGATGGGCATTCCTTACCTGAACCTGAGGGAGCTTAACATGGAGCAGCAGCAACATAGGAACTCTACCTCTGCCGTGCCTATATATTTGGTTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

21.77

Weight (kDa)

9.97

Isoelectric Point (pI)

70.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06840
fragaria_vesca FvH4_4g18620
malus_domestica MD13G1165100.v1.1 MD13G1165200.v1.1 MD16G1164800.v1.1
prunus_persica Prupe.1G124100_v2.0.a1
pyrus_communis pycom13g14120 pycom13g14150 pycom16g13950
rosa_chinensis RchiOBHm_Chr4g0423321
rosa_laevigata RLG00000007569
rosa_multiflora Rmu_co8278673.1_g000001 Rmu_sc0005307.1_g000001
rosa_roxburghii Rroxscaffold_5G00365500
rosa_rugosa Rorug04G0186500
rosa_samantha Rh4AG245700 Rh4BG251100 Rh4CG259500
rosa_wichuraiana Rw4G021110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
AccI GTMKAC 1 cut(s) 75
AccII CGCG 1 cut(s) 300
AciI CCGC 6 cut(s) 115, 291, 298, 300, 303, 387
AclWI GGATC 2 cut(s) 123, 355
AcoI YGGCCR 1 cut(s) 419
AcuI CTGAAG 1 cut(s) 31
AfaI GTAC 1 cut(s) 412
AfeI AGCGCT 2 cut(s) 218, 232
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 2 cut(s) 151, 247
AjnI CCWGG 1 cut(s) 326
AluBI AGCT 3 cut(s) 154, 259, 502
AluI AGCT 3 cut(s) 154, 259, 502
Alw21I GWGCWC 1 cut(s) 398
Alw26I GTCTC 3 cut(s) 292, 432, 456
Alw44I GTGCAC 1 cut(s) 394
AlwI GGATC 2 cut(s) 123, 355
Ama87I CYCGRG 1 cut(s) 429
Aor51HI AGCGCT 2 cut(s) 218, 232
AoxI GGCC 3 cut(s) 330, 359, 419
ApaLI GTGCAC 1 cut(s) 394
ApeKI GCWGC 2 cut(s) 514, 517
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp700I GAANNNNTTC 1 cut(s) 315
AspLEI GCGC 4 cut(s) 165, 219, 233, 436
AspS9I GGNCC 2 cut(s) 330, 359
AsuC2I CCSGG 2 cut(s) 344, 442
AsuHPI GGTGA 3 cut(s) 6, 260, 311
AvaI CYCGRG 1 cut(s) 429
AxyI CCTNAGG 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 398
BbvI GCAGC 2 cut(s) 526, 529
BccI CCATC 2 cut(s) 302, 466
BceAI ACGGC 3 cut(s) 73, 406, 527
BciT130I CCWGG 1 cut(s) 328
BcnI CCSGG 2 cut(s) 344, 442
BcoDI GTCTC 3 cut(s) 292, 432, 456
BfaI CTAG 1 cut(s) 414
BfoI RGCGCY 3 cut(s) 220, 234, 437
BisI GCNGC 4 cut(s) 301, 304, 515, 518
BlsI GCNGC 4 cut(s) 302, 305, 516, 519
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 3 cut(s) 328, 344, 442
BmeT110I CYCGRG 1 cut(s) 429
BmgT120I GGNCC 2 cut(s) 330, 359
BmrFI CCNGG 3 cut(s) 328, 344, 442
BmsI GCATC 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 128
BpmI CTGGAG 1 cut(s) 166
BpuMI CCSGG 2 cut(s) 344, 442
Bsa29I ATCGAT 1 cut(s) 350
BsaJI CCNNGG 3 cut(s) 298, 327, 343
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 436, 466
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse118I RCCGGY 2 cut(s) 361, 417
Bse21I CCTNAGG 1 cut(s) 494
BseBI CCWGG 1 cut(s) 328
BseCI ATCGAT 1 cut(s) 350
BseDI CCNNGG 3 cut(s) 298, 327, 343
BseGI GGATG 2 cut(s) 294, 472
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMII CTCAG 3 cut(s) 366, 398, 485
BseSI GKGCMC 1 cut(s) 398
BseX3I CGGCCG 1 cut(s) 419
BseXI GCAGC 2 cut(s) 526, 529
Bsh1236I CGCG 1 cut(s) 300
Bsh1285I CGRYCG 1 cut(s) 422
BshFI GGCC 3 cut(s) 332, 361, 421
BshVI ATCGAT 1 cut(s) 350
BsiEI CGRYCG 1 cut(s) 422
BsiHKAI GWGCWC 1 cut(s) 398
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 5 cut(s) 343, 362, 418, 441, 455
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 3 cut(s) 292, 432, 456
BsmBI CGTCTC 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 457
BsmI GAATGC 1 cut(s) 477
BsnI GGCC 3 cut(s) 332, 361, 421
BsoBI CYCGRG 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 398
Bsp143I GATC 2 cut(s) 128, 347
BspACI CCGC 6 cut(s) 115, 291, 298, 300, 303, 387
BspANI GGCC 3 cut(s) 332, 361, 421
BspCNI CTCAG 3 cut(s) 367, 399, 486
BspDI ATCGAT 1 cut(s) 350
BspFNI CGCG 1 cut(s) 300
BspPI GGATC 2 cut(s) 123, 355
BsrFI RCCGGY 2 cut(s) 361, 417
BssAI RCCGGY 2 cut(s) 361, 417
BssECI CCNNGG 3 cut(s) 298, 327, 343
BssMI GATC 2 cut(s) 128, 347
Bst2UI CCWGG 1 cut(s) 328
BstAPI GCANNNNNTGC 1 cut(s) 402
BstC8I GCNNGC 2 cut(s) 419, 438
BstDEI CTNAG 3 cut(s) 375, 407, 494
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 2 cut(s) 294, 472
BstFNI CGCG 1 cut(s) 300
BstH2I RGCGCY 3 cut(s) 220, 234, 437
BstHHI GCGC 4 cut(s) 165, 219, 233, 436
BstKTI GATC 2 cut(s) 131, 350
BstMAI GTCTC 3 cut(s) 292, 432, 456
BstMBI GATC 2 cut(s) 128, 347
BstMCI CGRYCG 1 cut(s) 422
BstMWI GCNNNNNNNGC 4 cut(s) 57, 160, 393, 402
BstNI CCWGG 1 cut(s) 328
BstSCI CCNGG 3 cut(s) 326, 342, 440
BstSLI GKGCMC 1 cut(s) 398
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 526, 529
BstV2I GAAGAC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
BstZI CGGCCG 1 cut(s) 419
Bsu15I ATCGAT 1 cut(s) 350
Bsu36I CCTNAGG 1 cut(s) 494
BsuRI GGCC 3 cut(s) 332, 361, 421
BsuTUI ATCGAT 1 cut(s) 350
BtgI CCRYGG 1 cut(s) 298
BtgZI GCGATG 1 cut(s) 321
BtsCI GGATG 2 cut(s) 294, 472
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 2 cut(s) 419, 438
CfoI GCGC 4 cut(s) 165, 219, 233, 436
Cfr10I RCCGGY 2 cut(s) 361, 417
Cfr13I GGNCC 2 cut(s) 330, 359
Cfr42I CCGCGG 1 cut(s) 301
ClaI ATCGAT 1 cut(s) 350
Csp6I GTAC 1 cut(s) 411
CviAII CATG 2 cut(s) 284, 508
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 3 cut(s) 375, 407, 494
DpnI GATC 2 cut(s) 130, 349
DpnII GATC 2 cut(s) 128, 347
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 419
EagI CGGCCG 1 cut(s) 419
EciI GGCGGA 1 cut(s) 280
EclXI CGGCCG 1 cut(s) 419
Eco47III AGCGCT 2 cut(s) 218, 232
Eco52I CGGCCG 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 31
Eco81I CCTNAGG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 429
EcoRII CCWGG 1 cut(s) 326
Esp3I CGTCTC 1 cut(s) 432
FaeI CATG 2 cut(s) 287, 511
FaqI GGGAC 1 cut(s) 457
FatI CATG 2 cut(s) 283, 507
FauI CCCGC 2 cut(s) 108, 380
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 4 cut(s) 301, 304, 515, 518
FokI GGATG 2 cut(s) 281, 479
Fsp4HI GCNGC 4 cut(s) 301, 304, 515, 518
FspBI CTAG 1 cut(s) 414
GlaI GCGC 4 cut(s) 164, 218, 232, 435
GluI GCNGC 4 cut(s) 301, 304, 515, 518
GsuI CTGGAG 1 cut(s) 166
HaeII RGCGCY 3 cut(s) 220, 234, 437
HaeIII GGCC 3 cut(s) 332, 361, 421
HapII CCGG 5 cut(s) 343, 362, 418, 441, 455
HhaI GCGC 4 cut(s) 165, 219, 233, 436
Hin1II CATG 2 cut(s) 287, 511
Hin6I GCGC 4 cut(s) 163, 217, 231, 434
HinP1I GCGC 4 cut(s) 163, 217, 231, 434
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 2 cut(s) 137, 179
HpaII CCGG 5 cut(s) 343, 362, 418, 441, 455
HphI GGTGA 3 cut(s) 6, 260, 311
Hpy166II GTNNAC 3 cut(s) 76, 319, 396
Hpy188III TCNNGA 1 cut(s) 183
Hpy8I GTNNAC 3 cut(s) 76, 319, 396
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 1 cut(s) 349
HpyCH4V TGCA 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 160, 393, 402
HpyF3I CTNAG 3 cut(s) 375, 407, 494
Hsp92II CATG 2 cut(s) 287, 511
HspAI GCGC 4 cut(s) 163, 217, 231, 434
KroI GCCGGC 1 cut(s) 417
KroNI GCCGGC 1 cut(s) 419
KspI CCGCGG 1 cut(s) 301
Kzo9I GATC 2 cut(s) 128, 347
LmnI GCTCC 3 cut(s) 159, 499, 511
Lsp1109I GCAGC 2 cut(s) 526, 529
LweI GCATC 1 cut(s) 174
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 2 cut(s) 12, 559
MalI GATC 2 cut(s) 130, 349
MboI GATC 2 cut(s) 128, 347
MboII GAAGA 3 cut(s) 133, 235, 323
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 1 cut(s) 398
MlyI GAGTC 2 cut(s) 131, 173
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 5 cut(s) 88, 99, 276, 489, 547
MroNI GCCGGC 1 cut(s) 417
MroXI GAANNNNTTC 1 cut(s) 315
MseI TTAA 2 cut(s) 504, 565
MslI CAYNNNNRTG 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 300
MspI CCGG 5 cut(s) 343, 362, 418, 441, 455
MspR9I CCNGG 3 cut(s) 328, 344, 442
Mva1269I GAATGC 1 cut(s) 477
MvaI CCWGG 1 cut(s) 328
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 4 cut(s) 57, 160, 393, 402
NaeI GCCGGC 1 cut(s) 419
NciI CCSGG 2 cut(s) 344, 442
NdeII GATC 2 cut(s) 128, 347
NgoMIV GCCGGC 1 cut(s) 417
NlaIII CATG 2 cut(s) 287, 511
NmuCI GTSAC 1 cut(s) 12
PctI GAATGC 1 cut(s) 477
PdiI GCCGGC 1 cut(s) 419
PdmI GAANNNNTTC 1 cut(s) 315
PflFI GACNNNGTC 1 cut(s) 448
PkrI GCNGC 4 cut(s) 302, 305, 516, 519
PleI GAGTC 2 cut(s) 131, 173
PpsI GAGTC 2 cut(s) 131, 173
Psp6I CCWGG 1 cut(s) 326
PspGI CCWGG 1 cut(s) 326
PspPI GGNCC 2 cut(s) 330, 359
PsuI RGATCY 1 cut(s) 128
PsyI GACNNNGTC 1 cut(s) 448
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 1 cut(s) 282
SacII CCGCGG 1 cut(s) 301
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 2 cut(s) 504, 565
SatI GCNGC 4 cut(s) 301, 304, 515, 518
Sau3AI GATC 2 cut(s) 128, 347
Sau96I GGNCC 2 cut(s) 330, 359
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 2 cut(s) 131, 173
ScrFI CCNGG 3 cut(s) 328, 344, 442
SduI GDGCHC 1 cut(s) 398
SetI ASST 8 cut(s) 90, 156, 261, 268, 489, 495, 504, 539
SfaNI GCATC 1 cut(s) 174
Sfr303I CCGCGG 1 cut(s) 301
SgrBI CCGCGG 1 cut(s) 301
SmiMI CAYNNNNRTG 1 cut(s) 282
SsiI CCGC 6 cut(s) 115, 291, 298, 300, 303, 387
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 3 cut(s) 326, 342, 440
TaqI TCGA 3 cut(s) 63, 75, 350
TatI WGTACW 1 cut(s) 410
TauI GCSGC 2 cut(s) 303, 306
Tru1I TTAA 2 cut(s) 504, 565
Tru9I TTAA 2 cut(s) 504, 565
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 1 cut(s) 12
TseI GCWGC 2 cut(s) 514, 517
Tsp45I GTSAC 1 cut(s) 12
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 1 cut(s) 379
Tth111I GACNNNGTC 1 cut(s) 448
VneI GTGCAC 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 1 cut(s) 414
ZrmI AGTACT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.