Rw4G021110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012224
Physical Location & Seq
Reverse (-)
45605324 .. 45605983
660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G021110.1

Sequence Viewer

Length: 552 bp
ATGTCGAACAAGTCACCCATTTTTCCGATGCCGGAGCCCCAACACTTCAGCGACTACGGCTTTGACCCCCAAATCGACTATTTCCAGAAGCTAGAAGGCAAGCGTGACGGAACGAGATCCATAGACTCGATTCACTTCAAGCTCCAGAAGCCCATTTCCAAGGACGACCACTACTCCAAGAAGCCTCACAAGAAGAAGCGCTGGTGGAAAAGCGCTTTGCTTTTCTTCAAATGGGGCTGGACCCACCACCACCACCACGACTCGGCCCACCGCCACAACCGCCGTGGTGGCCGGGAGGACGTGCACCAGGCCAGGGCCAAGGTTTTCCGGGCTTCCATTTCCGGGCCGGTTTATATCACAGAAAGCAGGAGCGGGTCGAGCACACCGTACCGGACAGCGAGCCGGCCGTCGTCGGGACCGCTGGCCGGGACTATGTCGCCGGTGAGTAAGGGAGAGATGGAGATTCCGTATCTGAGCCTGAGAGAGCTTAACATGGAGCAGCAACAGAGGGTGTCTACCTCGTCAGCCATGCCCATTTACTTGGTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

21.15

Weight (kDa)

9.99

Isoelectric Point (pI)

63.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06840
fragaria_vesca FvH4_4g18620
malus_domestica MD13G1165100.v1.1 MD13G1165200.v1.1 MD16G1164800.v1.1
prunus_persica Prupe.1G124100_v2.0.a1
pyrus_communis pycom13g14120 pycom13g14150 pycom16g13950
rosa_chinensis RchiOBHm_Chr4g0423321
rosa_laevigata RLG00000007569
rosa_multiflora Rmu_co8278673.1_g000001 Rmu_sc0005307.1_g000001
rosa_roxburghii Rroxscaffold_5G00365500
rosa_rugosa Rorug04G0186500
rosa_samantha Rh4AG245700 Rh4BG251100 Rh4CG259500
rosa_wichuraiana Rw4G021110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 372
AccI GTMKAC 1 cut(s) 515
AciI CCGC 4 cut(s) 271, 280, 372, 419
AclWI GGATC 1 cut(s) 111
AcoI YGGCCR 3 cut(s) 289, 404, 423
AcuI CTGAAG 1 cut(s) 31
AfaI GTAC 1 cut(s) 389
AfeI AGCGCT 2 cut(s) 200, 214
AfiI CCNNNNNNNGG 5 cut(s) 262, 313, 342, 413, 425
AgsI TTSAA 2 cut(s) 139, 229
AjiI CACGTC 1 cut(s) 301
AjnI CCWGG 2 cut(s) 306, 311
AluBI AGCT 3 cut(s) 91, 142, 487
AluI AGCT 3 cut(s) 91, 142, 487
Alw21I GWGCWC 2 cut(s) 306, 383
Alw44I GTGCAC 1 cut(s) 302
AlwI GGATC 1 cut(s) 111
Aor51HI AGCGCT 2 cut(s) 200, 214
AoxI GGCC 7 cut(s) 264, 289, 309, 315, 344, 404, 423
ApaLI GTGCAC 1 cut(s) 302
ApeKI GCWGC 1 cut(s) 499
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 2 cut(s) 201, 215
AspS9I GGNCC 5 cut(s) 240, 265, 315, 344, 416
AsuC2I CCSGG 4 cut(s) 293, 329, 343, 427
AsuHPI GGTGA 2 cut(s) 6, 454
AvaII GGWCC 2 cut(s) 240, 416
BaeGI GKGCMC 1 cut(s) 306
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 306, 383
BbvI GCAGC 1 cut(s) 511
BccI CCATC 1 cut(s) 451
BceAI ACGGC 3 cut(s) 73, 267, 391
BciT130I CCWGG 2 cut(s) 308, 313
BcnI CCSGG 4 cut(s) 293, 329, 343, 427
BfaI CTAG 1 cut(s) 92
BfoI RGCGCY 2 cut(s) 202, 216
BglI GCCNNNNNGGC 1 cut(s) 288
BisI GCNGC 1 cut(s) 500
BlsI GCNGC 1 cut(s) 501
Bme1390I CCNGG 6 cut(s) 293, 308, 313, 329, 343, 427
Bme18I GGWCC 2 cut(s) 240, 416
BmgBI CACGTC 1 cut(s) 301
BmgT120I GGNCC 5 cut(s) 240, 265, 315, 344, 416
BmiI GGNNCC 3 cut(s) 36, 242, 417
BmrFI CCNGG 6 cut(s) 293, 308, 313, 329, 343, 427
BmsI GCATC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 128
BpuMI CCSGG 4 cut(s) 293, 329, 343, 427
BsaJI CCNNGG 4 cut(s) 159, 283, 312, 318
BsaWI WCCGGW 1 cut(s) 390
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 452, 482
Bsc4I CCNNNNNNNGG 5 cut(s) 262, 313, 342, 413, 425
Bse118I RCCGGY 3 cut(s) 346, 402, 439
BseBI CCWGG 2 cut(s) 308, 313
BseDI CCNNGG 4 cut(s) 159, 283, 312, 318
BseLI CCNNNNNNNGG 5 cut(s) 262, 313, 342, 413, 425
BseMII CTCAG 2 cut(s) 464, 470
BseSI GKGCMC 1 cut(s) 306
BseX3I CGGCCG 1 cut(s) 404
BseXI GCAGC 1 cut(s) 511
Bsh1285I CGRYCG 1 cut(s) 407
BshFI GGCC 7 cut(s) 266, 291, 311, 317, 346, 406, 425
BsiEI CGRYCG 1 cut(s) 407
BsiHKAI GWGCWC 2 cut(s) 306, 383
BsiSI CCGG 9 cut(s) 32, 292, 328, 342, 347, 391, 403, 426, 440
BslFI GGGAC 2 cut(s) 429, 442
BslI CCNNNNNNNGG 5 cut(s) 262, 313, 342, 413, 425
BsmFI GGGAC 2 cut(s) 429, 442
BsnI GGCC 7 cut(s) 266, 291, 311, 317, 346, 406, 425
Bsp1286I GDGCHC 3 cut(s) 39, 306, 383
Bsp143I GATC 1 cut(s) 116
BspACI CCGC 4 cut(s) 271, 280, 372, 419
BspANI GGCC 7 cut(s) 266, 291, 311, 317, 346, 406, 425
BspCNI CTCAG 2 cut(s) 465, 471
BspLI GGNNCC 3 cut(s) 36, 242, 417
BspPI GGATC 1 cut(s) 111
BsrBI CCGCTC 1 cut(s) 372
BsrFI RCCGGY 3 cut(s) 346, 402, 439
BssAI RCCGGY 3 cut(s) 346, 402, 439
BssECI CCNNGG 4 cut(s) 159, 283, 312, 318
BssMI GATC 1 cut(s) 116
BssT1I CCWWGG 2 cut(s) 159, 318
Bst2UI CCWGG 2 cut(s) 308, 313
Bst4CI ACNGT 1 cut(s) 387
BstC8I GCNNGC 4 cut(s) 101, 400, 404, 423
BstDEI CTNAG 2 cut(s) 473, 479
BstDSI CCRYGG 1 cut(s) 283
BstH2I RGCGCY 2 cut(s) 202, 216
BstHHI GCGC 2 cut(s) 201, 215
BstKTI GATC 1 cut(s) 119
BstMBI GATC 1 cut(s) 116
BstMCI CGRYCG 1 cut(s) 407
BstMWI GCNNNNNNNGC 5 cut(s) 57, 148, 279, 288, 378
BstNI CCWGG 2 cut(s) 308, 313
BstSCI CCNGG 6 cut(s) 291, 306, 311, 327, 341, 425
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 511
BstX2I RGATCY 1 cut(s) 116
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 1 cut(s) 116
BstZI CGGCCG 1 cut(s) 404
BsuRI GGCC 7 cut(s) 266, 291, 311, 317, 346, 406, 425
BtgI CCRYGG 1 cut(s) 283
BtrI CACGTC 1 cut(s) 301
Cac8I GCNNGC 4 cut(s) 101, 400, 404, 423
CfoI GCGC 2 cut(s) 201, 215
Cfr10I RCCGGY 3 cut(s) 346, 402, 439
Cfr13I GGNCC 5 cut(s) 240, 265, 315, 344, 416
Csp6I GTAC 1 cut(s) 388
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 2 cut(s) 493, 529
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 2 cut(s) 473, 479
DpnI GATC 1 cut(s) 118
DpnII GATC 1 cut(s) 116
EaeI YGGCCR 3 cut(s) 289, 404, 423
EagI CGGCCG 1 cut(s) 404
EclXI CGGCCG 1 cut(s) 404
Eco130I CCWWGG 2 cut(s) 159, 318
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 2 cut(s) 240, 416
Eco47III AGCGCT 2 cut(s) 200, 214
Eco52I CGGCCG 1 cut(s) 404
Eco57I CTGAAG 1 cut(s) 31
EcoRII CCWGG 2 cut(s) 306, 311
EcoT14I CCWWGG 2 cut(s) 159, 318
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 2 cut(s) 159, 318
FaeI CATG 2 cut(s) 496, 532
FaiI YATR 5 cut(s) 122, 354, 434, 494, 530
FaqI GGGAC 2 cut(s) 429, 442
FatI CATG 2 cut(s) 492, 528
FauI CCCGC 1 cut(s) 365
FblI GTMKAC 1 cut(s) 515
Fnu4HI GCNGC 1 cut(s) 500
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 1 cut(s) 500
FspBI CTAG 1 cut(s) 92
GlaI GCGC 2 cut(s) 200, 214
GluI GCNGC 1 cut(s) 500
GsuI CTGGAG 1 cut(s) 128
HaeII RGCGCY 2 cut(s) 202, 216
HaeIII GGCC 7 cut(s) 266, 291, 311, 317, 346, 406, 425
HapII CCGG 9 cut(s) 32, 292, 328, 342, 347, 391, 403, 426, 440
HhaI GCGC 2 cut(s) 201, 215
Hin1II CATG 2 cut(s) 496, 532
Hin6I GCGC 2 cut(s) 199, 213
HinP1I GCGC 2 cut(s) 199, 213
HinfI GANTC 4 cut(s) 125, 130, 260, 463
HpaII CCGG 9 cut(s) 32, 292, 328, 342, 347, 391, 403, 426, 440
HphI GGTGA 2 cut(s) 6, 454
Hpy166II GTNNAC 2 cut(s) 304, 516
Hpy188I TCNGA 2 cut(s) 27, 474
Hpy188III TCNNGA 3 cut(s) 85, 145, 414
Hpy8I GTNNAC 2 cut(s) 304, 516
Hpy99I CGWCG 2 cut(s) 412, 415
HpyAV CCTTC 1 cut(s) 89
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 148, 279, 288, 378
HpyF3I CTNAG 2 cut(s) 473, 479
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 2 cut(s) 496, 532
HspAI GCGC 2 cut(s) 199, 213
KroI GCCGGC 1 cut(s) 402
KroNI GCCGGC 1 cut(s) 404
Kzo9I GATC 1 cut(s) 116
LmnI GCTCC 4 cut(s) 34, 147, 369, 496
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 1 cut(s) 18
MaeI CTAG 1 cut(s) 92
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 3 cut(s) 12, 104, 544
MalI GATC 1 cut(s) 118
MbiI CCGCTC 1 cut(s) 372
MboI GATC 1 cut(s) 116
MboII GAAGA 2 cut(s) 205, 217
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 3 cut(s) 39, 306, 383
MlyI GAGTC 2 cut(s) 119, 254
MnlI CCTC 4 cut(s) 195, 289, 501, 529
MroNI GCCGGC 1 cut(s) 402
MseI TTAA 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 421
MspI CCGG 9 cut(s) 32, 292, 328, 342, 347, 391, 403, 426, 440
MspR9I CCNGG 6 cut(s) 293, 308, 313, 329, 343, 427
MvaI CCWGG 2 cut(s) 308, 313
MwoI GCNNNNNNNGC 5 cut(s) 57, 148, 279, 288, 378
NaeI GCCGGC 1 cut(s) 404
NciI CCSGG 4 cut(s) 293, 329, 343, 427
NdeII GATC 1 cut(s) 116
NgoMIV GCCGGC 1 cut(s) 402
NlaIII CATG 2 cut(s) 496, 532
NlaIV GGNNCC 3 cut(s) 36, 242, 417
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 2 cut(s) 12, 104
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PdiI GCCGGC 1 cut(s) 404
PfeI GAWTC 2 cut(s) 130, 463
PflFI GACNNNGTC 1 cut(s) 433
PkrI GCNGC 1 cut(s) 501
PleI GAGTC 2 cut(s) 119, 254
PpsI GAGTC 2 cut(s) 119, 254
Psp6I CCWGG 2 cut(s) 306, 311
PspGI CCWGG 2 cut(s) 306, 311
PspN4I GGNNCC 3 cut(s) 36, 242, 417
PspPI GGNCC 5 cut(s) 240, 265, 315, 344, 416
PsuI RGATCY 1 cut(s) 116
PsyI GACNNNGTC 1 cut(s) 433
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 1 cut(s) 500
Sau3AI GATC 1 cut(s) 116
Sau96I GGNCC 5 cut(s) 240, 265, 315, 344, 416
SchI GAGTC 2 cut(s) 119, 254
ScrFI CCNGG 6 cut(s) 293, 308, 313, 329, 343, 427
SduI GDGCHC 3 cut(s) 39, 306, 383
SetI ASST 6 cut(s) 93, 144, 303, 324, 489, 521
SfaNI GCATC 1 cut(s) 18
SgrAI CRCCGGYG 1 cut(s) 439
SinI GGWCC 2 cut(s) 240, 416
SsiI CCGC 4 cut(s) 271, 280, 372, 419
SspMI CTAG 1 cut(s) 92
StyD4I CCNGG 6 cut(s) 291, 306, 311, 327, 341, 425
StyI CCWWGG 2 cut(s) 159, 318
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 1 cut(s) 303
TaqI TCGA 4 cut(s) 5, 75, 128, 377
TfiI GAWTC 2 cut(s) 130, 463
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseFI GTSAC 2 cut(s) 12, 104
TseI GCWGC 1 cut(s) 499
Tsp45I GTSAC 2 cut(s) 12, 104
TspGWI ACGGA 2 cut(s) 123, 456
Tth111I GACNNNGTC 1 cut(s) 433
VneI GTGCAC 1 cut(s) 302
VpaK11BI GGWCC 2 cut(s) 240, 416
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 515
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.