MD13G1165100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012224
Physical Location & Seq
Forward (+)
13050796 .. 13051278
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1165100.v1.1.491

Sequence Viewer

Length: 483 bp
ATGGGAAGTGGGAGGGTAGAGAAGCCGAGTCCGACGATGAGATCCATAGACTCCAAACATTTCAAGCTTCAAAAGCCCATCTCAAAAGATGACTCCAAAAAGCCCCACCACAACAAAACTAAGAAGAAGCGATGGTGGAAAAGCGCTCTTCTTTTCTTCAAATGGAACAAGCTAACACCCCACCACCACTACCACCGTGTCTCCGCCATCCGCGGCGGTGATGAAAATGTTCAACACCAGGCCAGAGCCAAAGCCTTTGGGGCATCCATTTTTGGGCCGGTTTATATCACTGAGAGTATAAGCTGGTCGTGCATGCCTTACCTGAGTACTAGCCGGCCGCCGTCTGGGCCGCTCGCTGGTACTATGCCTACGGCGAGTAAGGATGAGATGGGCATTCCTTACTTGAACCTGAGAGAGCTTAACATGGAGCAACAGCAACAGAGGAACTCTACCTCTGCCATGCCTATATATTTGGTTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

18.25

Weight (kDa)

10.12

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06840
fragaria_vesca FvH4_4g18620
malus_domestica MD13G1165100.v1.1 MD13G1165200.v1.1 MD16G1164800.v1.1
prunus_persica Prupe.1G124100_v2.0.a1
pyrus_communis pycom13g14120 pycom13g14150 pycom16g13950
rosa_chinensis RchiOBHm_Chr4g0423321
rosa_laevigata RLG00000007569
rosa_multiflora Rmu_co8278673.1_g000001 Rmu_sc0005307.1_g000001
rosa_roxburghii Rroxscaffold_5G00365500
rosa_rugosa Rorug04G0186500
rosa_samantha Rh4AG245700 Rh4BG251100 Rh4CG259500
rosa_wichuraiana Rw4G021110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 352
AccII CGCG 1 cut(s) 213
AciI CCGC 6 cut(s) 204, 211, 213, 216, 338, 350
AclWI GGATC 1 cut(s) 36
AcoI YGGCCR 1 cut(s) 335
AfaI GTAC 2 cut(s) 328, 361
AfeI AGCGCT 1 cut(s) 145
AfiI CCNNNNNNNGG 3 cut(s) 273, 344, 356
AgsI TTSAA 5 cut(s) 64, 71, 160, 233, 406
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 4 cut(s) 67, 172, 303, 418
AluI AGCT 4 cut(s) 67, 172, 303, 418
Alw26I GTCTC 1 cut(s) 205
AlwI GGATC 1 cut(s) 36
Aor51HI AGCGCT 1 cut(s) 145
AoxI GGCC 4 cut(s) 240, 275, 335, 347
Asp700I GAANNNNTTC 1 cut(s) 228
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 2 cut(s) 275, 347
AsuHPI GGTGA 1 cut(s) 230
BccI CCATC 4 cut(s) 86, 126, 215, 382
BceAI ACGGC 2 cut(s) 325, 387
BciT130I CCWGG 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 1 cut(s) 330
BfoI RGCGCY 1 cut(s) 147
BglI GCCNNNNNGGC 2 cut(s) 260, 346
BisI GCNGC 3 cut(s) 214, 338, 350
BlsI GCNGC 3 cut(s) 215, 339, 351
BmcAI AGTACT 1 cut(s) 328
Bme1390I CCNGG 1 cut(s) 239
BmgT120I GGNCC 2 cut(s) 275, 347
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 3 cut(s) 273, 344, 356
Bse118I RCCGGY 2 cut(s) 277, 333
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 211
BseGI GGATG 3 cut(s) 207, 263, 388
BseLI CCNNNNNNNGG 3 cut(s) 273, 344, 356
BseMII CTCAG 3 cut(s) 282, 314, 401
BseX3I CGGCCG 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 213
Bsh1285I CGRYCG 1 cut(s) 338
BshFI GGCC 4 cut(s) 242, 277, 337, 349
BsiEI CGRYCG 1 cut(s) 338
BsiSI CCGG 2 cut(s) 278, 334
BslI CCNNNNNNNGG 3 cut(s) 273, 344, 356
BsmAI GTCTC 1 cut(s) 205
BsmI GAATGC 1 cut(s) 393
BsnI GGCC 4 cut(s) 242, 277, 337, 349
Bsp143I GATC 1 cut(s) 41
BspACI CCGC 6 cut(s) 204, 211, 213, 216, 338, 350
BspANI GGCC 4 cut(s) 242, 277, 337, 349
BspCNI CTCAG 3 cut(s) 283, 315, 402
BspFNI CGCG 1 cut(s) 213
BspPI GGATC 1 cut(s) 36
BspQI GCTCTTC 1 cut(s) 153
BsrBI CCGCTC 1 cut(s) 352
BsrFI RCCGGY 2 cut(s) 277, 333
BssAI RCCGGY 2 cut(s) 277, 333
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 1 cut(s) 41
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 197
Bst6I CTCTTC 1 cut(s) 153
BstC8I GCNNGC 3 cut(s) 314, 335, 354
BstDEI CTNAG 4 cut(s) 120, 291, 323, 410
BstDSI CCRYGG 1 cut(s) 211
BstF5I GGATG 3 cut(s) 207, 263, 388
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 1 cut(s) 44
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 1 cut(s) 41
BstMCI CGRYCG 1 cut(s) 338
BstMWI GCNNNNNNNGC 4 cut(s) 73, 260, 309, 346
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 1 cut(s) 316
BstSCI CCNGG 1 cut(s) 237
BstUI CGCG 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
BstZI CGGCCG 1 cut(s) 335
BsuRI GGCC 4 cut(s) 242, 277, 337, 349
BtgI CCRYGG 1 cut(s) 211
BtgZI GCGATG 1 cut(s) 145
BtsCI GGATG 3 cut(s) 207, 263, 388
BtsIMutI CAGTG 1 cut(s) 288
Cac8I GCNNGC 3 cut(s) 314, 335, 354
CfoI GCGC 1 cut(s) 146
Cfr10I RCCGGY 2 cut(s) 277, 333
Cfr13I GGNCC 2 cut(s) 275, 347
Cfr42I CCGCGG 1 cut(s) 214
Csp6I GTAC 2 cut(s) 327, 360
CviAII CATG 3 cut(s) 313, 424, 460
CviQI GTAC 2 cut(s) 327, 360
DdeI CTNAG 4 cut(s) 120, 291, 323, 410
DpnI GATC 1 cut(s) 43
DpnII GATC 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 335
EagI CGGCCG 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
EciI GGCGGA 1 cut(s) 193
EclXI CGGCCG 1 cut(s) 335
Eco47III AGCGCT 1 cut(s) 145
Eco52I CGGCCG 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 237
FaeI CATG 3 cut(s) 316, 427, 463
FaiI YATR 9 cut(s) 47, 285, 299, 314, 365, 425, 461, 467, 469
FatI CATG 3 cut(s) 312, 423, 459
Fnu4HI GCNGC 3 cut(s) 214, 338, 350
FokI GGATG 3 cut(s) 194, 250, 395
Fsp4HI GCNGC 3 cut(s) 214, 338, 350
FspBI CTAG 1 cut(s) 330
GlaI GCGC 1 cut(s) 145
GluI GCNGC 3 cut(s) 214, 338, 350
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 4 cut(s) 242, 277, 337, 349
HapII CCGG 2 cut(s) 278, 334
HhaI GCGC 1 cut(s) 146
Hin1II CATG 3 cut(s) 316, 427, 463
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 3 cut(s) 28, 50, 92
HpaII CCGG 2 cut(s) 278, 334
HphI GGTGA 1 cut(s) 230
Hpy188I TCNGA 1 cut(s) 33
Hpy99I CGWCG 1 cut(s) 37
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4V TGCA 1 cut(s) 312
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 260, 309, 346
HpyF3I CTNAG 4 cut(s) 120, 291, 323, 410
Hsp92II CATG 3 cut(s) 316, 427, 463
HspAI GCGC 1 cut(s) 144
KroI GCCGGC 1 cut(s) 333
KroNI GCCGGC 1 cut(s) 335
KspI CCGCGG 1 cut(s) 214
Kzo9I GATC 1 cut(s) 41
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 427
LweI GCATC 1 cut(s) 272
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 1 cut(s) 475
MalI GATC 1 cut(s) 43
MbiI CCGCTC 1 cut(s) 352
MboI GATC 1 cut(s) 41
MboII GAAGA 3 cut(s) 136, 140, 148
MflI RGATCY 1 cut(s) 41
MlyI GAGTC 3 cut(s) 37, 44, 86
MmeI TCCRAC 1 cut(s) 56
MnlI CCTC 3 cut(s) 6, 435, 463
MroNI GCCGGC 1 cut(s) 333
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 2 cut(s) 420, 481
MspA1I CMGCKG 1 cut(s) 213
MspI CCGG 2 cut(s) 278, 334
MspR9I CCNGG 1 cut(s) 239
Mva1269I GAATGC 1 cut(s) 393
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 4 cut(s) 73, 260, 309, 346
NaeI GCCGGC 1 cut(s) 335
NdeII GATC 1 cut(s) 41
NgoMIV GCCGGC 1 cut(s) 333
NlaIII CATG 3 cut(s) 316, 427, 463
NmeAIII GCCGAG 1 cut(s) 51
NspI RCATGY 1 cut(s) 316
PaeI GCATGC 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 153
PctI GAATGC 1 cut(s) 393
PdiI GCCGGC 1 cut(s) 335
PdmI GAANNNNTTC 1 cut(s) 228
PkrI GCNGC 3 cut(s) 215, 339, 351
PleI GAGTC 3 cut(s) 36, 44, 86
PpsI GAGTC 3 cut(s) 36, 44, 86
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspPI GGNCC 2 cut(s) 275, 347
PsuI RGATCY 1 cut(s) 41
RsaI GTAC 2 cut(s) 328, 361
RsaNI GTAC 2 cut(s) 327, 360
SacII CCGCGG 1 cut(s) 214
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 2 cut(s) 420, 481
SatI GCNGC 3 cut(s) 214, 338, 350
Sau3AI GATC 1 cut(s) 41
Sau96I GGNCC 2 cut(s) 275, 347
ScaI AGTACT 1 cut(s) 328
SchI GAGTC 3 cut(s) 37, 44, 86
ScrFI CCNGG 1 cut(s) 239
SetI ASST 7 cut(s) 69, 174, 305, 324, 411, 420, 455
SfaNI GCATC 1 cut(s) 272
Sfr303I CCGCGG 1 cut(s) 214
SgrBI CCGCGG 1 cut(s) 214
SphI GCATGC 1 cut(s) 316
SsiI CCGC 6 cut(s) 204, 211, 213, 216, 338, 350
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 197
TatI WGTACW 1 cut(s) 326
TauI GCSGC 3 cut(s) 216, 340, 352
Tru1I TTAA 2 cut(s) 420, 481
Tru9I TTAA 2 cut(s) 420, 481
TscAI CASTG 1 cut(s) 295
TspDTI ATGAA 1 cut(s) 237
TspRI CASTG 1 cut(s) 295
XceI RCATGY 1 cut(s) 316
XmnI GAANNNNTTC 1 cut(s) 228
XspI CTAG 1 cut(s) 330
ZrmI AGTACT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.